Philippa Kaur

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This picture was taken at night in the coastal community of Dakenggu in Yilan County, which is just southeast of Taipei in Taiwan. I’m on the left, working with two other researchers to measure the body size of a red-clawed crab (Chiromantes haematocheir).An old man from the local community told me that years ago, during the breeding season, you could barely cross the road because of all the crabs. He said nobody knows where they all went. They’re an important memory for the local people, and part of the culture here.Habitat loss — especially resulting from the widespread use of concrete — seems to be driving the decline. I’m working with local people to create rocky microhabitats and artificial wetlands for the red-clawed crabs to live in. They’re important scavengers — eating dead animals and other organic matter, breaking it down and playing a key part in the nutrient cycle.Small organisms need our help — they can’t stand up for themselves. But in Taiwan, a lot of people think a coastal villa is more important than a few crabs. Corporations want to build luxury developments in our national parks, and authorities often approve them. I’ve seen so many intact habitats destroyed or covered in concrete.Crabs caught my interest because they were frequent visitors to my dormitory. National Sun Yat-sen University in Kaohsiung sits in a coastal buffer zone between a mountain and the ocean, and land hermit crabs (Coenobita cavipes) have to scurry through it on their way to breed.After watching habitat after habitat destroyed by overdevelopment, I’ve realized that just doing the science is not enough. It doesn’t matter how many papers you publish: you need to connect with people through education and communication. That’s why I decided to do my PhD in social science. And it’s why I believe conservation will be my life’s work.

Nature 603, 962 (2022)
doi: https://doi.org/10.1038/d41586-022-00810-3

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Poirel, L. et al. Tn125-related acquisition of blaNDM-like genes in Acinetobacter baumannii. Antimicrob. Agents Chemother. 56, 1087–1089 (2012).CAS 
PubMed 
PubMed Central 

Google Scholar 
Wang, R. et al. The global distribution and spread of the mobilized colistin resistance gene mcr-1. Nat. Commun. 9, 1179 (2018).PubMed 
PubMed Central 

Google Scholar 
Clark, N. C., Weigel, L. M., Patel, J. B. & Tenover, F. C. Comparison of Tn1546-like elements in vancomycin-resistant Staphylococcus aureus isolates from Michigan and Pennsylvania. Antimicrob. Agents Chemother. 49, 470–472 (2005).CAS 
PubMed 
PubMed Central 

Google Scholar 
Partridge, S. R., Kwong, S. M., Firth, N. & Jensen, S. O. Mobile genetic elements associated with antimicrobial resistance. Clin. Microbiol. Rev. 31, e00088-17 (2018).PubMed 
PubMed Central 

Google Scholar 
Stokes, H. W. & Gillings, M. R. Gene flow, mobile genetic elements and the recruitment of antibiotic resistance genes into Gram-negative pathogens. FEMS Microbiol. Rev. 35, 790–819 (2011).CAS 

Google Scholar 
Ghaly, T. M. & Gillings, M. R. Mobile DNAs as ecologically and evolutionarily independent units of life. Trends Microbiol. 26, 904–912 (2018).CAS 

Google Scholar 
Modi, S. R., Lee, H. H., Spina, C. S. & Collins, J. J. Antibiotic treatment expands the resistance reservoir and ecological network of the phage metagenome. Nature 499, 219–222 (2013).CAS 
PubMed 
PubMed Central 

Google Scholar 
Brown-Jaque, M., Calero-Cáceres, W. & Muniesa, M. Transfer of antibiotic-resistance genes via phage-related mobile elements. Plasmid https://doi.org/10.1016/j.plasmid.2015.01.001 (2015).Frantzeskakis, L. et al. Signatures of host specialization and a recent transposable element burst in the dynamic one-speed genome of the fungal barley powdery mildew pathogen. BMC Genomics 19, 381 (2018).Scott, K. P. The role of conjugative transposons in spreading antibiotic resistance between bacteria that inhabit the gastrointestinal tract. Cell. Mol. Life Sci. 59, 2071–2082 (2002).CAS 
PubMed 
PubMed Central 

Google Scholar 
Pezzella, C., Ricci, A., DiGiannatale, E., Luzzi, I. & Carattoli, A. Tetracycline and streptomycin resistance genes, transposons, and plasmids in Salmonella enterica isolates from animals in Italy. Antimicrob. Agents Chemother. 48, 903–908 (2004).CAS 
PubMed 
PubMed Central 

Google Scholar 
Bengtsson-Palme, J., Boulund, F., Fick, J., Kristiansson, E. & Larsson, D. G. Shotgun metagenomics reveals a wide array of antibiotic resistance genes and mobile elements in a polluted lake in India. Front. Microbiol. 5, 648 (2014).PubMed 
PubMed Central 

Google Scholar 
Imchen, M. & Kumavath, R. Shotgun metagenomics reveals a heterogeneous prokaryotic community and a wide array of antibiotic resistance genes in mangrove sediment. FEMS Microbiol. Ecol. 96, fiaa173 (2020).CAS 

Google Scholar 
Zhang, T., Zhang, X.-X. & Ye, L. Plasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge. PLoS ONE 6, e26041 (2011).CAS 
PubMed 
PubMed Central 

Google Scholar 
Hu, H. et al. Novel plasmid and its variant harboring both a blaNDM-1 gene and type IV secretion system in clinical isolates of Acinetobacter lwoffii. Antimicrob. Agents Chemother. 56, 1698–1702 (2012).CAS 
PubMed 
PubMed Central 

Google Scholar 
Smet, A. et al. Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences. PLoS ONE 5, e11202 (2010).PubMed 
PubMed Central 

Google Scholar 
Revilla, C. et al. Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids. Antimicrob. Agents Chemother. 52, 1472–1480 (2008).CAS 
PubMed 
PubMed Central 

Google Scholar 
Poirel, L., Dortet, L., Bernabeu, S. & Nordmann, P. Genetic features of blaNDM-1-positive Enterobacteriaceae. Antimicrob. Agents Chemother. 55, 5403–5407 (2011).CAS 
PubMed 
PubMed Central 

Google Scholar 
Toleman, M. A., Spencer, J., Jones, L. & Walsh, T. R. blaNDM-1 is a chimera likely constructed in Acinetobacter baumannii. Antimicrob. Agents Chemother. 56, 2773–2776 (2012).CAS 
PubMed 
PubMed Central 

Google Scholar 
Bonnin, R. A., Poirel, L. & Nordmann, P. New Delhi metallo-β-lactamase-producing Acinetobacter baumannii: a novel paradigm for spreading antibiotic resistance genes. Future Microbiol. 9, 33–41 (2014).CAS 

Google Scholar 
Waterman, P. E. et al. Bacterial peritonitis due to Acinetobacter baumannii sequence type 25 with plasmid-borne New Delhi metallo-β-lactamase in Honduras. Antimicrob. Agents Chemother. 57, 4584–4586 (2013).CAS 
PubMed 
PubMed Central 

Google Scholar 
McGann, P. et al. Detection of New Delhi metallo-β-lactamase (encoded by blaNDM-1) in Acinetobacter schindleri during routine surveillance. J. Clin. Microbiol. 51, 1942–1944 (2013).CAS 
PubMed 
PubMed Central 

Google Scholar 
Forsberg, K. J. et al. The shared antibiotic resistome of soil bacteria and human pathogens. Science 337, 1107–1111 (2012).CAS 
PubMed 
PubMed Central 

Google Scholar 
Jiang, X. et al. Dissemination of antibiotic resistance genes from antibiotic producers to pathogens. Nat. Commun. 8, 15784 (2017).CAS 
PubMed 
PubMed Central 

Google Scholar 
Spanogiannopoulos, P., Waglechner, N., Koteva, K. & Wright, G. D. A rifamycin inactivating phosphotransferase family shared by environmental and pathogenic bacteria. Proc. Natl Acad. Sci. USA 111, 7102–7107 (2014).CAS 
PubMed 
PubMed Central 

Google Scholar 
Yang, J. et al. Marine sediment bacteria harbor antibiotic resistance genes highly similar to those found in human pathogens. Microb. Ecol. 65, 975–981 (2013).CAS 

Google Scholar 
D’Costa, V. M. et al. Antibiotic resistance is ancient. Nature 477, 457–461 (2011).PubMed 
PubMed Central 

Google Scholar 
Van Goethem, M. W. et al. A reservoir of ‘historical’ antibiotic resistance genes in remote pristine Antarctic soils. Microbiome 6, 40 (2018).PubMed 
PubMed Central 

Google Scholar 
Mindlin, S., Soina, V. S., Petrova, M. A. & Gorlenko, Zh. M. Isolation of antibiotic resistance bacterial strains from Eastern Siberia permafrost sediments. Genetika 44, 36–44 (2008).CAS 

Google Scholar 
Cohen, S. N. Transposable genetic elements and plasmid evolution. Nature 263, 731–738 (1976).CAS 

Google Scholar 
Wright, G. D. Environmental and clinical antibiotic resistomes, same only different. Curr. Opin. Microbiol. 51, 57–63 (2019).CAS 

Google Scholar 
von Wintersdorff, C. J. et al. Dissemination of antimicrobial resistance in microbial ecosystems through horizontal gene transfer. Front. Microbiol. 7, 173 (2016).PubMed 
PubMed Central 

Google Scholar 
Rankin, D. J., Rocha, E. P. C. & Brown, S. P. What traits are carried on mobile genetic elements, and why? Heredity (Edinb) https://doi.org/10.1038/hdy.2010.24 (2011).Kottara, A., Hall, J. P., Harrison, E. & Brockhurst, M. A. Variable plasmid fitness effects and mobile genetic element dynamics across Pseudomonas species. FEMS Microbiol. Ecol. 94, fix172 (2018).
Google Scholar 
Hall, J. P., Wood, A. J., Harrison, E. & Brockhurst, M. A. Source–sink plasmid transfer dynamics maintain gene mobility in soil bacterial communities. Proc. Natl Acad. Sci. USA 113, 8260–8265 (2016).CAS 
PubMed 
PubMed Central 

Google Scholar 
Hall, J. P. J., Williams, D., Paterson, S., Harrison, E. & Brockhurst, M. A. Positive selection inhibits gene mobilisation and transfer in soil bacterial communities. Nat. Ecol. Evol. 1, 1348–1353 (2017).PubMed 
PubMed Central 

Google Scholar 
Naumann, T. A. & Reznikoff, W. S. Tn5 transposase with an altered specificity for transposon ends. J. Bacteriol. 184, 233–240 (2002).CAS 
PubMed 
PubMed Central 

Google Scholar 
Wang, H. et al. Increased plasmid copy number is essential for Yersinia T3SS function and virulence. Science 353, 492–495 (2016).CAS 

Google Scholar 
Sandegren, L. & Andersson, D. I. Bacterial gene amplification: implications for the evolution of antibiotic resistance. Nat. Rev. Microbiol. 7, 578–588 (2009).CAS 

Google Scholar 
Dimitriu, T., Mathews, A. C. & Buckling, A. Increased copy number couples the evolution of plasmid horizontal transmission and plasmid-encoded antibiotic resistance. Proc. Natl Acad. Sci. USA 118, e2107818118 (2021).CAS 
PubMed 
PubMed Central 

Google Scholar 
De Lorenzo, V., Herrero, M., Jakubzik, U. & Timmis, K. N. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. J. Bacteriol. 172, 6568–6572 (1990).CAS 
PubMed 
PubMed Central 

Google Scholar 
Lichtenstein, C. & Brenner, S. Site-specific properties of Tn7 transposition into the E. coli chromosome. Mol. Gen. Genet. 183, 380–387 (1981).CAS 

Google Scholar 
Bethke, J. H. et al. Environmental and genetic determinants of plasmid mobility in pathogenic Escherichia coli. Sci. Adv. 6, eaax3173 (2020).CAS 
PubMed 
PubMed Central 

Google Scholar 
Mahillon, J. & Chandler, M. Insertion sequences. Microbiol. Mol. Biol. Rev. 62, 725–774 (1998).CAS 
PubMed 
PubMed Central 

Google Scholar 
Siguier, P., Perochon, J., Lestrade, L., Mahillon, J. & Chandler, M. ISfinder: the reference centre for bacterial insertion sequences. Nucleic Acids Res. 34, D32–D36 (2006).CAS 

Google Scholar 
Seelke, R. W., Kline, B. C., Trawick, J. D. & Ritts, G. D. Genetic studies of F plasmid maintenance genes involved in copy number control, incompatability, and partitioning. Plasmid 7, 163–179 (1982).CAS 

Google Scholar 
Baba, T. et al. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2, 2006.0008 (2006).PubMed 
PubMed Central 

Google Scholar 
Watve, M. M., Dahanukar, N. & Watve, M. G. Sociobiological control of plasmid copy number in bacteria. PLoS ONE 5, e9328 (2010).PubMed 
PubMed Central 

Google Scholar 
Lehtinen, S. et al. Horizontal gene transfer rate is not the primary determinant of observed antibiotic resistance frequencies in Streptococcus pneumoniae. Sci. Adv. 6, eaaz6137 (2020).CAS 
PubMed 
PubMed Central 

Google Scholar 
Ubeda, C. et al. Antibiotic-induced SOS response promotes horizontal dissemination of pathogenicity island-encoded virulence factors in staphylococci. Mol. Microbiol. 56, 836–844 (2005).CAS 

Google Scholar 
Beaber, J. W., Hochhut, B. & Waldor, M. K. SOS response promotes horizontal dissemination of antibiotic resistance genes. Nature 427, 72–74 (2004).CAS 

Google Scholar 
al‐Masaudi, S. B., Day, M. & Russell, A. D. Effect of some antibiotics and biocides on plasmid transfer in Staphylococcus aureus. J. Appl. Bacteriol. 71, 239–243 (1991).
Google Scholar 
Nichols, B. P. & Guay, G. G. Gene amplification contributes to sulfonamide resistance in Escherichia coli. Antimicrob. Agents Chemother. 33, 2042–2048 (1989).CAS 
PubMed 
PubMed Central 

Google Scholar 
Normark, S., Edlund, T., Grundström, T., Bergström, S. & Wolf-Watz, H. Escherichia coli K-12 mutants hyperproducing chromosomal beta-lactamase by gene repetitions. J. Bacteriol. 132, 912–922 (1977).CAS 
PubMed 
PubMed Central 

Google Scholar 
Zienkiewicz, M., Kern-Zdanowicz, I., Carattoli, A., Gniadkowski, M. & Cegłowski, P. Tandem multiplication of the IS 26-flanked amplicon with the blaSHV-5 gene within plasmid p1658/97. FEMS Microbiol. Lett. 341, 27–36 (2013).CAS 
PubMed 
PubMed Central 

Google Scholar 
Matthews, P. R. & Stewart, P. R. Amplification of a section of chromosomal DNA in methicillin-resistant Staphylococcus aureus following growth in high concentrations of methicillin. J. Gen. Microbiol. 134, 1455–1464 (1988).CAS 
PubMed 
PubMed Central 

Google Scholar 
Sun, S., Berg, O. G., Roth, J. R. & Andersson, D. I. Contribution of gene amplification to evolution of increased antibiotic resistance in Salmonella typhimurium. Genetics 182, 1183–1195 (2009).CAS 
PubMed 
PubMed Central 

Google Scholar 
Andersson, D. I. & Hughes, D. Gene amplification and adaptive evolution in bacteria. Annu. Rev. Genet. 43, 167–195 (2009).CAS 

Google Scholar 
Nicoloff, H., Perreten, V. & Levy, S. B. Increased genome instability in Escherichia coli lon mutants: relation to emergence of multiple-antibiotic-resistant (Mar) mutants caused by insertion sequence elements and large tandem genomic amplifications. Antimicrob. Agents Chemother. 51, 1293–1303 (2007).CAS 
PubMed 
PubMed Central 

Google Scholar 
Bertini, A. et al. Multicopy blaOXA-58 gene as a source of high-level resistance to carbapenems in Acinetobacter baumannii. Antimicrob. Agents Chemother. 51, 2324–2328 (2007).CAS 
PubMed 
PubMed Central 

Google Scholar 
Knapp, C. W. et al. Indirect evidence of transposon-mediated selection of antibiotic resistance genes in aquatic systems at low-level oxytetracycline exposures. Environ. Sci. Technol. 42, 5348–5353 (2008).CAS 

Google Scholar 
San Millan, A., Escudero, J. A., Gifford, D. R., Mazel, D. & MacLean, R. C. Multicopy plasmids potentiate the evolution of antibiotic resistance in bacteria. Nat. Ecol. Evol. 1, 10 (2016).
Google Scholar 
Rodriguez-Beltran, J. et al. Multicopy plasmids allow bacteria to escape from fitness trade-offs during evolutionary innovation. Nat. Ecol. Evol. 2, 873–881 (2018).PubMed 
PubMed Central 

Google Scholar 
Rodríguez-Beltrán, J., DelaFuente, J., León-Sampedro, R., MacLean, R. C. & San Millán, Á. Beyond horizontal gene transfer: the role of plasmids in bacterial evolution. Nat. Rev. Microbiol. 19, 347–359 (2021).
Google Scholar 
Frost, L. S., Leplae, R., Summers, A. O. & Toussaint, A. Mobile genetic elements: the agents of open source evolution. Nat. Rev. Microbiol. 3, 722–732 (2005).CAS 

Google Scholar 
You, L., Hoonlor, A. & Yin, J. Modeling biological systems using Dynetica—a simulator of dynamic networks. Bioinformatics 19, 435–436 (2003).CAS 

Google Scholar 
Afgan, E. et al. The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update. Nucleic Acids Res. 46, W537–W544 (2018).CAS 
PubMed 
PubMed Central 

Google Scholar 
Wingett, S. W. & Andrews, S. FastQ Screen: a tool for multi-genome mapping and quality control. F1000Res. 7, 1338 (2018).PubMed 
PubMed Central 

Google Scholar 
Blankenberg, D. et al. Manipulation of FASTQ data with Galaxy. Bioinformatics 26, 1783–1785 (2010).CAS 
PubMed 
PubMed Central 

Google Scholar 
Magoč, T. & Salzberg, S. L. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics 27, 2957–2963 (2011).PubMed 
PubMed Central 

Google Scholar 
Smith, T., Heger, A. & Sudbery, I. UMI-tools: modeling sequencing errors in unique molecular identifiers to improve quantification accuracy. Genome Res. 27, 491–499 (2017).CAS 
PubMed 
PubMed Central 

Google Scholar 
Langmead, B., Trapnell, C., Pop, M. & Salzberg, S. L. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 10, R25 (2009).Langmead, B. & Salzberg, S. L. Fast gapped-read alignment with Bowtie 2. Nat. Methods 9, 357–359 (2012).CAS 
PubMed 
PubMed Central 

Google Scholar 
Li, H. et al. The sequence alignment/map format and SAMtools. Bioinformatics 25, 2078–2079 (2009).PubMed 
PubMed Central 

Google Scholar  More

Caldwell, M. W. “Without a leg to stand on”: on the evolution and development of axial elongation and limblessness in tetrapods. Can. J. Earth Sci. 40, 573–588 (2003).
Google Scholar 
Bejder, L. & Hall, B. K. Limbs in whales and limblessness in other vertebrates: mechanisms of evolutionary and developmental transformation and loss. Evol. Dev. 4, 445–458 (2002).PubMed 

Google Scholar 
Gans, C. Locomotion and burrowing in limbless vertebrates. Nature 242, 414–415 (1973).
Google Scholar 
Gans, C. Tetrapod limblessness: evolution and functional corollaries. Am. Zool. 15, 455–467 (1975).
Google Scholar 
Camaiti, M., Evans, A. R., Hipsley, C. A. & Chapple, D. G. A farewell to arms and legs: a review of limb reduction in squamates. Biol. Rev. 96, 1035–1050 (2021).PubMed 

Google Scholar 
Brandley, M. C., Huelsenbeck, J. P. & Wiens, J. J. Rates and patterns in the evolution of snake‐like body form in squamate reptiles: evidence for repeated re‐evolution of lost digits and long‐term persistence of intermediate body forms. Evol. Int. J. Org. Evol. 62, 2042–2064 (2008).
Google Scholar 
Skinner, A., Lee, M. S. & Hutchinson, M. N. Rapid and repeated limb loss in a clade of scincid lizards. BMC Evol. Biol. 8, 310 (2008).Marjanović, D. & Laurin, M. Phylogeny of Paleozoic limbed vertebrates reassessed through revision and expansion of the largest published relevant data matrix. PeerJ 6, e5565 (2019).PubMed 
PubMed Central 

Google Scholar 
Woltering, J. M. et al. Axial patterning in snakes and caecilians: evidence for an alternative interpretation of the Hox code. Dev. Biol. 332, 82–89 (2009).CAS 
PubMed 

Google Scholar 
Cohn, M. J. & Tickle, C. Developmental basis of limblessness and axial patterning in snakes. Nature 399, 474–479 (1999).CAS 
PubMed 

Google Scholar 
Jaekel, O. Über die klassen der tetrapoden [About the classes of the tetrapods]. Zool. Anz. 34, 193–212 (1909).
Google Scholar 
Anderson J. S. in Major Transitions in Vertebrate Evolution (eds Anderson, J. S. & Sues, H.-D.) 182–227 (Indiana Univ. Press, 2007).Cope, E. D. Synopsis of the extinct Batrachia from the Coal Measures. Ohio Geol. Surv. 2, 349–411 (1875).
Google Scholar 
Farrell, Ú. Pyritization of soft tissues in the fossil record: an overview. Paleontol. Soc. Pap. 20, 35–58 (2014).
Google Scholar 
Mann, A. Cranial ornamentation of a large Brachydectes newberryi (Recumbirostra: Lysorophia) from Linton, Ohio. Vertebr. Anat. Morphol. Palaeontol. 6, 91–96 (2018).
Google Scholar 
Mann, A., Pardo, J. D. & Maddin, H. C. Infernovenator steenae, a new serpentine recumbirostran from the ‘Mazon Creek’ Lagerstätte further clarifies lysorophian origins. Zool. J. Linn. Soc. 187, 506–517 (2019).
Google Scholar 
Maisano, J. A. A survey of state of ossification in neonatal squamates. Herpetol. Monogr. 15, 135–157 (2001).Maisano, J. A. Terminal fusions of skeletal elements as indicators of maturity in squamates. J. Vertebr. Paleontol. 22, 268–275 (2002).
Google Scholar 
Maisano, J. A. Terminal fusions of skeletal elements as indicators of maturity in squamates. J. Vertebr. Paleontol. 22, 268–275 (2002).
Google Scholar 
Pardo, J. D. & Anderson, J. S. Cranial morphology of the Carboniferous–Permian tetrapod Brachydectes newberryi (Lepospondyli, Lysorophia): new data from µCT. PLoS ONE 11, e0161823 (2016).PubMed 
PubMed Central 

Google Scholar 
Milner, A. R. Small temnospondyl amphibians from the Middle Pennsylvanian of Illinois. Paleontology 25, 635–664 (1982).
Google Scholar 
Godfrey, S. A diminutive temnospondyl amphibian from the Pennsylvanian of Illinois. Can. J. Earth Sci. 40, 507–514 (2003).
Google Scholar 
Mann, A. & Maddin, H. C. Diabloroter bolti, a short-bodied recumbirostran ‘microsaur’ from the Francis Creek Shale, Mazon Creek, Illinois. Zool. J. Linn. Soc. 187, 494–505 (2019).
Google Scholar 
Mann, A., McDaniel, E. J., McColville, E. R. & Maddin, H. C. Carbonodraco lundi gen et sp. nov., the oldest parareptile, from Linton, Ohio, and new insights into the early radiation of reptiles. R. Soc. Open Sci. 6, 191191 (2019).PubMed 
PubMed Central 

Google Scholar 
Mann, A. & Gee, B. M. Lissamphibian-like toepads in an exceptionally preserved amphibamiform from Mazon Creek. J. Vertebr. Paleontol. 39, e1727490 (2020).
Google Scholar 
Wellstead, C. F. Taxonomic revision of the Lysorophia, Permo-Carboniferous lepospondyl amphibians. Bull. Am. Mus. Nat. Hist. 209, 1–90 (1991).
Google Scholar 
Sallan, L. C. & Coates, M. I. The long-rostrumed elasmobranch Bandringa Zangerl, 1969, and taphonomy within a Carboniferous shark nursery. J. Vertebr. Paleontol. 34, 22–33 (2014).
Google Scholar 
Allison, P. A. & Briggs, D. E. Exceptional fossil record: distribution of soft-tissue preservation through the Phanerozoic. Geology 21, 527–530 (1993).
Google Scholar 
Briggs, D. E. The role of decay and mineralization in the preservation of soft-bodied fossils. Annu. Rev. Earth Planet. Sci. 31, 275–301 (2003).CAS 

Google Scholar 
Rieppel, O. Studies on skeleton formation in reptiles. V. Patterns of ossification in the skeleton of Alligator mississippiensis Daudin (Reptilia, Crocodylia). Zool. J. Linn. Soc. 109, 301–325 (1993).
Google Scholar 
Sheil, C. A. Skeletal development of Macrochelys temminckii (Reptilia: Testudines: Chelydridae). J. Morphol. 263, 71–106 (2005).PubMed 

Google Scholar 
Roscito, J. G. & Rodrigues, M. T. Skeletal development in the fossorial gymnophthalmids Calyptommatus sinebrachiatus and Nothobachia ablephara. Zoology 115, 289–301 (2012).PubMed 

Google Scholar 
Boisvert, C. A. Vertebral development of modern salamanders provides insights into a unique event of their evolutionary history. J. Exp. Zool. B 312, 1–29 (2009).
Google Scholar 
Klembara, J. & Janiga, M. Variation in Discosauriscus austriacus (Makowsky, 1876) from the Lower Permian of the Boskovice Furrow (Czech Republic). Zool. J. Linn. Soc. 108, 247–270 (1993).
Google Scholar 
Pardo, J. D., Szostakiwskyj, M., Ahlberg, P. E. & Anderson, J. S. Hidden morphological diversity among early tetrapods. Nature 546, 642–645 (2017).CAS 
PubMed 

Google Scholar 
Mann, A., Calthorpe, A. S. & Maddin, H. C. Joermungandr bolti, an exceptionally preserved ‘microsaur’ from the Mazon Creek Lagerstätte reveals patterns of integumentary evolution in Recumbirostra. R. Soc. Open Sci. 8, 210319 (2021).PubMed 
PubMed Central 

Google Scholar 
Swofford, D. Phylogenetic analysis using parsimony (PAUP) v.4.0b10 (Sinauer Associates, 2002).Cohn, M. J. & Bright, P. E. Molecular control of vertebrate limb development, evolution and congenital malformations. Cell Tissue Res. 296, 3–17 (1999).CAS 
PubMed 

Google Scholar 
Mizuhashi, K. et al. Resting zone of the growth plate houses a unique class of skeletal stem cells. Nature 563, 254–258 (2018).CAS 
PubMed 
PubMed Central 

Google Scholar 
Marchini, M. & Rolian, C. Artificial selection sheds light on developmental mechanisms of limb elongation. Evolution 72, 825–837 (2018).PubMed 

Google Scholar 
Rolian, C. Endochondral ossification and the evolution of limb proportions. WIREs Dev. Biol. 9, e373 (2020).Weir, E. C. et al. Targeted overexpression of parathyroid hormone-related peptide in chondrocytes causes chondrodysplasia and delayed endochondral bone formation. Proc. Natl Acad. Sci. USA 93, 10240–10245 (1996).CAS 
PubMed 
PubMed Central 

Google Scholar 
Terpstra, L. et al. Reduced chondrocyte proliferation and chondrodysplasia in mice lacking the integrin-linked kinase in chondrocytes. J. Cell Biol. 162, 139–148 (2003).CAS 
PubMed 
PubMed Central 

Google Scholar 
Marchini, M., Hernandez, E. S. & Rolian, C. Morphology and development of a novel murine skeletal dysplasia. PeerJ 7, e7180 (2019).PubMed 
PubMed Central 

Google Scholar 
Shapiro, M. D., Hanken, J. & Rosenthal, N. Developmental basis of evolutionary digit loss in the Australian lizard Hemiergis. J. Exp. Zool. B 297, 48–56 (2003).
Google Scholar 
Leal, F. & Cohn, M. J. Loss and re-emergence of legs in snakes by modular evolution of Sonic hedgehog and HOXD enhancers. Curr. Biol. 26, 2966–2973 (2016).CAS 
PubMed 

Google Scholar 
Leal, F. & Cohn, M. J. Developmental, genetic, and genomic insights into the evolutionary loss of limbs in snakes. Genesis 56, e23077 (2018).Lande, R. Evolutionary mechanisms of limb loss in tetrapods. Evolution 32, 73–92 (1978).PubMed 

Google Scholar 
Anderson, J. S. Revision of the aïstopod genus Phlegethontia (Tetrapoda: Lepospondyli). J. Paleontol. 76, 1029–1046 (2002).
Google Scholar 
Anderson, J. S. A new aïstopod (Tetrapoda: Lepospondyli) from Mazon Creek, Illinois. J. Vertebr. Paleontol. 23, 79–88 (2003).
Google Scholar 
Shapiro, M. D. Developmental morphology of limb reduction in Hemiergis (Squamata: Scincidae): chondrogenesis, osteogenesis, and heterochrony. J. Morphol. 254, 211–231 (2002).PubMed 

Google Scholar 
Herbst, E. C. & Hutchinson, J. R. New insights into the morphology of the Carboniferous tetrapod Crassigyrinus scoticus from computed tomography. Earth Environ. Sci. Trans. R. Soc. Edinb. 109, 157–175 (2019).CAS 

Google Scholar 
Carroll, R. L. & Gaskill, P. The order Microsauria. Mem. Am. Philos. Soc. 126, 1–211 (1978).
Google Scholar 
Tchernov, E., Rieppel, O., Zaher, H., Polcyn, M. J. & Jacobs, L. L. A fossil snake with limbs. Science 287, 2010–2012 (2000).CAS 
PubMed 

Google Scholar 
Zaher, H., Apesteguia, S. & Scanferla, C. A. The anatomy of the Upper Cretaceous snake Najash rionegrina Apesteguía & Zaher, 2006, and the evolution of limblessness in snakes. Zool. J. Linn. Soc. 156, 801–826 (2009).
Google Scholar 
Jenkins, F. A., Walsh, D. M. & Carroll, R. L. Anatomy of Eocaecilia micropodia, a limbed caecilian of the Early Jurassic. Bull. Mus. Comp. Zool. 158, 285–365 (2007).
Google Scholar 
Camp, C. L. Classification of the lizards. Bull. Am. Mus. Nat. Hist. 48, 289–480 (1923).
Google Scholar 
Essex, R. Studies in reptilian degeneration. Proc. Zool. Soc. Lond. 97, 879–945 (1927).
Google Scholar 
Sewertzoff, A. N. Studien über die reduktion der organe der wirbeltiere. Zool. Jahrb. Abt. Anat. Ontog. Tiere 53, 611–699 (1931).
Google Scholar  More

BioassaysSalmon-louse bioassays were performed by the BC Centre for Aquatic Health Sciences (CAHS) as described in Saksida et al.10. Briefly, motile (i.e., pre-adult and adult) L. salmonis were collected from 11 salmon farms in the Broughton Archipelago (BA) between 2010 and 2021 and transported to CAHS in Campbell River, BC. Within 18 h of collection, healthy lice were separated by sex and randomly placed into petri dishes each containing approximately 10 lice (mean ± SD = 9.6 ± 1.1) and subjected to one of six EMB concentrations (either 0, 31.3, 62.5, 125, 250, and 500 ppb or 0, 62.5, 125, 250, 500, and 1000 ppb, depending on suspected variation in EMB sensitivity11). Each collection corresponded to one bioassay, and each bioassay contained roughly four replicates for each sex (4.0 ± 1.3 for females and 3.6 ± 0.9 for males). After 24 h of EMB exposure, lice were classified as alive if they could swim and attach to the petri dish, or moribund/dead otherwise. Lice were kept at 10 °C throughout the process. In total, 34 bioassays were conducted from 11 farms between October 2010 and November 2021.We analysed the proportion of lice that survived exposure to EMB, using standard statistical descriptions that accounted for within-assay dependencies (generalized linear mixed models (GLMMs) with logit link functions, fitted separately to the data from each bioassay). The models included fixed effects for EMB concentration, sex, and the interaction between the two, as well as a random intercept for petri dish. For each analysis, we centered concentration values and scaled them by one standard deviation. We used the GLMM fits to calculate the effective concentrations at which 50% of the lice survived (EC50) in each bioassay. The GLMM for one bioassay produced a singular fit because there was not enough variation in the female survival data to warrant the random-effects structure. We retained the EC50 values resulting from this singular fit because re-fitting without the random intercept yielded identical EC50 values, and removing the entire bioassay from the overall dataset did not qualitatively affect the subsequent analysis.To assess whether the sensitivity of salmon lice to EMB has decreased over time, we fitted a set of five standard GLMs with gamma error distributions and log link functions to the maximum-likelihood EC50 estimates. Each of these five models included binary effects for sex and for whether the farm’s stock had previously been treated, since both affect EMB sensitivity in lice10. The first model included only these two effects and served as a null model that assumed lice did not evolve EMB resistance over time. The second model added a fixed effect for time (i.e., the number of days since January 1, 2010), while the third model included an interaction between time and sex. The fourth and fifth models were identical to the second and third, but with a quadratic effect for time, to account for possible first-order nonlinearity. We were unable to add an effect for farm due to small sample sizes. We performed model selection using the Akaike Information Criterion penalized for small sample sizes AICc25, treating AICc differences of less than two as being indistinguishable in terms of statistical support and selecting the least complex model when that was the case26. The ΔAICc values for the EC50 models were 48.1, 6.1, 4.9, 0, 1.75, respectively.Field efficacyWe used relative salmon-louse counts after EMB treatment (i.e., the post-treatment count divided by the pre-treatment count) as our measure of EMB field resistance between 2010 and 2021 (higher relative counts imply lower treatment efficacy). We defined “pre-treatment” as one month prior to treatment and “post-treatment” as three months after treatment (roughly when one would expect to find the lowest counts in louse populations previously unexposed to EMB), as in Saksida et al.10. We excluded EMB treatments for which an additional, non-EMB treatment was performed within the following three months. In total, there were 73 EMB treatments for which we were able to calculate relative post-treatment counts.Salmon-louse counts were performed by farm staff as described by Godwin et al.27. In short, salmon-louse counts were usually performed at least one per month by capturing 20 stocked fish in each of three net pens using a box seine net, then placing the fish in an anesthetic bath of tricaine methanesulfonate (TMS, or MS-222) and assessing the fish for motile (i.e., pre-adult and adult) L. salmonis by eye.The treatment dataset included the date and type of every treatment that has been performed on a BA farm (i.e., not just the 11 farms with bioassay data). In total, 88 EMB treatments were conducted between 2010 and 2021, of which we were able to calculate relative post-treatment counts for 73 because some months lacked counts or had a non-EMB treatment performed within the following three months. An additional 22 non-EMB treatments (e.g., freshwater and hydrogen baths) were performed, all since the beginning of 2019, but we excluded these data from our analysis.To determine whether field efficacy of EMB treatments has decreased over time, we used GLM-based “hurdle models”—standard statistical descriptions used to accommodate an over-abundance of zeroes in data being analysed. A hurdle model uses two components—one model for whether a count is nonzero and another for the value of the nonzero count—to predict overall mean count. To this end, we fitted three binomial GLMs paired with three gamma GLMs to the relative-count data, each of the paired models being structurally identical in terms of predictors. All of these submodels included a binary fixed effect for previous treatment, as in the EC50 models. The null pair of submodels included no additional terms, the second pair of submodels included a fixed effect for time (i.e., the number of days since January 1, 2010), and the third pair of submodels included a quadratic effect of time (again, to account for possible first-order deviations nonlinearity). We were unable to add an effect for farm due to small sample sizes. We performed model selection of the hurdle models, again using the Akaike Information Criterion penalized for small sample sizes. The ΔAICc values for the three hurdle models were 39.6, 18.3, and 0, respectively. We performed our analyses in R 3.6.028, using the lme4 package29. More

Connor, E. F. & Taverner, M. P. The evolution and adaptive significance of the leaf-mining habit. Oikos 79, 6–25. https://doi.org/10.2307/3546085 (1997).Article 

Google Scholar 
Hespenheide, H. A. Bionomics of leaf-mining insects. Annu. Rev. Entomol. 36, 535–560. https://doi.org/10.1146/annurev.en.36.010191.002535 (1991).Article 

Google Scholar 
Kato, M. Structure, organization, and response of a species-rich parasitoid community to host leafminer population dynamics. Oecologia 97, 17–25 (1994).ADS 
Article 

Google Scholar 
López, R., Carmona, D., Vincini, A. M., Monterubbianesi, G. & Caldiz, D. Population dynamics and damage caused by the leafminer Liriomyza huidobrensis Blanchard (Diptera: Agromyzidae), on seven potato processing varieties grown in temperate environment. Neotrop. Entomol. 39, 108–114. https://doi.org/10.1590/S1519-566X2010000100015 (2010).Article 
PubMed 

Google Scholar 
Lopez-Vaamonde, C., Godfray, H. C. J. & Cook, J. M. Evolutionary dynamics of host-plant use in a genus of leaf-mining moths. Evolution 57, 1804–1821. https://doi.org/10.1111/j.0014-3820.2003.tb00588.x (2003).Article 
PubMed 

Google Scholar 
Lopez-Vaamonde, C. et al. Fossil-calibrated molecular phylogenies reveal that leaf-mining moths radiated millions of years after their host plants. J. Evol. Biol. 19, 1314–1326. https://doi.org/10.1111/j.1420-9101.2005.01070.x (2006).CAS 
Article 
PubMed 

Google Scholar 
Scheffer, S. J., Lewis, M. L., Hébert, J. B. & Jacobsen, F. Diversity and host plant-use in North American Phytomyza Holly Leafminers (Diptera: Agromyzidae): Colonization, divergence, and specificity in a host-associated radiation. Ann. Entomol. Soc. Am. 114, 59–69. https://doi.org/10.1093/aesa/saaa034 (2021).CAS 
Article 

Google Scholar 
Tooker, J. F. & Giron, D. The evolution of endophagy in herbivorous insects. Front. Plant Sci. 11, 581816. https://doi.org/10.3389/fpls.2020.581816 (2020).Article 
PubMed 
PubMed Central 

Google Scholar 
Hawkins, B. A. Pattern and Process in Host-Parasitoid Interactions (Cambridge University Press, 1994).Book 

Google Scholar 
Novotny, V. & Basset, Y. Host specificity of insect herbivores in tropical forests. Proc. R. Soc. B Biol. Sci. 272, 1083–1090. https://doi.org/10.1098/rspb.2004.3023 (2005).Article 

Google Scholar 
Lewis, O. T. et al. Structure of a diverse tropical forest insect-parasitoid community. J. Anim. Ecol. 71, 855–873. https://doi.org/10.1046/j.1365-2656.2002.00651.x (2002).Article 

Google Scholar 
Hirao, T. & Murakami, M. Quantitative food webs of lepidopteran leafminers and their parasitoids in a Japanese deciduous forest. Ecol. Res. 23, 159–168. https://doi.org/10.1007/s11284-007-0351-6 (2008).Article 

Google Scholar 
Pocock, M. J. O., Evans, D. M. & Memmott, J. The robustness and restoration of a network of ecological networks. Science 335, 973–977. https://doi.org/10.1126/science.1214915 (2012).ADS 
CAS 
Article 
PubMed 

Google Scholar 
Leppänen, S. A., Altenhofer, E., Liston, A. D. & Nyman, T. Phylogenetics and evolution of host-plant use in leaf-mining sawflies (Hymenoptera: Tenthredinidae: Heterarthrinae). Mol. Phylogenet. Evol. 64, 331–341. https://doi.org/10.1016/j.ympev.2012.04.005 (2012).Article 
PubMed 

Google Scholar 
Doorenweerd, C., Van Nieukerken, E. J. & Menken, S. B. J. A global phylogeny of leafmining Ectoedemia moths (Lepidoptera: Nepticulidae): Exploring host plant family shifts and allopatry as drivers of speciation. PLoS ONE 10, 1–20. https://doi.org/10.1371/journal.pone.0119586 (2015).CAS 
Article 

Google Scholar 
Nakadai, R. & Kawakita, A. Phylogenetic test of speciation by host shift in leaf cone moths (Caloptilia) feeding on maples (Acer). Ecol. Evol. 6, 4958–4970. https://doi.org/10.1002/ece3.2266 (2016).Article 
PubMed 
PubMed Central 

Google Scholar 
Opler, P. A. Fossil lepidopterous leaf mines demonstrate the age of some insect-plant relationships. Science 179, 1321–1323. https://doi.org/10.1126/science.179.4080.1321 (1973).ADS 
CAS 
Article 
PubMed 

Google Scholar 
Labandeira, C. C., Dilcher, D. L., Davis, D. R. & Wagner, D. L. Ninety-seven million years of angiosperm-insect association: Paleobiological insights into the meaning of coevolution. Proc. Natl. Acad. Sci. U. S. A. 91, 12278–12282. https://doi.org/10.1073/pnas.91.25.12278 (1994).ADS 
CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Winkler, I. S., Labandeira, C. C., Wappler, T. & Wilf, P. Distinguishing Agromyzidae (Diptera) leaf mines in the fossil record: New taxa from the Paleogene of North America and Germany and their evolutionary implications. J. Paleontol. 84, 935–954. https://doi.org/10.1666/09-163.1 (2010).Article 

Google Scholar 
van Nieukerken, E. J., Doorenweerd, C., Hoare, R. J. B. & Davis, D. R. Revised classification and catalogue of global Nepticulidae and Opostegidae (Lepidoptera, Nepticuloidea). Zookeys 2016, 65–246. https://doi.org/10.3897/zookeys.628.9799 (2016).Article 

Google Scholar 
Maccracken, S. A., Sohn, J.-C., Miller, I. M. & Labandeira, C. C. A new Late Cretaceous leaf mine Leucopteropsa spiralae gen. et sp. nov. (Lepidoptera: Lyonetiidae) represents the first confirmed fossil evidence of the Cemiostominae. J. Syst. Palaeontol. 19, 131–144. https://doi.org/10.1080/14772019.2021.1881177 (2021).Article 

Google Scholar 
Wilf, P., Labandeira, C. C., Johnson, K. R. & Ellis, B. Decoupled plant and insect diversity after the end-Cretaceous extinction. Science 313, 1112–1115. https://doi.org/10.1126/science.1129569 (2006)ADS 
CAS 
Article 
PubMed 

Google Scholar 
Donovan, M. P., Wilf, P., Labandeira, C. C., Johnson, K. R. & Peppe, D. J. Novel insect leaf-mining after the end-Cretaceous extinction and the demise of Cretaceous leaf miners, Great Plains, USA. PLoS ONE 9, e103542. https://doi.org/10.1371/journal.pone.0103542 (2014).ADS 
CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Donovan, M. P., Iglesias, A., Wilf, P., Labandeira, C. C. & Cúneo, N. R. Rapid recovery of Patagonian plant–insect associations after the end-Cretaceous extinction. Nat. Ecol. Evol. 1, 0012. https://doi.org/10.1038/s41559-016-0012 (2017).Article 

Google Scholar 
Donovan, M. P., Wilf, P., Iglesias, A., Cúneo, N. R. & Labandeira, C. C. Persistent biotic interactions of a Gondwanan conifer from Cretaceous Patagonia to modern Malesia. Commun. Biol. 3, 708. https://doi.org/10.1038/s42003-020-01428-9 (2020).Article 
PubMed 
PubMed Central 

Google Scholar 
Labandeira, C. C. The four phases of plant-arthropod associations in deep time. Geol. Acta 4, 409–438. https://doi.org/10.1344/105.000000344 (2006).Article 

Google Scholar 
Labandeira, C. C. Silurian to Triassic plant and hexapod clades and their associations: new data, a review, and interpretations. Arthropod Syst. Phylogen. 64, 53–94 (2006).
Google Scholar 
Wakita, K., Nakagawa, T., Sakata, M., Tanaka, N. & Oyama, N. Phanerozoic accretionary history of Japan and the western Pacific margin. Geol. Mag. https://doi.org/10.1017/s0016756818000742 (2018).Article 

Google Scholar 
Katayama, M. Stratigraphical study on the Mine Series. J. Geol. Soc. Jpn. 46, 127–141. https://doi.org/10.5575/geosoc.46.127 (1939).Article 

Google Scholar 
Maeda, H. & Oyama, N. Stratigraphy and fossil assemblages of the Triassic Mine Group and Jurassic Toyora Group in western Yamaguchi Prefecture. J. Geol. Soc. Japan 125, 585–594. https://doi.org/10.5575/geosoc.2019.0020 (2019).Article 

Google Scholar 
Aizawa, J. Fossil insect-bearing strata of the Triassic Mine Group, Yamaguchi Prefecture. Bull. Kitakyushu Mus. Nat. Hist. Hum. Hist. Ser. A 10, 91–98 (1991).
Google Scholar 
Oyama, N. & Maeda, H. Madygella humioi sp. nov. from the Upper Triassic Mine Group, Southwest Japan: The oldest record of a sawfly (Hymenoptera: Symphyta) in East Asia. Paleontol. Res. 24, 64–71 (2020).Article 

Google Scholar 
Fujiyama, I. Mesozoic insect fauna of East Asia part 1. Introduction and upper Triassic faunas. Bull. Natl. Sci. Mus. 16, 331–386 (1973).
Google Scholar 
Fujiyama, I. Late Triassic insects from Mine, Yamaguchi, Japan, Part 1. Odonata. Bull. Natl. Sci. Mus. Tokyo Ser. C 17, 49–56 (1991).
Google Scholar 
Ueda, K. A Triassic fossil of scorpion fly from Mine, Japan. Bull. Kitakyushu Mus. Nat. Hist. Hum. Hist. Ser. Ser. A 10, 99–103 (1991).
Google Scholar 
Takahashi, F., Ishida, H., Nohara, M., Doi, E. & Taniguchi, S. Occurrence of insect fossils from the Late Triassic Mine Group. Bull. Mine City Mus. Yamaguchi Prefect. Jpn. 13, 1–27 (1997).CAS 

Google Scholar 
Kametaka, M. Provenance of the Upper Triassic mine group Southwest Japan. J. Geol. Soc. Jpn. 105, 651–667 (1999).CAS 
Article 

Google Scholar 
Takahashi, E. & Mikami, T. Triassic. In Geology of Yamaguchi Prefecture (ed. Yamaguchi Museum) 93–108 (Yamaguchi Museum, 1975).Kiminami, K. Atsu Group and Mine Group. In Monograph on Geology of Japan 6, Chugoku Region (ed. Geological Society of Japan) 85–88 (Asakura Publishing Co., Ltd., 2009).Naito, G. Plant Fossils from the Mine Group (Mine City Education Comittee, 2000).
Google Scholar 
Kimura, T. Geographical distribution of Palaeozoic and Mesozoic plants in East and Southeast Asia. Hist. Biogeogr. Plate Tecton. Evol. Jpn. East Asia 1982, 135–200 (1987).
Google Scholar 
Kimura, T., Naito, G. & Ohana, T. Baiera cf. furcata (Lindley and Hutton) Braun from the Carnic Momonoki Formation, Japan. Bull. Natl. Sci. Mus. 9, 91–114 (1983).
Google Scholar 
Katagiri, T. Pallaviciniites oishii (comb. Nov.), a thalloid liverwort from the Late Triassic of Japan. Bryologist 118, 245–251. https://doi.org/10.1639/0007-2745-118.3.245 (2015).Article 

Google Scholar 
Kustatscher, E. et al. Flora of the Late Triassic. In The Late Triassic World, Topics in Geobiology, Vol. 46 (ed. Tanner, L. H.) 545–622 (Springer, 2018). https://doi.org/10.1007/978-3-319-68009-5_13.Oyama, N., Yukawa, H. & Maeda, H. Mesozoic insect fossils of Japan: Significance of the Upper Triassic insect fauna of the Mine Group, Yamaguchi Pref. Bull. Mine City Mus. Yamaguchi Prefect. Jpn. 33, 1–13 (2020).
Google Scholar 
Shcherbakov, D. E., Lukashevich, E. D. & Blagoderov, V. Triassic Diptera and initial radiation of the order. Int. J. Dipterol. Res. 6, 75–115 (1995).
Google Scholar 
Krzemiński, W. & Krzemińska, E. Triassic Diptera: Descriptions, revisions and phylogenetic relations. Acta Zool. Cracov. 46, 153–184 (2003).
Google Scholar 
Blagoderov, V., Grimaldi, D. A. & Fraser, N. C. How time flies for flies: Diverse Diptera from the Triassic of Virginia and early radiation of the order. Am. Mus. Novit. 3572, 1–39. https://doi.org/10.1206/0003-0082(2007)509[1:HTFFFD]2.0.CO;2 (2007).Article 

Google Scholar 
Lukashevich, E. D., Przhiboro, A. A., Marchal-Papier, F. & Grauvogel-Stamm, L. The oldest occurrence of immature Diptera (Insecta), Middle Triassic France. Ann. la Société Entomol. Fr. 46, 4–22. https://doi.org/10.1080/00379271.2010.10697636 (2010).Article 

Google Scholar 
Schmidt, A. R. et al. Arthropods in amber from the Triassic Period. Proc. Natl. Acad. Sci. 109, 14796–14801. https://doi.org/10.1073/pnas.1208464109 (2012).ADS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Lara, M. B. & Lukashevich, E. D. The first Triassic dipteran (Insecta) from South America, with review of Hennigmatidae. Zootaxa 3710, 81–92. https://doi.org/10.11646/zootaxa.3710.1.6 (2013).Article 
PubMed 

Google Scholar 
Kimura, T. & Ohana, T. Some fossil ferns from the Middle Carnic Momonoki Formation, Yamaguchi prefecture, Japan. Bull. Natl. Sci. Mus. Ser. C Geol. Paleontol. 6, 73–92 (1980).
Google Scholar 
Hering, E. M. Biology of the Leaf Miners https://doi.org/10.1007/978-94-015-7196-8. (Springer, 1951).Book 

Google Scholar 
Kirichenko, N. et al. Systematics of Phyllocnistis leaf-mining moths (Lepidoptera, Gracillariidae) feeding on dogwood (Cornus spp.) in Northeast Asia, with the description of three new species. Zookeys 2018, 79–118. https://doi.org/10.3897/zookeys.736.20739 (2018).Article 

Google Scholar 
Cerdeña, J. et al. Phyllocnistis furcata sp. nov.: A new species of leaf-miner associated with Baccharis (Asteraceae) from Southern Peru (Lepidoptera, Gracillariidae). Zookeys 2020, 121–145. https://doi.org/10.3897/zookeys.996.53958 (2020).Article 

Google Scholar 
Elb, P. M., Melo-de-Pinna, G. F. & de Menezes, N. L. Morphology and anatomy of leaf miners in two species of Commelinaceae (Commelina diffusa Burm. F. and Floscopa glabrata (Kunth) Hassk). Acta Bot. Brasilica 24, 283–287. https://doi.org/10.1590/S0102-33062010000100030 (2010).Article 

Google Scholar 
Vasco, A., Moran, R. C. & Ambrose, B. A. The evolution, morphology, and development of fern leaves. Front. Plant Sci. 4, 1–16. https://doi.org/10.3389/fpls.2013.00345 (2013).Article 

Google Scholar 
Eiseman, C. Leafminers of North America. (Charley Eiseman, 2019).Yang, J., Wang, X., Duffy, K. & Dai, X. A preliminary world checklist of fern-mining insects. Biodivers. Data J. 9, e62839. https://doi.org/10.3897/BDJ.9.e62839 (2021).Article 
PubMed 
PubMed Central 

Google Scholar 
Ding, Q., Labandeira, C. C. & Ren, D. Biology of a leaf miner (Coleoptera) on Liaoningocladus boii (Coniferales) from the Early Cretaceous of northeastern China and the leaf-mining biology of possible insect culprit clades. Arthropod Syst. Phylogen. 72, 281–308 (2014).
Google Scholar 
Boucher, S. Revision of the Canadian species of Amauromyza Hendel (Diptera: Agromyzidae). Can. Entomol. 144, 733–757. https://doi.org/10.4039/tce.2012.80 (2012).Article 

Google Scholar 
Scheirs, J., Vandevyvere, I. & De Bruyn, L. Influence of monocotyl leaf anatomy on the feeding pattern of a grass-mining agromyzid (Diptera). Ann. Entomol. Soc. Am. 90, 646–654 (1997).Article 

Google Scholar 
Boucher, S. Leaf-miner flies (Diptera: Agromyzidae). In Encyclopedia of Entomology (ed. Capinera J. L.) 2163–2169 (Springer, 2008). https://doi.org/10.1007/978-1-4020-6359-6.Eiseman, C. S. New rearing records for muscoid leafminers (Diptera: Anthomyiidae, Scathophagidae) in the United States. Proc. Entomol. Soc. Wash. 120, 25–50. https://doi.org/10.4289/0013-8797.120.1.25 (2018).Article 

Google Scholar 
Meikle, A. A. The insects associated with bracken. Agric. Prog. 14, 58–61 (1937).
Google Scholar 
Lawton, J. H. The structure of the arthropod community on bracken. Bot. J. Linn. Soc. 73, 187–216. https://doi.org/10.1111/j.1095-8339.1976.tb02022.x (1976).Article 

Google Scholar 
Lawton, J. H., MacGarvin, M. & Heads, P. A. Effects of altitude on the abundance and species richness of insect herbivores on bracken. J. Anim. Ecol. 56, 147–160. https://doi.org/10.2307/4805 (1987).Article 

Google Scholar 
Cooper-Driver, Gi. A. Insect-fern associations. Entomol. Exp. Appl. 24, 310–316. https://doi.org/10.1111/j.1570-7458.1978.tb02787.x (1978).Article 

Google Scholar 
Eiseman, C. S. Further Nearctic rearing records for phytophagous muscoid flies (Diptera: Anthomyiidae, Scathophagidae). Proc. Entomol. Soc. Washingt. 122, 595–603. https://doi.org/10.4289/0013-8797.122.3.595 (2020).Article 

Google Scholar 
Santos, M. G. & Maia, V. C. A synopsis of fern galls in Brazil. Biota Neotrop. 18, e20180513. https://doi.org/10.1590/1676-0611-BN-2018-0513 (2018).Article 

Google Scholar 
Peters, R. S. et al. Evolutionary history of the Hymenoptera. Curr. Biol. 27, 1013–1018. https://doi.org/10.1016/j.cub.2017.01.027 (2017). CAS 
Article 
PubMed 

Google Scholar 
Ronquist, F. et al. A total-evidence approach to dating with fossils, applied to the early radiation of the Hymenoptera. Syst. Biol. 61, 973–999. https://doi.org/10.1093/sysbio/sys058 (2012).Article 
PubMed 
PubMed Central 

Google Scholar 
Needham, J. G., Frost, S. W. & Tothill, B. H. Leaf-Mining Insects (Waverly Press, 1928).
Google Scholar 
Smith, D. R., Eiseman, C. S., Charney, N. D. & Record, S. A new Nearctic Scolioneura (Hymenoptera, Tenthredinidae) mining leaves of Vaccinium (Ericaceae). J. Hymenopt. Res. 43, 1–8. https://doi.org/10.3897/JHR.43.4546 (2015).Article 

Google Scholar 
Zheng, D. et al. Middle-Late Triassic insect radiation revealed by diverse fossils and isotopic ages from China. Sci. Adv. 4, eaat1380. https://doi.org/10.1126/sciadv.aat1380 (2018).ADS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Zhang, S. Q. et al. Evolutionary history of Coleoptera revealed by extensive sampling of genes and species. Nat. Commun. 9, 1–11. https://doi.org/10.1038/s41467-017-02644-4 (2018).ADS 
CAS 
Article 

Google Scholar 
McKenna, D. D. et al. The evolution and genomic basis of beetle diversity. Proc. Natl. Acad. Sci. 116, 24729–24737. https://doi.org/10.1073/pnas.1909655116 (2019).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Gimmel, M. L. & Ferro, M. L. General overview of saproxylic Coleoptera. In Saproxylic Insects, Zoological Monographs, Vol. 1 (ed. Ulyshen, M. D.) 51–128 (Springer, 2018). https://doi.org/10.1007/978-3-319-75937-1_2.Labandeira, C. C., Anderson, J. M. & Anderson, H. M. Expansion of arthropod herbivory in Late Triassic South Africa: The Molteno Biota, Aasvoëlberg 411 site and developmental biology of a gall. In The Late Triassic World, Topics in Geobiology Vol. 46 (ed. Tanner, L. H.) 623–719 (Springer International Publishing AG, 2018).Chapter 

Google Scholar 
Fiebrig, K. Eine Schaum bildende Käferlarve Pachyschelus spec. (Bupr. Sap.) Die Ausscheidung von Kautschuk aus der Nahrung und dessen Verwertung zu Schutzzwecken (auch bei Rhynchoten). Z. f. Wiss. Insektenbiol. 4, 333–339 (1908).
Google Scholar 
Bruch, C. Metamórfosis de Pachyschelus undularius (Burm.). Physis 3, 30–36 (1917).
Google Scholar 
Hering, E. M. Neotropische Buprestiden-Minen. Arb. Physiol. Angew. Entomol. 9, 241–249 (1942).
Google Scholar 
Kogan, M. Contribuição ao conhecimento da sistemática e biologia de buprestídeos minadores do gênero Pachyschelus Solier, 1833: (Coleoptera, Buprestidae). Mem. Inst. Oswaldo Cruz 61, 429–457 (1963).CAS 
Article 

Google Scholar 
Kawahara, A. Y. et al. Phylogenomics reveals the evolutionary timing and pattern of butterflies and moths. Proc. Natl. Acad. Sci. 116, 22657–22663. https://doi.org/10.1073/pnas.1907847116 (2019).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Van Eldijk, T. J. B. et al. A Triassic-Jurassic window into the evolution of lepidoptera. Sci. Adv. 4, e1701568. https://doi.org/10.1126/sciadv.1701568 (2018).ADS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Sohn, J. C., Labandeira, C. C., Davis, D. & Mitter, C. An annotated catalog of fossil and subfossil Lepidoptera (Insecta: Holometabola) of the world. Zootaxa. https://doi.org/10.11646/zootaxa.3286.1.1 (2012).Doorenweerd, C., Van Nieukerken, E. J., Sohn, J. C. & Labandeira, C. C. A revised checklist of Nepticulidae fossils (Lepidoptera) indicates an Early Cretaceous origin. Zootaxa 3963, 295–334. https://doi.org/10.11646/zootaxa.3963.3.2 (2015).Article 
PubMed 

Google Scholar 
Kawahara, A. Y. et al. A molecular phylogeny and revised higher-level classification for the leaf-mining moth family Gracillariidae and its implications for larval host-use evolution. Syst. Entomol. 42, 60–81. https://doi.org/10.1111/syen.12210 (2017).Article 

Google Scholar 
Mazumdar, J. Phytoliths of pteridophytes. S. Afr. J. Bot. 77, 10–19. https://doi.org/10.1016/j.sajb.2010.07.020 (2011).Article 

Google Scholar 
Trembath-Reichert, E., Wilson, J. P., McGlynn, S. E. & Fischer, W. W. Four hundred million years of silica biomineralization in land plants. Proc. Natl. Acad. Sci. U. S. A. 112, 5449–5454 https://doi.org/10.1073/pnas.1500289112 (2015).ADS 
CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Hunt, J. W., Dean, A. P., Webster, R. E., Johnson, G. N. & Ennos, A. R. A novel mechanism by which silica defends grasses against herbivory. Ann. Bot. 102, 653–656. https://doi.org/10.1093/aob/mcn130 (2008).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Reynolds, O. L., Keeping, M. G. & Meyer, J. H. Silicon-augmented resistance of plants to herbivorous insects: A review. Ann. Appl. Biol. 155, 171–186. https://doi.org/10.1111/j.1744-7348.2009.00348.x (2009).CAS 
Article 

Google Scholar 
Edwards, N. P. et al. Leaf metallome preserved over 50 million years. Metallomics 6, 774–782. https://doi.org/10.1039/C3MT00242J (2014).CAS 
Article 
PubMed 

Google Scholar 
Müller, A. H. Über Hyponome fossiler und rezenter Insekten, erster Beitrag. Freib. Forschungsh. C 366, 7–27 (1982).
Google Scholar 
Beck, A. L. & Labandeira, C. C. Early Permian insect folivory on a gigantopterid-dominated riparian flora from north-central Texas. Palaeogeogr. Palaeoclimatol. Palaeoecol. 142, 139–173. https://doi.org/10.1016/S0031-0182(98)00060-1 (1998).Article 

Google Scholar 
Jarzembowski, E. A. The oldest plant-insect interaction in Croatia: Carboniferous evidence. Geol. Croat. 65(3), 387–392. https://doi.org/10.4154/GC.2012.28 (2002).Article 

Google Scholar 
Donovan, M. P. & Lucas, S. G. Insect herbivory on the Late Pennsylvanian Kinney Brick Quarry Flora, New Mexico, USA. Kinney Brick Quarry Lagerstätte. N. M. Mus. Nat. Hist. Sci. Bull. 84, 193–207 (2021).Potonié, R. Ueber das Rothliegende des Thüringer Waldes. Theil II: Die Flora des Rothliegenden von Thüringen. Abh. Preuss. Geol. Landesanst. 9, 1–298 (1893).
Google Scholar 
Potonié, R. Mitteilungen über mazerierte kohlige Pflanzenfossilien. Z. Bot. 13, 79–88 (1921).Adami-Rodrigues, K. A., Iannuzzi, R. & Pinto, I. D. Permian plant-insect interactions from a Gondwana flora of southern Brazil. Foss. Strat. 51, 106–126 (2004).
Google Scholar 
Krassilov, V. A. & Karasev, E. First evidence of plant–arthropod interaction at the Permian–Triassic boundary in the Volga Basin European Russia. Alavesia 2, 247–252 (2008).
Google Scholar 
Labandeira, C. C., Wilf, P., Johnson, K. & Marsh, F. Guide to insect (and other) damage types on compressed plant fossils. Version 3.0. Smithson. Institution, Washington, DC 25 (2007).Scott, A. C., Anderson, J. M. & Anderson, H. M. Evidence of plant-insect interactions in the Upper Triassic Molteno formation of South Africa. J. Geol. Soc. London. 161, 401–410. https://doi.org/10.1144/0016-764903-118 (2004).Article 

Google Scholar 
Tillyard, R. J. Mesozoic Insects of Queensland No. 9. Orthoptera, and Additions to the Protorthoptera, Odonata, Hemiptera, and Planipennia. Proc. Linn. Soc. N. S. W. 47, 447–470 (1922).
Google Scholar 
Rozefelds, A. C. & Sobbe, I. Problematic insect leaf mines from the Upper Triassic Ipswich Coal Measures of Southeastern Queensland Australia. Alcheringa 11, 51–57 (1987).Article 

Google Scholar 
Wappler, T., Kustatscher, E. & Dellantonio, E. Plant-insect interactions from Middle Triassic (late Ladinian) of Monte Agnello (Dolomites, N-Italy)-Initial pattern and response to abiotic environmental pertubations. PeerJ 2015, e921. https://doi.org/10.7717/peerj.921 (2015).Article 

Google Scholar 
Meller, B., Ponomarenko, A. G., Vasilenko, D. V., Fischer, T. C. & Aschauer, B. First beetle elytra, abdomen (Coleoptera) and a mine trace from Lunz (Carnian, Late Triassic, Lunz-am-See, Austria) and their taphonomical and evolutionary aspects. Palaeontology 54, 97–110. https://doi.org/10.1111/j.1475-4983.2010.01009.x (2011).Article 

Google Scholar 
Vassilenko, D. V. Traces of plant-arthropod interactions from Madygen (Triassic, Kyrgyzstan): Preliminary data. Sovremennaya paleontologia: klassicheskie i noveishie metody 9–16 (2009).Zherikhin, V. V. Insect Trace Fossils. In History of Insects (ed. Rasnitsyn A. P., Quicke, D. L.) 303–324 (Kluwer Academic Publishers, 2010).Schindelin, J. et al. Fiji: An open-source platform for biological-image analysis. Nat. Methods 9, 676–682. https://doi.org/10.1038/nmeth.2019 (2012).CAS 
Article 
PubMed 

Google Scholar 
Wickham, H. ggplot2: Elegant Graphics for Data Analysis (Springer-Verlag, 2016).Book 

Google Scholar  More

Seigel, R. A. & Ford, N. B. Reproductive ecology in Snakes: Ecology and Evolutionary Biology (eds. Seigel, R. A., Collins, J. T. &. Novak, S. S.). 210–252. (MacMillan Publishing, 1987).Kremen, C., Merenlender, A. M. & Murphy, D. D. Ecological monitoring: A vital need for integrated conservation and development programs in the tropics. Conserv. Biol. 8, 388–397 (1994).
Google Scholar 
Shine, R. & Bonnet, X. Snakes: A new ‘model organism’ in ecological research?. Trends Ecol. Evol. 15, 221–222 (2000).CAS 
PubMed 

Google Scholar 
Vilela, B., Villalobos, F., Rodríguez, M. Á. & Terribile, L. C. Body size, extinction risk and knowledge bias in New World snakes. PLoS ONE 9, e113429 (2014).ADS 
PubMed 
PubMed Central 

Google Scholar 
Mathies, T. Reproductive cycles of tropical snakes. in Reproductive Biology and Phylogeny of Snakes (eds. Sever, D. & Aldridge, R.). 523–562. (CRC Press, 2016).Shine, R., Harlow, P. S. & Keogh, J. S. The allometry of life-history traits: Insights from a study of giant snakes (Python reticulatus). J. Zool. 244, 405–414 (1998).
Google Scholar 
Natusch, D. J., Lyons, J. A., Riyanto, A., Khadiejah, S. & Shine, R. Detailed biological data are informative, but robust trends are needed for informing sustainability of wildlife harvesting: A case study of reptile offtake in Southeast Asia. Biol. Conserv. 233, 83–92 (2019).
Google Scholar 
Freeman, A. & Freeman, A. Habitat use in a large rainforest python (Morelia kinghorni) in the wet tropics of north Queensland, Australia. Herpetol. Conserv. Biol. 4, 252–260 (2009).
Google Scholar 
Smith, S. N., Jones, M. D., Marshall, B. M. & Strine, C. T. Native Burmese pythons exhibit site fidelity and preference for aquatic habitats in an agricultural mosaic. Sci. Rep. 11, 7014 (2021).ADS 
CAS 
PubMed 
PubMed Central 

Google Scholar 
Kramer, D. L. & Chapman, M. R. Implications of fish home range size and relocation for marine reserve function. Environ. Biol. Fishes 55, 65–79 (1999).
Google Scholar 
Spong, G. Space use in lions, Panthera leo, in the Selous Game Reserve: Social and ecological factors. Behav. Ecol. Sociobiol. 52, 303–307 (2002).
Google Scholar 
Webb, J. K. & Shine, R. A field study of spatial ecology and movements of a threatened snake species, Hoplocephalus bungaroides. Biol. Conserv. 82, 203–217 (1997).
Google Scholar 
Fearn, S. & Sambono, J. A reliable size record for the scrub python Morelia amethistina (Serpentes: Pythonidae) in north east Queensland. Herpetofauna 30, 2–6 (2000).
Google Scholar 
Grow, D., Wheeler, S. & Clark, B. Reproduction of the Amethystine python Python amethystinus kinghorni at the Oklahoma City Zoo. Int. Zoo Year. 27, 241–244 (1988).
Google Scholar 
Feldman, A. & Meiri, S. Length–mass allometry in snakes. Biol. J. Linn. Soc. 108, 161–172 (2013).
Google Scholar 
Harvey, M. B., Barker, D. G., Ammerman, L. K. & Chippindale, P. T. Systematics of pythons of the Morelia amethistina complex (Serpentes: Boidae) with the description of three new species. Herpetol. Monogr. 14, 139–185 (2000).
Google Scholar 
Fearn, S., Schwarzkopf, L. & Shine, R. Giant snakes in tropical forests: A field study of the Australian scrub python, Morelia kinghorni. Wildl. Res. 32, 193–201 (2005).
Google Scholar 
Natusch, D. J. D., Lyons, J. A. & Shine, R. Rainforest pythons flexibly adjust foraging ecology to exploit seasonal concentrations of prey. J. Zool. 313, 114–123 (2021).
Google Scholar 
Martin, R. W. Field observation of predation on Bennett’s tree-kangaroo (Dendrolagus bennettianus) by an amethystine python (Morelia amethistina). Herpetol. Rev. 26, 74–75 (1995).
Google Scholar 
Natusch, D., Lyons, J., Mears, L. A. & Shine, R. Biting off more than you can chew: Attempted predation on a human by a giant snake (Simalia amethistina). Austral. Ecol. 46, 159–162 (2021).
Google Scholar 
Neldner, V. J. & Clarkson, J. R. Vegetation of Cape York Peninsula. (Department of Environment and Heritage, 1995).Bureau of Meteorology. Climate Data Online. http://www.bom.gov.au/climate/data/. Accessed 17 July 2020 (2020).Whitaker, P. B. & Shine, R. A radiotelemetric study of movements and shelter-site selection by free-ranging brownsnakes (Pseudonaja textilis, Elapidae). Herpetol. Monogr. 17, 130–144 (2003).
Google Scholar 
Harris, S. et al. Home-range analysis using radio-tracking data–A review of problems and techniques particularly as applied to the study of mammals. Mamm. Rev. 20, 97–123 (1990).
Google Scholar 
Fearn, S. & Sambono, J. Some ambush predation postures of the Scrub Python Morelia amethistina (Serpentes: Pythonidae) in north east Queensland. Herpetofauna 30, 39–44 (2000).
Google Scholar 
Caswell, H. Theory and models in ecology: A different perspective. Ecol. Model. 43, 33–44 (1988).
Google Scholar 
Silva, I., Crane, M., Marshall, B. M. & Strine, C. T. Reptiles on the wrong track? Moving beyond traditional estimators with dynamic Brownian bridge movement models. Move. Ecol. 8, 43 (2020).
Google Scholar 
Row, J. R. & Blouin-Demers, G. Kernels are not accurate estimators of home-range size for herpetofauna. Copeia 2006, 797–802 (2006).
Google Scholar 
Newman, P., Dwyer, R. G., Belbin, L. & Campbell, H. A. ZoaTrack—An online tool to analyse and share animal location data: User engagement and future perspectives. Aust. Zool. 41, 12–18. https://zoatrack.org/toolkit/doi (2020).Pearson, D. J. & Shine, R. Expulsion of interperitoneally-implanted radiotransmitters by Australian pythons. Herpetol. Rev. 33, 261–263 (2002).
Google Scholar 
Hale, V. L. et al. Radio transmitter implantation and movement in the wild timber rattlesnake (Crotalus horridus). J. Wildl. Dis. 53, 591–595 (2017).PubMed 

Google Scholar 
Martin, A. E., Jørgensen, D. & Gates, C. C. Costs and benefits of straight versus tortuous migration paths for Prairie Rattlesnakes (Crotalus viridis viridis) in seminatural and human-dominated landscapes. Can. J. Zool. 95, 921–928 (2017).
Google Scholar 
Glaudas, X., Rice, S. E., Clark, R. W. & Alexander, G. J. Male energy reserves, mate-searching activities, and reproductive success: Alternative resource use strategies in a presumed capital breeder. Oecologia 194, 415–425 (2020).ADS 
PubMed 

Google Scholar 
Glaudas, X., Rice, S. E., Clark, R. W. & Alexander, G. J. The intensity of sexual selection, body size and reproductive success in a mating system with male–male combat: is bigger better?. Oikos 129, 998–1011 (2020).
Google Scholar 
Gannon, V. P. J. & Secoy, D. M. Seasonal and daily activity patterns in a Canadian population of the prairie rattlesnake, Crotalus viridus viridis. Can. J. Zool. 63, 86–91 (1985).
Google Scholar 
Heard, G. W., Black, D. & Robertson, P. Habitat use by the inland carpet python (Morelia spilota metcalfei: Pythonidae): Seasonal relationships with habitat structure and prey distribution in a rural landscape. Austral. Ecol. 29, 446–460 (2004).
Google Scholar 
Madsen, T. & Shine, R. Seasonal migration of predators and prey—A study of pythons and rats in tropical Australia. Ecology 77, 149–156 (1996).
Google Scholar 
Graves, B. M. & Duvall, D. Reproduction, rookery use, and thermoregulation in free-ranging, pregnant Crotalus v. viridis. J. Herpetol. 27, 33–41 (1993).
Google Scholar 
Chiaraviglio, M. The effects of reproductive condition on thermoregulation in the Argentina boa constrictor (Boa constrictor occidentalis) (Boidae). Herpetol. Monogr. 20, 172–177 (2006).
Google Scholar 
Smith, C. F., Schuett, G. W., Earley, R. L. & Schwenk, K. The spatial and reproductive ecology of the copperhead (Agkistrodon contortrix) at the northeastern extreme of its range. Herpetol. Monogr. 23, 45–73 (2009).
Google Scholar 
Shine, R. & Fitzgerald, M. Large snakes in a mosaic rural landscape: The ecology of carpet pythons Morelia spilota (Serpentes: Pythonidae) in coastal eastern Australia. Biol. Conserv. 76, 113–122 (1996).
Google Scholar 
Heard, G. W. et al. Canid predation: A potentially significant threat to relic populations of the Inland Carpet Python ‘Morelia spilota metcalfei’ (Pythonidae) in Victoria. Vic. Nat. 123, 68–74 (2006).
Google Scholar 
Downes, S. & Shine, R. Sedentary snakes and gullible geckos: Predator–prey coevolution in nocturnal rock-dwelling reptiles. Anim. Behav. 55, 1373–1385 (1998).CAS 
PubMed 

Google Scholar 
Miller, A. K., Maritz, B., McKay, S., Glaudas, X. & Alexander, G. J. An ambusher’s arsenal: chemical crypsis in the puff adder (Bitis arietans). Proc. R. Soc. B 282, 20152182 (2015).PubMed 
PubMed Central 

Google Scholar 
Maritz, B. & Alexander, G. J. Dwarfs on the move: Spatial ecology of the world’s smallest viper, Bitis schneideri. Copeia 2012, 115–120 (2012).
Google Scholar 
Stirrat, S. C. Seasonal changes in home-range area and habitat use by the agile wallaby (Macropus agilis). Wildl. Res. 30, 593–600 (2003).
Google Scholar 
Ayers, D. Y. & Shine, R. Thermal influences on foraging ability: Body size, posture and cooling rate of an ambush predator, the python Morelia spilota. Funct. Ecol. 11, 342–347 (1997).
Google Scholar 
Pearson, D., Shine, R. & Williams, A. Spatial ecology of a threatened python (Morelia spilota imbricata) and the effects of anthropogenic habitat change. Austral. Ecol. 30, 261–274 (2005).
Google Scholar 
Freeman, A. A study in power and grace: The amethystine python. Wildl. Aust. 53, 27–29 (2016).
Google Scholar 
Silva, I., Crane, M., Suwanwaree, P., Strine, C. & Goode, M. Using dynamic Brownian bridge movement models to identify home range size and movement patterns in king cobras. PLoS ONE 13, e0203449 (2018).PubMed 
PubMed Central 

Google Scholar 
Marshall, B. M. et al. Space fit for a king: Spatial ecology of king cobras (Ophiophagus hannah) in Sakaerat Biosphere Reserve, Northeastern Thailand. Amphibia-Reptilia 40, 163–178 (2019).
Google Scholar 
Udyawer, V., Simpfendorfer, C. A., Heupel, M. R. & Clark, T. D. Temporal and spatial activity-associated energy partitioning in free-swimming sea snakes. Funct. Ecol. 31, 1739–1749 (2017).
Google Scholar 
Smaniotto, N. P., Moreira, L. F., Rivas, J. A. & Strüssmann, C. Home range size, movement, and habitat use of yellow anacondas (Eunectes notaeus). Salamandra 56, 159–167 (2020).
Google Scholar 
Low, M. R. Rescue, rehabilitation and release of reticulated pythons in Singapore. in Global Reintroduction Perspectives: 2018. Case Studies from Around the Globe (ed. Soorae, P. S.) 78–81 (IUCN/SSC Reintroduction Specialist Group, 2018).Alexander, G. J. & Maritz, B. Sampling interval affects the estimation of movement parameters in four species of African snakes. J. Zool. 297, 309–318 (2015).
Google Scholar 
Smith, B. J. et al. Betrayal: Radio-tagged Burmese pythons reveal locations of conspecifics in Everglades National Park. Biol. Invasions 18, 3239–3250 (2016).
Google Scholar  More

Katsanevakis, S. et al. Impacts of invasive alien marine species on ecosystem services and biodiversity: A pan-European review. Aquat. Invasions 9, 391–423 (2014).
Google Scholar 
Huxel, G. R. Rapid displacement of native species by invasive species: Effects of hybridization. Biol. Conserv. 89, 143–152 (1999).
Google Scholar 
Molnar, J. L., Gamboa, R. L., Revenga, C. & Spalding, M. D. Assessing the global threat of invasive species to marine biodiversity. Front. Ecol. Environ. 6, 485–492 (2008).
Google Scholar 
Blackburn, T. M. et al. A proposed unified framework for biological invasions. Trends Ecol. Evol. 26, 333–339 (2011).PubMed 

Google Scholar 
Ferreira, C. E. L., Gonçalves, J. E. A. & Coutinho, R. Ship hulls and oil platforms as potential vectors to marine species introduction. J. Coast. Res. SI 39 (Pro), 1341–1346 (2006).
Google Scholar 
Glasby, T. M., Connell, S. D., Holloway, M. G. & Hewitt, C. L. Nonindigenous biota on artificial structures: Could habitat creation facilitate biological invasions?. Mar. Biol. 151, 887–895 (2007).
Google Scholar 
Hedge, L. H. & Johnston, E. L. Propagule pressure determines recruitment from a commercial shipping pier. Biofouling 28, 73–85 (2012).PubMed 

Google Scholar 
Capel, K. C. C., Creed, J., Kitahara, M. V., Chen, C. A. & Zilberberg, C. Multiple introductions and secondary dispersion of Tubastraea spp. in the Southwestern Atlantic. Sci. Rep. 9, 1–11 (2019).CAS 

Google Scholar 
De Paula, A. F. & Creed, J. C. Two species of the coral Tubastraea (Cnidaria, Scleractinia) in Brazil: A case of accidental introduction. Bull. Mar. Sci. 74, 175–183 (2004).
Google Scholar 
Lages, B. G., Fleury, B. G., Menegola, C. & Creed, J. C. Change in tropical rocky shore communities due to an alien coral invasion. Mar. Ecol. Prog. Ser. 438, 85–96 (2011).ADS 

Google Scholar 
Mantelatto, M. C., Creed, J. C., Mourão, G. G., Migotto, A. E. & Lindner, A. Range expansion of the invasive corals Tubastraea coccinea and Tubastraea tagusensis in the Southwest Atlantic. Coral Reefs 30, 397–397 (2011).ADS 

Google Scholar 
do Santos, L. A. H., Ribeiro, F. V. & Creed, J. C. Antagonism between invasive pest corals Tubastraea spp. and the native reef-builder Mussismilia hispida in the southwest Atlantic. J. Exp. Mar. Biol. Ecol. 449, 69–76 (2013).
Google Scholar 
Miranda, R. J., Cruz, I. C. S. & Barros, F. Effects of the alien coral Tubastraea tagusensis on native coral assemblages in a southwestern Atlantic coral reef. Mar. Biol. 163, 1–12 (2016).CAS 

Google Scholar 
Silva, A. G., Lima, R. P., Gomes, A. N., Fleury, B. G. & Creed, J. C. Expansion of the invasive corals Tubastraea coccinea and Tubastraea tagusensis into the tamoios ecological station marine protected area, Brazil. Aquat. Invasions 6, S105–S110 (2011).
Google Scholar 
Mizrahi, D., Navarrete, S. A. & Flores, A. A. V. Groups travel further: Pelagic metamorphosis and polyp clustering allow higher dispersal potential in sun coral propagules. Coral Reefs 33, 443–448 (2014).ADS 

Google Scholar 
De Paula, A. F., De Oliveira Pires, D. & Creed, J. C. Reproductive strategies of two invasive sun corals (Tubastraea spp.) in the southwestern Atlantic. J. Mar. Biol. Assoc. UK 94, 481–492 (2014).
Google Scholar 
Capel, K. C. C. et al. Clone wars: Asexual reproduction dominates in the invasive range of Tubastraea spp. (Anthozoa: Scleractinia) in the South-Atlantic Ocean. PeerJ 2017, 1–21 (2017).
Google Scholar 
Luz, B. L. P., Di Domenico, M., Migotto, A. E. & Kitahara, M. V. Life-history traits of Tubastraea coccinea: Reproduction, development, and larval competence. Ecol. Evol. 10, 6223–6238 (2020).PubMed 
PubMed Central 

Google Scholar 
Kitahara, M. V. Species richness and distribution of azooxanthellate scleractinia in Brazil. Bull. Mar. Sci. 81, 497–518 (2007).
Google Scholar 
da Silva, A. G., de Paula, A. F., Fleury, B. G. & Creed, J. C. Eleven years of range expansion of two invasive corals (Tubastraea coccinea and Tubastraea tagusensis) through the southwest Atlantic (Brazil). Estuar. Coast. Shelf Sci. 141, 9–16 (2014).ADS 

Google Scholar 
Creed, J. C. et al. The invasion of the azooxanthellate coral Tubastraea (Scleractinia: Dendrophylliidae) throughout the world: History, pathways and vectors. Biol. Invasions 19, 283–305 (2017).
Google Scholar 
Mantelatto, M. C., Pires, L. M., de Oliveira, G. J. G. & Creed, J. C. A test of the efficacy of wrapping to manage the invasive corals Tubastraea tagusensis and T. coccinea. Manag. Biol. Invasions 6, 367–374 (2015).
Google Scholar 
Crivellaro, M. S. et al. Fighting on the edge: Reproductive effort and population structure of the invasive coral Tubastraea coccinea in its southern Atlantic limit of distribution following control activities. Biol. Invasions 23, 811–823 (2021).
Google Scholar 
Creed, J. C., Casares, F. A., Oigman-Pszczol, S. S. & Masi, B. P. Multi-site experiments demonstrate that control of invasive corals (Tubastraea spp.) by manual removal is effective. Ocean Coast. Manag. 207, 105616 (2021).
Google Scholar 
Sammarco, P. W., Atchison, A. D., Boland, G. S., Sinclair, J. & Lirette, A. Geographic expansion of hermatypic and ahermatypic corals in the Gulf of Mexico, and implications for dispersal and recruitment. J. Exp. Mar. Biol. Ecol. 436–437, 36–49 (2012).
Google Scholar 
Sammarco, P. W., Atchison, A. D. & Boland, G. S. Coral settlement on oil/gas platforms in the northern Gulf of Mexico: Preliminary evidence of rarity. Gulf Mex. Sci. 32, 11–23 (2014).
Google Scholar 
López, C. et al. Invasive Tubastraea spp. and Oculina patagonica and other introduced scleractinians corals in the Santa Cruz de Tenerife (Canary Islands) harbor: Ecology and potential risks. Reg. Stud. Mar. Sci. 29, 100713 (2019).
Google Scholar 
Yeo, D. C. J. et al. Semisubmersible oil platforms: Understudied and potentially major vectors of biofouling-mediated invasions. Biofouling 26, 179–186 (2009).
Google Scholar 
Lockwood, J. L., Cassey, P. & Blackburn, T. M. The more you introduce the more you get: The role of colonization pressure and propagule pressure in invasion ecology. Divers. Distrib. 15, 904–910 (2009).
Google Scholar 
Sammarco, P. W., Atchison, A. D. & Boland, G. S. Expansion of coral communities within the Northern Gulf of Mexico via offshore oil and gas platforms. Mar. Ecol. Prog. Ser. 280, 129–143 (2004).ADS 

Google Scholar 
Macreadie, P. I., Fowler, A. M. & Booth, D. J. Rigs-to-reefs: Will the deep sea benefit from artificial habitat?. Front. Ecol. Environ. 9, 455–461 (2011).
Google Scholar 
Bowler, D. E. & Benton, T. G. Causes and consequences of animal dispersal strategies. Biol. Rev. 80, 205–225 (2005).PubMed 

Google Scholar 
Cowen, R. K. & Sponaugle, S. Larval dispersal and marine population connectivity. Ann. Rev. Mar. Sci. 1, 443–466 (2009).PubMed 

Google Scholar 
Peterson, R. G. & Stramma, L. Upper-level circulation in the South Atlantic Ocean. Prog. Oceanogr. 26, 1–73 (1991).ADS 

Google Scholar 
Johns, W. E. et al. Annual cycle and variability of the North Brazil current. J. Phys. Oceanogr. 28, 103–128 (1998).ADS 

Google Scholar 
Silveira, I. C. A. et al. Brazil current off the eastern Brazilian coast. Rev. Brasil. Oceanog. 48, 171–183 (2000).
Google Scholar 
Soutelino, R. G., Gangopadhyay, A. & da Silveira, I. C. A. The roles of vertical shear and topography on the eddy formation near the site of origin of the Brazil Current. Cont. Shelf Res. 70, 46–60 (2013).ADS 

Google Scholar 
D’Agostini, A., Gherardi, D. F. M. & Pezzi, L. P. Connectivity of marine protected areas and its relation with total kinetic energy. PLoS ONE 10, 1–19 (2015).
Google Scholar 
Endo, C. A. K., Gherardi, D. F. M., Pezzi, L. P. & Lima, L. N. Low connectivity compromises the conservation of reef fishes by marine protected areas in the tropical South Atlantic. Sci. Rep. 9, 1–11 (2019).
Google Scholar 
Hanski, I. Metapopulation dynamics. Nature 396, 41–49 (1998).ADS 
CAS 

Google Scholar 
López-Duarte, P. C. et al. What controls connectivity? An empirical, multi-species approach. Integr. Comp. Biol. 52, 511–524 (2012).PubMed 

Google Scholar 
Batista, D. et al. Distribution of the invasive orange cup coral tubastraea coccinea lesson, 1829 in an upwelling area in the South Atlantic Ocean fifteen years after its first record. Aquat. Invasions 12, 23–32 (2017).
Google Scholar 
O’Connor, M. I. et al. Temperature control of larval dispersal and the implications for marine ecology, evolution, and conservation. Proc. Natl. Acad. Sci. USA. 104, 1266–1271 (2007).ADS 
PubMed 
PubMed Central 

Google Scholar 
Cairns, S. Studies on the natural history of the Caribbean region. Stud. Fauna Curaçao other Caribb. … IXl, (2000).De Paula, A. F. & Creed, J. C. Spatial distribution and abundance of nonindigenous coral genus Tubastraea (Cnidaria, Scleractinia) around Ilha Grande, Brazil. Braz. J. Biol. 65, 661–673 (2005).CAS 
PubMed 

Google Scholar 
Papacostas, K. J. et al. Biological mechanisms of marine invasions. Mar. Ecol. Prog. Ser. 565, 251–268 (2017).ADS 

Google Scholar 
Loureiro, T. G., Silva Gentil Anastácio, P. M., Souty-Grosset, C., Araujo, P. B. & Pereira Almerão, M. Red swamp crayfish: Biology, ecology and invasion—an overview. Nauplius 23, 1–19 (2015).
Google Scholar 
Shanks, A. L., Grantham, B. A. & Carr, M. H. Propagule dispersal distance and the size and spacing of marine reserves. Ecol. Appl. 13, 159–169 (2003).
Google Scholar 
Siegel, D. A. et al. The stochastic nature of larval connectivity among nearshore marine populations. Proc. Natl. Acad. Sci. USA. 105, 8974–8979 (2008).ADS 
CAS 
PubMed 
PubMed Central 

Google Scholar 
Viard, F., Ellien, C. & Dupont, L. Dispersal ability and invasion success of Crepidula fornicata in a single gulf: Insights from genetic markers and larval-dispersal model. Helgol. Mar. Res. 60, 144–152 (2006).ADS 

Google Scholar 
Rodrigues, R. R., Rothstein, L. M. & Wimbush, M. Seasonal variability of the South Equatorial Current bifurcation in the Atlantic Ocean: A numerical study. J. Phys. Oceanogr. 37, 16–30 (2007).ADS 

Google Scholar 
Fenner, D. Biogeography of three Caribbean corals (Scleractinia) and the invasion of Tubastraea coccinea into the Gulf of Mexico. Bull. Mar. Sci. 69, 1175–1189 (2001).
Google Scholar 
Gouveia, M. B. et al. Persistent meanders and eddies lead to quasi-steady Lagrangian transport patterns in a weak western boundary current. Sci. Rep. 11, 1–18 (2021).
Google Scholar 
Campos, E. J., Gonçalves, J. & Ikeda, Y. Water mass characteristics and geostrophic circulation in the South Brazil bight: Summer of 1991. J. Geophys. Res. Oceans 100, 18537–18550. https://doi.org/10.1029/95jc01724 (1995).ADS 
Article 

Google Scholar 
Silveira, I. C. A. et al. Is the meander growth in the Brazil Current system off Southeast Brazil due to baroclinic instability?. Dyn. Atmos. Ocean. 45, 187–207 (2008).ADS 

Google Scholar 
Lima, L. S. et al. Potential changes in the connectivity of marine protected areas driven by extreme ocean warming. Sci. Rep. 11, 1–12 (2021).
Google Scholar 
Thompson, D. M. et al. Variability in oceanographic barriers to coral larval dispersal: Do currents shape biodiversity?. Progr. Oceanogr. 165, 110–122 (2018).ADS 

Google Scholar 
Ellien, C., Thiébaut, E., Dumas, F., Salomon, J. C. & Nival, P. A modelling study of the respective role of hydrodynamic processes and larval mortality on larval dispersal and recruitment of benthic invertebrates: Example of Pectinaria koreni (Annelida: Polychaeta) in the Bay of Seine (English Channel). J. Plankton Res. 26, 117–132 (2004).
Google Scholar 
Leão, Z. M. A. N., Kikuchi, R. K. P. & Testa, V. Corals and coral reefs of Brazil. In Latin American Coral Reefs (ed. Cortés, J.) 9–52 (Elsevier Science, 2003).
Google Scholar 
Dutra, G. F., Allen, G. R., Werner, T., et al. A rapid marine biodiversity assessment of the Abrolhos Bank, Bahia, Brazil. In RAP Bull. Mar. Biol. Assessment, Vol. 38 (Conservation International, 2005).Costa, T. J. F. et al. Expansion of an invasive coral species over Abrolhos Bank, Southwestern Atlantic. Mar. Pollut. Bull. 85, 252–253 (2014).CAS 
PubMed 

Google Scholar 
Moura, R. L. et al. An extensive reef system at the Amazon River mouth. Sci. Adv. 2, 1–12 (2016).
Google Scholar 
Soares, M. O., Davis, M. & de Macêdo Carneiro, P. B. Northward range expansion of the invasive coral (Tubastraea tagusensis) in the southwestern Atlantic. Mar. Biodivers. 48, 1651–1654 (2018).
Google Scholar 
Rocha, L. A. & Rosa, I. L. Baseline assessment of reef fish assemblages of Parcel Manuel Luiz Marine State Park, Maranhão, north-east Brazil. J. Fish Biol. 58, 985–998 (2001).
Google Scholar 
Luz, B. L. P. & Kitahara, M. V. Could the invasive scleractinians Tubastraea coccinea and T. tagusensis replace the dominant zoantharian Palythoa caribaeorum in the Brazilian subtidal?. Coral Reefs 36, 875 (2017).ADS 

Google Scholar 
Cordeiro, C. A. M. M. et al. Conservation status of the southernmost reef of the Amazon Reef System: The Parcel de Manuel Luís. Coral Reefs 40, 165–185 (2021).
Google Scholar 
Rocha, L. A. Patterns of distribution and processes of speciation in Brazilian reef fishes. J. Biogeogr. 30, 1161–1171 (2003).
Google Scholar 
Cruz, R. et al. Life cycle and connectivity of the spiny lobster, Panulirus spp.: Case studies from Brazil and the Wider Caribbean (Decapoda, Achelata). Crustaceana 94, 603–645 (2021).
Google Scholar 
Castro, B. D., Lorenzzetti, J., Silveira, I. D. & Miranda, L. D. Estrutura termohalina e circulação na região entre o cabo de são tomé (rj) eo chuí (rs). O ambiente oceanográfco da plataforma continental e do talude na região sudeste-sul do Brasil 1, 11–120 (2006).
Google Scholar 
Dias, D. F., Pezzi, L. P., Gherardi, D. F. M. & Camargo, R. Modeling the spawning strategies and larval survival of the Brazilian sardine (Sardinella brasiliensis). Prog. Oceanogr. 123, 38–53 (2014).ADS 

Google Scholar 
Nickols, K. J., Wilson White, J., Largier, J. L. & Gaylord, B. Marine population connectivity: Reconciling large-scale dispersal and high self-retention. Am. Nat. 185, 196–211 (2015).PubMed 

Google Scholar 
Vinagre, C. et al. Food web organization following the invasion of habitat-modifying Tubastraea spp. corals appears to favour the invasive borer bivalve Leiosolenus aristatus. Ecol. Indic. 85, 1204–1209 (2018).
Google Scholar 
Capel, K. C. C., Creed, J. C. & Kitahara, M. V. Invasive corals trigger seascape changes in the southwestern Atlantic. Bull. Mar. Sci. 96, 217–218 (2020).
Google Scholar 
Silva, R. et al. Sun coral invasion of shallow rocky reefs: Effects on mobile invertebrate assemblages in Southeastern Brazil. Biol. Invasions 21, 1339–1350 (2019).
Google Scholar 
Creed, J. C. & De Paula, A. F. Substratum preference during recruitment of two invasive alien corals onto shallow-subtidal tropical rocky shores. Mar. Ecol. Prog. Ser. 330, 101–111 (2007).ADS 

Google Scholar 
Glynn, P. W. et al. Reproductive ecology of the azooxanthellate coral Tubastraea coccinea in the Equatorial Eastern Pacific: Part V. Dendrophylliidae. Mar. Biol. 153, 529–544 (2008).
Google Scholar 
Eckman, J. E. Closing the larval loop: Linking larval ecology to the population dynamics of marine benthic invertebrates. J. Exp. Mar. Biol. Ecol. 200, 207–237 (1996).
Google Scholar 
Cairns, S. D. & Zibrowius, H. Azooxanthellate Scleractinia from the Philippines and Indonesian regions. Mémoires du Muséum national d’Histoire naturelle, Vol. 172, (1997).Saura, S., Bodin, Ö. & Fortin, M. J. EDITOR’S CHOICE: Stepping stones are crucial for species’ long-distance dispersal and range expansion through habitat networks. J. Appl. Ecol. 51, 171–182 (2014).
Google Scholar 
Faria, L. C. & Kitahara, M. V. Invasive corals hitchhiking in the Southwestern Atlantic. Ecology 101, 1–3 (2020).
Google Scholar 
Mantelatto, M. C., Póvoa, A. A., Skinner, L. F., de Araujo, F. V. & Creed, J. C. Marine litter and wood debris as habitat and vector for the range expansion of invasive corals (Tubastraea spp.). Mar. Pollut. Bull. 160, 111659 (2020).CAS 
PubMed 

Google Scholar 
Braga, M. D. A. et al. Retirement risks: Invasive coral on old oil platform on the Brazilian equatorial continental shelf. Mar. Pollut. Bull. 165, 112156 (2021).CAS 
PubMed 

Google Scholar 
IMO. Anti-fouling systems. Online (2019). https://www.imo.org/en/OurWork/Environment/Pages/Anti-fouling.aspx. (Accessed 01 May 2021).Vander Zanden, M. J., Hansen, G. J. A., Higgins, S. N. & Kornis, M. S. A pound of prevention, plus a pound of cure: Early detection and eradication of invasive species in the Laurentian Great Lakes. J. Great Lakes Res. 36, 199–205 (2010).
Google Scholar 
Pimentel, D. et al. Economic and environmental threats of alien plant, animal, and microbe invasions. Agric. Ecosyst. Environ. 84(1), 1–20 (2001).
Google Scholar 
Shchepetkin, A. F. & McWilliams, J. C. The regional oceanic modeling system (ROMS): A split-explicit, free-surface, topography-following-coordinate oceanic model. Ocean Model 9, 347–404 (2005).ADS 

Google Scholar 
Shchepetkin, A. F. & McWilliams, J. C. Correction and commentary for “ocean forecasting in terrain-following coordinates: Formulation and skill assessment of the regional ocean modeling system” by haidvogel et al., j. comp. phys. 227, pp. 3595–3624. J. Comput. Phys. 228, 8985–9000 (2009).ADS 
MathSciNet 
MATH 

Google Scholar 
Lett, C. et al. A Lagrangian tool for modelling ichthyoplankton dynamics. Environ. Model. Sofw. 23, 1210–1214 (2008).
Google Scholar 
Gouveia, M. B., Gherardi, D. F. M., Lentini, C. A. D., Dias, D. F. & Campos, P. C. Do the Brazilian sardine commercial landings respond to local ocean circulation?. PLoS ONE 12, 1–19 (2017).
Google Scholar 
Saha, S. et al. The NCEP climate forecast system reanalysis. Bull. Am. Meteorol. Soc. 91, 1015–1057 (2010).ADS 

Google Scholar 
Carton, J. A., Chepurin, G. A. & Chen, L. SODA3: A new ocean climate reanalysis. J. Clim. 31, 6967–6983 (2018).ADS 

Google Scholar 
Flather, R. A. A tidal model of the northeast pacific. Atmos. Ocean 25, 22–45 (1987).
Google Scholar 
Chapman, D. C. Numerical treatment of cross-shelf open boundaries in a barotropic coastal ocean model. J. Phys. Oceanogr. 15(8), 1060–1075 (1985).ADS 

Google Scholar 
Marchesiello, P., McWilliams, J. C. & Shchepetkin, A. Open boundary conditions for long-term integration of regional oceanic models. Ocean Model 3, 1–20 (2001).ADS 

Google Scholar 
Egbert, G. D. & Erofeeva, S. Y. Efficient inverse modeling of barotropic ocean tides. J. Atmos. Ocean. Technol. 19, 183–204 (2002).ADS 

Google Scholar 
Marchesiello, P., McWilliams, J. C. & Shchepetkin, A. Equilibrium structure and dynamics of the California current system. J. Phys. Oceanogr. 33, 753–783 (2003).ADS 

Google Scholar 
Mizrahi, D., Navarrete, S. A. & Flores, A. A. V. Uneven abundance of the invasive sun coral over habitat patches of different orientation: An outcome of larval or later benthic processes?. J. Exp. Mar. Biol. Ecol. 452, 22–30 (2014).
Google Scholar 
Silverman, B. W. Density Estimation for Statistics and Data Analysis (Chapman and Hall, 1986).MATH 

Google Scholar  More

Lázaro-Lobo, A. & Ervin, G. N. A global examination on the differential impacts of roadsides on native versus exotic and weedy plant species. Glob. Ecol. Conserv. 17(e00555), 1–13 (2019).
Google Scholar 
Christen, D. C. & Matlack, G. R. The habitat and conduit functions of roads in the spread of three invasive plant species. Biol. Invasions 11(2), 453–465 (2009).Article 

Google Scholar 
Mortensen, D. A., Rauschert, E. S., Nord, A. N. & Jones, B. P. Forest roads facilitate the spread of invasive plants. Invasive Plant Sci. Manag. 2(3), 191–199 (2009).Article 

Google Scholar 
Lemke, A., Kowarik, I. & von der Lippe, M. How traffic facilitates population expansion of invasive species along roads: The case of common ragweed in Germany. J. Appl. Ecol. 56(2), 413–422 (2019).Article 

Google Scholar 
Rauschert, E. S., Mortensen, D. A. & Bloser, S. M. Human-mediated dispersal via rural road maintenance can move invasive propagules. Biol. Invasions 19(7), 2047–2058 (2017).Article 

Google Scholar 
Meunier, G. & Lavoie, C. Roads as corridors for invasive plant species: New evidence from smooth bedstraw (Galium mollugo). Invasive Plant Sci. Manag. 5(1), 92–100 (2012).Article 

Google Scholar 
Mohit, S., Johnson, T. B. & Arnott, S. E. Recreational watercraft decontamination: Can current recommendations reduce aquatic invasive species spread?. Manag. Biol. Invasions 12(1), 148–164 (2021).Article 

Google Scholar 
Ferguson, L., Duncan, C. L., & Snodgrass, K. Backcountry road maintenance and weed management. United States: U.S. Department of Agriculture, Forest Service, Technology & Development Program. 22pp (2003). At https://www.google.com/books/edition/Backcountry_Road_Maintenance_and_Weed_Ma/y2amRwT1rIsC?hl=en&gbpv=0.Lelong, B., Lavoie, C., Jodoin, C. & Belzile, F. Expansion pathways of the exotic common reed (Phragmites australis): A historical and genetic analysis. Divers. Distrib. 13, 430–437 (2007).Article 

Google Scholar 
Joly, M. et al. Paving the way for invasive species: Road type and the spread of common ragweed (Ambrosia artemisiifolia). Environ. Manag. 48(3), 514–522 (2011).ADS 
Article 

Google Scholar 
Thompson, D. Q., Stuckey, R. L. & Thompson, E. B. Spread, impact, and control of purple loosestrife (Lythrum salicaria) in North American wetlands. U. S. Fish and Wildlife Service (1987). At http://stoppinginvasives.com/dotAsset/670d2f92-cd0c-41ab-9955-7204f1a9a192.pdf.Stuckey, R. L. Distributional history of Lythrum salicaria (purple loosestrife) in North America. Bartonia 47, 3–20 (1980).
Google Scholar 
Blossey, B., Skinner, L. C. & Taylor, J. Impact and management of purple loosestrife (Lythrum salicaria) in North America. Biodivers. Conserv. 10(10), 1787–1807 (2001).Article 

Google Scholar 
Wilcox, D. A. Migration and control of purple loosestrife (Lythrum salicaria L.) along highway corridors. Environ. Manag. 13(3), 365–370 (1989).ADS 
Article 

Google Scholar 
St. Louis, E., Stastny, M. & Sargent, R. D. The impacts of biological control on the performance of Lythrum salicaria 20 years post-release. Biol. Control. 140, 104–123 (2020).Article 

Google Scholar 
NYSDOT Environmental Science Bureau. Environmental Handbook for Transportation Operations: A Summary of the Environmental Requirements and Best Practices for Maintaining the Constructing Highways and Transportation Systems. Prepared by NYSDOT Environmental Science Bureau, (2011) At https://www.dot.ny.gov/divisions/engineering/environmental-analysis/repository/oprhbook.pdf.Blossey, B., Schroeder, D., Hight, S. D. & Malecki, R. A. Host specificity and environmental impact of two leaf beetles (Galerucella calmariensis and G. pusilla) for biological control of purple loosestrife (Lythrum salicaria). Weed Sci. 42, 134–140 (1994).Article 

Google Scholar 
Blossey, B. Before, during and after: The need for long-term monitoring in invasive plant species management. Biol. Invasions 1, 301–311 (1999).Article 

Google Scholar 
Blossey, B. & Hunt, T. R. Mass rearing methods for Galerucella calmariensis and G. pusilla (Coleoptera: Chrysomelidae), biological control agents of Lythrum salicaria (Lythraceae). J. Econ. Entomol. 92(2), 325–334 (1999).CAS 
Article 

Google Scholar 
Grevstad, F. S. Ten-year impacts of the biological control agents Galerucella pusilla and G. calmariensis (Coleoptera: Chrysomelidae) on purple loosestrife (Lythrum salicaria) in Central New York State. Biol. Control 39(1), 1–8 (2006).Article 

Google Scholar 
Boag, A. E. & Eckert, C. G. The effect of host abundance on the distribution and impact of biocontrol agents on purple loosestrife (Lythrum salicaria, Lythraceae). Écoscience 20(1), 90–99 (2013).Article 

Google Scholar 
Lakoba, V. T., Brooks, R. K., Haak, D. C. & Barney, J. N. An Analysis of US State regulated weed lists: A discordance between biology and policy. Bioscience 70(9), 804–813 (2020).Article 

Google Scholar 
Welling, C. H. & Becker, R. L. Seed bank dynamics of Lythrum salicaria L.: Implications for control of this species in North America. Aquat. Bot. 38, 303–309 (1990).Article 

Google Scholar 
Brown, B. J. & Wickstrom, C. E. Adventitious root production and survival of purple loosestrife (Lythrum salicaria) shoot sections. Ohio J. Sci. 97, 2–4 (1997).
Google Scholar 
Farnsworth, E. J. & Ellis, D. R. Is purple loosestrife (Lythrum salicaria) an invasive threat to freshwater wetlands? Conflicting evidence from several ecological metrics. Wetlands 21(2), 199–209 (2001).Article 

Google Scholar 
Mahaney, W. M., Smemo, K. A. & Yavitt, J. B. Impacts of Lythrum salicaria invasion on plant community and soil properties in two wetlands in central New York, USA. Botany 84(3), 477–484 (2006).
Google Scholar 
Treberg, M. A. & Husband, B. C. Relationship between the abundance of Lythrum salicaria (purple loosestrife) and plant species richness along the Bar River Canada. Wetlands 19(1), 118–125 (1999).Article 

Google Scholar 
Hager, H. & Vinebrooke, R. E. Positive relationships between invasive purple loosestrife (Lythrum salicaria) and plant species diversity and abundance in Minnesota wetlands. Can. J. Bot. 82(6), 763–773 (2004).Article 

Google Scholar 
Lavoie, C. Should we care about purple loosestrife? The history of an invasive plant in North America. Biol. Invasions 12(7), 1967–1999 (2010).Article 

Google Scholar 
Fickbohm, S. S. & Zhu, W. X. Exotic purple loosestrife invasion of native cattail freshwater wetlands: Effects on organic matter distribution and soil nitrogen cycling. Appl. Soil. Ecol. 32(1), 123–131 (2006).Article 

Google Scholar 
Ramula, S. Annual mowing has the potential to reduce the invasion of herbaceous Lupinus polyphyllus. Biol. Invasions 22(10), 3163–3173 (2020).Article 

Google Scholar 
Milakovic, I., Fiedler, K. & Karrer, G. Management of roadside populations of invasive Ambrosia artemisiifolia by mowing. Weed Res. 54(3), 256–264 (2014).Article 

Google Scholar 
Vitalos, M. & Karrer, G. Dispersal of Ambrosia artemisiifolia seeds along roads: The contribution of traffic and mowing machines. Neobiota 8, 53–60 (2009).
Google Scholar 
Forman, R. T. & Alexander, L. E. Roads and their major ecological effects. Annu. Rev. Ecol. Syst. 29(1), 207–231 (1998).Article 

Google Scholar 
Milt, A. W. et al. Minimizing opportunity costs to aquatic connectivity restoration while controlling an invasive species. Conserv. Biol. 32(4), 894–904 (2018).Article 

Google Scholar 
RStudio Team. RStudio: Integrated Development Environment for R. RStudio, PBC. (2021). URL http://www.rstudio.com/.R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing. (2021). https://www.R-project.org/.U. S. Fish and Wildlife Service. National Wetlands Inventory. http://www.fws.gov/wetlands/ (2020).Yakimowski, S. B., Hager, H. A. & Eckert, C. G. Limits and effects of invasion by the nonindigenous wetland plant Lythrum salicaria (purple loosestrife): A seed bank analysis. Biol. Invasions 7, 687–698 (2005).Article 

Google Scholar 
Thomas, S. M. & Moloney, K. A. Combining the effects of surrounding land-use and propagule pressure to predict the distribution of an invasive plant. Biol. Invasions 17, 477–495 (2015).Article 

Google Scholar 
Barbier, E. B., Knowler, D., Gwatipedza, J., Reichard, S. H. & Hodges, A. R. Implementing policies to control invasive plant species. Bioscience 63(2), 132–138 (2013).Article 

Google Scholar 
Blossey, B. Measuring and Evaluating Ecological Outcomes of Biological Control Introductions. In Integrating Biological Control into Conservation Practice (eds Van Driesche, R. et al.) 161–188 (Wiley, 2016).Chapter 

Google Scholar 
Rowell, N. Warren County Purple Loosestrife Management Program Final Report. (2015). At https://www.warrenswcd.org/reports.html.Vanneste, T. et al. Plant diversity in hedgerows and road verges across Europe. J. Appl. Ecol. 57(7), 1244–1257 (2020).Article 

Google Scholar 
Auffret, A. G. & Lindgren, E. Roadside diversity in relation to age and surrounding source habitat: Evidence for long time lags in valuable green infrastructure. Ecol. Solut. Evid. 1(1), e12005 (2020).Article 

Google Scholar 
Mccleery, R. A., Holdorf, A. R., Hubbard, L. L. & Peer, B. D. Maximizing the wildlife conservation value of road right-of-ways in an agriculturally dominated lands. Plos one 10(3), e0120375 (2015).Article 

Google Scholar 
New York Invasive Species Information (NYISI). Purple Loosestrife. (2019). at http://nyis.info/invasive_species/purple-loosestrife.Rogers, J. Controlling purple loosestrife (Lythrum Salicaria) along roadsides in St. Lawrence County: Monitoring and biological controls. Adirondack J. Environ. Stud. 23(1), 5 (2019).
Google Scholar 
New York State Department of Transportation. Clear Zones. (2021). At https://www.dot.ny.gov/divisions/engineering/environmental-analysis/landscape/trees/rs-lsf-plant-photos.ESRI. ArcGIS Pro: Version 2.9: Environmental System Research Institute. (2021). At https://pro.arcgis.com/en/pro-app/latest/get-started/get-started.htm.IBM Corp. IBM SPSS Statistics for Windows, Version 25.0. Armonk, NY: IBM Corp. Released 2017. More