Krasnopolsky, V. A., Maillard, J. P. & Owen, T. C. Detection of methane in the martian atmosphere: evidence for life?. Icarus 172, 537–547 (2004).
Google Scholar
Formisano, V., Atreya, S., Encrenaz, T., Ignatiev, N. & Giuranna, M. Detection of methane in the atmosphere of mars. Science 306, 1758–1761 (2004).
Google Scholar
Geminale, A., Formisano, V. & Giuranna, M. Methane in Martian atmosphere: average spatial, diurnal, and seasonal behaviour. Planet. Space Sci. 56, 1194–1203 (2008).
Google Scholar
Mumma, M. J. et al. Strong release of methane on mars in northern summer 2003. Science 323, 1041–1045 (2009).
Google Scholar
Webster, C. R. et al. Mars methane detection and variability at Gale crater. Science 347, 415–417 (2015).
Google Scholar
Webster, C. R. et al. Background levels of methane in Mars’ atmosphere show strong seasonal variations. Science 360, 1093–1096 (2018).
Google Scholar
Korablev, O. et al. No detection of methane on Mars from early ExoMars Trace Gas Orbiter observations. Nature 568, 517–520 (2019).
Google Scholar
Fries, M. et al. A cometary origin for martian atmospheric methane. Geochem. Perspect. Lett. 2, 10–23 (2016).
Google Scholar
Keppler, F. et al. Ultraviolet-radiation-induced methane emissions from meteorites and the Martian atmosphere. Nature 486, 93–96 (2012).
Google Scholar
Moores, J. E. & Schuerger, A. C. UV degradation of accreted organics on Mars: IDP longevity, surface reservoir of organics, and relevance to the detection of methane in the atmosphere. J. Geophys. Res. Planets 117, E8 (2012).
Google Scholar
Schuerger, A. C., Moores, J. E., Clausen, C. A., Barlow, N. G. & Britt, D. T. Methane from UV-irradiated carbonaceous chondrites under simulated Martian conditions. J. Geophys. Res. Planets 117, E8 (2012).
Google Scholar
Etiope, G., Ehlmann, B. L. & Schoell, M. Low temperature production and exhalation of methane from serpentinized rocks on Earth: a potential analog for methane production on Mars. Icarus 224, 276–285 (2013).
Google Scholar
Oehler, D. Z. & Etiope, G. Methane seepage on mars: where to look and why. Astrobiology 17, 1233–1264 (2017).
Google Scholar
Onstott, T. C. et al. Martian CH 4: sources, flux, and detection. Astrobiology 6, 377–395 (2006).
Google Scholar
Elwood Madden, M. E., Ulrich, S. M., Onstott, T. C. & Phelps, T. J. Salinity-induced hydrate dissociation: A mechanism for recent CH4 release on Mars. Geophys. Res. Lett. https://doi.org/10.1029/2006GL029156 (2007).
Google Scholar
Conrad, R. The global methane cycle: recent advances in understanding the microbial processes involved. Environ. Microbiol. Rep. 1, 285–292 (2009).
Google Scholar
Kendrick, M. G. & Kral, T. A. Survival of methanogens during desiccation: implications for life on mars. Astrobiology 6, 546–551 (2006).
Google Scholar
Anderson, K. L., Apolinario, E. E. & Sowers, K. R. Desiccation as a long-term survival mechanism for the archaeon Methanosarcina barkeri. Appl. Environ. Microbiol. 78, 1473–1479 (2012).
Google Scholar
Kral, T. A. & Altheide, S. T. Methanogen survival following exposure to desiccation, low pressure and martian regolith analogs. Planet. Space Sci. 89, 167–171 (2013).
Google Scholar
Sowers, K. R. & Gunsalus, R. P. Adaptation for growth at various saline concentrations by the archaebacterium Methanosarcina thermophila. J. Bacteriol. 170, 998–1002 (1988).
Google Scholar
Maestrojuan, G. M. et al. Taxonomy and halotolerance of mesophilic methanosarcina strains, assignment of strains to species, and synonymy of methanosarcina mazei and methanosarcina frisia. Int. J. Syst. Bacteriol. 42, 561–567 (1992).
Google Scholar
Sowers, K. R., Boone, J. E. & Gunsalus, R. P. Disaggregation of methanosarcina spp and growth as single cells at elevated osmolarity. Appl. Environ. Microbiol. 59, 3832–3839 (1993).
Google Scholar
Sowers, K. R. & Gunsalus, R. P. Halotolerance in methanosarcina spp: Role of N(sup(epsilon))-Acetyl-(beta)-Lysine, (alpha)-Glutamate, Glycine Betaine, and K(sup+) as Compatible Solutes for Osmotic Adaptation. Appl. Environ. Microbiol. 61, 4382–4388 (1995).
Google Scholar
Roessler, M. et al. Identification of a salt-induced primary transporter for glycine betaine in the methanogen methanosarcina mazei go1. Appl. Environ. Microbiol. 68, 2133–2139 (2002).
Google Scholar
Shcherbakova, V., Oshurkova, V. & Yoshimura, Y. The effects of perchlorates on the permafrost methanogens: implication for autotrophic life on mars. Microorganisms 3, 518–534 (2015).
Google Scholar
Kral, T. A. et al. Sensitivity and adaptability of methanogens to perchlorates: Implications for life on Mars. Planet. Space Sci. 120, 87–95 (2016).
Google Scholar
Rivkina, E. M., Laurinavichus, K. S., Gilichinsky, D. A. & Shcherbakova, V. A. Methane generation in permafrost sediments. Dokl. Biol. Sci. https://doi.org/10.1023/A:1015366613580 (2002).
Google Scholar
Rivkina, E. et al. Microbial life in permafrost. Adv. Sp. Res. 33, 1215–1221 (2004).
Google Scholar
Rivkina, E. et al. Biogeochemistry of methane and methanogenic archaea in permafrost. FEMS Microbiol. Ecol. 61, 1–15 (2007).
Google Scholar
Takai, K. et al. Cell proliferation at 122 degrees C and isotopically heavy CH4 production by a hyperthermophilic methanogen under high-pressure cultivation. Proc. Natl. Acad. Sci. U. S. A. 105, 10949–10954 (2008).
Google Scholar
Sinha, N., Nepal, S., Kral, T. & Kumar, P. Survivability and growth kinetics of methanogenic archaea at various pHs and pressures: implications for deep subsurface life on Mars. Planet. Space Sci. 136, 15–24 (2017).
Google Scholar
Chastain, B. K. & Kral, T. A. Approaching mars-like geochemical conditions in the laboratory: omission of artificial buffers and reductants in a study of biogenic methane production on a Smectite clay. Astrobiology 10, 889–897 (2010).
Google Scholar
Kral, T. A., Altheide, T. S., Lueders, A. E. & Schuerger, A. C. Low pressure and desiccation effects on methanogens: Implications for life on Mars. Planet. Space Sci. 59, 264–270 (2011).
Google Scholar
Mickol, R. L. & Kral, T. A. Low pressure tolerance by methanogens in an aqueous environment: implications for subsurface life on mars. Orig. Life Evol. Biosph. 47, 511–532 (2017).
Google Scholar
Coates, J. D. & Achenbach, L. A. Microbial perchlorate reduction: rocket-fuelled metabolism. Nat. Rev. Microbiol. 2, 569–580 (2004).
Google Scholar
Ericksen, G. E. The Chilean Nitrate Deposits: The origin of the Chilean nitrate deposits, which contain a unique group of saline minerals, has provoked lively discussion for more than 100 years. Am. Sci. 71, 366–374 (1983).
Google Scholar
Kounaves, S. P. et al. Discovery of natural perchlorate in the antarctic dry valleys and its global implications. Environ. Sci. Technol. 44, 2360–2364 (2010).
Google Scholar
Hecht, M. H. et al. Detection of perchlorate and the soluble chemistry of Martian soil at the phoenix lander site. Science 325, 64–67 (2009).
Google Scholar
Navarro-González, R., Vargas, E., de la Rosa, J., Raga, A. C. & McKay, C. P. Reanalysis of the Viking results suggests perchlorate and organics at midlatitudes on Mars. J. Geophys. Res. 115, E12010 (2010).
Google Scholar
Glavin, D. P. et al. Evidence for perchlorates and the origin of chlorinated hydrocarbons detected by SAM at the Rocknest aeolian deposit in Gale Crater. J. Geophys. Res. Planets 118, 1955–1973 (2013).
Google Scholar
Kounaves, S. P. et al. Identification of the perchlorate parent salts at the Phoenix Mars landing site and possible implications. Icarus 232, 226–231 (2014).
Google Scholar
Kounaves, S. P., Carrier, B. L., O’Neil, G. D., Stroble, S. T. & Claire, M. W. Evidence of martian perchlorate, chlorate, and nitrate in Mars meteorite EETA79001: Implications for oxidants and organics. Icarus 229, 206–213 (2014).
Google Scholar
Ojha, L. et al. Spectral evidence for hydrated salts in recurring slope lineae on Mars. Nat. Geosci. https://doi.org/10.1038/ngeo2546 (2015).
Google Scholar
Clark, B. C. & Kounaves, S. P. Evidence for the distribution of perchlorates on Mars. Int. J. Astrobiol. 15, 311–318 (2016).
Google Scholar
Pestova, O. N., Myund, L. A., Khripun, M. K. & Prigaro, A. V. Polythermal study of the systems M(ClO4)2–H2O (M2+ = Mg2+, Ca2+, Sr2+, Ba2+). Russ. J. Appl. Chem. 78, 409–413 (2005).
Google Scholar
Chevrier, V. F., Hanley, J. & Altheide, T. S. Stability of perchlorate hydrates and their liquid solutions at the Phoenix landing site Mars. Geophys. Res. Lett. 36, L10202 (2009).
Google Scholar
Marion, G. M., Catling, D. C., Zahnle, K. J. & Claire, M. W. Modeling aqueous perchlorate chemistries with applications to Mars. Icarus 207, 675–685 (2010).
Google Scholar
Stillman, D. E. & Grimm, R. E. Dielectric signatures of adsorbed and salty liquid water at the Phoenix landing site Mars. J. Geophys. Res. 116, E09005 (2011).
Google Scholar
Toner, J. D., Catling, D. C. & Light, B. The formation of supercooled brines, viscous liquids, and low-temperature perchlorate glasses in aqueous solutions relevant to Mars. Icarus 233, 36–47 (2014).
Google Scholar
Nikolakakos, G. & Whiteway, J. A. Laboratory investigation of perchlorate deliquescence at the surface of Mars with a Raman scattering lidar. Geophys. Res. Lett. 42, 7899–7906 (2015).
Google Scholar
Maeder, D. L. et al. The Methanosarcina barkeri Genome: Comparative Analysis with Methanosarcina acetivorans and Methanosarcina mazei Reveals Extensive Rearrangement within Methanosarcinal Genomes. J. Bacteriol. 188, 7922–7931 (2006).
Google Scholar
Sorek, R. & Cossart, P. Prokaryotic transcriptomics: a new view on regulation, physiology and pathogenicity. Nat. Rev. Genet. 11, 9–16 (2010).
Google Scholar
Lobo, A. L. & Zinder, S. H. Diazotrophy and Nitrogenase Activity in the Archaebacterium Methanosarcina barkeri 227. Appl. Environ. Microbiol. 54, 1656–1661 (1988).
Google Scholar
Lobo, A. L. & Zinder, S. H. Nitrogenase in the archaebacterium Methanosarcina barkeri 227. J. Bacteriol. 172, 6789–6796 (1990).
Google Scholar
Kessler, P. S. & Leigh, J. A. Genetics of nitrogen regulation in Methanococcus maripaludis. Genetics 152, 1343–1351 (1999).
Google Scholar
Kessler, P. S., Daniel, C. & Leigh, J. A. Ammonia Switch-Off of Nitrogen Fixation in the Methanogenic Archaeon Methanococcus maripaludis: Mechanistic Features and Requirement for the Novel GlnB Homologues, NifI1 and NifI2. J. Bacteriol. 183, 882–889 (2001).
Google Scholar
Kempf, B. & Bremer, E. OpuA, an osmotically regulated binding protein-dependent transport system for the osmoprotectant glycine betaine in bacillus subtilis. J. Biol. Chem. 270, 16701–16713 (1995).
Google Scholar
Kempf, B. & Bremer, E. Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments. Arch. Microbiol. 170, 319–330 (1998).
Google Scholar
Hoffmann, T. & Bremer, E. Guardians in a stressful world: the Opu family of compatible solute transporters from Bacillus subtilis. Biol. Chem. 398, 193–214 (2017).
Google Scholar
Hippe, H., Caspari, D., Fiebig, K. & Gottschalk, G. Utilization of trimethylamine and other N-methyl compounds for growth and methane formation by Methanosarcina barkeri. Proc. Natl. Acad. Sci. 76, 494–498 (1979).
Google Scholar
Kreisl, P. & Kandler, O. Chemical structure of the cell wall polymer of methanosarcina. Syst. Appl. Microbiol. 7, 293–299 (1986).
Google Scholar
Jarrell, K. F., Jones, G. M., Kandiba, L., Nair, D. B. & Eichler, J. S-layer glycoproteins and flagellins: reporters of archaeal posttranslational modifications. Archaea 2010, 1–13 (2010).
Google Scholar
Srinivasan, G. Pyrrolysine encoded by UAG in archaea: charging of a UAG-decoding specialized tRNA. Science 296, 1459–1462 (2002).
Google Scholar
Bin, P., Huang, R. & Zhou, X. Oxidation resistance of the sulfur amino acids: methionine and cysteine. Biomed Res. Int. 2017, 1–6 (2017).
Google Scholar
Armesto, X. L., Canle, L. M., Fernández, M. I., Garcı́a, M. V. & Santaballa, J. A. First steps in the oxidation of sulfur-containing amino acids by hypohalogenation: very fast generation of intermediate sulfenyl halides and halosulfonium cations. Tetrahedron 56, 1103–1109 (2000).
Google Scholar
Casanueva, A., Tuffin, M., Cary, C. & Cowan, D. A. Molecular adaptations to psychrophily: the impact of ‘omic’ technologies. Trends Microbiol. 18, 374–381 (2010).
Google Scholar
Oren, A. Formation and breakdown of glycine betaine and trimethylamine in hypersaline environments. Antonie Van Leeuwenhoek 58, 291–298 (1990).
Google Scholar
Seibel, B. A. & Walsh, P. J. Trimethylamine oxide accumulation in marine animals: relationship to acylglycerol storage. J. Exp. Biol. 205, 297–306 (2002).
Google Scholar
Lobo, A. L. & Zinder, S. H. Nitrogen fixation by methanogenic bacteria. in Biological Nitrogen Fixation (eds. Stacey, G., Burris, R. H. & Evans, H. J.) 191–211 (Chapman and Hall, 1992).
Sohm, J. A., Webb, E. A. & Capone, D. G. Emerging patterns of marine nitrogen fixation. Nat. Rev. Microbiol. 9, 499–508 (2011).
Google Scholar
Bardiya, N. & Bae, J.-H. Dissimilatory perchlorate reduction: A review. Microbiol. Res. 166, 237–254 (2011).
Google Scholar
Barnum, T. P. et al. Genome-resolved metagenomics identifies genetic mobility, metabolic interactions, and unexpected diversity in perchlorate-reducing communities. ISME J. 12, 1568–1581 (2018).
Google Scholar
Oren, A., Elevi, B. R. & Mana, L. Perchlorate and halophilic prokaryotes: implications for possible halophilic life on Mars. Extremophiles 18, 75–80 (2014).
Google Scholar
Liebensteiner, M. G., Pinkse, M. W. H., Schaap, P. J., Stams, A. J. M. & Lomans, B. P. Archaeal (Per)Chlorate reduction at high temperature: an interplay of biotic and abiotic reactions. Science 340, 85–87 (2013).
Google Scholar
Bender, K. S. et al. Identification, characterization, and classification of genes encoding perchlorate reductase. J. Bacteriol. 187, 5090–5096 (2005).
Google Scholar
Youngblut, M. D. et al. Perchlorate reductase is distinguished by active site aromatic gate residues. J. Biol. Chem. 291, 9190–9202 (2016).
Google Scholar
Okeke, B. C., Giblin, T. & Frankenberger, W. T. Reduction of perchlorate and nitrate by salt tolerant bacteria. Environ. Pollut. https://doi.org/10.1016/S0269-7491(01)00288-3 (2002).
Google Scholar
He, L. et al. Biological perchlorate reduction: which electron donor we can choose?. Environ. Sci. Pollut. Res. 26, 16906–16922 (2019).
Google Scholar
Xie, T. et al. Perchlorate bioreduction linked to methane oxidation in a membrane biofilm reactor: performance and microbial community structure. J. Hazard. Mater. https://doi.org/10.1016/j.jhazmat.2018.06.011 (2018).
Google Scholar
Chaudhuri, S. K., O’Connor, S. M., Gustavson, R. L., Achenbach, L. A. & Coates, J. D. Environmental factors that control microbial perchlorate reduction. Appl. Environ. Microbiol. https://doi.org/10.1128/AEM.68.9.4425-4430.2002 (2002).
Google Scholar
Abu-Omar, M. M. Effective and catalytic reduction of perchlorate by atom transfer-reaction kinetics and mechanisms. Comments Inorg. Chem. 24, 15–37 (2003).
Google Scholar
Adkins, H. & Cramer, H. I. The use of nickel as a catalyst for hydrogenation. J. Am. Chem. Soc. 52, 4349–4358 (1930).
Google Scholar
Thauer, R. K. et al. Hydrogenases from methanogenic archaea, nickel, a novel cofactor, and H2 storage. Annu. Rev. Biochem. 79, 507–536 (2010).
Google Scholar
Zhang, H., Bruns, M. A. & Logan, B. E. Perchlorate reduction by a novel chemolithoautotrophic, hydrogen-oxidizing bacterium. Environ. Microbiol. https://doi.org/10.1046/j.1462-2920.2002.00338.x (2002).
Google Scholar
Ide, T., Bäumer, S. & Deppenmeier, U. Energy conservation by the H2: heterodisulfide oxidoreductase from methanosarcina mazei Gö1: identification of two proton-translocating segments. J. Bacteriol. 181, 4076–4080 (1999).
Google Scholar
Deppenmeier, U. The membrane-bound electron transport system of methanosarcina species. J. Bioenerg. Biomembr. 36, 55–64 (2004).
Google Scholar
Meuer, J., Kuettner, H. C., Zhang, J. K., Hedderich, R. & Metcalf, W. W. Genetic analysis of the archaeon Methanosarcina barkeri Fusaro reveals a central role for Ech hydrogenase and ferredoxin in methanogenesis and carbon fixation. Proc. Natl. Acad. Sci. 99, 5632–5637 (2002).
Google Scholar
Kulkarni, G., Mand, T. D. & Metcalf, W. W. Energy Conservation via Hydrogen Cycling in the Methanogenic Archaeon Methanosarcina barkeri. MBio 9, (2018).
Bobik, T. Formyl-methanofuran synthesis in Methanobacterium thermoautotrophicum. FEMS Microbiol. Lett. 87, 323–326 (1990).
Google Scholar
Wang, D. M., Shah, S. I., Chen, J. G. & Huang, C. P. Catalytic reduction of perchlorate by H2 gas in dilute aqueous solutions. Sep. Purif. Technol. 60, 14–21 (2008).
Google Scholar
Thauer, R. K., Kaster, A.-K., Seedorf, H., Buckel, W. & Hedderich, R. Methanogenic archaea: ecologically relevant differences in energy conservation. Nat. Rev. Microbiol. 6, 579–591 (2008).
Google Scholar
Mand, T. D. & Metcalf, W. W. Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the Genus Methanosarcina. Microbiol. Mol. Biol. Rev. 83, (2019).
Rummel, J. D. et al. A new analysis of mars “special regions”: findings of the second MEPAG special regions science analysis group (SR-SAG2). Astrobiology 14, 887–968 (2014).
Google Scholar
Bryant, M. P. & Boone, D. R. Emended description of strain MST(DSM 800T), the type strain of methanosarcina barkeri. Int. J. Syst. Bacteriol. 37, 169–170 (1987).
Google Scholar
Widdel, F., Kohring, G.-W. & Mayer, F. Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. Arch. Microbiol. 134, 286–294 (1983).
Google Scholar
Francisco, D. E., Mah, R. A. & Rabin, A. C. Acridine orange-epifluorescence technique for counting bacteria in natural waters. Trans. Am. Microsc. Soc. 92, 416 (1973).
Google Scholar
Chen, S., Zhou, Y., Chen, Y. & Gu, J. fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics 34, i884–i890 (2018).
Google Scholar
Langmead, B. & Salzberg, S. L. Fast gapped-read alignment with Bowtie 2. Nat. Methods 9, 357–359 (2012).
Google Scholar
Li, H. et al. The sequence alignment/map format and SAMtools. Bioinformatics 25, 2078–2079 (2009).
Google Scholar
Quinlan, A. R. & Hall, I. M. BEDTools: a flexible suite of utilities for comparing genomic features. Bioinformatics 26, 841–842 (2010).
Google Scholar
Camacho, C. et al. BLAST+: architecture and applications. BMC Bioinformatics 10, 421 (2009).
Google Scholar
Love, M., Anders, S. & Huber, W. Differential analysis of count data–the DESeq2 package. Genome Biol. 15, 10–1186 (2014).
Google Scholar
Ogata, H. et al. KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 27, 29–34 (1999).
Google Scholar
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