Centers for Disease Control and Prevention https://www.cdc.gov/dengue/areaswithrisk/index.html (2021).
Bhatt, S. et al. The global distribution and burden of dengue. Nature 496, 504–507 (2013).
Google Scholar
Brady, O. J. et al. Refining the global spatial limits of dengue virus transmission by evidence-based consensus. PLoS Negl. Trop. Dis. 6, e1760 (2012).
Google Scholar
Centers for Disease Control and Prevention https://www.cdc.gov/parasites/malaria/index.html (2021)
Gatton, M. L. et al. The importance of mosquito behavioural adaptations to malaria control in Africa. Evolution 67, 1218–1230 (2013).
Google Scholar
Sokhna, C., Ndiath, M. O. & Rogier, C. The changes in mosquito vector behaviour and the emerging resistance to insecticides will challenge the decline of malaria. Clin. Microbiol. Infect. 19, 902–907 (2013).
Google Scholar
Hemingway, J., Hawkes, N. J., McCarroll, L. & Ranson, H. The molecular basis of insecticide resistance in mosquitoes. Insect Biochem. Mol. Biol. 34, 653–665 (2004).
Google Scholar
Alphey, L. et al. Sterile-insect methods for control of mosquito-borne diseases: an analysis. Vector Borne Zoonotic Dis. 10, 295–311 (2010).
Google Scholar
Oliva, C. F., Damiens, D. & Benedict, M. Q. Male reproductive biology of Aedes mosquitoes. Acta Tropica 132, S12–S19 (2014).
Google Scholar
Benelli, G. Research in mosquito control: current challenges for a brighter future. Parasitol. Res. 114, 2801–2805 (2015).
Google Scholar
Lees, R. S., Gilles, J. R. L., Hendrichs, J., Vreysen, M. J. B. & Bourtzis, K. Back to the future: the sterile insect technique against mosquito disease vectors. Curr. Opin. Insect Sci. 10, 156–162 (2015).
Google Scholar
Wilke, A. B. & Marrelli, M. T. Genetic control of mosquitoes: population suppression strategies. Rev. Inst. Med. Trop. Sao Paulo 54, 287–292 (2012).
Google Scholar
Alphey, L., Nimmo, D., O’Connell, S. & Alphey, N. Insect population suppression using engineered insects. Adv. Exp. Med. Biol. 627, 93–103 (2008).
Google Scholar
Carvalho, D. O. et al. Suppression of a field population of Aedes aegypti in Brazil by sustained release of transgenic male mosquitoes. PLoS Negl. Trop. Dis. 9, e0003864 (2015).
Google Scholar
Wilke, A. B. B. & Marrelli, M. T. Paratransgenesis: a promising new strategy for mosquito vector control. Parasit. Vectors 8, 342 (2015).
Google Scholar
Hegde, S. & Hughes, G. L. Population modification of Anopheles mosquitoes for malaria control: pathways to implementation. Pathog. Glob. Health 111, 401–402 (2017).
Google Scholar
Carballar-Lejarazu, R. & James, A. A. Population modification of Anopheline species to control malaria transmission. Pathog. Glob. Health 111, 424–35. (2017).
Google Scholar
Li, Y. & Liu, X. A sex-structured model with birth pulse and release strategy for the spread of Wolbachia in mosquito population. J. Theor. Biol. 448, 53–65 (2018).
Google Scholar
Farkas, J. Z., Gourley, S. A., Liu, R. & Yakubu, A. A. Modelling Wolbachia infection in a sex-structured mosquito population carrying West Nile virus. J. Math. Biol. 75, 621–47. (2017).
Google Scholar
Zhang, X., Tang, S., Liu, Q., Cheke, R. A. & Zhu, H. Models to assess the effects of non-identical sex ratio augmentations of Wolbachia-carrying mosquitoes on the control of dengue disease. Math. Biosci. 299, 58–72 (2018).
Google Scholar
Almeida, L., Privat, Y., Strugarek, M. & Vauchelet, N. Optimal releases for population replacement strategies, application to Wolbachia. SIAM Journal on Mathematical Analysis, Society for Industrial and Applied Mathematics 51, 3170–3194 (2019).
Google Scholar
Clements, A. N. The Biology of Mosquitoes: Sensory Reception and Behaviour (Chapman & Hall, 1999).
Downes, J. A. The swarming and mating flight of Diptera. Annu. Rev. Entomol. 14, 271–98. (1969).
Google Scholar
Yuval, B. Mating systems of blood-feeding flies. Annu Rev. Entomol. 51, 413–440 (2006).
Google Scholar
Charlwood, J. D. & Jones, M. D. R. Mating in the mosquito, Anopheles gambiae s.l. Physiol. Entomol. 5, 315–20. (1980).
Google Scholar
Pitts, R. J., Mozuraitis, R., Gauvin-Bialecki, A. & Lemperiere, G. The roles of kairomones, synomones and pheromones in the chemically-mediated behaviour of male mosquitoes. Acta Tropica 132, S26–S34 (2014).
Google Scholar
Hartberg, W. K. Observations on the mating behaviour of Aedes aegypti in nature. Bull. World Health Organ. 45, 847–850 (1971).
Google Scholar
Sawadogo P. S. et al. Swarming behaviour in natural populations of Anopheles gambiae and An. coluzzii: review of 4 years survey in rural areas of sympatry, Burkina Faso (West Africa). Acta Tropica 132, S42-52 https://doi.org/10.1016/j.actatropica.2013.12.011 (2014).
South, A. C. F. Progress in Mosquito Research (Elsevier Science, 2016).
Cator, L. J. & Harrington, L. C. The harmonic convergence of fathers predicts the mating success of sons in Aedes aegypti. Anim. Behav. 82, 627–633 (2011).
Google Scholar
Benelli, G. The best time to have sex: mating behaviour and effect of daylight time on male sexual competitiveness in the Asian tiger mosquito, Aedes albopictus (Diptera: Culicidae). Parasitol. Res. 114, 887–94. (2015).
Google Scholar
Benelli, G., Romano, D., Messing, R. H. & Canale, A. First report of behavioural lateralisation in mosquitoes: right-biased kicking behaviour against males in females of the Asian tiger mosquito, Aedes albopictus. Parasitol. Res. 114, 1613–1617 (2015).
Google Scholar
Cator, L. J. & Zanti, Z. Size, sounds and sex: interactions between body size and harmonic convergence signals determine mating success in Aedes aegypti. Parasites Vectors 9, 622 (2016).
Google Scholar
South, S. H., Steiner, D. & Arnqvist, G. Male mating costs in a polygynous mosquito with ornaments expressed in both sexes. Proc. R. Soc. B 276, 3671–3678 (2009).
Google Scholar
Roth, L. M. A study of mosquito behavior. An experimental laboratory study of the sexual behavior of Aedes aegypti (Linnaeus). Am. Midl. Nat. 40, 265–352 (1948).
Google Scholar
Wishart, G., van Sickle, G. R. & Riordan, D. F. Orientation of the males of Aedes aegypti (L.) (Diptera: Culicidae) to sound. Can. Entomol. 94, 613–26. (1962).
Google Scholar
Belton, P. Attraction of male mosquitoes to sound. J. Am. Mosq. Control Assoc. 10, 297–301 (1994).
Google Scholar
Simões, P. M. V., Ingham, R. A., Gibson, G. & Russell, I. J. A role for acoustic distortion in novel rapid frequency modulation behaviour in free-flying male mosquitoes. J. Exp. Biol. 219, 2039–2047 (2016).
Google Scholar
Simoes, P. M., Gibson, G. & Russell, I. J. Pre-copula acoustic behaviour of males in the malarial mosquitoes Anopheles coluzzii and Anopheles gambiae s.s. does not contribute to reproductive isolation. J. Exp. Biol. 220, 379–85. (2017).
Google Scholar
Gibson, G. & Russell, I. Flying in tune: sexual recognition in mosquitoes. Curr. Biol. 16, 1311–1316 (2006).
Google Scholar
Cator, L. J., Arthur, B. J., Harrington, L. C. & Hoy, R. R. Harmonic convergence in the love songs of the dengue vector mosquito. Science 323, 1077–1079 (2009).
Google Scholar
Warren, B., Gibson, G. & Russell, I. J. Sex recognition through midflight mating duets in culex mosquitoes is mediated by acoustic distortion. Curr. Biol. 19, 485–491 (2009).
Google Scholar
Pennetier, C., Warren, B., Dabiré, K. R., Russell, I. J. & Gibson, G. “Singing on the Wing” as a mechanism for species recognition in the malarial mosquito Anopheles gambiae. Curr. Biol. 20, 131–136 (2010).
Google Scholar
Aldersley, A. & Cator, L. J. Female resistance and harmonic convergence influence male mating success in Aedes aegypti. Sci. Rep. 9, 2145 (2019).
Google Scholar
League, G. P., Baxter, L. L., Wolfner, M. F. & Harrington, L. C. Male accessory gland molecules inhibit harmonic convergence in the mosquito Aedes aegypti. Curr. Biol. 29, R196–r7. (2019).
Google Scholar
Villarreal, S. M. et al. Male contributions during mating increase female survival in the disease vector mosquito Aedes aegypti. J. Insect Physiol. 108, 1–9 (2018).
Google Scholar
Dobson, A. P. & Hudson, P. J. Regulation and stability of a free-living host–parasite system: Trichostrongylus tenuis in Red Grouse. II. Population models. J. Anim. Ecol. 61, 487–498 (1992).
Google Scholar
Hamilton, W. D. & Zuk, M. Heritable true fitness and bright birds: a role for parasites? Science 218, 384–387 (1982).
Google Scholar
Hillyer, J. F., Schmidt, S. L. & Christensen, B. M. Hemocyte-mediated phagocytosis and melanization in the mosquito Armigeres subalbatus following immune challenge by bacteria. Cell Tissue Res. 313, 117–127 (2003).
Google Scholar
Moreno-García, M., Córdoba-Aguilar, A., Condé, R. & Lanz-Mendoza, H. Current immunity markers in insect ecological immunology: assumed trade-offs and methodological issues. Bull. Entomol. Res. 103, 127–139 (2012).
Google Scholar
Schoenle, L. A., Downs, C. J. & Martin L. B. An introduction to ecoimmunology. In Advances in Comparative Immunology (ed. Cooper, E. L.). 901–932 (Springer International Publishing, 2018).
Barthel, A., Staudacher, H., Schmaltz, A., Heckel, D. G. & Groot, A. T. Sex-specific consequences of an induced immune response on reproduction in a moth. BMC Evol. Biol. 15, 282 (2015).
Google Scholar
Hillyer, J. F. & Strand, M. R. Mosquito hemocyte-mediated immune responses. Curr. Opin. Insect Sci. 3, 14–21 (2014).
Google Scholar
Chun, J., Riehle, M. & Paskewitz, S. M. Effect of mosquito age and reproductive status on melanization of sephadex beads in Plasmodium-refractory and -susceptible strains of Anopheles gambiae. J. Invertebr. Pathol. 66, 11–17 (1995).
Google Scholar
Li, J., Tracy, J. W. & Christensen, B. M. Relationship of hemolymph phenol oxidase and mosquito age in Aedes aegypti. J. Invertebr. Pathol. 60, 188–191 (1992).
Google Scholar
Rolff, J. & Siva-Jothy, M. T. Copulation corrupts immunity: a mechanism for a cost of mating in insects. Proc. Natl Acad. Sci. USA 99, 9916–9918 (2002).
Google Scholar
Schwenke, R. A. & Lazzaro, B. P. Juvenile hormone suppresses resistance to infection in mated female Drosophila melanogaster. Curr. Biol. 27, 596–601 (2017).
Google Scholar
Reavey, C. E., Warnock, N. D., Cotter, S. C. & Vogel, H. Trade-offs between personal immunity and reproduction in the burying beetle, Nicrophorus vespilloides. Behav. Ecol. 25, 415–23. (2014).
Google Scholar
Christensen, B. M., Li, J. Y., Chen, C. C. & Nappi, A. J. Melanization immune responses in mosquito vectors. Trends Parasitol. 21, 192–199 (2005).
Google Scholar
Harris, K. L., Christensen, B. M. & Miranpuri, G. S. Comparative studies on the melanization response of male and female mosquitoes against microfilariae. Dev. Comp. Immunol. 10, 305–310 (1986).
Google Scholar
Syed, Z. A., Gupta, V., Arun, M. G., Dhiman, A., Nandy, B. & Prasad, N. G. Absence of reproduction-immunity trade-off in male Drosophila melanogaster evolving under differential sexual selection. BMC Evol. Biol. 20, 13 (2020).
Google Scholar
Schwenke R. A., Lazzaro B. P., Wolfner M. F. Reproduction-immunity trade-offs in insects. Annu. Rev. Entomol. 61, 239–256 (2016).
Schmid-Hempel, P. Evolutionary ecology of insect immune defenses. Annu. Rev. Entomol. 50, 529–551 (2005).
Google Scholar
Armitage, S. A., Thompson, J. J., Rolff, J. & Siva-Jothy, M. T. Examining costs of induced and constitutive immune investment in Tenebrio molitor. J. Evol. Biol. 16, 1038–1044 (2003).
Google Scholar
Schwartz, A. & Koella, J. C. The cost of immunity in the yellow fever mosquito, Aedes aegypti depends on immune activation. J. Evol. Biol. 17, 834–840 (2004).
Google Scholar
Rauw, W. M. Immune response from a resource allocation perspective. Front. Genet. 3, 267 (2012).
Google Scholar
Levashina, E. A., Moita, L. F., Blandin, S., Vriend, G., Lagueux, M. & Kafatos, F. C. Conserved role of a complement-like protein in phagocytosis revealed by dsRNA knockout in cultured cells of the mosquito, Anopheles gambiae. Cell 104, 709–718 (2001).
Google Scholar
Strand, M. R. The insect cellular immune response. Insect Sci. 15, 1–14 (2008).
Google Scholar
Das, S., Dong, Y., Garver, L. & Dimopoulos, G. Specificity of the Innate Immune System: a Closer Look at the Mosquito Pattern-recognition Receptor Repertoire. (Oxford University Press, 2009).
King, J. G. & Hillyer, J. F. Infection-induced interaction between the mosquito circulatory and immune systems. PLoS Pathog. 8, e1003058–e1003058 (2012).
Google Scholar
Murdock, C. C., Paaijmans, K. P., Bell, A. S., King, J. G., Hillyer, J. F. & Read, A. F. et al. Complex effects of temperature on mosquito immune function. Proc. R. Soc. B 279, 3357–3366 (2012).
Google Scholar
Liu, W.-T., Tu, W.-C., Lin, C.-H., Yang, U.-C. & Chen, C.-C. Involvement of cecropin B in the formation of the Aedes aegypti mosquito cuticle. Sci. Rep. 7, 16395 (2017).
Google Scholar
Hillyer, J. F., Schmidt, S. L., Fuchs, J. F., Boyle, J. P. & Christensen, B. M. Age-associated mortality in immune challenged mosquitoes (Aedes aegypti) correlates with a decrease in haemocyte numbers. Cell. Microbiol. 7, 39–51 (2005).
Google Scholar
Coggins, S., Estévez-Lao, T. & Hillyer, J. Increased survivorship following bacterial infection by the mosquito Aedes aegypti as compared to Anopheles gambiae correlates with increased transcriptional induction of antimicrobial peptides. Dev. Comp. Immunol. 37, 390–401 (2012).
Google Scholar
Luckhart, S., Vodovotz, Y., Cui, L. & Rosenberg, R. The mosquito Anopheles stephensi limits malaria parasite development with inducible synthesis of nitric oxide. Proc. Natl Acad. Sci. USA 95, 5700–5705 (1998).
Google Scholar
Graça-Souza, A. V., Maya-Monteiro, C., Paiva-Silva, G. O., Braz, G. R., Paes, M. C. & Sorgine, M. H. et al. Adaptations against heme toxicity in blood-feeding arthropods. Insect Biochem. Mol. Biol. 36, 322–335 (2006).
Google Scholar
Cirimotich, C. M., Ramirez, J. L. & Dimopoulos G. Native microbiota shape insect vector competence for human pathogens. Cell Host Microbe 10, 307–310 (2011).
Sánchez-Vargas, I., Scott, J. C., Poole-Smith, B. K., Franz, A. W., Barbosa-Solomieu, V. & Wilusz, J. et al. Dengue virus type 2 infections of Aedes aegypti are modulated by the mosquito’s RNA interference pathway. PLoS Pathog. 5, e1000299 (2009).
Google Scholar
Souza-Neto, J. A., Sim, S. & Dimopoulos, G. An evolutionary conserved function of the JAK-STAT pathway in anti-dengue defense. Proc. Natl Acad. Sci. 106, 17841–17846 (2009).
Google Scholar
Castillo, J., Brown, M. R. & Strand, M. R. Blood feeding and insulin-like peptide 3 stimulate proliferation of hemocytes in the mosquito Aedes aegypti. PLoS Pathog. 7, e1002274 (2011).
Google Scholar
Bryant, W. B. & Michel, K. Blood feeding induces hemocyte proliferation and activation in the African malaria mosquito, Anopheles gambiae Giles. J. Exp. Biol. 217, 1238–1245 (2014).
Google Scholar
Xi, Z., Ramirez, J. L. & Dimopoulos, G. The Aedes aegypti Toll pathway controls dengue virus infection. PLoS Pathog. 4, e1000098 (2008).
Google Scholar
Bottino-Rojas, V., Talyuli, O. A., Jupatanakul, N., Sim, S., Dimopoulos, G. & Venancio, T. M. et al. Heme signaling impacts global gene expression, immunity and dengue virus infectivity in Aedes aegypti. PLoS ONE 10, e0135985 (2015).
Google Scholar
Oliveira, J. H. M., Talyuli, O. A. C., Goncalves, R. L. S., Paiva-Silva, G. O., Sorgine, M. H. F. & Alvarenga, P. H. et al. Catalase protects Aedes aegypti from oxidative stress and increases midgut infection prevalence of Dengue but not Zika. PLoS Negl. Trop. Dis. 11, e0005525 (2017).
Google Scholar
Bernstein, E., Caudy, A. A., Hammond, S. M. & Hannon, G. J. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409, 363 (2001).
Google Scholar
Elbashir, S. M., Lendeckel, W. & Tuschl, T. RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev. 15, 188–200 (2001).
Google Scholar
Miyoshi, K., Tsukumo, H., Nagami, T., Siomi, H. & Siomi, M. C. Slicer function of Drosophila Argonautes and its involvement in RISC formation. Genes Dev. 19, 2837–2848 (2005).
Google Scholar
Okamura, K., Ishizuka, A., Siomi, H. & Siomi, M. C. Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways. Genes Dev. 18, 1655–1666 (2004).
Google Scholar
Rand, T. A., Ginalski, K., Grishin, N. V. & Wang, X. Biochemical identification of Argonaute 2 as the sole protein required for RNA-induced silencing complex activity. Proc. Natl Acad. Sci. USA 101, 14385–14389 (2004).
Google Scholar
Ramos-Castaneda, J., Gonzalez, C., Jimenez, M. A., Duran, J., Hernandez-Martinez, S. & Rodriguez, M. H. et al. Effect of nitric oxide on dengue virus replication in Aedes aegypti and Anopheles albimanus. Intervirology 51, 335–341 (2008).
Google Scholar
Xiao, X., Liu, Y., Zhang, X., Wang, J., Li, Z. & Pang, X. et al. Complement-related proteins control the flavivirus infection of Aedes aegypti by inducing antimicrobial peptides. PLoS Pathog. 10, e1004027 (2014).
Google Scholar
Waldock, J., Olson, K. E. & Christophides, G. K. Anopheles gambiae antiviral immune response to systemic O’nyong-nyong infection. PLoS Negl. Trop. Dis. 6, e1565 (2012).
Google Scholar
Colpitts, T. M., Cox, J., Vanlandingham, D. L., Feitosa, F. M., Cheng, G. & Kurscheid, S. et al. Alterations in the Aedes aegypti transcriptome during infection with West Nile, dengue and yellow fever Viruses. PLoS Pathog. 7, e1002189 (2011).
Google Scholar
Moon, A. E., Walker A. J. & Goodbourn S. Regulation of transcription of the Aedes albopictus cecropin A1 gene: a role for p38 mitogen-activated protein kinase. Insect Biochem. Mol. Biol. 41, 628–636 (2011).
Jordan, T. X. & Randall, G. Dengue virus activates the AMP kinase-mTOR axis to stimulate a proviral lipophagy. J. Virol. 91, e02020–16 (2017).
Google Scholar
Urbanowski, M. D. & Hobman, T. C. The West Nile virus capsid protein blocks apoptosis through a phosphatidylinositol 3-kinase-dependent mechanism. J. Virol. 87, 872–881 (2013).
Google Scholar
Lazzaro, B. P., Flores, H. A., Lorigan, J. G. & Yourth, C. P. Genotype-by-environment interactions and adaptation to local temperature affect immunity and fecundity in Drosophila melanogaster. PLoS Pathog. 4, e1000025 (2008).
Google Scholar
Jupatanakul, N. et al. Engineered Aedes aegypti JAK/STAT pathway-mediated immunity to dengue virus. PLoS Negl. Trop. Dis. 11, e0005187 (2017).
Martin-Acebes M. A. et al. The composition of West Nile virus lipid envelope unveils a role of sphingolipid metabolism in flavivirus biogenesis. J. Virol. 88, 12041–12054 (2014).
Barletta, A. B., Alves, L. R., Silva, M. C., Sim, S., Dimopoulos, G. & Liechocki, S. et al. Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and dengue virus. Sci. Rep. 6, 19928 (2016).
Google Scholar
Fu, Q., Inankur, B., Yin, J., Striker, R. & Lan, Q. Sterol carrier protein 2, a critical host factor for dengue virus infection, alters the cholesterol distribution in mosquito Aag2 Cells. J. Med. Entomol. 52, 1124–1134 (2015).
Google Scholar
Jupatanakul, N., Sim, S. & Dimopoulos, G. Aedes aegypti ML and Niemann-Pick type C family members are agonists of dengue virus infection. Dev. Comp. Immunol. 43, 1–9 (2014).
Google Scholar
Evans, M. V. et al. Carry-over effects of urban larval environments on the transmission potential of dengue-2 virus. Parasites Vectors 11, 426 (2018).
Salazar, M. I., Richardson, J. H., Sánchez-Vargas, I., Olson, K. E. & Beaty, B. J. Dengue virus type 2: replication and tropisms in orally infected Aedes aegypti mosquitoes. BMC Microbiol. 7, 9 (2007).
Google Scholar
Gloria-Soria, A., Soghigian, J., Kellner, D. & Powell, J. R. Genetic diversity of laboratory strains and implications for research: the case of Aedes aegypti. PLoS Negl. Trop. Dis. 13, e0007930 (2019).
Google Scholar
Souza-Neto, J. A., Powell, J. R. & Bonizzoni, M. Aedes aegypti vector competence studies: a review. Infect. Genet. Evol. 67, 191–209 (2019).
Google Scholar
Franz, A. W. et al. Fitness impact and stability of a transgene conferring resistance to dengue-2 virus following introgression into a genetically diverse Aedes aegypti strain. PLoS Negl. Trop. Dis. 8, e2833 (2014).
Google Scholar
Irvin, N., Hoddle, M. S., O’Brochta, D. A., Carey, B. & Atkinson, P. W. Assessing fitness costs for transgenic Aedes aegypti expressing the GFP marker and transposase genes. Proc. Natl Acad. Sci. USA 101, 891–896 (2004).
Google Scholar
Pompon, J. & Levashina, E. A. A new role of the mosquito complement-like cascade in male fertility in Anopheles gambiae. PLoS Biol. 13, e1002255–e1002255 (2015).
Google Scholar
Mitchell, S. N., Kakani, E. G., South, A., Howell, P. I., Waterhouse, R. M. & Catteruccia, F. Mosquito biology. Evolution of sexual traits influencing vectorial capacity in anopheline mosquitoes. Science 347, 985–988 (2015).
Google Scholar
League G. P. et al. Sexual selection theory meets disease vector control: Testing harmonic convergence as a “good genes” signal in Aedes aegypti mosquitoes. Preprint at bioRxiv https://doi.org/10.1101/2020.10.29.360651 (2020).
Hillyer, J. F. & Estevez-Lao, T. Y. Nitric oxide is an essential component of the hemocyte-mediated mosquito immune response against bacteria. Dev. Comp. Immunol. 34, 141–149 (2010).
Google Scholar
Warburg, A., Shtern, A., Cohen, N. & Dahan, N. Laminin and a Plasmodium ookinete surface protein inhibit melanotic encapsulation of Sephadex beads in the hemocoel of mosquitoes. Microbes Infect. 9, 192–199 (2007).
Google Scholar
Lambrechts, L., Vulule, J. M. & Koella, J. C. Genetic correlation between melanization and antibacterial immune responses in a natural population of the malaria vector Anopheles gambiae. Evolution 58, 2377–2381 (2004).
Google Scholar
Lambrechts, L., Morlais, I., Awono-Ambene, P. H., Cohuet, A., Simard, F. & Jacques, J.-C. et al. Effect of infection by Plasmodium falciparum on the melanization immune response of Anopheles gambiae. Am. J. Tropic. Med. Hyg. 76, 475–480 (2007).
Google Scholar
Boëte, C., Paul, R. E. L. & Koella, J. C. Direct and indirect immunosuppression by a malaria parasite in its mosquito vector. Proc. R. Soc. Lond. Ser. B 271, 1611–1615 (2004).
Google Scholar
Ramakrishnan, M. A. Determination of 50% endpoint titer using a simple formula. World J. Virol. 5, 85–86 (2016).
Google Scholar
Tesla, B., Demakovsky, L. R., Mordecai, E. A., Ryan, S. J., Bonds, M. H. & Ngonghala, C. N. et al. Temperature drives Zika virus transmission: evidence from empirical and mathematical models. Proc. R. Soc. B 285, 20180795 (2018).
Google Scholar
Franz, A. W., Kantor, A. M., Passarelli, A. L. & Clem, R. J. Tissue barriers to arbovirus infection in mosquitoes. Viruses 7, 3741–3767 (2015).
Google Scholar
Lanciotti, R. S., Calisher, C. H., Gubler, D. J., Chang, G. J. & Vorndam, A. V. Rapid detection and typing of dengue viruses from clinical-samples by using reverse transcriptase-polymerase chain-reaction. J. Clin. Microbiol 30, 545–551 (1992).
Google Scholar
RStudio Team. RStudio: Integrated Development Environment for R (RStudio, Inc., 2016).
R. Core Team. R: A Language and Environment for Statistical Computing (R Foundation for Statistical Computing, 2017).
Brooks, M. E., Kristensen, K., van Benthem, K. J., Magnusson, A., Berg, C. W. & Nielsen, A. et al. glmmTMB balances speed and flexibility among packages for zero-inflated generalized linear mixed modeling. R. J. 9, 378–400 (2017).
Google Scholar
Christensen, R. H. B. Ordinal-Regression Models for Ordinal Data. R package version 2015.6-28 (R Foundation for Statistical Computing, 2015).
Bates, D., Mächler, M., Bolker, B. & Walker S. Fitting linear mixed-effects models using lme4. Journal of Statistical Software 67, 48 (2015).
Google Scholar
Bolker, B. R Development Core Team. bbmle: Tools for General Maximum Likelihood Estimation. R package version 1.0.20 (CRAN, 2017).
Hartig, F. DHARMa: Residual Diagnostics for Hierarchical (Multi-Level/Mixed) Regression Models. R package version 0.2.4 ed (CRAN, 2019).
Lenth, R. emmeans: Estimated Marginal Means, aka Least-Squares Means. R package version 1.3.3, ed (CRAN, 2019).
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