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Neolithic dental calculi provide evidence for environmental proxies and consumption of wild edible fruits and herbs in central Apennines

  • Asevedo, L. et al. Palynological analysis of dental calculus from Pleistocene proboscideans of southern Brazil: a new approach for paleodiet and paleoenvironmental reconstructions. Palaeogeogr. Palaeoclimatol. Palaeoecol. 540, 109523 (2020).

    Article 

    Google Scholar 

  • Cristiani, E. et al. Wild cereal grain consumption among Early Holocene foragers of the Balkans predates the arrival of agriculture. Elife 10, e72976 (2021).

    Article 
    CAS 

    Google Scholar 

  • Nava, A. et al. Multipronged dental analyses reveal dietary differences in last foragers and first farmers at Grotta Continenza, central Italy (15,500–7000 BP). Sci. Rep. 11, 1–14 (2021).

    Article 

    Google Scholar 

  • Ottoni, C. et al. Tracking the transition to agriculture in Southern Europe through ancient DNA analysis of dental calculus. Proc. Natl. Acad. Sci. USA 118, e2102116118 (2021).

    Article 
    CAS 

    Google Scholar 

  • Cammidge, T. S., Kooyman, B. & Theodor, J. M. Diet reconstructions for end-Pleistocene Mammut americanum and Mammuthus based on comparative analysis of mesowear, microwear, and dental calculus in modern Loxodonta africana. Palaeogeogr. Palaeoclimatol. Palaeoecol. 538, 109403 (2020).

    Article 

    Google Scholar 

  • de Oliveira, K. et al. From oral pathology to feeding ecology: the first dental calculus paleodiet study of a South American native megamammal. J. S. Am. Earth Sci. 109, 103281 (2021).

    Article 

    Google Scholar 

  • Mothé, D. et al. The micro from mega: dental calculus description and the first record of fossilized oral bacteria from an extinct proboscidean. Int. J. Paleopathol. 33, 55–60 (2021).

    Article 

    Google Scholar 

  • Eglinton, G. & Logan, G. A. Molecular preservation. Philosophical Transactions of the Royal Society of London. Philos. Trans. R. Soc. Lond., B, Biol. Sci. 333, 315–328 (1991).

    CAS 

    Google Scholar 

  • Romanowski, G., Lorenz, M. G. & Wackernagel, W. Adsorption of plasmid DNA to mineral surfaces and protection against Dnase I. Appl. Environ. Microbiol. 57, 1057–1061 (1991).

    Article 
    CAS 

    Google Scholar 

  • Milanesi, C. et al. Ultrastructural study of archaeological Vitis vinifera L. seeds using rapid-freeze fixation and substitution. Tissue Cell 41, 443–447 (2009).

    Article 
    CAS 

    Google Scholar 

  • Power, R. C., Salazar-García, D. C., Wittig, R. M., Freiberg, M., & Henry, A. G. Dental calculus evidence of Taï Forest chimpanzee plant consumption and life history transitions. Sci. Rep. 5, 15161 (2015).

  • Goude, G. et al. A multidisciplinary approach to Neolithic life reconstruction. J. Archaeol. Method Theory 26, 537–560 (2019).

    Article 

    Google Scholar 

  • Farrer, A. G. et al. Effectiveness of decontamination protocols when analyzing ancient DNA preserved in dental calculus. Sci. Rep. 11, 1–14 (2021).

    Article 

    Google Scholar 

  • Weyrich, L. S., Dobney, K. & Cooper, A. Ancient DNA analysis of dental calculus. J. Hum. Evol. 79, 119–124 (2015).

    Article 

    Google Scholar 

  • Ozga, A. T. et al. Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus. Am. J. Phys. Anthropol. 160, 220–228 (2016).

    Article 

    Google Scholar 

  • Mann, A. E. et al. Do I have something in my teeth? The trouble with genetic analyses of diet from archaeological dental calculus. Quat. Int. https://doi.org/10.1016/j.quaint.2020.11.019 (2020).

  • Wright, S. L., Dobney, K. & Weyrich, L. S. Advancing and refining archaeological dental calculus research using multiomic frameworks. Sci. Technol. Archaeol. Res. 7, 13–30 (2021).

    Google Scholar 

  • Sawafuji, R., Saso, A., Suda, W., Hattori, M. & Ueda, S. Ancient DNA analysis of food remains in human dental calculus from the Edo period, Japan. PLoS One 15, e0226654 (2020).

    Article 
    CAS 

    Google Scholar 

  • Weyrich, L. S. et al. Neanderthal behaviour, diet, and disease inferred from ancient DNA in dental calculus. Nature 544, 357–361 (2017).

    Article 
    CAS 

    Google Scholar 

  • Ottoni, C. et al. Metagenomic analysis of dental calculus in ancient Egyptian baboons. Sci. Rep. 9, 1–10 (2019).

    Article 

    Google Scholar 

  • Hollingsworth, P. M., Graham, S. W. & Little, D. P. Choosing and using a Plant DNA barcode. PLoS One 6, 1–13 (2011).

    Article 

    Google Scholar 

  • Gismondi, A., Fanali, F., Labarga, J. M. M., Caiola, M. G. & Canini, A. Crocus sativus L. genomics and different DNA barcode applications. Plant Syst. Evol. 299, 1859–1863 (2013).

    Article 
    CAS 

    Google Scholar 

  • ICSN. The international code for starch nomenclature, accessed 15 September 2021; http://fossilfarm.org/ICSN/Code.html (2011).

  • Gismondi, A. et al. Starch granules: a data collection of 40 food species. Plant Biosyst. 153, 273–279 (2019).

    Article 

    Google Scholar 

  • Henry, A. G., Brooks, A. S. & Piperno, D. R. Plant foods and the dietary ecology of Neanderthals and early modern humans. J. Hum. Evol. 69, 44–54 (2014).

    Article 

    Google Scholar 

  • PalDat. A palynological database (2000 onwards), accessed 19 January 2022; https://www.paldat.org/ (2019).

  • Berglund, B. E. & Ralska-Jasiewiczowa, M. Pollen analysis and pollen diagrams. In Handbook of Holocene Palaeoecology and Palaeohydrology (ed. Berglund, B. E.) 455–484 (Wiley, 1986).

  • Faegri, K. & Iversen, J. Textbook of Pollen analysis, 4th edn (eds Faegri, K. et al.) (John Wiley and Sons-Chichester, 1989).

  • Grímsson, F. et al. Fagaceae pollen from the early Cenozoic of West Greenland: revisiting Engler’s and Chaney’s Arcto-Tertiary hypotheses. Plant Syst. Evol. 301, 809–832 (2015).

    Article 

    Google Scholar 

  • Denk, T. & Tekleva, M. V. Pollen morphology and ultrastructure of Quercus with focus on Group Ilex (= Quercus Subgenus Heterobalanus (Oerst.) Menitsky): Implications for oak systematics and evolution. Grana 53, 255–282 (2014).

    Article 

    Google Scholar 

  • Grímsson, F. & Zetter, R. Combined LM and SEM study of the middle Miocene (Sarmatian) palynofora from the Lavanttal Basin, Austria: Part II. Pinophyta (Cupressaceae, Pinaceae and Sciadopityaceae). Grana 50, 262–310 (2011).

    Article 

    Google Scholar 

  • Mohanty, R. P., Buchheim, M. A., Portman, R. & Levetin, E. Molecular and ultrastructural detection of plastids in Juniperus (Cupressaceae) pollen. Phytologia 98, 298–310 (2016).

    Google Scholar 

  • Martin, A. C. & Harvey, W. J. The Global Pollen Project: a new tool for pollen identifcation and the dissemination of physical reference collections. Methods Ecol. Evol. 8, 892–897 (2017).

    Article 

    Google Scholar 

  • Maciejewska-Rutkowska, I., Bocianowski, J. & Wrońska-Pilarek, D. Pollen morphology and variability of Polish native species from genus Salix L. PLoS One 16, e0243993 (2021).

    Article 
    CAS 

    Google Scholar 

  • Abreu, I., Costa, I., Oliveira, M., Cunha, M. & De Castro, R. Ultrastructure and germination of Vitis vinifera cv. Loureiro pollen. Protoplasma 228, 131–135 (2006).

    Article 
    CAS 

    Google Scholar 

  • Nagels, A. et al. Palynological diversity and major evolutionary trends in Cyperaceae. Plant Syst. Evol. 277, 117 (2009).

    Article 

    Google Scholar 

  • El Ghazali, G. E. Pollen morphological studies in Amaranthaceae s. lat. (incl. Chenopodiaceae) and their taxonomic significance: a review. Grana 61, 1–7 (2022).

    Article 

    Google Scholar 

  • Petraco, N., & Kubic, T. Color Atlas and Manual of Microscopy for Criminalists, Chemists, and Conservators (Boca Raton-CRC Press, 2003).

  • D’Agostino, A. et al. Environmental implications and evidence of natural products from dental calculi of a Neolithic–Chalcolithic community (central Italy). Sci. Rep. 11, 1–13 (2021).

    Article 

    Google Scholar 

  • Frangiote-Pallone, S. & de Souza, L. A. Pappus and cypsela ontogeny in Asteraceae: structural considerations of the tribal category. Rev. Mex. Biodivers. 85, 62–77 (2014).

    Article 

    Google Scholar 

  • Eglinton, G., Gonzalez, A. G., Hamilton, R. J. & Raphael, R. A. Hydrocarbon constituents of the wax coatings of plant leaves: a taxonomic survey. Phytochemistry 1, 89–102 (1962).

    Article 
    CAS 

    Google Scholar 

  • Buckley, S. A., Stott, A. W. & Evershed, R. P. Studies of organic residues from ancient Egyptian mummies using high temperature-gas chromatography-mass spectrometry and sequential thermal desorption-gas chromatography-mass spectrometry and pyrolysis-gas chromatography-mass spectrometry. Analyst 124, 443–452 (1999).

    Article 
    CAS 

    Google Scholar 

  • Hardy, K. et al. Neanderthal medics? Evidence for food, cooking, and medicinal plants entrapped in dental calculus. Naturwissenschaften 99, 617–626 (2012).

    Article 
    CAS 

    Google Scholar 

  • Luong, S., Tocheri, M. W., Sutikna, T., Saptomo, E. W. & Roberts, R. G. Incorporating terpenes, monoterpenoids and alkanes into multiresidue organic biomarker analysis of archaeological stone artefacts from Liang Bua (Flores, Indonesia). J. Archaeol. Sci. Rep. 19, 189–199 (2018).

    Google Scholar 

  • Luong, S. et al. Combined organic biomarker and use-wear analyses of stone artefacts from Liang Bua, Flores, Indonesia. Sci. Rep. 9, 1–17 (2019).

    Article 
    CAS 

    Google Scholar 

  • Dabney, J., Meyer, M. & Pääbo, S. Ancient DNA damage. Cold Spring Harb. Perspect. Biol. 5, a012567 (2013).

    Article 

    Google Scholar 

  • Mann, A. E. et al. Differential preservation of endogenous human and microbial DNA in dental calculus and dentin. Sci. Rep. 8, 1–15 (2018).

    Article 

    Google Scholar 

  • Horrocks, M., Nieuwoudt, M. K., Kinaston, R., Buckley, H. & Bedford, S. Microfossil and Fourier Transform InfraRed analyses of Lapita and post-Lapita human dental calculus from Vanuatu, Southwest Pacific. J. R. Soc. N. Z. 44, 17–33 (2014).

    Article 

    Google Scholar 

  • Radini, A., Nikita, E., Buckley, S., Copeland, L. & Hardy, K. Beyond food: the multiple pathways for inclusion of materials into ancient dental calculus. Am. J. Phys. Anthropol. 162, 71–83 (2017).

    Article 

    Google Scholar 

  • Henry, A. G. Other microparticles: volcanic glass, minerals, insect remains, feathers, and other plant parts. In Handbook for the Analysis of Micro-Particles in Archaeological Samples 289–295 (Springer, Cham, 2020).

  • MacKenzie, L., Speller, C. F., Holst, M., Keefe, K., & Radini, A. Dental calculus in the industrial age: human dental calculus in the Post-Medieval period, a case study from industrial Manchester. Quat. Int. https://doi.org/10.1016/j.quaint.2021.09.020 (2021).

  • Radini, A., & Nikita, E. Beyond dirty teeth: Integrating dental calculus studies with osteoarchaeological parameters. Quat. Int. https://doi.org/10.1016/j.quaint.2022.03.003 (2022).

  • Dobney, K. & Brothwell, D. A scanning electron microscope study of archaeological dental calculus. In Scanning Electron Microscopy in Archaeology BAR International Series (ed. & Olsen S), vol. 452, pp. 372–385 (Oxford, UK: BAR, 1988).

  • Henry, A. G. & Piperno, D. R. Using plant microfossils from dental calculus to recover human diet: a case study from Tell al-Raqā’i, Syria. J. Archaeol. Sci. 35, 1943–1950 (2008).

    Article 

    Google Scholar 

  • Wesolowski, V., de Souza, S. M. F. M., Reinhard, K. J. & Ceccantini, G. Evaluating microfossil content of dental calculus from Brazilian sambaquis. J. Archaeol. Sci. 37, 1326–1338 (2010).

    Article 

    Google Scholar 

  • González-Guarda, E. et al. Multiproxy evidence for leaf-browsing and closed habitats in extinct proboscideans (Mammalia, Proboscidea) from Central Chile. Proc. Natl. Acad. Sci. USA 115, 9258–9263 (2018).

    Article 

    Google Scholar 

  • Radley, J. A. Starch and its Derivatives (Chapman and Hall, London, 1968).

  • Power, R. C., Salazar-García, D. C., Wittig, R. M. & Henry, A. G. Assessing use and suitability of scanning electron microscopy in the analysis of micro remains in dental calculus. J. Archaeol. Sci. 49, 160–169 (2014).

    Article 
    CAS 

    Google Scholar 

  • Rottoli, M. & Castiglioni, E. Prehistory of plant growing and collecting in northern Italy, based on seed remains from the early Neolithic to the Chalcolithic (c. 5600–2100 cal BC). Veg. Hist. Archaeobot. 18, 91–103 (2009).

    Article 

    Google Scholar 

  • Fiorentino, G. et al. Climate changes and human–environment interactions in the Apulia region of southeastern Italy during the Neolithic period. Holocene 23, 1297–1316 (2013).

    Article 

    Google Scholar 

  • Rottoli, M., & Pessina, A. Neolithic agriculture in Italy: an update of archaeobotanical data with particular emphasis on northern settlements. In The Origins and Spread of Domestic Plants in Southwest Asia and Europe 157–170 (Routledge, 2016).

  • Arobba, D., Panelli, C., Caramiello, R., Gabriele, M. & Maggi, R. Cereal remains, plant impressions and 14C direct dating from the Neolithic pottery of Arene Candide Cave (Finale Ligure, NW Italy). J. Archaeol. Sci. Rep. 12, 395–404 (2017).

    Google Scholar 

  • Ucchesu, M., Sau, S. & Lugliè, C. Crop and wild plant exploitation in Italy during the Neolithic period: New data from Su Mulinu Mannu, Middle Neolithic site of Sardinia. J. Archaeol. Sci. Rep. 14, 1–11 (2017).

    Google Scholar 

  • Scorrano, G. et al. Effect of Neolithic transition on an Italian community: Mora Cavorso (Jenne, Rome). Archaeol. Anthropol. Sci. 11, 1443–1459 (2019).

    Article 

    Google Scholar 

  • De Angelis, F. et al. Exploring mobility in Italian Neolithic and Copper Age communities. Sci. Rep. 11, 1–14 (2021).

    Article 

    Google Scholar 

  • Oxilia, G. et al. Exploring late Paleolithic and Mesolithic diet in the Eastern Alpine region of Italy through multiple proxies. Am. J. Phys. Anthropol. 174, 232–253 (2021).

    Article 

    Google Scholar 

  • Fahmy, A. G. E. Palaeoethnobotanical studies of the Neolithic settlement in Hidden Valley, Farafra Oasis, Egypt. Veg. Hist. Archaeobot. 10, 235–246 (2001).

    Article 

    Google Scholar 

  • Reed, K. From the field to the hearth: plant remains from Neolithic Croatia (ca. 6000–4000 cal bc). Veg. Hist. Archaeobot. 24, 601–619 (2015).

    Article 

    Google Scholar 

  • Lucarini, G., Radini, A., Barton, H. & Barker, G. The exploitation of wild plants in Neolithic North Africa. Use-wear and residue analysis on non-knapped stone tools from the Haua Fteah cave, Cyrenaica, Libya. Quat. Int. 410, 77–92 (2016).

    Article 

    Google Scholar 

  • García-Granero, J. J., Urem-Kotsou, D., Bogaard, A. & Kotsos, S. Cooking plant foods in the northern Aegean: microbotanical evidence from Neolithic Stavroupoli (Thessaloniki, Greece). Quat. Int. 496, 140–151 (2018).

    Article 

    Google Scholar 

  • Bouby, L. et al. Early Neolithic (ca. 5850-4500 cal BC) agricultural diffusion in the Western Mediterranean: an update of archaeobotanical data in SW France. PLoS One 15, e0230731 (2020).

    Article 
    CAS 

    Google Scholar 

  • Delhon, C., Binder, D., Verdin, P. & Mazuy, A. Phytoliths as a seasonality indicator? The example of the Neolithic site of Pendimoun, south-eastern France. Veg. Hist. Archaeobot. 29, 229–240 (2020).

    Article 

    Google Scholar 

  • Lu, H. et al. Phytoliths analysis for the discrimination of foxtail millet (Setaria italica) and common millet (Panicum miliaceum). PLoS One 4, e4448 (2009).

    Article 

    Google Scholar 

  • Celant, A. Indagini paleobotaniche su macroresti vegetali dai siti neo-eneolitici del territorio di Roma. In Roma prima del mito. Abitati e necropoli dal Neolitico alla prima età dei Metalli nel territorio di Roma (VI-III millennio a.C.) (eds Anzidei, A. P. & Carboni, C.) Vol. 2, 687–704 (Archaeopress Archaeol., 2020).

  • Carra, M. et al. Plant foods in the Late Palaeolithic of Southern Italy and Sicily: Integrating carpological and dental calculus evidence. Quat. Int. https://doi.org/10.1016/j.quaint.2022.06.007 (2022) .

  • Bednar, G. E. et al. Starch and fiber fractions in selected food and feed ingredients affect their small intestinal digestibility and fermentability and their large bowel fermentability in vitro in a canine model. J. Nutr. 131, 276–286 (2001).

    Article 
    CAS 

    Google Scholar 

  • Hoover, R., Hughes, T., Chung, H. J. & Liu, Q. Composition, molecular structure, properties, and modification of pulse starches: a review. Food Res. Int. 43, 399–413 (2010).

    Article 
    CAS 

    Google Scholar 

  • Wani, I. A. et al. Isolation, composition, and physicochemical properties of starch from legumes: a review. Starch‐Stärke 68, 834–845 (2016).

    Article 
    CAS 

    Google Scholar 

  • Tayade, R., Kulkarni, K. P., Jo, H., Song, J. T. & Lee, J. D. Insight into the prospects for the improvement of seed starch in legume—a review. Front. Plant Sci. 10, 1213 (2019).

    Article 

    Google Scholar 

  • Stafford, H. A. Distribution of tartaric acid in the leaves of certain angiosperms. Am. J. Bot. 46, 347–352 (1959).

    Article 
    CAS 

    Google Scholar 

  • DeBolt, S., Cook, D. R. & Ford, C. M. L-Tartaric acid synthesis from vitamin C in higher plants. Proc. Natl. Acad. Sci. USA 103, 5608–5613 (2006).

    Article 
    CAS 

    Google Scholar 

  • Fernández-García, E. et al. Carotenoids bioavailability from foods: from plant pigments to efficient biological activities. Food Res. Int. 46, 438–450 (2012).

    Article 

    Google Scholar 

  • Gliszczyńska, A. & Brodelius, P. E. Sesquiterpene coumarins. Phytochem. Rev. 11, 77–96 (2012).

    Article 

    Google Scholar 

  • Eerkens, J. The preservation and identification of Piñon resins by GC‐MS in pottery from the Western Great Basin. Archaeometry 44, 95–105 (2002).

    Article 
    CAS 

    Google Scholar 

  • Barnard, H. et al. Mixed results of seven methods for organic residue analysis applied to one vessel with the residue of a known foodstuff. J. Archaeol. Sci. 34, 28–37 (2007).

    Article 

    Google Scholar 

  • Wysocka, W., Przybył, A. & Brukwicki, T. The structure of angustifoline, an alkaloid of Lupinus angustifolius, in solution. Monatsh. Chem. 125, 1267–1272 (1994).

    Article 
    CAS 

    Google Scholar 

  • Ohmiya, S., Saito, K., & Murakoshi, I. Lupine alkaloids. In The alkaloids: Chemistry and Pharmacology Vol. 47, 1–114) (Academic Press, 1995).

  • Mancinotti, D., Frick, K. M. & Geu-Flores, F. Biosynthesis of quinolizidine alkaloids in lupins: mechanistic considerations and prospects for pathway elucidation. Nat. Prod. Rep. 39, 1423–1437 (2022).

    Article 
    CAS 

    Google Scholar 

  • Silvestri, L., Achino, K. F., Gatta, M., Rolfo, M. F. & Salari, L. Grotta Mora Cavorso: physical, material and symbolic boundaries of life and death practices in a Neolithic cave of central Italy. Quat. Int. 539, 29–38 (2020).

    Article 

    Google Scholar 

  • Steele, V. J., Stern, B. & Stott, A. W. Olive oil or lard?: distinguishing plant oils from animal fats in the archaeological record of the eastern Mediterranean using gas chromatography/combustion/isotope ratio mass spectrometry. Rapid Commun. Mass Spectrom. 24, 3478–3484 (2010).

    Article 
    CAS 

    Google Scholar 

  • Buonasera, T. Investigating the presence of ancient absorbed organic residues in groundstone using GC–MS and other analytical techniques: a residue study of several prehistoric milling tools from central California. J. Archaeol. Sci. 34, 1379–1390 (2007).

    Article 

    Google Scholar 

  • Luong, S. et al. Development and application of a comprehensive analytical workflow for the quantification of non-volatile low molecular weight lipids on archaeological stone tools. Anal. Met. 9, 4349–4362 (2017).

    Article 
    CAS 

    Google Scholar 

  • Baeten, J., Jervis, B., De Vos, D. & Waelkens, M. Molecular evidence for the mixing of Meat, Fish and Vegetables in Anglo‐Saxon coarseware from Hamwic, UK. Archaeometry 55, 1150–1174 (2013).

    Article 
    CAS 

    Google Scholar 

  • Evershed, R. P. Chemical composition of a bog body adipocere. Archaeometry 34, 253–265 (1992).

    Article 
    CAS 

    Google Scholar 

  • Garnier, N., Bernal-Casasola, D., Driard, C. & Pinto, I. V. Looking for ancient fish products through invisible biomolecular residues in the roman production vats from the Atlantic. Coast J. Marit. Archaeol. 13, 285–328 (2018).

    Article 

    Google Scholar 

  • Copley, M. S., Bland, H. A., Rose, P., Horton, M. & Evershed, R. P. Gas chromatographic, mass spectrometric and stable carbon isotopic investigations of organic residues of plant oils and animal fats employed as illuminants in archaeological lamps from Egypt. Analyst 130, 860–871 (2005).

    Article 
    CAS 

    Google Scholar 

  • Reber, E. A. & Hart, J. P. Pine resins and pottery sealing: analysis of absorbed and visible pottery residues from central New York State. Archaeometry 50, 999–1017 (2008).

    Article 
    CAS 

    Google Scholar 

  • Simopoulos, A. P. Omega‐3 fatty acids in wild plants, nuts and seeds. Asia Pac. J. Clin. Nutr. 11, S163–S173 (2002).

    Article 
    CAS 

    Google Scholar 

  • Harris, W. S. et al. Stearidonic acid-enriched soybean oil increased the omega-3 index, an emerging cardiovascular risk marker. Lipids 43, 805–811 (2008).

    Article 
    CAS 

    Google Scholar 

  • Gismondi, A., Rolfo, M. F., Leonardi, D., Rickards, O. & Canini, A. Identification of ancient Olea europaea L. and Cornus mas L. seeds by DNA barcoding. C. R. Biol. 335, 472–479 (2012).

    Article 
    CAS 

    Google Scholar 

  • Steffens, W. & Wirth, M. Freshwater fish-an important source of n-3 polyunsaturated fatty acids: a review. Fish. Aquat. Sci. 13, 5–16 (2005).

    Google Scholar 

  • Swanson, D., Block, R. & Mousa, S. A. Omega-3 fatty acids EPA and DHA: health benefits throughout life. Adv. Nutr. 3, 1–7 (2012).

    Article 
    CAS 

    Google Scholar 

  • Wiermann, R., & Gubatz, S. Pollen wall and sporopollenin. In International Review of Cytology 35–72 (Academic Press, 1992).

  • Cristiani, E., Radini, A., Edinborough, M. & Borić, D. Dental calculus reveals Mesolithic foragers in the Balkans consumed domesticated plant foods. Proc. Natl. Acad. Sci. USA 113, 10298–10303 (2016).

    Article 
    CAS 

    Google Scholar 

  • Hardy, K. et al. Dental calculus reveals potential respiratory irritants and ingestion of essential plant-based nutrients at Lower Palaeolithic Qesem Cave Israel. Quat. Int. 398, 129–135 (2016).

    Article 

    Google Scholar 

  • Radini, A. et al. Neanderthals, trees and dental calculus: new evidence from El Sidrón. Antiquity 90, 290–301 (2016).

    Article 

    Google Scholar 

  • Lippi, M. M., Pisaneschi, L., Sarti, L., Lari, M. & Moggi-Cecchi, J. Insights into the Copper-Bronze Age diet in central Italy: plant microremains in dental calculus from Grotta dello Scoglietto (Southern Tuscany, Italy). J. Archaeol. Sci. Rep. 15, 30–39 (2017).

    Google Scholar 

  • Modi, A. et al. Combined metagenomic and archaeobotanical analyses on human dental calculus: a cross-section of lifestyle conditions in a Copper Age population of central Italy. Quat. Int. https://doi.org/10.1016/j.quaint.2021.12.003 (2021).

  • Warinner, C. et al. Pathogens and host immunity in the ancient human oral cavity. Nat. Genet. https://doi.org/10.1038/ng.2906 (2014).

  • Lieverse, A. R. Diet and the aetiology of dental calculus. Int. J. Osteoarchaeol. 9, 219–232 (1999).

    <a data-track="click" rel="nofollow noopener" data-track-label="10.1002/(SICI)1099-1212(199907/08)9:43.0.CO;2-V” data-track-action=”article reference” href=”https://doi.org/10.1002%2F%28SICI%291099-1212%28199907%2F08%299%3A4%3C219%3A%3AAID-OA475%3E3.0.CO%3B2-V” aria-label=”Article reference 110″ data-doi=”10.1002/(SICI)1099-1212(199907/08)9:43.0.CO;2-V”>Article 

    Google Scholar 

  • Lukacs, J. R. & Largaespada, L. L. Explaining sex differences in dental caries prevalence: saliva, hormones, and “life‐history” etiologies. Am. J. Hum. Biol. 18, 540–555 (2006).

    Article 

    Google Scholar 

  • Moore, P. D., Webb, J. A., & Collison, M. E. Pollen Analysis (Blackwell Scientific Publications, 1991).

  • Borojević, K., Forenbaher, S., Kaiser, T. & Berna, F. Plant use at Grapčeva cave and in the eastern Adriatic Neolithic. J. Field Archaeol. 33, 279–303 (2008).

    Article 

    Google Scholar 

  • Martin, L., Jacomet, S. & Tiebault, S. Plant economy during the Neolithic in a mountain context: the case of “Le Chenet des Pierres” in the French Alps (Bozel-Savoie, France). Veg. Hist. Archaeobot. 17, 113–122 (2008).

    Article 

    Google Scholar 

  • Moser, D., Di Pasquale, G., Scarciglia, F. & Nelle, O. Holocene mountain forest changes in central Mediterranean: soil charcoal data from the Sila Massif (Calabria, southern Italy). Quat. Int. 457, 113–130 (2017).

    Article 

    Google Scholar 

  • D’Agostino, A. et al. Pollen record of the Late Pleistocene–Holocene stratigraphic sequence and current plant biodiversity from Grotta Mora Cavorso (Simbruini Mountains, Central Italy). Ecol. Evol. 12, e9486 (2022).

  • Radaeski, J. N., Bauermann, S. G. & Pereira, A. B. Poaceae pollen from Southern Brazil: distinguishing grasslands (campos) from forests by analyzing a diverse range of Poaceae species. Front. Plant Sci. 7, 1833 (2016).

    Article 

    Google Scholar 

  • Turner, S. D. & Brown, A. G. Vitis pollen dispersal in and from organic vineyards: I. Pollen trap and soil pollen data. Rev. Palaeobot. Palynol. 129, 117–132 (2004).

    Article 

    Google Scholar 

  • Marvelli, S., De’Siena, S., Rizzoli, E. & Marchesini, M. The origin of grapevine cultivation in Italy: the archaeobotanical evidence. Ann. Bot. 3, 155–163 (2013).

    Google Scholar 

  • Riaz, S. et al. Genetic diversity analysis of cultivated and wild grapevine (Vitis vinifera L.) accessions around the Mediterranean basin and Central Asia. BMC Plant Biol. 18, 1–14 (2018).

    Article 

    Google Scholar 

  • Arnold, C., Gillet, F., & Gobat, J. M. Situation de la vigne sauvage Vitis vinifera subsp. silvestris en Europe. Vitis 159–170 (1998).

  • Terral, J. F. et al. Evolution and history of grapevine (Vitis vinifera) under domestication: new morphometric perspectives to understand seed domestication syndrome and reveal origins of ancient European cultivars. Ann. Bot. 105, 443–455 (2010).

    Article 

    Google Scholar 

  • Buckley, S., Usai, D., Jakob, T., Radini, A. & Hardy, K. Dental calculus reveals unique insights into food items, cooking and plant processing in prehistoric central Sudan. PLoS One 9, e100808 (2014).

    Article 

    Google Scholar 

  • Petrov, P. R., Popova, E. D. & Zlatanova, D. P. Niche partitioning among the red fox Vulpes vulpes (L.), stone marten Martes foina (Erxleben) and pine marten Martes martes (L.) in two mountains in Bulgaria. Acta Zool. Bulg. 68, 375–390 (2016).

    Google Scholar 

  • Mikrjukov, K. A. Revision of genera and species composition of lower Centroheliozoa. II. Family Raphidiophryidae n. tam. Arch. Protistenkd. 147, 205–212 (1996).

    Article 

    Google Scholar 

  • Cavalier-Smith, T. & von der Heyden, S. Molecular phylogeny, scale evolution and taxonomy of centrohelid heliozoa. Mol. Phylogen. Evol. 44, 1186–1203 (2007).

    Article 
    CAS 

    Google Scholar 

  • Mertens, K. N., Rengefors, K., Moestrup, Ø. & Ellegaard, M. A review of recent freshwater dinoflagellate cysts: taxonomy, phylogeny, ecology and palaeocology. Phycologia 51, 612–619 (2012).

    Article 

    Google Scholar 

  • Zlatogursky, V. V. Raphidiophrys heterophryoidea sp. nov. (Centrohelida: Raphidiophryidae), the first heliozoan species with a combination of siliceous and organic skeletal elements. Eur. J. Protist. 48, 9–16 (2012).

    Article 

    Google Scholar 

  • Prokina, K. I. & Mylnikov, A. P. Centrohelid heliozoans from freshwater habitats of different types of South Patagonia and Tierra del Fuego, Chile. Inland Water Biol. 12, 10–20 (2019).

    Article 

    Google Scholar 

  • Siemensma, F. J. & Roijackers, M. M. A study of new and little- known acanthocystid heliozoans, and a proposed division of the genus Acanthocystis (Actinopoda, Heliozoea). Arch. Protistenkd. 135, 197 (1988a).

    Article 

    Google Scholar 

  • Siemensma, F. J. & Roijackers, M. M. The genus Raphidiophrys (Actinopoda, Heliozoea): scale morphology and species distinctions. Arch. Protistenkd. 136 237–248 (1988).

  • Taylor, W.D. & Sanders, R. W. PROTOZOA. In Ecology and Classification of North American Freshwater Invertebrates (eds Thorp, J. H. & Covich, A. P.) 43–96 (Academic Press, 2001).

  • Manconi, R., & Pronzato, R. Global diversity of sponges (Porifera: Spongillina) in freshwater. In Freshwater Animal Diversity Assessment 27–33 (Springer, Dordrecht, 2007).

  • Malone, C. & Stoddart, S. The neolithic site of San Marco, Gubbio (Perugia), Umbria: survey and excavation 1985–7. Pap. Br. Sch. Rome 60, 1–69 (1992).

    Article 

    Google Scholar 

  • Rottoli, M. La Marmotta, Anguillara Sabazia (RM). Scavi 1989. Analisi paletnobotaniche: prime risultanze, Appendice 1 M.A. In La Marmotta” (Anguillara Sabazia, RM). Scavi 1989. Un abitato perilacustre di età neolitica (eds. Fugazzola Delpino, M. A., D’Eugenio, G. & Pessina, A.) Bullettino di Paletnologia Italiana 84, 305–315 (1993).

  • Pini, R. Late Neolithic vegetation history at the pile‐dwelling site of Palù di Livenza (northeastern Italy). J. Quat. Sci. 19, 769–781 (2004).

    Article 

    Google Scholar 

  • Tinner, W. et al. Holocene environmental and climatic changes at Gorgo Basso, a coastal lake in southern Sicily, Italy. Quat. Sci. Rev. 28, 1498–1510 (2009).

    Article 

    Google Scholar 

  • Bieniek, A. Archaeobotanical analysis of some early Neolithic settlements in the Kujawy region, central Poland, with potential plant gathering activities emphasized. Veg. Hist. Archaeobot. 11, 33–40 (2002).

    Article 

    Google Scholar 

  • Tolar, T., Jacomet, S., Velušček, A. & Čufar, K. Plant economy at a late Neolithic lake dwelling site in Slovenia at the time of the Alpine Iceman. Veg. Hist. Archaeobot. 20, 207–222 (2011).

    Article 

    Google Scholar 

  • D’Agostino, A. et al. Investigating plant micro-remains embedded in dental calculus of the Phoenician inhabitants of Motya (Sicily, Italy). Plants 9, 1395 (2020).

    Article 

    Google Scholar 

  • Mercader, J. et al. Exaggerated expectations in ancient starch research and the need for new taphonomic and authenticity criteria. Facets 3, 777–798 (2018).

    Article 

    Google Scholar 

  • Adojoh, O., Fabienne, M., Duller, R. & Osterloff, P. Taxonomy and phytoecology of palynomorphs and non-pollen palynomorphs: a refined compendium from the West Africa Margin. Biodivers. Int. J. 3, 188–200 (2019).

    Article 

    Google Scholar 

  • Knapp, M., Clarke, A. C., Horsburgh, K. A. & Matisoo-Smith, E. A. Setting the stage building and working in an ancient DNA laboratory. Ann. Anat. 194, 3 (2012).

    Article 
    CAS 

    Google Scholar 

  • Knapp, M., Lalueza-Fox, C. & Hofreiter, M. Re-inventing ancient human DNA. Investig. Genet. 6, 1 (2015).

    Article 

    Google Scholar 

  • Gismondi, A. et al. Grapevine carpological remains revealed the existence of a Neolithic domesticated Vitis vinifera L. specimen containing ancient DNA partially preserved in modern ecotypes. J. Archaeol. Sci. 69, 75–84 (2016).

    Article 
    CAS 

    Google Scholar 

  • Llamas, B. et al. From the field to the laboratory: controlling DNA contamination in human ancient DNA research in the high-throughput sequencing era. Sci. Technol. Archaeol. Res. 3, 1–14 (2017).

  • Le Moyne, C. & Crowther, A. Effects of chemical pre-treatments on modified starch granules: recommendations for dental calculus decalcification for ancient starch research. J. Archaeol. Sci. Rep. 35, 102762 (2021).

    Google Scholar 

  • Rolfo, M. F., Achino, K. F., Fusco, I., Salari, L. & Silvestri, L. Reassessing human occupation patterns in the inner central Apennines in prehistory: the case-study of Grotta Mora Cavorso. J. Archaeol. Sci. Rep. 7, 358–367 (2016).

    Google Scholar 


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