Ibisch, P. L. et al. A global map of roadless areas and their conservation status. Science 354, 1423–1427 (2016).
Google Scholar
Torres, A., Jaeger, J. A. G. & Alonso, J. C. Assessing large-scale wildlife responses to human infrastructure development. PNAS 113, 8472–8477 (2016).
Google Scholar
Bennett, V. J. Effects of road density and pattern on the conservation of species and biodiversity. Curr. Landsc. Ecol. Rep. 2, 1–11 (2017).
Google Scholar
Coffin, A. W. From roadkill to road ecology: a review of the ecological effects of roads. J. Transp. Geogr. 15, 396–406 (2007).
Google Scholar
Fahrig, L. & Rytwinski, T. Effects of roads on animal abundance: an empirical review and synthesis. Ecol. Soc. 14, 21 (2009).
Google Scholar
Forman, R. T. & Alexander, L. E. Roads and their major ecological effects. Annu. Rev. Ecol. Syst. 29, 207–231 (1998).
Google Scholar
Trombulak, S. C. & Frissell, C. A. Review of ecological effects of roads on terrestrial and aquatic communities. Conserv. Biol. 14, 18–30 (2000).
Google Scholar
Ascensão, F. et al. Disentangle the causes of the road barrier effect in small mammals through genetic patterns. PLoS ONE 11, e0151500 (2016).
Google Scholar
Dyer, S. J., O’Neill, J. P., Wasel, S. M. & Boutin, S. Quantifying barrier effects of roads and seismic lines on movements of female woodland caribou in northeastern Alberta. Can. J. Zool. 80, 839–845 (2002).
Google Scholar
Glista, D. J., DeVault, T. L. & DeWoody, J. A. A review of mitigation measures for reducing wildlife mortality on roadways. Landsc. Urban Plan. 91, 1–7 (2009).
Google Scholar
Taylor, B. D. & Goldingay, R. L. Roads and wildlife: impacts, mitigation and implications for wildlife management in Australia. Wildl. Res. 37, 320–331 (2010).
Google Scholar
Tigas, L. A., Van Vuren, D. H. & Sauvajot, R. M. Behavioral responses of bobcats and coyotes to habitat fragmentation and corridors in an urban environment. Biol. Conserv. 108, 299–306 (2002).
Google Scholar
Herrmann, H.-W., Pozarowski, K. M., Ochoa, A. & Schuett, G. W. An interstate highway affects gene flow in a top reptilian predator (Crotalus atrox) of the Sonoran Desert. Conserv. Genet. 18, 911–924 (2017).
Google Scholar
Holderegger, R. & Di Giulio, M. The genetic effects of roads: a review of empirical evidence. Basic Appl. Ecol. 11, 522–531 (2010).
Google Scholar
Keller, L. F. & Waller, D. M. Inbreeding effects in wild populations. Trends Ecol. Evol. 17, 230–241 (2002).
Google Scholar
Benítez-López, A., Alkemade, R. & Verweij, P. A. The impacts of roads and other infrastructure on mammal and bird populations: a meta-analysis. Biol. Conserv. 143, 1307–1316 (2010).
Google Scholar
Kerley, L. L. et al. Effects of roads and human disturbance on Amur tigers. Conserv. Biol. 16, 97–108 (2002).
Google Scholar
Roedenbeck, I. A. & Voser, P. Effects of roads on spatial distribution, abundance and mortality of brown hare (Lepus europaeus) in Switzerland. Eur. J. Wildl. Res. 54, 425–437 (2008).
Google Scholar
Clark, R. W., Brown, W. S., Stechert, R. & Zamudio, K. R. Roads, interrupted dispersal, and genetic diversity in timber Rattlesnakes. Conserv. Biol. 24, 1059–1069 (2010).
Google Scholar
Niko, B. & Waits, L. P. Molecular road ecology: exploring the potential of genetics for investigating transportation impacts on wildlife. Mol. Ecol. 18, 4151–4164 (2009).
Google Scholar
Frankham, R., Briscoe, D. A. & Ballou, J. D. Introduction to Conservation Genetics (Cambridge University Press, 2002).
Google Scholar
Amos, W. & Balmford, A. When does conservation genetics matter?. Heredity 87, 257–265 (2001).
Google Scholar
Frankham, R. Genetics and extinction. Biol. Cons. 126, 131–140 (2005).
Google Scholar
Teixeira, J. C. & Huber, C. D. The inflated significance of neutral genetic diversity in conservation genetics. Proc. Natl. Acad. Sci. U. S. A. 118, e2015096118 (2021).
Google Scholar
Keller, I. & Largiadèr, C. R. Recent Habitat fragmentation caused by major roads leads to reduction of gene flow and loss of genetic variability in ground beetles. Proc. Biol. Sci. 270, 417–423 (2003).
Google Scholar
Noël, S., Ouellet, M., Galois, P. & Lapointe, F.-J. Impact of urban fragmentation on the genetic structure of the eastern red-backed salamander. Conserv. Genet. 8, 599–606 (2007).
Google Scholar
Marsh, D. M. et al. Effects of roads on patterns of genetic differentiation in red-backed salamanders, Plethodon cinereus. Conserv. Genet. 9, 603–613 (2008).
Google Scholar
Brehme, C. S., Tracey, J. A., Mcclenaghan, L. R. & Fisher, R. N. Permeability of roads to movement of scrubland lizards and small mammals. Conserv. Biol. 27, 710–720 (2013).
Google Scholar
Ford, A. T. & Clevenger, A. P. Factors affecting the permeability of road mitigation measures to the movement of small mammals. Can. J. Zool. 97, 379–384 (2018).
Google Scholar
Claireau, F. et al. Major roads have important negative effects on insectivorous bat activity. Biol. Conserv. 235, 53–62 (2019).
Google Scholar
Jacobson, S. L., Bliss-Ketchum, L. L., Rivera, C. E. & Smith, W. P. A behavior-based framework for assessing barrier effects to wildlife from vehicle traffic volume. Ecosphere 7, e01345 (2016).
Google Scholar
Assis, J. C., Giacomini, H. C. & Ribeiro, M. C. Road permeability index: evaluating the heterogeneous permeability of roads for wildlife crossing. Ecol. Ind. 99, 365–374 (2019).
Google Scholar
Lesbarrères, D., Primmer, C. R., Lodé, T. & Merilä, J. The effects of 20 years of highway presence on the genetic structure of Rana dalmatina populations. Écoscience 13, 531–538 (2006).
Google Scholar
Landguth, E. L. et al. Quantifying the lag time to detect barriers in landscape genetics: quantifying the lag time to detect barriers in landscape genetics. Mol. Ecol. 19, 4179–4191 (2010).
Google Scholar
Epps, C. W. & Keyghobadi, N. Landscape genetics in a changing world: disentangling historical and contemporary influences and inferring change. Mol. Ecol. 24, 6021–6040 (2015).
Google Scholar
Blair, C. et al. A simulation-based evaluation of methods for inferring linear barriers to gene flow. Mol. Ecol. Resour. 12, 822–833 (2012).
Google Scholar
Mona, S., Ray, N., Arenas, M. & Excoffier, L. Genetic consequences of habitat fragmentation during a range expansion. Heredity 112, 291–299 (2014).
Google Scholar
Weyer, J., Jørgensen, D., Schmitt, T., Maxwell, T. J. & Anderson, C. D. Lack of detectable genetic differentiation between den populations of the Prairie Rattlesnake (Crotalus viridis) in a fragmented landscape. Can. J. Zool. 92, 837–846 (2014).
Google Scholar
Frankham, R. Relationship of genetic variation to population size in wildlife. Conserv. Biol. 10, 1500–1508 (1996).
Google Scholar
Ehrich, D. & Jorde, P. E. High genetic variability despite high-amplitude population cycles in lemmings. J. Mammal. 86, 380–385 (2005).
Google Scholar
Gauffre, B. et al. Short-term variations in gene flow related to cyclic density fluctuations in the common vole. Mol. Ecol. 23, 3214–3225 (2014).
Google Scholar
Schweizer, M., Excoffier, L. & Heckel, G. Fine-scale genetic structure and dispersal in the common vole (Microtus arvalis). Mol. Ecol. 16, 2463–2473 (2007).
Google Scholar
Keane, B., Ross, S., Crist, T. O. & Solomon, N. G. Fine-scale spatial patterns of genetic relatedness among resident adult prairie voles. J. Mammal. 96, 1194–1202 (2015).
Google Scholar
Boyce, C. C. K. & Boyce, J. L. Population biology of Microtus arvalis. II. Natal and breeding dispersal of females. J. Anim. Ecol. 57, 723–736 (1988).
Google Scholar
Luque-Larena, J. J. et al. Recent large-scale range expansion and outbreaks of the common vole (Microtus arvalis) in NW Spain. Basic Appl. Ecol. 14, 432–441 (2013).
Google Scholar
Salamolard, M., Butet, A., Leroux, A. & Bretagnolle, V. Responses of an avian predator to variations in prey density at a temperate latitude. Ecology 81, 2428–2441 (2000).
Google Scholar
Krebs, C. J. & Myers, J. H. Population cycles in small mammals. In Advances in Ecological Research Vol. 8 (ed. MacFadyen, A.) 267–399 (Academic Press, 1974).
Gerlach, G. & Musolf, K. Fragmentation of landscape as a cause for genetic subdivision in bank voles. Conserv. Biol. 14, 1066–1074 (2000).
Google Scholar
Rico, A., Kindlmann, P. & Sedláček, F. Can the barrier effect of highways cause genetic subdivision in small mammals?. Acta Theriol. 54, 297–310 (2009).
Google Scholar
Nei, M., Maruyama, T. & Chakraborty, R. The bottleneck effect and genetic variability in populations. Evolution 29, 1–10 (1975).
Google Scholar
Motro, U. & Thomson, G. On heterozygosity and the effective size of populations subject to size changes. Evolution 36, 1059–1066 (1982).
Google Scholar
Kirkpatrick, M. & Jarne, P. The effects of a bottleneck on inbreeding depression and the genetic load. Am. Nat. 155, 154–167 (2000).
Google Scholar
Xenikoudakis, G. et al. Consequences of a demographic bottleneck on genetic structure and variation in the Scandinavian brown bear. Mol. Ecol. 24, 3441–3454 (2015).
Google Scholar
Parra, G. J. et al. Low genetic diversity, limited gene flow and widespread genetic bottleneck effects in a threatened dolphin species, the Australian humpback dolphin. Biol. Conserv. 220, 192–200 (2018).
Google Scholar
Berthier, K., Charbonnel, N., Galan, M., Chaval, Y. & Cosson, J.-F. Migration and recovery of the genetic diversity during the increasing density phase in cyclic vole populations. Mol. Ecol. 15, 2665–2676 (2006).
Google Scholar
Norén, K. & Angerbjörn, A. Genetic perspectives on northern population cycles: bridging the gap between theory and empirical studies: GENETIC structure in cyclic populations. Biol. Rev. 89, 493–510 (2014).
Google Scholar
Jangjoo, M., Matter, S. F., Roland, J. & Keyghobadi, N. Demographic fluctuations lead to rapid and cyclic shifts in genetic structure among populations of an alpine butterfly, Parnassius smintheus. J. Evol. Biol. 33, 668–681 (2020).
Google Scholar
ESRI. ArcGIS desktop: release 10.3 Environmental Systems Research Institute, Redlands, CA (2015).
Saunders, S. C., Mislivets, M. R., Chen, J. & Cleland, D. T. Effects of roads on landscape structure within nested ecological units of the Northern Great Lakes Region, USA. Biol. Conserv. 103, 209–225 (2002).
Google Scholar
Fahrig, L. Effects of habitat fragmentation on biodiversity. Annu. Rev. Ecol. Evol. Syst. 34, 487–515 (2003).
Google Scholar
Schlaepfer, D. R., Braschler, B., Rusterholz, H.-P. & Baur, B. Genetic effects of anthropogenic habitat fragmentation on remnant animal and plant populations: a meta-analysis. Ecosphere 9, e02488 (2018).
Google Scholar
Charlesworth, B. Effective population size and patterns of molecular evolution and variation. Nat. Rev. Genet. 10, 195–205 (2009).
Google Scholar
Compton, B. W., McGarigal, K., Cushman, S. A. & Gamble, L. R. A resistant-Kernel model of connectivity for amphibians that breed in vernal pools. Conserv. Biol. 21, 788–799 (2007).
Google Scholar
Balkenhol, N., Cushman, S., Storfer, A. & Waits, L. Landscape Genetics: Concepts, Methods, Applications (Wiley, 2015).
Google Scholar
Aguilar, R., Quesada, M., Ashworth, L., Herrerias-Diego, Y. & Lobo, J. Genetic consequences of habitat fragmentation in plant populations: susceptible signals in plant traits and methodological approaches. Mol. Ecol. 17, 5177–5188 (2008).
Google Scholar
Lloyd, M. W., Campbell, L. & Neel, M. C. The Power to Detect Recent Fragmentation Events Using Genetic Differentiation Methods. PLoS ONE 8, e63981 (2013).
Google Scholar
Smouse, P. E., Long, J. C. & Sokal, R. R. Multiple regression and correlation extensions of the mantel test of matrix correspondence. Syst. Zool. 35, 627–632 (1986).
Google Scholar
Nowak, R. M. Walker’s Mammals of the World (Johns Hopkins University Press, 1999).
García, J. T. et al. A complex scenario of glacial survival in Mediterranean and continental refugia of a temperate continental vole species (Microtus arvalis) in Europe. J. Zool. Syst. Evol. Res. 58, 459–474 (2020).
Google Scholar
Martínková, N. et al. Divergent evolutionary processes associated with colonization of offshore islands. Mol. Ecol. 22, 5205–5220 (2013).
Google Scholar
Keyghobadi, N. K. The genetic implications of habitat fragmentation for animals. Can. J. Zool. 85(10), 1049–1064 (2007).
Google Scholar
Vandergast, A. G., Bohonak, A. J., Weissman, D. B. & Fisher, R. N. Understanding the genetic effects of recent habitat fragmentation in the context of evolutionary history: phylogeography and landscape genetics of a southern California endemic Jerusalem cricket (Orthoptera: Stenopelmatidae: Stenopelmatus). Mol. Ecol. 16, 977–992 (2007).
Google Scholar
Zellmer, A. J. & Knowles, L. L. Disentangling the effects of historic vs. contemporary landscape structure on population genetic divergence. Mol. Ecol. 18, 3593–3602 (2009).
Google Scholar
Delaney, K. S., Riley, S. P. D. & Fisher, R. N. A rapid, strong, and convergent genetic response to urban habitat fragmentation in four divergent and widespread vertebrates. PLoS ONE 5, e12767 (2010).
Google Scholar
Tkadlec, E. & Stenseth, N. C. A new geographical gradient in vole population dynamics. Proc. R. Soc. Lond. Ser. B Biol. Sci. 268, 1547–1552 (2001).
Google Scholar
Lambin, X., Bretagnolle, V. & Yoccoz, N. G. Vole population cycles in northern and southern Europe: Is there a need for different explanations for single pattern?. J. Anim. Ecol. 75, 340–349 (2006).
Google Scholar
Ehrich, D. & Stenseth, N. C. Genetic structure of Siberian lemmings (Lemmus sibiricus) in a continuous habitat: large patches rather than isolation by distance. Heredity 86, 716–730 (2001).
Google Scholar
Gauffre, B., Estoup, A., Bretagnolle, V. & Cosson, J. F. Spatial genetic structure of a small rodent in a heterogeneous landscape. Mol. Ecol. 17, 4619–4629 (2008).
Google Scholar
Galliard, J.-F.L., Rémy, A., Ims, R. A. & Lambin, X. Patterns and processes of dispersal behaviour in arvicoline rodents. Mol. Ecol. 21, 505–523 (2012).
Google Scholar
Gauffre, B., Petit, E., Brodier, S., Bretagnolle, V. & Cosson, J. F. Sex-biased dispersal patterns depend on the spatial scale in a social rodent. Proc. R. Soc. B Biol. Sci. 276, 3487 (2009).
Google Scholar
Lidicker, W.Z. Jr. The role of dispersal in the demography of small mammals, in Small Mammals: their production and population dynamics. Eds F.B. Golley, K. Petrusewicz and L. Ryszkowski, 103–28 (Cambridge University Press, London, 1975).
Gaines, M. S. & McClenaghan, L. R. Dispersal in small mammals. Annu. Rev. Ecol. Syst. 11, 163–196 (1980).
Google Scholar
Swingland, I. R. & Greenwood, P. J. The Ecology of Animal Movement (Clarendon Press, 1983).
Burton, C., Krebs, C. J. & Taylor, E. B. Population genetic structure of the cyclic snowshoe hare (Lepus americanus) in southwestern Yukon, Canada. Mol. Ecol. 11, 1689–1701 (2002).
Google Scholar
Plante, Y., Boag, P. T. & White, B. N. Microgeographic variation in mitochondrial DNA of meadow voles (Microtus pennsylvanicus) in relation to population density. Evolution 43, 1522–1537 (1989).
Google Scholar
Encuesta sobre superficies y rendimientos del cultivo 2012. Catálogo de publicaciones de la Administración General del Estado (MAGRAMA, 2013).
Oñate, J. J. et al. Programa piloto de acciones de conservación de la biodiversidad en sistemas ambientales con usos agrarios en el marco del desarrollo rural. Convenio de colaboración entre la Dirección General para la Biodiversidad (Ministerio de Medio Ambiente) y el Departamento Interuniversitario de Ecología (Universidad Autónoma de Madrid, 2003).
Ji, S. et al. Impact of different road types on small mammals in Mt. Kalamaili Nature Reserve. Transp. Res. Part D Transp. Environ. 50, 223–233 (2017).
Google Scholar
Vignieri, S. N. Streams over mountains: influence of riparian connectivity on gene flow in the Pacific jumping mouse (Zapus trinotatus): connectivity patterns in pacific jumping mice. Mol. Ecol. 14, 1925–1937 (2005).
Google Scholar
Russo, I.-R.M., Sole, C. L., Barbato, M., von Bramann, U. & Bruford, M. W. Landscape determinants of fine-scale genetic structure of a small rodent in a heterogeneous landscape (Hluhluwe-iMfolozi Park, South Africa). Sci. Rep. 6, 29168 (2016).
Google Scholar
Mougeot, F., Lambin, X., Rodríguez-Pastor, R., Romairone, J. & Luque-Larena, J.-J. Numerical response of a mammalian specialist predator to multiple prey dynamics in Mediterranean farmlands. Ecology 100, e02776 (2019).
Google Scholar
Schwartz, M. K. & McKelvey, K. S. Why sampling scheme matters: the effect of sampling scheme on landscape genetic results. Conserv. Genet. 10, 441 (2009).
Google Scholar
Strauss, W. M. Preparation of genomic DNA from Mammalian tissue. Curr. Protoc. Mol. Biol. 42, 1–3 (1998).
Google Scholar
Ishibashi, Y. et al. Polymorphic microsatellite DNA markers in the field vole Microtus montebelli. Mol. Ecol. 8, 163–164 (1999).
Google Scholar
Gauffre, B., Galan, M., Bretagnolle, V. & Cosson, J. Polymorphic microsatellite loci and PCR multiplexing in the common vole, Microtus arvalis. Mol. Ecol. Notes 7, 830–832 (2007).
Google Scholar
Johnson, P. C. D. & Haydon, D. T. Software for quantifying and simulating microsatellite genotyping error. Bioinform. Biol. Insights 1, 71–75 (2009).
Google Scholar
Paradis, E. pegas: an R package for population genetics with an integrated–modular approach. Bioinformatics 26, 419–420 (2010).
Google Scholar
Brookfield, J. F. A simple new method for estimating null allele frequency from heterozygote deficiency. Mol. Ecol. 5, 453–455 (1996).
Google Scholar
Adamack, A. T. & Gruber, B. PopGenReport: simplifying basic population genetic analyses in R. Methods Ecol. Evol. 5, 384–387 (2014).
Google Scholar
Jones, O. R. & Wang, J. COLONY: a program for parentage and sibship inference from multilocus genotype data. Mol. Ecol. Resour. 10, 551–555 (2010).
Google Scholar
Keenan, K., McGinnity, P., Cross, T. F., Crozier, W. W. & Prodöhl, P. A. diveRsity: An R package for the estimation and exploration of population genetics parameters and their associated errors. Methods Ecol. Evol. 4, 782–788 (2013).
Google Scholar
Coulon, A. genhet: an easy-to-use R function to estimate individual heterozygosity. Mol. Ecol. Resour. 10, 167–169 (2010).
Google Scholar
Coltman, D. W., Pilkington, J. G. & Pemberton, J. M. Fine-scale genetic structure in a free-living ungulate population. Mol. Ecol. 12, 733–742 (2003).
Google Scholar
Amos, W. et al. The influence of parental relatedness on reproductive success. Proc. R. Soc. Lond. Ser. B Biol. Sci. 268, 2021–2027 (2001).
Google Scholar
Aparicio, J. M., Ortego, J. & Cordero, P. J. What should we weigh to estimate heterozygosity, alleles or loci? Estimating heterozygosity from neutral markers. Mol. Ecol. 15, 4659–4665 (2006).
Google Scholar
Kamvar, Z. N., Tabima, J. F. & Grünwald, N. J. Poppr: an R package for genetic analysis of populations with clonal, partially clonal, and/or sexual reproduction. PeerJ 2, e281 (2014).
Google Scholar
Peakall, R. & Smouse, P. E. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update. Bioinformatics 28, 2537–2539 (2012).
Google Scholar
Hedrick, P. W. A standardized genetic differentiation measure. Evolution 59, 1633–1638 (2005).
Google Scholar
Jost, L. GST and its relatives do not measure differentiation. Mol. Ecol. 17, 4015–4026 (2008).
Google Scholar
Meirmans, P. G. & Hedrick, P. W. Assessing population structure: FST and related measures. Mol. Ecol. Resour. 11, 5–18 (2011).
Google Scholar
Nei, M. Analysis of gene diversity in subdivided populations. PNAS 70, 3321–3323 (1973).
Google Scholar
Nei, M. & Chesser, R. K. Estimation of fixation indices and gene diversities. Ann. Hum. Genet. 47, 253–259 (1983).
Google Scholar
Bowcock, A. M. et al. High resolution of human evolutionary trees with polymorphic microsatellites. Nature 368, 455–457 (1994).
Google Scholar
Takezaki, N. & Nei, M. Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA. Genetics 144, 389–399 (1996).
Google Scholar
Smouse, P. E. & Peakall, R. Spatial autocorrelation analysis of individual multiallele and multilocus genetic structure. Heredity 82, 561–573 (1999).
Google Scholar
Queller, D. C. & Goodnight, K. F. Estimating relatedness using genetic markers. Evolution 43, 258–275 (1989).
Google Scholar
Ritland, K. Estimators for pairwise relatedness and individual inbreeding coefficients. Genet. Res. 67, 175–185 (1996).
Google Scholar
Lynch, M. & Ritland, K. Estimation of pairwise relatedness with molecular markers. Genetics 152, 1753–1766 (1999).
Google Scholar
Jombart, T. adegenet: a R package for the multivariate analysis of genetic markers. Bioinformatics 24, 1403–1405 (2008).
Google Scholar
Legendre, P. Spatial autocorrelation: Trouble or new paradigm?. Ecology 74, 1659–1673 (1993).
Google Scholar
Zuur, A., Ieno, E. N. & Smith, G. M. Analyzing Ecological Data (Springer, Berlin, 2007).
Google Scholar
Hedges, L. V. & Olkin, I. Statistical Methods for Meta-Analysis (Academic Press, 2014).
Google Scholar
Kirby, K. N. & Gerlanc, D. BootES: an R package for bootstrap confidence intervals on effect sizes. Behav. Res. Methods 45, 905–927 (2013).
Google Scholar
Cohen, J. A power primer. Psychol. Bull. 112, 155–159 (1992).
Google Scholar
Cohen, J. Statistical Power Analysis for the Behavioral Sciences (Academic Press, 2013).
Google Scholar
González-Esteban, J. & Villate I. Microtus arvalis Pallas, 1778. In: Atlas y Libro Rojo de los Mamíferos Terrestres de España, Palomo, L.J., Gisbert, J. & Blanco J.C. (Eds.), Dirección General para la Biodiversidad-SECEM-SECEMU, 426-428 (2007).
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