The ecological and developmental foundations of brood parasitism in a catfish

1. Davies, N. Cuckoos, Cowbirds and Other Cheats (AC Black, 2010).

2. Feeney, W. E. et al. Brood parasitism and the evolution of cooperative breeding in birds. Science 342, 1506–1508 (2013).

   Google Scholar 

3. Feeney, W. E., Welbergen, J. A. & Langmore, N. E. Advances in the study of coevolution between avian brood parasites and their hosts. Annu. Rev. Ecol. Evol. Syst. 45, 227–246 (2014).

   Google Scholar 

4. Kilner, R. M. & Langmore, N. E. Cuckoos versus hosts in insects and birds: adaptations, counter-adaptations and outcomes. Biol. Rev. 86, 836–852 (2011).

   Google Scholar 

5. Medina, I. & Langmore, N. E. The evolution of acceptance and tolerance in hosts of avian brood parasites. Evolution of defences in hosts. Biol. Rev. 91, 569–577 (2016).

   Google Scholar 

6. Thorogood, R., Spottiswoode, C. N., Portugal, S. J. & Gloag, R. The coevolutionary biology of brood parasitism: a call for integration. Philos. Trans. R. Soc. B Biol. Sci. 374, 20180190 (2019).

   Google Scholar 

7. Lyon, B. E. & Eadie, J. McA. Conspecific brood parasitism in birds: a life-story perspective. Annu. Rev. Ecol. Evol. Syst. 39, 343–363 (2008).

   Google Scholar 

8. Spottiswoode, C. N., Kilner, R. M. & Davies, N. B. Brood parasitism. In The Evolution of Parental Care 226–356. (Oxford University Press, Oxford, 2012).

9. Sless, T. J. L., Danforth, B. N. & Searle, J. B. Evolutionary origins and patterns of diversification in animal brood parasitism. Am. Nat. 202, 107–121 (2023).

   Google Scholar 

10. Skelton, P. Freshwater Fishes of Southern Africa (Struik Nature, 2024).

11. Sato, T. A brood parasitic catfish of mouthbrooding cichlid fishes in Lake Tanganyika. Nature 323, 58–59 (1982).

    Google Scholar 

12. Ronco, F. et al. Drivers and dynamics of a massive adaptive radiation in cichlid fishes. Nature 589, 76–81 (2021).

    Google Scholar 

13. Blažek, R. et al. Success of cuckoo catfish brood parasitism reflects coevolutionary history and individual experience of their cichlid hosts. Sci. Adv. 4, eaar4380 (2018).

    Google Scholar 

14. Wisenden, B. D. Alloparental care in fishes. Rev. Fish Biol. Fish. 9, 45–70 (1999).

    Google Scholar 

15. Reichard, M. Cuckoo catfish. Curr. Biol. 29, R722–R723 (2019).

    Google Scholar 

16. Zimmermann, H., Blažek, R., Polačik, M. & Reichard, M. Individual experience as a key to success for the cuckoo catfish brood parasitism. Nat. Commun. 13, 1723 (2022).

    Google Scholar 

17. Cohen, M. S., Hawkins, M. B., Stock, D. W. & Cruz, A. Early life-history features associated with brood parasitism in the cuckoo catfish, Synodontis multipunctatus (Siluriformes: Mochokidae). Philos. Trans. R. Soc. B Biol. Sci. 374, 20180205 (2019).

    Google Scholar 

18. Zimmerman, H., Tolman, D. & Reichard, M. Low incidence of cannibalism among brood parasitic cuckoo catfish embryos. Behav. Ecol. 34, 521–527 (2023).

    Google Scholar 

19. Mouginot, P., Galipaud, M. & Reichard, M. The evolution of brood parasitism from host egg predation. Behav. Ecol. 35, arae043 (2024).

    Google Scholar 

20. Day, J. J. & Wilkinson, M. On the origin of the Synodontis catfish species flock from Lake Tanganyika. Biol. Lett. 2, 548–552 (2006).

    Google Scholar 

21. Koblmüller, S., Egger, B., Sturmbauer, C. & Sefc, K. M. Rapid radiation, ancient incomplete lineage sorting and ancient hybridization in the endemic Lake Tanganyika cichlid tribe Tropheini. Mol. Phylogenet. Evol. 55, 318–334 (2010).

    Google Scholar 

22. Englmaier, G. K. et al. Revised taxonomy of Synodontis catfishes (Siluriformes: Mochokidae) from the Lake Tanganyika basin reveals lower species diversity than expected. Zool. J. Linn. Soc. 202, zlae130 (2024).

    Google Scholar 

23. Wright, J. J. & Page, L. M. Taxonomic revision of Lake Tanganyikan Synodontis (Siluriformes: Mochokidae). Bull. Fla. Mus. Nat. Hist. 46, 57 (2006).

    Google Scholar 

24. Peart, C. R., Bills, R., Newton, J., Near, T. J. & Day, J. J. Do sympatric catfish radiations in Lake Tanganyika show eco-morphological diversification? Evol. J. Linn. Soc. 3, kzae015 (2024).

    Google Scholar 

25. Schluter, D. The Ecology of Adaptive Radiation (Oxford University Press, Oxford, 2000).

26. Stroud, J. T. & Losos, J. B. Ecological opportunity and adaptive radiation. Annu. Rev. Ecol. Evol. Syst. 47, 507–532 (2016).

    Google Scholar 

27. Reichard, M. et al. Lack of host specialization despite selective host use in brood parasitic cuckoo catfish. Mol. Ecol. 32, 6070–6082 (2023).

    Google Scholar 

28. Viertler, A., Salzburger, W. & Ronco, F. Comparative scale morphology in the adaptive radiation of cichlid fishes (Perciformes: Cichlidae) from Lake Tanganyika. Biol. J. Linn. Soc. 134, 541–556 (2021).

29. Jawad, L. A. Comparative morphology of scales of four teleost fishes from Sudan and Yemen. J. Nat. Hist. 39, 2643–2660 (2005).

    Google Scholar 

30. Muschick, M., Indermaur, A. & Salzburger, W. Convergent evolution within an adaptive radiation of cichlid fishes. Curr. Biol. 22, 2362–2368 (2012).

    Google Scholar 

31. Nair, P., Miller, C. M. & Fuiman, L. A. Tracing exploitation of egg boons: an experimental study using fatty acids and stable isotopes. J. Exp. Biol. 226, jeb246247 (2023).

    Google Scholar 

32. Hunter, J. R. & Kimbrell, C. A. Egg cannibalism in the Northern anchovy, Engraulis mordax. Fish. Bull. 78, 811–816 (1980).

    Google Scholar 

33. Yongo, E., Iteba, J. & Agember, S. Review of food and feeding habits of some Synodontis fishes in African freshwaters. Oceanogr. Fish. Open Access J. 10, 27–31 (2019).

34. Konings, A. Tanganyika Cichlids in Their Natural Habitat (Cichlid Press, El Paso, 2015).

35. Stoddard, M. C. & Hauber, M. E. Colour, vision and coevolution in avian brood parasitism. Philos. Trans. R. Soc. B Biol. Sci. 372, 20160339 (2017).

    Google Scholar 

36. Santos, M. E. et al. The evolution of cichlid fish egg-spots is linked with a cis-regulatory change. Nat. Commun. 5, 5149 (2014).

    Google Scholar 

37. Atukorala, A. D. S. & Franz-Odendaal, T. A. Spatial and temporal events in tooth development of Astyanax mexicanus. Mech. Dev. 134, 42–54 (2014).

    Google Scholar 

38. Debiais-Thibaud, M. et al. Development of oral and pharyngeal teeth in the medaka (Oryzias latipes): comparison of morphology and expression of eve1 gene. J. Exp. Zoolog. B Mol. Dev. Evol. 308B, 693–708 (2007).

    Google Scholar 

39. Koblmüller, S., Sturmbauer, C., Verheyen, E., Meyer, A. & Salzburger, W. Mitochondrial phylogeny and phylogeography of East African squeaker catfishes (Siluriformes: Synodontis). BMC Evol. Biol. 6, 49 (2006).

    Google Scholar 

40. Cohen, M. S., Hawkins, M. B., Knox-Hayes, J., Vinton, A. C. & Cruz, A. A laboratory study of host use by the cuckoo catfish Synodontis multipunctatus. Environ. Biol. Fishes 101, 1417–1425 (2018).

    Google Scholar 

41. Title, P. O. et al. The macroevolutionary singularity of snakes. Science 383, 918–923 (2024).

    Google Scholar 

42. Polačik, M., Reichard, M., Smith, C. & Blažek, R. Parasitic cuckoo catfish exploit parental responses to stray offspring. Philos. Trans. R. Soc. B Biol. Sci. 374, 20180412 (2019).

    Google Scholar 

43. Kattan, G. H. Shiny cowbirds follow the ‘shotgun’ strategy of brood parasitism. Anim. Behav. 53, 647–654 (1997).

    Google Scholar 

44. Payne, R. B. The evolution of clutch size and reproductive rates in parasitic cuckoos. Evolution 28, 169–181 (1974).

    Google Scholar 

45. Langmore, N. E. et al. Coevolution with hosts underpins speciation in brood-parasitic cuckoos. Science 384, 1030–1036 (2024).

    Google Scholar 

46. McClelland, S. C. et al. Highly virulent avian brood-parasitic species show elevated embryonic metabolic rates at specific incubation stages compared to less virulent and non-parasitic species. Biol. Lett. 20, 20240411 (2024).

    Google Scholar 

47. Spottiswoode, C. N. & Koorevaar, J. A stab in the dark: chick killing by brood parasitic honeyguides. Biol. Lett. 8, 241–244 (2012).

    Google Scholar 

48. Reichard, M. & Zimmermann, H. Dataset and analytical code for ‘The ecological and developmental foundations of brood parasitism in a catfish’. Figshare. https://doi.org/10.6084/m9.figshare.28646141 (2026).

49. Oksanen, J. vegan: community ecology package. R package version 2, 5–6 (2019).

    Google Scholar 

50. Wang, Y., Naumann, U., Wright, S. T. & Warton, D. I. mvabund– an R package for model-based analysis of multivariate abundance data. Methods Ecol. Evol. 3, 471–474 (2012).

    Google Scholar 

51. Hogsden, K. L. & McHugh, P. A. Preservatives and sample preparation in stable isotope analysis of New Zealand freshwater invertebrates. N. Z. J. Mar. Freshw. Res. 51, 455–464 (2017).

    Google Scholar 

52. Kiljunen, M. et al. A revised model for lipid-normalizing δ¹³ C values from aquatic organisms, with implications for isotope mixing models. J. Appl. Ecol. 43, 1213–1222 (2006).

    Google Scholar 

53. McCutchan, J. H. Jr, Lewis, W. M. Jr, Kendall, C. & McGrath, C. C. Variation in trophic shift for stable isotope ratios of carbon, nitrogen, and sulfur. Oikos 102, 378–390 (2003).

    Google Scholar 

54. Post, D. M. Using Stable Isotopes to estimate trophic position: models, methods, and assumptions. Ecology 83, 703–718 (2002).

    Google Scholar 

55. Martinez Arabizu, P. pairwiseAdonis: Pairwise Multilevel Comparison using Adonis (R package version 0.4, 1, 2020).

56. Dyková, I. et al. Oogenesis, spermatogenesis and spermiation structures in Lake Tanganyika Synodontis species (Mochokidae, Telostei: Siluriformes). J. Vertebr. Biol. 73, 24023 (2024).

57. Brown-Peterson, N. J., Wyanski, D. M., Saborido-Rey, F., Macewicz, B. J. & Lowerre-Barbieri, S. K. A standardized terminology for describing reproductive development in fishes. Mar. Coast. Fish. 3, 52–70 (2011).

    Google Scholar 

58. Sipos, M. J. et al. Evaluation of cGnRH IIa for induction spawning of two ornamental Synodontis species. Aquaculture 511, 734226 (2019).

    Google Scholar 

59. Troscianko, J. & Stevens, M. Image calibration and analysis toolbox – a free software suite for objectively measuring reflectance, colour and pattern. Methods Ecol. Evol. 6, 1320–1331 (2015).

    Google Scholar 

60. Brooks, M. E. glmmTMB} Balances speed and flexibility among packages for zero-inflated generalized linear mixed modeling. R J. 9, 378–400 (2017).

    Google Scholar 

61. Hartig, F. DHARMa: Residual diagnostics for hierarchical (multi-level/mixed) regression models (R Package Version 0.4. 4, 2021).

62. Lenth R. V., Piaskowski J. emmeans: Estimated Marginal Means, aka Least-squares Means (R package version 2.0.1, 2025).

63. Revell, L. Phytools 2.0: an updated R ecosystem for phylogenetic comparative methods (and other things). PeerJ 12, e16505 (2024).

    Google Scholar 

64. Harmon, L. J., Weir, J. T., Brock, C. D., Glor, R. E. & Challenger, W. GEIGER: investigating evolutionary radiations. Bioinformatics 24, 129–131 (2008).

    Google Scholar 

65. Walker, M. & Kimmel, C. A two-color acid-free cartilage and bone stain for zebrafish larvae. Biotech. Histochem. 82, 23–28 (2007).

    Google Scholar 

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