Abstract
Mangrove sediments, being organic-rich and anoxic, host diverse and functionally important microorganisms that play crucial roles in global biogeochemical cycling. In order to characterize this diversity at the genome-resolved level, we collected 38 sediment samples encompassing both surface (0–5 cm) and core (up to 90 cm) depths from six representative mangrove sites across Guangxi Province, China. Using a standardized pipeline for assembly, binning, and dereplication, we reconstructed 2,965 non-redundant metagenome-assembled genomes (MAGs), comprising 2,383 bacterial and 582 archaeal genomes spanning 78 microbial phyla. This dataset captures the high microbial diversity and functional potential within mangrove sediments under variable environmental conditions. It provides a valuable genomic resource for investigating the structure, metabolism, and ecological roles of sediment microbial communities in intertidal, nutrient-rich ecosystems, supporting future studies on microbial adaptation and biogeochemical cycling in global blue carbon environments.
Data availability
The raw sequencing dataset has been deposited in NCBI (PRJNA1270782), and the metagenome-assembled genomes (MAGs) have been deposited in the ENA (PRJEB96880) and the figshare database (https://doi.org/10.6084/m9.figshare.29320385).
Code availability
All in-house code used in this paper is available through a GitHub repository at https://github.com/SongzeCHEN/MetaGenome-MAG-Analysis.
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Acknowledgements
This work was supported by the Natural Science Foundation of Guangxi, China (Project Nos. 2024GXNSFBA010371 and 2025GXNSFHA069226), the Beibu Gulf University High-level Talent Scientific Research Start-up Project (Project No. 23KYQD18), the Natural Science Foundation of Guangxi, China (Project No. 2025GXNSFHA069232), the Improving the Basic Scientific Research Capability of Young and Middle-aged Teachers in Guangxi Colleges Project (Project No. 2025KY0471), Science and Technology Bases and Talents Special Project in Guangxi (AD22035181), Marine Science and Technology Innovation Cooperation Foundation of Beibu Gulf Project (Project No. 03190010), Development of Utilization Technology of Probiotics in Fish Gut of Symbiotic System Project (Project No. 02040772), and National College Student Innovation and Entrepreneurship Training Program (Project No. S202511607100 and S202411607011).
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Y.L., S.C. and R.B. designed the study. Y.L., S.C., H.L., Y.S., J.J. and R.B. performed the data analysis, prepared the figure and tables, wrote the paper, and revised the manuscript. Y.L. and S.C. collected the samples and conducted the experimental procedures. S.C., H.L., N.M., Y.L., J.S., D.S., M.L., J.C., J.S., B.G., J.J. and R.B. provided helpful comments and suggestions to improve the manuscript. All co-authors read and approved the final manuscript.
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Liu, Y., Chen, S., Li, H. et al. Reconstruction of 2,965 Microbial Genomes from Mangrove Sediments across Guangxi, China.
Sci Data (2025). https://doi.org/10.1038/s41597-025-06438-y
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DOI: https://doi.org/10.1038/s41597-025-06438-y
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