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UAVs indeed proved to be a practical, efficient, and accurate tool in sampling insects within four different habitats in Quebec. Furthermore, different drone settings of speed, height, and net diameter may yield different insect orders, which can be useful in studies that aim to target specific insects. Nonetheless, only height, and not speed, net diameter or drone type influenced insect abundance. Compared with Lindgren funnels, drones were not only able to catch more insects in less time, but also a wider array of the insect community diversity.Our study successfully shows the promise of using drones to collect forest and wetland canopy arthropods. More arthropods were collected flying at zero meters (grazing the canopy) than flying at one meter, while different speed, net size and drone type had less of an effect on insect yield (Fig. 2). The one-meter setting was expected to yield different arthropod diversity, such as fewer terrestrial families (ex. Araneae) and more aerial families (ex. Diptera) compared to the grazing zero-meter setting. However, the proportions of the top three orders (Diptera, Hemiptera, and Araneae) were similar among settings (Fig. 3). The capture of arachnids at one meter above the canopy can be explained by webs that are attached to taller foliage in proximity to the area, or spiders ‘ballooning’ in the airspace on silk threads25. Because canopy height was not always uniform, flying while grazing the canopy underneath the drone was at times lower than other parts of the canopy. Another explanation could be jumping spiders (ex. family Salticidae) which have been found to react to a disturbance or threat by leaping, possibly into the drone net26. Though the main three orders were in similar proportion, the one-meter setting caught five fewer orders in total than the zero-meter setting did. Flying at one meter was the only setting that captured no insects of order Coleoptera, Hymenoptera, or Orthoptera, suggesting that these orders spend time in and among the wetland canopy, and are seldom above the grassy canopy (Fig. 3). Most importantly, this setting only caught nine insects total over all flights, revealing itself to be an inefficient method of insect collection. This can be due to the number of insects available to be collected at each height. When flying at one meter, the net has access to only aerial insects in flight above the canopy (ex. flies). Flying while grazing the canopy, however, gives the researcher access to the same aerial insects in flight above the canopy, but also aerial insects in flight within the canopy (ex. bees), aerial insects at rest on the canopy (ex. leafhoppers), and terrestrial insects on the canopy (ex. ants). Thus, flying the drone while grazing the canopy opens the possibility of capturing three more insect groups compared to flying above the canopy. It is also possible that there are indeed many insects to be caught solely in the airspace, but that the ideal height for collecting insects strictly above the canopy is either less than or greater than one meter—which is the only height above the canopy that we tested.This sampling period caught three total insects from order Odonata, with two of the three being caught with the 18-inch diameter net setting (Fig. 3). As these dragonflies are typically fast flyers and of large body size, perhaps the extra diameter of the larger net was helpful in increasing the chances of catching Odonates, though we do not have enough data to make solid conclusions. This would be a valuable line of future research for studies focused on dragonflies, or other large and fast-flying insects.Flying the drone and hanging sweep net at 20 km/hr yielded the highest number and proportion of insects in the order Hemiptera, which are often found at rest within the canopy27. We speculate that the faster speed of the drone striking the grassy canopy more swiftly, thus giving the insects resting on the grasses less of an opportunity to evade the threat of the approaching net. Future studies targeting the collection of true bugs should utilize a faster drone speed in flight to optimize yield.With 84% of insects found within the second layer of our net, we conclude that our novel net design with two layers of tulle is satisfactory in retaining insects and preventing most from escaping when landing the drone. In addition to the insects counted, we never witnessed any insects flying out during landing stages. We believe that our methodology of flying the drone in quickly and covering the opening of the net with cardboard before landing the drone, in addition to the extra layer of netting, was successful at retaining the insects caught. Determining how to fly the drone and net over the two forest canopy habitats was a challenge. When flying, it was impossible for the drone camera to look both forward—to see obstacles coming up, and downwards—to see how close the net was hanging regarding the top of the canopy. For this reason, we used a second drone as a spotter for the first, the pilot of which could give instructions on moving up or down. Forest canopies were particularly difficult, as the height from one tree to the next was always different, the drone had to be constantly adjusted. We experienced many snags on branches, although they were not damaging to the net or drone. Once we became comfortable flying the drone low enough to graze the canopy, snagging became a common occurrence that was easily remedied. In fact, snagging the net probably helped in the collection of insects on those branches—a technique that could be honed and used in future studies using nets and drones over forest canopies.Over our 12 days of sampling habitat canopies with drones, we were able to determine that wetlands had the highest diversity and abundance of the four habitats examined, with lake habitats showing the lowest Shannon-Weiner Diversity index (H’), and the highest Pielou’s evenness index (J). It is unsurprising that lakes showed the most even distribution of families, as is often the case with habitats having low species richness, as there are less competitors that could dominate the habitat28. Habitat, humidity, and temperature were the most important variables affecting drone insect yield, with habitat being the common variable in all high scoring models. Wetlands had by the far the most insects collected, in addition to the highest diversity and species richness. This can be explained simply by the plant composition in wetlands compared to the other habitats. While coniferous and deciduous forests are dominated by a few species (and lakes have little to no vegetation over the water) wetlands can host a wide variety of plant species. Because insect diversity correlates with plant richness and abundance, wetlands can provide shelter and sustenance for many more groups of insects that the other habitats we studied29.Lindgren funnels disproportionately collected insects from order Coleoptera (Fig. 7). Although Lindgren funnels have been used in papers reporting results focused on insects of orders Hemiptera30,31,32,33 and Diptera34,35,36, it is unclear whether some were targeted studies or all simply bycatch of the funnel from other experiments. Instead, Lindgren funnels are overwhelmingly used in Coleoptera studies as the funnels resemble a tree and attracts various wood-boring beetles37,38,39,40,41. This attraction explains the large number and proportion of beetles caught in funnels in this study. However, diversity indices show that in three of four habitats, drones collect a higher diversity sample than the Lindgren funnels (Tables 1 and 2). Thus, though Lindgren funnels are undoubtedly effective at collecting beetles from the environment, our results indicate that the drone collection method is preferable when seeking an accurate representation of the insect diversity of the habitat. Studies focused on Coleoptera could also employ this method, which would be helpful in determining the status and proportion of beetles within the population and compared to other insect orders.In addition to the larger diversity collected by drones, the temporal advantage of this technique over the funnels can not be understated. During our study, it took three Lindgren funnel traps established for seven days to collect a total of 36 insects at the wetland sites (0.001 insect collected per minute). Comparatively, at the same height and placement, drones were able to collect 391 insects in only a combined 36 min (10.9 insects collected per minute) (Fig. 7). This large difference in both yield and time scale demonstrates that the drone collection method is vastly more efficient at arthropod sampling compared to the Lindgren funnels.While this study was successful at validating the usefulness of drones in canopy entomology studies and insect collection in general, it does have its limitations. Optimal drone settings were only examined at wetland grassy canopy sites, and it is possible that the drone might perform differently within different habitats. For example, grazing the canopy at 20 km/hr might result in high insect yield at wetlands, where the lack of obstacles made it relatively easy to fly quickly. But the same settings may be unrealistic and prone to net snagging when sampling over other habitats, such as the coniferous forest canopy. Furthermore, Lindgren funnels were an acceptable comparison to drone collection for yield and diversity at some habitats, however it was impossible to get the funnels up into the canopy where sampling took place at coniferous and deciduous sites. There is no doubt that the advantage of this method lies in its accessibility, speed, and safety—studies that need more precise and fine sampling might not benefit from drones.Overall, our research demonstrates that drones are an efficient and accurate tool in collecting a wide diversity of insects above the canopies of different habitats. Benefits included rapidly and safely sampling the airspace while drawbacks included battery life limiting the duration of sampling. If this new technique is integrated into the field of entomology, canopy studies can be done much more often, for less money, and more safely than they have been done using other techniques. In 2019, a review of the potential causes of decline of aerial insectivores concluded that insect declines and changes in high quality prey availability could be a large driver of insectivore declines9. However, there is a lack of research detailing insect trends over time. The drone collection method used in this study could provide the missing link between the need for more research of aerial canopy insects and the limitations of the current methodology in entomology. This technique can be used in conjunction with aerial insectivore surveys and diet studies to begin to determine the relationship between declining predators and prey. Future research may also use and add to our guidelines to customize drone and net settings for studies targeting specific insect orders or families. More

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