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Optimizing boron and zinc supplementation for cane growth and its residual effect on the ratoon crop


Abstract

Sugarcane (Saccharum officinarum L.), as a long-duration and nutrient-intensive crop, is particularly susceptible to micronutrient depletion, especially under intensive cultivation. Despite the essential roles of zinc (Zn) and boron (B) in plant growth and metabolism, their management is often neglected, and their residual effects on ratoon crops remain underexplored. The present study aims to optimize Zn and B supplementation to enhance yield and quality in plant cane while assessing their carry-over effects on ratoon productivity for improved and sustainable micronutrient management in tropical agroecosystems. Field experiments were conducted at three sites on the College Farm, NAU, Navsari during the winter seasons from 2017 to 18 to 2019–20 for plant cane and from 2018 to 19 to 2020–21 for ratoon cane, to evaluate the direct effects of B and Zn application on plant sugarcane and their residual effects on ratoon sugarcane. The treatments included four levels of boron (0, 1.0, 2.0, and 3.0 kg ha⁻¹) and four levels of zinc (0, 5.0, 7.5, and 10.0 kg ha⁻¹), applied along with the recommended dose of fertilizers. The experiment was laid out in a factorial randomized block design with three replications, and the data were subjected to pooled analysis of variance over the years. Significant individual effects of boron and zinc on sugarcane growth and yield was observed. Millable cane height, weight, and the yield of cane and green trash were significantly higher with a B application of 3 kg ha-1 and a Zn application of 10 kg ha-1. Nutrient application influenced the chemical composition of sugarcane, increasing brix (%), sucrose (%), and commercial cane yield (%), particularly at the same application rates. Nutrient content and uptake in sugarcane, specifically nitrogen (N), phosphorus (P₂O₅), potassium (K₂O), boron (B), and zinc (Zn) increased significantly with the application of boron at 3 kg ha-1 and zinc at 10 kg ha-1. No noticeable interaction effect was observed between B and Zn on the yield and quality parameters of both the sugarcane and its ratoon.

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Data availability

The data supporting this study are available from the corresponding author upon reasonable request.

References

  1. Ranganathan, K. et al. Spatio-temporal distribution of sugarcane shoot borer, Chilo infuscatellus (Lepidoptera: Crambidae) and its associated natural enemy Sturmiopsis inferens (Diptera: Tachinidae) in India. Int. J. Trop. Insect Sci. 43, 277–287 (2023).

    Google Scholar 

  2. Varma, P. K., Kumar, K. V. K., Suresh, M., Raja Kumar, N. R. & Sekhar, V. C. Potentiality of native Pseudomonas spp. In promoting sugarcane seedling growth and red rot (Colletotrichum falcatum Went) management. Int. J. Curr. Microbiol. Appl. Sci. 7, 2855–2863. https://doi.org/10.20546/ijcmas.2018.702.348 (2018).

    Google Scholar 

  3. FAO. FAOSTAT Statistical Database. Food and Agriculture Organization of the United Nations, Rome, Italy. Available online: (2023). https://www.fao.org/faostat/

  4. Kumar, A. & Tiwari, O. K. Socio-economic symptoms of sugarcane growers in meerut district of Western U.P., India. Bull. Environ. Pharmacol. Life Sci. 10, 218–221 (2021).

    Google Scholar 

  5. Directorate of Economics and Statistics, Ministry of Agriculture and Farmers Welfare. Agricultural Statistics Report. Government of India. (2024).

  6. Chakrabarti, S., Dutta, A. & Singh, A. K. Micronutrient status of Indian soils: current challenges and management strategies. J. Soil. Sci. Plant. Nutr. 24, 280–294. https://doi.org/10.1007/s42729-024-01350-5 (2024).

    Google Scholar 

  7. Rana, L. et al. Unlocking potential: the role of zinc fortification combating hidden hunger and enhancing nutritional security. J. Exp. Agric. Int. 46, 625–642. https://doi.org/10.1016/j.eja.2025.127801 (2024).

    Google Scholar 

  8. Rashid, A., Zia, M. & Ahmad, W. Micronutrient Fertilizer Use in Pakistan: Historical Perspective and 4R Nutrient Stewardship (CRC, 2022).

  9. Shukla, A. K., Behera, S. K. & Singh, G. Micronutrient fertilizers in Indian agriculture – product profile, availability, forecast and agronomic effectiveness. Indian J. Fert. 17, 348–360 (2021).

    Google Scholar 

  10. Mousavi, S. M., Sedaghat, A. & Esmaeili, M. Zinc in plants: Biochemical functions and dependent signaling. In Metals and Metalloids in Plant Signaling; Aftab, T. (Ed.), Springer Nature, Cham, Switzerland, pp. 241–263 (2024). https://doi.org/10.1007/978-3-031-59024-5_12

  11. Nandal, V. & Solanki, M. The Zn as a vital micronutrient in plants. J. Microbiol. Biotechnol. Food Sci. 11, e4026 (2021).

    Google Scholar 

  12. Cabot, C. et al. A role for Zn in plant defense against pathogens and herbivores. Front. Plant. Sci. 10, 1171. https://doi.org/10.3389/fpls.2019.01171 (2019).

    Google Scholar 

  13. Zhou, J., Wang, Y., Li, R. & Zhang, J. Foliar application of zinc improves physiological responses and sugar accumulation in sugarcane under Zn-deficient soils. Field Crops Res. 299, 108965. https://doi.org/10.1016/j.fcr.2023.108965 (2023).

    Google Scholar 

  14. Madaan, I. et al. Zinc and plant hormones: an updated review. In Zinc in Plants 193–223 (Elsevier, 2025). https://doi.org/10.1016/B978-0-323-91314-0.00016-8.

    Google Scholar 

  15. Mehdi, F. et al. Factors affecting the production of sugarcane yield and sucrose accumulation: suggested potential biological solutions. Front. Plant. Sci. 15, 1374228. https://doi.org/10.3389/fpls.2024.1374228 (2024).

    Google Scholar 

  16. Tanaka, M. & Fujiwara, T. Physiological roles and transport mechanism of boron: perspective from plants. Eur. J. Physiol. 456, 671–677 (2008).

    Google Scholar 

  17. Tariq, M. & Mott, C. J. B. The significance of Boron in plant nutrition and environment—A review. J. Agron. 6, 1–10 (2007).

    Google Scholar 

  18. Wang, X., Liu, Y. & Wu, X. Boron deficiency affects sugar transport and cell wall integrity in sugarcane. Plant. Physiol. Biochem. 182, 38–45. https://doi.org/10.1016/j.plaphy.2022.04.007 (2022).

    Google Scholar 

  19. Mangrio, N., Kandhro, M. N., Soomro, A. A., Mari, N. & Shah, Z. H. Growth, yield and sucrose percent response of sugarcane to zinc and Boron application. Sarhad J. Agric. 36, 459–469. https://doi.org/10.17582/journal.sja/2020/36.2.459.469 (2020).

    Google Scholar 

  20. Marangoni, F. F. et al. Soluble sources of zinc and Boron on sugarcane yield in Southeast Brazil. Sugar Tech. 21, 917–924. https://doi.org/10.1007/s12355-019-00716-x (2019).

    Google Scholar 

  21. Franco, H. C. J. et al. Sugarcane response to Boron and zinc in southeastern Brazil. Sugar Tech. 13, 86–95. https://doi.org/10.1007/s12355-010-0057-x (2011).

    Google Scholar 

  22. Jackson, M. L. Soil Chemical Analysis (Prentice Hall of India Pvt. Ltd., 1979).

  23. Walkley, A. & Black, I. A. An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid Titration method. Soil. Sci. 37, 29–38. https://doi.org/10.1097/00010694-193401000-00003 (1934).

    Google Scholar 

  24. Subbiah, B. V. & Asija, G. L. A rapid procedure for the Estimation of available nitrogen in soils. Curr. Sci. 25, 259–260 (1956).

    Google Scholar 

  25. Lindsay, W. L. & Norvell, W. A. Development of a DTPA soil test for zinc, iron, manganese and copper. Soil. Sci. Soc. Am. J. 42, 421–428. https://doi.org/10.2136/sssaj1978.03615995004200030009x (1978).

    Google Scholar 

  26. Gupta, U. C. Boron nutrition of crops. Adv. Agron. 31, 273–307. https://doi.org/10.1016/S0065-2113(08)60142-X (1980).

    Google Scholar 

  27. Rao, C. Selection Procedure in Sugarcane Varietal Testing pp. 11–14 (Sugarcane Breeding Institute, 1986).

  28. George, P. Cane Sugar Handbook, 17th ed.; John Wiley & Sons: New York, London, Sydney, Toronto, pp. 124–129 (1963).

  29. Bremner, J. M. Total nitrogen. In Methods of Soil Analysis. Part 2: Chemical and Microbial Properties (ed. Black, C. A.) 1049–1178 (American Society of Agronomy, 1965).

    Google Scholar 

  30. Bingham, F. T. & Boron in In Methods of Soil Analysis, Part 2. 431–448 (eds Page, A. L.) (American Society of Agronomy, 1982).

  31. Panse, V. G. & Sukhatme, P. V. Statistical Methods for Agricultural Workers (Indian Council of Agricultural Research, 1978).

  32. Mishra, A. K. et al. Evaluation of critical limit of Boron in calcareous soil under sugarcane cultivation. Agrica 6, 104–107. https://doi.org/10.5958/2394-448X.2017.00024.4 (2017).

    Google Scholar 

  33. Madhuri, K. V. N., Sarala, N. V., Hemanth Kumar, M., Subba Rao, M. & Giridhar, V. Influence of micronutrients on yield and quality of sugarcane. Sugar Tech. 15, 187–191. https://doi.org/10.1007/s12355-013-0230-7 (2013).

    Google Scholar 

  34. Majeed, A. et al. Balanced use of Zn, Cu, Fe, and B improves the yield and sucrose contents of sugarcane juice cultivated in sandy clay loam soil. Agronomy 12, 696. https://doi.org/10.3390/agronomy12030696 (2022).

    Google Scholar 

  35. Bithy, S. et al. Foliar application of Boron boosts the performance of tropical sugar beet. J. Bangladesh Agril Univ. 18, 537–544. https://doi.org/10.5455/JBAU.121026 (2020).

    Google Scholar 

  36. Abbas, M. S., Dewdar, M. D. H., Gaber, E. I. & El-Aleem, H. A. A. Impact of boron foliar application on quantity and quality traits of sugar beet (Beta vulgaris L.) in Egypt.. Res. J. Pharm. Biol. Chem. Sci 5, 143–151 (2014).

  37. Mellis, E. V. et al. Micronutrientes em cana-de-açúcar: novidade lucrativa. In Congresso Brasileiro de Ciência do Solo (2009).

  38. Espironelo, A., Brasil Sobrinho, M. O. C. & Moraes, R. S. Efeitos do Boro Em cana-de-açúcar cultivada Em Vasos Contendo solo. Bragantia 35, 259–272 (1976).

    Google Scholar 

  39. Andrade, L. A. B., Casagrande, A. A., Vitti, G. C. & Perecin, D. Efeitos Das aplicações de Fritas e de fontes solúveis de boro, Cobre e zinco, via solo, Na cultura de cana-de-açúcar (Saccharum spp.), variedade SP70–1143. STAB. – Açúcar Álcool E Subprodutos. 13, 21–27 (1995).

    Google Scholar 

  40. Mazhar, S. Impact of zinc and Boron application on growth, cane yield and recovery in sugarcane. Int. J. Life Sci. 10, 30–37 (2016).

    Google Scholar 

  41. Shireen, F. et al. Functions and approaches to enhance its availability in plants for sustainable agriculture. Int. J. Mol. Sci. 19, 1856. https://doi.org/10.3390/ijms19061856 (2018).

    Google Scholar 

  42. Li, W. & Johnson, C. E. Relationships among pH, aluminum solubility and aluminum complexation with organic matter in acid forest soils of the Northeastern united States. Geoderma 271, 234–242. https://doi.org/10.1016/j.geoderma.2016.03.020 (2016).

    Google Scholar 

  43. Kumar, N., Rana, L., Kumar, V., Sow, S. & Nanda, G. Zinc fertilization and bud chip transplanting: effects on zinc fractions, uptake and sugarcane productivity in calcareous soils. Eur. J. Agron. 171, 127801. https://doi.org/10.1016/j.eja.2025.127801 (2025).

    Google Scholar 

  44. Wu, W., Fu, W., Alatalo, J. M., Ma, Z. & Bai, Y. Effects of coupling water and fertilizer on agronomic traits, sugar content and yield of sugarcane in Guangxi, China. Agronomy https://doi.org/10.3390/agronomy12020321 (2022).

    Google Scholar 

  45. Chitkala Devi, T. C., Bharathalakshmi, M., Kumari, M. B. G. S. & Naidu, N. V. Effect of sources and levels of phosphorus with zinc on yield and quality of sugarcane. Sugar Tech. 14, 195–198. https://doi.org/10.1007/s12355-012-0144-2 (2012).

    Google Scholar 

  46. Naeem, A. et al. Biofortification of diverse basmati rice cultivars with iodine, selenium, and zinc by individual and cocktail spray of micronutrients. Agronomy 12, 49. https://doi.org/10.3390/agronomy12010049 (2021).

    Google Scholar 

  47. Broadley, M. R., White, P. J., Hammond, J. P., Zelko, I. & Lux, A. Zinc in plants. New. Phytol. 173, 677–702. https://doi.org/10.1111/j.1469-8137.2007.01996.x (2007).

    Google Scholar 

  48. Ghaffar, A. et al. Effect of trench spacing and micronutrients on growth and yield of sugarcane (Saccharum officinarum L). Aust J. Crop Sci. 6, 1–9 (2012).

    Google Scholar 

  49. Nadia, M., Kandhro, M. N., Soomro, A. A., Mari, N. & Shah, Z. U. H. Growth, yield and sucrose percent response of sugarcane to zinc and Boron application. Sarhad J. Agric. 36, 459–469 (2020).

    Google Scholar 

  50. Deshmukh, D. P., Navale, A. M. & Deokar, C. D. Influence of zinc solubilising consortiums on yield parameters of Suru sugarcane. Int. J. Agric. Innov. Res. 8, 83–87 (2019).

    Google Scholar 

  51. Filho, M. C. M. T. et al. Rates and sources of zinc applied in sugarcane grown on sandy soil in Brazil. Afr. J. Agric. Res. 10, 477–484 (2015).

    Google Scholar 

  52. Muhammad, I. et al. Response of sugarcane to different doses of Zn at various growth stages. Pure Appl. Biol. 5, 311–316. https://doi.org/10.19045/bspab.2016.50040 (2016).

    Google Scholar 

  53. Jha, C. K. & Thakur, S. K. Integrated effect of sugarcane trash mulch, Pressmud and Zn nutrition on soil fertility and productivity of sugarcane in calcareous soil. J. Agric. Search. 6, 4–7 (2019).

    Google Scholar 

  54. Cunha, F. N. et al. Productive potential of nitrogen and zinc fertigated sugarcane. Agronomy 10, 1096. https://doi.org/10.3390/agronomy10081096 (2020).

    Google Scholar 

  55. Dhaliwal, S. S. et al. Assessment of optimum mineral zinc fertilizer rate for quantitative and qualitative production of sugarcane in North-Western India. J. Trace Elem. Min. 2, 100021. https://doi.org/10.1016/j.jtemin.2022.100021 (2022).

    Google Scholar 

  56. Silva, M. A. et al. P. Sugarcane productivity as a function of zinc dose and application method. Agriculture 12, 1843. https://doi.org/10.3390/agriculture12111843 (2022).

    Google Scholar 

  57. Alvarez, R., Wutke, C. P. A., Arruda, H. V. & Godoy Júnior, G. Adubação Da cana-de-açúcar. XV. Experimentos com micronutrientes Nas regiões Canavieiras do Estado de São Paulo. Bragantia 31, 19–25 (1979).

    Google Scholar 

  58. Marinho, M. F. & Albuquerque, G. A. C. Efeitos do Cobre e do Zinco Na produção de cana-de-açúcar Em solos de tabuleiros de Alagoas. Brasil Açucareiro. 98, 41–50 (1981).

    Google Scholar 

  59. Crusciol, C. A. C. et al. Filter cake as a long-standing source of micronutrients for sugarcane. J. Soil. Sci. Plant. Nutr. 21, 813–823. https://doi.org/10.1007/s42729-020-00403-x (2021).

    Google Scholar 

  60. Pawar, M. W., Joshi, S. S. & Amodkar, V. T. Effect of foliar application of phosphorus and micronutrients on enzyme activities and juice quality in sugarcane. Sugar Tech. 5, 161–165. https://doi.org/10.1007/BF02943628 (2003).

    Google Scholar 

  61. Thangavelu, S. Zinc and sugarcane production. Indian Sugar. 57, 39–46 (2007).

    Google Scholar 

  62. Dhanasekaran, K. & Bhuvaneswari, R. Effect of zinc and iron humate application on the yield and quality of sugarcane. Indian Sugar. 53, 907–912 (2004).

    Google Scholar 

  63. Abd El-Mageed, T. A., Rady, M. O. A., Semida, W. M., Shaaban, A. & Mekdad, A. A. A. Exogenous micronutrients modulate morphophysiological attributes, yield, and sugar quality in two salt-stressed sugar beet cultivars. J. Soil. Sci. Plant. Nutr. 21, 1421–1436. https://doi.org/10.1007/s42729-021-00450-y (2021).

    Google Scholar 

  64. Ghaffar, A., Ehsanullah, N. A. & Khan, S. H. Influence of zinc and iron on yield and quality of sugarcane planted under various trench spacings. Pak J. Agric. Sci. 48, 25–33 (2011).

    Google Scholar 

  65. Xian, X. et al. Effects of combined application of phosphorus and zinc on growth and physiological characteristics of Apple rootstock M9-T337 seedlings (Malus domestica Borkh). BMC Plant. Biol. 24, 998. https://doi.org/10.1186/s12870-024-05724-y (2024).

    Google Scholar 

  66. Nayyer, V. K., Singh, S. P. & Takkar, P. N. Response of sugarcane to zinc and iron sources. J. Res. Punjab Agric. Univ. 21, 134–136 (1989).

    Google Scholar 

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Acknowledgements

Authors would like to thank the Central Instrumentation Laboratory and Department of Soil Science, Navsari Agricultural University for the structural support to conduct this study.

Funding

The research was funded by the Department of Soil Science, N. M. College of Agriculture, Navsari Agricultural University, Navsari 396450.

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Deepasree Ammamkuzhiyil.

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Zinzala, V.J., Patel, J.V., Tripathi, S. et al. Optimizing boron and zinc supplementation for cane growth and its residual effect on the ratoon crop.
Sci Rep (2026). https://doi.org/10.1038/s41598-025-29338-y

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  • DOI: https://doi.org/10.1038/s41598-025-29338-y

Keywords

  • Boron fertilization
  • Micronutrient management
  • Nutrient use efficiency
  • Ratoon crop
  • Residual effect
  • Soil fertility
  • Sugarcane productivity
  • Zinc application


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