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In vitro larval rearing method of eusocial bumblebee Bombus terrestris for toxicity test

  • Klein, A. M. et al. Importance of pollinators in changing landscapes for world crops. P. R. Soc. B 274, 303–313 (2007).

    Google Scholar 

  • Potts, S. G. et al. Global pollinator declines: trends, impacts and drivers. Trends Ecol. Evol. 25, 345–353 (2010).

    PubMed 

    Google Scholar 

  • Gallai, N., Salles, J. M., Settele, J. & Vaissiere, B. E. Economic valuation of the vulnerability of world agriculture confronted with pollinator decline. Ecol. Econ. 68, 810–821 (2009).

    Google Scholar 

  • Ollerton, J. Pollinator diversity: Distribution, ecological function, and conservation. Annu. Rev. Ecol. Evol. Syst. 48, 353–376 (2017).

    Google Scholar 

  • Biesmeijer, J. C. et al. Parallel declines in pollinators and insect-pollinated plants in Britain and the Netherlands. Science 313, 351–354 (2006).

    ADS 
    CAS 
    PubMed 

    Google Scholar 

  • Kosior, A. et al. The decline of the bumble bees and cuckoo bees (Hymenoptera : Apidae : Bombini) of Western and Central Europe. Oryx 41, 79–88 (2007).

    Google Scholar 

  • Cameron, S. A. et al. Patterns of widespread decline in North American bumble bees. Proc. Natl. Acad. Sci. U. S. A. 108, 662–667 (2011).

    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Cameron, S. A., Lim, H. C., Lozier, J. D., Duennes, M. A. & Thorp, R. Test of the invasive pathogen hypothesis of bumble bee decline in North America. Proc. Natl. Acad. Sci. U. S. A. 113, 4386–4391 (2016).

    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Gill, R. J., Ramos-Rodriguez, O. & Raine, N. E. Combined pesticide exposure severely affects individual- and colony-level traits in bees. Nature 491, 105–108 (2012).

    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Whitehorn, P. R., O’Connor, S., Wackers, F. L. & Goulson, D. Neonicotinoid pesticide reduces bumble bee colony growth and queen production. Science 336, 351–352 (2012).

    ADS 
    CAS 
    PubMed 

    Google Scholar 

  • Stanley, D. A. et al. Neonicotinoid pesticide exposure impairs crop pollination services provided by bumblebees. Nature 528, 548–550 (2015).

    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Baron, G. L., Raine, N. E. & Brown, M. J. F. General and species-specific impacts of a neonicotinoid insecticide on the ovary development and feeding of wild bumblebee queens. P. R. Soc. B 284, 20170123 (2017).

    Google Scholar 

  • Siviter, H., Folly, A. J., Brown, M. J. F. & Leadbeater, E. Individual and combined impacts of sulfoxaflor and Nosema bombi on bumblebee (Bombus terrestris) larval growth. Proc. Biol. Sci. 287, 20200935 (2020).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Blacquière, T., Smagghe, G., van Gestel, C. A. M. & Mommaerts, V. Neonicotinoids in bees: a review on concentrations, side-effects and risk assessment. Ecotoxicology 21, 973–992 (2012).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Richardson, L. L. et al. Secondary metabolites in floral nectar reduce parasite infections in bumblebees. Proc. Biol. Sci. 282, 20142471 (2015).

    PubMed 
    PubMed Central 

    Google Scholar 

  • McAulay, M. K. & Forrest, J. R. K. How do sunflower pollen mixtures affect survival of queenless microcolonies of bumblebees (Bombus impatiens)?. Arthropod Plant Interact. 13, 517–529 (2019).

    Google Scholar 

  • European Food Safety Authority. Guidance on the risk assessment of plant protection products on bees (Apis mellifera, Bombus spp. and solitary bees). EFSA J. 11, 3295 (2013).

  • Besard, L. et al. Compatibility of traditional and novel acaricides with bumblebees (Bombus terrestris): a first laboratory assessment of toxicity and sublethal effects. Pest Manag. Sci. 66, 786–793 (2010).

    CAS 
    PubMed 

    Google Scholar 

  • Elston, C., Thompson, H. M. & Walters, K. F. A. Sub-lethal effects of thiamethoxam, a neonicotinoid pesticide, and propiconazole, a DMI fungicide, on colony initiation in bumblebee (Bombus terrestris) micro-colonies. Apidologie 44, 563–574 (2013).

    CAS 

    Google Scholar 

  • Barbosa, W. F., De Meyer, L., Guedes, R. N. C. & Smagghe, G. Lethal and sublethal effects of azadirachtin on the bumblebee Bombus terrestris (Hymenoptera: Apidae). Ecotoxicology 24, 130–142 (2015).

    CAS 
    PubMed 

    Google Scholar 

  • Dance, C., Botías, C. & Goulson, D. The combined effects of a monotonous diet and exposure to thiamethoxam on the performance of bumblebee micro-colonies. Ecotoxicol. Environ. Saf. 139, 194–201 (2017).

    CAS 
    PubMed 

    Google Scholar 

  • Schmehl, D. R., Tome, H. V. V., Mortensen, A. N., Martins, G. F. & Ellis, J. D. Protocol for the in vitro rearing of honey bee (Apis mellifera L.) workers. J. Apic. Res. 55, 113–129 (2016).

  • Pereboom, J. J. M., Velthuis, H. H. W. & Duchateau, M. J. The organisation of larval feeding in bumblebees (Hymenoptera, Apidae) and its significance to caste differentiation. Insectes Soc. 50, 127–133 (2003).

    Google Scholar 

  • Dorigo, A. S., Rosa-Fontana, A. D., Soares-Lima, H. M., Galaschi-Teixeira, J. S., Nocelli, R. C. F. & Malaspina, O. In Vitro larval rearing protocol for the stingless bee species Melipona scutellaris for toxicological studies. PLoS One 14. https://doi.org/10.1371/journal.pone.0213109 (2019).

  • Botina, L. L. et al. Toxicological assessments of agrochemical effects on stingless bees (Apidae, Meliponini). MethodsX 7, 100906 (2020).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Black, B. C., Hollingworth, R. M., Ahammadsahib, K. I., Kukel, C. D. & Donovan, S. Insecticidal Action and Mitochondrial Uncoupling Activity of AC-303,630 and Related Halogenated Pyrroles. Pestic. Biochem. Physiol. 50, 115–128 (1994).

    CAS 

    Google Scholar 

  • Wakita, T. et al. The discovery of dinotefuran: a novel neonicotinoid. Pest Manag. Sci. 59, 1016–1022 (2003).

    CAS 
    PubMed 

    Google Scholar 

  • Shafiei, M., Moczek, A. P. & Nijhout, H. F. Food availability controls the onset of metamorphosis in the dung beetle Onthophagus taurus (Coleoptera: Scarabaeidae). Physiol. Entomol. 26, 173–180 (2001).

    Google Scholar 

  • Stieper, B. C., Kupershtok, M., Driscoll, M. V. & Shingleton, A. W. Imaginal discs regulate developmental timing in Drosophila melanogaster. Dev. Biol. 321, 18–26 (2008).

    CAS 
    PubMed 

    Google Scholar 

  • Nijhout, H. F. & Williams, C. Control of moulting and metamorphosis in the tobacco hornworm, Manduca sexta (L.): growth of the last-instar larva and the decision to pupate. J. Exp. Biol. 61, 481–491 (1974).

  • Cnaani, J., Robinson, G. E. & Hefetz, A. The critical period for caste determination in Bombus terrestris and its juvenile hormone correlates. J. Comp. Physiol. A 186, 1089–1094 (2000).

    CAS 
    PubMed 

    Google Scholar 

  • Goulson, D. et al. Can alloethism in workers of the bumblebee, Bombus terrestris, be explained in terms of foraging efficiency?. Anim. Behav. 64, 123–130 (2002).

    Google Scholar 

  • Syromyatnikov, M., Nesterova, E., Smirnova, T. & Popov, V. Methylene blue can act as an antidote to pesticide poisoning of bumble bee mitochondria. Sci. Rep. 11, 14710 (2021).

    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Raghavendra, K. et al. Chlorfenapyr: a new insecticide with novel mode of action can control pyrethroid resistant malaria vectors. Malar. J. 10, 16 (2011).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Cao, Y. et al. HPLC/UV analysis of chlorfenapyr residues in cabbage and soil to study the dynamics of different formulations. Sci. Total Environ. 350, 38–46 (2005).

    ADS 
    CAS 
    PubMed 

    Google Scholar 

  • Costa, E. M. et al. Toxicity of insecticides used in the Brazilian melon crop to the honey bee Apis mellifera under laboratory conditions. Apidologie 45, 34–44 (2014).

    CAS 

    Google Scholar 

  • Cresswell, J. E., Robert, F.-X.L., Florance, H. & Smirnoff, N. Clearance of ingested neonicotinoid pesticide (imidacloprid) in honey bees (Apis mellifera) and bumblebees (Bombus terrestris). Pest Manag. Sci. 70, 332–337 (2014).

    CAS 
    PubMed 

    Google Scholar 

  • Czerwinski, M. A. & Sadd, B. M. Detrimental interactions of neonicotinoid pesticide exposure and bumblebee immunity. J Exp Zool A Ecol Integr Physiol 327, 273–283 (2017).

    CAS 
    PubMed 

    Google Scholar 

  • Mobley, M. W. & Gegear, R. J. One size does not fit all: Caste and sex differences in the response of bumblebees (Bombus impatiens) to chronic oral neonicotinoid exposure. PLoS ONE 13, e0200041 (2018).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Simmons, W. R. & Angelini, D. R. Chronic exposure to a neonicotinoid increases expression of antimicrobial peptide genes in the bumblebee Bombus impatiens. Sci. Rep. 7, 44773 (2017).

    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Doublet, V., Labarussias, M., de Miranda, J. R., Moritz, R. F. A. & Paxton, R. J. Bees under stress: sublethal doses of a neonicotinoid pesticide and pathogens interact to elevate honey bee mortality across the life cycle. Environ. Microbiol. 17, 969–983 (2015).

    CAS 
    PubMed 

    Google Scholar 

  • Eiri, D. M., Suwannapong, G., Endler, M. & Nieh, J. C. Nosema ceranae can infect honey bee larvae and reduces subsequent adult longevity. PLoS One 10, (2015).

  • Dai, P., Jack, C. J., Mortensen, A. N. & Ellis, J. D. Acute toxicity of five pesticides to Apis mellifera larvae reared in vitro. Pest Manag. Sci. 73, 2282–2286 (2017).

    CAS 
    PubMed 

    Google Scholar 

  • du Rand, E. E. et al. Proteomic and metabolomic analysis reveals rapid and extensive nicotine detoxification ability in honey bee larvae. Insect Biochem. Mol. Biol. 82, 41–51 (2017).

    PubMed 

    Google Scholar 

  • R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria (2021). R Core Team (2021). URL https://www.R-project.org/.


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