Abstract
This work evaluated the effect of culture temperature and irradiance on oxygen production rate and biomass productivity of the microalga Tetradesmus bajacalifornicus, an understudied species with potential for different biotechnological applications. The optimal conditions to increase oxygen productivity were determined using a photorespirometer using a response surface methodology. The highest oxygen production rate (250.0 mg O₂·g⁻¹·h⁻¹) was achieved at 38.1 °C and 500 µmol photons·m⁻²·s⁻¹, a value comparable to other high-performance strains. However, continuous exposure to 38.1 °C for extended periods led to photodamage and culture collapse, indicating the importance of balancing peak oxygen production rates with thermal tolerance. Further experiments showed that heating the culture for 1 h per day at 38.1 °C enhanced biomass accumulation by 12.5%, but longer exposures reduced oxygen production efficiency and growth. Milder temperatures (29.0 °C) did not alter oxygen production even with prolonged exposures, while 34.0 °C became detrimental beyond 1 h. These findings highlight the need to incorporate both temperature magnitude and exposure duration into microalgal growth models. Furthermore, Tetradesmus bajacalifornicus demonstrated robust adaptability to high irradiance and moderate thermal stress, making it a promising candidate for outdoor cultivation in warm, high-radiation environments.
Data availability
The authors declare that the data supporting the findings of this study are available within the paper. Should any raw data files be needed in another format they are available from the corresponding author upon reasonable request.
References
Feng, Y. et al. Using high CO2 concentrations to culture microalgae for lipid and fatty acid production: Synthesis based on a meta-analysis. Aquaculture 594, 741386 (2025).
Viviano, E. & Limongi, A. R. Elsevier,. Production of biofuels from microalgae. in Sustainable Industrial Processes Based on Microalgae 297–321 (2023). https://doi.org/10.1016/B978-0-443-19213-5.00013-3
Sadvakasova, A. K. et al. Strategies to intensify CO2 capture by microalgae for the circular bioeconomy. Trends Plant. Sci. https://doi.org/10.1016/J.TPLANTS.2025.10.012 (2025).
Zhang, X. et al. Microalgal capture of carbon dioxide: A carbon sink or source? Bioresour. Technol. 390, 129824 (2023).
De Luca, M. et al. Lipids from Microalgae for Cosmetic Applications. Cosmetics 2021. 8, 52 (2021).
Morillas-España, A., Lafarga, T., Sánchez-Zurano, A., Acién-Fernández, F. G. & González-López, C. Microalgae based wastewater treatment coupled to the production of high value agricultural products: Current needs and challenges. Chemosphere 291, 132968 (2022).
Morillas-España, A., Lafarga, T., Gómez-Serrano, C., Acién-Fernández, F. G. & González-López, C. V. Year-long production of Scenedesmus almeriensis in pilot-scale raceway and thin-layer cascade photobioreactors. Algal Res. 51, 102069 (2020).
Richardson, J. W., Johnson, M. D. & Outlaw, J. L. Economic comparison of open pond raceways to photo bio-reactors for profitable production of algae for transportation fuels in the Southwest. Algal Res. 1, 93–100 (2012).
Banerjee, S. & Ramaswamy, S. Comparison of productivity and economic analysis of microalgae cultivation in open raceways and flat panel photobioreactor. Bioresour Technol. Rep 8, 100328 (2019).
Villaró, S. et al. Microalgae derived astaxanthin: Research and consumer trends and industrial use as food. Foods vol. 10 Preprint at (2021). https://doi.org/10.3390/foods10102303
Schulze, P. S. C., Guerra, R., Pereira, H., Schüler, L. M. & Varela, J. C. S. Flashing LEDs for Microalgal Production. Trends in Biotechnology 35, 1088–1101 (2017). https://doi.org/10.1016/j.tibtech.2017.07.011
Maltsev, Y., Maltseva, K., Kulikovskiy, M. & Maltseva, S. Influence of Light Conditions on Microalgae Growth and Content of Lipids, Carotenoids, and Fatty Acid Composition. Biology (Basel). 10, 1060 (2021).
Cheng, H. H., Huang, D. S. & Lin, M. T. Heat dissipation design and analysis of high power LED array using the finite element method. Microelectron. Reliab. 52, 905–911 (2012).
Glemser, M. et al. Application of light-emitting diodes (LEDs) in cultivation of phototrophic microalgae: current state and perspectives. Appl. Microbiol. Biotechnol. 100, 1077–1088 (2016).
Cerdá-Moreno, C. et al. Effect of temperature on the oxygen production capacity and growth of scenedesmus almeriensis. Algal Res 84, 103795 (2024).
Sánchez-Zurano, A., Gómez-Serrano, C., Acién-Fernández, F. G. & Fernández-Sevilla, J. M. & Molina-Grima, E. A novel photo-respirometry method to characterize consortia in microalgae-related wastewater treatment processes. Algal Res 47, 101858 (2020).
Patil, L. & Kaliwal, B. B. Microalga Scenedesmus bajacalifornicus BBKLP-07, a new source of bioactive compounds with in vitro pharmacological applications. Bioprocess. Biosyst. Eng. 42, 979–994 (2019).
Patil, L. & Kaliwal, B. Effect of CO2 concentration on growth and biochemical composition of newly isolated indigenous microalga Scenedesmus bajacalifornicus BBKLP-07. Appl. Biochem. Biotechnol. 182, 335–348 (2017).
Rocha, D. N. et al. Combination of trace elements and salt stress in different cultivation modes improves the lipid productivity of Scenedesmus spp. Bioresour. Technol. 289, 121644 (2019).
Valero-Vizcaino, A., Villaró-Cos, S., Morillas-España, A., Cerdá-Moreno, C. & Lafarga, T. Production of techno-functional proteins and plant biostimulants from Nannochloropsis gaditana. Food Biosci. 59, 104000 (2024).
Krzemińska, I., Pawlik-Skowrońska, B., Trzcińska, M. & Tys, J. Influence of photoperiods on the growth rate and biomass productivity of green microalgae. Bioprocess. Biosyst. Eng. 37, 735–741 (2014).
Yin, S. et al. Factors affect the oxygen production of Chlorella pyrenoidosa in a bacterial-algal symbiotic system: Light intensity, temperature, pH and static magnetic field. Process Saf. Environ. Prot. 184, 492–501 (2024).
Valero-Vizcaino, A., Villaró-Cos, S., Morillas-España, A., Cerdá-Moreno, C. & Lafarga, T. Production of techno-functional proteins and plant biostimulants from Nannochloropsis gaditana. Food Biosci. 59, 104000 (2024).
Cho, H. S. & Lee, J. M. Taxonomic reinvestigation of the genus Tetradesmus (Scenedesmaceae; Sphaeropleales) based on morphological characteristics and chloroplast genomes. Front. Plant Sci. 15, 1303175 (2024).
Barten, R. et al. Short-term physiologic response of the green microalga Picochlorum sp. (BPE23) to supra-optimal temperature. Sci. Rep. 12, 3290 (2022).
Wang, Y., He, B., Sun, Z. & Chen, Y. F. Chemically enhanced lipid production from microalgae under low sub-optimal temperature. Algal Res. 16, 20–27 (2016).
Sánchez, J. F. et al. Biomass and lutein productivity of Scenedesmus almeriensis: Influence of irradiance, dilution rate and temperature. Appl. Microbiol. Biotechnol. 79, 719–729 (2008).
Barati, B., Gan, S. Y., Lim, P. E., Beardall, ·, J. & Phang, S. M. Green algal molecular responses to temperature stress. Acta Physiol. Plant. 41, 26 (2019).
Courcoul, C. et al. Disentangling the effects of microalgal diversity and thermal history on freshwater phototrophic biofilms facing heat stress: A thermal dose approach. J. Ecol. 111, 773–786 (2023).
Los, D. A., Kirill, •, Mironov, S. & Allakhverdiev, S. I. Regulatory role of membrane fluidity in gene expression and physiological functions. Photosynth. Res. 116, 489-509
Schroda, M., Hemme, D. & Mühlhaus, T. The Chlamydomonas heat stress response. Plant J. 82, 466–480 (2015).
Yokthongwattana, K., Chrost, B., Behrman, S., Casper-Lindley, C. & Melis, A. Photosystem II damage and repair cycle in the green alga Dunaliella salina: Involvement of a Chloroplast-Localized HSP70. Plant Cell Physiol. 42, 1389–1397 (2001).
Zuppini, A., Andreoli, C. & Baldan, B. Heat stress: An inducer of programmed cell death in Chlorella saccharophila. Plant. Cell. Physiol. 48, 1000–1009 (2007).
Funding
This work forms part of the SOLAR·FOODS (PID2022-136292OB-I00) and SHAPE (CNS2024-154218) projects, funded by the Spanish Ministry of Science and Innovation. The authors also acknowledge the financial support given by the RE·USE (PLSQ_2023_00233) and BLUE·FUTURE (PCM_00083) projects, funded by the Government of Andalusia and the European Regional Development Fund. The authors would also like to thank PPIT-UAL, Junta de Andalucia-FEDER 2021–2027 (CPRE2023-076) and the Ramon y Cajal Program (RYC2021-031061-I).
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S. Villaró : Investigation, Formal analysis, and Writing – Original draft; C. Cerdá-Moreno : Investigation, Formal analysis, and Writing – Original draft; E. Viviano: Formal analysis, Writing – Original draft; J. Tripiana : Investigation; S. Triviño de las Heras : Investigation; M. Salinas-García : Investigation; T. Lafarga : Writing – Review & editing, Supervision and Funding acquisition.
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Villaró-Cos, S., Cerdá-Moreno, C., Viviano, E. et al. Optimising thermal and irradiance conditions for enhanced oxygen production in Tetradesmus bajacalifornicus.
Sci Rep (2026). https://doi.org/10.1038/s41598-026-41958-6
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DOI: https://doi.org/10.1038/s41598-026-41958-6
Keywords
- Photorespirometry
- Biomass
- Algae
- Photosynthesis
- Renewable resources
- Carbon dioxide
Source: Ecology - nature.com
