Abstract
Culex quinquefasciatus is a major vector of West Nile virus and other pathogens, yet genetic population suppression tools for this species remain limited. Here, we develop a self-limiting, CRISPR-based suppression gene drive system targeting doublesex, close to the male-determining locus, promoting male transmission. A recoded dsxM sequence converts females into sterile intersexes, preventing population-level spread. The drive achieves super-Mendelian inheritance ( ~ 71%) and generates resistance alleles that are fully or partially dominant female sterile. Single-release cage trials show extended but self-limiting population suppression. Population modeling of this RIDD (Release of Insects carrying a Dominant-sterile Drive) system further indicates that repeated releases can substantially reduce fertile female numbers at low release ratios and intrinsic growth rates, outperforming non-drive strategies under comparable conditions. Together, these results establish a self-limiting suppression gene drive platform for Culex, providing a confined and sustainable framework for vector population control.
Acknowledgments
The research reported in this manuscript was supported by Zhejiang University, Key Laboratory of Biology of Crop Pathogen and Insects of Zhejiang Province, College of Agricultural and Biotechnology, China; by Shenzhen Bay laboratory, Shenzhen, China; and by the Center for Life Sciences, Peking University, Beijing, China.
Funding
X.F. discloses support for the research of this work from the National Natural Science Foundation of China [grant number 82202559], Zhejiang Province Science Foundation [grant number MS25C140016]. F.L. discloses support for publication of this work from the National Natural Science Foundation of China [grant number 82372289]. J.C. discloses support for the research of this work from the National Natural Science Foundation of China [grant number 32270672].
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Feng, X., Ding, J., Liu, Y. et al. Self-limiting population suppression gene drive design in the West Nile vector mosquito, Culex quinquefasciatus.
Nat Commun (2026). https://doi.org/10.1038/s41467-026-73641-9
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DOI: https://doi.org/10.1038/s41467-026-73641-9
Source: Ecology - nature.com
