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Tagging deployments and study subjectsTable 1 contains an overview of the fields in the metadata file (JWS_metadata.xlsx) providing extensive background details on each of the 79 tag deployments and 63 study subjects. The data in this file give essential contextual information needed to understand the methodological, environmental, and demographic factors surrounding the deployments, which are critical for further examination and hypothesis testing of the sensor data. These metadata fall into several specific categories, but are not limited to, (i) information on the deployed electronic devices (platform, model, Platform Transmitter Terminal identifications), (ii) sharks (unique identifying numbers, sex, length), (iii) capture event (date, location, duration, methodology, interaction type), and (iv) the reporting period (duration, linear surface travel distance).Table 1 Metadata descriptions of the sharks, tagging operations, and deployments for all tags included in the database.Full size tableFigure 1 illustrates a typical C. carcharias tagging operation. This involves a contracted commercial fishing vessel with purpose-built gears to capture sharks (Fig. 1a) and a research crew to handle animals, monitor health (Fig. 1b) and attach electronic tags (Fig. 1c). More details on the tagging program and its methodologies are provided elsewhere14,19,20. Figure. 2 provides summaries of the deployment schedule, geographic locations, devices, and capture operations. Of note, 39.7% (25/64) of all tagging operations involved collaborations with commercial fishery operators (Fig. 2f–h), whose engagement was temporarily impacted (Fig. 2a) during the scientific review process when the population was under consideration for US Endangered Species Act listing. Figure 3 displays the demographic focus on small juvenile C. carcharias, with modest deployment durations and travel distances.Fig. 1Depiction of a typical research operation for capturing and tagging juvenile White Sharks in the Southern California Bight. (a) Aquarium research vessel (RV Lucile) with crew approaching a contracted purse seine vessel containing a captured juvenile white shark. (b) Research crew on the RV Lucile leading the shark into a sling, where it is subsequently transferred to the vessel’s deck for tagging. (c) Successfully applied PAT and acoustic tags each positioned lateral of the dorsal fin, anchored via leaders, and affixed with titanium darts (yellow arrows). All images taken by Steve McNicholas (Great White Shark 3D) for the Monterey Bay Aquarium and used with permission.Full size imageFig. 2Metadata summaries of the field program that deployed biologging tags on juvenile white sharks in the southern California Current. (a) Deployment schedule for 72 electronic tags released on 64 White Sharks from 2001–2020 (b) Tagging activity peaked in the late summer months when the population is most locally abundant. Field operations decreased from 2011–2013 when the population was being considered for listing under the U.S. Endangered Species Act (ESA). (c) Deployments focused on opportunities in the Southern California Bight coastline and included deployments in the nursery area of Bahía Sebastian Vizcaíno, Mexico and releases after exhibition at the Monterey Bay Aquarium. (d) Researchers released a variety of pop-up archival transmitting (PAT, 58 sharks), acoustic (21 sharks), and smart position and temperature (SPOT, 20 sharks) tags. This manuscript only reports the geolocation, temperature and depth data from the PAT and SPOT platforms. (e) Half (35 of 64, 54.7%) of all sharks received multiple tags, primarily to compare their relative performance. (f) Most tags (38 of 64, 60.3%) were deployed during focused scientific research operations. (g) The remainder were joint operations resulting from opportunistic bycatch in commercial fisheries using various gears and (h) Targeting various species. “Jab” gear refers to research operations that uses pole extensions to apply tags to sharks without capturing and handling.Full size imageFig. 3Demographic and deployment summaries from the juvenile white shark tagging program. (a) Total body length (TL) histogram indicates that most individuals tagged were either neonates ( More

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01 April 2022

Tropical forests have big climate benefits beyond carbon storage

Study finds that trees cool the planet by one-third of a degree through biophysical mechanisms such as humidifying the air.

Freda Kreier

Freda Kreier

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Tropical forests create cloud cover that reflects sunlight and cools the air.Credit: Thomas Marent/Minden Pictures

Tropical forests have a crucial role in cooling Earth’s surface by extracting carbon dioxide from the air. But only two-thirds of their cooling power comes from their ability to suck in CO2 and store it, according to a study1. The other one-third comes from their ability to create clouds, humidify the air and release cooling chemicals.
How much can forests fight climate change?
This is a larger contribution than expected for these ‘biophysical effects’ says Bronson Griscom, a forest climate scientist at the non-profit environmental organization Conservation International, headquartered in Arlington, Virginia. “For a while now, we’ve assumed that carbon dioxide alone is telling us essentially all we need to know about forest–climate interactions,” he says. But this study confirms that tropical forests have other significant ways of plugging into the climate system, he says.The analysis, published in Frontiers in Forests and Global Change on 24 March1, could enable scientists to improve their climate models, while helping governments to devise better conservation and climate strategies.The findings underscore growing concerns about rampant deforestation across the tropics. Scientists warn that one-third of the world’s tropical forests have been mown down in the past few centuries, and another one-third has been degraded by logging and development. This, when combined with climate change, could transform vast swathes of forest into grasslands2.“This study gives us even more reasons why tropical deforestation is bad for the climate,” says Nancy Harris, forest-research director at the World Resources Institute in Washington DC.More than a carbon spongeForests are major players in the global carbon cycle because they soak up CO2 from the atmosphere as they grow. Tropical forests, in particular, store around one-quarter of all terrestrial carbon on the planet, making them “centrepieces for climate policy” in their home countries, Griscom says.
Tropical forests may be carbon sources, not sinks
“There’s clear evidence that the tropics are producing excellent climate benefits for the entire planet,” says Deborah Lawrence, an environmental scientist at the University of Virginia in Charlottesville and a co-author of the latest study. She and her colleagues analysed the cooling capacity of forests around the globe, in particular considering biophysical effects alongside carbon storage. Tropical forests, they found, can cool Earth by a whole 1 °C — and biophysical effects contribute significantly.Although scientists knew about these effects, they hadn’t understood to what extent the various factors counter global warming.Trees in the tropics provide shade, but they also act as giant humidifiers by pulling water from the ground and emitting it from their leaves, which helps to cool the surrounding area in a way similar to sweating, Griscom says.“If you go into a forest, it immediately is a considerably cooler environment,” he says.This transpiration, in turn, creates the right conditions for clouds, which like snow and ice in the Arctic, can reflect sunlight higher into the atmosphere and further cool the surroundings. Trees also release organic compounds — for example, pine-scented terpenes — that react with other chemicals in the atmosphere to sometimes create a net cooling effect.Locally coolTo quantify these effects, Lawrence and her colleagues compared how the various effects of forests around the world feed into the climate system, breaking down their contributions in bands of ten degrees of latitude. When they considered only the biophysical effects, the researchers found that the world’s forests collectively cool the surface of the planet by around 0.5 °C.
When will the Amazon hit a tipping point?
Tropical forests are responsible for most of that cooling. But this band of trees across Latin America, Central Africa and southeast Asia is under increasing pressure from climate change and deforestation. Both of these human-caused impacts can lead rainforests to dry out, says Christopher Boulton, a geographer at the University of Exeter, UK. Last month, he and his colleagues published a review2 of nearly 30 years’ worth of satellite images of the Amazon, the largest rainforest in the world. By measuring the biomass of the vegetation in the images, the team discovered that three-quarters of the Amazon is losing resilience — the ability to recover from an extreme weather event such as a drought.Threats to tropical rainforests are dangerous not only for the global climate, but also for communities that neighbour the forests, Lawrence says. She and her colleagues found that the cooling caused by biophysical effects was especially significant locally. Having a rainforest nearby can help to protect an area’s agriculture and cities from heatwaves, Lawrence says. “Every tenth of a degree matters in limiting extreme weather. And where you have forests, the extremes are minimized.”Governments across the tropics have struggled to conserve their forests despite more than two decades of global campaigns to halt deforestation, promote sustainable development and protect the climate. Lawrence says that her team’s findings make it clear that protecting forests is a matter of self-interest, and has immediate benefits for local communities.

doi: https://doi.org/10.1038/d41586-022-00934-6

ReferencesLawrence, D., Coe, M., Walker, W., Verchot, L. & Vandecar, K. Front. For. Glob. Change https://doi.org/10.3389/ffgc.2022.756115 (2022).Article 

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Boulton, C. A., Lenton, T. M. & Boers, N. Nature Clim. Change 12, 271–278 (2022).Article 

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Jobs

Postdoctoral Fellow in Electrochemical CO2 reduction

The University of British Columbia (UBC)
Kelowna, Canada

Staff Member in Project Coordination (for 19 h/week)

Jülich Research Centre (FZJ)
Erlangen-Nürnberg, Germany

Student assistant IT administration (m/f/d)

Alfred Wegener Institute – Helmholtz Centre for Polar and Marine Research (AWI)
Potsdam, Germany

Physicist (postdoctoral researcher) (all genders) in the field of Theoretical Astrophysics

Helmholtz Centre for Heavy Ion Research GmbH (GSI)
Darmstadt, Germany More

To explore how environmental gradients shape the distribution of cyanophages and picocyanobacteria, we conducted high-resolution surveys in surface waters along five oceanic transects on three cruises covering thousands of kilometres in the North Pacific Ocean in the spring or early summer of 2015, 2016 and 2017 (Fig. 1a–c). These cruises, two of which were out-and-back, passed through distinct regimes from warm, saline and nutrient-poor waters of the North Pacific Subtropical Gyre to cooler, less saline and nutrient-rich waters of higher latitudes influenced by the subpolar gyre (Fig. 1d–i)27. The shift between the two gyres was marked by abrupt changes in trophic indicators such as particulate carbon concentrations (Fig. 1g) and a chlorophyll front (defined as the 0.2 mg m−3 chlorophyll contour28; Fig. 1a–c). As such, the inter-gyre transition zone, defined by salinity and temperature thresholds29 (Fig. 1d), was distinct from both the subtropical and subpolar gyre ecosystems28.Fig. 1: Gradients in environmental conditions across the North Pacific gyres.a–c, Transects of three cruises overlaid on monthly averaged satellite-derived sea-surface chlorophyll in March 2015 (a), April 2016 (b) and June 2017 (c). d, Temperature–salinity diagram showing the boundaries of the subtropical and subpolar gyres (black dashed lines) based on the salinity thresholds reported by Roden29. e–i, Temperature (e), salinity (f) as well as the levels of particulate carbon (g), phosphate (h) and nitrate + nitrite (i) as a function of latitude. The coloured dashed lines show the position of the 0.2 mg m−3 chlorophyll contour. For environmental variables plotted against temperature, see Supplementary Fig. 3.Full size imageUnexpected Prochlorococcus declineProchlorococcus concentrations in the oligotrophic waters of the subtropical gyre were 1.5–3.0 × 105 cells ml−1, comprising an average of approximately 29% of the total bacteria (Extended Data Fig. 1) and numerically dominating the phytoplankton community in all three cruises (Extended Data Fig. 2). Prochlorococcus abundance remained high in the southern region of the transition zone in 2015 and 2016, decreasing precipitously to less than 2,000 cells ml−1 north of the chlorophyll front, generally constituting 80% of cyanophages measured, with the remainder consisting of T7-like clade A and TIM5-like cyanophages (Fig. 3 and Extended Data Fig. 4). Cyanophage abundances correlated positively with total picocyanobacteria in the subtropical gyre (Pearson’s coefficient of multiple correlation (r) = 0.54, P = 0.02, n = 26; Fig. 2d), suggesting that cyanophages were limited by the availability of susceptible hosts in this region and were not regulating picocyanobacterial populations. On average, less than 1% of the cyanobacterial populations were infected (Fig. 4), with higher infection rates by T4-like cyanophages than T7-like cyanophages (Extended Data Figs. 5 and 6). These instantaneous measurements of infection were used to estimate the daily rates of mortality39 (Methods and Supplementary Discussion), which suggests that 0.5–6% of picocyanobacterial populations were lysed by viruses each day (Extended Data Fig. 7). This implicates other factors, such as grazing45, as the major causes of cyanobacterial mortality in the North Pacific Subtropical Gyre.Fig. 3: Cyanophage community composition across the North Pacific gyres.a–c, Cyanophage abundance for the March 2015 (a), April 2016 (b) and June 2017 (c) transects. Insets: T7-like clade A and TIM5-like cyanophage abundances on an expanded scale (similar to the main images, the units for the vertical axes are ×105 viruses ml−1). The grey shaded regions show the position of the virus hotspot. See Extended Data Fig. 4 for the confidence intervals and out-and-back reproducibility and Supplementary Fig. 4 for cyanophage lineages plotted against latitude.Full size imageFig. 4: Viral infection patterns of picocyanobacteria in the North Pacific Ocean.a–f, Viral infection levels (black) of Prochlorococcus (a,c,e) and Synechococcus (b,d,f) plotted against temperature for the March 2015 (a,b), April 2016 (c,d) and June 2017 (e,f) transects. Insets: infection levels on an expanded scale. The solid lines show infection (red), Prochlorococcus (green) and Synechococcus (pink) averaged and plotted for every 0.5 °C. The dashed lines and shaded regions show the position of the chlorophyll front and the virus hotspot, respectively. For plots by latitude and the upper and lower bounds of infection, see Extended Data Figs. 5 and 6.Full size imageWithin the transition zone we observed a steep latitudinal increase in the abundance of cyanophages for every transect, which we define as a cyanophage hotspot (Fig. 2c and Extended Data Figs. 2 and 4). The cyanophage abundances in this hotspot were between three- and tenfold greater than in the subtropical gyre (Fig. 2c). Notably, cyanophages were approximately 25% more abundant (an increase of approximately 5 × 105 viruses ml−1) in the hotspot on the 2017 cruise relative to the other two cruises, reaching a maximum of 2 × 106 viruses ml−1. The hotspot peaked at temperatures of 15–16 °C on all transects, regardless of the geographical location, season or the exact pattern of the Prochlorococcus and Synechococcus distributions (Fig. 2c). Notably, the numbers of T7-like clade B cyanophages increased sharply in the transition zone to become the most abundant lineage, whereas T4-like cyanophages increased more modestly (Fig. 3 and Extended Data Fig. 4). The change in the cyanophage community structure was particularly pronounced in June 2017, when T7-like cyanophages were up to 2.3-fold more abundant than T4-like cyanophages (Fig. 3c). The switch in the relative abundance of T4-like and T7-like clade B cyanophages was diagnostic of the cyanophage hotspot compared with patterns in the subtropical and subpolar gyres.To begin assessing whether cyanophages negatively affected cyanobacterial populations in the hotspot, we tested the relationship between the abundance of cyanophages and total cyanobacteria. This showed a significant negative correlation between cyanophage and cyanobacterial abundances across all three cruises (Pearson’s r = −0.56, two-sided P = 0.0005, n = 34). This relationship was particularly distinct in 2017, when cyanobacteria were at their overall lowest abundances and cyanophages at their highest (Pearson’s r = −0.65, two-sided P = 0.004, n = 18). This suggests that viruses are one of the key regulators of picocyanobacteria in the region of the hotspot. However, no significant correlation was found across all regimes and all years (Pearson’s r = −0.008, two-sided P = 0.9, n = 87; Fig. 2d), indicating that factors other than viruses are likely to be more important in regulating the abundances of cyanobacteria in other regimes.Our single-cell infection measurements allowed us to directly evaluate active viral infection and its impact on picocyanobacteria in the transition zone. Viral infection spiked in this region each year with infection levels that were an average of two- to ninefold higher than those in the subtropical gyre (Fig. 4 and Extended Data Figs. 5,6 and 8). Infection peaked within the temperature range of 12–18 °C and was associated with a concomitant dip in Prochlorococcus abundances in all three cruises (Fig. 4 and Extended Data Fig. 5). These findings provide independent support for the strong negative correlation between cell and virus abundances (Fig. 2d) being the result of virus-induced mortality.Lineage-specific infection was also distinct in the transition zone relative to the subtropical gyre. Infection by T7-like clade B cyanophages generally increased to reach (2015 and 2016) or exceed (2017) those of T4-like cyanophages (Extended Data Figs. 5 and 6). In addition, the ratio of the abundances of T7-like clade B cyanophages to the number of cells they infected was 2.6-fold greater in the hotspot than the subtropics, whereas this ratio was similar in both regions for T4-like cyanophages. Together, these results indicate that, within the hotspot, the T4-like cyanophages displayed increased levels of infection, whereas the T7-like cyanophages displayed both increased levels of infection and produced more viruses per infection, suggesting that T7-like clade B cyanophages are better adapted to conditions in the transition zone (see below).Of the three cruises, the highest levels of viral infection were observed in June 2017, with up to 9.5% and 8.9% of Prochlorococcus and Synechococcus infected, respectively (Fig. 4e,f). This dramatic increase in infection mirrored the massive decline in Prochlorococcus abundances (Fig. 4e and Extended Data Fig. 5i). We estimate that viruses killed 10–30% of Prochlorococcus and Synechococcus cells daily at these high instantaneous levels of infection (Extended Data Fig. 6) based on the expected number of infection cycles cyanophages were able to complete at the light and temperature conditions in the transition zone (Methods and Supplementary Discussion). Given that Prochlorococcus is estimated to double every 2.8 ± 0.8 d at the low temperatures in this region12, we estimate that 21–51% of the population was infected and killed in the interval before cell division. Synechococcus is expected to have faster growth rates at these temperatures, doubling every 1.1 ± 0.2 d (refs. 12,46). Thus, we estimate that less of the Synechococcus population (9–31%) was killed before division.Under quasi-steady state conditions, abiotic controls on the growth rate of Prochlorococcus are balanced by mortality due to viral lysis, grazing and other mortality agents39,45,47. Based on the high levels of virus-mediated mortality, the parallel pattern between Prochlorococcus’ death and viral infection, and the negative correlation between cyanophage and picocyanobacterial abundances in the transition zone, we propose that enhanced viral infection in 2017 disrupted this balance, leading to the unexpected decline in Prochlorococcus populations. Grazing and other mortality agents not investigated here could also have contributed to additional mortality beyond the steady state, resulting in further losses of Prochlorococcus. In contrast to Prochlorococcus, Synechococcus maintained large populations despite high levels of infection (Fig. 4f), presumably due to their faster growth rates enabling them to maintain a positive net growth despite enhanced mortality. These findings suggest that virus-mediated mortality in 2017 was an important factor in limiting the geographic range of Prochlorococcus that resulted in a massive loss of habitat of approximately 550 km.Cyanophage abundances and infection levels dropped sharply in the higher-latitude waters north of the hotspot (Figs. 2c, 4 and Extended Data Figs. 1d,h and 2). The abundances of both T7-like clade B and T4-like cyanophages declined precipitously, yet T4-like cyanophages were the dominant cyanophage lineage (Fig. 3). T7-like clade A cyanophages generally increased locally at the northern border of the hotspot and became the dominant T7-like lineage in two samples between 38 and 39.2° N in 2017 (Fig. 3c and Extended Data Fig. 4). In contrast to all other cyanophages, the abundances of TIM5-like cyanophages increased in waters north of the hotspot (Fig. 3 and Extended Data Fig. 4d,i,m) but remained a minor component of the cyanophage community. No relationship was found between cyanophage and cyanobacterial abundances (Fig. 2d), and less than 1.5% of picocyanobacteria were infected by all cyanophage lineages in these waters (Fig. 4).The cyanophage hotspot in the transition zone is a ridge of high virus activity that separates the subtropical and subpolar gyres. The reproducibility of our observations, which were separated by days to weeks within each cruise (2016 and 2017) and by years among the three cruises (Extended Data Fig. 4), indicates that this virus hotspot is a recurrent feature at the boundary of these two major gyres in the North Pacific Ocean. This suggests that the hotspot forms due to the distinctive environment of the inter-gyre transition zone creating conditions that enhance infection of picocyanobacteria and proliferation of cyanophages. Prochlorococcus in the transition zone may be prone to stress due to being close to the limits of their temperature growth range5,6, which has the potential to increase susceptibility to viral infection. Alternatively, there may be temperature-dependent trade-offs between virus decay and production that lead to replication optima within a narrow temperature range48. Cyanophage infectivity has been observed to decay more slowly at colder temperatures49, which may allow for the accumulation of infective viruses, leading to increased infection. In addition, cyanophage infections may be more productive due to enhanced nutrient supply in the transition zone27 (Fig. 1h,i) relative to the subtropics, given that the cyanophages replicate in hosts with presumably greater intracellular nutrient quota and obtain more extracellular nutrients, both of which may increase progeny production9,10. The environmental factors influencing the production and removal of viruses probably vary in intensity at different times, leading to variability in cyanophage abundance and infection levels. Thus, the putative cyanophage replication optimum in the hotspot may reflect the combined effects of temperature and nutrient conditions that are intrinsically linked to the oceanographic forces that shape the transition zone itself.Changes in the cyanophage community structure over environmental gradients are likely to reflect differences in host range, infection properties and genomic potential to remodel host metabolism9. Our data, together with previous measurements in the North Pacific Subtropical Gyre38,39, indicate that the T4-like cyanophages are the lineage best adapted to the low-nutrient waters of the subtropics (Fig. 2d–f). As these waters are inhabited by hundreds of genomically diverse subpopulations of Prochlorococcus50, the broad host range of many T4-like cyanophages18,19,22,51 may be advantageous for finding a suitable host. T4-like cyanophages also have a large and diverse repertoire of host-derived genes21,51—such as nutrient acquisition, photosynthesis and carbon-metabolism genes—that augment host metabolism52 and may increase fitness in nutrient-poor conditions in the subtropics51. In contrast, T7-like clade A and B cyanophages seem to be better adapted to conditions in the transition zone (Fig. 3). T7-like cyanophages have narrow host ranges19,22,40, with smaller genomes and fewer genes to manipulate the host metabolism23, which may allow them to replicate and produce more progeny in regions with elevated nutrient concentrations relative to subtropical conditions. The maximal abundances of TIM5-like cyanophages were found in the most productive waters at the northern end of the transects where the cyanobacterial abundances were lowest and Synechococcus was the dominant picocyanobacterium. This may be partially due to the narrow host range of TIM5-like cyanophages and their specificity for Synechococcus40,44. Our findings of reproducible lineage-specific responses to changing ocean regimes indicate that cyanophage lineages occupy distinct ecological niches.Temperature and nutrient changes occurring in the transition zone are expected to result in shifts in picocyanobacterial diversity at the sub-genus level (Supplementary Discussion), which we speculate may affect community susceptibility to viral infection. One mechanism for this may be that the picocyanobacteria that thrive in the transition zone are intrinsically more susceptible to viral infection. Another scenario may be related to trade-offs associated with the evolution of resistance to viral infection. The horizontal advection of nutrient-rich waters to the transition zone28 may select for rapidly growing cells adapted for efficient resource utilization. Viral resistance in picocyanobacteria often incurs the cost of reduced growth rates53,54. Thus, competition for nutrients in this region may favour cells with faster growth rates but increased susceptibility to viral infection. Thus, it is probable that the cyanophage distributions do not always follow the cyanobacterial patterns (Extended Data Fig. 2) because of complex interactions between lineage-specific cyanophage traits, host community structure and environmental variables, which may vary seasonally or annually as a result of interannual variability in environmental conditions (see below).Despite consistent features in cyanophage distributions across the North Pacific Ocean, cyanophage infection was higher (Fig. 4 and Extended Data Fig. 7), whereas Prochlorococcus abundances were consistently lower (Fig. 2a), across the June 2017 transects relative to the March 2015 and April 2016 transects. Seasonality and/or climate variability could explain this interannual variability, although the data currently available to assess this are sparse. Viral infection of picocyanobacteria in the subtropical gyre increased from early spring to summer, suggesting a potential seasonal pattern that may extend across the transect (Extended Data Fig. 9a). In addition, the June 2017 transect occurred during a neutral-to-negative El Niño phase with lower sea-surface temperatures relative to the 2015 and 2016 transects, which were in years of a record marine heatwave, followed by a strong El Niño55 (Extended Data Fig. 9b). In 2015 and 2016, the Prochlorococcus abundances were found to be higher than usual in the North Pacific Ocean in this (Fig. 2a) and other studies56,57. Irrespective of the underlying drivers for the observed interannual variability, we speculate that an ecosystem tipping point was reached in the hotspot under the prevailing conditions in June 2017, aided by the higher cyanophage abundances yet smaller Prochlorococcus population sizes. In this scenario, picocyanobacterial populations were subjected to high infection levels that resulted in an accumulation of cyanophages, initiating a stronger than usual positive-feedback loop between infection and virus production, and precipitating the unexpected Prochlorococcus decline. Continued observations in the North Pacific Ocean are needed to evaluate the potential link between seasonality and/or large-scale climate forcing as ultimate drivers affecting virus–host interactions.Predicting basin-scale virus dynamicsMeasurements of cyanobacterial and cyanophage abundances rely on discrete sample collection from shipboard oceanographic expeditions, which limits the geographical and seasonal extent of available data. Therefore, we developed a multiple regression model based on high-resolution satellite data of temperature and chlorophyll to predict cyanophage abundances, a key proxy of cyanobacterial infection (Pearson’s r = 0.61, two-sided P = 1.7 × 10−8, degrees of freedom = 68, n = 70). We used the model to estimate the geographical extent of the virus hotspot. The model accurately predicted the location of the hotspot and cyanophage abundances along a fourth transect in April 2019 (Supplementary Table 1), with the majority of observations falling within the 95% confidence intervals of the model predictions (Fig. 5a–c). Application of the model to the larger region predicted that the virus hotspot formed a boundary extending across the North Pacific Ocean, with lower cyanophage abundances on both sides (Fig. 5d,e and Supplementary Fig. 1). This boundary had the hallmarks of the hotspot with a core that was dominated by T7-like cyanophages and the flanking gyre regions dominated by T4-like cyanophages. Thus, this feature may be more appropriately termed a ‘hot-zone’ due to its substantial projected aerial extent. Assuming the infection levels observed in the hotspot in June 2017 were similar throughout the hot-zone, the potential habitat loss for Prochlorococcus would be about 3.2 × 106 km2, approximately half of the cumulative area loss of the Amazonian rainforest to date58.Fig. 5: Prediction of cyanophage abundances.a–c, Model-based predictions of cyanophage abundances corresponding to the empirically measured total (a), T4-like (b) and T7-like clade B (c) cyanophage abundances along a transect in the North Pacific in April 2019. The shaded regions show the 95% confidence interval for the model predictions. d,e, Predicted total cyanophages (d) and the ratio of T4-like/T7-like clade B cyanophages (e) in June 2017 in the North Pacific Ocean. The black lines indicate the cruise track. The grey areas represent regions with no values due to cloud cover or that were beyond the limits of the predictive model. The hotspot peak corresponds to yellow regions in d and red regions in e.Full size imageVirus hotspot biogeochemistryWith the ability to predict biogeographic patterns of cyanophages, we evaluated the potential biogeochemical implications of virus-mediated picocyanobacterial lysis and release of organic material in sustaining the bacterial community6,7,8,9. The aerial extent of the hot-zone (approximately 4 × 106 km2) is only 14% of the size of the subtropical gyre (2.9 × 107 km2), and yet the total virus-mediated organic matter released from picocyanobacteria in the hot-zone in June 2017 was estimated to be on par with that for the entire North Pacific Subtropical Gyre (Methods and Supplementary Discussion). We estimate that viral lysate released from picocyanobacteria in the subtropical gyre could sustain 4.4 ± 0.8% of the calculated bacterial carbon demand there (Extended Data Fig. 10). In contrast, viral lysate released in the transition zone could sustain an average of 21 ± 12% of the bacterial carbon demand, reaching 33% in some regions (Extended Data Fig. 10), assuming that the bacterial assimilation and growth efficiencies were similar between the subtropical gyre and the hotspot. Thus, local generation of cyanobacterial viral lysate in the transition zone is likely to be an important source of carbon for the heterotrophic bacterial community that can rapidly utilize large molecular weight dissolved organic matter59 and may have contributed to the increase in their abundances south of the chlorophyll front in 2017 (Extended Data Fig. 1a,e). More

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