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The population genomic structure of green turtles (Chelonia mydas) suggests a warm-water corridor for tropical marine fauna between the Atlantic and Indian oceans during the last interglacial

  • Alfaro-Nunez A, Bertelsen MF, Bojesen AM, Rasmussen I, Zepeda-Mendoza L, Olsen MT et al. (2014) Global distribution of Chelonid fibropapilloma-associated herpesvirus among clinically healthy sea turtles. BMC Evol Biol 14:206

    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Ali OA, O’Rourke SM, Amish SJ, Meek MH, Luikart G, Jeffres C et al. (2016) RAD capture (Rapture): flexible and efficient sequence-based genotyping. Genetics 202:389–400

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • Amos B, Hoelzel AR (1991). Long-term preservation of whale skin for DNA analysis. Rep Int Whal Comm Spec Issue 13:99–103

  • Baird NA, Etter PD, Atwood TS, Currey MC, Shiver AL, Lewis ZA et al. (2008) Rapid SNP discovery and genetic mapping using sequenced RAD markers. PLoS ONE 3:e3376–8

    PubMed 
    PubMed Central 
    Article 
    CAS 

    Google Scholar 

  • Ball RM, Neigel JE, Avise JC (1990) Gene genealogies within the organismal pedigrees of random-mating populations. Evolution 44:360–370

    PubMed 
    PubMed Central 

    Google Scholar 

  • Beerli P (2006) Comparison of Bayesian and maximum-likelihood inference of population genetic parameters. Bioinformatics 22:341–345

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • Beerli P, Palczewski M (2010) Unified framework to evaluate panmixia and migration direction among multiple sampling locations. Genetics 185:313–326

    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Bell CDL, Parsons J, Austin TJ, Broderick AC, Ebanks-Petrie G, Godley BJ (2005) Some of them came home: the Cayman Turtle Farm headstarting project for the green turtle Chelonia mydas. Oryx 39:137–148

    Article 

    Google Scholar 

  • Bhatia G, Patterson N, Sankararaman S, Price AL (2013) Estimating and interpreting FST: The impact of rare variants. Genome Res 23:1514–1521

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Bintanja R, van de Wal RSW, Oerlemans J (2005) Modelled atmospheric temperatures and global sea levels over the past million years. Nature 437:125–128

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Bjorndal KA, Bolten AB, Chaloupka MY (2000) Green turtle somatic growth model: evidence for density dependence. Ecol Appl 10:269–282

    Google Scholar 

  • Bjorndal KA, Bolten AB, Chaloupka M, Saba VS, Bellini C, Marcovaldi MAG et al. (2017) Ecological regime shift drives declining growth rates of sea turtles throughout the West Atlantic. Glob Change Biol 23:4556–4568

    Article 

    Google Scholar 

  • Bourjea J, Lapegue S, Gagnevin L, Broderick D, Mortimer JA, Ciccione S et al. (2007) Phylogeography of the green turtle, Chelonia mydas, in the Southwest Indian Ocean. Mol Ecol 16:175–186

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Bowen BW, Abreu-Grobois FA, Balazs GH, Kamezaki N, Limpus CJ, Ferl RJ (1995) Trans-Pacific migrations of the loggerhead turtle (Caretta caretta) demonstrated with mitochondrial DNA markers. Proc Natl Acad Sci 92:3731–3734

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Bowen BW, Clark AM, Abreu-Grobois FA, Chaves A, Reichart HA, Ferl RJ (1997) Global phylogeography of the ridley sea turtles (Lepidochelys spp.) as inferred from mitochondrial DNA sequences. Genetica 101:179–189

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Bowen BW, Gaither MR, DiBattista JD, Iacchei M, Andrews KR, Grant WS et al. (2016) Comparative phylogeography of the ocean planet. Proc Natl Acad Sci 113:7962–7969

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Boyle MC, FitzSimmons NN, Limpus CJ, Kelez S, Velez-Zuazo X, Waycott M (2009) Evidence for transoceanic migrations by loggerhead sea turtles in the southern Pacific Ocean. Proc R Soc B 276:1993–1999

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Bradshaw PJ, Broderick AC, Carreras C, Fuller W, Snape RTE, Wright LI et al. (2018) Defining conservation units with enhanced molecular tools to reveal fine scale structuring among Mediterranean green turtle rookeries. Biol Conserv 222:253–260

    Article 

    Google Scholar 

  • Carr AF, Carr MH, Meylan AB (1978). The ecology and migrations of sea turtles, 7. The West Caribbean green turtle colony. Bull Am Mus Nat Hist 162:1–46

  • Catchen JM, Amores A, Hohenlohe P, Cresko W, Postlethwait JH (2011) Stacks: building and genotyping loci de novo from short-read sequences. G3 Genes Genomes Genet 1:171–182

    CAS 

    Google Scholar 

  • Clark PU, Dyke AS, Shakun JD, Carlson AE, Clark J, Wohlfarth B et al. (2009) The last glacial maximum. Science 325:710–714

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Coates AG, Jackson JBC, Collins LS, Cronin TM, Dowsett HJ, Bybell LM et al. (1992) Closure of the Isthmus of Panama: the near-shore marine record of Costa Rica and western Panama. GSA Bull 104:814–828

    Article 

    Google Scholar 

  • Dahl-Jensen D, Albert MR, Aldahan A, Azuma N, Balslev-Clausen D, Baumgartner M et al. (2013) Eemian interglacial reconstructed from a Greenland folded ice core. Nature 493:489–494

    CAS 
    Article 

    Google Scholar 

  • Danecek P, Auton A, Abecasis G, Albers CA, Banks E, DePristo MA et al. (2011) The variant call format and VCFtools. Bioinformatics 27:2156–2158

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Domingues RR, Hilsdorf AWS, Shivji MM, Hazin FVH, Gadig OBF (2018) Effects of the Pleistocene on the mitochondrial population genetic structure and demographic history of the silky shark (Carcharhinus falciformis) in the western Atlantic Ocean. Rev Fish Biol Fish 28:213–227

    Article 

    Google Scholar 

  • Dray S, Dufour A-B (2007) The ade4 package: Implementing the duality diagram for ecologists. J Stat Softw 22:1–20.

    Article 

    Google Scholar 

  • Duncan KM, Martin AP, Bowen BW, Couet HGD (2006) Global phylogeography of the scalloped hammerhead shark (Sphyrna lewini). Mol Ecol 15:2239–2251

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Edwards S, Beerli P (2000) Perspective: gene divergence, population divergence, and the variance in coalescence time in phylogeographic studies. Evolution 54:1839–1854

    CAS 
    PubMed 

    Google Scholar 

  • Encalada SE, Lahanas PN, Bjorndal KA, Bolten AB, Miyamoto MM, Bowen BW (1996) Phylogeography and population structure of the Atlantic and Mediterranean green turtle Chelonia mydas: a mitochondrial DNA control region sequence assessment. Mol Ecol 5:473–483

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • Falush D, Stephens M, Pritchard JK (2003) Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics 164:1567–1587

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Felsenstein J (2006) Accuracy of coalescent likelihood estimates: do we need more sites, more sequences, or more loci? Mol Biol Evol 23:691–700

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • Felsenstein J, Churchill GA (1996) A Hidden Markov Model approach to variation among sites in rate of evolution. Mol Biol Evol 13:93–104

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • FitzSimmons NN, Limpus CJ, Norman JA, Goldizen AR, Miller JD, Moritz C (1997) Philopatry of male marine turtles inferred from mitochondrial DNA markers. Proc Natl Acad Sci 94:8912–8917

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Francis RM (2017) pophelper: an R package and web app to analyse and visualize population structure. Mol Ecol Resour 17:27–32

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • Gaither MR, Bernal MA, Fernandez-Silva I, Mwale M, Jones SA, Rocha C et al. (2015) Two deep evolutionary lineages in the circumtropical glasseye Heteropriacanthus cruentatus (Teleostei, Priacanthidae) with admixture in the south-western Indian Ocean. J Fish Biol 87:715–727

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • Gaither MR, Bowen BW, Bordenave T-R, Rocha LA, Newman SJ, Gomez JA et al. (2011). Phylogeography of the reef fish Cephalopholis argus (Epinephelidae) indicates Pleistocene isolation across the Indo-Pacific barrier with contemporary overlap in the coral triangle. BMC Evol Biol 11:189

  • Gnirke A, Melnikov A, Maguire J, Rogov P, LeProust EM, Brockman W et al. (2009) Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing. Nat Biotechnol 27:182–189

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Goshe LR, Avens L, Scharf FS, Southwood AL (2010) Estimation of age at maturation and growth of Atlantic green turtles (Chelonia mydas) using skeletochronology. Mar Biol 157:1725–1740

    Article 

    Google Scholar 

  • Green RE, Braun EL, Armstrong J, Earl D, Nguyen N, Hickey G et al. (2014) Three crocodilian genomes reveal ancestral patterns of evolution among archosaurs. Science 346:1254449

    PubMed 
    PubMed Central 
    Article 
    CAS 

    Google Scholar 

  • Hamann M, Godfrey MH, Seminoff JA, Arthur K, Barata PCR, Bjorndal KA et al. (2010) Global research priorities for sea turtles: informing management and conservation in the 21st century. Endanger Species Res 11:245–269

    Article 

    Google Scholar 

  • Hewitt G (2000) The genetic legacy of the Quaternary ice ages. Nature 405:907–913

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • Ho SYW, Phillips MJ, Cooper A, Drummond AJ (2005) Time dependency of molecular rate estimates and systematic overestimation of recent divergence times. Mol Biol Evol 22:1561–1568

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • Hudson RR, Slatkin M, Maddison WP (1992) Estimation of levels of gene flow from DNA sequence data. Genetics 132:583–589

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Jensen MP, Dalleau M, Gaspar P, Lalire M, Jean C, Ciccione S et al. (2020) Seascape genetics and the spatial ecology of juvenile green turtles. Genes 11:278

    CAS 
    PubMed Central 
    Article 

    Google Scholar 

  • Jensen MP, FitzSimmons NN, Bourjea J, Hamabata T, Reece J, Dutton PH (2019) The evolutionary history and global phylogeography of the green turtle (Chelonia mydas). J Biogeogr 46:1–11.

    Article 

    Google Scholar 

  • Jombart T, Devillard S, Balloux F (2010) Discriminant analysis of principal components: a new method for the analysis of genetically structured populations. BMC Genet 11:94

    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Kalinowski ST (2011) The computer program STRUCTURE does not reliably identify the main genetic clusters within species: simulations and implications for human population structure. Heredity 106:625–632

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • Karl SA, Bowen BW, Avise JC (1992) Global population genetic structure and male-mediated gene flow in the green turtle (Chelonia mydas): RFLP analyses of anonymous nuclear loci. Genetics 131:163–173

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Kelleher J, Etheridge AM, McVean G (2016) Efficient coalescent simulation and genealogical analysis for large sample sizes. PLOS Comput Biol 12:e1004842

    PubMed 
    PubMed Central 
    Article 
    CAS 

    Google Scholar 

  • Kiessling W, Simpson C, Beck B, Mewis H, Pandolfi JM (2012) Equatorial decline of reef corals during the last Pleistocene interglacial. Proc Natl Acad Sci 109:21378–21383

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Komoroske LM, Miller MR, O’Rourke SM, Stewart KR, Jensen MP, Dutton PH (2019) A versatile Rapture (RAD-Capture) platform for genotyping marine turtles. Mol Ecol Resour 19:497–511

    PubMed 
    Article 

    Google Scholar 

  • Kong A, Frigge ML, Masson G, Besenbacher S, Sulem P, Magnusson G et al. (2012) Rate of de novo mutations and the importance of father’s age to disease risk. Nature 488:471–475

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Kukla GJ, Bender ML, de Beaulieu J-L, Bond G, Broecker WS, Cleveringa P et al. (2002) Last interglacial climates. Quat Res 58:2–13

    Article 

    Google Scholar 

  • Lahanas PN, Bjorndal KA, Bolten AB, Encalada SE, Miyamoto MM, Valverde RA et al. (1998) Genetic composition of a green turtle (Chelonia mydas) feeding ground population: evidence for multiple origins. Mar Biol 130:345–352

    Article 

    Google Scholar 

  • Langmead B, Trapnell C, Pop M, Salzberg SL (2009) Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol 10:R25

    PubMed 
    PubMed Central 
    Article 
    CAS 

    Google Scholar 

  • Latch EK, Dharmarajan G, Glaubitz JC, Rhodes Jr OE (2006) Relative performance of Bayesian clustering software for inferring population substructure and individual assignment at low levels of population differentiation. Conserv Genet 7:295–302

    Article 

    Google Scholar 

  • Lessios HA (2008) The great American schism: divergence of marine organisms after the rise of the Central American Isthmus. Annu Rev Ecol Evol Syst 39:63–91

    Article 

    Google Scholar 

  • Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N et al. (2009) The Sequence Alignment/Map format and SAMtools. Bioinformatics 25:2078–2079

    PubMed 
    PubMed Central 
    Article 
    CAS 

    Google Scholar 

  • Linck EB, Battey CJ (2019) Minor allele frequency thresholds strongly affect population structure inference with genomic datasets. Mol Ecol Resour 19:639–647

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Ludt WB, Rocha LA (2015) Shifting seas: the impacts of Pleistocene sea-level fluctuations on the evolution of tropical marine taxa. J Biogeogr 42:25–38

    Article 

    Google Scholar 

  • Malinsky M, Trucchi E, Lawson DJ, Falush D (2018) RADpainter and fineRADstructure: population inference from RADseq data. Mol Biol Evol 35:1284–1290

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Maruki T, Lynch M (2017) Genotype calling from population-genomic sequencing data. G3 Genes Genomes Genet 7:1393–1404

    CAS 

    Google Scholar 

  • Meylan AB, Bowen BW, Avise JC (1990) A genetic test of the natal homing versus social facilitation models for green turtle migration. Science 248:724–727

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Miles A, Murillo R, Ralph P, Harding N, Pisupati R, Rae S et al. (2017). cggh/scikit-allel: v1.3.2. Zenodo

  • Monzón-Argüello C, López-Jurado LF, Rico C, Marco A, López P, Hays GC et al. (2010) Evidence from genetic and Lagrangian drifter data for transatlantic transport of small juvenile green turtles. J Biogeogr 37:1752–1766

    Article 

    Google Scholar 

  • Naro-Maciel E, Reid BN, Alter SE, Amato G, Bjorndal KA, Bolten AB et al. (2014) From refugia to rookeries: phylogeography of Atlantic green turtles. J Exp Mar Biol Ecol 461:306–316

    Article 

    Google Scholar 

  • Patrício AR, Formia A, Barbosa C, Broderick AC, Bruford M, Carreras C et al. (2017) Dispersal of green turtles from Africa’s largest rookery assessed through genetic markers. Mar Ecol Prog Ser 569:215–225

    Article 

    Google Scholar 

  • Peeters FJC, Acheson R, Brummer G-JA, De Ruijter WPM, Schneider RR, Ganssen GM et al. (2004) Vigorous exchange between the Indian and Atlantic oceans at the end of the past five glacial periods. Nature 430:661–665

    CAS 
    PubMed 
    Article 

    Google Scholar 

  • Penven P, Lutjeharms JRE, Marchesiello P, Roy C, Weeks SJ (2001) Generation of cyclonic eddies by the Agulhas Current in the lee of the Agulhas Bank. Geophys Res Lett 28:1055–1058

    Article 

    Google Scholar 

  • Peterson BK, Weber JN, Kay EH, Fisher HS, Hoekstra HE (2012) Double digest RADseq: an inexpensive method for de novo SNP discovery and genotyping in model and non-model species. PLoS ONE 7:e37135

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Pillans B, Chappell J, Naish TR (1998) A review of the Milankovitch climatic beat: template for Plio–Pleistocene sea-level changes and sequence stratigraphy. Sediment Geol 122:5–21

    CAS 
    Article 

    Google Scholar 

  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • R Core Team (2021) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria

    Google Scholar 

  • Reece JS, Castoe TA, Parkinson CL (2005) Historical perspectives on population genetics and conservation of three marine turtle species. Conserv Genet 6:235–251

    Article 

    Google Scholar 

  • Reid BN, Naro‐Maciel E, Hahn AT, FitzSimmons NN, Gehara M (2019) Geography best explains global patterns of genetic diversity and post-glacial co-expansion in marine turtles. Mol Ecol 28:3358–3370

    PubMed 
    PubMed Central 

    Google Scholar 

  • Roberts MA, Schwartz TS, Karl SA (2004) Global population genetic structure and male-mediated gene flow in the green sea turtle (Chelonia mydas): analysis of microsatellite loci. Genetics 166:1857–1870

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Rocha LA (2003) Patterns of distribution and processes of speciation in Brazilian reef fishes. J Biogeogr 30:1161–1171

    Article 

    Google Scholar 

  • Rocha LA, Robertson DR, Rocha CR, Van Tassell JL, Craig MT, Bowen BW (2005) Recent invasion of the tropical Atlantic by an Indo-Pacific coral reef fish. Mol Ecol 14:3921–3928

    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • Scally A, Durbin R (2012) Revising the human mutation rate: implications for understanding human evolution. Nat Rev Genet 13:745–753

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • Seminoff JA (2004). Chelonia mydas. IUCN Red List Threat Species 2004: e.T4615A11037468

  • Seminoff JA, Allen CD, Balazs GH, Dutton PH, Eguchi T, Haas HL et al. (2015). Status review of the green turtle (Chelonia mydas) under the U.S. Endangered Species Act. NOAA Technical Memorandum, NOAA-NMFS-SWFSC-539. 571pp

  • Strasburg JL, Rieseberg LH (2010) How robust are ‘isolation with migration’ analyses to violations of the im model? A simulation study. Mol Biol Evol 27:297–310

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • Taberlet P, Fumagalli L, Wust‐Saucy A-G, Cosson J-F (1998) Comparative phylogeography and postglacial colonization routes in Europe. Mol Ecol 7:453–464

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • Teske PR, Von Der Heyden S, McQuaid CD, Barker NP (2011) A review of marine phylogeography in southern. Afr South Afr J Sci 107:43–53

    Google Scholar 

  • Van der Zee JP, Christianen MJA, Nava M, Velez-Zuazo X, Hao W, Bérubé M et al. (2019) Population recovery changes population composition at a major southern Caribbean juvenile developmental habitat for the green turtle, Chelonia mydas. Sci Rep 9:14392

  • Wallace BP, DiMatteo AD, Hurley BJ, Finkbeiner EM, Bolten AB, Chaloupka MY et al. (2010) Regional management units for marine turtles: a novel framework for prioritizing conservation and research across multiple scales. PLoS ONE 5:e15465

    PubMed 
    PubMed Central 
    Article 
    CAS 

    Google Scholar 

  • Wang J (2017) The computer program structure for assigning individuals to populations: easy to use but easier to misuse. Mol Ecol Resour 17:981–990

    CAS 
    PubMed 
    Article 
    PubMed Central 

    Google Scholar 

  • Wang Z, Pascual-Anaya J, Zadissa A, Li W, Niimura Y, Huang Z et al. (2013) The draft genomes of soft-shell turtle and green sea turtle yield insights into the development and evolution of the turtle-specific body plan. Nat Genet 45:701–706

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Waples RS (1998) Separating the wheat from the chaff: patterns of genetic differentiation in high gene flow species. J Hered 89:438–450

    Article 

    Google Scholar 

  • Weir BS, Cockerham CC (1984) Estimating F-Statistics for the Analysis of Population Structure. Evolution 38:1358–1370

    CAS 
    PubMed 

    Google Scholar 

  • Willing E-M, Dreyer C, van Oosterhout C (2012) Estimates of genetic differentiation measured by FST do not necessarily require large sample sizes when using many SNP markers. PLoS ONE 7:e42649–7

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar 

  • Wilson AC, Cann RL, Carr SM, George M, Gyllensten UB, Helm-Bychowski KM et al. (1985) Mitochondrial DNA and two perspectives on evolutionary genetics. Biol J Linn Soc 26:375–400

    Article 

    Google Scholar 

  • Woodruff DS (2010) Biogeography and conservation in Southeast Asia: how 2.7 million years of repeated environmental fluctuations affect today’s patterns and the future of the remaining refugial-phase biodiversity. Biodivers Conserv 19:919–941

    Article 

    Google Scholar 

  • Wright S (1951) The genetical structure of populations. Ann Eugen 15:323–354

    CAS 
    PubMed 
    Article 

    Google Scholar 


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