The gut microbiota affects the social network of honeybees
Wilson, E. O. Sociobiology: The New Synthesis (Harvard Univ. Press, 1975).Diamond, J. M. & Ordunio, D. Guns, Germs, and Steel (Books on Tape, 1999).Couzin, I. D. et al. Self-organization and collective behavior in vertebrates. Adv. Study Behav. 32, 1–75 (2003).
Google Scholar
Keller, L. Adaptation and the genetics of social behaviour. Philos. Trans. R. Soc. Lond. B 364, 3209–3216 (2009).
Google Scholar
Kay, T., Keller, L. & Lehmann, L. The evolution of altruism and the serial rediscovery of the role of relatedness. Proc. Natl Acad. Sci. USA 117, 28894–28898 (2020).CAS
PubMed
PubMed Central
Google Scholar
Cryan, J. F. & Dinan, T. G. Mind-altering microorganisms: the impact of the gut microbiota on brain and behaviour. Nat. Rev. Neurosci. 13, 701–712 (2012).CAS
PubMed
Google Scholar
Johnson, K. V. A. & Foster, K. R. Why does the microbiome affect behaviour? Nat. Rev. Microbiol. 16, 647–655 (2018).CAS
PubMed
Google Scholar
Sherwin, E., Bordenstein, S. R., Quinn, J. L., Dinan, T. G. & Cryan, J. F. Microbiota and the social brain. Science 366, eaar2016 (2019).CAS
PubMed
Google Scholar
Desbonnet, L., Clarke, G., Shanahan, F., Dinan, T. G. & Cryan, J. F. Microbiota is essential for social development in the mouse. Mol. Psychiatry 19, 146–148 (2014).CAS
PubMed
Google Scholar
Sharon, G. et al. Human gut microbiota from autism spectrum disorder promote behavioral symptoms in mice. Cell 177, 1600–1618 (2019).CAS
PubMed
PubMed Central
Google Scholar
Zhang, M. et al. A quasi-paired cohort strategy reveals the impaired detoxifying function of microbes in the gut of autistic children. Sci. Adv. 6, eaba3760 (2020).CAS
PubMed
PubMed Central
Google Scholar
Wu, W.-L. et al. Microbiota regulate social behaviour via stress response neurons in the brain. Nature 595, 409–414 (2021).CAS
PubMed
PubMed Central
Google Scholar
Vuong, H. E., Yano, J. M., Fung, T. C. & Hsiao, E. Y. The microbiome and host behavior. Annu. Rev. Neurosci. 40, 21–49 (2017).CAS
PubMed
PubMed Central
Google Scholar
Douglas, A. E. Simple animal models for microbiome research. Nat. Rev. Microbiol. 17, 764–775 (2019).CAS
PubMed
Google Scholar
Schretter, C. E. Links between the gut microbiota, metabolism, and host behavior. Gut Microbes 11, 245–248 (2020).PubMed
Google Scholar
Liberti, J. & Engel, P. The gut microbiota–brain axis of insects. Curr. Opin. Insect Sci. 39, 6–13 (2020).PubMed
Google Scholar
O’Donnell, M. P., Fox, B. W., Chao, P.-H., Schroeder, F. C. & Sengupta, P. A neurotransmitter produced by gut bacteria modulates host sensory behaviour. Nature 583, 415–420 (2020).PubMed
PubMed Central
Google Scholar
Wilson, E. O. The Insect Societies (Harvard Univ. Press, 1971).Hölldobler, B. & Wilson, E. O. The Ants (Harvard Univ. Press, 1990).Teseo, S. et al. The scent of symbiosis: gut bacteria may affect social interactions in leaf-cutting ants. Anim. Behav. 150, 239–254 (2019).
Google Scholar
Vernier, C. L. et al. The gut microbiome defines social group membership in honey bee colonies. Sci. Adv. 6, eabd3431 (2020).CAS
PubMed
PubMed Central
Google Scholar
Li, L. et al. Gut microbiome drives individual memory variation in bumblebees. Nat. Commun. 12, 6588 (2021).CAS
PubMed
PubMed Central
Google Scholar
Choi, S. H. et al. Individual variations lead to universal and cross-species patterns of social behavior. Proc. Natl Acad. Sci. USA 117, 31754–31759 (2020).CAS
PubMed
PubMed Central
Google Scholar
Geffre, A. C. et al. Honey bee virus causes context-dependent changes in host social behavior. Proc. Natl Acad. Sci. USA 117, 10406–10413 (2020).CAS
PubMed
PubMed Central
Google Scholar
Kwong, W. K. & Moran, N. A. Gut microbial communities of social bees. Nat. Rev. Microbiol. 14, 374–384 (2016).CAS
PubMed
PubMed Central
Google Scholar
Bonilla-Rosso, G. & Engel, P. Functional roles and metabolic niches in the honey bee gut microbiota. Curr. Opin. Microbiol. 43, 69–76 (2018).CAS
PubMed
Google Scholar
Raymann, K. & Moran, N. A. The role of the gut microbiome in health and disease of adult honey bee workers. Curr. Opin. Insect Sci. 26, 97–104 (2018).PubMed
PubMed Central
Google Scholar
Zheng, H., Powell, J. E., Steele, M. I., Dietrich, C. & Moran, N. A. Honeybee gut microbiota promotes host weight gain via bacterial metabolism and hormonal signaling. Proc. Natl Acad. Sci. USA 114, 4775–4780 (2017).CAS
PubMed
PubMed Central
Google Scholar
Kešnerová, L. et al. Disentangling metabolic functions of bacteria in the honey bee gut. PLoS Biol. 15, e2003467 (2017).PubMed
PubMed Central
Google Scholar
Kešnerová, L. et al. Gut microbiota structure differs between honeybees in winter and summer. ISME J. 14, 801–814 (2020).PubMed
Google Scholar
Mersch, D. P., Crespi, A. & Keller, L. Tracking individuals shows spatial fidelity is a key regulator of ant social organization. Science 340, 1090–1093 (2013).CAS
PubMed
Google Scholar
Stroeymeyt, N. et al. Social network plasticity decreases disease transmission in a eusocial insect. Science 362, 941–945 (2018).CAS
PubMed
Google Scholar
Kao, A. B. & Couzin, I. D. Modular structure within groups causes information loss but can improve decision accuracy. Philos. Trans. R. Soc. Lond. B 374, 20180378 (2019).
Google Scholar
de Groot, A. P. Protein and amino acid requirements of the honeybee (Apis mellifica L.). Physiol. Comp. Oecol. 3, 197–285 (1953).
Google Scholar
Billard, J.-M. d-Amino acids in brain neurotransmission and synaptic plasticity. Amino Acids 43, 1851–1860 (2012).CAS
PubMed
Google Scholar
Marcaggi, P. & Attwell, D. Role of glial amino acid transporters in synaptic transmission and brain energetics. Glia 47, 217–225 (2004).PubMed
Google Scholar
Gage, S. L., Calle, S., Jacobson, N., Carroll, M. & DeGrandi-Hoffman, G. Pollen alters amino acid levels in the honey bee brain and this relationship changes with age and parasitic stress. Front. Neurosci. 14, 231 (2020).PubMed
PubMed Central
Google Scholar
Kawase, T. et al. Gut microbiota of mice putatively modifies amino acid metabolism in the host brain. Br. J. Nutr. 117, 775–783 (2017).CAS
PubMed
Google Scholar
Socha, E., Koba, M. & Koslinski, P. Amino acid profiling as a method of discovering biomarkers for diagnosis of neurodegenerative diseases. Amino Acids 51, 367–371 (2019).CAS
PubMed
Google Scholar
Tarlungeanu, D. C. et al. Impaired amino acid transport at the blood brain barrier is a cause of autism spectrum disorder. Cell 167, 1481–1494 (2016).CAS
PubMed
PubMed Central
Google Scholar
Maynard, T. M. & Manzini, M. C. Balancing act: maintaining amino acid levels in the autistic brain. Neuron 93, 476–479 (2017).CAS
PubMed
Google Scholar
Kurochkin, I. et al. Metabolome signature of autism in the human prefrontal cortex. Commun. Biol. 2, 234 (2019).PubMed
PubMed Central
Google Scholar
van der Velpen, V. et al. Systemic and central nervous system metabolic alterations in Alzheimer’s disease. Alzheimer’s Res. Ther. 11, 93 (2019).
Google Scholar
Aldana, B. I. et al. Glutamate–glutamine homeostasis is perturbed in neurons and astrocytes derived from patient iPSC models of frontotemporal dementia. Mol. Brain 13, 125 (2020).CAS
PubMed
PubMed Central
Google Scholar
Galizia, C. G., Eisenhardt, D. & Giurfa M. (eds) Honeybee Neurobiology and Behavior: A Tribute to Randolf Menzel (Springer Science & Business Media, 2011).Menzel, R. The honeybee as a model for understanding the basis of cognition. Nat. Rev. Neurosci. 13, 758–768 (2012).CAS
PubMed
Google Scholar
Ellegaard, K. M. & Engel, P. Genomic diversity landscape of the honey bee gut microbiota. Nat. Commun. 10, 446 (2019).CAS
PubMed
PubMed Central
Google Scholar
Bruno, F., Angilica, A., Cosco, F., Luchi, M. L. & Muzzupappa, M. Mixed prototyping environment with different video tracking techniques. In IMProVe 2011 International Conference on Innovative Methods in Product Design (eds Concheri, G. et al.) 105–113 (Libreria Internazionale Cortina Padova, 2011).R Core Team. R: A Language and Environment for Statistical Computing (R Foundation for Statistical Computing, 2020).Anderson, K. E., Rodrigues, P. A. P., Mott, B. M., Maes, P. & Corby-Harris, V. Ecological succession in the honey bee gut: shift in Lactobacillus strain dominance during early adult development. Microb. Ecol. 71, 1008–1019 (2016).CAS
PubMed
Google Scholar
Almasri, H., Liberti, J., Brunet, J. L., Engel, P. & Belzunces, L. P. Mild chronic exposure to pesticides alters physiological markers of honey bee health without perturbing the core gut microbiota. Sci. Rep. 12, 4281 (2022).CAS
PubMed
PubMed Central
Google Scholar
Pfaffl, M. W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29, e45 (2001).Gallup, J. M. in PCR Troubleshooting and Optimization: The Essential Guide (eds Kennedy, S. & Oswald, N.) 23–65 (Caister Academic Press, 2011).Martin, M. Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet. J. 17, 10–12 (2011).
Google Scholar
Callahan, B. J. et al. DADA2: high-resolution sample inference from Illumina amplicon data. Nat. Methods 13, 581–583 (2016).CAS
PubMed
PubMed Central
Google Scholar
McMurdie, P. J. & Holmes, S. phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data. PLoS ONE 8, e61217 (2013).CAS
PubMed
PubMed Central
Google Scholar
Davis, N. M., Proctor, D. M., Holmes, S. P., Relman, D. A. & Callahan, B. J. Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data. Microbiome 6, 1–14 (2018).
Google Scholar
Patassini, S. et al. Identification of elevated urea as a severe, ubiquitous metabolic defect in the brain of patients with Huntington’s disease. Biochem. Biophys. Res. Commun. 468, 161–166 (2015).CAS
PubMed
Google Scholar
Gonzalez-Riano, C., Garcia, A. & Barbas, C. Metabolomics studies in brain tissue: a review. J. Pharm. Biomed. Anal. 130, 141–168 (2016).CAS
PubMed
Google Scholar
Belle, J. E. L., Harris, N. G., Williams, S. R. & Bhakoo, K. K. A comparison of cell and tissue extraction techniques using high-resolution 1H-NMR spectroscopy. NMR Biomed. 15, 37–44 (2002).PubMed
Google Scholar
Wanichthanarak, K., Jeamsripong, S., Pornputtapong, N. & Khoomrung, S. Accounting for biological variation with linear mixed-effects modelling improves the quality of clinical metabolomics data. Comput. Struct. Biotechnol. J. 17, 611–618 (2019).PubMed
PubMed Central
Google Scholar
Bolger, A. M., Lohse, M. & Usadel, B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30, 2114–2120 (2014).CAS
PubMed
PubMed Central
Google Scholar
Dobin, A. et al. STAR: ultrafast universal RNA-seq aligner. Bioinformatics 29, 15–21 (2013).CAS
PubMed
Google Scholar
Wallberg, A. et al. A hybrid de novo genome assembly of the honeybee, Apis mellifera, with chromosome-length scaffolds. BMC Genomics 20, 275 (2019).PubMed
PubMed Central
Google Scholar
Li, H. et al. The Sequence Alignment/Map format and SAMtools. Bioinformatics 25, 2078–2079 (2009).PubMed
PubMed Central
Google Scholar
Robinson, M. D., McCarthy, D. J. & Smyth, G. K. edgeR: a bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics 26, 139–140 (2010).CAS
PubMed
Google Scholar
Ritchie, M. E. et al. limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Res. 43, e47 (2015).PubMed
PubMed Central
Google Scholar
Durinck, S., Spellman, P. T., Birney, E. & Huber, W. Mapping identifiers for the integration of genomic datasets with the R/Bioconductor package biomaRt. Nat. Protoc. 4, 1184–1191 (2009).CAS
PubMed
PubMed Central
Google Scholar
Falcon, S. & Gentleman, R. Using GOstats to test gene lists for GO term association. Bioinformatics 23, 257–258 (2007).CAS
PubMed
Google Scholar
Reijnders, M. J. & Waterhouse, R. M. Summary visualisations of gene ontology terms with GO-Figure! Front. Bioinform. 1, 638255 (2021).
Google Scholar More