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Brucellosis in wildlife in Africa: a systematic review and meta-analysis

  • 1.

    Bengis, R. G. A revue of bovine Brucellosis in free-ranging African wildlife. in Proceedings of the ARC-Onderstepoort, OIE International Congress with WHO-Cosponsorship on anthrax, brucellosis, CBPP, clostridial and mycobacterial diseases : Berg-en-Dal, Kruger National Park, South Africa 178–183 (Onderstepoort Veterinary Inst, 1998).

  • 2.

    Kaliner, G., Staak, C., Kalinerj, G. & Staaklu, C. A case of orchitis caused by Brucella abortus in the African buffalo. J. Wildl. Dis. 9, 251–253 (1973).

    Article 

    Google Scholar 

  • 3.

    Schiemann, B. & Staak, C. Brucella melitensis in impala (Aepyceros melampus). Vet. Rec. 88, 344–344 (1971).

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 4.

    Ndengu, M. et al. Seroprevalence of brucellosis in cattle and selected wildlife species at selected livestock/wildlife interface areas of the Gonarezhou National Park Zimbabwe. Prev. Vet. Med. 146, 158–165 (2017).

    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 5.

    Rollinson, D. H. L. Brucella agglutinins in East African game animals. Vet. Rec. 74, 904 (1962).

    Google Scholar 

  • 6.

    De Vos, V. & Van Niekerk, C. A. W. Brucellosis in the Kruger National Park. J. S. Afr. Vet. Assoc. 40, 331–334 (1969).

    Google Scholar 

  • 7.

    Sachs, R. & Staak, C. Evidence of brucellosis in antelope in the Serengeti. Vet. Record 79, 857–856 (1966).

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 8.

    El-Tras, W. F., Tayel, A. A., Eltholth, M. M. & Guitian, J. Brucella infection in fresh water fish : Evidence for natural infection of Nile catfish, Clarias gariepinus, with Brucella melitensis. Vet. Microbiol. 141, 321–325 (2010).

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 9.

    Lane, E. P. et al. A systematic health assessment of Indian ocean bottlenose (Tursiops aduncus) and indo-pacific humpback (Sousa plumbea) dolphins incidentally caught in shark nets off the KwaZulu-Natal coast South Africa. PLoS ONE 9, e107038 (2014).

    ADS 
    PubMed 
    PubMed Central 
    Article 
    CAS 

    Google Scholar 

  • 10.

    Salem, A. A., Hamed, O. M. & Abd-Elkarim, A. M. Studies on some Brucella carriers in Egypt. Assiut Vet Med J 1, 181–187 (1974).

    Google Scholar 

  • 11.

    Condy, J. B. The status of disease in Rhodesian wildlife. Rhod. Sci. News 2, 96–99 (1968).

    Google Scholar 

  • 12.

    Condy, J. B. & Vickers, D. B. The isolation of Brucella abortus from a waterbuck (Kobus ellipsiprymnus). Vet. Rec. 85, 200 (1969).

    Article 

    Google Scholar 

  • 13.

    Bell, L. M., Hayles, L. B. & Chanda, A. B. Evidence of reservoir hosts of Brucella melitensis. Med. J. Zambia 10, 152–153 (1976).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • 14.

    Gradwell, D. V., Schutte, A. P., van Niekerk, C. A. & Roux, D. J. The isolation of Brucella abortus biotype I from African buffalo in the Kruger National Park. J. S. Afr. Vet. Assoc. 48, 41–43 (1977).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • 15.

    Karesh, W. B. et al. Health evaluation of five sympatric duiker species (Cephalophus spp.). J. Zool. Wildl. Med. 26, 485–502 (1995).

    Google Scholar 

  • 16.

    Fick, S. E. & Hijmans, R. J. WorldClim 2: New 1-km spatial resolution climate surfaces for global land areas. Int. J. Climatol. 37, 4302–4315 (2017).

    Article 

    Google Scholar 

  • 17.

    Bengis, R. G. & Erasmus, J. M. Wildlife diseases in South Africa: A review. Rev. Sci. Tech. Off. Int. des Epizoot. 7, 807–821 (1988).

    Article 

    Google Scholar 

  • 18.

    Durrheim, D. N. et al. Safety of travel in South Africa: The Kruger National Park. J. Travel Med. 8, 176–191 (2006).

    Article 

    Google Scholar 

  • 19.

    Eisenberg, T. et al. Isolation of potentially novel Brucella spp. from frogs. Appl. Environ. Microbiol. 78, 3753–3755 (2012).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • 20.

    Hoogstral, H., Kaiser, M. N., Traylor, M. A., Guindy, E. & Gaber, S. Ticks (Ixodidae) on birds migrating from Europe and Asia to Africa 1959–61. Bull. World Health Organ. 28, 235–262 (1963).

    Google Scholar 

  • 21.

    Michel, A. L. A. L. & Bengis, R. G. R. G. The African buffalo: A villain for inter-species spread of infectious diseases in southern Africa. Onderstepoort. J. Vet. Res. 79, 5 (2012).

    Article 

    Google Scholar 

  • 22.

    Monroe, B. P. et al. Collection and utilization of animal carcasses associated with zoonotic disease in Tshuapa district, the democratic republic of the Congo, 2012. J. Wildl. Dis. 51, 734–738 (2015).

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 23.

    Wolhuter, J., Bengis, R. G., Reilly, B. K. & Cross, P. C. Clinical demodicosis in African buffalo (Syncerus caffer) in the Kruger National Park. J. Wildl. Dis. 45, 2 (2009).

    Article 

    Google Scholar 

  • 24.

    Worthington, R. W. & Bigalke, R. D. A review of the infectious diseases of African wild ruminants. Onderstepoort. J. Vet. Res. 68, 291–323 (2001).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • 25.

    Mühldorfer, K. et al. The role of ‘atypical’ Brucella in amphibians: are we facing novel emerging pathogens?. J. Appl. Microbiol. 122, 40–53 (2017).

    PubMed 
    Article 
    CAS 
    PubMed Central 

    Google Scholar 

  • 26.

    Ducrotoy, M. et al. Brucellosis in Sub-Saharan Africa: Current challenges for management, diagnosis and control. Acta Trop. 165, 179–193 (2017).

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 27.

    Munagandu, et al. Disease constraints for utilization of the African buffalo (Syncerus caffer) on game ranches in Zambia. Jpn. J. Vet. Res. 54, 3–13 (2006).

    Google Scholar 

  • 28.

    Munyua, P. et al. Prioritization of zoonotic diseases in Kenya, 2015. PLoS ONE 11, e0161576 (2016).

    PubMed 
    PubMed Central 
    Article 
    CAS 

    Google Scholar 

  • 29.

    Conrad, P. A., Meek, L. A. & Dumit, J. Operationalizing a One Health approach to global health challenges. Comp. Immunol. Microbiol. Infect. Dis. 36, 211–216 (2013).

    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 30.

    Bekker, J. L., Hoffman, L. C. & Jooste, P. J. Wildlife-associated zoonotic diseases in some southern African countries in relation to game meat safety: A review. Onderstepoort. J. Vet. Res. 79, 12 (2012).

    Article 

    Google Scholar 

  • 31.

    Muma, J. B. et al. The contribution of veterinary medicine to public health and poverty reduction in developing countries. Vet. Ital. 50, 117–129 (2014).

    PubMed 
    PubMed Central 

    Google Scholar 

  • 32.

    Mugizi, D. R. et al. Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda. 2015, (2015).

  • 33.

    Mathew, C. et al. First isolation, identification, phenotypic and genotypic characterization of Brucella abortus biovar 3 from dairy cattle in Tanzania. BMC Vet. Res. 11, 2 (2015).

    Article 

    Google Scholar 

  • 34.

    Meyer, M. E. & Morgan, W. J. B. Designation of neotype strains and of biotype reference strains for species of the genus Brucella Meyer and Shaw. Int. J. Syst. Bacteriol. 23, 135–141 (1973).

    Article 

    Google Scholar 

  • 35.

    National Academies of Sciences, Engineering, and M. Revisiting brucellosis in the greater yellowstone area. Revisiting Brucellosis in the Greater Yellowstone Area (National Academies Press, 2017). doi:https://doi.org/10.17226/24750

  • 36.

    Muma, J. B. et al. Brucella seroprevalence of the Kafue lechwe (Kobus leche kafuensis) and Black lechwe (Kobus leche smithemani): Exposure associated to contact with cattle. Prev. Vet. Med. 100, 256–260 (2011).

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 37.

    Gorsich, E. E., Ezenwa, V. O., Cross, P. C., Bengis, R. G. & Jolles, A. E. Context-dependent survival, fecundity and predicted population-level consequences of brucellosis in African buffalo. J. Anim. Ecol. 84, 999–1009 (2015).

    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 38.

    Hoogstraal, H., Kaiser, M. N., Traylor, M. A., Gaber, S. & Guindy, E. Ticks (Ixodoidea) on birds migrating from Africa to Europe and Asia. Bull. World Health Organ. 24, 197–212 (1961).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • 39.

    Alexander, K. A. et al. Buffalo, bush meat, and the zoonotic threat of brucellosis in Botswana. PLoS ONE 7, e32842 (2012).

    ADS 
    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • 40.

    Munn, Z., Moola, S., Riitano, D. & Lisy, K. The development of a critical appraisal tool for use in systematic reviews addressing questions of prevalence. Int. J. Heal. Policy Manag. 3, 123–128 (2014).

    Article 

    Google Scholar 

  • 41.

    Madsen, M. et al. Serologic survey of Zimbabwean wildlife for brucellosis. J. Zoo. Wildl. Med. 26, 240–245 (1995).

    Google Scholar 

  • 42.

    Roberts, M. G. & Heesterbeek, J. A. P. Quantifying the dilution effect for models in ecological epidemiology. J. R. Soc. Interface 15, 2 (2018).

    Article 

    Google Scholar 

  • 43.

    Viana, M. et al. Assembling evidence for identifying reservoirs of infection. Trends Ecol. Evol. 29, 270–279 (2014).

    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • 44.

    Souley Kouato, B. et al. Spatio-temporal patterns of foot-and-mouth disease transmission in cattle between 2007 and 2015 and quantitative assessment of the economic impact of the disease in Niger. Transbound Emerg. Dis. 65, 1049–1066 (2018).

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 45.

    Godfroid, J., Nielsen, K. & Saegerman, C. Diagnosis of brucellosis in livestock and wildlife. Croat Med. J. 51, 296–305 (2010).

    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • 46.

    Hartling, L. et al. Grey literature in systematic reviews : a cross-sectional study of the contribution of non-English reports, unpublished studies and dissertations to the results of meta- analyses in child-relevant reviews. 1–11 (2017). doi:https://doi.org/10.1186/s12874-017-0347-z

  • 47.

    Condy, J. B. & Vickers, D. B. Brucellosis in Rhodesian wildlife. J. S. Afr. Vet. Assoc. 43, 175–179 (1972).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • 48.

    Erume, J. et al. Serological and molecular investigation for brucellosis in swine in selected districts of Uganda. Trop. Anim. Health Prod. 48, 1147–1155 (2016).

    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • 49.

    Godfroid, J., Beckmen, K. & Helena Nymo, I. Removal of lipid from serum increases coherence between brucellosis rapid agglutination test and enzyme-linked immunosorbent assay in bears in Alaska, USA. J. Wildl. Dis. 52, 912–915 (2016).

    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 50.

    Matope, G., Bhebhe, E., Muma, J. B. B., Lund, A. & Skjerve, E. Herd-level factors for Brucella seropositivity in cattle reared in smallholder dairy farms of Zimbabwe. Prev. Vet. Med. 94, 213–221 (2010).

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 51.

    Mwebe, R., Nakavuma, J. & Moriyón, I. Brucellosis seroprevalence in livestock in Uganda from 1998 to 2008: a retrospective study. Trop. Anim. Health Prod. 43, 603–608 (2011).

    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 52.

    Aune, K., Rhyan, J. C., Russell, R., Roffe, T. J. & Corso, B. Environmental persistence of Brucella abortus in the Greater Yellowstone Area. J. Wildl. Manag. 76, 253–261 (2012).

    Article 

    Google Scholar 

  • 53.

    Enström, S. et al. Brucella seroprevalence in cattle near a wildlife reserve in Kenya. BMC Res. Notes 10, 2 (2017).

    Article 

    Google Scholar 

  • 54.

    Godfroid, J. Brucellosis in wildlife. Rev. Sci. Tech. 21, 277–286 (2002).

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 55.

    Martin, C., Pastoret, P. P., Brochier, B., Humblet, M. F. & Saegerman, C. A survey of the transmission of infectious diseases/infections between wild and domestic ungulates in Europe. Vet. Res. 42, 70 (2011).

    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • 56.

    Godfroid, J. et al. A ‘One Health’ surveillance and control of brucellosis in developing countries: Moving away from improvisation. Comp. Immunol. Microbiol. Infect. Dis. 36, 241–248 (2013).

    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 57.

    Kamath, P. L. et al. Genomics reveals historic and contemporary transmission dynamics of a bacterial disease among wildlife and livestock. Nat. Commun. 7, 11448 (2016).

    ADS 
    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • 58.

    Michel, A. L. et al. Wildlife tuberculosis in South African conservation areas: Implications and challenges. Vet. Microbiol. 112, 91–100 (2006).

    ADS 
    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 59.

    Pandey, G. S. et al. Serosurvey of brucella spp. infection in the Kafue Lechwe (Kobus leche kafuensis) of the Kafue flats in Zambia. Indian Vet. J. 76, 275–278 (1999).

    Google Scholar 

  • 60.

    Olsen, S. & Tatum, F. Swine brucellosis: Current perspectives. Vet. Med. Res. Rep. 8, 1–12 (2016).

    Google Scholar 

  • 61.

    Menshawy, A. M. S. et al. Assessment of Genetic Diversity of Zoonotic Brucella spp. Recovered from Livestock in Egypt Using Multiple Locus VNTR Analysis. (2014). doi:https://doi.org/10.1155/2014/353876

  • 62.

    Ibrahim, S. Studies on swine brucellosis in Egypt. J. Egypt Vet. Med. Assoc. 56, 1–12 (1996).

    Google Scholar 

  • 63.

    Ledwaba, B., Mafofo, J. & Van Heerden, H. Genome sequences of Brucella abortus and Brucella suis strains isolated from Bovine in Zimbabwe. Genome Announc. 2, 1063–1077 (2014).

    Article 

    Google Scholar 

  • 64.

    Fretin, D. et al. Unexpected Brucella suis biovar 2 infection in a dairy cow, Belgium. Emerging Infectious Diseases 19, 2053–2054 (Centers for Disease Control and Prevention, 2013).

  • 65.

    Maurin, M. Brucellosis at the dawn of the 21st century. Médecine Mal. Infect. 35, 6–16 (2005).

    CAS 
    Article 

    Google Scholar 

  • 66.

    Whatmore, A. M. et al. Brucella papionis sp. nov., isolated from baboons (Papio spp.). Int. J. Syst. Evol. Microbiol. 64, 4120–4128 (2014).

    PubMed 
    PubMed Central 
    Article 
    CAS 

    Google Scholar 

  • 67.

    Godfroid, J., Garin-Bastuji, B., Saegerman, C. & Blasco, J. M. Brucellosis in terrestrial wildlife. Rev. Sci. Tech. Off. Int. Epiz. 32, 27–42 (2013).

    CAS 
    Article 

    Google Scholar 

  • 68.

    Barendregt, J. J., Doi, S. A., Lee, Y. Y., Norman, R. E. & Vos, T. Meta-analysis of prevalence. J. Epidemiol. Community Health 67, 974–978 (2013).

    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 69.

    EpiGear. EpiGear International. Available at: http://www.epigear.com/. (Accessed: 8th February 2018)

  • 70.

    Doi, S. A. R. R., Barendregt, J. J., Khan, S., Thalib, L. & Williams, G. M. Advances in the meta-analysis of heterogeneous clinical trials I: The inverse variance heterogeneity model. Contemp. Clin. Trials 45, 130–138 (2015).

    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 71.

    Higgins, J. P. T., Thompson, S. G., Deeks, J. J. & Altman, D. G. Measuring inconsistency in meta-analyses. BMJ 327, 557–560 (2003).

    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • 72.

    Heisch, R. B., Cooke, E. R., Harvey, A. E. & De Souz, F. The isolation of Brucella suis from rodents in Kenya. East Afr. Med. J. 40, 132–133 (1963).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • 73.

    Motsi, T. R., Tichiwangana, S. C., Matope, G., Mukarati, N. L. & Studies, V. A serological survey of brucellosis in wild ungulate species from five game parks in Zimbabwe. Onderstepoort. J. Vet. Res. 80, 586 (2013).

    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 74.

    Roth, H. H. A survey of brucellosis in game animals in Rhodesia. Bull. Epizoot. Dis. Afr. Bull. des Epizoot en Afrique 15, 133–142 (1967).

    CAS 

    Google Scholar 

  • 75.

    Condy, J. B. & Vickers, D. B. Brucellosis in buffalo in Wankie National Park. Rhod. Vet. J. 8, 58–60 (1976).

    Google Scholar 

  • 76.

    Caron, A. et al. Relationship between burden of infection in ungulate populations and wildlife/livestock interfaces. Epidemiol. Infect. 141, 1522–1535 (2013).

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 77.

    Gomo, C. et al. Detection of Brucella abortus in Chiredzi district in Zimbabwe. Onderstepoort. J. Vet. Res. 79, 1–5 (2012).

    Article 

    Google Scholar 

  • 78.

    Chaparro, F., Lawrence, J. V., Bengis, R. & Myburgh, J. G. A serological survey for brucellosis in buffalo (Syncerus caffer) in the Kruger National Park. J. S. Afr. Vet. Assoc. 61, 110–111 (1990).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • 79.

    Fischer-Tenhagen, C., Hamblin, C., Quandt, S., Frö;lich, K. & Frö Lich, K. Serosurvey for selected infectious disease agents in free-ranging black and white rhinoceros in Africa. Journal of Wildlife Diseases 36, 316–323 (2000).

  • 80.

    Caron, A. et al. African buffalo movement and zoonotic disease risk across transfrontier conservation areas Southern Africa. Emerg. Infect. Dis. 22, 277–280 (2016).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • 81.

    Herr, S. & Marshall, C. Brucellosis in free-living African buffalo (Syncerus caffer): A serological survey. Onderstepoort. J. Vet. Res. 48, 133–134 (1981).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • 82.

    De Vos, V., Van Niekerk, G. A. W. J. & McConell, E. E. A survey of selected bacteriological infections of the Chacma Baboon Papio Ursinus from the Kruger National Park. Koedoe 16, 1–10 (1973).

    Google Scholar 

  • 83.

    Hamblin, C., Anderson, C. E., Jago, M., Mlengeya, T. & Hirji, K. Antibodies to some pathogenic agents in free-living wild species in Tanzania. Epidemiol. Infect. 105, 585–594 (1990).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • 84.

    Assenga, J. A., Matemba, L. E., Muller, S. K., Malakalinga, J. J. & Kazwala, R. R. Epidemiology of Brucella infection in the human, livestock and wildlife interface in the Katavi-Rukwa ecosystem, Tanzania. BMC Vet. Res. 11, 8 (2015).

    Article 

    Google Scholar 

  • 85.

    Sachs, R., Staak, C. & Groocock, C. M. Serological investigation of brucellosis in game animals in Tanzania. Bull. Epizoo. Dis. Afr. 16, 93–100 (1968).

    CAS 

    Google Scholar 

  • 86.

    Fyumagwa, R. D., Wambura, P. N., Mellau, L. S. B. & Hoare, R. Seroprevalence of Brucella abortus in buffaloes and wildebeests in the Serengeti ecosystem: A threat to humans and domestic ruminants. Tanzania Vet. J. 26, 2 (2010).

    Google Scholar 

  • 87.

    Matope, G. et al. Evaluation of sensitivity and specificity of RBT, c-ELISA and fluorescence polarisation assay for diagnosis of brucellosis in cattle using latent class analysis. Vet. Immunol. Immunopathol. 141, 58–63 (2011).

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 88.

    Muma, J. B. et al. Serosurvey of Brucella Spp Infection in the Kafue Lechwe (Kobus Leche Kafuensis) of the Kafue Flats in Zambia. J. Wildl. Dis. 46, 1063–1069 (2010).

    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 89.

    Waghela, S. Animal brucellosis in Kenya: A review. Bull. Anim. Heal. Prod. Afr. 24, 53–59 (1976).

    CAS 

    Google Scholar 

  • 90.

    Waghela, S., Karstad, L., Waghela, A. S. & Karstad, L. Antibodies to Brucella Spp among blue wildebeest and African Buffalo in Kenya. J. Wildl. Dis. 22, 189–192 (1986).

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 91.

    Magwedere, K. et al. Brucellae through the food chain: the role of sheep, goats and springbok (Antidorcus marsupialis) as sources of human infections in Namibia. J. South Afr. Vet. Assoc. Van Die Suid-Afrikaanse Veterinere Ver 82, 205–212 (2011).

    CAS 

    Google Scholar 

  • 92.

    Karesh, W. B. et al. Health evaluation of black-faced impala (Aepyceros melampus petersi) using blood chemistry and serology. J. Zoo. Wildl. Med. 28, 361–367 (1997).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • 93.

    Cooper, A. C. D. & Carmichael, I. H. The incidence of brucellosis in game in Botswana. Bull. Epizoot. Dis. Afr. 22, 119–124 (1974).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • 94.

    Thimm, B. Brucellosis in Uganda.pdf. Bull Epizoot Dis Africa 20, 43–56 (1972).

  • 95.

    Tanner, M. et al. Bovine tuberculosis and brucellosis in cattle and african buffalo in the limpopo national park mozambique. Transbound Emerg. Dis. 62, 632–638 (2015).

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 96.

    Gomo, C., de Garine-Wichatitsky, M., Caron, A. & Pfukenyi, D. M. Survey of brucellosis at the wildlife-livestock interface on the Zimbabwean side of the Great Limpopo Transfrontier Conservation Area. Trop. Anim. Health Prod. 44, 77–85 (2012).

    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • 97.

    Herr, S. & Marshall, C. Brucellosis in Free-Living African Buffalo (Syncerus-Caffer)—a Serological Survey. Onderstepoort. J. Vet. Res. 48, 133–134 (1981).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 


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