Gruber N, Galloway JN. An Earth-system perspective of the global nitrogen cycle. Nature. 2008;451:293–6.
Sohm JA, Webb EA, Capone DG. Emerging patterns of marine nitrogen fixation. Nat Rev Microbiol. 2011;9:499–508.
Karl D, Letelier R, Tupas L, Dore J, Christian J, Hebel D. The role of nitrogen fixation in biogeochemical cycling in the subtropical North Pacific Ocean. Nature. 1997;388:533–8.
Ko YH, Lee K, Takahashi T, Karl DM, Kang S-H, Lee E. Carbon-based estimate of nitrogen fixation-derived net community production in N-depleted ocean gyres. Glob Biogeochem Cycles. 2018;32:1241–52.
Deutsch C, Sigman DM, Thunell RC, Meckler AN, Haug GH. Isotopic constraints on glacial/interglacial changes in the oceanic nitrogen budget. Glob Biogeochem Cycles. 2004;18:GB4012.
Falkowski PG. Evolution of the nitrogen cycle and its influence on the biological sequestration of CO2 in the ocean. Nature. 1997;387:272–5.
Michaels AF, Karl DM, Capone DG. Element stoichiometry, new production and nitrogen fixation. Oceanography. 2001;14:68–77.
Moisander PH, Beinart RA, Hewson I, White AE, Johnson KS, Carlson CA, et al. Unicellular cyanobacterial distributions broaden the oceanic N2 fixation domain. Science. 2010;327:1512–4.
Sañudo-Wilhelmy SA, Kustka AB, Gobler CJ, Hutchins DA, Yang M, Lwiza K, et al. Phosphorus limitation of nitrogen fixation by Trichodesmium in the central Atlantic Ocean. Nature. 2001;411:66–9.
Knapp AN, Casciotti KL, Berelson WM, Prokopenko MG, Capone DG. Low rates of nitrogen fixation in eastern tropical South Pacific surface waters. Proc Natl Acad Sci USA. 2016;113:4398–403.
Ward BA, Dutkiewicz S, Moore CM, Follows MJ. Iron, phosphorus, and nitrogen supply ratios define the biogeography of nitrogen fixation. Limnol Oceanogr. 2013;58:2059–75.
Wang W-L, Moore JK, Martiny AC, Primeau FW. Convergent estimates of marine nitrogen fixation. Nature. 2019;566:205–11.
Weber T, Deutsch C. Local versus basin-scale limitation of marine nitrogen fixation. Proc Natl Acad Sci USA. 2014;111:8741–6.
Mills MM, Ridame C, Davey M, La Roche J, Geider RJ. Iron and phosphorus co-limit nitrogen fixation in the eastern tropical North Atlantic. Nature. 2004;429:292–4.
Luo YW, Lima ID, Karl DM, Deutsch CA, Doney SC. Data-based assessment of environmental controls on global marine nitrogen fixation. Biogeosciences. 2014;11:691–708.
Moore CM, Mills MM, Achterberg EP, Geider RJ, LaRoche J, Lucas MI, et al. Large-scale distribution of Atlantic nitrogen fixation controlled by iron availability. Nat Geosci. 2009;2:867–71.
Ratten J-M, LaRoche J, Desai DK, Shelley RU, Landing WM, Boyle E, et al. Sources of iron and phosphate affect the distribution of diazotrophs in the North Atlantic. Deep Sea Res Part II: Topical Stud Oceanogr. 2015;116:332–41.
Straub M, Sigman DM, Ren H, Martínez-García A, Meckler AN, Hain MP, et al. Changes in North Atlantic nitrogen fixation controlled by ocean circulation. Nature. 2013;501:200–4.
Dugdale R, Menzel DW, Ryther JH. Nitrogen fixation in the Sargasso Sea. Deep Sea Res. 1961;7:297–300.
Capone DG, Burns JA, Montoya JP, Subramaniam A, Mahaffey C, Gunderson T, et al. Nitrogen fixation by Trichodesmium spp.: an important source of new nitrogen to the tropical and subtropical North Atlantic Ocean. Glob Biogeochem Cycles. 2005;19:GB2024.
Martínez-Pérez C, Mohr W, Löscher CR, Dekaezemacker J, Littmann S, Yilmaz P, et al. The small unicellular diazotrophic symbiont, UCYN-A, is a key player in the marine nitrogen cycle. Nat Microbiol. 2016;1:16163.
Delmont TO, Quince C, Shaiber A, Esen OC, Lee ST, Rappe MS, et al. Nitrogen-fixing populations of Planctomycetes and Proteobacteria are abundant in surface ocean metagenomes. Nat Microbiol. 2018;3:804–13.
Tang W, Wang S, Fonseca-Batista D, Dehairs F, Gifford S, Gonzalez AG, et al. Revisiting the distribution of oceanic N2 fixation and estimating diazotrophic contribution to marine production. Nat Commun. 2019;10:831.
Cassar N, Tang W, Gabathuler H, Huang K. Method for high frequency underway N2 fixation measurements: flow-through incubation acetylene reduction assays by cavity ring down laser absorption spectroscopy (FARACAS). Anal Chem. 2018;90:2839–51.
Bruland KW, Rue EL, Smith GJ, DiTullio GR. Iron, macronutrients and diatom blooms in the Peru upwelling regime: brown and blue waters of Peru. Mar Chem. 2005;93:81–103.
Raimbault P, Slawyk G, Coste B, Fry J. Feasibility of using an automated colorimetric procedure for the determination of seawater nitrate in the 0 to 100 nM range: examples from field and culture. Marine Biol. 1990;104:347–51.
Strickland JD, Parsons T. A practical handbook of seawater analysis. Ottawa; 1972.
Cutter G, Andersson P, Codispoti L, Croot P, Francois R, Lohan M, et al. Sampling and sample-handling protocols for GEOTRACES cruises. GEOTRACES; 2010.
Lagerström M, Field M, Séguret M, Fischer L, Hann S, Sherrell R. Automated on-line flow-injection ICP-MS determination of trace metals (Mn, Fe, Co, Ni, Cu and Zn) in open ocean seawater: application to the GEOTRACES program. Mar Chem. 2013;155:71–80.
Berthelot H, Benavides M, Moisander PH, Grosso O, Bonnet S. High-nitrogen fixation rates in the particulate and dissolved pools in the Western Tropical Pacific (Solomon and Bismarck Seas). Geophys Res Lett. 2017;44:8414–23.
Montoya JP, Voss M, Kahler P, Capone DG. A simple, high-precision, high-sensitivity tracer assay for N2 fixation. Appl Environ Microbiol. 1996;62:986–93.
Cassar N, Barnett BA, Bender ML, Kaiser J, Hamme RC, Tilbrook B. Continuous high-frequency dissolved O2/Ar measurements by equilibrator inlet mass spectrometry. Anal Chem. 2009;81:1855–64.
Craig H, Hayward T. Oxygen supersaturation in the ocean: biological versus physical contributions. Science. 1987;235:199–202.
Zehr J, Turner P. Nitrogen fixation: nitrogenase genes and gene expression. Method Microbiol. 2001;30:271–86.
Turk KA, Rees AP, Zehr JP, Pereira N, Swift P, Shelley R, et al. Nitrogen fixation and nitrogenase (nifH) expression in tropical waters of the eastern North Atlantic. ISME J 2011;5:1201–12.
Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, et al. QIIME allows analysis of high-throughput community sequencing data. Nat Methods. 2010;7:335–6.
Edgar RC. Search and clustering orders of magnitude faster than BLAST. Bioinformatics. 2010;26:2460–1.
Edgar RC. UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nature Methods. 2013;10:996.
Church MJ, Jenkins BD, Karl DM, Zehr JP. Vertical distributions of nitrogen-fixing phylotypes at Stn ALOHA in the oligotrophic North Pacific Ocean. Aquat Microbial Ecol. 2005;38:3–14.
Robidart JC, Church MJ, Ryan JP, Ascani F, Wilson ST, Bombar D, et al. Ecogenomic sensor reveals controls on N2-fixing microorganisms in the North Pacific Ocean. The. ISME J. 2014;8:1175–85.
Sipler RE, Gong D, Baer SE, Sanderson MP, Roberts QN, Mulholland MR, et al. Preliminary estimates of the contribution of Arctic nitrogen fixation to the global nitrogen budget. Limnol Oceanogr Lett. 2017;2:159–66.
Shiozaki T, Fujiwara A, Ijichi M, Harada N, Nishino S, Nishi S, et al. Diazotroph community structure and the role of nitrogen fixation in the nitrogen cycle in the Chukchi Sea (western Arctic Ocean). Limnol Oceanogr. 2018;63:2191–205.
Needoba JA, Foster RA, Sakamoto C, Zehr JP, Johnson KS. Nitrogen fixation by unicellular diazotrophic cyanobacteria in the temperate oligotrophic North Pacific Ocean. Limnol Oceanogr. 2007;52:1317–27.
Mulholland MR, Bernhardt PW, Widner BN, Selden CR, Chappell PD, Clayton S, et al. High rates of N2 fixation in temperate, Western North Atlantic coastal waters expand the realm of marine diazotrophy. Glob Biogeochem Cycles. 2019;32:826–40.
Benavides M, Voss M. Five decades of N2 fixation research in the North Atlantic Ocean. Front Mar Sci. 2015;2:40.
Townsend DW. Sources and cycling of nitrogen in the Gulf of Maine. J Mar Syst. 1998;16:283–95.
Fennel K, Wilkin J, Levin J, Moisan J, O’Reilly J, Haidvogel D. Nitrogen cycling in the Middle Atlantic Bight: results from a three-dimensional model and implications for the North Atlantic nitrogen budget. Glob Biogeochem Cycles. 2006;20:GB3007.
Fuglister FC, Voorhis AD. A new method of tracking the Gulf Stream 1. Limnol Oceanogr. 1965;10:R115–24.
Luo YW, Doney SC, Anderson LA, Benavides M, Berman-Frank I, Bode A, et al. Database of diazotrophs in global ocean: abundance, biomass and nitrogen fixation rates. Earth System Science. Data. 2012;4:47–73.
Bonnet S, Caffin M, Berthelot H, Moutin T. Hot spot of N2 fixation in the western tropical South Pacific pleads for a spatial decoupling between N2 fixation and denitrification. Proc Natl Acad Sci USA. 2017;114:2800–1.
Ahmed A, Gauns M, Kurian S, Bardhan P, Pratihary A, Naik H, et al. Nitrogen fixation rates in the eastern Arabian Sea. Estuar Coas Shelf Sci. 2017;191:74–83.
Gandhi N, Singh A, Prakash S, Ramesh R, Raman M, Sheshshayee M, et al. First direct measurements of N2 fixation during a Trichodesmium bloom in the eastern Arabian Sea. Glob Biogeochem Cycles. 2011;25:GB4014.
Xu Y, Chant R, Gong D, Castelao R, Glenn S, Schofield O. Seasonal variability of chlorophyll a in the Mid-Atlantic Bight. Cont Shelf Res. 2011;31:1640–50.
Moline MA, Frazer TK, Chant R, Glenn S, Jacoby CA, Reinfelder JR, et al. Biological responses in a dynamic buoyant river plume. Oceanography. 2008;21:70–89.
Zehr JP, Mellon MT, Zani S. New nitrogen-fixing microorganisms detected in oligotrophic oceans by amplification of nitrogenase (nifH) genes. Appl Environ Microbiol. 1998;64:3444–50.
Langlois RJ, Hümmer D, LaRoche J. Abundances and distributions of the dominant nifH phylotypes in the Northern Atlantic Ocean. Appl Environ Microbiol. 2008;74:1922–31.
Benavides M, Moisander PH, Daley MC, Bode A, Arístegui J. Longitudinal variability of diazotroph abundances in the subtropical North Atlantic Ocean. J Plankton Res. 2016;38:662–72.
Stenegren M, Caputo A, Berg C, Bonnet S, Foster RA. Distribution and drivers of symbiotic and free-living diazotrophic cyanobacteria in the western tropical South Pacific. Biogeosciences. 2018;15:1559–78.
Chen M, Lu Y, Jiao N, Tian J, Kao S-J, Zhang Y. Biogeographic drivers of diazotrophs in the western Pacific Ocean. Limnol Oceanogr. 2019;64:1403–21.
He R, Chen K, Fennel K, Gawarkiewicz GG, McGillicuddy DJ Jr. Seasonal and interannual variability of physical and biological dynamics at the shelfbreak front of the Middle Atlantic Bight: nutrient supply mechanisms. Biogeosciences. 2011;8:2935–46.
Nixon SW, Ammerman JW, Atkinson LP, Berounsky VM, Billen G, Boicourt WC, et al. The fate of nitrogen and phosphorus at the land-sea margin of the North Atlantic Ocean. Biogeochemistry. 1996;35:141–80.
Wu J, Sunda W, Boyle EA, Karl DM. Phosphate depletion in the western North Atlantic Ocean. Science. 2000;289:759–62.
Coles V, Hood R. Modeling the impact of iron and phosphorus limitations on nitrogen fixation in the Atlantic Ocean. Biogeosciences. 2007;4:455–79.
Palter JB, Lozier MS, Sarmiento JL, Williams RG. The supply of excess phosphate across the Gulf Stream and the maintenance of subtropical nitrogen fixation. Glob Biogeochem Cycles. 2011;25:GB4007.
Rijkenberg MJ, Middag R, Laan P, Gerringa LJ, van Aken HM, Schoemann V, et al. The distribution of dissolved iron in the West Atlantic Ocean. PLoS ONE. 2014;9:e101323.
Boatman TG, Oxborough K, Gledhill M, Lawson T, Geider RJ. An integrated response of Trichodesmium erythraeum IMS101 growth and photo-physiology to iron, CO2, and light intensity. Front Microbiol. 2018;9:624.
Parekh P, Follows MJ, Boyle EA. Decoupling of iron and phosphate in the global ocean. Glob Biogeochem Cycles. 2005;19:GB2020.
Sedwick P, Bernhardt P, Mulholland M, Najjar R, Blumen L, Sohst B, et al. Assessing phytoplankton nutritional status and potential impact of wet deposition in seasonally oligotrophic waters of the Mid-Atlantic Bight. Geophys Res Lett. 2018;45:3203–11.
Kustka A, Sañudo‐Wilhelmy S, Carpenter EJ, Capone DG, Raven JA. A revised estimate of the iron use efficiency of nitrogen fixation, with special reference to the marine cyanobacterium Trichodesmium spp.(Cyanophyta)1. J Phycol. 2003;39:12–25.
Buck KN, Sohst B, Sedwick PN. The organic complexation of dissolved iron along the U.S. GEOTRACES (GA03) North Atlantic Section. Deep Sea Res Part II: Top Stud Oceanogr. 2015;116:152–65.
Lopez JS, Lee L, Mackey KRM. The toxicity of copper to Crocosphaera watsonii and other marine phytoplankton: a systematic review. Front Mar Sci. 2019;5:511.
Boyle EA, Huested SS, Jones SP. On the distribution of copper, nickel, and cadmium in the surface waters of the North Atlantic and North Pacific Ocean. J Geophys Res. 1981;86(C9):8048–66.
Roshan S, Wu J. The distribution of dissolved copper in the tropical-subtropical north Atlantic across the GEOTRACES GA03 transect. Mar Chem. 2015;176:189–98.
Semeniuk DM, Taylor RL, Bundy RM, Johnson WK, Cullen JT, Robert M, et al. Iron–copper interactions in iron-limited phytoplankton in the northeast subarctic Pacific Ocean. Limnol Oceanogr. 2016;61:279–97.
Moffett JW, Tuit CB, Ward BB. Chelator-induced inhibition of copper metalloenzymes in denitrifying bacteria. Limnol Oceanogr. 2012;57:272–80.
Tucker DL, Hirsh K, Li H, Boardman B, Sherman LA. The manganese stabilizing protein (MSP) and the control of O2 evolution in the unicellular, diazotrophic cyanobacterium, Cyanothece sp. ATCC 51142. Biochim Biophys Acta. 2001;1504:409–22.
Hood G, Ramachandran V, East AK, Downie JA, Poole PS. Manganese transport is essential for N2-fixation by Rhizobium leguminosarum in bacteroids from galegoid but not phaseoloid nodules. Environ Microbiol. 2017;19:2715–26.
Hulten Mv, Middag R, Dutay J-C, Baar Hd, Roy-Barman M, Gehlen M, et al. Manganese in the west Atlantic Ocean in the context of the first global ocean circulation model of manganese. Biogeosciences. 2017;14:1123–52.
Paytan A, McLaughlin K. The oceanic phosphorus cycle. Chem Rev. 2007;107:563–76.
Fonseca-Batista D, Li X, Riou V, Michotey V, Deman F, Fripiat F, et al. Evidence of high N2 fixation rates in the temperate northeast Atlantic. Biogeosciences. 2019;16:999–1017.
Breitbarth E, Oschlies A, LaRoche J. Physiological constraints on the global distribution of Trichodesmium – effects of temperature on diazotrophy. Biogeosciences. 2007;4:53–61.
Sohm JA, Capone DG. Phosphorus dynamics of the tropical and subtropical north Atlantic: Trichodesmium spp. versus bulk plankton. Mar Ecol Prog Ser. 2006;317:21–8.
Lipschultz F, Owens NJ. An assessment of nitrogen fixation as a source of nitrogen to the North Atlantic Ocean. Biogeochemistry. 1996;35:261–74.
Rivero-Calle S, Del Castillo CE, Gnanadesikan A, Dezfuli A, Zaitchik B, Johns DG. Interdecadal Trichodesmium variability in cold North Atlantic waters. Glob Biogeochem Cycles. 2016;30:1620–38.
Rees AP, Tait K, Widdicombe CE, Quartly GD, McEvoy AJ, Al-Moosawi L. Metabolically active, non-nitrogen fixing, Trichodesmium in UK coastal waters during winter. J Plankton Res. 2016;38:673–8.
Pelegrí J, Csanady G. Nutrient transport and mixing in the Gulf Stream. J Geophys Res. 1991;96(C2):2577–83.
Shiozaki T, Takeda S, Itoh S, Kodama T, Liu X, Hashihama F, et al. Why is Trichodesmium abundant in the Kuroshio? Biogeosciences. 2015;12:6931–43.
Needham DM, Fichot EB, Wang E, Berdjeb L, Cram JA, Fichot CG, et al. Dynamics and interactions of highly resolved marine plankton via automated high-frequency sampling. ISME J. 2018;12:2417–32.
Gérikas Ribeiro C, Lopes dos Santos A, Marie D, Pereira Brandini F, Vaulot D. Small eukaryotic phytoplankton communities in tropical waters off Brazil are dominated by symbioses between Haptophyta and nitrogen-fixing cyanobacteria. ISME J. 2018;12:1360–74.
Harding K, Turk-Kubo KA, Sipler RE, Mills MM, Bronk DA, Zehr JP. Symbiotic unicellular cyanobacteria fix nitrogen in the Arctic Ocean. Proc Natl Acad Sci USA. 2018;115:13371–5.
Collos Y, Vaquer A, Souchu P. Acclimation of nitrate uptake by phytoplankton to high substrate levels1. J Phycol. 2005;41:466–78.
Harrison WG, Harris LR, Irwin BD. The kinetics of nitrogen utilization in the oceanic mixed layer: Nitrate and ammonium interactions at nanomolar concentrations. Limnol Oceanogr. 1996;41:16–32.
Bombar D, Paerl RW, Riemann L. Marine non-cyanobacterial diazotrophs: moving beyond molecular detection. Trends in Microbiol. 2016;24:916–27.
Knapp AN. The sensitivity of marine N2 fixation to dissolved inorganic nitrogen. Front Microbiol. 2012;3.
Großkopf T, LaRoche J. Direct and indirect costs of dinitrogen fixation in Crocosphaera watsonii WH8501 and possible implications for the nitrogen cycle. Front Microbiol. 2012;3:236.
Krupke A, Mohr W, LaRoche J, Fuchs BM, Amann RI, Kuypers MM. The effect of nutrients on carbon and nitrogen fixation by the UCYN-A–haptophyte symbiosis. ISME J. 2015;9:1635–47.
Martiny AC, Coleman ML, Chisholm SW. Phosphate acquisition genes in Prochlorococcus ecotypes: Evidence for genome-wide adaptation. Proc Natl Acad Sci USA. 2006;103:12552–7.
Rouco M, Frischkorn KR, Haley ST, Alexander H, Dyhrman ST. Transcriptional patterns identify resource controls on the diazotroph Trichodesmium in the Atlantic and Pacific oceans. ISME J. 2018;12:1486–95.
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