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Characterizing phenotypic diversity in marine populations of the threespine stickleback

  • Bell, M. A. & Foster, S. A. The Evolutionary Biology of the Threespine Stickleback (Oxford University Press, 1994).

    Google Scholar 

  • Seebacher, F., Webster, M. M., James, R. S., Tallis, J. & Ward, A. J. W. Morphological differences between habitats are associated with physiological and behavioural trade-offs in stickleback (Gasterosteus aculeatus). R. Soc. Open Sci. 3, 160316 (2016).

    ADS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Bolnick, D. I. et al. Phenotype-dependent native habitat preference facilitates divergence between parapatric lake and stream stickleback. Evolution 63, 2004–2016 (2009).

    PubMed 

    Google Scholar 

  • Svanbäck, R. & Schluter, D. Niche specialization influences adaptive phenotypic plasticity in the threespine stickleback. Am. Nat. 180, 50–59 (2012).

    PubMed 

    Google Scholar 

  • Caldecutt, W. J. & Adams, D. C. Morphometrics of trophic osteology in the threespine stickleback, Gasterosteus aculeatus. Copeia 1998, 827–838 (1998).

    Google Scholar 

  • Yershov, P. & Sukhotin, A. Age and growth of marine three-spined stickleback in the White Sea 50 years after a population collapse. Polar Biol. 38, 1813–1823 (2015).

    Google Scholar 

  • Dorgham, A. S. et al. Morphological variation of threespine stickleback (Gasterosteus aculeatus) on different stages of spawning period. Proc. KarRC RAS 59–73 (2018). https://doi.org/10.17076/them819.

  • DeFaveri, J. & Merilä, J. Evidence for adaptive phenotypic differentiation in Baltic Sea sticklebacks. J. Evol. Biol. 26, 1700–1715 (2013).

    CAS 
    PubMed 

    Google Scholar 

  • Shaw, K. A., Scotti, M. L. & Foster, S. A. Ancestral plasticity and the evolutionary diversification of courtship behaviour in threespine sticklebacks. Anim. Behav. 73, 415–422 (2007).

    Google Scholar 

  • McGee, M. D., Schluter, D. & Wainwright, P. C. Functional basis of ecological divergence in sympatric stickleback. BMC Evol. Biol. 13, 277 (2013).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Berner, D., Grandchamp, A.-C. & Hendry, A. P. Variable progress toward ecological speciation in parapatry: Stickleback across eight lake-stream transitions. Evolution 63, 1740–1753 (2009).

    PubMed 

    Google Scholar 

  • Walker, J. A. Ecological morphology of lacustrine threespine stickleback Gasterosteus aculeatus L. (Gasterosteidae) body shape. Biol. J. Linn. Soc. 61, 3–50 (1997).

    Google Scholar 

  • Hagen, D. W. & Gilbertson, L. G. Geographic variation and environmental selection in Gasterosteus aculeatus L. in the Pacific Northwest America. Evolution 26, 32–51 (1972).

    CAS 
    PubMed 

    Google Scholar 

  • Smith, C., Zięba, G., Spence, R., Klepaker, T. & Przybylski, M. Three-spined stickleback armour predicted by body size, minimum winter temperature and pH. J. Zool. 311, 13–22 (2020).

    Google Scholar 

  • Aguirre, W. E. & Bell, M. A. Twenty years of body shape evolution in a threespine stickleback population adapting to a lake environment: Stickleback body shape evolution. Biol. J. Linn. Soc. 105, 817–831 (2012).

    Google Scholar 

  • Lavin, P. A. & McPhail, J. D. The evolution of freshwater diversity in the threespine stickleback (Gasterosteus aculeatus): Site-specific differentiation of trophic morphology. Can. J. Zool. 63, 2632–2638 (1985).

    Google Scholar 

  • Matthews, B., Marchinko, K. B., Bolnick, D. I. & Mazumder, A. Specialization of trophic position and habitat use by sticklebacks in an adaptive radiation. Ecology 91, 1025–1034 (2010).

    PubMed 

    Google Scholar 

  • Lefébure, R., Larsson, S. & Byström, P. A temperature-dependent growth model for the three-spined stickleback Gasterosteus aculeatus. J. Fish Biol. 79, 1815–1827 (2011).

    PubMed 

    Google Scholar 

  • Foster, S. A. Inference of evolutionary pattern: Diversionary displays of three-spined sticklebacks. Behav. Ecol. 5, 114–121 (1992).

    Google Scholar 

  • Taylor, E. B. & McPhail, J. D. Evolutionary history of an adaptive radiation in species pairs of threespine sticklebacks (Gasterosteus): Insights from mitochondrial DNA. Biol. J. Linn. Soc. 66, 271–291 (1999).

    Google Scholar 

  • Hohenlohe, P. A., Bassham, S., Currey, M. & Cresko, W. A. Extensive linkage disequilibrium and parallel adaptive divergence across threespine stickleback genomes. Phil. Trans. R. Soc. B 367, 395–408 (2012).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Walker, J. A. & Bell, M. A. Net evolutionary trajectories of body shape evolution within a microgeographic radiation of threespine sticklebacks (Gasterosteus aculeatus). J. Zool. 252, 293–302 (2000).

    Google Scholar 

  • Kristjánsson, B. K., Skúlason, S. & Noakes, D. L. G. Rapid divergence in a recently isolated population of threespine stickleback (Gasterosteus aculeatus L.). Evol. Ecol. Res. 4, 659–672 (2002).

    Google Scholar 

  • Wund, M. A., Baker, J. A., Clancy, B., Golub, J. L. & Foster, S. A. A test of the “flexible stem” model of evolution: Ancestral plasticity, genetic accommodation, and morphological divergence in the threespine stickleback radiation. Am. Nat. 172, 449–462 (2008).

    PubMed 

    Google Scholar 

  • Arif, S., Aguirre, W. E. & Bell, M. A. Evolutionary diversification of opercle shape in Cook Inlet threespine stickleback. Biol. J. Linn. Soc. 97, 832–844 (2009).

    Google Scholar 

  • Terekhanova, N. V. et al. Fast evolution from precast bricks: Genomics of young freshwater populations of threespine stickleback Gasterosteus aculeatus. PLoS Genet. 10, e1004696 (2014).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Miller, S. E., Roesti, M. & Schluter, D. A single interacting species leads to widespread parallel evolution of the stickleback genome. Curr. Biol. 29, 530–537 (2019).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Ab Ghani, N. I., Herczeg, G. & Merilä, J. Effects of perceived predation risk and social environment on the development of three-spined stickleback (Gasterosteus aculeatus) morphology. Biol. J. Linn. Soc. 118, 520–535 (2016).

    Google Scholar 

  • DeFaveri, J. & Merilä, J. Local adaptation to salinity in the three-spined stickleback?. J. Evol. Biol. 27, 290–302 (2014).

    CAS 
    PubMed 

    Google Scholar 

  • Jakubavičiūtė, E., De Blick, Y., Dainys, J., Ložys, L. & Olsson, J. Morphological divergence of three-spined stickleback in the Baltic Sea—Implications for stock identification. Fish. Res. 204, 305–315 (2018).

    Google Scholar 

  • Yanos, C. L. et al. Predator biomass and vegetation influence the coastal distribution of threespine stickleback morphotypes. Ecol. Evol. 00, 1–12 (2021).

    Google Scholar 

  • Fang, B., Merilä, J., Ribeiro, F., Alexandre, C. M. & Momigliano, P. Worldwide phylogeny of three-spined sticklebacks. Mol. Phylogenet. Evol. 127, 613–625 (2018).

    PubMed 

    Google Scholar 

  • Ortí, G., Bell, M. A., Reimchen, T. E. & Meyer, A. Global survey of mitochondrial DNA sequences in the threespine sticklebacks: Evidence for recent migrations. Evolution 48, 608–622 (1994).

    PubMed 

    Google Scholar 

  • Mäkinen, H. S. & Merilä, J. Mitochondrial DNA phylogeography of the three-spined stickleback (Gasterosteus aculeatus) in Europe: Evidence for multiple glacial refugia. Mol. Phylogenet. Evol. 46, 167–182 (2008).

    PubMed 

    Google Scholar 

  • Thomson, R. E. Oceanography of the British Columbia Coast (Department of Fisheries and Oceans, 1981).

    Google Scholar 

  • Emmett, R. et al. Geographic signatures of North American west coast estuaries. Estuaries 23, 765 (2000).

    CAS 

    Google Scholar 

  • Dallimore, A. & Jmieff, D. Canadian west coast fjords and inlets. Geol. Soc. Spec. Pub. 344, 143–162 (2010).

    Google Scholar 

  • Schoch, G. C., Albert, D. M. & Shanley, C. S. An estuarine habitat classification for a complex fjordal island archipelago. Estuaries Coasts 37, 160–176 (2014).

    Google Scholar 

  • Rudnick, D. L. & Ferrari, R. Compensation of horizontal temperature and salinity gradients in the ocean mixed layer. Science 283, 526–529 (1999).

    ADS 
    CAS 
    PubMed 

    Google Scholar 

  • Barrett, R. D. H., Rogers, S. M. & Schluter, D. Environment specific pleiotropy facilitates divergence at the Ectodysplasin locus in threespine stickleback. Evolution 63, 2831–2837 (2009).

    PubMed 

    Google Scholar 

  • McCairns, R. J. S. & Bernatchez, L. Plasticity and heritability of morphological variation within and between parapatric stickleback demes. J. Evol. Biol. 25, 1097–1112 (2012).

    CAS 
    PubMed 

    Google Scholar 

  • Webster, M. M., Atton, N., Hart, P. J. B. & Ward, A. J. W. Habitat-specific morphological variation among threespine sticklebacks (Gasterosteus aculeatus) within a drainage basin. PLoS ONE 6, e21060 (2011).

    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Spoljaric, M. A. & Reimchen, T. E. 10 000 years later: evolution of body shape in Haida Gwaii three-spined stickleback. J. Fish. Biol. 70, 1484–1503 (2007).

    Google Scholar 

  • Spoljaric, M. A. & Reimchen, T. E. Habitat-dependent reduction of sexual dimorphism in geometric body shape of Haida Gwaii threespine stickleback. Biol. J. Linn. Soc. 95, 505–516 (2008).

    Google Scholar 

  • Spoljaric, M. A. & Reimchen, T. E. Habitat-specific trends in ontogeny of body shape in stickleback from coastal archipelago: Potential for rapid shifts in colonizing populations. J. Morphol. 272, 590–597 (2011).

    CAS 
    PubMed 

    Google Scholar 

  • Morris, M. R. J. et al. Gene expression plasticity evolves in response to colonization of freshwater lakes in threespine stickleback. Mol. Ecol. 23, 3226–3240 (2014).

    PubMed 

    Google Scholar 

  • Ramler, D., Mitteroecker, P., Shama, L. N. S., Wegner, K. M. & Ahnelt, H. Nonlinear effects of temperature on body form and developmental canalization in the threespine stickleback. J. Evol. Biol. 27, 497–507 (2014).

    CAS 
    PubMed 

    Google Scholar 

  • Mazzarella, A. B., Voje, K. L., Hansson, T. H., Taugbøl, A. & Fischer, B. Strong and parallel salinity-induced phenotypic plasticity in one generation of threespine stickleback. J. Evol. Biol. 28, 667–677 (2015).

    CAS 
    PubMed 

    Google Scholar 

  • Leinonen, T., Cano, J. M., Mäkinen, H. & Merilä, J. Contrasting patterns of body shape and neutral genetic divergence in marine and lake populations of threespine sticklebacks. J. Evol. Biol. 19, 1803–1812 (2006).

    CAS 
    PubMed 

    Google Scholar 

  • Schluter, D., Marchinko, K. B., Barrett, R. D. H. & Rogers, S. M. Natural selection and the genetics of adaptation in threespine stickleback. Phil. Trans. R. Soc. B 365, 2479–2486 (2010).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Rogers, S. M. et al. Genetic signature of adaptive peak shift in threespine stickleback. Evolution 66, 2439–2450 (2012).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Jamniczky, H. A., Barry, T. N. & Rogers, S. M. Eco-evo-devo in the study of adaptive divergence: Examples from threespine stickleback (Gasterosteus aculeatus). Integr. Comp. Biol. 55, 166–178 (2015).

    PubMed 

    Google Scholar 

  • Gow, J. L., Rogers, S. M., Jackson, M. & Schluter, D. Ecological predictions lead to the discovery of a benthic–limnetic sympatric species pair of threespine stickleback in Little Quarry Lake, British Columbia. Can. J. Zool. 86, 564–571 (2008).

    Google Scholar 

  • McPhail, J. D. Genetic evidence for a species pair in Enos Lake, British Columbia. Can. J. Zool. 62, 1402–1408 (1984).

    Google Scholar 

  • McPhail, J. D. Ecology and evolution of sympatric sticklebacks (Gasterosteus): Origin of the species pairs. Can. J. Zool. 71, 515–523 (1993).

    Google Scholar 

  • Kimmel, C. B., Aguirre, W., Ullmann, B., Currey, M. & Cresko, W. Allometric change accompanies opercular shape evolution in Alaskan threespine sticklebacks. Behaviour 145, 669–691 (2008).

    Google Scholar 

  • Wootton, R. J. A Functional Biology of Sticklebacks (Croom Helm, 1984).

    Google Scholar 

  • Kitano, J., Mori, S. & Peichel, C. L. Sexual dimorphism in the external morphology of the threespine stickleback (Gasterosteus aculeatus). Copeia 2, 336–349 (2007).

    Google Scholar 

  • Aguirre, W. E., Ellis, K. E., Kusenda, M. & Bell, M. A. Phenotypic variation and sexual dimorphism in anadromous threespine stickleback: Implications for postglacial adaptive radiation. Biol. J. Linn. Soc. 95, 465–478 (2008).

    Google Scholar 

  • Davenne, E. & Masson, D. Water properties in the Straits of Georgia and Juan de Fuca. 41 http://www.pac.dfo-mpo.gc.ca/sci/osap/projects/straitofgeorgia/JdFG_e.pdf (2001).

  • Irvine, J. R. & Crawford, W. R. State of the Ocean Report for the Pacific North Coast Integrated Management Area (PNCIMA). 51 (2011).

  • DFO. Data from British Columbia (BC) Lighthouses. Department of Fisheries and Oceans https://www.dfo-mpo.gc.ca/science/data-donnees/lightstations-phares/index-eng.html (2020).

  • Palumbi, S. R. Genetic divergence, reproductive isolation, and marine speciation. Annu. Rev. Ecol. Evol. Syst. 25, 547–572 (1994).

    Google Scholar 

  • Griffin, D. A. & LeBlond, P. H. Estuary/ocean exchange controlled by spring-neap tidal mixing. Estuar. Coast Shelf. Sci. 30, 275–297 (1990).

    ADS 

    Google Scholar 

  • Vaz, N., Dias, J. M., Leitão, P. & Martins, I. Horizontal patterns of water temperature and salinity in an estuarine tidal channel: Ria de Aveiro. Ocean Dyn. 55, 416–429 (2005).

    ADS 

    Google Scholar 

  • Rybkina, E. V., Ivanova, T. S., Ivanov, M. V., Kucheryavyy, A. V. & Lajus, D. L. Habitat preference of three-spined stickleback juveniles in experimental conditions and in wild eelgrass. J. Mar. Biol. Ass. UK 97, 1437–1445 (2017).

    Google Scholar 

  • Flynn, S., Cadrin, C. & Filatow, D. Estuaries in British Columbia. 6 (2006).

  • Kelly, J. R., Proctor, H. & Volpe, J. P. Intertidal community structure differs significantly between substrates dominated by native eelgrass (Zostera marina L.) and adjacent to the introduced oyster Crassostrea gigas (Thunberg) in British Columbia, Canada. Hydrobiologia 596, 57–66 (2008).

    Google Scholar 

  • Fagherazzi, S. et al. Ecogeomorphology of Salt Marshes. In The Ecogeomorphology of Tidal Marshes (eds Blum, L. K. & Marani, M.) 182–200 (American Geophysical Union, 2004).

    Google Scholar 

  • Campbell, A. Vegetation-environment relationships and plant community classification and ordination in British Columbia coastal salt marshes. Master’s Thesis. (University of British Columbia, 1986).

  • Kjerfve, B. Comparative oceanography of coastal lagoons. in Estuarine Variability (ed. Wolfe, D. A.) 63–81 (Academic Press, 1986). https://doi.org/10.1016/B978-0-12-761890-6.50009-5.

  • Barnes, R. S. K. & de Villiers, C. J. Animal abundance and food availability in coastal lagoons and intertidal marine sediments. J. Mar. Biol. Ass. UK 80, 193–202 (2000).

    Google Scholar 

  • Saimoto, R. K. Life history of marine stickleback in Oyster Lagoon, British Columbia. Master’s Thesis. (University of British Columbia, 1993).

  • King, R. W. The threespine stickleback adaptive radiation: Salinity, plasticity, and the important of ancestry. Doctoral Dissertation. (Clark University, 2016).

  • Ahnelt, H. Imprecise naming: the anadromous and the sea spawning threespine stickleback should be discriminated by names. Biologia 73, 389–392 (2018).

    Google Scholar 

  • Morris, M. R. J., Bowles, E., Allen, B. E., Jamniczky, H. A. & Rogers, S. M. Contemporary ancestor? Adaptive divergence from standing genetic variation in Pacific marine threespine stickleback. BMC Evol. Biol. 18, 113 (2018).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Kim, S.-Y., Costa, M. M., Esteve-Codina, A. & Velando, A. Transcriptional mechanisms underlying life-history responses to climate change in the three-spined stickleback. Evol. Appl. 10, 718–730 (2017).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Sambrook, R. J. Interactions between threespine stickleback (Gasterosteus aculeatus linnæus) and juvenile Chinook salmon (Oncorhynchus tshawytscha Walbaum) in an estuarine marsh. Master’s Thesis. (University of British Columbia, 1990). https://doi.org/10.14288/1.0098704.

  • Jakubavičiūtė, E., Bergström, U., Eklöf, J. S., Haenel, Q. & Bourlat, S. J. DNA metabarcoding reveals diverse diet of the three-spined stickleback in a coastal ecosystem. PLoS ONE 12, e0186929 (2017).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Kennedy, G. J. A. & Strange, C. D. The distribution of salmonids in upland streams in relation to depth and gradient. J. Fish Biol. 20, 579–591 (1982).

    Google Scholar 

  • Macdonald, J. S., Birtwell, I. K. & Kruzynski, G. M. Food and habitat utilization by juvenile salmonids in the Campbell River estuary. Can. J. Fish. Aquat. Sci. 44, 1233–1246 (1987).

    Google Scholar 

  • Everest, F. H. & Chapman, D. W. Habitat selection and spatial interaction by juvenile chinook salmon and steelhead trout in two Idaho streams. J. Fish. Res. Bd. Can. 29, 91–100 (2011).

    Google Scholar 

  • McPhail, J. D. Speciation and the evolution of reproductive isolation in the sticklebacks (Gasterosteus) of south-western British Columbia. In The Evolutionary Biology of the Threespine Stickleback (eds Bell, M. A. & Foster, S. A.) 399–471 (Oxford University Press, 1994).

    Google Scholar 

  • Kimmel, C. B. et al. Independent axes of genetic variation and parallel evolutionary divergence of opercle bone shape in threespine stickleback. Evolution 66, 419–434 (2012).

    PubMed 

    Google Scholar 

  • Østbye, K. et al. The temporal window of ecological adaptation in postglacial lakes: A comparison of head morphology, trophic position and habitat use in Norwegian threespine stickleback populations. BMC Evol. Biol. 16, 102 (2016).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Aguirre, W. E. & Akinpelu, O. Sexual dimorphism of head morphology in three-spined stickleback Gasterosteus aculeatus. J. Fish Biol. 77, 802–821 (2010).

    CAS 
    PubMed 

    Google Scholar 

  • Reimchen, T. E. & Nosil, P. Variable predation regimes predict the evolution of sexual dimorphism in a population of threespine stickleback. Evolution 58, 1274 (2004).

    CAS 
    PubMed 

    Google Scholar 

  • Pistore, A. Ontogeny of population-specific phenotypic variation in the threespine stickleback. Master’s Thesis. (University of Calgary, 2018).

  • Yurtseva, A. O. et al. Aging three-spined sticklebacks Gasterosteus aculeatus: Comparison of estimates from three structures. J. Fish Biol. 95, 802–811 (2019).

    PubMed 

    Google Scholar 

  • Picard, P. Jr., Dodson, J. J. & FitzGerald, G. J. Habitat segregation among the age groups of Gasterosteus aculeatus (Pisces: Gasterosteidae) in the middle St. Lawrence estuary, Canada. Can. J. Zool. 68, 1202–1208 (1990).

    Google Scholar 

  • Reimchen, T. E., Bergström, C. A. & Nosil, P. Natural selection and the adaptive radiation of Haida Gwaii stickleback. Evol. Ecol. Res. 15, 241–269 (2013).

    Google Scholar 

  • Raeymaekers, J. A. M., Delaire, L. & Hendry, A. P. Genetically based differences in nest characteristics between lake, inlet, and hybrid threespine stickleback from the Misty system, British Columbia, Cananda. Evol. Ecol. Res. 11, 905–919 (2009).

    Google Scholar 

  • Di Poi, C., Lacasse, J., Rogers, S. M. & Aubin-Horth, N. Evolution of stress reactivity in stickleback. Evol. Ecol. Res. 17, 395–405 (2016).

    Google Scholar 

  • Weber, J. N., Bradburd, G. S., Stuart, Y. E., Stutz, W. E. & Bolnick, D. I. Partitioning the effects of isolation by distance, environment, and physical barriers on genomic divergence between parapatric threespine stickleback. Evolution 71, 342–356 (2017).

    PubMed 

    Google Scholar 

  • Rohlf, F. J. Package: tpsUtil, tps file utility program. Version 1. 61. Department of Ecology and Evolution, State University of New York at Stony Brook, Stony Brook, NY. (2015).

  • Rohlf, F. J. Package: tpsDig, digitize landmarks and outlines. Version 2. 05. Department of Ecology and Evolution, State University of New York at Stony Brook, Stony Brook, NY. (2005).

  • Adams, D. C., Collyer, M. L. & Kaliontzopoupou, A. Geomorph: Software for geometric morphometric analysis (2020).

  • Zelditch, M. L., Swiderski, D. L. & Sheets, H. D. Geometric Morphometrics for Biologists: A Primer (Elsevier Academic Press, 2012).

    MATH 

    Google Scholar 

  • Galipaud, M., Gillingham, M. A. F., David, M. & Dechaume-Moncharmont, F.-X. Ecologists overestimate the importance of predictor variables in model averaging: A plea for cautious interpretations. Methods Ecol. Evol. 5, 983–991 (2014).

    Google Scholar 

  • Scheipl, F., Greven, H. & Kuechenhoff, H. Size and power of tests for a zero random effect variance or polynomial regression in additive and linear mixed models. Comput. Stat. Data Anal. 52, 3283–3299 (2008).

    MathSciNet 
    MATH 

    Google Scholar 

  • Robinson, J. James Robinson’s functions. Version 0. 0. 0. 1. Retrieved from https://rdrr.io/github/jpwrobinson/funk/. (2019).

  • Bartoń, K. R Package: MuMIn: Multi-model inference. Version 1. 43. 17. Retrieved from https://CRAN.R-project.org/package=MuMIn. (2020).

  • Frank, A. Diagnosing collinearity in mixed models from lme4 R package, vif.mer function [R script]. Retrieved from https://raw.githubusercontent.com/aufrank/R-hacks/master/mer-utils.R. GitHub https://raw.githubusercontent.com/aufrank/R-hacks/master/mer-utils.R. (2011).

  • Lakens, D. Calculating and reporting effect sizes to facilitate cumulative science: a practical primer for t-tests and ANOVAs. Front. Psychol. https://doi.org/10.3389/fpsyg.2013.00863 (2013).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Kilkenny, C., Browne, W. J., Cuthill, I. C., Emerson, M. & Altman, D. G. Improving bioscience research reporting: The ARRIVE guidelines for reporting animal research. PLoS Biol. 8, e1000412 (2010).

    PubMed 
    PubMed Central 

    Google Scholar 


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