
Ghazoul, J. Pollen and seed dispersal among dispersed plants. Biol. Rev. 80, 413 (2005).
Jordano, P. What is long-distance dispersal? And a taxonomy of dispersal events. J. Ecol. 105, 75–84 (2017).
Vekemans, X. & Hardy, O. J. New insights from fine-scale spatial genetic structure analyses in plant populations. Mol. Ecol. 13, 921–935 (2004).
Wright, S. Isolation by distance. Genetics 28, 114–138 (1943).
Bohonak, A. J. Dispersal, Gene Flow, and Population Structure. Q. Rev. Biol. 74, 21–45 (1999).
Kalisz, S., Nason, J. D., Hanzawa, F. M. & Tonsor, S. J. Spatial population genetic structure in Trillium grandiflorum: the roles of dispersal, mating, history and selection. Evolution 55, 1560–1568 (2001).
Pardini, E. A. & Hamrick, J. L. Inferring recruitment history from spatial genetic structure within populations of the colonizing tree Albizia julibrissin (Fabaceae). Mol. Ecol. 17, 2865–2879 (2008).
Jacquemyn, H., Brys, R., Vandepitte, K., Honnay, O. & Roldán-Ruiz, I. Fine-scale genetic structure of life history stages in the food-deceptive orchid Orchis purpurea. Mol. Ecol. 15, 2801–2808 (2006).
Jones, F. A. & Hubbell, S. P. Demographic spatial genetic structure of the Neotropical tree, Jacaranda copaia. Mol. Ecol. 15, 3205–3217 (2006).
Choo, J., Juenger, T. E. & Simpson, B. B. Consequences of frugivore-mediated seed dispersal for the spatial and genetic structures of a neotropical palm. Mol. Ecol. 21, 1019–1031 (2012).
Nazareno, A. G., Alzate-Marin, A. L. & Pereira, R. A. S. Dioecy, more than monoecy, affects plant spatial genetic structure: the case study of Ficus. Ecol. Evol. 3(10), 3495–3508 (2013).
Gelmi-Candusso, T. A., Heymann, E. W. & Heer, K. Effects of zoochory on the spatial genetic structure of plant populations. Mol. Ecol. 26, 5896–5910 (2017).
Janzen, D. H. Herbivores and the Number of Tree Species in Tropical Forests. Am. Nat. 104, 501–528 (1970).
Connell, J. H. On the role of natural enemies in preventing competitive exclusion in some marine animals and in rain forest trees in Dy namics of Populations. (eds. Den Boer, P. J. & Gradwell, G. R.) 298–312 (Centre for Agricultural Publishing and Documentation, 1971).
Comita, L. S. et al. Testing predictions of the Janzen–Connell hypothesis: a meta‐analysis of experimental evidence for distance‐ and density‐dependent seed and seedling survival. J. Ecol. 102, 845–856 (2014).
Bawa, K. S., Opler, P. A., Rica, D. C. & Rica, C. Dioecism in Tropical Forest Trees. Evolution 29, 167–179 (1975).
Bawa, K. S., Perry, D. R. & Beach, J. H. Reproductive Biology of Tropical Lowland Rain Forest Trees. I. Sexual Systems and Incompatibility Mechanisms. Am. J. Bot. 72, 331 (1985).
Ibarra-Manriquez, G. & Oyama, K. Ecological Correlates of Reproductive Traits of Mexican Rain Forest Trees. Am. J. Bot. 79, 383 (1992).
Renner, S. S. & Ricklefs, R. E. Dioecy and its correlates in the flowering plants. Am. J. Bot. 82, 596–606 (1995).
Zhou, H. P. & Chen, J. Spatial genetic structure in an understorey dioecious fig species: the roles of seed rain, seed and pollen-mediated gene flow, and local selection. J. Ecol. 98, 1168–1177 (2010).
Riba‐Hernández, P., Segura, J. L. & Muñoz‐Valverde, J. Female fruit production depends on female flower production and crown size rather than male density in a continuous population of a tropical dioecious tree (Virola surinamensis). Am. J. Bot. 103, 1990–1999 (2016).
Degen, B. & Sebbenn, A. M. Genetics and Tropical Forests in Tropical Forestry Handbook (eds. Köhl, M. & Pancel, L) (Springer, 2014).
Murawski, D. & Hamrick, J. The effect of the density of flowering individuals on the mating systems of nine tropical tree species. Heredity 67, 167–174 (1991).
Bianchi, F. J. J. A. & Cunningham, S. A. Unravelling the role of mate density and sex ratio in competition for pollen. Oikos 121, 219–227 (2012).
Castilla, A. R. et al. Adding landscape genetics and individual traits to the ecosystem function paradigm reveals the importance of species functional breadth. Proc Natl Acad Sci USA 114, 12761–12766 (2017).
Grant, E. L. et al. Short distance pollen dispersal and low genetic diversity in a subcanopy tropical rainforest tree, Fontainea picrosperma (Euphorbiaceae). Heredity 123, 503–516 (2019).
Barrett, S. C. H., Yakimowski, S. B., Field, D. L. & Pickup, M. Ecological genetics of sex ratios in plant populations. Philos. T. R. Soc. B. 365, 2549–57 (2010).
Lloyd, D. G. & Webb, C. J. Secondary sex characters in plants. Bot. Rev. 43, 177–216 (1977).
Obeso, J. R., Alvarez-Santullano, M. & Retuerto, R. Sex ratios, size distributions, and sexual dimorphism in the dioecious tree Ilex aquifolium (Aquifoliaceae). Am. J. Bot. 85, 1602–1608 (1998).
Wilson, A. J. & Nussey, D. H. What is individual quality? An evolutionary perspective. Trends Ecol. Evol. 25(4), 207–214 (2010).
Meagher, T. R. & Thompson, E. Analysis of Parentage for Naturally Established Seedlings of Chamaelirium Luteum (Liliaceae). Ecology 68, 803–812 (1987).
Primack, R. B. & Kang, H. Measuring fitness and natural selection in wild plant populations. Annu. Rev. Ecol. Syst. 20, 367–396 (1987).
Kingsolver, J. G., Diamond, S. E., Siepielski, A. M. & Stephanie, M. C. Synthetic analyses of phenotypic selection in natural populations: lessons, limitations and future directions. Evol. Ecol. 26, 1101 (2012).
Campbell, D. R., Brody, A. K., Price, M. V., Waser, N. M. & Aldridge, G. Is Plant Fitness Proportional to Seed Set? An Experiment and a Spatial Model. Am. Nat. 190(6), 818–827 (2017).
Sant’Anna, C. et al. Realized pollen and seed dispersal within a continuous population of the dioecious coniferous Brazilian pine [Araucaria angustifolia (Bertol.) Kuntze]. Conserv. Genet. 14(3), 601–613 (2013).
Opler, P. A. & Bawa, K. S. Sex ratios in tropical forest trees. Evolution 32, 812–821 (1978).
Bullock, S. H. & Bawa, K. S. Sexual Dimorphism and the Annual Flowering Pattern in Jacaratia Dolichaula (D. Smith) Woodson (Caricaceae) in a Costa Rican Rain Forest. Ecology 62, 1494–1504 (1981).
Allen, G. A. & Antos, J. A. Sex Ratio Variation in the Dioecious Shrub Oemeleria cerasiformis. Am. Nat. 141, 537–553 (1993).
Nicotra, A. B. Sex ratio variation and spatial distribution of Siparuna grandiflora, a tropical dioecious shrub. Oecologia 115, 102–113 (1998).
Thomas, S. C., LaFrankie, J. V., Thomas, A. S. C. & LaFrankie, J. V. Sex, size and interyear variation in flowering among dioecious trees of the malayan rain forest. Ecology 74, 1529–1537 (1993).
García, M. & Antor, R. Sex Ratio and Sexual Dimorphism in the Dioecious Borderea pyrenaica (Dioscoreaceae). Oecologia 101(1), 59–67 (1995).
Hamrick, J. L., Godt, M. J. W. & Sherman-Broyles, S. L. Factors influencing levels of genetic diversity in woody plant species. New Forest 6, 95–124 (1992).
Hamrick, J. L. Response of forest trees to global environmental changes. Forest Ecol. Manag. 197, 323–335 (2004).
Dick, C. W., Hardy, O. J., Jones, F. A. & Petit, R. J. Spatial Scales of Pollen and Seed-Mediated Gene Flow in Tropical Rain Forest Trees. Trop. Plant Biol. 1, 20–33 (2008).
Hardesty, B. D., Dick, C. W., Kremer, A., Hubbell, S. & Bermingham, E. Spatial genetic structure of Simarouba amara Aubl. (Simaroubaceae), a dioecious, animal-dispersed Neotropical tree, on Barro Colorado Island, Panama. Heredity 95, 290–297 (2005).
Dev, S. A., Kjellberg, F., Hossaert-McKey, M. & Borges, R. M. Fine-scale Population Genetic Structure of Two Dioecious Indian Keystone Species, Ficus hispida and Ficus exasperata (Moraceae). Biotropica 43, 309–316 (2011).
Loiselle, B. A., Sork, V. L., Nason, J. & Graham C. Spatial genetic structure of a tropical understory shrub, Psychotria officinalis (Rubiaceae). Am. J. Bot. 82, 1420–1425 (1995).
Chung, M. Y., Epperson, B. K. & Chung, M.G. Genetic structure of age classes in Camellia japonica (Theaceae). Evolution 57, 62–73 (2003).
Epperson, B. K. & Alvarez-Buylla, E. R. Limited seed dispersal and genetic structure in life stages of cecropia obtusifolia. Evolution 51, 275–282 (1997).
Kitamura, K., Nakanishi, A., Lian, C. & Goto, S. Distinctions in Fine-Scale Spatial Genetic Structure Between Growth Stages of Picea jezoensis Carr. Front. Genet. 9, 490 (2018).
Schroeder, J. W., Trana, H. T. & Dick, C. W. Fine scale spatial genetic structure in Pouteria reticulata (Engl.) Eyma (Sapotaceae), a dioecious, vertebrate dispersed tropical rain forest tree species. Glob. Ecol. Conserv. 1, 43–49 (2014).
Vieira, F. A., Fajardo, C. G., Souza, A. M., Reis, C. A. F. & Carvalho, D. Fine‐scale genetic dynamics of a dominant neotropical tree in the threatened Brazilian Atlantic Rainforest. Tree Genet. Genomes 8, 1191–1201 (2012).
Chung, M. G. & Epperson, B. K. Clonal and spatial genetic structure in Eurya emarginata (Theaceae). Heredity 84, 170–177 (2000).
Trapnell, D. W., Schmidt, J. P. & Hamrick, J. L. Spatial genetic structure of the Southeastern North American endemic, Ceratiola ericoides (Empetraceae). J. Hered. 99, 604–609 (2008).
Wang, R. et al. Spatial genetic structure and restricted gene flow in a functionally dioecious fig Ficus pumila L. pumila (Moraceae). Popul. Ecol. 51, 307–315 (2009).
Gaino, A. P. S. C. et al. Understanding the effects of isolation on seed and pollen flow, spatial genetic structure and effective population size of the dioecious tropical tree Myracrodruon urundeuva. Conserv. Genet. 11, 1631–1643 (2010).
Mandujano, S., Gallina, S. & Bullock, S. H. Frugivory and dispersal of Spondias purpurea (Anacardiaceae) in a tropical deciduous forest in México. Rev. Biol. Trop. 42, 107–114 (1994).
Heilbuth, J. C., Ilves, K. L. & Otto, S. P. The consequences of dioecy for seed dispersal: modeling the seed‐shadow handicap. Evolution 55, 880–888 (2001).
Hardesty, B. D., Hubbell, S. P. & Bermingham, E. Genetic evidence of frequent long‐distance recruitment in a vertebrate‐dispersed tree. Ecol. Lett. 9, 516–525 (2006).
Wang, B. C., Sork, V. L., Leong, M. T. & Smith, T. B. Hunting of Mammals Reduces Seed Removal and Dispersal of the Afrotropical Tree Antrocaryon klaineanum (Anacardiaceae). Biotropica 39(3), 340–347 (2007).
Sezen, U. U., Chazdon, R. L. & Holsinger, K. E. Proximity is not a proxy for parentage in an animal- dispersed Neotropical canopy palm. Proc. R. Soc. B. 276, 2037–2044 (2009).
Solís-Hernández, W. & Fuchs, E. J. Effective gene flow patterns across a fragmented landscape in southern Costa Rica for Symphonia globulifera (Clusiaceae): a species with mobile seed and pollen dispersers. Rev. Biol. Trop. 67(2), 95–111 (2019).
Howe, H. F. Bird Activity and Seed Dispersal of a Tropical Wet Forest Tree. Ecology 58, 539–550 (1977).
Fuchs, E. J. & Hamrick, J. L. Spatial genetic structure within size classes of the endangered tropical tree Guaiacum sanctum (Zygophyllaceae). Am. J. Bot. 97, 1200–1207 (2010).
Dow, B. D. & Ashley, M. V. Microsatellite analysis of seed dispersal and parentage of saplings in bur oak, Quercus macrocarpa. Mol. Ecol. 5, 615–627 (1996).
Streiff, R. et al. Pollen dispersal inferred from paternity analysis in a mixed oak stand of Quercus robur L. and Q. petraea (Matt.) Liebl. Mol. Ecol. 8, 831–841 (1999).
Nathan, R. & Muller-Landau, H. C. Spatial patterns of seed dispersal, their determinants and consequences for recruitment. Trends Ecol. Evol. 15(7), 278–285 (2000).
Godoy, J. A. & Jordano, P. Seed dispersal by animals: exact identification of source trees with endocarp DNA microsatellites. Mol. Ecol. 10, 2275–2283 (2001).
Dick, C. W., Etchelecu, G. & Austerlitz, F. Pollen dispersal of tropical trees (Dinizia excelsa: Fabaceae) by native insects and African honeybees in pristine and fragmented Amazonian rainforest. Mol. Ecol. 12, 753–764 (2003).
Méndez-Toribio, M., Pierro, A. M. G.-D., Quesada, M. & Benítez-Malvido, J. Regeneration beneath a dioecious tree species in a Mexican tropical dry forest. J. Trop. Ecol. 30, 265–268 (2014).
Sugiyama, A., Comita, L., Masaki, T., Condit, R. & Hubbell, S. Resolving the paradox of clumped seed dispersal: positive density and distance dependence in a bat-dispersed species. Ecology 99(1), 2583–2591 (2019).
Zhu, Y. et al. Density-dependent survival varies with species life-history strategy in a tropical forest. Ecol. Lett. 21(4), 506–515 (2018).
Steinitz, O., Troupin, D., Vendramin, G. G. & Nathan, R. Genetic evidence for a Janzen–Connell recruitment pattern in reproductive offspring of Pinus halepensis trees. Mol. Ecol. 20, 4152–4164 (2011).
Berens, D. G. et al. Changes of effective gene dispersal distances by pollen and seeds across successive life stages in a tropical tree. Oikos 122, 1616–1625 (2013).
Younginger, B. S., Sirová, D., Cruzan, M. B. & Ballhorn, D. J. Is biomass a reliable estimate of plant fitness? Appl. Plant Sci. 5, 1600094, https://doi.org/10.3732/apps.1600094 (2017).
Stacy, E. A. et al. Pollen Dispersal in Low-Density Populations of Three Neotropical Tree Species. Am. Nat. 148(2), 275–298 (1996).
Ward, M., Dick, C. W., Gribel, R. & Lowe, A. J. To self, or not to self… A review of outcrossing and pollen-mediated gene flow in neotropical trees. Heredity 95, 246–254 (2005).
Bawa, K. S. Evolution of dioecy in flowering plants. Ann. Rev. Ecol. Syst. 11, 15–39 (1980).
Bruijning, M. et al. Surviving in a Cosexual World: A Cost-Benefit Analysis of Dioecy in Tropical Trees. Am. Nat. 189(3), 297–314 (2017).
Harms, K. E., Wright, J. S., Calderón, O., Hernández, A. & Herre, E. A. Pervasive density-dependent recruitment enchances seedling diversity in a tropical forest. Nature 404, 493–495 (2000).
Lebrija‐Trejos, E., Reich, P. B., Hernández, A. & Wright, S. J. Species with greater seed mass are more tolerant of conspecific neighbours: a key driver of early survival and future abundances in a tropical forest. Ecol. Lett. 19, 1071–1080 (2016).
Miller, A. J. & Knouft, J. H. GIS-based characterization of the geographic distributions of wild and cultivated populations of the Mesoamerican fruit tree Spondias purpurea (Anacardiaceae). Am. J. Bot. 93, 1757–1767 (2006).
Miller, A. & Schaal, B. Domestication of a Mesoamerican cultivated fruit tree, Spondias purpurea. Proc. Natl. Acad. Sci. USA 102, 12801–12806 (2005).
Mitchell, J. D. & Daly, D. C. A revision of Spondias L. (Anacardiaceae) in the Neotropics. PhytoKeys 55, 1–92 (2015).
Wilson, K. & Hardy, I. C. W. Statistical analysis of sex ratios: an introduction. (ed. Hardy, I. C. W.) Sex Ratios. 48–92 (Cambridge University Press, 2002).
Hervé, M. RVAideMemoire: testing and plotting procedures for biostatistics. R package version 0.9-69-3, https://CRAN.R-project.org/package=RVAideMemoire Computer software (2018).
R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria (2018).
Doyle, J. J. & Doyle, J. L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19, 11–15 (1987).
Cristóbal-Pérez, E. J., Fuchs, E. J., Harvey, N. & Quesada, M. Isolation and characterization of microsatellites loci in Spondias purpurea (Anacardiaceae) and cross amplification in congeneric species. Mol. Biol. Rep. 46(5), 5581–5585 (2019).
Excoffier, L. & Lischer, H. E. L. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol. Ecol. Resour. 10, 564–567 (2010).
Goudet, J. H. a package for r to compute and test hierarchical F‐statistics. Mol. Ecol. Res. 5, 184–186 (2005).
Hardy, O. J. & Vekemans, X. Spagedi: a versatile computer program to analyse spatial genetic structure at the individual or population levels. Mol. Ecol. Res. 2, 618–620 (2002).
Besag, J. Contribution to the discussion of Dr. Ripley’s paper. J. Roy. Stat. Soc. B 39, 193–195 (1977).
Goreaud, F. & Pélissier, R. Avoiding misinterpretation of biotic interactions with the intertype K12-function: population independence vs. random labeling hypotheses. J. Veg. Sci. 14, 681–692 (2003).
Baddeley, A. & Turner, R. Spatstat: an R package for analyzing spatial point patterns. J. Stat. Softw. 12, 1–42 (2005).
Jones, O. R. & Wang, J. COLONY: a program for parentage and sibship inference from multilocus genotype data. Mol. Ecol. Res. 10, 551–555 (2010).
Kalinowski, S. T., Taper, M. L. & Marshall, T. C. Revising how the computer program Cervus accommodates genotyping error increases success in paternity assignment. Mol. Ecol. 16, 1099–1106 (2007).
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