The coexistence of r and K strategy in a unicellular microalga Haematococcus lacustris

1. He, Z., Webster, S. & He, S. Y. Growth–defense trade-offs in plants. Curr. Biol. 32, R634–R639 (2022).

   Google Scholar 

2. Smith, W. P. J., Wucher, B. R., Nadell, C. D. & Foster, K. R. Bacterial defences: mechanisms, evolution and antimicrobial resistance. Nat. Rev. Microbiol 21, 519–534 (2023).

   Google Scholar 

3. Lean, J. L. & Rind, D. H. How natural and anthropogenic influences alter global and regional surface temperatures: 1889 to 2006. Geophys. Res. Lett. 35, L18701 (2008).

4. Omer, A., Zhuguo, M., Zheng, Z. & Saleem, F. Natural and anthropogenic influences on the recent droughts in Yellow River Basin, China. Sci. Total Environ. 704, 135428 (2020).

   Google Scholar 

5. Andrews, J. H. & Harris, R. F. r- and K-Selection and Microbial Ecology. in Advances in Microbial Ecology (ed. Marshall, K. C.) vol. 9 99–147 (Springer US, 1986).

6. Hengeveld, R. The theory of island biogeography. Acta Biotheor. 50, 133–136 (2002).

   Google Scholar 

7. Stearns, S. C. Life-history tactics: a review of the ideas. Q. Rev. Biol. 51, 3–47 (1976).

   Google Scholar 

8. Malik, A. A. et al. Defining trait-based microbial strategies with consequences for soil carbon cycling under climate change. ISME J. 14, 1–9 (2020).

   Google Scholar 

9. Richardson, B. J. r and K selection in kangaroos. Nature 255, 323–324 (1975).

   Google Scholar 

10. Schmidt, W. & Briibach, M. Plant distribution patterns during early succession on an artificial protosoil. J. Veg. Sci. 4, 247–254 (1993).

    Google Scholar 

11. Yin, Q., Sun, Y., Li, B., Feng, Z. & Wu, G. The r/K selection theory and its application in biological wastewater treatment processes. Sci. Total Environ. 824, 153836 (2022).

    Google Scholar 

12. Sinervo, B., Svensson, E. & Comendant, T. Density cycles and an offspring quantity and quality game driven by natural selection. Nature 406, 985–988 (2000).

    Google Scholar 

13. Cheng, Y. et al. Next generation modeling of microbial souring – parameterization through genomic information. Int. Biodeterior. Biodegrad. 126, 189–203 (2018).

    Google Scholar 

14. Pianka, E. R. On r- and K-selection. Am. Nat. 104, 592–597 (1970).

    Google Scholar 

15. Fierer, N., Bradford, M. A. & Jackson, R. B. Toward an ecological classification of soil bacteria. Ecology 88, 1354–1364 (2007).

    Google Scholar 

16. Souissi, A., Hwang, J.-S. & Souissi, S. Reproductive trade-offs of the estuarine copepod Eurytemora affinis under different thermal and haline regimes. Sci. Rep. 11, 20139 (2021).

    Google Scholar 

17. Fierer, N. et al. Comparative metagenomic, phylogenetic and physiological analyses of soil microbial communities across nitrogen gradients. ISME J. 6, 1007–1017 (2012).

    Google Scholar 

18. Jones, S. E. & Lennon, J. T. Dormancy contributes to the maintenance of microbial diversity. Proc. Natl. Acad. Sci. USA 107, 5881–5886 (2010).

    Google Scholar 

19. Figueroa, R. I., Estrada, M. & Garcés, E. Life histories of microalgal species causing harmful blooms: haploids, diploids and the relevance of benthic stages. Harmful Algae 73, 44–57 (2018).

    Google Scholar 

20. Lennon, J. T. & Jones, S. E. Microbial seed banks: the ecological and evolutionary implications of dormancy. Nat. Rev. Microbiol 9, 119–130 (2011).

    Google Scholar 

21. Chekanov, K., Fedorenko, T., Kublanovskaya, A., Litvinov, D. & Lobakova, E. Diversity of carotenogenic microalgae in the White Sea polar region. FEMS Microbiol. Ecol. 96, fiz183 (2019).

22. Kublanovskaya, A., Baulina, O., Chekanov, K. & Lobakova, E. The microalga Haematococcus lacustris (Chlorophyceae) forms natural biofilms in supralittoral White Sea coastal rock ponds. Planta 252, 37 (2020).

    Google Scholar 

23. Tang, S. et al. Bet hedging in a unicellular microalga. Nat. Commun. 15, 2063 (2024).

    Google Scholar 

24. Royer, M., Larbat, R., Le Bot, J., Adamowicz, S. & Robin, C. Is the C:N ratio a reliable indicator of C allocation to primary and defence-related metabolisms in tomato? Phytochemistry 88, 25–33 (2013).

    Google Scholar 

25. Houston, K., Tucker, M. R., Chowdhury, J., Shirley, N. & Little, A. The plant cell wall: a complex and dynamic structure as revealed by the responses of genes under stress conditions. Front. Plant Sci. 7, 984 (2016).

26. Kominami, S., Mizuta, H. & Uji, T. Transcriptome profiling in the marine red alga neopyropia yezoensis under light/dark cycle. Mar. Biotechnol. 24, 393–407 (2022).

    Google Scholar 

27. Dai, X. & Zhu, M. Coupling of ribosome synthesis and translational capacity with cell growth. Trends Biochem. Sci. 45, 681–692 (2020).

    Google Scholar 

28. Chen, J. et al. Change in composition and potential functional genes of microbial communities on carbonatite rinds with different weathering times. Front. Microbiol. 13, 1024672 (2022).

29. Peng, J. et al. Shift in soil bacterial communities from K- to r-strategists facilitates adaptation to grassland degradation. Land Degrad. Dev. 33, 2076–2091 (2022).

    Google Scholar 

30. Shimakawa, G. et al. Diverse strategies of O₂ usage for preventing photo-oxidative damage under CO₂ limitation during algal photosynthesis. Sci. Rep. 7, 41022 (2017).

    Google Scholar 

31. Stein, L. Y. & Klotz, M. G. The nitrogen cycle. Curr. Biol. 26, R94–R98 (2016).

    Google Scholar 

32. Robyt, J. F. Starch: structure, properties, chemistry, and enzymology. in Glycoscience: Chemistry and Chemical Biology (eds Fraser-Reid, B. O., Tatsuta, K. & Thiem, J.) 1437–1472 https://doi.org/10.1007/978-3-540-30429-6_35 (Springer, 2008).

33. Utting, S. D. Influence of nitrogen availability on the biochemical composition of three unicellular marine algae of commercial importance. Aquacult. Eng. 4, 175–190 (1985).

    Google Scholar 

34. Richardson, B., Orcutt, D. M., Schwertner, H. A., Martinez, C. L. & Wickline, H. E. Effects of nitrogen limitation on the growth and composition of unicellular algae in continuous culture. Appl. Microbiol. 18, 245–250 (1969).

    Google Scholar 

35. Giraldo, N. D. et al. Reducing self-shading effects in Botryococcus braunii cultures: effect of Mg2+ deficiency on optical and biochemical properties, photosynthesis and lipidomic profile. Bioresour. Bioprocess. 8, 33 (2021).

    Google Scholar 

36. Hadj-Romdhane, F. et al. The culture of Chlorella vulgaris in a recycled supernatant: effects on biomass production and medium quality. Bioresour. Technol. 132, 285–292 (2013).

    Google Scholar 

37. Kobayashi, M., Kurimura, Y., Kakizono, T., Nishio, N. & Tsuji, Y. Morphological changes in the life cycle of the green alga Haematococcus pluvialis. J. Ferment. Bioeng. 84, 94–97 (1997).

    Google Scholar 

38. Wang, N., Guan, B., Kong, Q. & Duan, L. A semi-continuous cultivation method for Haematococcus pluvialis from non-motile cells to motile cells. J. Appl. Phycol. 30, 773–781 (2018).

    Google Scholar 

39. Tang, Y. Z. et al. Exploration of resting cysts (stages) and their relevance for possibly HABs-causing species in China. Harmful Algae 107, 102050 (2021).

    Google Scholar 

40. Rengefors, K., Karlsson, I. & Hansson, L. Algal cyst dormancy: a temporal escape from herbivory. Proc. R. Soc. Lond. B 265, 1353–1358 (1998).

    Google Scholar 

41. Ellegaard, M. & Ribeiro, S. The long-term persistence of phytoplankton resting stages in aquatic ‘seed banks’. Biol. Rev. 93, 166–183 (2018).

    Google Scholar 

42. Dahms, H.-U. Dormancy in the Copepoda? An overview. Hydrobiologia 306, 199–211 (1995).

    Google Scholar 

43. Yang, J. et al. Cell size and growth rate are major determinants of replicative lifespan. Cell Cycle 10, 144–155 (2011).

    Google Scholar 

44. Boussiba, S. Carotenogenesis in the green alga Haematococcus pluvialis: cellular physiology and stress response. Physiol. Plant 108, 111–117 (2000).

    Google Scholar 

45. Wayama, M. et al. Three-dimensional ultrastructural study of oil and astaxanthin accumulation during encystment in the green alga Haematococcus pluvialis. PLoS ONE 8, e53618 (2013).

    Google Scholar 

46. Lakshmi Narasimhan, A. et al. Enhanced astaxanthin production in Haematococcus lacustris by electrochemical stimulation of cyst germination. Bioresour. Technol. 411, 131301 (2024).

    Google Scholar 

47. Genovesi, B. et al. Dormancy and germination features in resting cysts of Alexandrium tamarense species complex (Dinophyceae) can facilitate bloom formation in a shallow lagoon (Thau, southern France). J. Plankton Res. 31, 1209–1224 (2009).

48. Marinho, Y. F. et al. Evaluation of the influence of different culture media on growth, life cycle, biochemical composition, and astaxanthin production in Haematococcus pluvialis. Aquacult Int. 29, 757–778 (2021).

    Google Scholar 

49. Bi, H. et al. Temporal characteristics of plankton indicators in coastal waters: high-frequency data from PlanktonScope. J. Sea Res. 189, 102283 (2022).

    Google Scholar 

50. He, K., Gkioxari, G., Dollar, P. & Girshick, R. Mask R-CNN. IEEE Trans. Pattern Anal. Mach. Intell. 42, 386–397 (2020).

    Google Scholar 

51. Yan, Q. et al. Internal nutrient loading is a potential source of eutrophication in Shenzhen Bay, China. Ecol. Indic. 127, 107736 (2021).

    Google Scholar 

52. Yan, Y. et al. Soil organic carbon and total nitrogen in intensively managed arable soils. Agric. Ecosyst. Environ. 150, 102–110 (2012).

    Google Scholar 

53. Lu, Z., Zheng, L., Liu, J., Dai, J. & Song, L. A novel fed-batch strategy to boost cyst cells production based on the understanding of intracellular carbon and nitrogen metabolism in Haematococcus pluvialis. Bioresour. Technol. 289, 121744 (2019).

    Google Scholar 

54. Brányiková, I. et al. Microalgae—novel highly efficient starch producers. Biotechnol. Bioeng. 108, 766–776 (2011).

    Google Scholar 

55. Zhi, M. et al. Filamentous cyanobacteria and hydrophobic protein in extracellular polymeric substances facilitate algae—bacteria aggregation during partial nitrification. Int. J. Biol. Macromol. 251, 126379 (2023).

    Google Scholar 

56. Wang, Q.-R., Hong, Y. & Li, L.-H. Insights into differences between spore-assisted and pellet-assisted microalgae harvesting using a highly efficient fungus: efficiency, high-value substances, and mechanisms. Sci. Total Environ. 877, 162945 (2023).

    Google Scholar 

57. Pertea, M. et al. StringTie enables improved reconstruction of a transcriptome from RNA-seq reads. Nat. Biotechnol. 33, 290–295 (2015).

    Google Scholar 

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