Philippa Kaur

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.This is a summary of: Needham, D. M. et al. The microbiome of a bacterivorous marine choanoflagellate contains a resource-demanding obligate bacterial associate. Nat. Microbiol. https://doi.org/10.1038/s41564-022-01174-0 (2022). More

Hydrological changes in high-latitude peatlandsWe observed three hydrological pathways, i.e., drying, wetting, and fluctuating, for both peatland clusters, non-permafrost and permafrost peatlands (Fig. 2). Approximately 54% of the studied peatlands have shifted towards drier surface conditions since 1800 CE and more intensively since 1900 CE (Fig. 2a, d), which is in line with the post-LIA warming. The overall change point to drier conditions was dated to ca. 1950 CE for non-permafrost sites and ca. 1890 CE for the permafrost compiled group. Approximately 32% of the studied peatlands have shifted towards wetter conditions (Fig. 2b, e). The overall shifting point to wetter conditions was dated to ca. 1995 CE for non-permafrost peatlands and to ca. 1990 CE for permafrost peatlands. Wetting has been especially intensive since 1900 CE for non-permafrost peatlands and since 1950 CE for permafrost peatlands. Interestingly, the data showed that in permafrost peatlands a significant dry shift always preceded a wet shift (Fig. 2e). Approximately 14% of the studied peatlands indicated no clear trend, with fluctuating hydrological conditions (Fig. 2c, f).Non-permafrost peatlands generally showed spatially extensive drying across the northern high latitudes, apart from northeastern Canada, where a wetting trend was more frequently observed. Permafrost peatlands, however, were more variable, with some drying, some wetting, and no overall coherent regional pattern was visible (Fig.1a, b). It should be noted that peatlands synthesized here have undergone little or no direct human impact, i.e., their surface hydrology was not significantly affected by human disturbances such as drainage, when compared to, for example, central European peatlands discussed in Swindles et al. (2019)5. This implies that climate and/or local topographical forcing are the predominant hydrological drivers in this study. The dataset is to some extent biased as there are more non-permafrost records from northeastern Canada but more permafrost records from northern Sweden and this might result in regional overestimation to either wetting or drying trends. Nevertheless, the pattern of diverse timing of the hydrological shifts between the individual coring points (Fig. 2) indicates the variability in sensitivity of different regions/peatlands to climate changes.Potential links to climate change and permafrost dynamicsThe comparison of the reconstructed water table and climate data suggests that climate, especially summer temperature, has played an important role in shaping the peatland water table (Fig. 1c–f). The pattern detected here for non-permafrost peatlands, an extensive drying, is comparable to that observed for central European peatlands5. In addition to direct climate forcing, a recent acceleration of peat accumulation might partly explain the drying trend by disconnecting the peatland surface from the water table17. However, for northeastern Canada a wetting trend has been observed more often, possibly regulated by the regional climate that shows clearly less warming in the focused period compared to other regions (Fig. 1c, d).Permafrost initiation in the past caused a peat surface uplift and is probably detected as a dry phase (Fig. 2d, e). Post-LIA warming-induced increase in evapotranspiration may have strengthened the surface drying which originally resulted from surface-uplift and probably mitigated the gradual wetting related to permafrost thawing11. The level of warming has varied among the regions. In some areas such as northeastern Canada temperature has increased less and, when combined with higher precipitation or higher effective moisture level, may have caused surface wetting in permafrost peatlands. This is a direct climate forcing rather than permafrost thawing, which is a consequence of climate warming, i.e., more indirect climate forcing. To date, it is yet challenging to estimate any one tipping point of warming that might trigger permafrost thawing, as the local conditions vary from bottom ground soil conditions to hydrology and vegetation. The consequent wetting or drying depends on evapotranspiration and ice richness etc., which further challenges the prediction of hydrological conditions of permafrost peatlands.The divergent three moisture patterns may occur in the same region and even in the same peatland, especially if the permafrost is present. This complex response pattern is well supported by the records from the Abisko region, Sweden, where replicated sampling was carried out, and captured different successional stages of local permafrost peatlands7,18. In contrast to permafrost peatlands, non-permafrost peatlands are more likely to experience a more consistent ecosystem response pattern19 as supported by the replicated records suggesting the same pattern happening simultaneously in several regions (Fig. 1a). The fluctuating pattern of many records reported here suggests that the past and recent climate has not yet caused a state change in hydrological conditions.Insights into carbon dynamics and future perspectiveGenerally, our results suggest that the recent climate warming has caused hydrological shifts in most high-latitude peatlands, highlighting its pronounced effect on shaping peatland moisture balance, and further on driving peatland C balance. It has been reported that a 1-cm water-table drawdown would increase 3.3–5.0 mg CO2 m−2 h−1 and decrease 2.2–3.6 mg CO2-eq m−2 h−1 (CH4) to the atmosphere, and the average sensitivity of CO2 and CH4 combined was 0.8–2.3 mg CO2-eq m−2 h−1 cm−1 according to a global scale analysis, including sites from high-latitudes3. However, it should be noted that the sensitivity of greenhouse gas fluxes to the magnitude of hydrological changes might vary among different regions and peatland types. It appears that most pan-Arctic peatlands are undergoing a drying trend, that may lead to a decreased C sink capacity3,19, if not compensated by increased C uptake from the atmosphere20.It is very likely that over the 21st century warming in high latitudes will continue to be more pronounced than the global average21. Precipitation is projected to increase, albeit with large regional variability. Also, extreme events with heavy rainfall and drought are becoming more frequent and intense22. It is estimated that about 20% of permafrost zone is experiencing accelerated and abrupt permafrost thaw that is likely causing wetting conditions4, while gradual permafrost thaw has been observed across the circumpolar regions23. Both an increase in precipitation and permafrost thaw might mitigate the drying pressure caused by warming and increased evapotranspiration. However, abrupt permafrost thaw in peatlands can result in a rapid (over years to decades) loss of C from the formerly frozen permafrost peat, causing these peatlands to be a net source of C to the atmosphere before post-thaw accumulation returns them to a net sink (centuries to millennia)12,13. The future C sink and source function of peatlands is a key element in contributing to climate change, but the observed divergent pathways of peatland hydrological successions further challenge the projections of high-latitude peatland C sink and source dynamics. Conversely, it clearly highlights the importance of climate forcing in peatland succession scenarios. Our study reveals that the response of high-latitude peatlands to changing climate conditions is complex. We detect variable ecohydrological trajectories, and in the future, these will determine the C sink capacity of northern peatlands. The observed patterns inevitably create challenges for the climate change modelling community. How to capture the highly heterogenic successional pathways of northern peatlands needs to be a key research focus. More

Erwin, D. H. & Tweedt, S. Ecological drivers of the Ediacaran-Cambrian diversification of Metazoa. Evol. Ecol. 26, 417–433 (2012).Article 

Google Scholar 
Laflamme, M., Darroch, S. A., Tweedt, S. M., Peterson, K. J. & Erwin, D. H. The end of the Ediacara biota: Extinction, biotic replacement, or Cheshire Cat?. Gondwana Res. 23, 558–573 (2013).ADS 
Article 

Google Scholar 
Mángano, M. G. & Buatois, L. A. Decoupling of body-plan diversification and ecological structuring during the Ediacaran-Cambrian transition: Evolutionary and geobiological feedbacks. Proc. R. Soc. B-Biol. Sci. 281, 20140038 (2014).Article 

Google Scholar 
Mángano, M. G. & Buatois, L. A. The Cambrian revolutions: Trace-fossil record, timing, links and geobiological impact. Earth-Sci. Rev. 173, 96–108 (2017).ADS 
Article 

Google Scholar 
Mángano, M. G. & Buatois, L. A. The rise and early evolution of animals: Where do we stand from a trace-fossil perspective?. Interface Focus 10, 20190103 (2020).PubMed 
PubMed Central 
Article 

Google Scholar 
Darroch, S. A. et al. Biotic replacement and mass extinction of the Ediacara biota. Proc. R. Soc. B 282, 20151003 (2015).PubMed 
PubMed Central 
Article 

Google Scholar 
Darroch, S. A., Smith, E. F., Laflamme, M. & Erwin, D. H. Ediacaran extinction and Cambrian explosion. Trends Ecol. Evol. 33, 653–663 (2018).PubMed 
Article 

Google Scholar 
Schiffbauer, J. D. et al. The latest Ediacaran Wormworld fauna: Setting the ecological stage for the Cambrian explosion. GSA Today 26, 4–11 (2016).Article 

Google Scholar 
Zamora, S., Deline, B., Javier Álvaro, J. & Rahman, I. A. The Cambrian Substrate Revolution and the early evolution of attachment in suspension-feeding echinoderms. Earth-Sci. Rev. 171, 478–491 (2017).ADS 
Article 

Google Scholar 
Hantsoo, K. G., Kaufman, A. J., Cui, H., Plummer, R. E. & Narbonne, G. M. Effects of bioturbation on carbon and sulfur cycling across the Ediacaran-Cambrian transition at the GSSP in Newfoundland, Canada. Can. J. Earth Sci. 55, 1240–1252 (2018).ADS 
CAS 
Article 

Google Scholar 
Boyle, R. A., Dahl, T. W., Bjerrum, C. J. & Canfield, D. E. Bioturbation and directionality in Earth’s carbon isotope record across the Neoproterozoic-Cambrian transition. Geobiology 16, 252–278 (2018).CAS 
PubMed 
Article 

Google Scholar 
van de Velde, S., Mills, B. J., Meysman, F. J., Lenton, T. M. & Poulton, S. W. Early Palaeozoic ocean anoxia and global warming driven by the evolution of shallow burrowing. Nat. Commun. 9, 2554 (2018).ADS 
PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
Gougeon, R. C., Mangano, M. G., Buatois, L. A., Narbonne, G. M. & Laing, B. A. Early Cambrian origin of the shelf sediment mixed layer. Nat. Commun. 9, 1909 (2018).ADS 
PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
Seilacher, A. & Pflüger, F. In Biostabilization of Sediments (eds. Krumbein W. E., Peterson D. M., & Stal L. J.) 97–105 (Bibliotheks und Informationsystem der Carl von Ossietzky Universität, 1994).Seilacher, A. Biomat-related lifestyles in the Precambrian. Palaios 14, 86–93 (1999).ADS 
Article 

Google Scholar 
Buatois, L. A. et al. Quantifying ecospace utilization and ecosystem engineering during the early Phanerozoic: The role of bioturbation and bioerosion. Sci. Adv. 6, eabb0618 (2020).ADS 
CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Hagadorn, J. W. & Bottjer, D. J. Restriction of a late Neoproterozoic biotope; suspect-microbial structures and trace fossils at the Vendian-Cambrian transition. Palaios 14, 73–85 (1999).ADS 
Article 

Google Scholar 
Buatois, L. A. & Mangano, M. G. Early colonization of the deep sea: Ichnologic evidence of deep-marine benthic ecology from the Early Cambrian of northwest Argentina. Palaios 18, 572–581 (2003).ADS 
Article 

Google Scholar 
Clausen, S., Álvaro, J. J. & Zamora, S. Replacement of benthic communities in two Neoproterozoic-Cambrian subtropical-to-temperate rift basins, High Atlas and Anti-Atlas, Morocco. J. Afr. Earth Sci. 98, 72–93 (2014).ADS 
Article 

Google Scholar 
Mángano, M. G. & Buatois, L. A. In The Trace-Fossil Record of Major Evolutionary Events: Volume 1: Precambrian and Paleozoic (eds. Mángano M. G. & Buatois L. A.) 73–126 (Springer Netherlands, 2016).Bayet-Goll, A., Buatois, L. A., Mangano, M. G. & Daraei, M. The interplay of environmental constraints and bioturbation on matground development along the marine depositional profile during the Ordovician Radiation. Geobiology 20, 33–270 (2022).Article 
CAS 

Google Scholar 
Bayet-Goll, A., Daraei, M., Geyer, G., Bahrami, N. & Bagheri, F. Environmental constraints on the distribution of matground and mixground ecosystems across the Cambrian Series 2–Miaolingian boundary interval in Iran: A case study for the central sector of northern Gondwana. J. Afr. Earth Sci. 176, 104120 (2021).Article 

Google Scholar 
Minter, N. J. et al. In The Trace-Fossil Record of Major Evolutionary Events: Volume 1: Precambrian and Paleozoic (eds. Mángano M. G. & Buatois L. A.) 157–204 (Springer Netherlands, 2016).Minter, N. J. et al. Early bursts of diversification defined the faunal colonization of land. Nat. Ecol. Evol. 1, 0175 (2017).Article 

Google Scholar 
Nemec, W. & Steel, R. J. In Fan Deltas: sedimentology and tectonic settings (eds. Nemec W. & Steel R. J.) 3–13 (Blackie and Son, 1988).Postma, G. An analysis of the variation in delta architecture. Terra Nova 2, 124–130 (1990).ADS 
Article 

Google Scholar 
Prior, D. B. & Bornhold, B. D. Submarine sedimentation on a developing Holocene fan delta. Sedimentology 36, 1053–1076 (1989).ADS 
Article 

Google Scholar 
Piper, D. J. W., Kontopoulos, N., Anagnostou, C., Chronis, G. & Panagos, A. G. Modern fan deltas in the western Gulf of Corinth, Greece. Geo-Mar. Lett. 10, 5–12 (1990).ADS 
Article 

Google Scholar 
Rasmussen, H. Nearshore and alluvial facies in the Sant Llorenç del Munt depositional system: Recognition and development. Sediment. Geol. 138, 71–98 (2000).ADS 
Article 

Google Scholar 
Steel, R., Rasmussen, H., Eide, S., Neuman, B. & Siggerud, E. Anatomy of high-sediment supply, transgressive tracts in the Vilomara composite sequence, Sant LlorencË del Munt, Ebro Basin, NE Spain. Sediment. Geol. 138, 125–142 (2000).ADS 
Article 

Google Scholar 
Zavala, C. et al. Deltas: A new classification expanding Bates’s concepts. J. Palaeogeogr. 10, 23 (2021).ADS 
Article 

Google Scholar 
Ekdale, A. A. & Lewis, D. W. Trace fossils and paleoenvironmental control of ichnofacies in a late Quaternary gravel and loess fan delta complex, New Zealand. Palaeogeogr. Palaeoclimatol. Palaeoecol. 81, 253–279 (1991).Article 

Google Scholar 
Buatois, L. A. & Mángano, M. G. Ichnology: Organism-substrate Interactions in Space and Time (Cambridge University Press, 2011).Book 

Google Scholar 
Hovikoski, J., Uchman, A., Alsen, P. & Ineson, J. Ichnological and sedimentological characteristics of submarine fan-delta deposits in a half-graben, Lower Cretaceous Palnatokes Bjerg Formation, NE Greenland. Ichnos 26, 28–57 (2019).Article 

Google Scholar 
Sendra, J., Reolid, M. & Reolid, J. Palaeoenvironmental interpretation of the Pliocene fan-delta system of the Vera Basin (SE Spain): Fossil assemblages, ichnology and taphonomy. Palaeoworld 29, 769–788 (2020).Article 

Google Scholar 
Kreis, L. K. et al. Lower Paleozoic map series: Saskatchewan. Miscellaneous Report 2004–8 (CD-ROM) (Saskatchewan Industry and Resources, 2004).
Google Scholar 
Marsh, A. & Love, M. In Saskatchewan Ministry of the Economy, Saskatchewan Geological Survey, Saskatchewan Geological Survey Vol. Open File 2014-1, set of 156 maps (2014).Sawatzky, H. B., Agarwal, R. G. & Wilson, W. Helium prospects of southwest Saskatchewan. 26 (Saskatchewan Department of Mineral Resources, 1960).Fyson, W. K. Deadwood and Winnipeg stratigraphy in southwestern Saskatchewan. Report 64, 37 (Saskatchewan Department of Mineral Resources, 1961).Kent, D. M. Paleotectonic controls on sedimentation in northern Williston Basin area, Saskatchewan. AAPG Bull. 67, 1345–1345 (1983).
Google Scholar 
Kent, D. M. & Haidl, F. M. The distribution of Ashern and Winnipegosis strata (Middle Devonian) on the Swift Current Platform, southern Saskatchewan. Summary of Investigations, Miscellaneous Report 93-4, 201–206 (Saskatchewan Geological Survey, Saskatchewan Energy and Mines, 1993).MacEachern, J. A., Zaitlin, B. A. & Pemberton, S. G. A sharp-based sandstone of the Viking Formation, Joffre Field, Alberta, Canada; criteria for recognition of transgressively incised shoreface complexes. J. Sediment. Res. 69, 876–892 (1999).ADS 
Article 

Google Scholar 
Pemberton, S. G., Frey, R. W. & Bromley, R. G. The ichnotaxonomy of Conostichus and other plug-shaped ichnofossils. Can. J. Earth Sci. 25, 866–892 (1988).ADS 
Article 

Google Scholar 
Hall, J. & Whitfield, R. P. Paleontology. In US Geol. Expl. 40th Par. Rept. 4, 197–302 (1877).Walcott, C. D. Cambrian and Lower Ozarkian trilobites. Smithson. Misc. Coll. 75, 53–60 (1924).
Google Scholar 
Meek, F. B. Preliminary paleontological report, consisting of lists and descriptions of fossils, with remarks on the ages of the rocks in which they were found. In U. S. Geol. Surv. Terr. 6th Ann. Rept., 429–518 (1873).Walcott, C. D. Cambrian geology and paleontology of Cambrian trilobites. Smithson. Misc. Coll. 64, 157–258 (1916).
Google Scholar 
Harding, S. C. & Ekdale, A. A. Trace fossils and glauconitic pellets provide insight into Cambrian siliciclastic marine environments. Palaios 33, 256–265 (2018).ADS 
Article 

Google Scholar 
Shillito, A. P. & Davies, N. S. The Tumblagooda Sandstone revisited: Exceptionally abundant trace fossils and geological outcrop provide a window onto Palaeozoic littoral habitats before invertebrate terrestrialization. Geol. Mag. 157, 1939–1970 (2020).ADS 
Article 

Google Scholar 
Shillito, A. P. & Davies, N. S. Archetypally Siluro-Devonian ichnofauna in the Cowie Formation, Scotland: Implications for the myriapod fossil record and Highland Boundary Fault Movement. Proc. Geol. Assoc. 128, 815–828 (2017).Article 

Google Scholar 
Buatois, L. A. et al. The invasion of the land in deep time: integrating Paleozoic records of paleobiology, ichnology, sedimentology, and geomorphology. Integr. Comp. Biol. 0, 1–35. https://doi.org/10.1093/icb/icac059 (2022).Davies, N. S. & Gibling, M. R. Paleozoic vegetation and the Siluro-Devonian rise of fluvial lateral accretion sets. Geology 38, 51–54 (2010).ADS 
Article 

Google Scholar 
McMahon, W. J. & Davies, N. S. Evolution of alluvial mudrock forced by early land plants. Science 359, 1022–1024 (2018).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Fedo, C. M. & Cooper, J. D. Braided fluvial to marine transition; the basal Lower Cambrian Wood Canyon Formation, southern Marble Mountains, Mojave Desert, California. J. Sediment. Res. 60, 220–234 (1990).
Google Scholar 
Eyre, B. Early Cambrian alluvial fan-deltas in the Georgina Basin, Australia. Aust. J. Earth Sci. 41, 27–36 (1994).ADS 
Article 

Google Scholar 
MacNaughton, R. B., Dalrymple, R. & Narbonne, G. M. Early Cambrian braid-delta deposits, MacKenzie Mountains, north-western Canada. Sedimentology 44, 587–609 (1997).ADS 
Article 

Google Scholar 
Muhlbauer, J. G. & Fedo, C. M. Architecture of a river-dominated, wave- and tide-influenced, pre-vegetation braid delta: Cambrian middle member of the Wood Canyon Formation, southern Marble Mountains, California, U.S.A. J. Sediment. Res. 90, 1011–1036 (2020).ADS 
Article 

Google Scholar 
McMahon, W. J., Davies, N. S. & Went, D. J. Negligible microbial matground influence on pre-vegetation river functioning: Evidence from the Ediacaran-Lower Cambrian Series Rouge, France. Precambrian Res. 292, 13–34 (2017).ADS 
CAS 
Article 

Google Scholar 
Mikuláš, R. Trace fossils from the Paseky Shale (Early Cambrian, Czech Republic). J. Czech Geol. Soc. 40, 37–44 (1995).
Google Scholar 
MacNaughton, R. B. & Narbonne, G. M. Evolution and ecology of Neoproterozoic-Lower Cambrian trace fossils, NW Canada. Palaios 14, 97–115 (1999).ADS 
Article 

Google Scholar 
Buatois, L. A. et al. Colonization of brackish-water systems through time: Evidence from the trace-fossil record. Palaios 20, 321–347 (2005).ADS 
Article 

Google Scholar 
Hofmann, R., Mángano, M. G., Elicki, O. & Shinaq, R. Paleoecologic and biostratigraphic significance of trace fossils from shallow- to marginal-marine environments from the Middle Cambrian (Stage 5) of Jordan. J. Paleontol. 86, 931–955 (2012).Article 

Google Scholar 
Mángano, M. G., Buatois, L. A., Hofmann, R., Elicki, O. & Shinaq, R. Exploring the aftermath of the Cambrian explosion: The evolutionary significance of marginal- to shallow-marine ichnofaunas of Jordan. Palaeogeogr. Palaeoclimatol. Palaeoecol. 374, 1–15 (2013).Article 

Google Scholar 
Mángano, M. G. et al. Were all trilobites fully marine? Trilobite expansion into brackish water during the early Palaeozoic. Proc. R. Soc. Lond. B Biol. Sci. 288, 20202263 (2021).
Google Scholar 
Siggerud, E. I. H. & Steel, R. J. Architecture and trace-fossil characteristics of a 10000–20000 Year, fluvial-to-marine sequence, SE Ebro Basin, Spain. J. Sediment. Res. 69, 365–383 (1999).ADS 
Article 

Google Scholar 
Lockley, M. G., Rindsberg, A. K. & Zeiler, R. M. The paleoenvironmental significance of the nearshore Curvolithus ichnofacies. Palaios 2, 255–262 (1987).ADS 
Article 

Google Scholar 
Folk, R. L. Petrology of Sedimentary Rocks (Hemphill Publishing Company, 1980).
Google Scholar 
Wentworth, C. K. A scale grade and class terms for clastic sediments. J. Geol. 30, 377–392 (1922).ADS 
Article 

Google Scholar 
Pettijohn, F. J., Potter, P. E. & Siever, R. Sand and Sandstone 2nd edn. (Springer, 1987).Book 

Google Scholar 
Ingram, R. L. Terminology for the thickness of stratification and parting units in sedimentary rocks. Geol. Soc. Am. Bull. 65, 937–938 (1954).ADS 
Article 

Google Scholar 
Reineck, H.-E. Sedimentgefüge im Bereich der südliche Nordsee. Abh. Senckb. Naturforsch. Ges. 505, 1–138 (1963).
Google Scholar  More

MacFarlane, G. R., Koller, C. E. & Blomberg, S. P. Accumulation and partitioning of heavy metals in mangroves: A synthesis of field-based studies. Chemosphere 69, 1454–1464 (2007).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Krauss, K. W. & Osland, M. J. Tropical cyclones and the organization of mangrove forests: A review. Ann. Bot. 125, 213–234 (2020).PubMed 

Google Scholar 
Kirk, G. J. D. & Krinzucker, H. J. The potential for nitrification and nitrate uptake in the rhizosphere of wetland plants: A modeling study. Ann. Bot. 96, 639–646 (2005).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Wu, C., Huang, L., Xue, S. G. & Pan, W. S. Oxic and anoxic conditions affect arsenic (As) accumulation and arsenite transporter expression in rice. Chemosphere 168, 969–975 (2017).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Tripathi, R. D. et al. Roles for root iron plaque in sequestration and uptake of heavy metals and metalloids in aquatic and wetland plants. Metallomics 6, 1789–1800 (2014).CAS 
PubMed 
Article 

Google Scholar 
Xiao, W. et al. Continuous flooding stimulates root iron plaque formation and reduces chromium accumulation in rice (Oryza sativa L.). Sci. Total Environ. 788, 147786 (2021).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Lee, C. H., Hsieh, Y. C., Lin, T. H. & Lee, D. Y. Iron plaque formation and its effect on arsenic uptake by different genotypes of paddy rice. Plant Soil 363, 231–241 (2013).CAS 
Article 

Google Scholar 
Dai, M. Y. et al. Phosphorus effects on radial oxygen loss, root porosity and iron plaque in two mangrove seedlings under cadmium stress. Mar. Pollut. Bull. 119, 262–269 (2017).CAS 
PubMed 
Article 

Google Scholar 
Liu, C. Y., Chen, C. L., Gong, X. F., Zhou, W. B. & Yang, J. Y. Progress in research of iron plaque on root surface of wetland plants. Acta Ecol. Sin. 34, 2470–2480 (2014).CAS 

Google Scholar 
Li, J., Liu, J. C., Yan, C. L., Du, D. L. & Li, H. L. The alleviation effect of iron on cadmium phytotoxicity on mangrove A. marina. Alleviation effect of rion on cadmium phytotoxicity in mangrove Avicennia marina (Forsk.) Vierh. Chemosphere 226, 413–420 (2019).ADS 
CAS 
Article 

Google Scholar 
Zhang, J. Y. et al. Effects of nano-Fe3O4-modified biochar on iron plaque formation and Cd accumulation in rice (Oryza sativa L.). Environ. Pollut. 260, 113970 (2020).CAS 
PubMed 
Article 

Google Scholar 
Farhat, Y. A., Kim, S. H., Seyfferth, A. L., Zhang, L. & Neumann, R. B. Altered arsenic availability, uptake, and allocation in rice under elevated temperature. Sci. Total Environ. 763, 143049 (2021).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Wu, X. Y. et al. A review of toxicity and mechanisms of individual and mixtures of heavy metals in the environment. Environ. Sci. Pollut. R. 23, 8244–8259 (2016).CAS 
Article 

Google Scholar 
Abubakar, U. S., Zulkifli, S. Z. & Ismail, A. Heavy metals bioavailability and pollution indices evaluation in the mangrove surface sediment of Sungai Puloh Malaysia. Environ. Earth. Sci. 77, 225 (2018).CAS 
Article 

Google Scholar 
Kulkarni, R., Deobagkar, D. & Zinjarde, S. Metals in mangrove ecosystems and associated biota: A global perspective. Ecotox. Environ. Safe. 153, 215–228 (2018).CAS 
Article 

Google Scholar 
Shi, C., Ding, H., Zan, Q. J. & Li, R. L. Spatial variation and ecological risk assessment of heavy metals in mangrove sediments across China. Mar. Pollut. Bull. 143, 115–124 (2019).CAS 
PubMed 
Article 

Google Scholar 
Cheng, H. et al. Mixture of Pb, Zn and Cu on root permeability and radial oxygen loss in the mangrove Bruguiera gymnorrhiza. Ecotoxicology 29, 691–697 (2020).CAS 
PubMed 
Article 

Google Scholar 
Cheng, S. S. et al. Temporal variations in physiological responses of kandelia obovata seedlings exposed to multiple heavy metals. Mar. Pollut. Bull. 124, 1089–1095 (2017).CAS 
PubMed 
Article 

Google Scholar 
Shen, X. X. et al. Does combined heavy metal stress enhance iron plaque formation and heavy metal bioaccumulation in Kandelia obovata?. Environ. Exp. Bot. 186, 104463 (2021).CAS 
Article 

Google Scholar 
Shen, X. X. et al. Interactive effects of single, binary and trinary trace metals (lead, zinc and copper) on the physiological responses of Kandelia obovata seedlings. Environ. Geochem. Hlth. 41, 135–148 (2019).CAS 
Article 

Google Scholar 
Youssef, T. & Saenger, P. Anatomical adaptive strategies to flooding and rhizosphere oxidation in mangrove seedlings. Aust. J. Bot. 44, 297–313 (1996).Article 

Google Scholar 
Cheng, H., Wang, Y. S., Fei, J., Jiang, Z. Y. & Ye, Z. H. Differences in root aeration, iron plaque formation and waterlogging tolerance in six mangroves along a continues tidal gradient. Ecotoxicology 24, 1659–1667 (2015).CAS 
PubMed 
Article 

Google Scholar 
Takahashi, H., Yamauchi, T., Colmer, T. D. & Nakazono, M. Aerenchyma formation in plants. In Low-Oxygen Stress in Plants (eds van Dongen, J. T. & Licausi, F.) 247–265 (Springer, 2014).Chapter 

Google Scholar 
Yamauchi, T., Colmer, T. D., Pedersen, O. & Nakazono, M. Regulation of root traits for internal aeration and tolerance to soil waterlogging-flooding stress. Plant Physiol. 176, 1118–1130 (2018).CAS 
PubMed 
Article 

Google Scholar 
Cheng, H. et al. The role of radial oxygen loss and root anatomy on zinc uptake and tolerance in mangrove seedlings. Environ. Pollut. 158, 1189–1196 (2010).CAS 
PubMed 
Article 

Google Scholar 
Liu, Y. et al. Mixed heavy metals tolerance and radial oxygen loss in mangrove seedlings. Mar. Pollut. Bull. 58, 1843–1849 (2009).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Wu, C., Li, H., Ye, Z., Wu, F. & Wong, M. H. Effects of As levels on radial oxygen loss and as speciation in rice. Environ. Sci. Pollut R. 20, 8334–8341 (2013).CAS 
Article 

Google Scholar 
Mendellshn, A., Kleiss, B. A. & Wakeley, J. S. Factors controlling the formation of oxidized root channels—a review. Wetlands 15, 37–46 (1995).Article 

Google Scholar 
Moller, C. L. & Sand-Jesen, K. Iron plaques improve the oxygen supply to root meristems of the freshwater plant Lobelia dortmanna. New Phytol. 179, 848–856 (2008).PubMed 
Article 
CAS 

Google Scholar 
Yang, J. X., Liu, Y. & Ye, Z. H. Root-induced changes of pH, eh, Fe (II) and fractions of Pb and Zn in rhizosphere soils of four wetland plants with different radial oxygen losses. Pedosphere 22, 518–527 (2012).CAS 
Article 

Google Scholar 
Hu, M., Li, F., Liu, C. & Wu, W. The diversity and abundance of as (III) oxidizers on root iron plaque is critical for arsenic bioavailability to rice. Sci. Rep. 5, 13611 (2015).ADS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Huang, G. X., Ding, C. F., Li, Y. S., Zhang, T. L. & Wang, X. X. Selenium enhances iron plaque formation by elevating the radial oxygen loss of roots to reduce cadmium accumulation in rice (Oryza sativa L.). J. Hazard. Mater. 398, 122860 (2020).CAS 
PubMed 
Article 

Google Scholar 
Thakur, S. et al. Plant-driven removal of heavy metals from soil: Uptake, translocation, tolerance mechanism, challenges, and future perspectives. Environ. Monit. Assess. 188, 206–212 (2016).PubMed 
Article 

Google Scholar 
Huang, H., Zhu, Y., Chen, Z., Yin, X. & Sun, G. Arsenic mobilization and speciation during iron plaque decomposition in a paddy soil. J. Soil. Sediment. 12, 402–410 (2012).CAS 
Article 

Google Scholar 
Zhong, S. Q. Effect of iron plaque on root growth and activity of two wetland plants. J. Hydroecol. 36, 74–79 (2015).
Google Scholar 
Khan, N. et al. Root iron plaque on wetland plants as a dynamic pool of nutrients and contaminants. Adv. Agron. 138, 1–96 (2016).Article 

Google Scholar 
Ma, H. H. et al. Formation of iron plaque on roots of Iris pseudacorus and its consequence for cadmium immobilization is impacted by zinc concentration. Ecotox. Environ. Safe. 193, 110306 (2020).CAS 
Article 

Google Scholar 
Martinez, S., Sáenz, M. E., Alberdi, J. L. & Di Marzio, W. D. Comparative ecotoxicity of single and binary mixtures exposures of cadmium and zinc on growth and biomarkers of Lemna gibba. Ecotoxicology 29, 571–583 (2020).CAS 
PubMed 
Article 

Google Scholar 
Huang, Y. Z., Hu, Y. & Liu, Y. X. Heavy metal accumulation in iron plaque and growth of rice plants upon exposure to single and combined contamination by copper, cadmium and lead. Acta Ecol. Sin. 29, 320–326 (2009).Article 

Google Scholar 
Deraison, H., Badenhausser, I., Börger, L. & Gross, N. Herbivore effect traits and their impact on plant community biomass: An experimental test using grasshoppers. Funct. Ecol. 29, 650–661 (2015).Article 

Google Scholar 
Wang, F., Wang, X. & Song, N. Polythylene microplastics increase cadmium uptake in lettuce (Lactuca sativa L.) by altering the soil microenvironment. Sci. Total Environ. 784, 147133 (2021).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Yu, H. et al. Microplastic residues in wetland ecosystems: Do they truly threaten the plant-microbe-soil system?. Environ. Int. 156, 106708 (2021).CAS 
PubMed 
Article 

Google Scholar 
He, B., Li, R. L., Chai, M. W. & Qiu, G. Y. Threat of heavy metal contamination in eight mangrove plants from the Futian mangrove forest, China. Environ. Geochem. Hlth. 36, 467–476 (2014).CAS 
Article 

Google Scholar 
Du, J. N., Yan, C. L. & Li, Z. D. Formation of iron plaque on mangrove Kandalar. Obovata (S.L.) Root surfaces and its role in cadmium uptake and translocation. Mar. Pollut. Bull. 74, 105–109 (2013).CAS 
PubMed 
Article 

Google Scholar 
Hao, Z. B., Cang, J. & Xu, Z. Plant Physiology Experiment (Harbin Institute of Technology Press, 2004).
Google Scholar 
Kludze, H. K., Delaune, R. D. & Patrick, W. H. A colorimetric method for assaying dissolved oxygen loss from container-grown rice roots. Agron. J. 86, 483–487 (1994).CAS 
Article 

Google Scholar 
Kludze, H. K., Delaune, R. D. & Patrick, W. H. Aerenchyma formation and methane and oxygen-exchange in rice. Soil Sci. Soc. Am. J. 57, 386–391 (1993).ADS 
CAS 
Article 

Google Scholar 
Mei, X. Q., Yang, Y., Tam, N. F. Y., Wang, Y. W. & Li, L. Roles of root porosity, radial oxygen loss, Fe plaque formation on nutrient removal and tolerance of wetland plants to domestic wastewater. Water Res. 50, 147–159 (2014).CAS 
PubMed 
Article 

Google Scholar 
Taylor, G. J. & Crowder, A. Use of the DCB technique for extraction of hydrous iron oxides from roots of wetland plants. Am. J. Bot. 70, 1254–1257 (1983).CAS 
Article 

Google Scholar 
USEPA (United States Environmental Protection Agency). Method 3052: microwave assisted acid digestion of siliceous and organically based matrices SW-846. DC: Washington (1996). More

Zabalza, A., Orcaray, L., Fernandez-Escalada, M., Zulet-Gonzalez, A. & Royuela, M. The pattern of shikimate pathway and phenylpropanoids after inhibition by glyphosate or quinate feeding in pea roots. Pestic Biochem. Physiol. 141, 96–102. https://doi.org/10.1016/j.pestbp.2016.12.005 (2017).CAS 
Article 
PubMed 

Google Scholar 
Amrhein, N., Deus, B., Gehrke, P. & Steinrucken, H. C. The site of the inhibition of the shikimate pathway by glyphosate: II. Interference of glyphosate with chorismate formation in vivo and in vitro. Plant Physiol. 66, 830–834. https://doi.org/10.1104/pp.66.5.830 (1980).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Landrigan, P. J. & Belpoggi, F. The need for independent research on the health effects of glyphosate-based herbicides. Environ. Health 17, 51. https://doi.org/10.1186/s12940-018-0392-z (2018).Article 
PubMed 
PubMed Central 

Google Scholar 
Tu, M. & Randall, J. Adjuvants. Tu, M. et al. Weed Control Methods Handbook the Nature Conservancy. 1–24. (TNC, 2003).Brausch, J. M. & Smith, P. N. Toxicity of three polyethoxylated tallowamine surfactant formulations to laboratory and field collected fairy shrimp, Thamnocephalus platyurus. Arch. Environ. Contam. Toxicol. 52, 217–221. https://doi.org/10.1007/s00244-006-0151-y (2007).CAS 
Article 
PubMed 

Google Scholar 
Brausch, J. M., Beall, B. & Smith, P. N. Acute and sub-lethal toxicity of three POEA surfactant formulations to Daphnia magna. Bull. Environ. Contam. Toxicol. 78, 510–514. https://doi.org/10.1007/s00128-007-9091-0 (2007).CAS 
Article 
PubMed 

Google Scholar 
Tsui, M. T. & Chu, L. M. Aquatic toxicity of glyphosate-based formulations: Comparison between different organisms and the effects of environmental factors. Chemosphere 52, 1189–1197. https://doi.org/10.1016/S0045-6535(03)00306-0 (2003).ADS 
CAS 
Article 
PubMed 

Google Scholar 
Adam, A., Marzuki, A., Abdul Rahman, H. & Abdul Aziz, M. The oral and intratracheal toxicities of ROUNDUP and its components to rats. Vet. Hum. Toxicol. 39, 147–151 (1997).CAS 
PubMed 

Google Scholar 
Howe, C. M. et al. Toxicity of glyphosate-based pesticides to four North American frog species. Environ. Toxicol. Chem. 23, 1928–1938. https://doi.org/10.1897/03-71 (2004).CAS 
Article 
PubMed 

Google Scholar 
Mesnage, R., Benbrook, C. & Antoniou, M. N. Insight into the confusion over surfactant co-formulants in glyphosate-based herbicides. Food Chem. Toxicol. 128, 137–145. https://doi.org/10.1016/j.fct.2019.03.053 (2019).CAS 
Article 
PubMed 

Google Scholar 
Mesnage, R., Bernay, B. & Seralini, G. E. Ethoxylated adjuvants of glyphosate-based herbicides are active principles of human cell toxicity. Toxicology 313, 122–128. https://doi.org/10.1016/j.tox.2012.09.006 (2013).CAS 
Article 
PubMed 

Google Scholar 
Chlopecka, M., Mendel, M., Dziekan, N. & Karlik, W. The effect of glyphosate-based herbicide Roundup and its co-formulant, POEA, on the motoric activity of rat intestine—In vitro study. Environ. Toxicol. Pharmacol. 49, 156–162. https://doi.org/10.1016/j.etap.2016.12.010 (2017).CAS 
Article 
PubMed 

Google Scholar 
Authority, E. F. S. Request for the evaluation of the toxicological assessment of the co-formulant POE-tallowamine. EFSA J. 13, 4303 (2015).
Google Scholar 
Bolognesi, C. et al. Genotoxic activity of glyphosate and its technical formulation Roundup. J. Agric. Food Chem. 45, 1957–1962 (1997).CAS 
Article 

Google Scholar 
Hao, Y. et al. Roundup((R)) confers cytotoxicity through DNA damage and mitochondria-associated apoptosis induction. Environ. Pollut. 252, 917–923. https://doi.org/10.1016/j.envpol.2019.05.128 (2019).CAS 
Article 
PubMed 

Google Scholar 
Luo, L. et al. In vitro cytotoxicity assessment of roundup (glyphosate) in L-02 hepatocytes. J. Environ. Sci. Health B 52, 410–417. https://doi.org/10.1080/03601234.2017.1293449 (2017).CAS 
Article 
PubMed 

Google Scholar 
Young, F., Ho, D., Glynn, D. & Edwards, V. Endocrine disruption and cytotoxicity of glyphosate and roundup in human JAr cells in vitro. Synthesis 14, 17 (2015).
Google Scholar 
Weinhold, B. Mystery in a bottle: Will the EPA require public disclosure of inert pesticide ingredients?. Environ. Health Perspect. 118, A168-171. https://doi.org/10.1289/ehp.118-a168 (2010).Article 
PubMed 
PubMed Central 

Google Scholar 
Richmond, M. E. Glyphosate: A review of its global use, environmental impact, and potential health effects on humans and other species. J. Environ. Stud. Sci. 8, 416–434 (2018).Article 

Google Scholar 
Cole, R. D., Anderson, G. L. & Williams, P. L. The nematode Caenorhabditis elegans as a model of organophosphate-induced mammalian neurotoxicity. Toxicol. Appl. Pharmacol. 194, 248–256. https://doi.org/10.1016/j.taap.2003.09.013 (2004).CAS 
Article 
PubMed 

Google Scholar 
Lai, C. H., Chou, C. Y., Ch’ang, L. Y., Liu, C. S. & Lin, W. Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics. Genome Res. 10, 703–713. https://doi.org/10.1101/gr.10.5.703 (2000).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Negga, R. et al. Exposure to glyphosate- and/or Mn/Zn-ethylene-bis-dithiocarbamate-containing pesticides leads to degeneration of gamma-aminobutyric acid and dopamine neurons in Caenorhabditis elegans. Neurotox. Res. 21, 281–290. https://doi.org/10.1007/s12640-011-9274-7 (2012).CAS 
Article 
PubMed 

Google Scholar 
Negga, R. et al. Exposure to Mn/Zn ethylene-bis-dithiocarbamate and glyphosate pesticides leads to neurodegeneration in Caenorhabditis elegans. Neurotoxicology 32, 331–341. https://doi.org/10.1016/j.neuro.2011.02.002 (2011).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Schuske, K., Beg, A. A. & Jorgensen, E. M. The GABA nervous system in C. elegans. Trends Neurosci. 27, 407–414. https://doi.org/10.1016/j.tins.2004.05.005 (2004).CAS 
Article 
PubMed 

Google Scholar 
McIntire, S. L., Jorgensen, E. & Horvitz, H. R. Genes required for GABA function in Caenorhabditis elegans. Nature 364, 334–337. https://doi.org/10.1038/364334a0 (1993).ADS 
CAS 
Article 
PubMed 

Google Scholar 
Eastman, C., Horvitz, H. R. & Jin, Y. Coordinated transcriptional regulation of the unc-25 glutamic acid decarboxylase and the unc-47 GABA vesicular transporter by the Caenorhabditis elegans UNC-30 homeodomain protein. J. Neurosci. 19, 6225–6234 (1999).CAS 
Article 

Google Scholar 
Bamber, B. A., Beg, A. A., Twyman, R. E. & Jorgensen, E. M. The Caenorhabditis elegans unc-49 locus encodes multiple subunits of a heteromultimeric GABA receptor. J. Neurosci. 19, 5348–5359 (1999).CAS 
Article 

Google Scholar 
Risley, M. G., Kelly, S. P., Jia, K., Grill, B. & Dawson-Scully, K. Modulating behavior in C. elegans using electroshock and antiepileptic drugs. PLoS ONE 11, e0163786. https://doi.org/10.1371/journal.pone.0163786 (2016).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Pandey, R. et al. Baccoside A suppresses epileptic-like seizure/convulsion in Caenorhabditis elegans. Seizure 19, 439–442. https://doi.org/10.1016/j.seizure.2010.06.005 (2010).Article 
PubMed 

Google Scholar 
Risley, M. G., Kelly, S. P. & Dawson-Scully, K. Electroshock induced seizures in adult C. elegans. Bio-Protoc. 7, 163786 (2017).Article 

Google Scholar 
Risley, M. G., Kelly, S. P., Minnerly, J., Jia, K. & Dawson-Scully, K. egl-4 modulates electroconvulsive seizure duration in C. elegans. Invert. Neurosci. 18, 8. https://doi.org/10.1007/s10158-018-0211-9 (2018).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
McVey, K. A. et al. Exposure of C. elegans eggs to a glyphosate-containing herbicide leads to abnormal neuronal morphology. Neurotoxicol. Teratol. 55, 23–31. https://doi.org/10.1016/j.ntt.2016.03.002 (2016).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Burchfield, S. L. et al. Acute exposure to a glyphosate-containing herbicide formulation inhibits Complex II and increases hydrogen peroxide in the model organism Caenorhabditis elegans. Environ. Toxicol. Pharmacol. 66, 36–42. https://doi.org/10.1016/j.etap.2018.12.019 (2019).CAS 
Article 
PubMed 

Google Scholar 
Weisskopf, M. G., Moisan, F., Tzourio, C., Rathouz, P. J. & Elbaz, A. Pesticide exposure and depression among agricultural workers in France. Am. J. Epidemiol. 178, 1051–1058. https://doi.org/10.1093/aje/kwt089 (2013).Article 
PubMed 

Google Scholar 
Kamel, F. et al. Pesticide exposure and self-reported Parkinson’s disease in the agricultural health study. Am. J. Epidemiol. 165, 364–374. https://doi.org/10.1093/aje/kwk024 (2007).CAS 
Article 
PubMed 

Google Scholar 
Tanner, C. M. Advances in environmental epidemiology. Mov. Disord. 25(Suppl 1), S58-62. https://doi.org/10.1002/mds.22721 (2010).Article 
PubMed 

Google Scholar 
Dick, F. D. Parkinson’s disease and pesticide exposures. Br. Med. Bull. 79–80, 219–231. https://doi.org/10.1093/bmb/ldl018 (2006).CAS 
Article 
PubMed 

Google Scholar 
Brown, T. P., Rumsby, P. C., Capleton, A. C., Rushton, L. & Levy, L. S. Pesticides and Parkinson’s disease–Is there a link?. Environ. Health Perspect. 114, 156–164. https://doi.org/10.1289/ehp.8095 (2006).Article 
PubMed 

Google Scholar 
Firestone, J. A. et al. Pesticides and risk of Parkinson disease: A population-based case-control study. Arch. Neurol. 62, 91–95. https://doi.org/10.1001/archneur.62.1.91 (2005).Article 
PubMed 

Google Scholar 
Martinez, M. A. et al. Neurotransmitter changes in rat brain regions following glyphosate exposure. Environ. Res. 161, 212–219. https://doi.org/10.1016/j.envres.2017.10.051 (2018).CAS 
Article 
PubMed 

Google Scholar 
Kalueff, A. V. & Nutt, D. J. Role of GABA in anxiety and depression. Depress. Anxiety 24, 495–517. https://doi.org/10.1002/da.20262 (2007).CAS 
Article 
PubMed 

Google Scholar 
Mohler, H. The GABA system in anxiety and depression and its therapeutic potential. Neuropharmacology 62, 42–53. https://doi.org/10.1016/j.neuropharm.2011.08.040 (2012).CAS 
Article 
PubMed 

Google Scholar 
Brambilla, P., Perez, J., Barale, F., Schettini, G. & Soares, J. C. GABAergic dysfunction in mood disorders. Mol. Psychiatry 8, 721–737. https://doi.org/10.1038/sj.mp.4001362 (2003) (715).CAS 
Article 
PubMed 

Google Scholar 
Xia, G. et al. Reciprocal control of obesity and anxiety-depressive disorder via a GABA and serotonin neural circuit. Mol. Psychiatry 26, 2837–2853. https://doi.org/10.1038/s41380-021-01053-w (2021).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Martinez, A. & Al-Ahmad, A. J. Effects of glyphosate and aminomethylphosphonic acid on an isogeneic model of the human blood-brain barrier. Toxicol. Lett. 304, 39–49. https://doi.org/10.1016/j.toxlet.2018.12.013 (2019).CAS 
Article 
PubMed 

Google Scholar 
Goetz, T., Arslan, A., Wisden, W. & Wulff, P. GABA(A) receptors: Structure and function in the basal ganglia. Prog. Brain Res. 160, 21–41. https://doi.org/10.1016/S0079-6123(06)60003-4 (2007).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Shaw, W. Elevated urinary glyphosate and clostridia metabolites with altered dopamine metabolism in triplets with autistic spectrum disorder or suspected seizure disorder: A case study. Integr. Med. (Encinitas) 16, 50–57 (2017).
Google Scholar 
Gaupp-Berghausen, M., Hofer, M., Rewald, B. & Zaller, J. G. Glyphosate-based herbicides reduce the activity and reproduction of earthworms and lead to increased soil nutrient concentrations. Sci. Rep. 5, 12886. https://doi.org/10.1038/srep12886 (2015).ADS 
CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Kanabar, M. et al. Roundup negatively impacts the behavior and nerve function of the Madagascar hissing cockroach (Gromphadorhina portentosa). Environ. Sci. Pollut. Res. Int. https://doi.org/10.1007/s11356-021-13021-6 (2021).Article 
PubMed 

Google Scholar 
Loscher, W., Fassbender, C. P. & Nolting, B. The role of technical, biological and pharmacological factors in the laboratory evaluation of anticonvulsant drugs. II. Maximal electroshock seizure models. Epilepsy Res. 8, 79–94. https://doi.org/10.1016/0920-1211(91)90075-q (1991).CAS 
Article 
PubMed 

Google Scholar 
Castel-Branco, M. M., Alves, G. L., Figueiredo, I. V., Falcao, A. C. & Caramona, M. M. The maximal electroshock seizure (MES) model in the preclinical assessment of potential new antiepileptic drugs. Methods Find. Exp. Clin. Pharmacol. 31, 101–106. https://doi.org/10.1358/mf.2009.31.2.1338414 (2009).CAS 
Article 
PubMed 

Google Scholar 
Luszczki, J. J. et al. Anticonvulsant and acute neurotoxic effects of imperatorin, osthole and valproate in the maximal electroshock seizure and chimney tests in mice: A comparative study. Epilepsy Res. 85, 293–299. https://doi.org/10.1016/j.eplepsyres.2009.03.027 (2009).CAS 
Article 
PubMed 

Google Scholar 
Suthakaran, N. et al. O-GlcNAc transferase OGT-1 and the ubiquitin ligase EEL-1 modulate seizure susceptibility in C. elegans. PLoS ONE 16, e0260072. https://doi.org/10.1371/journal.pone.0260072 (2021).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Hedberg, D. & Wallin, M. Effects of Roundup and glyphosate formulations on intracellular transport, microtubules and actin filaments in Xenopus laevis melanophores. Toxicol. In Vitro 24, 795–802. https://doi.org/10.1016/j.tiv.2009.12.020 (2010).CAS 
Article 
PubMed 

Google Scholar  More

Bromley, R. G. & Gale, A. S. The lithostratigraphy of the English Chalk Rock. Cretac. Res. 3, 273–306 (1982).Article 

Google Scholar 
Scholle, P. A., Arthur, M. A. & Ekdale, A. A. Pelagic environment. In Carbonate Depositional Environments (eds Scholle, P. A. et al.) 619–691 (Am. Ass. Petrol. Geol. Mem. 33, 1983).Chapter 

Google Scholar 
Gealy, E. L., Winterer, E. L. & Moberly, R. Methods, conventions, and general observations. Initial Rep. Deep Sea Drill. Proj. 7, 9–26 (1971).
Google Scholar 
Kroenke, L. W. et al. Ocean Drilling Program. Proc. ODP, Init. Repts. 130, College Station (1991).Dunham, R. L. Classification of carbonate rocks according to depositional texture. Mem. Am. Assoc. Petrol. Geol. 1, 108–121 (1962).
Google Scholar 
Quine, M. & Bosence, D. Stratal geometries, facies and sea-floor erosion in Upper Cretaceous chalk, Normandy, France. Sedimentology 38, 1113–1152 (1991).ADS 
Article 

Google Scholar 
Røgen, B., Gommesen, L. & Fabricius, I. L. Grain size distributions of Chalk from Image analysis of electron micrographs. Comput. Geosci. 27, 1071–1080 (2001).ADS 
Article 

Google Scholar 
Saïag, J. et al. Classifying chalk microtextures: Sedimentary versus diagenetic origin (Cenomanian–Santonian, Paris Basin, France). Sedimentology 66, 2976–3007 (2019).Article 
CAS 

Google Scholar 
Scholle, P. A. Chalk diagenesis and its relation to petroleum exploration: Oil from chalks, a modern miracle?. Bull. Am. Assoc. Petrol. Geol. 61, 982–1009 (1977).CAS 

Google Scholar 
Tagliavento, M., John, C. M., Anderskouv, K. & Stemmerik, L. Towards a new understanding of the genesis of chalk: Diagenetic origin of micarbs confirmed by clumped isotope analysis. Sedimentology 68, 513–530 (2021).CAS 
Article 

Google Scholar 
Bramlette, M. N. Significance of coccolithophorids in calcium-carbonate deposition. Bull. Geol. Soc. Am. 69, 121–126 (1958).Article 

Google Scholar 
Hattin, D. E. & Darko, D. A. Technique for determining coccolith abundance in shaly chalk of Greenhorn Limestone (Upper Cretaceous) of Kansas. Kansas Geol. Surv. Bull. 202, 1–11 (1971).
Google Scholar 
Houghton, S. D. Calcareous nannofossils. In Calcareous algae and Stromatolites (ed. Riding, R.) 217–266 (Springer, 1991).Chapter 

Google Scholar 
Bown, P. R., Lees, J. A. & Young, J. R. Calcareous nannoplankton evolution and diversity through time. In Coccolithophores—From Molecular Processes to Global Impact (eds Thierstein, H. R. & Young, J. R.) 481–508 (Springer, 2004).
Google Scholar 
Roth, P. H. Mesozoic paleoceanography of the North Atlantic and Tethys Oceans. In North Atlantic Paleoceanography (eds Summerhayes, C. P. & Shackleton, N. J.) 299–320 (Geological Society Special Publications, 1986).
Google Scholar 
Baumann, K.-H., Andruleit, H., Böckel, B., Geisen, M. & Kinkel, H. The significance of extant coccolithophores as indicators of ocean water masses, surface water temperature, and paleoproductivity: A review. Paläontol. Z. 79, 93–112 (2005).Article 

Google Scholar 
Miller, K. G. et al. The phanerozoic record of global sea-level change. Science 310, 1293–1298 (2005).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Ando, A., Huber, B. T., MacLeod, K. G. & Watkins, D. K. Early Cenomanian “hot greenhouse” revealed by oxygen isotope record of exceptionally well-preserved foraminifera from Tanzania. Paleoceanography 30, 1556–1572 (2015).ADS 
Article 

Google Scholar 
Ekdale, A. A. & Bromley, R. G. Comparative ichnology of shelf-sea and deep-sea chalk. J. Paleontol. 58, 322–332 (1984).
Google Scholar 
Savrda, C. E. Chalk and related deep-marine carbonates. In Trace Fossils as Indicators of Sedimentary Environments (eds Knaust, D. & Bromley, R. G.) 777–806 (Elsevier, 2012).Chapter 

Google Scholar 
Savrda, C. E., Foster, C. & Fluegeman, R. A unique Lower Paleocene shelf-sea chalk in the eastern U.S. Gulf coastal plain (Clayton Formation, western Alabama): Implications for depositional environment, sea-level dynamics and paleogeography. Palaeogeogr. Palaeoclimatol. Palaeoecol. 538, 109439 (2020).Article 

Google Scholar 
Erba, E., Watkins, D. & Mutterlose, J. Campanian dwarf calcareous nannofossils from Wodejebato Guyot. In Proc. Ocean Drill. Program Sci. Results (eds Haggerty, J. A. et al.) 141–155 (Ocean Drilling Program, 1995).
Google Scholar 
Hancock, J. M. The petrology of chalk. Proc. Geol. Assoc. 86, 499–535 (1975).Article 

Google Scholar 
Stanley, S. M. & Hardie, L. A. Secular oscillations in the carbonate mineralogy of reef-building and sediment-producing organisms driven by tectonically forced shifts in seawater chemistry. Palaeogeogr. Palaeoclimatol. Palaeoecol. 144, 3–19 (1998).Article 

Google Scholar 
Stanley, S. M., Ries, J. B. & Hardie, L. A. Seawater chemistry, coccolithophore population growth, and the origin of Cretaceous chalk. Geology 33, 593–596 (2005).ADS 
CAS 
Article 

Google Scholar 
Pemberton, S. G. et al. Ichnology and sedimentology of shallow to marginal marine systems: Ben Nevis and Avalon Reservoirs, Jeanne d’Arc Basin. Geol. Assoc. Can. Short Course Notes 15, 1–343 (2001).
Google Scholar 
Buatois, L. A. & Mángano, M. G. Ichnology: Organism–Substrate Interactions in Space and Time (Cambridge Press University, 2011).Book 

Google Scholar 
Frey, R. W. & Bromley, R. G. Ichnology of American chalks: The Selma Group (Upper Cretaceous), western Alabama. Can. J. Earth Sci. 22, 801–828 (1985).ADS 
Article 

Google Scholar 
Savrda, C. E. & Bottjer, D. Trace-fossil model for reconstructing oxygenation histories of ancient marine bottom waters: Application to Upper Cretaceous Niobrara Formation, Colorado. Palaeogeogr. Palaeoclimatol. Palaeoecol. 74, 49–74 (1989).Article 

Google Scholar 
Kennedy, W. J. Trace fossils in carbonate rocks. In The Study of Trace Fossils (ed. Frey, R. W.) 377–398 (Springer, 1975).Chapter 

Google Scholar 
Loucks, R. G., Gates, B. G. & Zahm, C. K. Depositional systems, lithofacies, nanopore to micropore matrix network, and reservoir quality of the Upper Cretaceous (Cenomanian) Buda Limestone in Dimmit County, southwestern Texas. Gulf Coast Assoc. Geol. Soc. 8, 281–300 (2019).
Google Scholar 
Valencia, F. L. et al. Depositional environments and controls on the stratigraphic architecture of the Cenomanian Buda Limestone in west Texas, U.S.A. Mar. Petrol. Geol. 133, 105275 (2021).Article 

Google Scholar 
Valencia, F. L., Laya, J. C., Buatois, L. A., Mángano, M. G. & Valencia, G. L. Sedimentology and stratigraphy of the Cenomanian Buda Limestone in central Texas, U.S.A.: Implications on regional and global depositional controls. Cretac. Res. 137, 105231 (2022).Article 

Google Scholar 
Martin, K. G. Stratigraphy of the Buda Limestone, south-central Texas. In Comanchean (Lower Cretaceous) Stratigraphy and Paleontology of Texas (ed. Hendricks, L.) 287–299 (Permian Basin Section SEPM 67 (8), 1967).
Google Scholar 
Mallon, A. J. & Swarbrick, R. E. Diagenetic characteristics of low permeability, non-reservoir chalks from the Central North Sea. Mar. Petrol. Geol. 25, 1097–1108 (2008).CAS 
Article 

Google Scholar 
Brasher, J. E. & Vagle, K. R. Influence of lithofacies and diagenesis on Norwegian North Sea chalk reservoirs. Am. Assoc. Petrol. Geol. Bull. 80, 746–769 (1996).CAS 

Google Scholar 
Hentz, T. F. & Ruppel, S. C. Regional stratigraphic and rock characteristics of eagle ford shale in its play area: Maverick Basin to East Texas Basin. Am. Ass. Petrol. Geol. Search and Discovery 10325 (2011).Robinson, W. C. Petrography and depositional environments of the Buda Limestone, northern Coahuila, Mexico. MS Thesis. The University of Texas, 156 (1982).Reaser, D. F. & Robinson, W. C. Cretaceous Buda Limestone in west Texas and northern Mexico. In Cretaceous Stratigraphy and Paleoecology, Texas and Mexico (ed. Scott, R. W.) 337–373 (Perkins Memorial volume, GCSSEPM Foundation, Special Publications in Geology 1, 2003).
Google Scholar 
Young, K. P. Cretaceous paleogeography: Implications of endemic ammonite faunas. Geol. Circ. (University of Texas at Austin, Bureau of Economic Geology) 72, 1–13 (1972).
Google Scholar 
Buatois, L. A. & Mángano, M. G. Ichnodiversity and ichnodisparity: Significance and caveats. Lethaia 46, 281–292 (2013).Article 

Google Scholar 
Buatois, L. A., Wisshak, M., Wilson, M. A. & Mángano, M. G. Categories of architectural designs in trace fossils: A measure of ichnodisparity. Earth Sci. Rev. 164, 102–181 (2017).ADS 
CAS 
Article 

Google Scholar 
Swinbanks, D. D. & Luternauer, J. L. Burrow distribution of thalassinidean shrimp on a Fraser Delta tidal flat, British Columbia. J. Paleontol. 61, 315–333 (1987).Article 

Google Scholar 
Carmona, N. B., Buatois, L. A. & Mángano, M. G. The trace fossil record of burrowing decapod crustaceans: Evaluating evolutionary radiations and behavioural convergence. In Trace Fossils in Evolutionary Palaeoecology (eds Webby, B. D. et al.) 141–153 (Wiley, 2004).
Google Scholar 
Baucon, A. et al. Ethology of the trace fossil Chondrites: Form, function and environment. Earth Sci. Rev. 202, 102989 (2020).CAS 
Article 

Google Scholar 
Pemberton, S. G. & Frey, R. W. Trace fossil nomenclature and the Planolites–Palaeophycus dilemma. J. Paleontol. 56, 843–881 (1982).
Google Scholar 
Rodríguez-Tovar, F. J. & Pérez-Valera, F. Trace fossil Rhizocorallium from the Middle Triassic of the Betic Cordillera, Southern Spain: Characterization and environmental implications. Palaios 23, 78–86 (2008).ADS 
Article 

Google Scholar 
Bown, T. M. & Kraus, M. J. Ichnofossils of the alluvial Willwood Formation (lower Eocene), Bighorn Basin, northwest Wyoming, USA. Palaeogeogr. Palaeoclimatol. Palaeoecol 43, 95–128 (1983).Article 

Google Scholar 
Uchman, A. Taxonomy and palaeoecology of flysch trace fossils: The Marnoso-arenacea Formation and associated facies (Miocene, Northern Apennines, Italy). Beringeria 15, 3–115 (1995).
Google Scholar 
Demírcan, H. & Uchman, A. The miniature trace fossil Bichordites kuzunensis isp. Nov., from early Oligocene prodelta sediments of the Mezardere Formation, Gökçeada Island, NW Turkey. Acta Geol. Pol. 62, 205–215 (2012).
Google Scholar 
Plaziat, J.-C. & Mahmoudi, M. Trace fossils attributed to burrowing echinoids: A revision including new ichnogenus and ichnospecies. Geobios 21, 209–233 (1988).Article 

Google Scholar 
Chamberlain, C. K. Morphology and ethology of trace fossils from the Ouachita Mountains, southeast Oklahoma. J. Paleontol. 45, 212–246 (1971).
Google Scholar 
Farrow, G. E. Bathymetric zonation of Jurassic trace fossils from the coast of Yorkshire, England. Palaeogeogr. Palaeoclimatol. Palaeoecol. 2, 103–151 (1966).Article 

Google Scholar 
Mángano, M. G., Buatois, L. A., West, R. R. & Maples, C. G. Contrasting behavioral and feeding strategies recorded by tidal-flat bivalve trace fossils from the upper carboniferous of eastern Kansas. Palaios 13, 335–351 (1998).ADS 
Article 

Google Scholar 
Pemberton, S. G., Frey, R. W. & Bromley, R. G. The ichnotaxonomy of Conostichus and other plug-shaped ichnofossils. Can. J. Earth Sci. 25, 866–892 (1988).ADS 
Article 

Google Scholar 
Nara, M. Rosselia socialis: A dwelling structure of a probable terebellid polychaete. Lethaia 28, 171–178 (1995).Article 

Google Scholar 
Wilson, M. A., Curran, H. A. & White, B. Paleontological evidence of a brief global sea-level event during the last interglacial. Lethaia 31, 241–250 (1998).Article 

Google Scholar 
Santos, A., Mayoral, E., Marques da Silva, C., Cachão, M. & Kullberg, J. C. Trypanites ichnofacies: Palaeoenvironmental and tectonic implications. A case study from the Miocene disconformity at Foz da Fonte (Lower Tagus Basin, Portugal). Palaeogeogr. Palaeoclimatol. Palaeoecol. 292, 35–43 (2010).Article 

Google Scholar 
Wilson, J. L. Carbonate Facies in Geological History (Springer, 1975).Book 

Google Scholar 
Tucker, M. E. & Wright, V. P. Carbonate Sedimentology (Blackwell Science, 1990).Book 

Google Scholar 
MacEachern, J. A. & Gingras, M. K. Recognition of brackish-water trace fossil assemblages in the Cretaceous western interior seaway of Alberta. In Sediment-Organism Interactions: A Multifaceted Ichnology (eds Bromley, R. G. et al.) 149–194 (Society for Sedimentary Geology Special Publication, 2007).
Google Scholar 
MacEachern, J. A., Zaitlin, B. A. & Pemberton, S. G. High-resolution sequence stratigraphy of early transgressive deposits, Viking Formation, Joffre Field, Alberta, Canada. Bull. Am. Assoc. Petrol. Geol. 82, 729–756 (1998).
Google Scholar 
Buatois, L. A., Netto, R. G. & Mángano, M. G. Ichnology of Permian marginal-marine to shallow-marine coal-bearing successions: Rio Bonito and Palermo formations, Parana Basin, Brazil. In Applied Ichnology (eds MacEachern, J. A. et al.) 167–177 (Society for Sedimentary Geology Short Course Notes, 2007).
Google Scholar 
Buatois, L. A. et al. Colonization of brackish-water systems through time: Evidence from the trace-fossil record. Palaios 20, 321–347 (2005).ADS 
Article 

Google Scholar 
Pemberton, S. G. & Wightman, D. M. Ichnological characteristics of brackish water deposits. In Applications of Ichnology to Petroleum Exploration: A Core Work-shop (ed. Pemberton, S. G.) 141–167 (Society of Economic Paleontologists and Mineralogists Core Workshop, 1992).Chapter 

Google Scholar 
Anderson, B. G. & Droser, M. L. Ichnofabrics and geometric configurations of Ophiomorpha within a sequence stratigraphic framework: An example from the Upper Cretaceous US western interior. Sedimentology 45, 379–396 (1998).ADS 
Article 

Google Scholar 
Buatois, L. A., Mángano, M. G. & Pattison, S. A. J. Ichnology of prodeltaic hyperpycnite–turbidite channel complexes and lobes from the Upper Cretaceous Prairie Canyon Member of the Mancos Shale, Book Cliffs, Utah, USA. Sedimentology 66, 1825–1860 (2019).Article 

Google Scholar 
Bhattacharya, J. P. & MacEachern, J. A. Hyperpycnal rivers and prodeltaic shelves in the Cretaceous seaway of North America. J. Sediment. Res. 79, 184–209 (2009).ADS 
Article 

Google Scholar 
Savrda, C. E. Ichnosedimentologic evidence for a noncatastrophic origin of Cretaceous-Tertiary boundary sand in Alabama. Geology 21, 1075–1078 (1993).ADS 
Article 

Google Scholar 
Schlager, W. Accommodation and supply-a dual control on stratigraphic sequences. Sediment. Geol. 86, 111–136 (1993).ADS 
Article 

Google Scholar 
Strasser, A. & Samankassou, E. Carbonate sedimentation rates today and in the past: Holocene of Florida Bay, Bahamas, and Bermuda vs. Upper Jurassic and Lower Cretaceous of the Jura Mountains (Switzerland and France). Geol. Croat. 56, 1–18 (2003).Article 

Google Scholar 
Moyano-Paz, D., Richiano, S., Varela, A. N., Gómez-Dacal, A. R. & Poire, D. G. Ichnological signatures from wave- and fluvial-dominated deltas: The La Anita Fromation, Upper Cretaceous, Austral-Magallanes Basin, Patagonia. Mar. Pet. Geol. 114, 104168 (2020).CAS 
Article 

Google Scholar 
De Gibert, J. M. & Ekdale, A. A. Trace fossil assemblages reflecting stressed environments in the Middle Jurassic Carmel Seaway of Central Utah. J. Paleontol. 73, 711–720 (1999).Article 

Google Scholar 
Gingras, M. K., MacEachern, J. A. & Dashtgard, S. E. Process ichnology and the elucidation of physico-chemical stress. Sediment. Geol. 237, 115–134 (2011).ADS 
CAS 
Article 

Google Scholar 
Smith, C. R., Levin, L. A., Hoover, D. J., McMurty, G. & Gage, J. D. Variations in bioturbation across the oxygen minimum zone in the northwest Arabian Sea. Deep-Sea Res. II 47, 227–257 (2000).ADS 
CAS 
Article 

Google Scholar 
Wignall, P. B., Newton, R. & Brookfield, M. E. Pyrite framboid evidence for oxygen-poor deposition during the Permian-Triassic crisis in Kashmir. Palaeogeogr. Palaeoclimatol. Palaeoecol. 216, 183–188 (2005).Article 

Google Scholar 
Kennedy, W. J. Burrows and surface traces from the Lower Chalk of southern England. Bull. Br. Mus. Nat. Hist. Geol. 15, 127–167 (1967).
Google Scholar 
Kennedy, W. J. & Garrison, R. E. Morphology and genesis of nodular chalks and hardgrounds in the Upper Cretaceous of southern England. Sedimentology 22, 311–386 (1975).ADS 
CAS 
Article 

Google Scholar 
Bromley, R. G. Some observations on burrows of thalassinidean Crustacea in chalk hardgrounds. Geol. Soc. Lond. Q. J. 123, 157–182 (1967).Article 

Google Scholar 
Bromley, R. G. Trace fossils at omission surfaces. In The Study of Trace Fossils (ed. Frey, R. W.) 399–428 (Springer, 1975).Chapter 

Google Scholar 
Hart, M. B., Harries, P. J. & Cárdenas, A. L. The Cretaceous/Paleogene boundary events in the Gulf Coast: Comparisons between Alabama and Texas. Gulf Coast Assoc. Geol. Trans. 63, 235–255 (2013).
Google Scholar 
Al Balushi, S. A. K. & Macquaker, J. H. S. Sedimentological evidence for bottom-water oxygenation during deposition of the Natih-B Member intrashelf-basinal sediments: Upper Cretaceous carbonate source rock, Natih Formation, North Sultanate of Oman. GeoArabia 16, 47–84 (2011).Article 

Google Scholar 
Lasseur, E. et al. A relative water-depth model for the Normandy Chalk (Cenomanian–Middle Coniacian, Paris Basin, France) based on facies patterns of metre-scale cycles. Sediment. Geol. 213, 1–26 (2009).ADS 
Article 

Google Scholar 
Dawson, W. C. & Reaser, D. F. Rhizocorallium in the upper Austin Chalk, Ellis County, Texas. Texas J. of Sci. 23, 207–214 (1980).
Google Scholar 
Dawson, W. C. & Reaser, D. F. Ichnology and paleoenvironments of the middle and upper Austin Chalk (Upper Cretaceous), northeastern Texas. Trans. Am. Assoc. Pet. Geol. Southwest Sec. 1985, 47–67 (1985).
Google Scholar 
Dawson, W. C. & Reaser, D. F. Trace fossils and paleoenvironments of lower and middle Austin Chalk (Upper Cretaceous), north-central Texas. Trans. Gulf Coast Assoc. Geol. Soc. 40, 161–173 (1990).
Google Scholar 
Dawson, W. C. & Reaser, D. F. Ichnology and Paleosubstrates of Austin Chalk (Cretaceous) Outcrops: Southern Dallas and Ellis Counties, Texas. Am. Assoc. Pet. Geol. Search Discovery Article #91004 (1991).Fürsich, F. T., Kennedy, W. J. & Palmer, T. J. Trace fossils at a regional discontinuity surface: The Austin/Taylor (Upper Cretaceous) contact in central Texas. J. Paleontol. 55, 537–551 (1981).
Google Scholar 
Morgan, R. F. A new ichnospecies of Gyrolithes from the Austin Chalk, Upper Cretaceous, Texas, USA. Ichnos 26, 1–7 (2018).Article 

Google Scholar 
Cooper, J. R., Godet, A. & Pope, M. C. Tectonic and eustatic impact on depositional features in the upper Cretaceous Austin Chalk Group of south-central Texas, USA. Sediment. Geol. 401, 105632 (2020).Article 

Google Scholar 
Loucks, R. G. et al. Geologic characterization of the type cored section for the Upper Cretaceous Austin Chalk Group in southern Texas: A combination fractured and unconventional reservoir. Am. Assoc. Pet. Geol. Bull. 104, 2209–2245 (2020).
Google Scholar 
Loucks, R. G., Reed, R. M., Ko, L. T., Zahm, C. K. & Larson, T. E. Micropetrographic characterization of a siliciclastic-rich chalk; Upper Cretaceous Austin Chalk Group along the onshore northern Gulf of Mexico, USA. Sediment. Geol. 412, 105821 (2021).CAS 
Article 

Google Scholar 
Bottjer, D. J. Paleoecology, Ichnology, and Depositional Environments of Upper Cretaceous Chalks (Annona Formation; chalk Member of Saratoga Formation), Southwestern Arkansas. PhD Dissertation, Indiana University, 424 (1978).Bottjer, D. J. Ichnology and depositional environments of Upper Cretaceous chalks, southwestern Arkansas (Annona Formation; chalk member, Saratoga Formation). Am. Assoc. Pet. Geol. Bull. 63, 422 (1979).
Google Scholar 
Bottjer, D. J. Trace fossils and paleoenvironments of two Arkansas Upper Cretaceous discontinuity surfaces. J. Paleontol. 59, 282–298 (1985).
Google Scholar 
Bottjer, D. J. Campanian-Maastrichtian chalks of southwestern Arkansas: Petrology, paleoenvironments and comparison with other North American and European chalks. Cretac. Res. 7, 161–196 (1986).Article 

Google Scholar 
Bayet-Goll, A., Neto de Carvalho, C., Monaco, P. & Sharafi, M. Sequence stratigraphic and sedimentologic significance of biogenic structures from chalky limestones of the Turonian-Campanian Abderaz Formation, Kopet-Dagh, Iran. In Cretaceous Period: Biotic Diversity and Biogeography (eds Khosla, A. & Lucas, S. G.) 19–43 (New Mex. Mus. Nat. His. Sci. Bull. 71, 2016).
Google Scholar 
Locklair, R. E. & Savrda, C. E. Ichnology of rhythmically bedded Demopolis Chalk (Upper Cretaceous, Alabama): Implications for paleoenvironment, depositional cycle origins, and tracemaker behavior. Palaios 13, 423–438 (1998).ADS 
Article 

Google Scholar 
Locklair, R. E. & Savrda, C. E. Ichnofossil tiering analysis of a rhythmically bedded chalk-marl sequence in the Upper Cretaceous of Alabama. Lethaia 31, 311–322 (1998).Article 

Google Scholar 
Kennedy, W. J. Trace fossils in the chalk environment. In Trace Fossils (eds Crimes, T. P. & Harper, J. C.) 263–282 (Geological Journal Special Issue 3, 1970).
Google Scholar 
Mortimore, R. N. & Pomerol, B. Stratigraphy and eustatic implications of trace fossil events in the Upper Cretaceous Chalk of northern Europe. Palaios 6, 216–231 (1991).ADS 
Article 

Google Scholar 
Foster, C. B. III. Geology of the Moscow Landing Section, Tombigbee River, Western Alabama, with Focus on Ichnologic Aspects of the Lower Paleocene Clayton Formation. M.Sc. Dissertation, Auburn University, 88 (2019).Gabdullin, R. R. Rhythmicity of the Upper Cretaceous Deposits of the East European Craton, Northwestern Caucasus and Southwestern Crimea: Structure, Classification, Formation Models (Mosk. Gos. Univ., 2002).
Google Scholar 
Baraboshkin, E. Y. & Zibrov, I. A. Characteristics of the Middle Cenomanian Rhythmic Sequence from Mount Selbukhra in Southwest Crimea. Moscow Univ. Geol. Bull. 67, 176–184 (2012).Article 

Google Scholar 
Blinkenberg, K. H., Lauridsen, B. W., Knaust, D. & Stemmerik, L. New ichnofabrics of the Cenomanian-Danian Chalk Group. J. Sediment. Res. 90, 701–712 (2020).ADS 
Article 

Google Scholar 
Ekdale, A. A. & Bromley, R. G. Trace fossils and ichnofabric in the Kjolby Gaard Marl, uppermost Cretaceous, Denmark. Bull. Geol. Soc. Denmark 31, 107–119 (1983).Article 

Google Scholar 
Ekdale, A. A. & Bromley, R. G. Cretaceous chalk ichnofacies in northern Europe. Geobios 8, 201–204 (1984).Article 

Google Scholar 
Ekdale, A. A. & Bromley, R. G. Analysis of composite ichnofabrics; An example in Uppermost Cretaceous chalk of Denmark. Palaios 6, 232–249 (1991).ADS 
Article 

Google Scholar 
Surlyk, F. et al. The cyclic Rørdal Member—A new lithostratigraphic unit of chronostratigraphic and palaeoclimatic importance in the upper Maastrichtian of Denmark. Bull. Geol. Soc. Denmark 58, 89–98 (2010).Article 

Google Scholar 
Lauridsen, B. W., Surlyk, F. & Bromley, R. G. Trace fossils of a cyclic chalk marl succession; the upper Maastrichtian Rørdal Member, Denamrk. Cretac. Res. 32, 194–211 (2011).Article 

Google Scholar 
Frey, R. W. Trace fossils of Fort Hays Limestone Member of Niobrara Chalk (Upper Cretaceous), west-central Kansas. Univ. Kansas Paleontol. Contrib. 53, 52 (1970).
Google Scholar 
Hattin, D. E. Stratigraphy and depositional environment of Smoky Hill Chalk Member, Niobrara Chalk (Upper Cretaceous) of the type area western Kansas. Kansas Geol. Surv. Bull. 225, 1–108 (1982).
Google Scholar 
Savrda, C. E. Ichnocoenoses in the Niobrara Formation: Implications for benthic oxygenation histories. In Stratigraphy and Paleoenvironments of the Cretaceous Western Interior Seaway, USA (eds Dean, W. E. & Arthur, M. A.) 137–151 (SEPM Society for Sedimentary Geology 6, 1998).Chapter 

Google Scholar 
Hattin, D. E. Widespread, synchronously deposited, burrow-mottled limestone beds in Greenhorn Limestone (Upper Cretaceous) of Kansas and southeastern Colorado. Am. Assoc. Pet. Geol. Bull. 55, 412–431 (1971).
Google Scholar 
Hattin, D. E. Stratigraphy and depositional environment of Greenhorn Limestone (Upper Cretaceous) of Kansas. Kansas Geol. Surv. Bull. 209, 128 (1975).
Google Scholar 
Savrda, C. E. Ichnology of the Bridge Creek Limestone: Evidence for temporal and spatial variations in paleo-oxygenation in the Western Interior Seaway. In Stratigraphy and Paleoenvironments of the Cretaceous Western Interior Seaway, USA (eds Dean, W. E. & Arthur, M. A.) 127–136 (SEPM Society for Sedimentary Geology 6, 1998).Chapter 

Google Scholar 
Rasmussen, S. L. & Surlyk, F. Facies and ichnology of an Upper Cretaceous chalk contourite drift complex, eastern Denmark, and the validity of contourite facies models. J. Geol. Soc. Lond. 169, 435–447 (2012).Article 

Google Scholar 
Surlyk, F. et al. Upper Campanian-Maastrichtian holostratigraphy of the eastern Danish Basin. Cretac. Res. 46, 232–256 (2013).Article 

Google Scholar 
Boussaha, M., Thibault, N., Anderskouv, K., Moreau, J. & Stemmerik, L. Controls on upper Campanian-Maastrichtian chalk deposition in the eastern Danish Basin. Sedimentology 64, 1998–2030 (2017).Article 

Google Scholar 
Reolid, J. & Betzler, C. The ichnology of carbonate drifts. Sedimentology 66, 1427–1448 (2019).Article 

Google Scholar 
Nygaard, E. Bathichnus and Its Significance in the Trace Fossil Association of Upper Cretaceous Chalk, Mors, Denmark 107–113 (Danm. Geol. Unders. Årbog, 1983).
Google Scholar 
Scholle, P. A., Albrechtsen, T. & Tirsgaard, H. Formation and diagenesis of bedding cycles in uppermost Cretaceous chalks of the Dan Field, Danish North Sea. Sedimentology 45, 223–243 (1998).ADS 
CAS 
Article 

Google Scholar 
Damholt, T. & Surlyk, F. Laminated–bioturbated cycles in Maastrichtian chalk of the North Sea: Oxygenation fluctuations within the Milankovitch frequency band. Sedimentology 51, 1323–1342 (2004).ADS 
Article 

Google Scholar 
Anderskouv, K. & Surlyk, F. Upper Cretaceous chalk facies and depositional history recorded in the Mona-1 core, Mona Ridge, Danish North Sea. Geol. Surv. Denmark Greenland Bull. 25, 1–60 (2011).Article 

Google Scholar 
Maliva, R. G. & Dickson, J. A. D. Microfacies and diagenetic controls of porosity in Cretaceous/Tertiary chalks, Eldfisk Field, Norwegian North Sea. Am. Assoc. Pet. Geol. Bull. 76, 1825–1838 (1992).
Google Scholar 
Knaust, D., Dorador, J. & Rodríguez-Tovar, F. J. Burrowed matrix powering dual porosity systems—A case study from the Maastrichtian chalk of the Gullfaks Field Norwegian North Sea. Mar. Petrol. Geol. 113, 104158 (2020).Article 

Google Scholar 
Phillips, C. & McIlroy, D. Ichnofabrics and biologically mediated changes in clay mineral assemblages from a deep-water, fine-grained, calcareous sedimentary succession: An example from the Upper Cretaceous Wyandot Formation, offshore Nova Scotia. Bull. Can. Petrol. Geol. 58, 203–218 (2010).Article 

Google Scholar 
Rodríguez-Tovar, F. J. & Hernández-Molina, F. J. Ichnological analysis of contourites: Past, present and future. Earth-Sci. Rev. 182, 28–41 (2018).ADS 
Article 

Google Scholar 
Miguez-Salas, O. & Rodríguez-Tovar, F. J. Ichnofacies distribution in the Eocene-Early Miocene Petra Tou Romiou outcrop, Cyprus: Sea level dynamics and palaeoenvironmental implications in a contourite environment. Int. J. Earth Sci. 108, 2531–2544 (2019).CAS 
Article 

Google Scholar 
Nelson, C. S. Bioturbation in middle bathyal, Cenozoic nannofossil oozes and chalks, southwest Pacific. In Initial Reports of the Deep Sea Drilling Project 90 (eds Kennett, J. P., von der Borch, C. C. et al.) 1189–1200 (Washington U.S. Government Printing Office, 1986).
Google Scholar 
Fütterer, D. K. Bioturbation and trace fossils in deep sea sediments of the Walvis Ridge, southeastern Atlantic, Leg 74. In Initial Reports of the Deep Sea Drilling Project 74 (eds Moore, T. C., Rabinowitz, P. D. et al.) 543–555 (Government Printing Office, 1984).
Google Scholar 
Wetzel, A. Ichnofabrics in Eocene to Maestrichtian sediments from Deep Sea Drilling Project Site 605, off the New Jersey coast. In Initial Reports of the Deep Sea Drilling Project 93 (eds. Hinte, J. E., Wise Jr., S. W. et al.) 825–835 (1987).Droser, M. L. & Bottjer, D. J. Trace fossils and ichnofabrics in Leg 119 cores. In Proceedings of the Ocean Drilling Program, Scientific Results 119 (eds. Barron, J., Larsen, B. et al.) 635–641 (1991).Desai, B. G. Ichnofabric analysis of bathyal chalks: The Miocene Inglis Formation of the Andaman and Nicobar Islands, India. J. Palaeogeogr. 10, 1–15 (2021).Article 

Google Scholar 
Warme, J. E., Kennedy, W. J. & Scheidermann, N. Biogenic sedimentary structures (trace fossils) in Leg 15 cores. In Initial Reports of the Deep Sea Drilling Project 15 (eds. Edgar, N. T., Saunders, J. B. et al.) 813–831 (1973).Maurrasse, F. Sedimentary structures of Caribbean Leg 15 sediments. In Initial Reports of the Deep-Sea Drilling Project 15 (eds. Edgar, T. et al.) (1974).Erba, E. & Premoli-Silva, I. Orbitally driven cycles in trace-fossil distribution from the Piobbico core (late Albian, central Italy). In Orbital Forcing and Cyclic Sequences, IAS Spec. Publ. 19 (eds De Boer, P. L. & Smith, D. G.) 211–225 (Blackwell Scientific, 1994).
Google Scholar 
Chamberlain, C. K. Trace fossils in DSDP cores of the Pacific. J. Paleontol. 49, 1074–1096 (1975).
Google Scholar 
Ekdale, A. A. Trace fossils in Deep Sea Drilling Project Leg 58 cores. In Initial Reports of the Deep Sea Drilling Project 58 (eds. de Vries Klein, G., Kobyashi, K. et al.) 601–605 (1980).Ekdale, A. A. Geologic history of the abyssal benthos: Evidence from trace fossils in Deep-Sea Drilling Project cores. PhD Dissertation, Rice University, 154 (1974).Ekdale, A. A. Abyssal trace fossils in worldwide Deep Sea Drilling Project cores. In Trace Fossils 2 (eds. Crimes, T. P. & Harper, J. C.) 163–182 (Geol. J., Spec. Iss. 9, 1977).Ekdale, A. A. & Berger, W. H. Deep-sea ichnofacies: Modern organism traces on and in pelagic carbonates of the western equatorial Pacific. Palaeogeogr. Palaeoclimatol. Palaeoecol. 23, 263–278 (1978).Article 

Google Scholar 
Ekdale, A. A., Muller, L. N. & Novak, M. T. Quantitative ichnology of modern pelagic deposits in the abyssal Atlantic. Palaeogeogr. Palaeoclimatol. Palaeoecol. 45, 189–223 (1984).CAS 
Article 

Google Scholar 
Savrda, C. E. Limited ichnologic fidelity and temporal resolution in pelagic sediments: Paleoenvironmental and paleoecologic implications. Palaios 29, 210–217 (2014).ADS 
Article 

Google Scholar 
Bromley, R. G. & Ekdale, A. A. Composite ichnofabrics and tiering of burrows. Geol. Mag. 123, 59–65 (1986).ADS 
Article 

Google Scholar 
Griffin, J. N. et al. Spatial heterogeneity increases the importance of species richness for an ecosystem process. Oikos 118, 1335–1342 (2009).Article 

Google Scholar 
Valentine, J. W. Overview of marine biodiversity. In Marine Macroecology (eds Witman, J. D. & Roy, K.) 3–28 (University of Chicago Press, 2009).Chapter 

Google Scholar 
Schlacher, T. A. et al. Soft-sediment benthic community structure in a coral reef lagoon—The prominence of spatial heterogeneity and “spot endemism”. Mar. Ecol. Prog. Ser. 174, 159–174 (1998).ADS 
Article 

Google Scholar 
Hummel, H. et al. Geographic patterns of biodiversity in European coastal marine benthos. J. Mar. Biol. Assoc. U.K. 97, 507–523 (2017).Article 

Google Scholar 
Harborne, A. R., Mumby, P. J., Żychaluk, K., Hedley, J. D. & Blackwell, P. G. Modeling the beta diversity of coral reefs. Ecology 87, 2871–2881 (2006).PubMed 
Article 

Google Scholar 
Christia, C., Giordani, G. & Papastergiadou, E. Environmental variability and macrophyte assemblages in coastal lagoon types of Western Greece (Mediterranean Sea). Water 10, 151 (2018).Article 
CAS 

Google Scholar 
Dorador, J., Rodríguez-Tovar, F. J., IODP Expedition 339 Scientists. Digital image treatment applied to ichnological analysis of marine core sediments. Facies 60, 39–44 (2014).Article 

Google Scholar 
Dorador, J. & Rodríguez-Tovar, F. J. High-resolution image treatment in ichnological core analysis: Initial steps, advances and prospects. Earth-Sci. Rev. 177, 226–237 (2018).ADS 
Article 

Google Scholar 
Taylor, A. M. & Goldring, R. Description and analysis of bioturbation and ichnofabric. J. Geol. Soc. 150, 141–148 (1993).ADS 
Article 

Google Scholar 
Cao, Y. M., Curran, A. H. & Glumac, B. Testing the use of photoshop and imageJ for evaluating ichnofabrics. 2015 GSA Annual Meeting in Baltimore, Maryland, USA, Paper No. 128-11 (The Geol. Soc. of Am., 2015). More

DataWe used temperature data, rainfall data, and data on the incidence of malaria collected from 1901 to 2015 for 43 African countries to construct networks to determine the relationships between transmission of malaria and climate change elements, especially temperature and rainfall. Data resolution is given by the latitude and longitude of the capital city for every country in Africa. Temperature and rainfall data are provided in terms of monthly averages in the country wise. The nodes in the network represent the country, and the edges in the network represent the relationship between countries. We collected malaria data from Harvard Dataverse35 and the world malaria report from the WHO31. Data for temperature and rainfall were obtained from the Climate Change Knowledge Portal of the World Bank Group36.Network generation and analysisThe networks were constructed by using the threshold method where the network depends on the mean, standard deviation, and the real number ((n)) used to control the features of the network. Therefore, data for temperature, rainfall, and the incidence of malaria were divided into six groups mostly comprising ranges of 20 years (1900–1920, 1921–1940, 1941–1960, 1961–1980, 1981–2000) as well as the period from 2001 to 2015. The missing data in Malaria incidence data are filled by the average amount of malaria incidence collected per year.In Table S1, a malaria report from the World Health Organization shows that the rate of death is directly proportional to the incidence of malaria35. The death toll in Africa from malaria is about 98% of world deaths from malaria. Such deaths in African regions decrease thanks to efforts the WHO, governments, and the private sector have been conducting to prevent them. Weather and climate are among the factors that drive increases in malaria infections in different areas.We consider networks based on the threshold method (see the “Methods and Materials” section below). First, we fill the missing malaria incidence data, and we calculate normalized Pearson correlation coefficients of three-time series between two countries. Then, we obtain a correlation matrix for the countries. We estimate the average value of the correlation coefficients from the time intervals 1901–1920, 1921–1940, 1941–1960, 1961–1980, 1981–2000, and 2001–2015 for three time series: temperature, rainfall, and incidence of malaria. We summarize the averages and standard deviations of the correlation coefficients, as shown in Table S2. The mean values from the correlation in temperature are high, compared to those for rainfall and the incidence of malaria. The standard deviations in temperature and rainfall are large, but the standard deviation for the incidence of malaria is small.We chose an ad hoc threshold value of the correlation coefficients to generate sparse networks. The characteristic values for (n) of the threshold are given in Table S3. We consider three types of thresholds in order to observe changes in the networks according to the threshold.Let us define the normalized variance of each time series. We considered time series (T_{i} left( t right)), (M_{i} left( t right)), and (R_{i} left( t right)) in country (i) for temperature, the incidence of malaria, and rainfall, respectively. We define normalized variance as$$r_{ij} = frac{{x_{i} left( t right)x_{j} left( t right) – x_{i} left( t right)x_{j} left( t right)}}{{sigma_{i} sigma_{j} }}$$
(1)
where (x_{i} left( t right)) = (T_{i} left( t right)), (M_{i} left( t right)), (R_{i} left( t right)). We obtained a Pearson correlation matrix for each time series as follows:$$R_{S} = left[ {begin{array}{*{20}c} {r_{11} } & cdots & {r_{1N} } \ vdots & {r_{ij} } & vdots \ {r_{N1} } & cdots & {r_{NN} } \ end{array} } right]$$
(2)
where (S = T, M, R).We calculated the average value, (overline{r }), and the standard deviation, (sigma), for the correlation coefficients of the matrix. We applied the threshold method to generate a sparse network from the correlation matrix. Two countries are connected in the correlation network if and only if the value of the correlation coefficient is greater than, or equal to, the threshold value:$$r_{{ij}} = left{ {begin{array}{*{20}c} 1 & {{text{if}};r_{{ij}} ge bar{r}{text{ + n}}sigma } \ 0 & {{text{otherwise}}} \ end{array} } right.$$
(3)
where (r_{ij}) is the correlation coefficient between two countries, and (n) is an element of real numbers ((n in {mathbb{R}})). The value of (n) determines whether the network is sparsely or densely connected.We use Python programming language, packages, numpy for mathematical functions and random number generator, pandas for data analysis and manipulations, networkx for creation, manipulation, and studying the structure of the complex network, matplotlib for visualization and plotting graph and base map for map projection and visualization of geographic information. More

Garber, P. A. Foraging strategies among living primates. Annu. Rev. Anthropol. 16, 339–364 (1987).Article 

Google Scholar 
Stephens, D. W. & Krebs, J. K. Foraging Theory (Princeton University Press, 1987).Book 

Google Scholar 
Felton, A. M. et al. Nutritional ecology of Ateles chamek in lowland Bolivia: How macronutrient balancing influences food choices. Int. J. Primatol. 30, 675–696 (2009).Article 

Google Scholar 
Marshall, A. J. & Wrangham, R. W. Evolutionary consequences of fallback foods. Int. J. Primatol. 28, 1219–1235 (2007).Article 

Google Scholar 
Rothman, J. M., Raubenheimer, D., Bryer, M. A. H., Takahashi, M. & Gilbert, C. C. Nutritional contributions of insects to primate diets: Implications for primate evolution. J. Hum. Evol. 71, 59–69 (2014).PubMed 
Article 

Google Scholar 
Felton, A. M. et al. Protein content of diets dictates the daily energy intake of a free-ranging primate. Behav. Ecol. 20, 685–690 (2009).Article 

Google Scholar 
Clare, E. L., Symondson, W. O. C. & Fenton, M. B. An inordinate fondness for beetles? Variation in seasonal dietary preferences of night-roosting big brown bats (Eptesicus fuscus). Mol. Ecol. 23, 3633–3647 (2014).PubMed 
Article 

Google Scholar 
Stevenson, P. R., Quinones, M. J. & Ahumada, J. A. Influence of fruit availability on ecological overlap among four neotropical primates at Tinigua National Park, Colombia. Biotropica 32, 533–544 (2000).Article 

Google Scholar 
Chapman, C. Patterns of foraging and range use by three species of neotropical primates. Primates 29, 177–194 (1988).Article 

Google Scholar 
Felton, A. M., Felton, A., Lindenmayer, D. B. & Foley, W. J. Nutritional goals of wild primates. Funct. Ecol. 23, 70–78 (2009).Article 

Google Scholar 
Kay, R. On the use of anatomical features to infer foraging behavior in extinct primates. In Adaptations for Foraging in Nonhuman Primates (eds Rodman, P. & Cant, J.) 21–53 (Columbia University Press, 1984).Chapter 

Google Scholar 
Bravo, S. P. Seed dispersal and ingestion of insect-infested seeds by black howler monkeys in flooded forests of the Parana River, Argentina: Insect-infested seed ingestion and dispersal. Biotropica 40, 471–476 (2008).Article 

Google Scholar 
Deluycker, A. M. Insect prey foraging strategies in Callicebus oenanthe in Northern Peru: Insect foraging in Callicebus oenanthe. Am. J. Primatol. 74, 450–461 (2012).PubMed 
Article 

Google Scholar 
Link, A. Insect-eating by spider monkeys. Neotropical Primates 11, 104–107 (2003).ADS 

Google Scholar 
MacKinnon, K. C. Food choice by juvenile capuchin monkeys (Cebus capucinus) in a tropical dry forest. In New Perspectives in the Study of Mesoamerican Primates (eds Estrada, A. et al.) 349–365 (Kluwer Academic Publishers, 2006). https://doi.org/10.1007/0-387-25872-8_17.Chapter 

Google Scholar 
Fonseca, M. L., Cruz, D. M., Acosta Rojas, D. C., Páez Crespo, J. & Stevenson, P. R. Influence of arthropod and fruit abundance on the dietary composition of highland Colombian woolly monkeys (Lagothrix lagotricha lugens). Folia Primatol. (Basel) 90, 240–257 (2019).Article 

Google Scholar 
Vargas, S. A. et al. Population density and ecological traits of highland woolly monkeys at Cueva de los Guacharos National Park, Colombia. In High Altitude Primates (eds Grow, N. B. et al.) 85–102 (Springer, 2014). https://doi.org/10.1007/978-1-4614-8175-1_5.Chapter 

Google Scholar 
Bryer, M. A. H., Chapman, C. A., Raubenheimer, D., Lambert, J. E. & Rothman, J. M. Macronutrient and energy contributions of insects to the diet of a frugivorous monkey (Cercopithecus ascanius). Int. J. Primatol. 36, 839–854 (2015).Article 

Google Scholar 
Gómez-Posada, C., Rey-Goyeneche, J. & Tenorio, E. A. Ranging responses to fruit and arthropod availability by a tufted capuchin group (Sapajus apella) in the Colombian Amazon. In Movement Ecology of Neotropical Forest Mammals (eds Reyna-Hurtado, R. & Chapman, C. A.) 195–215 (Springer International Publishing, 2019). https://doi.org/10.1007/978-3-030-03463-4_12.Chapter 

Google Scholar 
Mallott, E. K., Garber, P. A. & Malhi, R. S. Integrating feeding behavior, ecological data, and DNA barcoding to identify developmental differences in invertebrate foraging strategies in wild white-faced capuchins (Cebus capucinus): Mallott et al. Am. J. Phys. Anthropol. 162, 241–254 (2017).PubMed 
Article 

Google Scholar 
Defler, T. R. & Defler, S. B. Diet of a group of Lagothrix lagothricha lagothricha in southeastern Colombia. Int. J. Primatol. 17, 161–190 (1996).Article 

Google Scholar 
Di Fiore, A. Diet and feeding ecology of woolly monkeys in a western Amazonian rain forest. Int. J. Primatol. 25, 767–801 (2004).Article 

Google Scholar 
Stevenson, P. R., Quinones, M. J. & Ahumada, J. A. Ecological strategies of woolly monkeys (Lagothrix lagotricha) at Tinigua National Park, Colombia. Am. J. Primatol. 32, 123–140 (1994).PubMed 
Article 

Google Scholar 
Izawa, K. Foods and feeding behavior of monkeys in the upper Amazon basin. Primates 16, 295–316 (1975).Article 

Google Scholar 
Peres, C. A. Diet and feeding ecology of gray woolly monkeys (Lagothrix lagotricha cana) in central Amazonia: Comparisons with other atelines. Int. J. Primatol. 15, 333–372 (1994).Article 

Google Scholar 
Stevenson, P. R. Activity and ranging patterns of Colombian woolly monkeys in north-western Amazonia. Primates 47, 239–247 (2006).PubMed 
Article 

Google Scholar 
Milton, K. & Nessimian, J. L. Evidence for insectivory in two primate species (Callicebus torquatus lugens and Lagothrix lagothricha lagothricha) from northwestern Amazonia. Am. J. Primatol. 6, 367–371 (1984).PubMed 
Article 

Google Scholar 
Soini, P. A synecological study of a primate community in the Pacaya-Samiria National Reservee, Peru. Primate Conserv. 7, 63–71 (1986).
Google Scholar 
Pickett, S. B., Bergey, C. M. & Di Fiore, A. A metagenomic study of primate insect diet diversity: A metagenomic study of primate diet. Am. J. Primatol. 74, 622–631 (2012).CAS 
PubMed 
Article 

Google Scholar 
Estupiñan, L. & Muñoz, D. Estudio ecológico comparativo de la artropofauna presente en los receptáculos axilares de dos bromeliáceas epífitas en diferentes bosques andinos. In Estudios ecológicos del páramos y del bosque altoandino Cordillera Oriental de Colombia (eds Mora, L. & Sturm, H.) 679–696 (Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 1995).
Google Scholar 
Solé, R. V. & Montoya, M. Complexity and fragility in ecological networks. Proc. R. Soc. Lond. B Biol. Sci. 268, 2039–2045 (2001).Article 

Google Scholar 
Symondson, W. O. C. Molecular identification of prey in predator diets. Mol. Ecol. 15, 3790–3798 (2002).
Google Scholar 
Gunst, N., Boinski, S. & Fragaszy, D. M. Development of skilled detection and extraction of embedded prey by wild brown capuchin monkeys (Cebus apella apella). J. Comp. Psychol. 124, 194–204 (2010).PubMed 
Article 

Google Scholar 
Panger, M. A. et al. Cross-site differences in foraging behavior of white-faced capuchins (Cebus capucinus). Am. J. Phys. Anthropol. 119, 52–66 (2002).PubMed 
Article 

Google Scholar 
Agostini, I. & Visalberghi, E. Social influences on the acquisition of sex-typical foraging patterns by juveniles in a group of wild tufted capuchin monkeys (Cebus nigritus). Am. J. Primatol. 65, 335–351 (2005).PubMed 
Article 

Google Scholar 
Barnes, M. A. & Turner, C. R. The ecology of environmental DNA and implications for conservation genetics. Conserv. Genet. 17, 1–17 (2016).CAS 
Article 

Google Scholar 
Creer, S. et al. The ecologist’s field guide to sequence-based identification of biodiversity. Methods Ecol. Evol. 7, 1008–1018 (2016).Article 

Google Scholar 
Clare, E. L., Fraser, E. E., Braid, H. E., Fenton, M. B. & Hebert, P. D. N. Species on the menu of a generalist predator, the eastern red bat (Lasiurus borealis): Using a molecular approach to detect arthropod prey. Mol. Ecol. 18, 2532–2542 (2009).PubMed 
Article 

Google Scholar 
Thuo, D. et al. Food from faeces: Evaluating the efficacy of scat DNA metabarcoding in dietary analyses. PLoS One 14, e0225805 (2019).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Siegenthaler, A., Wangensteen, O. S., Benvenuto, C., Campos, J. & Mariani, S. DNA metabarcoding unveils multiscale trophic variation in a widespread coastal opportunist. Mol. Ecol. 28, 232–249 (2019).CAS 
PubMed 
Article 

Google Scholar 
De Barba, M. et al. DNA metabarcoding multiplexing and validation of data accuracy for diet assessment: Application to omnivorous diet. Mol. Ecol. Resour. 14, 306–323 (2014).PubMed 
Article 
CAS 

Google Scholar 
Esnaola, A., Arrizabalaga-Escudero, A., González-Esteban, J., Elosegi, A. & Aihartza, J. Determining diet from faeces: Selection of metabarcoding primers for the insectivore Pyrenean desman (Galemys pyrenaicus). PLoS One 13, e0208986 (2018).PubMed 
PubMed Central 
Article 

Google Scholar 
Mallott, E. K. & Amato, K. R. The microbial reproductive ecology of white-faced capuchins (Cebus capucinus). Am. J. Primatol. 80, e22896 (2018).PubMed 
Article 

Google Scholar 
Wray, A. K. et al. Predator preferences shape the diets of arthropodivorous bats more than quantitative local prey abundance. Mol. Ecol. 30, 855–873 (2021).PubMed 
Article 

Google Scholar 
Quiroga-González, C. et al. Monitoring the variation in the gut microbiota of captive woolly monkeys related to changes in diet during a reintroduction process. Sci. Rep. 11, 6522 (2021).ADS 
PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
Prada, C. M. & Stevenson, P. R. Plant composition associated with environmental gradients in tropical montane forests (Cueva de Los Guacharos National Park, Huila, Colombia). Biotropica 48, 568–576 (2016).Article 

Google Scholar 
García-Toro, C., Link, A., Páez Crespo, J. & Stevenson, P. R. Home range and daily traveled distances of highland Colombian woolly monkeys (Lagothrix lagothricha lugens): Comparing spatial data from GPS collars and direct follows. In Movement Ecology of Neotropical Forest Mammals (eds Reyna-Hurtado, R. & Chapman, C. A.) 173–193 (Springer International Publishing, 2019). https://doi.org/10.1007/978-3-030-03463-4_3.Chapter 

Google Scholar 
Baulu, J. & Redmond, D. E. Some sampling considerations in the quantitation of monkey behavior under field and captive conditions. Primates 19, 391–399 (1978).Article 

Google Scholar 
Julliot, C. Seed dispersal by red howling monkeys (Alouatta seniculus) in the tropical rain forest of French Guiana. Int. J. Primatol. 17, 239–258 (1996).Article 

Google Scholar 
Hurlbert, S. H. Pseudoreplication and the design of ecological field experiments. Ecol. Monogr. 54, 187–211 (1984).Article 

Google Scholar 
Russo, L., Stehouwer, R., Heberling, J. M. & Shea, K. The composite insect trap: An innovative combination trap for biologically diverse sampling. PLoS One 6, e21079 (2011).ADS 
CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Ohmart, C. P., Stewart, L. G. & Thomas, J. R. Phytophagous insect communities in the canopies of three Eucalyptus forest types in south-eastern Australia. Austral Ecol. 8, 395–403 (1983).Article 

Google Scholar 
Erwin, T. L. Tropical forests: Their richness in Coleoptera and other arthropod species. Coleopt. Bull. 36, 74–75 (1982).
Google Scholar 
Schowalter, T. D., Webb, J. W. & Crossley, D. A. Communtiy structure and nutrient content of canopy arthropods in clearcut and uncut forest ecosystems. Ecology 62, 1010–1019 (1981).Article 

Google Scholar 
Stevenson, P. R. Phenological patterns of woody vegetation at Tinigua Park, Colombia: Methodological comparisons with emphasis on fruit production. Caldasia 26, 125–150 (2004).
Google Scholar 
Vargas, I. & Stevenson, P. R. Patrones fenológicos en la Estación Biológica Mosiro Itajura-Caparú: Producción de frutos estimada a partir de transectos fenológicos y trampas de frutos. In Estación Biológica Mosiro Itajura-Caparú: Biodiversidad en el territorio Yagojé-Apaporis (eds Alarcón-Nieto, G. & Palacios, E.) 99–104 (Conservación Internacional Colombia, 2009).
Google Scholar 
Bautista, S. Patrones de productividad de frutos y dispersión de semillas en diferentes bosques de Colombia, y su relación con la biomasa de primates (2019).King, R. A., Read, D. S., Traugott, M. & Symondson, W. O. C. Invited Review: Molecular analysis of predation: A review of best practice for DNA-based approaches: Optimizing molecular analysis of predation. Mol. Ecol. 17, 947–963 (2008).CAS 
PubMed 
Article 

Google Scholar 
Mata, V. A. et al. How much is enough? Effects of technical and biological replication on metabarcoding dietary analysis. Mol. Ecol. 28, 165–175 (2019).CAS 
PubMed 
Article 

Google Scholar 
Zeale, M. R. K., Butlin, R. K., Barker, G. L. A., Lees, D. C. & Jones, G. Taxon-specific PCR for DNA barcoding arthropod prey in bat faeces: DNA barcoding. Mol. Ecol. Resour. 11, 236–244 (2011).CAS 
PubMed 
Article 

Google Scholar 
Jusino, M. A. et al. An improved method for utilizing high-throughput amplicon sequencing to determine the diets of insectivorous animals. Mol. Ecol. Resour. 19, 176–190 (2019).CAS 
PubMed 
Article 

Google Scholar 
Aldasoro, M. et al. Gaining ecological insight on dietary allocation among horseshoe bats through molecular primer combination. PLoS One 14, e0220081 (2019).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Taberlet, P., Bonin, A., Zinger, L. & Coissac, E. Environmental DNA: For Biodiversity Research and Monitoring (Oxford University Press, 2018).Book 

Google Scholar 
Callahan, B. J. et al. DADA2: High-resolution sample inference from Illumina amplicon data. Nat. Methods 13, 581–583 (2016).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Callahan, B. J., McMurdie, P. J. & Holmes, S. P. Exact sequence variants should replace operational taxonomic units in marker-gene data analysis. ISME J. 11, 2639–2643 (2017).PubMed 
PubMed Central 
Article 

Google Scholar 
Ratnasingham & Hebert. bold: The barcode of life data system (http://www.barcodinglife.org). Mol. Ecol. Notes 7, 355–364 (2007).Palmer, J. M., Jusino, M. A., Banik, M. T. & Lindner, D. L. Non-biological synthetic spike-in controls and the AMPtk software pipeline improve mycobiome data. PeerJ 6, e4925 (2018).PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
Phillips, C. A. & McGrew, W. C. Identifying species in chimpanzee (Pan troglodytes) feces: A methodological lost cause?. Int. J. Primatol. 34, 792–807 (2013).Article 

Google Scholar 
Liu, M., Clarke, L. J., Baker, S. C., Jordan, G. J. & Burridge, C. P. A practical guide to DNA metabarcoding for entomological ecologists. Ecol. Entomol. 45, 373–385 (2020).Article 

Google Scholar 
Porter, T. M. & Hajibabaei, M. Over 2.5 million COI sequences in GenBank and growing. PLoS One 13, e0200177 (2018).PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
Valentini, A., Pompanon, F. & Taberlet, P. DNA barcoding for ecologists. Trends Ecol. Evol. 24, 110–117 (2009).PubMed 
Article 

Google Scholar 
Deagle, B. E., Jarman, S. N., Coissac, E., Pompanon, F. & Taberlet, P. DNA metabarcoding and the cytochrome c oxidase subunit I marker: Not a perfect match. Biol. Lett. 10, 20140562 (2014).PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
Hajibabaei, M. et al. A minimalist barcode can identify a specimen whose DNA is degraded. Mol. Ecol. Notes 6, 959–964 (2006).CAS 
Article 

Google Scholar 
Hebert, P. D. N., Cywinska, A., Ball, S. L. & deWaard, J. R. Biological identifications through DNA barcodes. Proc. R. Soc. Lond. B Biol. Sci. 270, 313–321 (2003).CAS 
Article 

Google Scholar 
Piñol, J., Senar, M. A. & Symondson, W. O. C. The choice of universal primers and the characteristics of the species mixture determine when DNA metabarcoding can be quantitative. Mol. Ecol. 28, 407–419 (2019).PubMed 
Article 
CAS 

Google Scholar 
R Studio Team. R Studio: Integrated Development of R (Rstudio, PBC, 2020).Hijmans, R. & van Etten, J. raster: Geographic analysis and modeling with raster data (2012).Wickham, H. ggplot2: Elegant graphics for data analysis (2016).Di Fiore, A. & Rodman, P. S. Time allocation patterns of lowland woolly monkeys (Lagothrix lagotricha poeppigii) in a neotropical Terra Firma Forest. Int. J. Primatol. 22, 449–480 (2001).Article 

Google Scholar 
Dew, J. L. Foraging, food choice, and food processing by sympatric ripe-fruit specialists: Lagothrix lagotricha poeppigii and Ateles belzebuth belzebuth. Int. J. Primatol. 26, 1107–1135 (2005).Article 

Google Scholar 
Deblauwe, I. & Janssens, G. P. J. New insights in insect prey choice by chimpanzees and gorillas in Southeast Cameroon: The role of nutritional value. Am. J. Phys. Anthropol. 135, 42–55 (2008).PubMed 
Article 

Google Scholar 
de Carvalho Jr, O., Ferrari, S. F. & Strier, K. B. Diet of a muriqui group (Brachyteles arachnoides) in continuous primary forest. Primates 45, 201–204 (2004).Article 

Google Scholar 
Talebi, M., Bastos, A. & Lee, P. C. Diet of southern muriquis in continuous Brazilian Atlantic forest. Int. J. Primatol. 26, 1175–1187 (2005).Article 

Google Scholar 
Kowalzik, B. K., Pavelka, M. S. M., Kutz, S. J. & Behie, A. Parasites, primates, and ant-plants: Clues to the life cycle of Controrchis spp. in black howler monkeys (Alouatta pigra) in Southern Belize. J. Wildl. Dis. 46, 1330–1334 (2010).PubMed 
Article 

Google Scholar 
Tebbich, S., Taborsky, M., Fessl, B., Dvorak, M. & Winkler, H. Feeding behavior of four arboreal Darwin’s finches: Adaptations to spatial and seasonal variability. Condor 106, 95–105 (2004).Article 

Google Scholar 
Páez Crespo, J. Comportamiento y caracterización genética de churucos de montaña (Lagothrix lagothricha lugens): Inferencias en la filopatría de machos (Universidad de los Andes, 2016).
Google Scholar 
Blüthgen, N., Verhaagh, M., Goitía, W. & Blüthgen, N. Ant nests in tank bromeliads—An example of non-specific interaction. Insectes Soc. 47, 313–316 (2000).Article 

Google Scholar 
Huxley, C. Symbiosos between ants and epiphytes. Biol. Rev. 55, 321–340 (1980).Article 

Google Scholar 
Brehm, G., Pitkin, L. M., Hilt, N. & Fiedler, K. Montane Andean rain forests are a global diversity hotspot of geometrid moths: Hotspot of geometrid moths. J. Biogeogr. 32, 1621–1627 (2005).Article 

Google Scholar 
Myers, N., Mittermeier, R. A., Mittermeier, C. G., da Fonseca, G. A. B. & Kent, J. Biodiversity hotspots for conservation priorities. Nature 403, 853–858 (2000).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Campuzano, E. F., Ibarra-Núñez, G., Machkour-M’Rabet, S., Morón-Ríos, A. & Jiménez, M. L. Diversity and seasonal variation of ground and understory spiders from a tropical mountain cloud forest. Insect Sci. 27, 826–844 (2020).PubMed 
Article 

Google Scholar 
Miller, J. S. & Thiaucourt, P. Diversity of prominent moths (Lepidoptera: Noctuoidea: Notodontidae) in the cloud forests of northeastern Ecuador, with descriptions of 27 new species. Ann. Entomol. Soc. Am. 104, 1033–1077 (2011).Article 

Google Scholar 
Lambert, J. E. Primate digestion: Interactions among anatomy, physiology, and feeding ecology. Evol. Anthropol. 7, 8–20 (1998).Article 

Google Scholar 
Janiak, M. C. No evidence of copy number variation in acidic mammalian chitinase genes (CHIA) in new world and old world monkeys. Int. J. Primatol. 39, 269–284 (2018).Article 

Google Scholar 
Remis, M. J. & Dierenfeld, E. S. Digesta passage, digestibility and behavior in captive gorillas under two dietary regimens. Int. J. Primatol. 25, 825–845 (2004).Article 

Google Scholar 
Wolda, H. Seasonality of tropical insects. J. Anim. Ecol. 49, 277 (1980).Article 

Google Scholar 
Yanoviak, S. P., Walker, H. & Nadkarni, N. M. Arthropod assemblages in vegetative vs. humic portions of epiphyte mats in a neotropical cloud forest. Pedobiologia 48, 51–58 (2004).Article 

Google Scholar 
Augspurger, C. K. Seedling survival of tropical tree species: Interactions of dispersal distance, light-gaps, and pathogens. Ecology 65, 1705–1712 (1984).Article 

Google Scholar 
Richards, L. A. & Windsor, D. M. Seasonal variation of arthropod abundance in gaps and the understorey of a lowland moist forest in Panama. J. Trop. Ecol. 23, 169–176 (2007).Article 

Google Scholar 
Tercel, M. P. T. G., Symondson, W. O. C. & Cuff, J. P. The problem of omnivory: A synthesis on omnivory and DNA metabarcoding. Mol. Ecol. 30, 2199–2206 (2021).PubMed 
Article 

Google Scholar  More