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Study areaWe conducted our study in two suburbs in Wellington, New Zealand (Fig. 1). The 4.7-hectare site in the suburb of Kelburn (-41.285°S, 174.770°E) was situated on the grounds of student accommodation for Victoria University of Wellington. The site comprised bungalow houses, two accommodation halls, and access roads and paths. About half of the vegetation at the Kelburn site was a mix of tended grass lawns and gardens containing a variety of native New Zealand plant species, e.g., flax (Phormium spp.), longwood tussock (Carex comans), and cabbage tree (Cordyline australis). The other half was a mix of dense ground cover dominated by invasive weed species and native and exotic trees and shrubs, e.g., pōhutukawa (Metrosideros excelsa), common oak (Quercus robur), kawakawa (Piper excelsum), and taupata (Coprosma repens). The second suburb was Roseneath (−41.292°S, 174.801°E) on a small peninsula on the north-eastern side of Mount Victoria. The site was 8.5 hectares comprising 76 residential properties, public thoroughfares, and footpaths. We conducted fieldwork in the gardens of 25 of these properties. The vegetation varied considerably between gardens, comprising native and introduced garden plants and invasive weeds, especially blackberry (Rubus fruticosus).Figure 1(A) The study was conducted in the suburbs of Kelburn (left yellow dot) and Roseneath (right yellow dot) in the city of Wellington, New Zealand. The black polygon represents the 1475 ha area that will be targeted for ship rat (Rattus rattus) eradication in Wellington city, New Zealand. In each suburb, we radio-collared ship rats and deployed three types of devices (bait stations, chew cards, and WaxTags) to estimate home range and detection parameters. (B) In Kelburn, we radio-collared 14 rats and deployed eight devices. (C) In Roseneath, we radio-collared 16 rats and deployed 30 devices. The yellow circles indicate home range centers of individual rats, the red triangles indicate the location of bait stations and detection devices, and the small black dots indicate the telemetry locations of rats.Full size imageRat capture, radio-collaring, and field methodologyWe set 100 live-capture cage traps (custom-made, spring-loaded traps) in Kelburn from 12 July to 15 August 2020, and another 100 in Roseneath from 20 August to 20 October 2020. We baited cage traps with apple coated in chocolate spread and checked them at least once every 24 h. We set cage traps in areas with complex vegetative groundcover and understorey to maximize capture rates of ship rats (see35), and to provide shelter from inclement weather. We provided additional shelter by inserting bedding inside a tin can placed in the cage traps, along with a plastic cover over the traps to limit exposure to wind and rain. Cage traps were active for 5 days per week on average. We released all non-target species (house mice Mus musculus, European hedgehogs Erinaceus europaeus, and Eurasian blackbirds Turdus merula).We transferred any trap containing a captured rat into a sealed plastic container. Depending on the estimated size of the captured rat, we placed between one and three cotton balls soaked in isoflurane (99.9%, Attane, Piramal Critical Care Inc., Bethlehem, Pennsylvania, USA) inside the plastic container. A rat was anesthetized when it lost balance and was unable to regain balance when we gently rotated the container. We then removed the rat from the cage trap and placed it next to a heat pad with its head close to the cotton balls soaked in isoflurane to maintain anaesthesia while handling them. We fitted all rats weighing  > 110 g with a V1C 118B VHF radio-collar (Lotek, Havelock North, New Zealand). We marked each collared rat with a unique pelage code using a permanent blonde hair dye60. We also recorded biometrics, including sex, weight, and length. When processing was finished, we placed the rat into another container to recover. This container had a heating pad for warmth and an apple for food to avoid a drop in body temperature and hypoglycemia, which are common problems with anaesthesia62. When the rat appeared mobile, energetic, and behaving normally, we released it at the point of capture.We monitored radio-collared rats using a Yagi antenna (Lotek, Havelock North, New Zealand) and a Telonics R-1000 receiver (Telonics Inc., Mesa, Arizona, USA). We conducted radio-telemetry work during August–November 2020, with fixes taken during the day and night. We recorded a total of three fixes per rat per night, taken at two-hour intervals between the hours of sunset (2200 h) and sunrise (0500 h). We mostly attempted one day-time fix (1200 h); however, if a tracked rat was active (determined by a VHF signal that was moving or changing amplitude), we attempted a second fix in the afternoon. To minimize location error, we used the close approach radio-tracking method described by63. Once a successful fix was made, we used a handheld GPS unit to record the location, date, and time. Telemetry fixes were collected for each radio-collared rat for 18–97 days.After approximately one week of radiotracking an animal, we obtained an initial crude estimate of the center of each rat’s home range as the mean of all eastings and northings (based on a minimum of 15 telemetry points per rat). A bait station baited with non-toxic pellets (Protecta Sidekick bait stations, Bell Laboratories Inc., Windsor, Wisconsin, USA), a WaxTag with a peanut butter odor incorporated into the wax (PCR WaxTags, Traps.co.nz, Rolleston, New Zealand), and a chew card (a corflute card baited with peanut butter) were deployed at varying distances (max. 50 m) and cardinal directions from the estimated home range center of each individual rat. This layout maximized the likelihood of encounters with devices, compared with a regular grid-type deployment where some of the devices could fall outside a collared rat’s home range and thus never be encountered. Note that the crude estimate of the location of the home range center for each rat was only used to guide device placement, i.e., it was not used in any statistical analyses, or to describe rat home range sizes. Further, to avoid a choice-type experiment (i.e., all three devices set immediately next to each other), we randomly assigned a distance and cardinal direction to each device type within each rat’s home range but ensured all devices were deployed  > 15 m apart. The three device types were chosen because they are used by Predator Free Wellington to conduct their eradication operations.Every deployed device had a trail camera (Browning Strike Force HD Pro Micro Series, Morgan, Utah, USA) taking video of rats encountering and interacting with the device. We set cameras to take 20 s of video footage when triggered, followed by a 1 s re-trigger interval. We fixed trail cameras to trees at a height of 50 cm above ground level and placed the devices 1.5 m in front of the camera (after64). This strategy allowed accurate identification of pelage codes on marked rats. We cleared vegetation in front of and immediately behind the trail cameras to avoid accidental triggers. We used pegs to mark a 30-cm-radius circle around each device and considered a rat–device encounter when a rat entered that circle. We serviced trail camera–device pairs at least once every three days. This included adding more non-lethal bait to bait stations and peanut butter to monitoring devices, installing new WaxTags or chew cards if they had been destroyed, and replacing batteries and SD cards in trail cameras. We set up 54 trail camera–device pairs. However, due to trail camera malfunctions, we were able to retrieve footage from only 38 cameras, 8 in Kelburn and 30 in Roseneath. Trail camera–device pairs were active for 20–70 days, but we retained data from only the first 20 days for the analyses.Video processingAll video footage was viewed and interpreted by the same individual (HRM) for consistency. We extracted the following information: date and time of rat sightings, rat ID (according to the pelage code, or designated as ‘R’ for unmarked rats), the duration of the visit to a device, whether or not an encounter occurred (as defined above), and whether or not an interaction occurred. We defined an interaction as a rat either gnawing on a chew card or WaxTag or entering a bait station.Data analysisWe combined all ship rat telemetry data with the device encounter and interaction data, and developed a hierarchical Bayesian model to infer factors influencing the key parameters σ, ε0, and θ. The analytical approach builds on that described in65. For the purpose of estimating ε0 and θ, multiple encounters or interactions by the same individual with the same device on the same night were counted as a single encounter or interaction.The VHF telemetry data Zij were composed of xij (eastings) and yij (northings) locations for each individual rat i at site j (either Kelburn or Roseneath). To simplify the notation, we drop the j subscript from all subsequent equations. We modelled the probability of observing Zi as a symmetric bivariate normal variable$$P({Z}_{i})= prod_{i=1}^{{L}_{i}}Normal(Delta {x}_{i}|0,{sigma }_{i}^{2})Normal(Delta {y}_{i}|0,{sigma }_{i}^{2})$$
(1)
where σi is the standard deviation of a normal distribution with zero mean, Li is the number of location fixes for individual i, and Δxi and Δyi are the straight-line distances from the home range center of individual i to xi and yi, respectively.Home range centers can be estimated using various methods, all of which have underlying assumptions (e.g.,66,67). We calculated the home range center for each individual as the mean of all xi and yi, i.e., the centroid of all locations that we recorded for each individual ( > 30 VHF fixes in all instances). Under this formulation, the home range center is assumed to be perfectly observed, an assumption that is supported by the sample size of telemetry locations that we obtained for each individual (see Supplementary Table 266).We modelled σi as a log-normal variable with mean ln(μi), which was a function of the sex of the individual:$$lnleft({sigma }_{i}right)sim Normal(mathit{ln}left({mu }_{i}right), V)$$
(2)
$$lnleft({mu }_{i}right)= {beta }_{0}+ {beta }_{1}{sex}_{i}$$
(3)
where V is the variance of ln(σi), and ln(μi) is a linear function of a categorical variable indicating whether rat i is a male (0) or a female (1). The priors on the β coefficients and V were Normal(0, 10) and InverseGamma(0.01, 0.01), respectively.The encounter data (Eimt) across all devices m and nights t was modelled as a Bernoulli process:$${E}_{imt}sim Bernoulli({gamma }_{imt})$$
(4)
$$logitleft({gamma }_{imt}right)sim MultivariateNormal(logitleft({P}_{imt}right), varSigma )$$
(5)
where γimt is a latent variable representing the degree to which the nightly probability of rat i encountering a given device is not independent of the encounter outcomes of nearby devices, i.e., we assumed there is spatial autocorrelation in the nightly probability of encountering a device. To account for the spatial autocorrelation not explained by the covariates explicitly modelled (i.e., σ and device type, see below), we included an exponential spatial covariance error structure (Σ) as follows:$$varSigma = {nu }^{2}{e}^{-varphi r}$$
(6)
where ν2 is the variance, φ is a correlation distance parameter, and r is the distance (in m) between pairs of devices68,69. Further, because not all devices were available on all nights, Σ was calculated iteratively for each night considering only those devices that were available. We used moderately informative log-normal priors for the covariance parameters to obtain proper posteriors69: ν2 ~ logN(3,1) and φ ~ logN(1,1).The nightly probability of encounter of device m by individual i on night t (Pimt) was calculated using a half-normal detection function70:$${P}_{imt}= {{left({varepsilon }_{0, im}{e}^{left(-frac{{d}_{im}^{2}}{2{sigma }_{i}^{2}}right)}right)}^{{tau E}_{it}^{*}}}times {{left({varepsilon }_{0,im}{e}^{left(-frac{{d}_{im}^{2}}{2{sigma }_{i}^{2}}right)}right)}^{1-{E}_{it}^{*}}}$$
(7)
where ε0,im is the maximum nightly probability of encounter for device m, or the probability if device m was placed at the center of the home range of rat i. The variable σi is the standard deviation from Eq. (1) (i.e., σi is estimated jointly from the telemetry and encounter data) and dim is the distance (in m) between the home range center of rat i and device m; only devices within a distance of 3.72σi from the home range center were considered in the calculation in Eq. (7)70. Finally, τ is a strictly positive parameter (i.e., τ  > 0), measuring the degree of device-shyness, which is multiplied by an indicator variable (left({E}_{it}^{*}right)) which takes a value of 0 when individual i has not encountered a device (of any type) on nights prior to night t, or a value of 1 if it had previously encountered one, regardless of the type of device it encountered. If τ  1 then rats are ‘device-shy’ and thus more likely to avoid devices on nights following an initial encounter. ({E}_{it}^{*}) was reset to 0 after 20 days of no encounters with a device. Following65 we set the prior on τ as Gamma(0.933, 8.33) (shape and rate parameters, respectively).Values of ε0,im were predicted as a function of σi, device type, and individual effects using the following equation:$$logitleft({varepsilon }_{0, im}right)={alpha }_{0}+ {alpha }_{1}mathrm{ln}left({sigma }_{i}right)+ {alpha }_{2}{chewcard}_{m}+{alpha }_{3}{waxtag}_{m}+{delta }_{i}$$
(8)
where α2 and α3 quantify the increase or decrease in the maximal probability of encountering a chew card or a WaxTag relative to a bait station (which is the reference category). The δi parameters account for individual differences in ε0. Finally, we allowed ε0 to be a function of ln(σi) because we assumed encounter probability at home range center will decrease with increasing home range size (as suggested by71 and shown by65). The priors on the α coefficients and δ were Normal(0, 10) and Normal(0, 1), respectively.The interaction data (Iimn) across all devices m and nights n when encounters occurred was modelled as a Bernoulli process with probability θ, which was a function of device type and individual effects:$${mathrm{I}}_{imn}sim Bernoullileft({theta }_{imn}right)$$
(9)
$$logitleft({theta }_{imn}right)={lambda }_{0}+ {lambda }_{1}{chewcard}_{m}+{lambda }_{2}{waxtag}_{m}+{lambda }_{3}{I}_{in}^{*}+{rho }_{i}$$
(10)
where θimn is the probability of rat i interacting with device m given that it has encountered it on night n, and λ1 and λ2 quantify the increase or decrease in the conditional probability of interaction for a chew card or a WaxTag relative to a bait station. The λ3 parameter is analogous to τ in Eq. (7) but for the process of interaction given encounter with a device. However, by incorporating λ3 directly into a linear equation, this parameter can take negative values and thus should be interpreted differently to τ: if λ3  0 indicates that individuals become ‘device-happy’ after an initial interaction. This parameter is multiplied by an indicator variable ({(I}_{in}^{*})) which takes a value of 0 when individual i has not interacted with a device (of any type) on nights prior to night n, or a value of 1 when it has interacted with one previously, regardless of the type of device it interacted with. If a rat had not interacted with a device for 20 days, ({I}_{in}^{*}) was reset to 0. Finally, the ρi parameters account for individual differences in θ. The priors on the λ coefficients and ρ were Normal(0, 10) and Normal(0, 1), respectively. Although we explicitly modelled spatial autocorrelation in the probability of encountering a device, we did not do so for the probability of interaction given an encounter. In this instance we assumed that whether an animal chose to interact with an encountered device would depend on its previous experience (as quantified by λ3) rather than the spatial location of nearby devices.We used Markov Chain Monte Carlo (MCMC) simulation to estimate model parameters using Python programming language. The variance parameter V was sampled from the full conditional posteriors, but all other parameters were estimated using the Metropolis algorithm69. Posterior summaries were taken from four chains containing 3000 samples each (with a burn-in of 2000 and a thinning rate of 30). Convergence on posteriors was assessed by visual inspection and a scale reduction factor  More

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Sampling location and sediment coringSamples were collected during IODP Exp. 382 ‘Iceberg Alley and Subantarctic Ice and Ocean Dynamics’ on-board RV Joides Resolution between 20 March and 20 May 2019. Specifically, we collected samples at Site U1534 (Falkland Plateau, 606 m water depth), U1536 (Dove Basin, Scotia Sea, 3220 m water depth), and Site U1538 (Pirie Basin, Scotia Sea, 3130 m water depth) (Fig. 1). Site U1534 is located at the Subantarctic Front on a contourite drift at the northern limit of the Scotia Sea. This setting is ideal to study the poorly understood role of Antarctic Intermediate Water (AAIC) and its impact on the Atlantic Meridional Overturning Circulation (AMOC) along the so-called ‘cold water route’ that connects to the Pacific Ocean through the Drake Passage, as opposed to the ‘warm water route’ that connects to the Indian Ocean via the Agulhas Current42. Sites U1536 and U1538 are located in the southern and central Scotia Sea, respectively, and were drilled to study the Neogene flux of icebergs through ‘Iceberg Alley’, the main pathway along which icebergs calved from the margin of the AIS travel as they move equatorward into the warmer waters of the Antarctic Circumpolar Current (ACC)23. sedaDNA samples collected at Site U1534 were from Hole C, at Site U1536 from Hole B, and at Site U1538 from Holes C and D (Table 1), and in the following we refer to site names only. IODP Expedition proposals undergo a rigorous environmental protection and safety review, which is approved by the IODP’s Environmental Protection and Safety Panel (EPSP) and/or the Safety Panel. The same procedure was applied to IODP Exp. 382 and approval was provided by the EPSP. Sediment samples for sedaDNA analyses were imported to Australia under Import Permit number 0002658554 provided by the Australian Government Department for Agriculture and Water Resources (date of issue: 19 September 2018), and were stored and extracted at a quarantine approved facility (AA Site No. S1253, Australian Centre for Ancient DNA). No ethical approval was required for this study.Table 1 Sampling location and sample detailsFull size tableSample age determinationAge control for Site U1534 is based on tuning of benthic foraminifera δ18O to the LR04 stack43. Wherever present specimens of Uvigerina bifurcata were picked from samples at 10 cm intervals. During warmer periods when U. bifurcata was not present, Melonis affinis and/or Hoeglundina elegans were analysed. Sedimentation rates over the intervals sampled for sedaDNA typically range between 6 and 30 cm/kyr, with rates exceeding 100 cm/kyr during the Last Glacial Maximum ~20,000 years ago (20 ka). For our deepest sample, U1534C-10H-6_115cm (90.95 mbsf), we only have biostratigraphically assigned ages available (shipboard data), which date this sample as early Pleistocene (~2.5–0.7 million years ago, Ma44).Low-resolution age control for both Sites U1536 and U1538 was established using shipboard magneto- and biostratigraphy21,23. Average sedimentation rates are ~10 cm/kyr for Site U1536, with elevated values (up to 20 cm/kyr) in the upper ~80 mbsf (the last ~400 ka). Site U1538 average sedimentation rates are twice as high, averaging ~20 cm/kyr. Especially in the upper ~430 mbsf (the last 1.8 Ma), rates are up to 40 cm/kyr. Higher resolution age models are based on dust climate couplings, correlating sedimentary dust proxy records such as magnetic susceptibility and sedimentary Ca and Fe records to ice-core dust proxy records over the last 800 ka45 and to a benthic isotopic stack26 before that. These age models were established for Site U1537 (adjacent to Site U1536) and provide orbital to millennial scale resolution. For this study we correlated sedimentary cycles of Sites U1536 and U1538 to U1537 to achieve similar resolution and to be able to determine if a sample originates from a glacial or interglacial period (Table 1).Sampling of sedaDNAA detailed description of sedaDNA sampling methods can be found in ref. 24. In brief, we used advanced piston coring (APC) to acquire sediment cores, which recovers the least disturbed sediments46,47,48 and is thus the preferred technique for sedaDNA sampling. All samples were taken on the ship’s ‘catwalk’, where, once the core was on deck, the core liners were wiped clean twice (3% sodium hypochlorite, ‘bleach’) at each cutting point. Core cutting tools were sterilised before each cut (3% bleach and 80% ethanol) of the core in 1 m sections. The outer ~3 mm of surface material were removed from the bottom of each core section to be sampled, using sterilised scrapers (~4 cm wide; bleach and ethanol treated). A cylindrical sample was taken from the core centre using a sterile (autoclaved) 10 mL cut-tip syringe, providing ~5 cm3 of sediment material. The syringe was placed in a sterile plastic bag (Whirl-Pak) and immediately frozen at −80 °C. The mudline (sediment/seawater interface) was transferred from the core liner into a sterile bucket (3% bleach treated), and 10 mL sample was retained in a sterile 15 mL centrifuge tube (Falcon) and frozen at −80 °C. Samples were collected at various depth intervals depending on the site to span the Holocene up to ~1 million years (Table 1). This lower depth/age limit was determined by switching coring system from APC to the extended core barrel (XCB) system.To test for potential airborne contamination, at least one air control was taken during the sedaDNA sampling process per site. For this, an empty syringe was held for a few seconds in the sampling area and then transferred into a sterile plastic bag and frozen at −80 °C. The air controls were processed, sequenced and analysed alongside the sediment samples.Contamination control using perfluoromethyldecalin tracersAs part of the APC process, drill fluid (basically, seawater) is pumped into the borehole to trigger the hydraulic coring system, therefore, the potential for contamination exists due to drill fluid making contact with the core liner. To assess the latter, we added the non-toxic chemical tracer perfluoromethyldecalin (PFMD) to the drill fluid at a rate of ~0.55 mL min−1 for cores collected at Sites U1534 and U153649. As we found that PFMD concentrations were very low at these sites (Results section), the infusion rate was doubled prior to sedaDNA sampling at Site U1538 to ensure low PFMD concentrations represent low contamination and not delivery failure of PFMD to the core. At each sedaDNA sampling depth, one PFMD sample was taken from the periphery of the core (prior to scraping, to test whether drill fluid reached the core pipe), and one next to the sedaDNA sample in the centre of the core (after scraping, to minimise differences to the sedaDNA sample, and testing if drill fluid had reached the core centre). We transferred ~3 cm3 of sediment using a disposable, autoclaved 5 mL cut-tip syringe into a 20 mL headspace vial with metal caps and Teflon seals. We also collected a sample of the tracer-infused drill fluid at each site, by transferring ~10 mL of the fluid collected at the injection pipe on the rig floor via a sterile plastic bottle into a 15 mL centrifuge tube (inside a sterile plastic bag) and freezing it at −80 °C. These drill fluid controls were processed and analysed in the same way as the sedaDNA samples including sequencing. Samples were analysed using gas chromatography (GC-µECD; Hewlett-Packard 6890).A detailed description of the PFMD GC measurements is provided in ref. 24. Briefly, PFMD measurements were undertaken in batches per site for U1534, U1536 and U1538. This included the analyses of PFMD samples collected at two additional holes at these sites, U1534D and U1536C, from which we also collected sedaDNA samples but that are not part of this study. PFMD is categorised as the stereoisomers of PFMD (C11F20), which add up to 87-88% (and with the remaining 12% being additional perfluoro compounds unable to be separated by the manufacturer). We exclusively refer to the first and measurable PFMD category, calibrating for the 88% in bottle concentrations during concentration calculations. Each GC analysis run included the measurement of duplicate blanks and duplicate PFMD standards. Due to a large sample number, PFMD at Site U1538 was measured in three separate runs, with the first and last run including triplicate blank and triplicate PFMD standards (duplicates in the second run), and the last run also containing a drill-fluid sample. To blank-correct PFMD concentrations, we subtracted the average PFMD concentration of all blanks per run from PFMD measurements in that run. To determine the detection limit of PFMD, we used three times the standard deviation of the average blank PFMD values per run; due to all blank values for the U1538 runs being 0, we used three times the standard deviation of the lowest PFMD standard for this site in this calculation. This provided us with a PFMD detection limit of 0.2338 ng mL−1. Any PFMD measurements of samples below this limit were rejected.
sedaDNA extractions and metagenomic library preparationsA total of 80 sedaDNA extracts and metagenomic shotgun libraries (Table 1) were prepared following8,10. For the sedaDNA extractions, we randomised our samples and controls and extracted sedaDNA in batches of 16 extracts/libraries at a time, with each batch including at least one air control and one extraction blank control (EBC), and the last batch including mudline and PFMD samples to avoid contamination of the sedaDNA samples. In brief, we used 20 µL sedaDNA extracts in a repair reaction (using T4 DNA polymerase, New England Biolabs, USA; 15 min, 25 °C), then purified the sedaDNA (MinElute Reaction Cleanup Kit, Qiagen, Germany), ligated adaptors (T4 DNA ligase, Fermentas, USA, where truncated Illumina-adaptor sequences containing two unique 7 base-pair (bp) barcodes were attached to the double-stranded DNA; 60 min, 22 °C), purified the sedaDNA again (MinElute Reaction Cleanup Kit, Qiagen), and then added a fill-in reaction with adaptor sequences (Bst DNA polymerase, New England Biolabs, USA; 30 min, 37 °C, with polymerase deactivation for 10 min, 80 °C). We amplified the barcoded libraries using IS7/IS8 primers50 (8 replicates per sample, where each replicate was a 25 µL reaction containing 3 µL DNA template; using 22 cycles), purified (AxyPrep magnetic beads, Axygen Biosciences, USA; 1:1.8 library:beads) and quantified them (Qubit dsDNA HS Assay, Invitrogen, Molecular Probes, USA). We amplified the libraries (8 replicates per sample, 13 amplification cycles) using IS4 and GAII Indexing Primers50, purified (AxyPrep magnetic beads, at a ratio of 1:1.1 library:beads), quantified and quality-checked using Qubit (dsDNA HS Assay, Invitrogen, USA) and TapeStation (Agilent Technologies, USA). We combined the libraries into an equimolar pool (volume of 68 µL in total), diluted this pool with nuclease-free H2O to 100 µL, and performed a ‘reverse’ AxyPrep clean-up to retain only the small DNA fragments typical for ancient DNA (≤ 500 bp; initial library:beads ratio of 1:0.6, followed by 1:1.1, and double-eluted in 30 µL nuclease-free H2O8,51). We added one more AxyPrep clean-up to remove primer-dimer (library:beads ratio of 1:1.05) and checked sedaDNA quantity and quality via TapeStation and qPCR (QuantStudio, Applied Biosystems, USA). The libraries sequenced at the Garvan Institute for Medical Research, Sydney, Australia (Illumina NovaSeq 2 × 100 bp).
sedaDNA data processingThe sequencing data was processed and filtered as described in detail in refs. 8, 10. Briefly, data filtering involved the removal of sequences More

For several years, systematic research has been carried out on the pollution of the night sky by artificial light in the city of Toruń11,36,38. The main objective is to monitor this phenomenon, including its spatial and temporal variability and the most important factors affecting it. Based on previous experience, an in-house measurement device was constructed to automate the process of data acquisition.Genesis of the projectThe first measurements pertaining to the phenomenon of night sky pollution in Toruń were made in autumn 2017, followed by regular observations using a handheld SQM photometer (Unihedron, Canada) as part of a project implemented in 2017–2018. To this end, a permanent measurement network distributed throughout the city was established, consisting of 24 locations. During a one night measurement session taking place during the astronomical night (there is no such period at the latitude of Toruń in the summer), sky brightness was measured at all sites. The results of spot monitoring were plotted using interpolation methods and visualisation tools available in GIS systems11,39, which helped to determine the spatial distribution and extent of night sky light pollution. The intensity of this phenomenon at each of the surveyed points was also explored in relation to the distinguished landcover categories and types of urban development.Repeatable measurements performed regularly over such a long period of time were characterised by significant limitations. One measurement session was very time-consuming, as it lasted about two hours, during which time all measurement locations were visited, covering a distance of almost 50 km each night by car. Despite the observance of all time frames and sticking to the plan of fieldwork, measurements were not carried out simultaneously at all the locations, which affected the results, especially during the night with changing cloud cover. Although the measurements were carried out with great consistency and care, they were performed in a spatial buffer of about 5 m, which could unintentionally slightly affect the obtained results. An additional limitation was also a one-time night measurement at one point, instead of a whole series of measurements at specific time intervals. Inaccuracies in the readings within a single session could have been caused by sudden changes in meteorological parameters. In the adopted procedure, it was not possible to carry out simultaneous measurements in identical time and weather conditions at all the locations, not to mention the involvement of the personnel in each tour of the measurement network points.Using the experience gained and after an analysis of the identified constraints and the technical capabilities at hand, work began in 2019 on developing a network for automatic remote monitoring of light pollution of the night sky in Toruń, based on designed in-house recording devices.Design, functional and utility features of the deviceTo enhance the research on light pollution in urban space, work has begun on the construction of a device that would perform automatic measurements, would be mobile, battery-powered and use long-range wireless communication. All the aforementioned features are in line with the strategy of Industry 4.0 and modern solutions proposed as part of the Smart City concept.The concept of Industry 4.0 assumes the more and more common use of process automation as well as the processing and exchange of data with the use of new transmission technologies26. LoRaWAN is one of the solutions used for communication of Internet of Things (IoT) devices, which supports the development of Smart Cities in the Smart Environment area. As a result, the interactivity, frequency, and scope of measurements carried out in urbanized areas are increased40,41.According to the developed project, the device was to serve as a meter of very low intensity light observed in the night sky. In this respect, it was necessary to use a sensor with technical parameters suitable for very accurate measurements of light intensity. To locally verify the weather conditions occurring during the operation of the device, it was decided to carry out additional simultaneous measurements of other environmental parameters—temperature and moisture content. The analysis of the spatial coverage of the study area indicated that 36 measurement devices should be deployed to provide full coverage of Toruń. The concept of creating an urban measurement network assumes the selection of points covering the whole city relatively evenly and representing different types of housing development and elements of land cover. It was assumed that measurements will be made only at night, between 21 p.m. and 6 a.m. on the following day, at 15 min intervals, and in addition, weather conditions will be recorded twice a day.Construction and technical parameters of the device, and selected characteristics of its componentsA prototype device meeting all the predefined functionalities was constructed based on available electronic modules. The B-L072Z-LRWAN Discovery developmental board from STMicroelectronics42 was selected as the main electronic component providing wireless communication. This board has an integrated LoRa communication module, enabling low-power wireless messaging, and also allows the board to enter a low-power state during hibernation, and thus target long-term battery-powered operation. This module is fully programmable, which enables future expansion of the set with other functionalities. The TSL2591 light sensor from AMS, which has high sensitivity and registration accuracy, was selected as a component implementing the light intensity measurement. Its great advantage is a wide measurement range of 188 μlx to 88 000 lx, sensitivity reaching 0.000377 lx, and a wide dynamic range (WDR) of 600 M:143. The sensor used has two diodes with different spectral properties. One of them registers visible light together with infrared (in the range from 400 to 1 100 nm), while the other is responsible for the registration of infrared light (between 500 and 1 100 nm). Thanks to this solution, we can use the results in various ways. The use of the formula provided by the manufacturer allows us to obtain spectral characteristics similar to the human eye. The presence of a compensating diode makes a difference compared to the sensor used in the SQM device, so the results obtained in the measurements may be slightly different.To measure additional environmental parameters, the X-NUCLEO-IKS01A2 development board from STMicroelectronics was used, which is connected to the STM32 microcontroller via the I2C interface44. This board enables the recording of a number of parameters, however, in the constructed device it is only responsible for reading the temperature and humidity of the environment. This results from the necessity to limit the size of the message packets sent, while at the same time improving the operating range and reducing the power consumption of the device.Once all the components had been selected, tested and integrated, the process of final connection and programming was carried out. The base of the device, i.e. the B-L072Z-LRWAN development board was connected to the X-NUCLEO-IKS01A2 environmental sensor board, using Arduino connectors. Using standard wires, a TSL2591 light sensor was added by connecting the corresponding I2C (SCL and SDA), power supply (VIN), sensor ground (GND) pins and the X-NUCLEO-IKS01A2 board.All components used were placed in a standard external casing with dimensions of 8.0 × 5.4 × 15.8 cm. In its lower part an opening was made for an external antenna, while in the upper part a specifically selected opening was cut out, protected with a glass pane, through which measurements are performed by the light sensor (Fig. 2).Figure 2(photo by Dominika Karpińska).Constructed device viewFull size imageFollowing the above steps, an automatic device was constructed to record light intensity in the lower troposphere, i.e. to measure the pollution of the night sky by artificial light coming from the Earth’s surface. Selected technical parameters of the device are presented in Table 1.Table 1 Selected technical parameters of the device for measuring light pollution of the night sky.Full size tableFlowchart of the system operationAfter constructing the device and writing the control software, the construction of the entire measurement system was started. Each of the measuring instruments is ultimately connected to the communication gateway using LoRa technology. A MultiTech communication gateway with a LoRaWAN module was used as an access device. To successfully connect the gateway to the measuring device, it was necessary to configure the communication gateway software. To this end, the information about the unique device number (Dev EUI) and the application key and its number (App EUI and App Key) was used. Once the unit is configured, it is possible to send data to the communication gateway and read them using NodeRED, a programming tool where data are redirected to a selected server, which stores all measurement results. Figure 3 shows a schematic representation of the constructed measurement system.Figure 3Schematic diagram of the measurement system.Full size image More