Karl D, Letelier R, Tupas L, Dore J, Christian J, Hebel D. The role of nitrogen fixation in biogeochemical cycling in the subtropical North Pacific Ocean. Nature. 1997;388:533–8.
Google Scholar
Jickells TD, Buitenhuis E, Altieri K, Baker AR, Capone D, Duce RA, et al. A reevaluation of the magnitude and impacts of anthropogenic atmospheric nitrogen inputs on the ocean. Glob Biogeochem Cycles. 2017;31:289–305.
Google Scholar
Knapp A. The sensitivity of marine N2 fixation to dissolved inorganic nitrogen. Front Microbiol. 2012;3:374.
Google Scholar
Rees AP, Gilbert JA, Kelly-Gerreyn BA. Nitrogen fixation in the western English Channel (NE Atlantic Ocean). Mar Ecol Prog Ser. 2009;374:7–12.
Google Scholar
Shiozaki T, Nagata T, Ijichi M, Furuya K. Nitrogen fixation and the diazotroph community in the temperate coastal region of the northwestern North Pacific. Biogeosciences. 2015;12:4751–64.
Google Scholar
Tang W, Cerdán-García E, Berthelot H, Polyviou D, Wang S, Baylay A, et al. New insights into the distributions of nitrogen fixation and diazotrophs revealed by high-resolution sensing and sampling methods. ISME J. 2020;14:2514–26.
Google Scholar
Tang W, Wang S, Fonseca-Batista D, Dehairs F, Gifford S, Gonzalez AG, et al. Revisiting the distribution of oceanic N2 fixation and estimating diazotrophic contribution to marine production. Nat Commun. 2019;10:1–10.
Google Scholar
Hamersley M, Turk K, Leinweber A, Gruber N, Zehr J, Gunderson T, Capone D. Nitrogen fixation within the water column associated with two hypoxic basins in the Southern California Bight. Aquat Microb Ecol. 2011;63:193–205.
Google Scholar
Mulholland MR, Bernhardt PW, Blanco-Garcia JL, Mannino A, Hyde K, Mondragon E, et al. Rates of dinitrogen fixation and the abundance of diazotrophs in North American coastal waters between Cape Hatteras and Georges Bank. Limnol Oceanogr. 2012;57:1067–83.
Google Scholar
Mulholland MR, Bernhardt PW, Widner BN, Selden CR, Chappell PD, Clayton S, et al. High rates of N2 fixation in temperate, western North Atlantic coastal waters expands the realm of marine diazotrophy. Glob Biogeochem Cycles. 2019;33:826–40.
Google Scholar
Bentzon-Tilia M, Traving SJ, Mantikci M, Knudsen-Leerbeck H, Hansen JL, Markager S, et al. Significant N2 fixation by heterotrophs, photoheterotrophs and heterocystous cyanobacteria in two temperate estuaries. ISME J. 2015;9:273–85.
Google Scholar
Wen Z, Lin W, Shen R, Hong H, Kao SJ, Shi D. Nitrogen fixation in two coastal upwelling regions of the Taiwan Strait. Sci Rep. 2017;7:1–10.
Google Scholar
Voss M, Bombar D, Loick N, Dippner JW. Riverine influence on nitrogen fixation in the upwelling region off Vietnam, South China Sea. Geophys Res Lett. 2006;33:L07604.
Google Scholar
Shiozaki T, Furuya K, Kodama T, Kitajima S, Takeda S, Takemura T, et al. New estimation of N2 fixation in the western and central Pacific Ocean and its marginal seas. Glob Biogeochem Cycles. 2010;24:GB1015–n/a.
Blais M, Tremblay JÉ, Jungblut AD, Gagnon J, Martin J, Thaler M, et al. Nitrogen fixation and identification of potential diazotrophs in the Canadian Arctic. Glob Biogeochem Cycles. 2012;26:GB3022.
Google Scholar
Shiozaki T, Fujiwara A, Inomura K, Hirose Y, Hashihama F, Harada N. Biological nitrogen fixation detected under Antarctic sea ice. Nat Geosci. 2020;13:729–32.
Google Scholar
Harding K, Turk-Kubo KA, Sipler RE, Mills MM, Bronk DA, Zehr JP. Symbiotic unicellular cyanobacteria fix nitrogen in the Arctic Ocean. Proc Natl Acad Sci USA. 2018;115:13371–5.
Google Scholar
Thompson AW, Foster RA, Krupke A, Carter BJ, Musat N, Vaulot D, et al. Unicellular cyanobacterium symbiotic with a single-celled eukaryotic alga. Science. 2012;337:1546–50.
Google Scholar
Zehr JP, Shilova IN, Farnelid HM, del Carmen Muñoz-MarínCarmen M, Turk-Kubo KA. Unusual marine unicellular symbiosis with the nitrogen-fixing cyanobacterium UCYN-A. Nat Microbiol. 2016;2:16214.
Google Scholar
Zehr JP, Bench SR, Carter BJ, Hewson I, Niazi F, Shi T, et al. Globally distributed uncultivated oceanic N2-fixing cyanobacteria lack oxygenic photosystem II. Science. 2008;322:1110–2.
Google Scholar
Tripp HJ, Bench SR, Turk KA, Foster RA, Desany BA, Niazi F, et al. Metabolic streamlining in an open-ocean nitrogen-fixing cyanobacterium. Nature. 2010;464:90–4.
Google Scholar
Church MJ, Mahaffey C, Letelier RM, Lukas R, Zehr JP, Karl DM. Physical forcing of nitrogen fixation and diazotroph community structure in the North Pacific subtropical gyre. Glob Biogeochem Cycles. 2009;23:GB2020.
Langlois RJ, Hummer D, LaRoche J. Abundances and distributions of the dominant nifH phylotypes in the Northern Atlantic Ocean. Appl Environ Microbiol. 2008;74:1922–31.
Google Scholar
Moisander PH, Beinart RA, Hewson I, White AE, Johnson KS, Carlson CA, et al. Unicellular cyanobacterial distributions broaden the oceanic N2 fixation domain. Science. 2010;327:1512–4.
Google Scholar
Krupke A, Lavik G, Halm H, Fuchs BM, Amann RI, Kuypers MM. Distribution of a consortium between unicellular algae and the N2 fixing cyanobacterium UCYN-A in the North Atlantic Ocean. Environ Microbiol. 2014;16:3153–67.
Google Scholar
Shiozaki T, Bombar D, Riemann L, Hashihama F, Takeda S, Yamaguchi T, et al. Basin scale variability of active diazotrophs and nitrogen fixation in the North Pacific, from the tropics to the subarctic Bering Sea. Glob Biogeochem Cycles 2017;31:996–1009.
Google Scholar
Krupke A, Musat N, Laroche J, Mohr W, Fuchs BM, Amann RI, et al. In situ identification and N2 and C fixation rates of uncultivated cyanobacteria populations. Syst Appl Microbiol. 2013;36:259–71.
Google Scholar
Martínez-Pérez C, Mohr W, Löscher CR, Dekaezemacker J, Littmann S, Yilmaz P, et al. The small unicellular diazotrophic symbiont, UCYN-A, is a key player in the marine nitrogen cycle. Nat Microbiol. 2016;1:16163.
Google Scholar
Mills MM, Turk-Kubo KA, van Dijken GL, Henke BA, Harding K, Wilson ST, et al. Unusual marine cyanobacteria/haptophyte symbiosis relies on N2 fixation even in N-rich environments. ISME J. 2020;14:2395–406.
Google Scholar
Scavotto RE, Dziallas C, Bentzon-Tilia M, Riemann L, Moisander PH. Nitrogen-fixing bacteria associated with copepods in coastal waters of the North Atlantic Ocean. Environ. Microbiol. 2015;17:3754–65.
Google Scholar
Conroy BJ, Steinberg DK, Song B, Kalmbach A, Carpenter EJ, Foster RA. Mesozooplankton graze on cyanobacteria in the amazon river plume and western tropical North Atlantic. Front Microbiol. 2017;8:1436.
Google Scholar
Turk-Kubo KA, Connell P, Caron D, Hogan ME, Farnelid HM, Zehr JP. In situ diazotroph population dynamics under different resource ratios in the North Pacific Subtropical Gyre. Front Microbiol. 2018;9:1616.
Google Scholar
Needham DM, Fuhrman JA. Pronounced daily succession of phytoplankton, archaea and bacteria following a spring bloom. Nat Microbiol. 2016;1:16005.
Google Scholar
Shiozaki T, Fujiwara A, Ijichi M, Harada N, Nishino S, Nishi S, et al. Diazotroph community structure and the role of nitrogen fixation in the nitrogen cycle in the Chukchi Sea (western Arctic Ocean). Limnol Oceanogr. 2018;63:2191–205.
Google Scholar
Sohm JA, Hilton JA, Noble AE, Zehr JP, Saito MA, Webb EA. Nitrogen fixation in the South Atlantic Gyre and the Benguela Upwelling system. Geophys Res Lett. 2011;38:L16608–n/a.
Google Scholar
Moreira-Coello V, Mouriño-Carballido B, Marañón E, Fernández-Carrera A, Bode A, Varela MM. Biological N2 fixation in the upwelling region off NW Iberia: magnitude, relevance, and players. Front Mar Sci. 2017;4:303.
Google Scholar
Cabello AM, Turk-Kubo KA, Hayashi K, Jacobs L, Kudela RM, Zehr JP. Unexpected presence of the nitrogen-fixing symbiotic cyanobacterium UCYN-A in Monterey Bay, California. J Phycol. 2020;56:1521–33.
Google Scholar
Deutsch C, Frenzel H, McWilliams JC, Renault L, Kessouri F, Howard E, et al. Biogeochemical variability in the California Current System. Prog Oceanogr. 2021;196:102565.
Google Scholar
Grasshoff K, Kremling K, Ehrhardt M, editors. Methods of seawater analysis. 3rd ed. Weinheim: Wiley-VCH; 1999.
Welschmeyer NA. Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and phaeopigments. Limnol Oceanogr. 1994;39:1985–92.
Google Scholar
Moisander PH, Beinart RA, Voss M, Zehr JP. Diversity and abundance of diazotrophic microorganisms in the South China Sea during intermonsoon. ISME J. 2008;2:954–67.
Google Scholar
Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, et al. QIIME allows analysis of high-throughput community sequencing data. Nat Methods. 2010;7:335–6.
Google Scholar
Edgar RC. Search and clustering orders of magnitude faster than BLAST. Bioinformatics. 2010;26:2460–1.
Google Scholar
Edgar RC, Haas BJ, Clemente JC, Quince C, Knight R. UCHIME improves sensitivity and speed of chimera detection. Bioinformatics. 2011;27:2194–200.
Google Scholar
Zehr JP, Jenkins BD, Short SM, Steward GF. Nitrogenase gene diversity and microbial community structure: a cross-system comparison. Environ Microbiol. 2003;5:539–54.
Google Scholar
Eren AM, Maignien L, Sul WJ, Murphy LG, Grim SL, Morrison HG, et al. Oligotyping: differentiating between closely related microbial taxa using 16S rRNA gene data. Methods Ecol Evol. 2013;4:1111–9.
Google Scholar
Turk-Kubo KA, Farnelid HM, Shilova IN, Henke B, Zehr JP. Distinct ecological niches of marine symbiotic N2-fixing cyanobacterium Candidatus Atelocyanobacterium thalassa sublineages. J Phycol. 2017;53:451–61.
Google Scholar
Henke BA, Turk-Kubo KA, Bonnet S, Zehr JP. Distributions and abundances of sublineages of the N2-fixing cyanobacterium Candidatus Atelocyanobacterium thalassa (UCYN-A) in the New Caledonian Coral Lagoon. Front Microbiol. 2018;9:554.
Google Scholar
Gradoville MR, Farnelid H, White AE, Turk‐Kubo KA, Stewart B, Ribalet F, et al. Latitudinal constraints on the abundance and activity of the cyanobacterium UCYN‐A and other marine diazotrophs in the North Pacific. Limnol Oceanogr. 2020;65:1858–75.
Google Scholar
McMurdie PJ, Holmes S. phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data. PLoS ONE. 2013;8:e61217.
Google Scholar
Church M, Jenkins B, Karl D, Zehr J. Vertical distributions of nitrogen-fixing phylotypes at Stn ALOHA in the oligotrophic North Pacific Ocean. Aquat Microb Ecol. 2005;38:3–14.
Google Scholar
Thompson A, Carter BJ, Turk-Kubo K, Malfatti F, Azam F, Zehr JP. Genetic diversity of the unicellular nitrogen-fixing cyanobacteria UCYN-A and its prymnesiophyte host. Environ Microbiol. 2014;16:3238–49.
Google Scholar
Foster RA, Subramaniam A, Mahaffey C, Carpenter EJ, Capone DG, Zehr JP. Influence of the Amazon River plume on distributions of free-living and symbiotic cyanobacteria in the western tropical north Atlantic Ocean. Limnol Oceanogr. 2007;52:517–32.
Google Scholar
Goebel NL, Turk KA, Achilles KM, Paerl R, Hewson I, Morrison AE, et al. Abundance and distribution of major groups of diazotrophic cyanobacteria and their potential contribution to N2 fixation in the tropical Atlantic Ocean. Environ Microbiol. 2010;12:3272–89.
Google Scholar
Farnelid H, Turk-Kubo K, Munoz-Marin MD, Zehr JP. New insights into the ecology of the globally significant uncultured nitrogen-fixing symbiont UCYN-A. Aquat Microb Ecol. 2016;77:125–38.
Google Scholar
Mohr W, Grosskopf T, Wallace DWR, LaRoche J. Methodological underestimation of oceanic nitrogen fixation rates. PLoS ONE. 2010;5:e12583.
Google Scholar
Montoya JP, Voss M, Kahler P, Capone DG. A simple, high-precision, high-sensitivity tracer assay for N2 fixation. Appl Environ Microbiol. 1996;62:986–93.
Google Scholar
Gradoville MR, Bombar D, Crump BC, Letelier RM, Zehr JP, White AE. Diversity and activity of nitrogen-fixing communities across ocean basins. Limnol Oceanogr. 2017;62:1895–909.
Google Scholar
White AE, Granger J, Selden C, Gradoville MR, Potts L, Bourbonnais A, et al. A critical review of the 15N2 tracer method to measure diazotrophic production in pelagic ecosystems. Limnol Oceanogr Methods. 2020;18:129–47.
Google Scholar
Cornejo-Castillo FM, Cabello AM, Salazar G, Sánchez-Baracaldo P, Lima-Mendez G, Hingamp P, et al. Cyanobacterial symbionts diverged in the late Cretaceous towards lineage-specific nitrogen fixation factories in single-celled phytoplankton. Nat Commun. 2016;7:11071.
Google Scholar
Cabello AM, Cornejo-Castillo FM, Raho N, Blasco D, Vidal M, Audic S, et al. Global distribution and vertical patterns of a prymnesiophyte-cyanobacteria obligate symbiosis. ISME J. 2016;10:693–706.
Google Scholar
Polerecky L, Adam B, Milucka J, Musat N, Vagner T, Kuypers MM. Look@ NanoSIMS–a tool for the analysis of nanoSIMS data in environmental microbiology. Environ Microbiol. 2012;14:1009–23.
Google Scholar
Krupke A, Mohr W, LaRoche J, Fuchs BM, Amann RI, Kuypers MM. The effect of nutrients on carbon and nitrogen fixation by the UCYN-A-haptophyte symbiosis. ISME J. 2015;9:1635–47.
Google Scholar
Meyer NR, Fortney J, Dekas AE. NanoSIMS sample preparation decreases isotope enrichment: magnitude, variability and implications for single-cell rates of microbial activity. Environ Microbiol. 2020;23:81–98.
Google Scholar
Durazo R. Seasonality of the transitional region of the California Current System off Baja California. J Geophys Res Oceans. 2015;120:1173–96.
Google Scholar
Bakun A. Coastal upwelling indices, west coast of North America, 1946–71.
Redfield AC. On the proportions of organic derivatives in sea water and their relation to the composition of plankton. Vol. 1. Liverpool: University Press of Liverpool; 1934. p. 176–92.
Bograd SJ, Schroeder ID, Jacox MG. A water mass history of the Southern California current system. Geophys. Res. Lett. 2019;46:6690–8.
Google Scholar
Langlois R, Großkopf T, Mills M, Takeda S, LaRoche J. Widespread distribution and expression of gamma A (UMB), an uncultured, diazotrophic, γ-proteobacterial nifH phylotype. PLoS ONE. 2015;10:e0128912.
Google Scholar
Dekaezemacker J, Bonnet S, Grosso O, Moutin T, Bressac M, Capone DG. Evidence of active dinitrogen fixation in surface waters of the eastern tropical South Pacific during El Niño and La Niña events and evaluation of its potential nutrient controls. Glob Biogeochem Cycles 2013;27:768–79.
Google Scholar
Chen M, Lu Y, Jiao N, Tian J, Kao SJ, Zhang Y. Biogeographic drivers of diazotrophs in the western Pacific Ocean. Limnol Oceanogr. 2019;64:1403–21.
Google Scholar
Turk KA, Rees AP, Zehr JP, Pereira N, Swift P, Shelley R, et al. Nitrogen fixation and nitrogenase (nifH) expression in tropical waters of the eastern North Atlantic. ISME J. 2011;5:1201–12.
Google Scholar
White AE, Foster RA, Benitez-Nelson CR, Masqué P, Verdeny E, Popp BN, et al. Nitrogen fixation in the Gulf of California and the Eastern Tropical North Pacific. Prog Oceanogr. 2013;109:1–17.
Google Scholar
Selden CR, Mulholland MR, Bernhardt PW, Widner B, Macías‐Tapia A, Ji Q, et al. Dinitrogen fixation across physico-chemical gradients of the eastern tropical North Pacific oxygen deficient zone. Glob Biogeochem Cycles. 2019;33:1187–202.
Google Scholar
Sohm JA, Webb EA, Capone DG. Emerging patterns of marine nitrogen fixation. Nat Rev Microbiol. 2011;9:499–508.
Google Scholar
Carlucci A, Bowes PM. Production of vitamin B12, thiamine, and biotin by phytoplankton. J Phycol. 1970;6:351–7.
Google Scholar
Gledhill M, Buck KN. The organic complexation of iron in the marine environment: a review. Front Microbiol. 2012;3:69.
Google Scholar
Biddanda B, Benner R. Carbon, nitrogen, and carbohydrate fluxes during the production of particulate and dissolved organic matter by marine phytoplankton. Limnol Oceanogr. 1997;42:506–18.
Google Scholar
Hernández de la Torre B, Gaxiola Castro G, Álvarez Borrego S, Gallegos García A, Aguirre Gómez R. New organic carbon in front of the Baja California Peninsula: time series and climatology. Hidrobiológica. 2015;25:74–85.
Xiu P, Chai F, Curchitser EN, Castruccio FS. Future changes in coastal upwelling ecosystems with global warming: the case of the California Current System. Sci. Rep. 2018;8:2866.
Google Scholar
Kimor B, Reid F, Jordan J. An unusual occurrence of Hemiaulus membranaceus Cleve (Bacillariophyceae) with Richelia intracelluaris Schmidt (Cyanophyceae) off the coast of Southern California. Phycologia. 1978;17:162–6.
Google Scholar
White AE, Prahl FG, Letelier RM, Popp BN. Summer surface waters in the Gulf of California: Prime habitat for biological N2 fixation. Glob Biogeochem Cycles. 2007;21:GB2017–n/a.
Pyle AE, Johnson AM, Villareal TA. Isolation, growth, and nitrogen fixation rates of the Hemiaulus-Richelia (diatom-cyanobacterium) symbiosis in culture. PeerJ. 2020;8:e10115.
Google Scholar
Foster RA, Kuypers MM, Vagner T, Paerl RW, Musat N, Zehr JP. Nitrogen fixation and transfer in open ocean diatom–cyanobacterial symbioses. ISME J. 2011;5:1484–93.
Google Scholar
Caputo A, Nylander JAA, Foster RA. The genetic diversity and evolution of diatom-diazotroph associations highlights traits favoring symbiont integration. FEMS Microbiol Lett. 2019;366:fny297.
Google Scholar
Thompson AR. State of the California Current 2017–18: still not quite normal in the north and getting interesting in the south. California cooperative oceanic fisheries investigations, Data report. 2018.
Larkin AA, Moreno AR, Fagan AJ, Fowlds A, Ruiz A, Martiny AC. Persistent El Nino driven shifts in marine cyanobacteria populations. PLoS ONE. 2020;15:e0238405.
Google Scholar
Hagino K, Takano Y, Horiguchi T. Pseudo-cryptic speciation in Braarudosphaera bigelowii (Gran and Braarud) Deflandre. Mar Micropaleontol. 2009;72:210–21.
Google Scholar
Selden CR, Chappell PD, Clayton S, Macías‐Tapia A, Bernhardt PW, Mulholland MR. A coastal N2 fixation hotspot at the Cape Hatteras front: elucidating spatial heterogeneity in diazotroph activity via supervised machine learning. Limnol Oceanogr. 2021;66:1832–49.
Google Scholar
Wang S, Tang W, Delage E, Gifford S, Whitby H, González AG, et al. Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic. Sci Rep. 2021;11:5508.
Google Scholar
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