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    Evaluating the effects of giraffe skin disease and wire snare wounds on the gaits of free-ranging Nubian giraffe

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    Life history strategies among soil bacteria—dichotomy for few, continuum for many

    Data were analyzed from samples collected, processed, and published previously [21, 25, 29] and have been summarized here. The present analysis, which consisted of sequence data processing, the calculation of taxon-specific isotopic signatures, and subsequent analyses, reflects original work.Sample collection and isotope incubationTo generate experimental data, three replicate soil samples were collected from the top 10 cm of plant-free patches in four ecosystems along the C. Hart Merriam elevation gradient in Northern Arizona. From low to high elevation, these sites are located in the following environments: desert grassland (GL; 1760 m), piñon-pine juniper woodland (PJ; 2020 m), ponderosa pine forest (PP; 2344 m), and mixed conifer forest (MC; 2620 m). Soil samples were air-dried for 24 h at room temperature, homogenized, and passed through a 2 mm sieve before being stored at 4 °C for another 24 h. This produced three distinct but homogenous soil samples from each of the four ecosystems that were subject to experimental treatments. Three treatments were applied to bring soils to 70% water-holding capacity: water alone (control), water with glucose (C treatment; 1000 µg C g−1 dry soil), or water with glucose and a nitrogen source (CN treatment; [NH4]2SO4 at 100 µg N g−1 dry soil). To track growth through isotope assimilation, both 18O-enriched water (97 atom %) and 13C-enriched glucose (99 atom %) were used. In all treatments isotopically heavy samples were paired with matching “light” samples that received water with a natural abundance isotope signatures. For 18O incubations, this design resulted in three soil samples per ecosystem per treatment (across four ecosystems and three treatments, n = 36) while 13C incubations were limited to only C and CN treatments (n = 24). Previous analyses suggest that three replicates is sufficient to detect growth of 10 atom % 18O in microbial DNA with a power of 0.6 and a growth of 5 atom % 18O with a power of 0.3 (12 and 6 atom % respectively for 13C) [30]. All soils were incubated in the dark for one week. Following incubation, soils were frozen at −80 °C for one week prior to DNA extraction.Quantitative stable isotope probingThe procedure of qSIP (quantitative stable isotope probing) is described here but has been applied to these samples as previously published [17, 21, 25]. DNA extraction was performed on soils using a DNeasy PowerSoil HTP 96 Kit (MoBio Laboratories, Carlsbad, CA, USA) and following manufacturer’s protocol. Briefly, 0.25 g of soils from each sample were carefully added to deep, 96-well plates containing zirconium dioxide beads and a cell lysis solution with sodium dodecyl sulfate (SDS) and shaken for 20 min. Following cell lysis, supernatant was collected and centrifuged three times in fresh 96-well plates with reagents separating DNA from non-DNA organic and inorganic materials. Lastly, DNA samples were collected on silica filter plates, rinsed with ethanol and eluted into 100 µL of a 10 mM Tris buffer in clean 96-well plates. To quantify the degree of 18O or 13C isotope incorporation into bacterial DNA (excess atom fraction or EAF), the qSIP protocol [31] was used, though modified slightly as reported previously [21, 24, 32]. Briefly, microbial growth was quantified as the change in DNA buoyant density due to incorporation of the 18O or 13C isotopes through the method of density fractionation by adding 1 µg of DNA to 2.6 mL of saturated CsCl solution in combination with a gradient buffer (200 mM Tris, 200 mM KCL, 2 mM EDTA) in a 3.3 mL OptiSeal ultracentrifuge tube (Beckman Coulter, Fullerton, CA, USA). The solution was centrifuged to produce a gradient of increasingly labeled (heavier) DNA in an Optima Max bench top ultracentrifuge (Beckman Coulter, Brea, CA, USA) with a Beckman TLN-100 rotor (127,000 × g for 72 h) at 18 °C. Each post-incubation sample was thus converted from a continuous gradient into approximately 20 fractions (150 µL) using a modified fraction recovery system (Beckman Coulter). The density of each fraction was measured with a Reichart AR200 digital refractometer (Reichert Analytical Instruments, Depew, NY, USA). Fractions with densities between 1.640 and 1.735 g cm−3 were retained as densities outside this range generally did not contain DNA. In all retained fractions, DNA was cleaned and purified using isopropanol precipitation and the abundance of bacterial 16S rRNA gene copies was quantified with qPCR using primers specific to bacterial 16S rRNA genes (Eub 515F: AAT GAT ACG GCG ACC ACC GAG TGC CAG CMG CCG CGG TAA, 806R: CAA GCA GAA GAC GGC ATA CGA GGA CTA CVS GGG TAT CTA AT). Triplicate reactions were 8 µL consisting of 0.2 mM of each primer, 0.01 U µL−1 Phusion HotStart II Polymerase (Thermo Fisher Scientific, Waltham, MA), 1× Phusion HF buffer (Thermo Fisher Scientific), 3.0 mM MgCl2, 6% glycerol, and 200 µL of dNTPs. Reactions were performed on a CFX384 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) under the following cycling conditions: 95 °C at 1 min and 44 cycles at 95 °C (30 s), 64.5 °C (30 s), and 72 °C (1 min). Separate from qPCR, retained sample-fractions were subject to a similar amplification step of the 16S rRNA gene V4 region (515F: GTG YCA GCM GCC GCG GTA A, 806R: GGA CTA CNV GGG TWT CTA AT) in preparation for sequencing with the same reaction mix but differing cycle conditions – 95 °C for 2 min followed by 15 cycles at 95 °C (30 s), 55 °C (30 s), and 60 °C (4 min). The resulting 16S rRNA gene V4 amplicons were sequenced on a MiSeq sequencing platform (Illumina, Inc., San Diego, CA, USA). DNA sequence data and sample metadata have been deposited in the NCBI Sequence Read Archive under the project ID PRJNA521534.Sequence processing and qSIP analysisIndependently from previous publications, we processed raw sequence data of forward and reverse reads (FASTQ) within the QIIME2 environment [33] (release 2018.6) and denoised sequences within QIIME2 using the DADA2 pipeline [34]. We clustered the remaining sequences into amplicon sequence variants (ASVs, at 100% sequence identity) against the SILVA 138 database [35] using a pre-trained open-reference Naïve Bayes feature classifier [36]. We removed samples with less than 3000 sequence reads, non-bacterial lineages, and global singletons and doubletons. We converted ASV sequencing abundances in each fraction to the number of 16S rRNA gene copies per gram dry soil based on qPCR abundances and the known amount of dry soil equivalent added to the initial extraction. This allowed us to express absolute population densities, rather than relative abundances. Across all replicates, we identified 114 543 unique bacterial ASVs.We calculated the 18O and 13C excess atom fraction (EAF) for each bacterial ASV using R version 4.0.3 [37] and data.table [38] with custom scripts available at https://www.github.com/bramstone/. Negative enrichment values were corrected using previously published methods [17]. ASVs that appeared in less than two of the three replicates of an ecosystem-treatment combination (n = 3) and less than three density fractions within those two replicates were removed to avoid assigning spurious estimates of isotope enrichment to infrequent taxa. Any ASVs filtered out of one ecosystem-treatment group were allowed to be present in another if they met the frequency threshold. Applying these filtering criteria, we limited our analysis towards 3759 unique bacterial ASVs which accounted for a small proportion of the total diversity but represented 68.0% of all sequence reads, and encompassed most major bacterial groups (Supplementary Fig. 1).Analysis of life history strategies and nutrient responseAll statistical tests were conducted in R version 4.0.3 [37]. We assessed the ability of phylum-level assignment of life history strategy to predict growth in response to C and N addition, as proxied by the incorporation of heavy isotope during DNA replication [39, 40]. Phylum-level assignments (Table 1) were based on the most frequently observed behavior of lineages with a representative phylum (or subphylum) as compiled previously [23]. We averaged 18O EAF values of bacterial taxa for each treatment and ecosystem and then subtracted the values in control soils from values in C-amended soils to determine C response (∆18O EAFC) and from the 18O EAF of bacteria in CN-amended soils to determine C and N response (Δ18O EAFCN). Because an ASV must have a measurable EAF in both the control and treatment for a valid Δ18O EAF to be calculated, we were only able to resolve the nutrient response for 2044 bacterial ASVs – 1906 in response to C addition and 1427 in response to CN addition.We used Gaussian finite mixture modeling, as implemented by the mclust R package [41], to demarcate plausible multi-isotopic signatures for oligotrophs and copiotrophs. For each treatment, we calculated average per-taxon 13C and 18O EAF values. To compare both isotopes directly, we divided 18O EAF values by 0.6 based on the estimate that this value (designated as µ) represents the fraction of oxygen atoms in DNA derived from the 18O-water, rather than from 16O within available C sources [42]. Two mixture components, corresponding to oligotrophic and copiotrophic growth modes, were defined using the Mclust function using ellipsoids of equal volume and shape. We observed several microorganisms with high 18O enrichment but comparatively low 13C enrichment, potentially indicating growth following the depletion of the added glucose, and that were reasonably clustered as oligotrophs in our mixture model.We tested how frequently mixture model clustering of each microorganism’s growth (based on average 18O–13C EAF in a treatment) could predict its growth across replicates (n = 12 in each treatment—although individual). We applied the treatment-level mixture models defined above to the per-taxon isotope values in each replicate, recording when a microorganism’s life history strategy in a replicate agreed with the treatment-level cluster, and when it didn’t. We used exact binomial tests to test whether the number of “successes” (defined as a microorganism being grouped in the same life history category as its treatment-level cluster) was statistically significant. To account for type I error across all individual tests (one per ASV per treatment), we adjusted P values in each treatment using the false-discovery rate (FDR) method [43].To determine the extent that life history categorizations may be appropriately applied at finer levels of taxonomic resolution, we constructed several hierarchical linear models using the lmer function in the nlme package version 3.1-149 [44]. To condense growth information from both isotopes into a single analysis, 18O and 13C EAF values were combined into a single variable using principal components analysis separately for each treatment. Across the C and CN treatments, the first principal component (PC1) was able to explain – respectively – 86% and 91% of joint variation of 18O and 13C EAF values. In all cases, we applied PC1 as the response variable and treated taxonomy and ecosystem as random model terms to limit the potential of pseudo-replication to bias significance values. We used likelihood ratio analysis and Akaike information criterion (AIC) values to compare models where life history strategy was determined based on observed nutrient responses at different taxonomic levels (Eq. 1) against a model with the same random terms but without any life history strategy data (Eq. 2). Separate models were applied to each treatment. To reduce model overfitting, we removed families represented by fewer than three bacterial ASVs as well as phyla represented by only one order. In addition, we removed bacterial ASVs with unknown taxonomic assignments (following Morrissey et al. [21]). This limited our analysis to 1 049 ASVs in the C amendment and 984 in the CN amendment.$${{{{{rm{PC}}}}}}{1}_{{18{{{{{rm{O}}}}}} – 13{{{{{rm{C}}}}}}}}sim {{{{{rm{strategy}}}}}} + 1|{{{{{rm{phylum}}}}}}/{{{{{rm{class}}}}}}/{{{{{rm{order}}}}}}/{{{{{rm{family}}}}}}/{{{{{rm{genus}}}}}}/{{{{{rm{eco}}}}}}$$
    (1)
    $${{{{{rm{PC}}}}}}{1}_{{18{{{{{rm{O}}}}}} – 13{{{{{rm{C}}}}}}}}sim 1 + 1|{{{{{rm{phylum}}}}}}/{{{{{rm{class}}}}}}/{{{{{rm{order}}}}}}/{{{{{rm{family}}}}}}/{{{{{rm{genus}}}}}}/{{{{{rm{eco}}}}}}$$
    (2)
    Here, life history strategy was defined at each taxonomic level using the mixture models above and based on the mean 18O and 13C EAF values of each bacterial lineage (Supplemental Fig. 2). We compared these models with the no-strategy model (Eq. 2) directly using likelihood ratio testing. More

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    Understanding the role of natural and anthropogenic forcings in structuring the periphytic algal assemblages in a regulated river ecosystem

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    A high-resolution gridded grazing dataset of grassland ecosystem on the Qinghai–Tibet Plateau in 1982–2015

    Study areaThe Qinghai–Tibet Plateau (26°00′-39°47′N, 73°19′-104°47′E), one of the most important pastoral areas in the world, straddles the southwest regions of China, and it includes 244 counties, which belong to six provinces: Tibet, Qinghai, Xinjiang, Gansu, Sichuan, and Yunnan. It is characterized by rich natural grassland resources, including desert steppes, alpine steppes, and alpine meadows (Fig. 1a). The grassland areas account for over 56% of this region34. The grassland plays a vital role in providing regional and national animal husbandry products and fodder35, which enables the local herders to obtain almost all of the resources required for survival36. The grazing density distribution is extremely unbalanced (Fig. 1a) owing to the high spatial heterogeneity of economic development (Fig. 1b-1) and grassland production (Fig. 1b-2), resulting from the differences in resources and environmental factors37. Over the past few decades, there has been a significant change in the number of livestock animals, and the number of sheep exceeded 160 million by 2020. Therefore, it is urgent to obtain a high-resolution gridded grazing dataset for its evaluating spatiotemporal changes and coordinating the relationship between human beings and the grassland ecosystem.Fig. 1Location of the Qinghai–Tibet Plateau: (a) grassland type and distribution, and grazing density (GD) in 244 counties; (b) spatial heterogeneity of economic development (ED) and grassland production (GP) in 244 counties. GD, ED, and GP are represented by sheep unit per grassland area per county (SU/hm2), human footprint index per pixel (HF/pixel) per county, and net primary production per grassland area per county (gC/m2), respectively.Full size imageFig. 2Methodological framework for grazing spatialization.Full size imageMethodological frameworkWe developed a methodological framework for high-resolution gridded grazing dataset mapping. The framework mainly includes four parts: (i) identifying features affecting grazing, (ii) extracting theoretical suitable grazing areas, (iii) building grazing spatialization model, and (iv) correcting the grazing spatialization dataset. Each step is explained in more detail below (Fig. 2).Step 1: Identifying features affecting grazingGrazing activities are affected by the spatial heterogeneity of resources and environmental factors, regulated by the grazing behavior of herders and the foraging behavior of herds, and restricted by ecological protection policies. Therefore, the specific implications of the 14 influencing factors from the above four aspects are presented in Table 1. These factors are necessary for spatializing the county-level grazing data.Table 1 The identified features affecting grazing.Full size tableStep 2: Extracting theoretical suitable grazing areasThe decision tree approach38 was adopted to extract the theoretical suitable grazing areas for further grazing spatialization (step 2 in Fig. 2). First, the potential grazing area was identified according to the boundary of the grassland ecosystem, because grazing behavior only occurs in the grassland. Then, the unsuitable areas for grazing, i.e., extremely-high-altitude areas and areas adjacent to towns, were removed from the potential grazing area stepwise. The areas strictly prohibited for grazing, i.e., the core areas of national nature reserves39 within grassland areas, were also deemed unsuitable for grazing. Finally, the extracted areas were the theoretically suitable grazing areas.Step 3: Building grazing spatialization model(i) Extracting cross-scale feature (CSFs)In the traditional method, the spatial resolution of the training data (i.e., the average value at the administrative level) differs from that of the predicting data (i.e., the value at the pixel level), and the trained model can only capture the characteristics within the training data. However, the extreme value of the predicting data inevitably exceeds the range of the training data, which can result in underestimation in these parts40. To reduce these mismatches, we built an improved method for CSFs extraction (Fig. 2, first part of step 3).First, the census grazing data are simply distributed from county level to pixel level using the weight of the absolute disturbance (AD) index as Eq. (1). The AD index is measured by Mahalanobis distance using Eq. (2), which is calculated according to the deviation between the potential and observed normalized difference vegetation index (NDVI) values22. Second, the distributed grazing data are graded via the hierarchical clustering method, and the optimal number of the group can be determined using the Davies–Bouldin index (DBI)41 as Eq. (3), an index for evaluating the quality of clustering algorithm. The smaller the DBI, the smaller the distance within each group. Therefore, the DBI can be used to select the best similar values to minimize the deviation within each group. Finally, we can obtain the scope of the groups within each county using the above two steps and obtain the average value of all independent variables and the dependent variable accordingly. As expected, we can decompose the average value at the county level (traditional features in Fig. 2) into the average value at the group level (improved features in Fig. 2).$$S{U}_{i}=S{U}_{j}^{C}frac{{w}_{A{D}_{i}}}{{w}_{A{D}_{j}}}$$
    (1)
    where SUi and (S{U}_{j}^{C}) are the grazing value for pixel i and the census grazing value for county j; ({w}_{A{D}_{i}}) is the weight of the AD index for pixel i and ({w}_{A{D}_{j}}) represents the summed weight of the AD index values for all pixels in county j.$$begin{array}{cll}A{D}_{i} & = & sqrt{{({D}_{i}-u)}^{T}co{v}^{-1}({D}_{i}-u)}\ {D}_{i} & = & NDV{I}_{i}^{T}-NDV{I}_{i}^{P}end{array}$$
    (2)
    where ADi is the AD index for pixel i; the vector composed of its observed NDVI (left(NDV{I}_{i}^{T}right)) and potential NDVI (left(NDV{I}_{i}^{P}right)) time-series data could be considered as two points in the feature space for pixel i, and Di and u are the difference and the mean value of the vector, respectively; cov is the covariance matrix.$$DB{I}_{k}=frac{1}{k}{sum }_{x=1}^{k}ma{x}_{yne x}left(frac{overline{{a}_{x}}+overline{{a}_{y}}}{left|{delta }_{x}-{delta }_{y}right|}right)$$
    (3)
    where DBIk is the DBI coefficient when the cluster number is k; (overline{{a}_{x}}) and (overline{{a}_{y}}) are the average distances of the group xth and the group yth, respectively; δx and δy are the center distance of the group xth and the group yth, respectively.Different from the traditional method, our method can decompose features into multiple features using the grading AD index. The differences among counties will not be easily averaged out. Moreover, our method is less affected by scale mismatch and can be transferred to cross-scale modeling26.(ii) Building RF model with partitioningA single model cannot accurately obtain the variation information of the Qinghai–Tibet Plateau with high spatial heterogeneity. The partition model, a widely used method for estimating population distribution and others42,43, can be incorporated into the proposed model to improve its performance. The thresholds (0.43, 0.35 and 0.21 SU/hm2), determined according to the theoretical livestock carrying capacity (equation S1), were calculated and used to separate independent variables and dependent variable for each grassland types: alpine meadow, alpine steppe and alpine desert steppe (see Section 6.1 for details). Then, the RF models were established, and the training and testing samples were randomly divided in the proportion of 3:1. It is notable that transforming the response variable using natural log prior to RF model fitting is necessary to achieve higher prediction accuracies44. Finally, the independent variables at the pixel level were inputted into the two trained RF models, and the corresponding grid grazing dataset was output by combining the two results (Fig. 2, second part of step 3).(iii) Validating the accuracy of the methodsThe performance of the grazing spatialization model was evaluated through a comparison of the predicted value with census value26. Accuracy validation indexes, including coefficients of determination (R2), root mean square error (RMSE), and mean absolute error (MAE), were used to evaluate the performances of the proposed RF-based models (Table 2), as presented in Eq. (4).$$begin{array}{ccc}{R}^{2} & = & 1-frac{{sum }_{j=1}^{N}{left(S{U}_{j}^{C}-S{U}_{j}^{P}right)}^{2}}{{sum }_{j=1}^{N}{left(S{U}_{j}^{C}-overline{S{U}^{C}}right)}^{2}}\ RMSE & = & sqrt{frac{{sum }_{j=1}^{N}{left(S{U}_{j}^{C}-S{U}_{j}^{P}right)}^{2}}{N}}\ MAE & = & frac{{sum }_{j=1}^{N}| S{U}_{j}^{C}-S{U}_{j}^{P}| }{N}end{array}$$
    (4)
    where (S{U}_{j}^{C}) and (S{U}_{j}^{P}) are the census grazing value and the predicted grazing value for county j, respectively; (overline{S{U}^{C}}) is the average census data for all counties; and N is the number of all counties.Table 2 The proposed methods and their descriptions.Full size tableStep 4: Correcting grazing spatialization dataset(i) Correcting residuals of datasetCorrecting residuals is necessary to obtain datasets with higher accuracy45,46, because propagating the cross-scale relationship in the RF models will inevitably generate errors47. The residuals, calculated by the difference between the average census grazing and predicted grazing values at the administrative level, were used to calibrate the errors related to all pixels within this county. The revised dataset after residual correction is the final product provided in this study. The residual correction method is expressed by Eq. (5), and the process is shown in the fourth step in Fig. 2.$$S{U}_{i}^{RP}=S{U}_{i}^{P}+{R}_{j}$$
    (5)
    where (S{U}_{i}^{RP}) denotes the predicted grazing value revised by the residuals for pixel i, (S{U}_{i}^{P}) denotes the predicted grazing for pixel i, and Rj denotes the residuals calculated from the difference between census grazing and predicted grazing data for county j.(ii) Validating the accuracy of datasetTwo goodness-of-fit indexes were used to validate the consistency of spatial distribution and the temporal trend between predicted grazing data and census grazing data. Generally, the coefficient of determination (R2), defined in Eq. (4), is used to verify the consistency of spatial distribution, and the Nash–Sutcliffe efficiency (NSE, Eq. (6)) is used to verify the consistency of temporal trend. An index value closer to 1 corresponds to a more accurate dataset. Meanwhile, we also collected field grazing data from 56 sites to further validate the spatial accuracy of the dataset, and it measured using the R2 in Eq. (4).$$NSE=1-frac{{sum }_{t=1}^{T}{left(S{U}_{t}^{RP}-S{U}_{t}^{C}right)}^{2}}{{sum }_{t=1}^{T}{left(S{U}_{t}^{C}-overline{S{U}^{{C}^{{prime} }}}right)}^{2}}$$
    (6)
    where (S{U}_{t}^{RP}) and (S{U}_{t}^{C}) are the predicted grazing value revised by residuals and the census grazing value of all counties in year t, respectively; (overline{S{U}^{{C}^{{prime} }}}) is the average census grazing value of all years; and T is the number of time steps.(iii) Identifying uncertainties associated with datasetThe uncertainties associated with the dataset originate from the following two aspects: First, the unreasonableness of our method, owing to the errors related to cross-scale modeling or the inappropriate selection of influencing factors, is an important source of uncertainties. Second, the incompleteness of auxiliary variables also introduces uncertainties. In this instance, grassland-free areas are not accurately identified in some counties, but livestock animals are raised in these counties. These counties have no effective value for livestock density prediction. Overall, the uncertainties can be identified in terms of the mean relative error (MRE) in Eq. (7).$$MRE=frac{{sum }_{j=1}^{N}left|frac{S{U}_{j}^{C}-S{U}_{j}^{RP}}{S{U}_{j}^{C}}right|}{N}ast 100 % $$
    (7)
    where (S{U}_{j}^{C}) is the census grazing value for county j, (S{U}_{j}^{RP}) is the predicted grazing value revised by residuals for county j, and N is the number of counties.Data sourceCensus grazing data at county levelEight types of livestock, namely cattle, yaks, horses, donkeys, mules, camels, goats, and sheep, were considered according to the regional characteristics, and livestock stocking quantity at the end of year for each county can be determined from statistical yearbooks. However, the numbers of livestock at the county level for some years between 1982 and 2015 were not recorded. The missing data were indirectly approximated from city- or provincial-level data (e.g., interpolation using their temporal trends). Each type of livestock stocking quantity was converted into standard sheep unit (SU) according to the national standards using Eq. (8)48, namely the calculation of rangeland carrying capacity (NY/T 635-2015). Of the 244 counties of the Qinghai–Tibet Plateau, only 242 counties were considered, as the census grazing data for the other 2 counties were unavailable. The unit of grazing statistics data at the county level is defined as SU per county per year (SU·county−1·year−1).$$begin{array}{l}SU={N}_{sheep}+0.8times {N}_{goats}+5times {N}_{cattle}+5times {N}_{yaks+}+\ 6times {N}_{horses}+3times {N}_{donkeys}+6times {N}_{mules}+7times {N}_{camels}end{array}$$
    (8)
    where Nsheep, Ngoats, Ncattle, Nyaks, Nhorses, Ndonkeys, Nmules, Ncamels are the number of sheep, goats, cattle, yaks, horses, donkeys, mules, and camels at the year-end, respectively. SU denotes the standard sheep unit (SU·county−1·year−1).Data of grazing influencing factors at pixel levelThe types of features affecting grazing were obtained from the first step described in Methods, and the detailed information, such as original spatiotemporal resolution, format, and source, is shown in Table 3. The format (i.e., GeoTIFF), spatial resolution (i.e., 0.083°), and the number of rows and columns of the gridded features were leveraged to further produce a high-resolution grazing dataset.Table 3 Data source of grazing influence factors.Full size table More

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    Analysis of Himalayan marmot distribution and plague risk in Qinghai province of China using the “3S” technology

    Himalayan marmot habitat analysisThe environmental factors including temperature, vegetation and elevation are the key drivers for the wildlife in alpine ecosystems32. Specific landform attributes such as slope and elevation and vegetation cover affect the population and burrowing of rodents33. For example, rodent burrows in the Western Usambara Mountains in Tanzania were only found at an elevation of above 1600 m33. However, the Himalayan marmot seems to prefer to inhabit areas with low elevation and high land surface temperature34. In this study, the data showed that 76.25% of the Himalayan marmots were found in areas with elevation values of 3400–4600 m. The majority of marmots were found in areas with slopes of 5–20° and vegetation cover higher than 60%. Most marmots were found in alpine meadows, a few were found in temperate grasslands and alpine grasslands, and none were found in other grassland types.Preliminary statistical analysis of vegetation cover, grass type, vegetation type, and Himalayan marmot distribution sample sites obtained using spatial geographic information technology revealed that the meadow grassland areas with lush grass growth, more dominant plants, and abundant food had more marmots. When the vegetation cover reached 0.60–1.00, the number of marmot distribution sample sites was the highest. Dense grass is an ideal habitat and provides concealment for Himalayan marmots, and the abundant plant types provide sufficient food for marmots. In contrast, no marmots were distributed in the alpine scrub, coniferous forest, and alpine snow/ice covered areas where vegetation growth was poor, vegetation cover was low, and food was relatively scarce. Moreover, 70.24% of Himalayan marmots were found in alpine meadows with a wide variety of plant species, including Poaceae, Cyperaceae, and grasses. This finding indicated that alpine meadows are more suitable for Himalayan marmots and have more advantageous habitat conditions compared with other grassland types. The elevation of alpine meadows is 3236–5126 m, and the vegetation is mainly meadows with simple vegetation structure, substantial vegetation cover and dense vegetation growth, and a wide variety of plants, rich food, soft grass, and good palatability. Therefore, alpine meadows provide good natural habitats and foraging sites for marmots.Habitat selection of large rodents is influenced by a combination of vegetation cover availability, food availability, and population density35. Vegetation cover is an important parameter that describes vegetation communities and ecosystems and is closely related to vegetation quantity and productivity. The quality of habitat vegetation is an important factor that affects the spatial distribution of plateau rodents. Both feeding and concealment depend on vegetation, and the height and cover of edible plants and vegetation suitable for concealment determine the choice of vegetation type by marmots. Thus, vegetation cover becomes an important factor for habitat selection by marmots. Different grassland types determine different plant conditions, and selection of different vegetation conditions can increase the chances of survival and improve the reproductive success of marmots; therefore, grassland type is an important ecological factor in habitat selection by marmots. A study showed that the ecological factors affecting habitat selection of Himalayan marmots are mainly topography, anthropogenic disturbance, and vegetation8. Another study concluded that habitat selection by Himalayan marmots is closely related to elements such as topography, landform, temperature, precipitation, and vegetation24.The functions of burrows’ physical parameters is to protect the Himalayan marmots from natural enemies and bad weather36. There is clearly influence of slope on habitat selection by marmots. When the slope is large, wind is strong, and burrows are not well hidden; this makes them difficult to defend against enemies, unsafe for survival, and not conducive to hibernation during winter. In addition, Himalayan marmots prefer to burrow on sunny aspect, because the temperature is suitable and the vegetation is lush, which is suitable for marmots to breed. Therefore, the number of marmot burrows gradually decreases with increasing slope and ubac. Although flat and low-lying areas with small slopes are good for marmots to create dens, rainwater will easily flow into the dens during summer rainfall, which will kill marmots. Therefore, a suitable slope and sunny aspect are also very important for habitat selection by marmots.Application of the predictive spatial distribution map of Himalayan marmots in Qinghai provincePlague surveillance is the main measure used for plague prevention and control in China. Although we have made many improvements in plague surveillance, the traditional method of dragnet surveillance still consumes a lot of human and material resources, is inefficient. The pasture area of Qinghai province is approximately 380,000 km2, and the identified natural plague focus is approximately 180,000 km2; therefore, there is still 200,000 km2 of pasture where the distribution of Himalayan marmots and plague have not been identified. Currently, RS technology is widely used in the fields of mapping and ecological surveillance18,19,21,22,37.Applications of RS technology in areas such as malaria, dengue, schistosomiasis and plague have been previously reported27,37. Using GIS combined with remotely sensed data, Proches Hieronimo et al. found that the presence of small mammals was positively influenced by elevation, whereas the presence of fleas was clearly influenced by land management features, and thus these observations have positive implications for plague surveillance27. In this study, RS technology combined with field validations were used to determine the distribution and areas of different types of grasslands in Qinghai province, and the average density of Himalayan marmot distribution in different types of grasslands. The high-, low-, and very low-density areas of Himalayan marmot distribution were identified. The soil map, vegetation map, administrative map, and marmot density statistics were merged to form the spatial data and attribute data basis for the information system to map the distribution of Himalayan marmot and determine the area of Himalayan marmot distribution. Generally speaking, the occurrence of human plague epidemic is closely related to the local animal plague epidemic2. However, a large part of the high-density distribution of Himalayan marmots is located in uninhabited areas and the areas are generally sparsely populated, which also indicates that we should reasonably allocate plague prevention and control resources to areas where human plague is most likely to occur to prevent the occurrence of human plague epidemics.Field validation for verificationThrough field validation and information from local farmers and herdsmen, we confirmed that Himalayan marmots inhabited 68 sample sites in Tongde, Zeku, Guinan, Xunhua, Haiyan, Ulan, Qilian, Hualong, and Huzhu counties. Among them, Tongde, Zeku, Guinan, Xunhua, Haiyan, Ulan, and Qilian counties have all historically experienced marmot plague outbreaks and can be considered as reliable natural plague foci38. The data from this field validation are consistent with the previous survey data and the epidemic history of the counties in Qinghai province39.MAE can better reflect the actual number of errors in prediction values; the smaller the MAE value, the higher the prediction accuracy. The MAE derived from the field validation data was 0.1331 and the prediction accuracy was 0.8669. The accuracy of the predicted Himalayan marmot spatial distribution reached 87%, which indicated that the predicted probability map of the Himalayan marmot spatial distribution can better predict the potential marmot distribution.The predicted spatial distribution map of Himalayan marmot in Qinghai province was then compared with environmental information such as elevation, vegetation, grass type, slope, and aspect of 352 field survey sites. The obtained RS data showed that the prediction results were excellent, and the predicted spatial distribution map of Himalayan marmot in Qinghai province was drawn with high accuracy. The prediction map visually reflects the different density distribution of Himalayan marmots; this allows us to optimize the settings and reasonable spatial layout of animal plague surveillance sites and improve surveillance efficiency.Application of marmot information collection system V3.0Marmot information collection system V3.0 was developed based on the “3S” technology standardizing the collection of surveillance data, and makes the management and analysis of information more convenient and faster. This study revolutionized the traditional method of considering plague-stricken counties as the plague foci, and effectively reduces the work intensity of operators and improves the data collection efficiency. In 2016 and 2017, we applied this system to the animal plague surveillance tasks in the plague-stricken counties of Haidong, Hainan, and Haibei in Qinghai province, and standardized the collection of provincial geographic location data of animal plague surveillance (data not shown). In 2018, we also applied this system in Wulan County, which frequently experiences plague, and achieved a good application effect (data not shown).In the next step, we will expand the pilot areas (mainly national and provincial plague surveillance sites), collect surveillance data from each surveillance site, continuously optimize and update the system, improve the efficiency of data analysis and utilization, detect the plague epidemic in marmot in a timely and accurate manner, correctly determine the epidemic trend of plague in marmots, and attempt to strictly prevent the plague from spreading to humans. We plan to use a new model of drone surveillance to create a multidimensional, three-dimensional, real-time big data plague surveillance information reporting system to enhance early plague warnings and prediction in Qinghai province and even in the country, which will be of positive practical significance to serve and guarantee the Belt and Road Initiative. These approaches are expected to provide new technical means for plague investigation and research, and to provide references for setting up plague surveillance programs and prediction for the natural Himalayan marmot plague focus in Qinghai province and the QTP. More

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    Heterogeneity of interaction strengths and its consequences on ecological systems

    Now consider a generalized model in which the species interactions are heterogeneous. A natural way of introducing heterogeneity in the system is by having a species diversify into subpopulations with different interaction strengths12,13,14,15. This way of modeling heterogeneity is useful as it can describe different kinds of heterogeneity. For example, the subpopulations could represent polymorphic traits that are genetically determined or result from plastic response to heterogeneous environments. A population could also be divided into local subpopulations in different spatial patches, which can migrate between patches and may face different local predators. We can also model different behavioral modes as subpopulations that, for instance, spend more time foraging for food or hiding from predators. We study several kinds of heterogeneity after we introduce a common mathematical framework. By studying these different scenarios using variants of the model, we show that our main results are not sensitive to the details of the model.We focus on the simple case where only the prey species splits into two types, (C_1) and (C_2), as illustrated in Fig. 1b. The situation is interesting when predator A consumes (C_1) more readily than predator B and B consumes (C_2) more readily than A (i.e., (a_1 / a_0 > b_1 / b_0) and (b_2 / b_0 > a_2 / a_0), which is equivalent to the condition that the nullclines of A and B cross, see section “Resources competition and nullcline analysis”). The arrows between (C_1) and (C_2) in Fig. 1b represent the exchange of individuals between the two subpopulations, which can happen by various mechanisms considered below. Such exchange as well as intraspecific competition between (C_1) and (C_2) result from the fact that the two prey types remain a single species.The system is now described by an enlarged Lotka-Volterra system with four variables, A, B, (C_1), and (C_2): $$begin{aligned} dot{A}&= varepsilon _A ,alpha _{A1} , A , C_1 + alpha _{A2} , A , C_2 – beta _A , A end{aligned}$$
    (3a)
    $$begin{aligned} dot{B}&= varepsilon _B , alpha _{B1} , B , C_1 + alpha _{B2} , B , C_2 – beta _B , B end{aligned}$$
    (3b)
    $$begin{aligned} dot{C_1}&= C_1 , (beta _C – alpha _{CC} , C)-alpha _{A1} , C_1 A-alpha _{B1} , C_1 B – sigma _1 , C_1 + sigma _2 , C_2 end{aligned}$$
    (3c)
    $$begin{aligned} dot{C_2}&= C_2 , (beta _C – alpha _{CC} , C) -alpha _{A2} , C_2 A -alpha _{B2} , C_2 B + sigma _1 , C_1 – sigma _2 , C_2 end{aligned}$$
    (3d)
    The parameters in these equations and their meanings are listed in Table 1. Here we assume that the prey types (C_1) and (C_2) have the same birth rate and intraspecific competition strength, but different interaction strengths with A and B. Note that (C_1) and (C_2) are connected by the (sigma _i) terms, which represent the exchange of individuals between these subpopulations through mechanisms studied below; these terms indicate a major difference between our model of a prey with intraspecific heterogeneity and other models of two prey species. For the convenience of analysis, we transform the variables (C_1) and (C_2) to another pair of variables C and (lambda), where (C equiv C_1 + C_2) is the total population of C as before, and (lambda equiv C_2 / (C_1 + C_2)) represents the composition of the population (Fig. 1c). After this transformation and rescaling of variables (described in “Methods”), the new dynamical system can be written as: $$begin{aligned} dot{A}&= A , big ( C , (a_1 (1-lambda ) + a_2 lambda ) – a_0 big ) end{aligned}$$
    (4a)
    $$begin{aligned} dot{B}&= B , big ( C , (b_1 (1-lambda ) + b_2 lambda ) – b_0 big ) end{aligned}$$
    (4b)
    $$begin{aligned} dot{C}&= C , big ( 1 – C – A (a_1 (1-lambda ) + a_2 lambda ) – B (b_1 (1-lambda ) + b_2 lambda ) big ) end{aligned}$$
    (4c)
    $$begin{aligned} dot{lambda }&= lambda (1-lambda ) , big ( A (a_1 – a_2) + B (b_1 – b_2) big ) + eta _1 (1-lambda ) – eta _2 lambda end{aligned}$$
    (4d)
    Here, (a_i) and (b_i) are the (rescaled) feeding rates of the predators on the prey type (C_i); (a_0) and (b_0) are the death rates of the predators as before; (eta _1) and (eta _2) are the exchange rates of the prey types (Table 1). The latter can be functions of other variables, representing different kinds of heterogeneous interactions that we study below. Notice that Eqs. (4a–4c) are equivalent to the homogeneous Eqs. (2a–2c) but with effective interaction strengths (a_text {eff} = (1-lambda ) , a_1 + lambda , a_2) and (b_text {eff} = (1-lambda ) , b_1 + lambda , b_2) that both depend on the prey composition (lambda) (Fig. 1c).Table 1 Model parameters (before/after rescaling) and their meanings.Full size tableThe variable (lambda) can be considered an internal degree of freedom within the C population. In all of the models we study below, (lambda) dynamically stabilizes to a special value (lambda ^*) (a bifurcation point), as shown in Fig. 3a. Accordingly, a new equilibrium point (P_N) appears (on the line (mathscr {L}) in Fig. 2), at which all three species coexist. For comparison, Fig. 3b shows the equilibrium points if (lambda) is held fixed at any other values, which all result in the exclusion of one of the predators. Thus, heterogeneous interactions give rise to a new coexistence phase (see Fig. 4 below) by bringing the prey composition (lambda) to the value (lambda ^*), instead of having to fine-tune the interaction strengths. The exact conditions for this new equilibrium to be stable are detailed in “Methods”.Figure 3(a) Time series of (lambda) for systems with each kind of heterogeneity. All three systems stabilize at the same (lambda ^*) value, which is the bifurcation point in panel (b). (b) Equilibrium population of each species (X = A), B, or C, with (lambda) held fixed at different values. Solid curves represent stable equilibria and dashed curves represent unstable equilibria (see Eq. (9) in “Methods”). The vertical dashed line is where (lambda = lambda ^*), which is also the bifurcation point. Notice that the equilibrium population of C is maximized at this point (for (a_1 > a_2) and (b_2 > b_1)). Parameters used here are ((a_0, a_1, a_2, b_0, b_1, b_2, rho , eta _1, eta _2, kappa ) = (0.25, 0.5, 0.2, 0.4, 0.2, 0.6, 0.5, 0.05, 0.05, 50)).Full size imageInherent heterogeneityWe first consider a scenario where individuals of the prey species are born as one of two types with a fixed ratio, such that a fraction (rho) of the newborns are (C_2) and ((1-rho )) are (C_1). This could describe dimorphic traits, such as the winged and wingless morphs in aphids12 or the horned and hornless morphs in beetles13. We call this “inherent” heterogeneity, because individuals are born with a certain type and cannot change in later stages of life. The prey type given at birth determines the individual’s interaction strength with the predators. This kind of heterogeneity can be described by Eq. (4d) with (eta _1 = rho (1-C)) and (eta _2 = (1-rho ) (1-C)) (see “Methods”).Figure 4Phase diagrams showing regions of parameter space identified by the stable equilibrium points. Yellow region represents (P_C) (predators A, B both extinct), red represents (P_A) (A excludes B), blue represents (P_B) (B excludes A), and green represents (P_N) (A, B coexist). The middle point (black dot) is where the preferences of the two predators are identical, (a_2/a_0=b_2/b_0) and (b_1/b_0=a_1/a_0). The coexistence phase appears in all three kinds of heterogeneity modeled here. (a–d) Inherent heterogeneity: Individuals of the prey population are born in two types with a fixed composition (rho). In the extreme cases of (rho = 0) and 1, the prey is homogeneous and there is no coexistence of the predators. (e–h) Reversible heterogeneity: Individual prey can switch types with fixed switching rates (eta _1) and (eta _2). As the switching rates increase, the coexistence region shrinks because the prey population becomes effectively homogeneous (the occasional green spots are numerical artifacts because the time to reach the equilibrium becomes long in this limit). (i–l) Adaptive heterogeneity: The switching rates (eta _i) dynamically adapt to the predator densities, so as to maximize the growth rate of the prey. As the sharpness (kappa) of the sigmoidal decision function is increased, the prey adapts more optimally and the region of coexistence expands. Parameters used here are ((a_0, a_1, b_0, b_2) = (0.3, 0.5, 0.4, 0.6)).Full size imageThe stable equilibrium of the system can be represented by phase diagrams that show the identities of the species at equilibrium. We plot these phase diagrams by varying the parameters (a_2) and (b_1) while keeping (a_1) and (b_2) constant. As shown in Fig. 4a–d, the equilibrium state depends on the parameter (rho). In the limit (rho = 0) or 1, we recover the homogeneous case because only one type of C is produced. The corresponding phase diagrams (Fig. 4a, d) contain only two phases where either of the predators is excluded, illustrating the competitive exclusion principle. For intermediate values of (rho), however, there is a new phase of coexistence that separates the two exclusion phases (Fig. 4b, c). There are two such regions of coexistence, which touch at a middle point and open toward the bottom left and upper right, respectively. The middle point is at ((a_2/a_0 = b_2/b_0, b_1/b_0 = a_1/a_0)), where the feeding preferences of the two predators are identical (hence their niches fully overlap). Towards the origin and the far upper right, the predators consume one type of C each (hence their niches separate). The coexistence region in the bottom left is where the feeding rates of the predators are the lowest overall. There can be a region (yellow) where both predators go extinct, if their primary prey type alone is not enough to sustain each predator. Increasing the productivity of the system by increasing the birth rate ((beta _C)) of the prey eliminates this extinction region, whereas lowering productivity causes the extinction region to take over the lower coexistence region. Because the existence and identity of the phases is determined by the configuration of the equilibrium points (Fig. 2, see also section “Mathematical methods”), the qualitative shape of the phase diagram is not sensitive to changes of parameter values.The new equilibrium is not only where the predators A and B can coexist, but also where the prey species C grows to a larger density than what is possible for a homogeneous population. This is illustrated in Fig. 3b, which shows the equilibrium population of C if we hold (lambda) fixed at different values. The point (lambda = lambda ^*) is where the system with a dynamic (lambda) is stable, and also where the population of C is maximized (when A and B prefer different prey types). That means the population automatically stabilizes at the optimal composition of prey types. Moreover, the value of (C^*) at this coexistence point can even be larger than the equilibrium population of C when there is only one predator A or B. This is discussed further in section “Multiple-predator effects and emergent promotion of prey”. These results suggest that heterogeneity in interaction strengths can potentially be a strategy for the prey population to leverage the effects of multiple predators against each other to improve survival.Reversible heterogeneityWe next consider a scenario where individual prey can switch their types. This kind of heterogeneity can model reversible changes of phenotypes, i.e., trait changes that affect the prey’s interaction with predators but are not permanent. For example, changes in coat color or camouflage14,16,17, physiological changes such as defense15, and biomass allocation among tissues18,19. One could also think of the prey types as subpopulations within different spatial patches, if each predator hunts at a preferred patch and the prey migrate between the patches20,21. With some generalization, one could even consider heterogeneity in resources, such as nutrients located in different places, that can be reached by primary consumers, such as swimming phytoplankton22. We can model this “reversible” kind of heterogeneity by introducing switching rates from one prey type to the other. In Eq. (4d), (eta _1) and (eta _2) now represent the switching rates per capita from (C_1) to (C_2) and from (C_2) to (C_1), respectively. Here we study the simplest case where both rates are fixed.In the absence of the predators, the natural composition of the prey species given by the switching rates would be (rho equiv eta _1 / (eta _1 + eta _2)), and the rate at which (lambda) relaxes to this natural composition is (gamma equiv eta _1 + eta _2). Compared to the previous scenario where we had only one parameter (rho), here we have an additional parameter (gamma) that modifies the behavior of the system. Fig. 4e–h shows phase diagrams for the system as (rho) is fixed and (gamma) varies. We again find the new equilibrium (P_N) where all three species coexist. When (gamma) is small, the system has a large region of coexistence. As (gamma) is increased, this region is squeezed into a border between the two regions of exclusion, where the slope of the border is given by (eta _1/eta _2) as determined by the parameter (rho). However, this is different from the exclusion we see in the case of inherent heterogeneity, which happens only for (rho rightarrow 0) or 1, where the borders are horizontal or vertical (Fig. 4a,d). Here the predators exclude each other despite having a mixture of prey types in the population.This special limit can be understood as follows. For a large (gamma), (lambda) is effectively set to a constant value equal to (rho), because it has a very fast relaxation rate. In other words, the prey types exchange so often that the population always maintains a constant composition. In this limit, the system behaves as if it were a homogeneous system with effective interaction strengths (a_text {eff} = (1-rho ) , a_1 + rho , a_2) and (b_text {eff} = (1-rho ) , b_1 + rho , b_2). As in a homogeneous system, there is competitive exclusion between the predators instead of coexistence. This demonstrates that having a constant level of heterogeneity is not sufficient to cause coexistence. The overall composition of the population must be able to change dynamically as a result of population growth and consumption by predators.An interesting behavior is seen when we examine a point inside the shrinking coexistence region as (gamma) is increased. Typical trajectories of the system for such parameter values are shown in Fig. 5. As (gamma) increases, the system relaxes to the line (mathscr {L}) quickly, then slowly crawls along it towards the final equilibrium point (P_N). This is because increasing (gamma) increases the speed that (lambda) relaxes to (lambda ^*), and when (lambda rightarrow lambda ^*), (mathscr {L}) becomes marginally stable. Therefore, the attraction to (mathscr {L}) in the perpendicular direction is strong, but the attraction towards the equilibrium point along (mathscr {L}) is weak. This leads to a long transient behavior that makes the system appear to reach no equilibrium in a limited time23,24. It is especially true when there is noise in the dynamics, which causes the system to diffuse along (mathscr {L}) with only a weak drift towards the final equilibrium (Fig. 5). Thus, the introduction of a fast timescale (quick relaxation of (lambda) due to a large (gamma)) actually results in a long transient.Figure 5Trajectories of the system projected in the A-B plane, with parameters inside the coexistence region (by holding the position of (P_N) fixed). As (gamma) increases, the system tends to approach the line (mathscr {L}) quickly and then crawl along it. The grey trajectory is with independent Gaussian white noise ((sim mathscr {N}(0,0.5))) added to each variable’s dynamics. Noise causes the system to diffuse along (mathscr {L}) for a long transient period before coming to the equilibrium point (P_N). Parameters used here are ((a_0, a_1, a_2, b_0, b_1, b_2) = (0.2, 0.8, 0.5, 0.2, 0.6, 0.9)), chosen to place (P_N) away from the middle of (mathscr {L}) to show the trajectory drifting toward the equilibrium.Full size imageAdaptive heterogeneityA third kind of heterogeneity we consider is the change of interactions in time. By this we mean an individual can actively change its interaction strength with others in response to certain conditions. This kind of response is often invoked in models of adaptive foraging behavior, where individuals choose appropriate actions to maximize some form of fitness25,26. For example, we may consider two behaviors, resting and foraging, as our prey types. Different predators may prefer to strike when the prey is doing different things. In response, the prey may choose to do one thing or the other depending on the current abundances of different predators. Such behavioral modulation is seen, for example, in systems of predatory spiders and grasshoppers27. Phenotypic plasticity is also seen in plant tissues in response to consumers28,29,30.This kind of “adaptive” heterogeneity can be modeled by having switching rates (eta _1) and (eta _2) that are time-dependent. Let us assume that the prey species tries to maximize its population growth rate by switching to the more favorable type. From Eq. (4c), we see that the growth rate of C depends linearly on the composition (lambda) with a coefficient (u(A,B) equiv (a_1 – a_2) A + (b_1 – b_2) B). Therefore, when this coefficient is positive, it is favorable for C to increase (lambda) by switching to type (C_2). This can be achieved by having a positive switching rate (eta _2) whenever (u(A,B) > 0). Similarly, whenever (u(A,B) < 0), it is favorable for C to switch to type (C_1) by having a positive (eta _1). In this way, the heterogeneity of the prey population constantly adapts to the predator densities. We model such adaptive switching by making (eta _1) and (eta _2) functions of the coefficient u(A, B), e.g., (eta _1(u) = 1/(1+mathrm {e}^{kappa u})) and (eta _2(u) = 1/(1+mathrm {e}^{-kappa u})). The sigmoidal form of the functions means that the switching rate in the favorable direction for C is turned on quickly, while the other direction is turned off. The parameter (kappa) controls the sharpness of this transition.Phase diagrams for the system with different values of (kappa) are shown in Fig. 4i–l. A larger (kappa) means the prey adapts its composition faster and more optimally, which causes the coexistence region to expand. In the extreme limit, the system changes its dynamics instantaneously whenever it crosses the boundary where (u(A,B) = 0), like in a hybrid system31. Such a system can still reach a stable equilibrium that lies on the boundary, if the flow on each side of the boundary points towards the other side32. This is what happens in our system and, interestingly, the equilibrium is the same three-species coexistence point (P_N) as in the previous scenarios. The region of coexistence turns out to be largest in this limit (Fig. 4l).Our results suggest that the coexistence of the predators can be viewed as a by-product of the prey’s strategy to maximize its own benefit. The time-dependent case studied here represents a strategy that involves the prey evaluating the risk posed by different predators. This is in contrast to the scenarios studied above, where the prey population passively creates phenotypic heterogeneity regardless of the presence of the predators. These two types of behavior are analogous to the two strategies studied for adaptation in varying environments, i.e., sensing and bet-hedging33,34. The former requires accessing information about the current environment to make optimal decisions, whereas the latter relies on maintaining a diverse population to reduce detrimental effects caused by environmental changes. Here the varying abundances of the predators play a similar role as the varying environment. From this point of view, the heterogeneous interactions studied here can be a strategy of the prey species that is evolutionarily favorable. More