Philippa Kaur

Kilpatrick, A. M. et al. Lyme disease ecology in a changing world: consensus, uncertainty and critical gaps for improving control. Philos. Trans. R. Soc. B-Biol. Sci. 372, 15. https://doi.org/10.1098/rstb.2016.0117 (2017).Article 

Google Scholar 
Adenubi, O. T. et al. Pesticidal plants as a possible alternative to synthetic acaricides in tick control: A systematic review and meta-analysis. Ind. Crop. Prod. 123, 779–806. https://doi.org/10.1016/j.indcrop.2018.06.075 (2018).CAS 
Article 

Google Scholar 
Jordan, R. A. & Schulze, T. L. Availability and nature of commercial tick control services in three Lyme disease endemic states. J. Med. Entomol. 57, 807–814. https://doi.org/10.1093/jme/tjz215 (2019).CAS 
Article 

Google Scholar 
Isman, M. B. Botanical insecticides in the twenty-first century – Fulfilling their promise?. Ann. Rev. Entomol. 65, 233–249 (2020).CAS 
Article 

Google Scholar 
Eisen, L. Control of ixodid ticks and prevention of tick-borne diseases in the United States: The prospect of a new Lyme disease vaccine and the continuing problem with tick exposure on residential properties. Ticks Tick-Borne Dis. https://doi.org/10.1016/j.ttbdis.2021.101649 (2021).Article 
PubMed 
PubMed Central 

Google Scholar 
Santos, A. C. C. et al. Apis mellifera (Insecta: Hymenoptera) in the target of neonicotinoids: A one-way ticket? Bioinsecticides can be an alternative. Ecotoxicol. Environ. Safe. 163, 28–36. https://doi.org/10.1016/j.ecoenv.2018.07.048 (2018).CAS 
Article 

Google Scholar 
Matos, W. B. et al. Potential source of ecofriendly insecticides: Essential oil induces avoidance and cause lower impairment on the activity of a stingless bee than organosynthetic insecticides, in laboratory. Ecotoxicol. Environ. Safe. 209, 111764. https://doi.org/10.1016/j.ecoenv.2020.111764 (2021).CAS 
Article 

Google Scholar 
Gashout, H. A., Guzman-Novoa, E., Goodwin, P. H. & Correa-Benítez, A. Impact of sublethal exposure to synthetic and natural acaricides on honey bee (Apis mellifera) memory and expression of genes related to memory. J. Insect Physiol. 121, 104014. https://doi.org/10.1016/j.jinsphys.2020.104014 (2020).CAS 
Article 
PubMed 

Google Scholar 
Eisen, L. & Dolan, M. C. Evidence for personal protective measures to reduce human contact with Blacklegged ticks and for environmentally based control methods to suppress host-seeking Blacklegged ticks and reduce infection with Lyme disease spirochetes in tick vectors and rodent reservoirs. J. Med. Entomol. 53, 1063–1092. https://doi.org/10.1093/jme/tjw103 (2016).Article 
PubMed 

Google Scholar 
Dyer, M. C., Requintina, M. D., Berger, K. A., Puggioni, G. & Mather, T. N. Evaluating the effects of minimal risk natural products for control of the tick, Ixodes scapularis (Acari: Ixodidae). J. Med. Entomol. 58, 390–397. https://doi.org/10.1093/jme/tjaa188 (2021).CAS 
Article 
PubMed 

Google Scholar 
Schulze, T. L. & Jordan, R. A. Synthetic pyrethroid, natural product, and entomopathogenic fungal acaricide product formulations for sustained early season suppression of host-seeking Ixodes scapularis (Acari: Ixodidae) and Amblyomma americanum nymphs. J. Med. Entomol. 58, 814–820. https://doi.org/10.1093/jme/tjaa248 (2021).CAS 
Article 
PubMed 

Google Scholar 
Bharadwaj, A., Stafford, K. C. & Behle, R. W. Efficacy and environmental persistence of nootkatone for the control of the Blacklegged tick (Acari: Ixodidae) in residential landscapes. J. Med. Entomol. 49, 1035–1044. https://doi.org/10.1603/me11251 (2012).Article 
PubMed 

Google Scholar 
Pavela, R. & Sedlák, P. Post-application temperature as a factor influencing the insecticidal activity of the essential oil from Thymus vulgaris. Ind. Crop. Prod. 113, 46–49 (2018).CAS 
Article 

Google Scholar 
Brunner, J. L., Killilea, M. & Ostfeld, R. S. Overwintering survival of nymphal Ixodes scapularis (Acari: Ixodidae) under natural conditions. J. Med. Entomol. 49, 981–987. https://doi.org/10.1603/me12060 (2012).Article 
PubMed 

Google Scholar 
Chown, S. L. & Nicolson, S. W. Insect Physiol. Ecol. (Oxford University Press, 2004).Ogden, N. H., Beard, C. B., Ginsberg, H. S. & Tsao, J. I. Possible effects of climate change on Ixodid ticks and the pathogens they transmit: Predictions and observations. J. Med. Entomol. 58, 1536–1545 (2021).Article 

Google Scholar 
Ballard, K. & Bone, C. Exploring spatially varying relationships between Lyme disease and land cover with geographically weighted regression. Appl. Geo. 127, 102383 (2021).Article 

Google Scholar 
Neelakanta, G., Sultana, H., Fish, D., Anderson, J. F. & Fikrig, E. Anaplasma phagocytophilum induces Ixodes scapularis ticks to express an antifreeze glycoprotein gene that enhances their survival in the cold. J. Clin. Invest. 120, 3179–3190. https://doi.org/10.1172/jci42868 (2010).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Adamo, S. A. Animals have a Plan B: how insects deal with the dual challenge of predators and pathogens. J. Comp. Physiol. B-Biochem. Syst. Environ. Physiol. 190, 381–390. https://doi.org/10.1007/s00360-020-01282-5 (2020).Article 

Google Scholar 
Adamo, S. A. How insects protect themselves against combined starvation and pathogen challenges, and the implications for reductionism. Comp. Biochem. Physiol. B-Biochem. Molec. Biol. https://doi.org/10.1016/j.cbpb.2021.110564 (2021).Article 

Google Scholar 
Linske, M. A. et al. Impacts of deciduous leaf litter and snow presence on nymphal Ixodes scapularis (Acari: Ixodidae) overwintering survival in coastal New England, USA. Insects https://doi.org/10.3390/insects10080227 (2019).Article 
PubMed 
PubMed Central 

Google Scholar 
Burtis, J. C., Fahey, T. J. & Yavitt, J. B. Survival and energy use of Ixodes scapularis nymphs throughout their overwintering period. Parasitol. 146, 781–790. https://doi.org/10.1017/s0031182018002147 (2019).Article 

Google Scholar 
Boehnke, D., Gebhardt, R., Petney, T. & Norra, S. On the complexity of measuring forests microclimate and interpreting its relevance in habitat ecology: the example of Ixodes ricinus ticks. Parasit. Vectors 10, 549. https://doi.org/10.1186/s13071-017-2498-5 (2017).Article 
PubMed 
PubMed Central 

Google Scholar 
Lindsay, L. R. et al. Survival and development of Ixodes scapularis (Acari, Ixodidae) under various climatic conditions in Ontario, Canada. J. Med. Entomol. 32, 143–152. https://doi.org/10.1093/jmedent/32.2.143 (1995).CAS 
Article 
PubMed 

Google Scholar 
Lindsay, L. R. et al. Survival and development of the different life stages of Ixodes scapularis (Acari: Ixodidae) held within four habitats on Long Point, Ontario, Canada. J. Med. Entomol. 35, 189–199. https://doi.org/10.1093/jmedent/35.3.189 (1998).CAS 
Article 
PubMed 

Google Scholar 
Ginsberg, H. S. et al. Woodland type and spatial distribution of nymphal Ixodes scapularis (Acari: Ixodidae). Environ. Entomol. 33, 1266–1273. https://doi.org/10.1603/0046-225x-33.5.1266 (2004).Article 

Google Scholar 
Clow, K. M. et al. The influence of abiotic and biotic factors on the invasion of Ixodes scapularis in Ontario, Canada. Ticks Tick-Borne Dis. 8, 554–563. https://doi.org/10.1016/j.ttbdis.2017.03.003 (2017).Article 
PubMed 

Google Scholar 
Natural Resources Canada. Balsam fir, (2015).Khatchikian, C. E. et al. Recent and rapid population growth and range expansion of the Lyme disease tick vector, Ixodes scapularis North America. Evolution 69, 1678–1689. https://doi.org/10.1111/evo.12690 (2015).Article 
PubMed 
PubMed Central 

Google Scholar 
Pichette, A., Larouche, P. L., Lebrun, M. & Legault, J. Composition and antibacterial activity of Abies balsamea essential oil. Phytotherapy Res. 20, 371–373 (2006).CAS 
Article 

Google Scholar 
Poaty, B., Lahlah, J., Porqueres, F. & Bouafif, H. Composition, antimicrobial and antioxidant activities of seven essential oils from the North American boreal forest. World J. Microbiol. Biotechnol. 31, 907–919. https://doi.org/10.1007/s11274-015-1845-y (2015).CAS 
Article 
PubMed 

Google Scholar 
Beasley, T. M. & Schumacker, R. E. Multiple regression approach to analyzing contingency tables: Post hoc and planned comparison procedures. J. Exp. Ed. 64, 79–93. https://doi.org/10.1080/00220973.1995.9943797 (1995).Article 

Google Scholar 
Canon, L., Deslauriers, A., Mshvildadze, V. & Pichette, A. Volatile compounds in the foliage of balsam fir analyzed by static headspace gas chromotography (HS-GS): An example of the spruce budworm defoliation effect in the boreal forest of Quebec, Canada. Microchem. J. 110, 587–590 (2013).Article 

Google Scholar 
Faraone, N., MacPherson, S. & Hillier, N. K. Behavioral responses of Ixodes scapularis tick to natural products: development of novel repellents. Exp. Appl. Acarol. 79, 195–207. https://doi.org/10.1007/s10493-019-00421-0 (2019).CAS 
Article 
PubMed 

Google Scholar 
McMillan, L. E., Miller, D. W. & Adamo, S. A. Eating when ill is risky: immune defense impairs food detoxification in the caterpillar Manduca sexta. J. Exp. Biol. 221, 173336 (2018).
Google Scholar 
Gazave, E., Chevillon, C., Lenormand, T., Marquine, M. & Raymond, M. Dissecting the cost of insecticide resistance genes during the overwintering period of the mosquito Culex pipiens. Heredity 87, 441–448 (2001).CAS 
Article 

Google Scholar 
Lalouette, L., Williams, C. M., Hervant, F., Sinclair, B. J. & Renault, D. Metabolic rate and oxidative stress in insects exposed to low temperature thermal fluctuations. Comp. Biochem. Physiol. A 158, 229–234 (2011).CAS 
Article 

Google Scholar 
Clark, D. D. Lower temperature limits for activity of several Ixodid ticks: Effects of body size and rate of temperature change. J. Med. Entomol. 32, 449–452 (1995).CAS 
Article 

Google Scholar 
Carroll, J. F. & Kramer, M. Winter activity of Ixodes scapularis (Acari : Ixodidae) and the operation of deer-targeted tick control devices in Maryland. J. Med. Entomol. 40, 238–244. https://doi.org/10.1603/0022-2585-40.2.238 (2003).CAS 
Article 
PubMed 

Google Scholar 
Ginsberg, H. S. et al. Environmental factors affecting survival of immature Ixodes scapularis and implications for geographical distribution of Lyme disease: the climate/behavior hypothesis. PLoS ONE 12, e0168723. https://doi.org/10.1371/journal.pone.0168723 (2017).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Quadros, D. G., Johnson, T. L., Whitney, T. R., Oliver, J. D. & Chavez, A. S. O. Plant-derived natural compounds for tick pest control in livestock and wildlife: Pragmatism or utopia?. Insects https://doi.org/10.3390/insects11080490 (2020).Article 
PubMed 
PubMed Central 

Google Scholar 
Ogendo, J. et al. Biocontrol potential of selected plant essential oil constituents as fumigants of insect pests attacking stored food commodities. Health 10, 287–318 (2011).
Google Scholar 
Panella, N. A., Karchesy, J., Maupin, G. O., Malan, J. C. & Piesman, J. Susceptibility of immature Ixodes scapularis (Acari: Ixodidae) to plant-derived acaricides. J. Med. Entomol. 34, 340–345 (1997).CAS 
Article 

Google Scholar 
Rosado-Aguilar, J. A. et al. Plant products and secondary metabolites with acaricide activity against ticks. Vet. Parasitol. 238, 66–76. https://doi.org/10.1016/j.vetpar.2017.03.023 (2017).CAS 
Article 
PubMed 

Google Scholar 
Jaenson, T. G. T., Carboui, S. & Palsson, K. Repellency of oils of lemon eucalyptus, geranium, and lavender and the mosquito repellent MyggA natural to Ixodes ricinus (Acari : Ixodidae) in the laboratory and field. J. Med. Entomol. 43, 731–736. https://doi.org/10.1603/0022-2585(2006)43[731:Rooole]2.0.Co;2 (2006).CAS 
Article 
PubMed 

Google Scholar 
Eigbrett, C. Natural Sourcing Organic Essential Oils Oxford, Connecticut, USA, .praannaturals.com/downloads/msds/SDS_Organic_Essential_Oil_Fir_Balsam_Canada.pdf (2016).Schulze, T. L. et al. Efficacy of granular deltamethrin against Ixodes scapularis and Amblyomma americanum (Acari: Ixodidae) nymphs. J. Med. Entomol. 38, 344–346. https://doi.org/10.1603/0022-2585-38.2.344 (2001).CAS 
Article 
PubMed 

Google Scholar 
Elias, S. P. et al. Effect of a botanical acaricide on Ixodes scapularis (Acari: Ixodidae) and nontarget arthropods. J. Med. Entomol. 50, 126–136. https://doi.org/10.1603/me12124 (2013).Article 
PubMed 

Google Scholar 
Burtis, J. C., Yavitt, J. B., Fahey, T. J. & Ostfeld, R. S. Ticks as soil-dwelling arthropods: an intersection between disease and soil ecology. J. Med. Entomol. 56, 1555–1564. https://doi.org/10.1093/jme/tjz116 (2019).Article 
PubMed 

Google Scholar 
Burtis, J. C., Ostfeld, R. S., Yavitt, J. B. & Fahey, T. J. The relationship between soil arthropods and the overwinter survival of Ixodes scapularis (Acari: Ixodidae) under manipulated snow cover. J. Med. Entomol. 53, 225–229. https://doi.org/10.1093/jme/tjv151 (2016).CAS 
Article 
PubMed 

Google Scholar 
Guerra, M. et al. Predicting the risk of Lyme disease: Habitat suitability for Ixodes scapularis in the north central United States. Emerg. Infect. Dis. 8, 289–297. https://doi.org/10.3201/eid0803.010166 (2002).Article 
PubMed 
PubMed Central 

Google Scholar 
Bunnell, J. E., Price, S. D., Das, A., Shields, T. M. & Glass, G. E. Geographic information systems and spatial analysis of adult Ixodes scapularis (Acari: Ixodidae) in the Middle Atlantic region of the USA. J. Med. Entomol. 40, 570–576. https://doi.org/10.1603/0022-2585-40.4.570 (2003).Article 
PubMed 

Google Scholar 
Lubelczyk, C. B. et al. Habitat associations of Ixodes scapularis (Acari: Ixodidae) in Maine. Environ. Entomol. 33, 900–906. https://doi.org/10.1603/0046-225x-33.4.900 (2004).Article 

Google Scholar 
Killilea, M. E., Swei, A., Lane, R. S., Briggs, C. J. & Ostfeld, R. S. Spatial dynamics of Lyme disease: A review. EcoHealth 5, 167–195. https://doi.org/10.1007/s10393-008-0171-3 (2008).Article 
PubMed 

Google Scholar 
Stafford, K. C. Survival of immature Ixodes scapularis (Acari: Ixodidae) at different relative humidities. J. Med. Entomol. 31, 310–314 (1994).Article 

Google Scholar 
Bertrand, M. R. & Wilson, M. L. Microclimate-dependent survival of unfed adult Ixodes scapularis (Acari: Ixodidae) in Nature: Life cycle and study design implications. J. Med. Entomol. 33, 619–627 (1996).CAS 
Article 

Google Scholar 
Lindsay, L. R. et al. Microclimate and habitat in relation to Ixodes scapularis (Acari: Ixodidae) populations on Long Point, Ontario, Canada. J. Med. Entomol. 36, 255–262. https://doi.org/10.1093/jmedent/36.3.255 (1999).CAS 
Article 
PubMed 

Google Scholar 
Thompson, C., Spielman, A. & Krause, P. J. Coinfecting deer-associated zoonoses: Lyme disease, babesiosis, and ehrlichiosis. Clin. Infect. Dis. 33, 676–685 (2001).CAS 
Article 

Google Scholar 
Hinckley, A. F. et al. effectiveness of residential acaricides to prevent Lyme and other tick-borne diseases in humans. J. Infect. Dis. 214, 182–188. https://doi.org/10.1093/infdis/jiv775 (2016).Article 
PubMed 

Google Scholar 
Keesing, F. et al. Effects of Ttck-control interventions on tick abundance, human encounters with Ttcks, and incidence of tickborne diseases in residential neighborhoods, New York, USA. Emerg. Infect. Dis. 28, 957–966. https://doi.org/10.3201/eid2805.211146 (2022).Article 
PubMed 
PubMed Central 

Google Scholar 
Hayes, L. E., Scott, J. A. & Stafford, K. C. Influences of weather on Ixodes scapularis nymphal densities at long-term study sites in Connecticut. Ticks Tick-Borne Dis. 6, 258–266. https://doi.org/10.1016/j.ttbdiS.2015.01.006 (2015).Article 
PubMed 

Google Scholar 
Rand, P. W. et al. Trial of a minimal-risk botanical compound to control the vector tick of Lyme disease. J. Med. Entomol. 47, 695–698 (2010).CAS 
Article 

Google Scholar 
United Nations. Convention on Biological Diversity. (1992).Convention on International Trade in Endangered Species of Wild Fauna and Flora. (1973).Burtis, J. C. Method for the efficient deployment and recovery of Ixodes scapularis (Acari: Ixodidae) nymphs and engorged larvae from field microcosms. J. Med. Entomol. 54, 1778–1782. https://doi.org/10.1093/jme/tjx157 (2017).Article 
PubMed 

Google Scholar 
Nova Scotia Department of Natural Resources and Renewables Trees of the Acadian Forest (2021). More

Kyoto University microbiome researcher Hiroyuki Ogata says that the recent work2,3 further connects RNA viruses and the carbon pump, which affects the Earth’s biogeochemical cycles and thus its climate. And it sheds light on the diversity, evolution and ecology of RNA viruses, which has not previously been possible through applying the techniques of traditional DNA-based metagenomics. The team found many new lineages at the phylum-level by using “highly sensitive” computational approaches.It’s possible to assess the ecosystem impact of viruses by inferring auxiliary metabolic genes (AMGs). AMGs hint at the ways RNA viruses manipulate the physiology of their hosts as they seek to maximize production of more virus through the host. As Jian explains, labs have identified a variety of AMGs that are encoded by DNA viruses and, he says, it’s “well-recognized” that AMGs probably play a role in marine ecosystems. It was unknown if AMGs could be found in RNA viruses, which the recent Science paper2 has now established, he says. Jian sees this work as providing “a very important foundational dataset” for exploring questions connected to AMGs. “In my opinion, if more long-sequence or complete marine RNA virus genomes can be obtained in the future, and they can be further connected with specific hosts, it will greatly promote the understanding of the ecological impact of RNA viruses in the oceans.”To tease out AMGs, the scientists used a variety of tools, such as viral identification software for both DNA and RNA viruses, says Wainaina. The ones for DNA viruses are available on Cyverse, and the protocols for the tools from the Sullivan lab are on protocols.io. One method for RNA viruses is in progress and will be soon available on Cyverse, he says. DNA viral identification tools include VirSorter2, a pipeline for identifying viral sequence from metagenomics data, and the protocol for using this and other tools are also on protocols.io. To identify AMGs from viral sequence that had been identified through VirSorter, the team used use DRAM-v, a software tool from the lab of microbiome researcher Kelly Wrighton at Colorado State University. Her group had created Distilled and Refined Annotation of Metabolism (DRAM), a framework to resolve metabolic information from microbial data. The companion tool DRAM-v is for viruses and can be applied to metagenomic data sets for annotating metagenomics-based assembled genomes, for example through the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database, and to contiguous viral sequences identified by VirSorter.The hunt for AMGs is one instance in which the team needed to determine in each case whether a sequence was likely ‘stolen’ from host cells, says Dominguez-Huerta. RNA viral genomes are less than 40 kilobases long and usually have complicated genomic organization, both in a structural genomics sense related to the physical arrangement of genes along the viral genome and in a functional sense in terms of transcription and translation: there are overlapping genes, frameshifts and more, all of which makes this kind of annotation difficult. And sometimes information in the annotation databases is wrong and indicates that a match is cellular when it is in fact viral. Thus, to find AMGs, “we don’t have a defined clean methodology automated in a pipeline yet,” he says. It remains a time-consuming task. Assigning putative function to the protein sequences encoded by AMGs also involves checking the literature and comparing different annotation sources.Dominguez-Huerta says he and the team were glad they could assemble AMG functionalities to suggest the range of ways in which RNA viruses manipulate the metabolisms of their hosts—from photosynthesis to central carbon metabolism to vacuolar digestion and RNA repair. This overview let them see how some AMGs are repeated across different viruses across the oceans. Finding AMGs in long-read sequence is what he calls a “fire test” for the lab. To avoid ‘false AMGs’ from unreliable matches, they use BLASTP, the Basic Alignment Search Tool that compares a protein query sequence to a protein database.“I am fascinated by the ability of viruses to metabolic reprogram not only their hosts but more importantly at the ecosystem level,” says Wainaina. It is probable that the AMGs the team identified “are a central cog in microbial metabolism networks.” Current and future modeling efforts will hopefully provide insights into the ecosystem roles of viruses—both DNA viruses and RNA viruses—and on a global scale both within the ocean ecosystem and beyond.Host inference is challenging, says Dominguez-Huerta, because, for example, viruses with RNA genomes do not share genetic information with their host genomic DNA the way dsDNA viruses do when they infect bacteria. That means there is no clear signal to be derived from the host genome to help one guess the possible host. But sometimes RNA viruses do integrate into host genomes, and those, likely more accidental, events were sufficient for the scientists to capture some signal to infer hosts. “We also performed statistical co-occurrence analytics using abundances to infer the hosts with certain success,” he says.Unlike dsDNA viruses, RNA viruses infect mostly eukaryotes, from protists and fungi to invertebrates and fish larvae; only a minority infect bacteria. Overall, the team has been able to capture “a picture of dsDNA viruses infecting prokaryotes and RNA viruses infecting eukaryotes in the oceans, complementing each other in their marine hosts,” says Dominguez-Huerta. The fact that the scientists can infer “that RNA viruses can steal genes from the host,” in the form of AMGs, to then reprogram host metabolism matters not only as scientists complete the picture of how viruses directly tune the activity of hosts during infection, but also in regard to how this influences biogeochemical cycles, he says. “We think that these AMGs are incorporated into the RNA virus genomes from cellular mRNA transcripts by non-homologous recombination,” he says. This gives, in his view, a new picture of RNA viruses, which, despite their small genome sizes, can squeeze in protein-coding genes. Such proteins could be sufficient to boost the production of virus particles per infected cell, perhaps increasing viral fitness in the difficult conditions of the oligotrophic open ocean and letting the viruses better propagate in the environment.More generally, says Dominguez-Huerta, capturing RNA from ocean samples is difficult, because RNA is physically fragile and degrades rapidly. When digging into metatranscriptomic data, which include the RNA from plankton and RNA from other organisms, less than 1% of this RNA is likely to be viral RNA, he says. Previously, some labs have first purified RNA from samples, enriched it for replicating RNA viruses and then applied a method called dsRNA-seq to recover dsRNA virus sequence and replicate sequences from single-stranded RNA viruses. For future ocean RNA virus projects, he says that the lab is currently working on a wet-lab method to purify RNA virus particles from seawater to solve the challenges of obtaining viral RNA for analysis. 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Microbial biodiversity surveys have often been done in a number of generally better-studied regions3, as with the San Pedro Time Series from the San Pedro Channel off the coast of Southern California. Global surveys have also been emerging, such as the Sorcerer II Global Ocean Sampling Expedition from 2004 to 2006 launched by J. Craig Venter. There are also data and samples from the Malaspina circumnavigation, an expedition devoted to data collection on ocean biodiversity and climate change that was led by the Spanish Ministry of Science and Innovation.As microbiome researcher Shinichi Sunagawa of the ETH Zurich and colleagues point out4, sequencing technologies have advanced such that they now enable systematic and quantitative global ocean surveys. These advances, in turn, made it possible to find and assess marine double-stranded DNA virus populations. This latest work on marine RNA viruses, says Sunagawa, in which he was also involved, embeds new phylum-level findings into a “robust taxonomic framework.” In his view, this research ranks in importance with the reconstruction a few years ago of a group of bacterial genomes representing more than 35 phyla that the researchers call “the candidate phyla radiation”5. If one counts viruses in with other taxonomic groups, the finding might be the largest single expansion of established microbial taxonomy, he says. And he especially likes the definition of a new basal Orthornavirae megataxon, the proposed phylum ‘Taraviricota’. This proposed phylum is one of several findings from recently published analyses of sampling data from Tara Oceans1,2, a global expedition supported by the Tara Ocean Foundation, or Fondation Tara Océan, based in France and with many partner organizations and supporters. The foundation is a major source of global data about the ocean and ocean microbes and, as its president Étienne Bourgois says, it’s a “family project.” The family business is the French fashion house agnès b., founded by his mother Agnès Troublé.Because the family cares about the sea, they bought a 36-meter schooner from Lady Pippa Blake, widow of yachtsman and explorer Sir Peter Blake, after pirates killed him during an environmental expedition in the Amazon delta, and turned it into the expedition vessel and floating science laboratory Tara, devoted to understanding and protecting the world’s marine environment. It’s a way to continue what Peter Blake started, to continue the conversation about the ocean and do research as well, says sailor-scientist Romain Troublé, executive director of the foundation and nephew of Agnès Troublé. The boat had been previously owned by explorer Jean-Louis Étienne. The foundation has supported several expeditions with Tara including the Tara Oceans and Tara Oceans Polar Circle expeditions, as well as Tara Mission Microbiomes, which is currently underway. The equilibrium of the planet “depends on the microbiome of the ocean in the same way we depend on our own microbiome,” says Romain Troublé. Viruses are part of the larger picture of how life is supported on the planet. It’s “a great mystery of the century” to decipher the roles, behaviors and functions of the ocean microbiome, including its beneficial effects. Over the last decade, he says, the expeditions have, for example, collected plankton samples from coastal waters, coral reefs and the high seas around the world for scientists to ask questions of. Microplastics in the ocean concentrate chemical pollutants such as pesticides, and microplastics appear to be substrates for distinct microbiomes. Polystyrene and polypropylene, for example, harbor different microbial communities. “We call it the plastisphere,” he says. All sample collection, not just of microplastics, happens with a view to scientific rigor to assure data quality, says Troublé. Many institutes are part of and support the expeditions through the Tara Ocean Foundation, including AtlantECO, the French Ministry of Research, the Swiss National Science Foundation, the US National Science Foundation, the European Molecular Biology Laboratory and the French National Centre for Scientific Research.Tara Oceans was an expedition initiated by EMBL researcher Eric Karsenti, here in the foreground. He is checking a rosette of Niskin bottles that collect water, and ocean microbe samples, at various depths. Sensors capture parameters such as temperature.
Credit: Fondation Tara OcéanIts expedition Tara Oceans was initiated by cell and marine biologist Eric Karsenti of the European Molecular Biology Laboratory. The expedition ran from 2009 to 2013 and covered 125,000 kilometers of ocean, taking ocean water and samples. It collected nearly 35,000 samples of viruses, algae and plankton and delivered more than 60 terabases of DNA and RNA sequences.The research community strives to follow FAIR data principles, the principles of findability, accessibility, interoperability and reusability, says Sunagawa. Tara Ocean’s data troves can be found, for instance, in the European Nucleotide Archive (ENA), Pangeaea, Cyverse, iVIRUS and on Genoscope. Other data-collection efforts target users with less programming experience and offer various types of data relevant to marine microbial research, he says: for example, the Ocean Gene Atlas, a portal to search for a gene or protein sequence to see, for instance, its abundance on an ocean map. The Ocean Barcode Atlas lets users explore, for example, operational taxonomic units (OTU) data and plankton communities from Tara Oceans and OTUs from Malaspina prokaryote data. Sunagawa also points to the Ocean Microbiomics Database and its high-quality genome-resolved information about the global microbiome, which has sequencing data from 2003 onwards and which includes Tara Oceans data as well as datasets such as the Hawaii Ocean Time-Series (HOT), the Bermuda Atlantic Time-series Study (BATS), with its collection of ocean data dating back to 1988, and BioGeotraces, with hydrographic and marine geochemical data from various expeditions.The recent publications on RNA viruses1,2, in which Sunagawa was also involved, have expanded the known diversity of these viruses, he says. They build on efforts by, for example, the research team that created and applied a cloud-based infrastructure called Serratus6, with which researchers can perform sequence alignment using bowtie2 for nucleotide sequences and DIAMOND2 for protein sequences in ‘ultra-high throughput’ on a petabase scale. Using Serratus, the team identified more than 130,000 previously unknown RNA viruses, both on land and in the oceans. The wealth of resources for microbial and viral data about the oceans is helpful to the research community, but “we could still improve the connectivity between various datasets though,” says Sunagawa. That would help, for example, with searching and finding data products that are derived from primary data, such as identifiers of individual genome assemblies, genes and metagenome assembled genomes, which are all presented in different online locations. But connecting data resources is a project that itself takes resources, and such projects are hard to get funding for.Going forward, it will be challenging, says Sunagawa, to update and keep up to date both past projects and ongoing projects such as the Global Ocean Ship-based Hydrographic Investigations program (GO-SHIP), which is focused on physical oceanography; the Antarctic Circumnavigation Expedition (ACE), on carbon-cycle marine biogeochemistry; Mission Microbiomes; and many more. “And ultimately, we will need to cross boundaries that currently separate biome-focused research to better understand processes at the sea–land–atmosphere interfaces.”Tara Mission Microbiomes has been underway for nearly two years and wraps up in October 2022. At press time, the schooner Tara was off the Angolan Coast. At the end of the expedition, it will have traveled a total of 70,000 km of ocean area around South America, Africa, Europe and Antarctica. Mission Microbiomes is part of the EU-funded AtlantECO and also includes 42 research organizations from 13 countries. The microbiome mission is collecting data on how climate change is affecting the marine microbiome, on how pollution, microplastics pollution in particular, affects the marine environments and on the beneficial impact of the ocean microbiome.Krill are small ocean crustaceans that mainly eat phytoplankton and are a food source for animals such as whales and seals. Krill play a crucial role in biogeochemical cycles.
Credit: F. Aurat, Fondation Tara OcéanChris Bowler, from the Institut de Biologie de l’École Normale Supérieure, is scientific director of the Tara Oceans consortium, was scientific coordinator of the Tara Oceans expedition and was onboard in Antarctica during the Tara Mission Microbiomes expedition to collect data on the impact of icebergs on the Weddell Sea ecosystem. The project’s scientists in Tara Mission Microbiomes, he says, are studying specific processes, including the Amazon plume, the Malvinas confluence, the impact of tabular icebergs in the Weddell Sea, the Benguela upwelling and more. The data from this expedition will be similar to those from Tara Oceans but, he says, “we will have much more contextual data related to the specific processes we have been studying.” The applied techniques are all ones that have undergone much advancement since Tara Oceans, he says. They include long-read sequencing, Hi-C sequencing to capture chromatin organization on a genome-wide basis and various types of microscopy.Data and results from previous and ongoing expeditions are impressive, says Sunagawa but “we are still data-limited in our field of research.” Geographically, sampling stations are usually still separated by hundreds of kilometers, and often they are even further apart than that. This means that what is missing is both temporal and seasonal resolution, “and we keep detecting new organisms,” he says. Tara Mission Microbiomes will help to fill in some of these gaps. The mission is unlike Tara Oceans, with its focus more on coastal areas and environmental pollutants such as microplastics. Sunagawa and his group are not currently involved with Tara Mission Microbiomes, “but we look forward to seeing the first results coming out soon.”Through photosynthesis, phytoplankton deliver oxygen to the planet. They are food for zooplankton, which are food for other marine organisms. This food web and its associated decomposition are part of the ocean’s carbon pump, in which marine viruses play an important role that scientists have only begun exploring.
Credit: M. Bardy, Fondation Tara Océan More

Response of chlorophytes to environmental variables in field vs. forest pondsOur study demonstrated that human-originated transformation in the catchment area surrounding a small water body may influence the water conditions in terms of physical, chemical, and biological parameters as well as the ecological state of the aquatic environment in respect to green algae communities.Chlorophytes inhabiting field ponds were more abundant compared with the forest ponds. This shows that field ponds, due to the higher values of TRP and water conductivity, created favorable conditions for chlorophyte development. The high concentrations of TRP and conductivity in aquatic environments are characteristic in the case of agricultural catchments exposed to anthropogenic pressure because of the inflow from the surrounding fertilized fields42. In this type of pond, we also observed significantly higher water temperatures and pH due to the lack of trees around them compared to the forest ponds, two factors which also positively influenced the growth of chlorophytes. Both the higher light intensity and the smaller size of the field ponds cause earlier warming up than the forest ponds and give an advantage to high light tolerant species. Moreover, it is well known that an increase in temperature stimulates the release of phosphorus from the bottom sediments, so this could be another reason for the higher levels of TRP in the field ponds. Our CCA analysis showed that TRP and conductivity were the strongest determinants of the distribution of chlorophyte species in the examined water bodies. We found a large group of dominant species indicated high values of TRP (e.g. Ankistrodesmus falcatus, A. arcuatus, Monoraphidium griffithii, Pseudopediastrum boryanum, Pediastrum duplex, Scenedesmus obtusus, Scenedesmus arcuatus var. gracilis, Desmodesmus communis, Coelastrum microporum), and another group of species (e.g. Kirchneriella irregularis var. spiralis, Tetraedron minimum, Scenedesmus ecornis) that preferred high levels of conductivity.In the field ponds generally higher mean abundances of filtrators and Rotifera were observed. This could be another important factor stimulating the growth of chlorophytes and increasing their abundances by the resupply of nutrients through excretion43,44. On the other hand, the high densities of algae could be the factor that caused better zooplankton development, and therefore its abundance in field ponds was greater. Filtrating cladocerans and Rotifera also had a significant influence on the distribution of chlorophyte dominating species. However, even though the total abundance of both chlorophytes and filtering zooplankton was greater in the field ponds, CCA analysis revealed a negative relationship existing between filtrators and most dominant species of chlorophytes (e.g. Pandorina morum, Willea rectangularis, Desmodesmus armatus, Nephrochlamys willeana, Cosmarium trilobulatum). Only two chlorophyte species—Lemmermannnia tetrapedia and Tetraedron triangulare—co-occurred with cladoceran zooplankton. These latter species are very small compared to the species above and can therefore be overlooked by filtrators, which have a choice of larger and perhaps more nutritiously satisfying algae of the genus Pandorina, Crucigeniella, Cosmarium or Nephrochlamys, but still of a size suitable for zooplankton. It can also be interpreted in such a way that Crucigenia and Tetraedron are among the r-strategists that reproduce very quickly, so grazing pressure by zooplankton can stimulate their rapid development45 and thus they remain at a stable level.Specific environmental conditions prevailing in the field ponds resulted in a high number of exclusive taxa44, found only in this type of water body. Moreover, a greater diversity of the representatives of different functional groups were found here, compared to the forest ponds.Analyzing the distribution of chlorophytes in terms of phytoplankton functional groups39,40, we found that group W1 was represented by only one species, Gonium pectorale. This was especially noted in the field water bodies. This group is known to prefer small water bodies rich in organic matter from husbandry or sewage40, which suggests that the field catchment in our study migh be a supplier of these substances. It also proves that field surroundings are far more human impacted. In the field ponds we observed a higher abundance of chlorophytes belonging to the groups G (Eudorina elegans, Pandorina morum, Pandorina smithii and Volvox aureus), J (e.g. representatives of the genus Actinastrum, Chlorotetraedron, Coelastrum, Crucigenia, Desmodesmus/Scenedesmus, Golenkinia, Pediastrum, Tetraedron, Tetrastrum, Westella, Willea/Crucigeniella), W0 (genera Chlamydomonas, Chlorangiopsis, Chlamydomonadopsis, Planktococcomyxa/Coccomyxa) and X3 (Chlorella sp.), typical for shallow nutrient-rich waters (G and J), ponds with extremely high organic contents (W0), and for shallow well-mixed layers (X3), according to classification given by Padisak et al.40. Considering that nitrogen compounds had a similar level in both types of ponds it can be stated that the representatives of the above mentioned functional groups of chlorophytes associated with the field ponds were presumably dependent on higher concentrations of TRP and conductivity and not that much on nitrogen concentrations.In the forest ponds significantly higher values of water saturation were recorded compared to the field ponds. Moreover, the lack of inflow of fertilizers from the catchment area resulted in lower TRP concentrations, which along with lower water temperatures, pH and conductivity in the forest ponds may have contributed to the reduced abundance of chlorophytes compared to the field water bodies. RDA analysis showed that some dominant chlorophyte species (e.g. Closterium moniliferum, Closterium tumidulum, Cosmarium trilobulatum and Mougeotia sp.) were associated with this type of small water body. At the same time the abundance of these species was smaller in the field ponds. We also found that chlorophyte diversity (Shannon–Weaver index) was greater in the forest ponds. This suggests that water bodies located within the forested area, usually more natural ponds being less exposed to anthropogenic pressure, are characterized by greater biodiversity. Moreover, in this type of water body we found many exclusive species39, not reported from the field ponds. Interestingly, about the half of these taxa belonged to desmids, which prefer lower pH and conductivity46, conditions typical for forest ponds. This could be also a reason for the dominance of desmid species with the highest abundance/frequency, associated with forest ponds.Taking into consideration the phytoplankton functional groups39,40 our study showed that the chlorophytes associated with forest ponds prefer mesotrophic waters (from the group TD: Cladophora glomerata, Geminella turfosa, Geminella planctonica, Microspora sp., Netrium digitus, Oedogonium sp., Oocystidium ovale, Spirogyra sp. Zygnema sp. and those belonging to the group N: mainly genera Closterium, Cosmarium, Euastrum, Micrasterias, Staurastrum, Staurodesmus, Xanthidium). This explains their greater share in the less fertile forest ponds. Another group associated with the forest ponds – T (Mougeotia sp., Binuclearia lauterbornii) contains species tolerant to light deficiency, so they were able to develop well in the more shaded water bodies located in the forest catchment.Chlorophyte community structure in two types of habitats (open water vs. macrophyte-dominated zone)In our study, the type of habitat (open water and macrophyte-dominated zones) also had a significant structuring effect on chlorophytes. There were a group of species linked to the open water zone (Pandorina morum, Nephrochlamys willeana, Oocystis lacustris, Scenedesmus armatus, Scenedesmus intermedius and Desmodesmus communis), being negatively related to vegetated stations at the same time. Generally, we found here a higher mean abundance of chlorophytes compared to the macrophyte-dominated zones, possibly due to the higher values of nutrients such as NH4 and TRP, the conditions favouring the development of many algae species. The results of the CCA analysis with habitats confirmed the high importance of both nutritional factors in structuring the distribution of chlorophyte species. There was a group of species associated with a rise in the concentration of ammonium (e.g. Scenedesmus arcuatus var. gracilis, Pediastrum duplex, Closterium moniliferum, Closterium tumidulum, Cosmarium trilobulatum, Willea rectangularis) as well as with phosphates (Monoraphidium tortile, Scenedesmus ecornis, Tetradesmus lagerheimii and Tetraedron minimum). Generally, high abundance of chlorophytes in the open water area was accompanied by a small-sized fraction of zooplankton–rotifers. Therefore, rotifers had a lower impact on the distribution of chlorophytes than filtrators. The increasing numbers of cladocerans contributed to the lowering abundance of some chlorophytes, such as Monoraphidium tortile, Scenedesmus ecornis, Tetradesmus lagerheimii or Tetraedron minimum. This shows that filtrators, whose densities were significantly higher among macrophytes, were able to control the development of some chlorophyte species much more efficiently than small-bodied rotifers.The effect of habitat was also visible in the case of phytoplankton functional groups39,40. We found that representatives of the group N (e.g. Closterium, Cosmarium, Euastrum, Micrasterias, Staurastrum) had a significantly higher mean abundance in the open water zones compared to the macrophyte-dominated zones. Interestingly, according to Padisak et al.40 group N prefers less fertile (mesotrophic) conditions, which is inconsistent with our results. However, we think that their association with the open water sites could be connected rather with the place/level where they live in the water column, rather than with the trophic state of water. The above mentioned chlorophytes taxonomically belong to desmids, which are mostly benthic organisms. Their greater quantitative share in the samples from the open water areas could be an effect of the intensive water mixing in the shallow ponds due to the lack of macrophytes. Neustupa et al.47 confirm that desmids are able to form tychoplanktonic communities due to water movements. In the samples collected from the macrophyte-dominated stations the mean abundance of desmids was generally lower, probably because of the macrophyte stabilizing effect. Aquatic plants are known to reduce turbidity and stabilize bottom sediments48, so they can prevent any intensive water mixing in ponds. In the examined open water stations, we also found a higher mean abundance of chlorophytes typical for shallow nutrient-rich waters (group G: Eudorina, Pandorina, Volvox and group K: Radiococcus) and/or for ponds with extremely high organic contents (group W0: e.g. Chlamydomonas), which proves that the sites lacking macrophytes were more fertile. Additionally, clearly more representatives from the codon J and X1 (typical for waters with high trophic levels) and a greater diversity of the representatives of different functional groups were recorded in the open water area compared to the macrophyte-dominated zones.The macrophyte-dominated stations had more abundant communities of filtrators, as aquatic plants are known to provide a profitable shelter for zooplankton49. Cladoceran predominance among macrophytes may have been a force reducing green algae numbers. The chlorophytes of the investigated ponds were mostly small- or medium-size species. Their size distribution makes them a high quality food for zooplankton, particularly for cladoceran filtrators. According to RDA analysis apart from pond size, the presence of filtrators significanly reduced the abundance of several chlorophyte dominating species. The lower algae abundance among macrophytes compared to the open water zone could also be explained by competition between algae and macrophytes for light and nutrients37,50 and/or with the secretion of allelopathic substances e.g. by Ceratophyllum demersum51 inhibiting algal development. Our studies demonstrated that among chemical factors which clearly differentiated the two types of analysed habitat, TRP and NH4 significantly influenced the distribution of chlorophyte dominating species. The lower levels of these parameters in macrophyte-dominated zones suggest that the nutrient uptake by aquatic plants in the investigated water bodies was high. There are many reports on the decrease of nutrient concentrations by macrophytes30,37,52, which are consistent with our observations. Despite lower, compared to the open water zone, chlorophyte densities within the macrophyte-dominated zones there was a group of species (e.g. Mougeotia sp., Pediastrum tetras, Scenedesmus obtusus, Monoraphidium contortum) that selectively chose vegetated stands. Furthermore, we found a great number29 of exclusive chlorophyte species for macrophyte-dominated zones. Half of these taxa belong to desmids, which are often periphytic organisms associated with aquatic macrophytes53,54.Preference towards macrophyte-dominated stations was also documented for two phytoplankton functional groups (T: Mougeotia sp. and Binuclearia lauterbornii and TD: e.g., Spirogyra sp., Zygnema sp., Cladophora glomerata, Oedogonium sp.) and one group which occurred exlusively among vegetated sites (MP—Ulothrix). Interestingly, all the representatives of these groups had a similar filamentous morphological form, which suggests that many of them are of epithytic origin, coexisting within aquatic plants. Two more groups—X2 (Pseudodidymocystis/Didymocystis, Pteromonas) and W1 (Gonium pectorale) were clearly affected by the presence of macrophytes. According to Padisak et al.40, codons TD and X2 indicate mesoeutrophic conditions and their higher abundances in the macrophyte-dominated zones also proves that plants contribute to lowering the trophic levels in the examined ponds. On the other hand, the relatively high abundance of the representative of the group W1 in these habitats suggests that macrophytes could enrich ponds with organic matter during the process of their decomposition.Concluding, our results prove that different types of catchment area (field and forest) as well as different types of habitats (open water zone and macrophyte-dominated zone) create distinct, specific conditions (dependent on some physical–chemical and biological variables) for the occurrence of chlorophytes in small water bodies. We conclude that cosmopolitan chlorophytes undoubtedly respond to the level of habitat heterogeneity, contributing to the ecological assessment of small water bodies. Chlorophytes in particularl react to the level of human transformation in the ponds’ vicinities. This is why we suggest using them for water quality evaluation in ponds. This interdisciplinary research significantly broadens the knowledge, not only about the response of chlorophytes to physical–chemical parameters of water, but also about the food preferences of zooplankton for which green algae are the basic food, and vice versa about the impact of zooplankton on microalgae communities. The analyses provide valuable information on chlorophytes-zooplankton interactions and also about the relationships between chlorophytes and macrophytes. Received data emphasize the high value of field ponds, underestimated habitats particularly vulnerable to destruction in the agricultural landscape. The research will help to better understand the functioning of poorly studied small water bodies, which will contribute to the preservation of their biodiversity and protection against degradation. They will also be useful in the management of small water bodies based on the specificity of chlorophyte occurrence in various habitats and catchment type ponds. Moreover, these results are important in a broader context, as the interactions between the studied organisms and the physico-chemical parameters of water in small bodies of water are to some extent universal, so the analyses will broaden the knowledge about the functioning of larger bodies of water. More

Benelli, G. et al. Sexual communication and related behaviours in Tephritidae: Current knowledge and potential applications for Integrated Pest Management. J. Pest Sci. 87, 385–405 (2014).Article 

Google Scholar 
Kuba, H. & Sokei, Y. The production of pheromone clouds by spraying in the melon fly, Dacus cucurbitae coquillett (Diptera: Tephritidae). J. Ethol. 6, 105–110 (1988).Article 

Google Scholar 
Fletcher, B. S. The structure and function of the sex pheromone glands of the male Queensland fruit fly, Dacus tryoni.. J. Insect Physiol. 15, 1309–1322 (1969).Article 

Google Scholar 
Nation, J. L. Courtship behavior and evidence for a sex attractant in the male Caribbean fruit fly, Anastrepha suspensa. Ann. Entomol. Soc. Am. 65, 1364–1367 (1972).Article 

Google Scholar 
Arita, L. H. & Kaneshiro, K. Y. Sexual selection and lek behavior in the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae). Pacific Sci. (EUA) 43, 135–143 (1989).
Google Scholar 
Briceño, R.D. & Eberhard, W.G. Male wing positions during courtship by Mediterranean fruit flies (Ceratitis capitata) (Diptera: Tephritidae). J. Kansas Entomol. Soc. 143–47 (2000).Benelli, G. et al. Male wing vibration in the mating behavior of the Olive fruit fly Bactrocera oleae (Rossi) (Diptera: Tephritidae). J. Insect Behav. 25, 590–603 (2012).Article 

Google Scholar 
Feron, M. L’appel sonore du mâle dans le comportement sexuel de Dacus oleae Gmel [Dipt Trypetidae]. Bull. Soc. Entomol. Fr. 65, 139–143 (1960).Article 

Google Scholar 
Feron, M. & Andrieu, A. J. Etude des signaux acoustiques du male dans le comportement sexuel de Dacus Oleae Gmel (Dipt. Trypetidae). Ann. Epiphyt. 13, 269–276 (1962).
Google Scholar 
Rolli, K. Die akustischen Sexualsignale von Ceratitis capitata Wied. Und Dacus oleae Gmel. Z. Angew. Entomol. 81, 219–223 (1976).Article 

Google Scholar 
Webb, J. C., Calkins, C. O., Chambers, D. L., Schwienbacher, W. & Russ, K. Acoustical aspects of behavior of Mediterranean fruit fly, Ceratitis capitata: Analysis and identification of courtship sounds. Entomol. Exp. Appl. 33, 1–8 (1983).Article 

Google Scholar 
Mankin, R. W., Lemon, M., Harmer, A. M. T., Evans, C. S. & Taylor, P. W. Time pattern and frequency analyses of sounds produced by irradiated and untreated male Bactrocera tryoni (Diptera: Tephritidae) during mating behavior. Ann. Entomol. Soc. Am. 101, 664–674 (2008).Article 

Google Scholar 
Miyatake, T. & Kanmiya, K. Male courtship song in circadian rhythm mutants of Bactrocera cucurbitae (Tephritidae: Diptera). J. Insect Physiol. 50, 85–91 (2004).CAS 
PubMed 
Article 

Google Scholar 
Sivinski, J., Burk, T. & Webb, J. Acoustic courtship signals in the Caribbean fruit fly, Anastrepha suspensa (Loew). Anim. Behav. 32, 1011–1016 (1984).Article 

Google Scholar 
Mankin, R. W. et al. Broadcasts of wing-fanning vibrations recorded from calling male Ceratitis capitata (Diptera: Tephritidae) increase captures of females in traps. J. Econ. Entomol. 97, 1299–1309 (2004).CAS 
PubMed 
Article 

Google Scholar 
Mankin, R. W., Petersson, E., Epsky, N. D., Heath, R. R. & Sivinski, J. Exposure to male pheromones enhances Anastrepha suspensa (Diptera: Tephritidae) female response to male calling song. Fla. Entomol. 83, 411 (2000).CAS 
Article 

Google Scholar 
Sivinski, J. & Webb, J. C. Changes in a Caribbean fruit fly acoustic signal with social situation (Diptera: Tephritidae)1. Ann. Entomol. Soc. Am. 79, 146–149 (1986).Article 

Google Scholar 
Canale, A. et al. The courtship song of fanning males in the fruit fly parasitoid Psyttalia concolor (Szépligeti) (Hymenoptera: Braconidae). Bull. Entomol. Res. 103, 303–309 (2013).CAS 
PubMed 
Article 

Google Scholar 
Wicker-Thomas, C. Pheromonal communication involved in courtship behavior in Diptera. J. Insect. Physiol. 53, 1089–1100 (2007).CAS 
PubMed 
Article 

Google Scholar 
Tan, K.H., Nishida, R., Jang, E.B. & Shelly, T.E. Pheromones, male lures, and trapping of tephritid fruit flies. In: Trapping and the Detection, Control, and Regulation of Tephritid Fruit Flies: Lures, Area-Wide Programs, And Trade Implications 15–74 (Springer, 2014).Poramarcom, R. Sexual communication in the Oriental fruit fly, Dacus dorsalis Hendel (Diptera: Tephritidae). Doctoral dissertation. (University of Hawaii at Manoa, 1988).Ekanayake, D. The mating system and courtship behaviour of the Queensland fruit fly, Bactrocera tryoni (Froggatt) (Diptera: Tephritidae). Doctoral dissertation. (Queensland University of Technology, 2017).Suzuki, Y. & Koyama, J. Courtship behavior of the melon fly, Dacus cucurbitae Coquillett (Diptera: Tephritidae). Appl. Entomol. Zool. 16, 164–166 (1981).Article 

Google Scholar 
Scolari, F., Valerio, F., Benelli, G., Papadopoulos, N. T. & Vaníčková, L. Tephritid fruit fly semiochemicals: Current knowledge and future perspectives. Insects 12, 408 (2021).PubMed 
PubMed Central 
Article 

Google Scholar 
Nieri, R., Anfora, G., Mazzoni, V. & Rossi Stacconi, M. V. Semiochemicals, semiophysicals and their integration for the development of innovative multi-modal systems for agricultural pests’ monitoring and control. Entomol. Gen. 42, 167–183 (2022).Article 

Google Scholar 
Cocroft, R. B. & Rodríguez, R. L. The behavioral ecology of insect vibrational communication. Bioscience 55, 323–334 (2005).Article 

Google Scholar 
Daane, K. M. & Johnson, M. W. Olive fruit fly: Managing an ancient pest in modern times. Annu. Rev. Entomol. 55, 151–169 (2010).CAS 
PubMed 
Article 

Google Scholar 
Rice, R. E., Phillips, P. A., Stewart-Leslie, J. & Sibbett, G. S. Olive fruit fly populations measured in Central and Southern California. Calif. Agric. 57, 122–127 (2003).Article 

Google Scholar 
Wang, X. et al. Exploration for olive fruit fly parasitoids across Africa reveals regional distributions and dominance of closely associated parasitoids. Sci. Rep. 11, 1–14 (2021).Article 
CAS 

Google Scholar 
Loher, W. & Zervas, G. The mating rhythm of the olive fruitfly, Dacus oleae Gmelin. Z. Angew. Entomol. 88, 425–435 (1979).Article 

Google Scholar 
Benelli, G. Aggression in Tephritidae flies: Where, when, why? Future directions for research in integrated pest management. Insects 6, 38–53 (2014).PubMed 
PubMed Central 
Article 

Google Scholar 
Benelli, G. Aggressive behavior and territoriality in the olive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae): Role of residence and time of day. J. Insect. Behav. 27, 145–161 (2014).Article 

Google Scholar 
Shelly, T. E. Aggression between wild and laboratory-reared sterile males of the mediterranean fruit fly in a natural habitat (Diptera: Tephritidae). Fla. Entomol. 83, 105–108 (2000).Article 

Google Scholar 
Ekanayake, W. M., Clarke, A. R. & Schutze, M. K. Close-distance courtship of laboratory reared Bactrocera tryoni (Diptera: Tephritidae). Austral. Entomol. 58, 578–588 (2019).Article 

Google Scholar 
Ant, T. et al. Control of the olive fruit fly using genetics-enhanced sterile insect technique. BMC Biol. 10, 1–8 (2012).Article 

Google Scholar 
Estes, A. M. et al. A basis for the renewal of sterile insect technique for the olive fly, Bactrocera oleae (Rossi). J. Appl. Entomol. 136, 1–16 (2012).Article 

Google Scholar 
Zanini, D., Geurten, B., Spalthoff, C. & Göpfert, M. C. Sound communication in Drosophila. In Insect Hearing and Acoustic Communication Animal Signals and Communication, Vol. 1 (ed. Hedwig, B.) (Springer, 2014).
Google Scholar 
Windmill, J. F. C. & Jackson, J. C. Mechanical specializations of insect ears. In Insect Hearing. Springer Handbook of Auditory Research, Vol. 55 (eds Pollack, G. et al.) (Springer, 2016).
Google Scholar 
Talyn, B. C. & Dowse, H. B. The role of courtship song in sexual selection and species recognition by female Drosophila melanogaster. Anim. Behav. 68, 1165–1180 (2004).Article 

Google Scholar 
Kanmiya, K. Acoustic studies on the mechanism of sound production in the mating songs of the melon fly, Dacus cucurbitae Coquillett (Diptera: Tephritidae). J. Ethol. 6, 143–151 (1988).Article 

Google Scholar 
Benelli, G. et al. Wing-fanning frequency as a releaser boosting male mating success—High-speed video analysis of courtship behavior in Campoplex capitator, a parasitoid of Lobesia botrana. Insect Sci. 27, 1298–1310 (2020).PubMed 
Article 

Google Scholar 
Ge, J. et al. Pea leafminer Liriomyza huidobrensis (Diptera: Agromyzidae) uses vibrational duets for efficient sexual communication. Insect Sci. 26, 510–522 (2019).PubMed 
Article 

Google Scholar 
Mazzoni, V., Anfora, G. & Virant-Doberlet, M. Substrate vibrations during courtship in three drosophila species. PLoS ONE 8, e80708 (2013).ADS 
PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
McKelvey, E. G. Z. et al. Drosophila females receive male substrate-borne signals through specific leg neurons during courtship. Curr. Biol. 31, 3894–3904 (2021).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Strauß, J., Stritih-Peljhan, N., Nieri, R., Virant-Doberlet, M., & Mazzoni, V. Communication by substrate-borne mechanical waves in insects: From basic to applied biotremology. In: Advances in Insect Physiology, vol. 61, 189–307 (Academic Press, 2021).Mazomenos, B. E. Effect of age and mating on pheromone production in the female olive fruit fly, Dacus oleae (Gmel.). J. Insect Physiol. 30, 765–769 (1984).CAS 
Article 

Google Scholar 
Carpita, A. et al. (Z)-9-tricosene identified in rectal gland extracts of Bactrocera oleae males: First evidence of a male-produced female attractant in in olive fruit fly. Naturwissenschaften 99, 77–81 (2012).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Canale, A. et al. Behavioural and electrophysiological responses of the olive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae), to male- and female-borne sex attractants. Chemoecology 23, 155–164 (2013).CAS 
Article 

Google Scholar 
Mcdonald, P. T. Intragroup stimulation of pheromone release by male mediterranean fruit flies (Diptera: Tephritidae). Ann. Entomol. Soc. Am. 80, 17–20 (1987).CAS 
Article 

Google Scholar 
Iwahashi, O. & Majima, T. Lek formation and male–male competition in the melon fly, Dacus cucurbitae Coquillett: Diptera: Tephritidae. Appl. Entomol. Zool. 21, 70–75 (1986).Article 

Google Scholar 
Keiser, I., Kobayashi, R. M., Chambers, D. L. & Schneider, E. L. Relation of sexual dimorphism in the wings, potential stridulation, and illumination to mating of oriental fruit flies, melon flies, and Mediterranean fruit flies in Hawaii. Ann. Ent. Soc. Am. 66, 937–941 (1973).Article 

Google Scholar 
Benelli, G. & Canale, A. Aggressive behavior in olive fruit fly females: Oviposition site guarding against parasitic wasps. J. Insect Behav. 29, 680–688 (2016).Article 

Google Scholar 
Rohde, B. B. et al. An acoustic trap to survey and capture two neoscapteriscus species. Fla. Entomol. 102, 654–657 (2019).Article 

Google Scholar 
Shelly, T. E. Lek size and female visitation in two species of tephritid fruit flies. Anim. Behav. 62, 33–40 (2001).Article 

Google Scholar 
Niyazi, N., Shuker, D. M. & Wood, R. J. Male position and calling effort together influence male attractiveness in leks of the medfly, Ceratitis capitata (Diptera: Tephritidae): Male attractiveness in leks of Ceratitis capitata. Biol. J. Linn. Soc. Lond. 95, 479–487 (2008).Article 

Google Scholar 
Greenfield, M. D. Signal interactions and interference in insect choruses: Singing and listening in the social environment. J. Comp. Physiol. A 201, 143–154 (2015).Article 

Google Scholar 
Kouloussis, N. A. et al. Age related assessment of sugar and protein intake of Ceratitis capitata in ad libitum conditions and modeling its relation to reproduction. Front. Physiol. 8, 1–13 (2017).Article 

Google Scholar 
Boersma, P. & Van Heuven, V. Speak and unSpeak with PRAAT. Glot Int. 5, 341–347 (2001).
Google Scholar 
Joyce, A. L. et al. Effect of continuous rearing on courtship acoustics of five braconid parasitoids, candidates for augmentative biological control of Anastrepha species. Biocontrol 55, 573–582 (2010).Article 

Google Scholar 
Sall, J. et al. JMP Start Statistics: A Guide to Statistics and Data Analysis Using JMP (Sas Institute, 2017).
Google Scholar  More

Pienta, K. J., Hammarlund, E. U., Austin, R. H., Axelrod, R., Brown, J. S. & Amend, S. R. Cancer cells employ an evolutionarily conserved polyploidization program to resist therapy. In Seminars in Cancer Biology, 1–15 (2020).Siegel, R. L., Miller, K. D. & Jemal, A. Cancer statistics, 2020. CA A Cancer J. Clin. 70(1), 7–30 (2020).Article 

Google Scholar 
Duesberg, P. & Rasnick, D. Aneuploidy, the somatic mutation that makes cancer a species of its own. Cell Motil. Cytoskelet. 47(2), 81–107 (2000).CAS 
Article 

Google Scholar 
Hanahan, D. & Weinberg, R. A. Leading edge review hallmarks of cancer: The next generation. Cell 144, 646–674 (2011).CAS 
PubMed 
Article 

Google Scholar 
Amend, S. R. et al. Polyploid giant cancer cells: Unrecognized actuators of tumorigenesis, metastasis, and resistance. Prostate 79(13), 1489–1497 (2019).PubMed 
PubMed Central 
Article 

Google Scholar 
Pienta, K. J. et al. Convergent evolution, evolving evolvability, and the origins of lethal cancer. Mol. Cancer Res. 18(6), 801–810 (2020).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Pienta, K. J., Hammarlund, E. U., Axelrod, R., Brown, J. S. & Amend, S. R. Poly-aneuploid cancer cells promote evolvability, generating lethal cancer. Evol. Appl. 13(7), 1626–1634 (2020).PubMed 
PubMed Central 
Article 

Google Scholar 
Roychowdhury, S. et al. Personalized oncology through integrative high-throughput sequencing: A pilot study. Sci. Transl. Med. 3(111), 1–12 (2011).Article 
CAS 

Google Scholar 
Kuczler, M. D., Olseen, A. M., Pienta, K. J. & Amend, S. R. ROS-induced cell cycle arrest as a mechanism of resistance in polyaneuploid cancer cells (PACCs). Prog. Biophys. Mol. Biol. 165, 3–7 (2021).CAS 
PubMed 
Article 

Google Scholar 

Brown, R. L. What evolvability really is. Br. J. Philos. Sci.65(3), 549–572 (2014).MathSciNet 
Article 

Google Scholar 
Crother, B. I. & Murray, C. M. Early usage and meaning of evolvability. Ecol. Evol. 9(7), 3784–3793 (2019).PubMed 
PubMed Central 
Article 

Google Scholar 
Payne, J. L. & Wagner, A. The causes of evolvability and their evolution. Nat. Rev. Genet. 20, 24–38 (2019).CAS 
PubMed 
Article 

Google Scholar 
Pigliucci, M. Is evolvability evolvable?. Genetics 9, 75–82 (2008).CAS 
PubMed 

Google Scholar 
Sniegowski, P. D. & Murphy, H. A. Evolvability. Curr. Biol. 16, R831–R834 (2006).CAS 
PubMed 
Article 

Google Scholar 
Kostecka, L. G., Pienta, K. J. & Amend, S. R. Polyaneuploid cancer cell dormancy: Lessons from evolutionary phyla. Front. Ecol. Evol. 9, 439 (2021).Article 

Google Scholar 
Rajaraman, R., Rajaraman, M. M., Rajaraman, S. R. & Guernsey, D. L. Neosis–a paradigm of self-renewal in cancer. Cell Biol. Int. 29(12), 1084–1097 (2005).CAS 
PubMed 
Article 

Google Scholar 
Rajaraman, R., Guernsey, D. L., Rajaraman, M. M. & Rajaraman, S. R. Neosis–A parasexual somatic reduction division in cancer. Int. J. Hum. Genet. 7(1), 29–48 (2007).CAS 
Article 

Google Scholar 
Sundaram, M., Guernsey, D. L., Rajaraman, M. M. & Rajaraman, R. Neosis: A novel type of cell division in cancer. Cancer Biol. Ther. 3(2), 207–218 (2004).CAS 
PubMed 
Article 

Google Scholar 
Gatenby, R. A., Cunningham, J. J. & Brown, J. S. Evolutionary triage governs fitness in driver and passenger mutations and suggests targeting never mutations. Nat. Commun. 5(1), 1–9 (2014).Article 

Google Scholar 
Bukkuri, A. & Brown, J. S. Evolutionary game theory: Darwinian dynamics and the G function approach. MDPI Games 12(4), 1–19 (2021).MathSciNet 
MATH 

Google Scholar 
Lopez-Sánchez, L. M. et al. CoCl2, a mimic of hypoxia, induces formation of polyploid giant cells with stem characteristics in colon cancer. PLoS ONE 9(6), e99143 (2014).ADS 
PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
Mittal, K. et al. Multinucleated polyploidy drives resistance to Docetaxel chemotherapy in prostate cancer. Br. J. Cancer 116(9), 1186–1194 (2017).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Niu, N., Mercado-Uribe, I. & Liu, J. Dedifferentiation into blastomere-like cancer stem cells via formation of polyploid giant cancer cells. Oncogene 36(34), 4887–4900 (2017).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Ogden, A., Rida, P. C. G., Knudsen, B. S., Kucuk, O. & Aneja, R. Docetaxel-induced polyploidization may underlie chemoresistance and disease relapse. Cancer Lett. 367, 89–92 (2015).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Puig, P. E. et al. Tumor cells can escape DNA-damaging cisplatin through DNA endoreduplication and reversible polyploidy. Cell Biol. Int. 32(9), 1031–1043 (2008).CAS 
PubMed 
Article 

Google Scholar 
Zhang, S. et al. Generation of cancer stem-like cells through the formation of polyploid giant cancer cells. Oncogene 33(1), 116–128 (2014).CAS 
PubMed 
Article 

Google Scholar 
Lin, K. C. et al. The role of heterogeneous environment and docetaxel gradient in the emergence of polyploid, mesenchymal and resistant prostate cancer cells. Clin. Exp. Metastasis 36(2), 97–108 (2019).PubMed 
Article 

Google Scholar 
Lin, K.-C. et al. Epithelial and mesenchymal prostate cancer cell population dynamics on a complex drug landscape. Converg. Sci. Phys. Oncol. 3(4), 045001 (2017).PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
Boe, L. Mechanism for induction of adaptive mutations in Escherichia coli. Mol. Microbiol. 4(4), 597–601 (1990).CAS 
PubMed 
Article 

Google Scholar 
Cairns, J. Mutation and cancer: The antecedents to our studies of adaptive mutation. Genetics 148(4), 1433–1440 (1998).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Hall, B. G. Adaptive mutagenesis: A process that generates almost exclusively beneficial mutations. Genetica 102, 109 (1998).PubMed 
Article 

Google Scholar 
Waddington, C. H. Genetic assimilation of an acquired character. Evolution 7(2), 118–126 (1953).Article 

Google Scholar 
Waddington, C. H. Genetic assimilation. Adv. Genet. 10, 257–293 (1961).CAS 
PubMed 
Article 

Google Scholar 
Jablonka, E. V. A. & Raz, G. A. L. Transgenerational epigenetic inheritance: Prevalence, mechanisms, and implications for the study of heredity and evolution. Q. Rev. Biol. 84(2), 131–176 (2009).PubMed 
Article 

Google Scholar 
Steele, E. J. & Pollard, J. W. Hypothesis: Somatic hypermutation by gene conversion via the error prone DNA(longrightarrow )RNA(longrightarrow )DNA information loop. Mol. Immunol. 24(6), 667–673 (1987).CAS 
PubMed 
Article 

Google Scholar 
Steele, E. J. Somatic hypermutation in immunity and cancer: Critical analysis of strand-biased and codon-context mutation signatures. DNA Repair 45, 1–24 (2016).CAS 
PubMed 
Article 

Google Scholar 
Steele, E. J. Somatic Selection and Adaptive Evolution (Springer, US, 1979).
Google Scholar 
Steele, E. J., Lindley, R. A. & Blanden, R. V. Lamarck’s Signature (Perseus Books, 1998).
Google Scholar 
Foster, P. L. Adaptive mutation: Implications for evolution. Bioessays 22, 1067–1074 (2000).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
McCutcheon, J. P. & Moran, N. A. Extreme genome reduction in symbiotic bacteria. Nat. Rev. Microbiol. 10(1), 13–26 (2012).CAS 
Article 

Google Scholar 
Badyaev, A. V. Stress-induced variation in evolution: From behavioural plasticity to genetic assimilation. Proc. R. Soc. B Biol. Sci. 272, 877–886 (2005).Article 

Google Scholar 
Bateman, K. G. The genetic assimilation of four venation phenocopies. J. Genet. 56(3), 443–474 (1959).Article 

Google Scholar 
Milkman, R. D. The genetic basis of natural variation. VI. Selection of a crossveinless strain of Drosophila by phenocopying at high temperature. Genetics 51(1), 87 (1965).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Waddington, C. H. Genetic assimilation of the bithorax phenotype. Evolution 10(1), 1–13 (1956).Article 

Google Scholar 
Godoy, O., Saldaña, A., Fuentes, N., Valladares, F. & Gianoli, E. Forests are not immune to plant invasions: Phenotypic plasticity and local adaptation allow Prunella vulgaris to colonize a temperate evergreen rainforest. Biol. Invasions 13(7), 1615–1625 (2011).Article 

Google Scholar 
Schlichting, C. D. & Wund, M. A. Phenotypic plasticity and epigenetic marking: An assessment of evidence for genetic accommodation. Evolution 68(3), 656–672 (2014).PubMed 
Article 

Google Scholar 
Otaki, J. M., Hiyama, A., Iwata, M. & Kudo, T. Phenotypic plasticity in the range-margin population of the lycaenid butterfly Zizeeria maha. BMC Evol. Biol. 10(1), 1–13 (2010).Article 

Google Scholar 
Aubret, F. & Shine, R. Genetic assimilation and the postcolonization erosion of phenotypic plasticity in island tiger snakes. Curr. Biol. 19(22), 1932–1936 (2009).CAS 
PubMed 
Article 

Google Scholar 
Losos, J. B., Irschick, D. J. & Schoener, T. W. Adaptation and constraint in the evolution of specialization of Bahamian Anolis lizards. Evolution 48(6), 1786–1798 (1994).PubMed 
Article 

Google Scholar 
Losos, J. B. et al. Evolutionary implications of phenotypic plasticity in the hindlimb of the lizard Anolis sagrei. Evolution 54(1), 301–305 (2000).CAS 
PubMed 

Google Scholar 
Sword, G. A. Density-dependent warning coloration. Nature 397(6716), 217 (1999).ADS 
CAS 
Article 

Google Scholar 
Sword, G. A. A role for phenotypic plasticity in the evolution of aposematism. Proc. R. Soc. B Biol. Sci. 269(1501), 1639–1644 (2002).Article 

Google Scholar 
Clausen, J. & Hiesey, W. M. The balance between coherence and variation in evolution. Proc. Natl. Acad. Sci. 46(4), 494–506 (1960).ADS 
CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Gurevitch, J. Variation in leaf dissection and leaf energy budgets among populations of Achillea from an altitudinal gradient. Am. J. Bot. 75(9), 1298–1306 (1988).Article 

Google Scholar 
Gurevitch, J. & Schuepp, P. H. Boundary layer properties of highly dissected leaves: An investigation using an electrochemical fluid tunnel. Plant Cell Environ. 13(8), 783–792 (1990).Article 

Google Scholar 
Gurevitch, J. Sources of variation in leaf shape among two populations of Achillea lanulosa. Genetics 130(2), 385–394 (1992).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Foster, P. L. Stress-induced mutagenesis in bacteria. Crit. Rev. Biochem. Mol. Biol. 42(5), 373–397 (2007).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Soppa, J. Polyploidy in archaea and bacteria: About desiccation resistance, giant cell size, long-term survival, enforcement by a eukaryotic host and additional aspects. Microb. Physiol. 24, 409–419 (2014).ADS 
CAS 
Article 

Google Scholar 
Bastide, A. & David, A. The ribosome, (slow) beating heart of cancer (stem) cell. Oncogenesis 7(4), 1–13 (2018).CAS 
Article 

Google Scholar 
Cairns, J., Overbaugh, J. & Miller, S. The origin of mutants. Nature 335, 142–145 (1988).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Foster, P. L. Adaptive mutation: The uses of adversity. Annu. Rev. Microbiol. 47, 467–504. https://doi.org/10.1146/annurev.mi.47.100193.002343 (2003).Article 

Google Scholar 
Lenski, R. E. & Mittler, J. E. The directed mutation controversy and neo-Darwinism. Science 259(5092), 188–194 (1993).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Lenski, R. E. & Sniegowski, P. D. “Adaptive mutation’’: The debate goes on. Science 269, 285–288 (1995).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Noller, H. F., Hoffarth, V. & Zimniak, L. Unusual resistance of peptidyl transferase to protein extraction procedures. Science 256(5062), 1416–1419 (1992).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Pribis, J. P. et al. Gamblers: An antibiotic-induced evolvable cell subpopulation differentiated by reactive-oxygen-induced general stress response. Mol. Cell 74(4), 785–800 (2019).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Silvera, D., Formenti, S. C. & Schneider, R. J. Translational control in cancer. Nat. Rev. Cancer 10(4), 254–266 (2010).CAS 
PubMed 
Article 

Google Scholar 
Shcherbakov, D. et al. Ribosomal mistranslation leads to silencing of the unfolded protein response and increased mitochondrial biogenesis. Commun. Biol. 2(1), 1–16 (2019).CAS 
Article 

Google Scholar 
Truitt, M. L. & Ruggero, D. New frontiers in translational control of the cancer genome. Nat. Rev. Cancer 16(5), 288–304 (2016).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Alphey, L. S., Crisanti, A., Randazzo, F. & Akbari, O. S. Opinion: Standardizing the definition of gene drive. Proc. Natl. Acad. Sci. USA 117(49), 30864 (2020).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Champer, J., Buchman, A. & Akbari, O. S. Cheating evolution: Engineering gene drives to manipulate the fate of wild populations. Nat. Rev. Genet. 17, 146–159 (2016).CAS 
PubMed 
Article 

Google Scholar 
Champer, S. E. et al. Modeling CRISPR gene drives for suppression of invasive rodents using a supervised machine learning framework. PLOS Comput. Biol. 17(12), e1009660 (2021).ADS 
CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Deredec, A., Burt, A. & Godfray, H. C. J. The population genetics of using homing endonuclease genes in vector and pest management. Genetics 179(4), 2013–2026 (2008).PubMed 
PubMed Central 
Article 

Google Scholar 
Heffel, M. G. & Finnigan, G. C. Mathematical modeling of self-contained CRISPR gene drive reversal systems. Sci. Rep. 9(1), 1–10 (2019).Article 
CAS 

Google Scholar 
Leftwich, P. T. et al. Recent advances in threshold-dependent gene drives for mosquitoes. Biochem. Soc. Trans. 46, 1203–1212 (2018).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Nijhout, H. F., Kudla, A. M. & Hazelwood, C. C. Genetic assimilation and accommodation: Models and mechanisms. Curr. Top. Dev. Biol. 141, 337–369 (2021).PubMed 
Article 

Google Scholar 
Noble, C., Adlam, B., Church, G. M., Esvelt, K. M. & Nowak, M. A. Current CRISPR gene drive systems are likely to be highly invasive in wild populations. eLife 7, e33423 (2018).PubMed 
PubMed Central 
Article 

Google Scholar 
Novozhilov, A. S., Karev, G. P. & Koonin, E. V. Mathematical modeling of evolution of horizontally transferred genes. Mol. Biol. Evol. 22(8), 1721–1732 (2005).CAS 
PubMed 
Article 

Google Scholar 
Pigliucci, M. & Murren, C. J. Perspective: Genetic assimilation and a possible evolutionary paradox: Can macroevolution sometimes be so fast as to pass us by?. Evolution 57, 1455–1464 (2003).PubMed 
Article 

Google Scholar 
Hammerstein, P. Darwinian adaptation, population genetics and the streetcar theory of evolution. J. Math. Biol. 34(5–6), 511–532 (1996).CAS 
PubMed 
MATH 
Article 

Google Scholar 
Dieckmann, U. Coevolutionary Dynamics of Stochastic Replicator Systems (Central Library of the Research Center Jülich, 1994).
Google Scholar 
Dieckmann, U., Marrow, P. & Law, R. Evolutionary cycling in predator-prey interactions: population dynamics and the red queen. J. Theor. Biol. 176(1), 91–102 (1995).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Dieckmann, U. & Law, R. The dynamical theory of coevolution: a derivation from stochastic ecological processes. J. Math. Biol. 34, 579–612 (1996).MathSciNet 
CAS 
PubMed 
MATH 
Article 

Google Scholar 
Metz, J. A. J., Nisbet, R. M. & Geritz, S. A. H. How should we define ‘fitness’ for general ecological scenarios?. Trends Ecol. Evol. 7(6), 198–202 (1992).CAS 
PubMed 
Article 

Google Scholar 
Goldschmidt, R. Some aspects of evolution. Science 78(2033), 539–547 (1933).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Vincent, T. L., Cohen, Y. & Brown, J. S. Evolution via strategy dynamics. Theor. Popul. Biol. 44(2), 149–176 (1993).MATH 
Article 

Google Scholar 
Bell, G. Evolutionary rescue. Annu. Rev. Ecol. Evol. Syst. 48, 605–627 (2017).Article 

Google Scholar  More

Lombard, F. et al. Consistent quantitative observations of planktonic ecosystems. Front. Mar. Sci. 6, 196. https://doi.org/10.3389/fmars.2019.00196 (2019).Article 

Google Scholar 
Sieracki, M. E., et al. Optical plankton imaging and analysis systems for ocean observation. Proceedings of OceanObs’09: Sustained Ocean Observations and Information for Society, 878–885 (2010). https://doi.org/10.5270/OceanObs09.cwp.81.Irisson, J.-O., Ayata, S.-D., Lindsay, D. J., Karp-Boss, L. & Stemmann, L. Machine learning for the study of plankton and marine snow from images. Ann. Rev. Mar. Sci. 14(1), 277. https://doi.org/10.1146/annurev-marine-041921-013023 (2022).Article 
PubMed 

Google Scholar 
Mars Brisbin, M., Brunner, O. D., Grossmann, M. M. & Mitarai, S. Paired high-throughput, in situ imaging and high-throughput sequencing illuminate acantharian abundance and vertical distribution. Limnol. Oceanogr. 65(12), 2953–2965. https://doi.org/10.1002/lno.11567 (2020).ADS 
CAS 
Article 

Google Scholar 
Benfield, M. et al. RAPID: Research on automated plankton identification. Oceanography 20(2), 172–187. https://doi.org/10.5670/oceanog.2007.63 (2007).Article 

Google Scholar 
Colin, S. et al. Quantitative 3D-imaging for cell biology and ecology of environmental microbial eukaryotes. Elife 6, e26066. https://doi.org/10.7554/eLife.26066 (2017).Article 
PubMed 
PubMed Central 

Google Scholar 
Kim, M. K. Principles and techniques of digital holographic microscopy. J. Photonics Energy. 1, 018005. https://doi.org/10.1117/6.0000006 (2010).Article 

Google Scholar 
Tahara, T., Quan, X., Otani, R., Takaki, Y. & Matoba, O. Digital holography and its multidimensional imaging applications: A review. Microscopy 67(2), 55–67. https://doi.org/10.1093/jmicro/dfy007 (2018).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Jericho, S. K., Garcia-Sucerquia, J. F. W., Jericho, M. H. & Kreuzer, H. J. Submersible digital in-line holographic microscope. Rev. Sci. Instrum. 77(4), 043706. https://doi.org/10.1063/1.2193827 (2006).ADS 
CAS 
Article 

Google Scholar 
Bochdansky, A. B., Jericho, M. H. & Herndl, G. J. Development and deployment of a point-source digital inline holographic microscope for the study of plankton and particlesto a depth of 6000 m. Limnol. Oceanogr: Methods 11, 28–40 (2013).Article 

Google Scholar 
Yourassowsky, C. & Dubois, F. High throughput holographic imaging-in-flow for the analysis of a wide plankton size range. Opt. Express 22(6), 6661. https://doi.org/10.1364/OE.22.006661 (2014).ADS 
Article 
PubMed 

Google Scholar 
Jericho, M. H. & Kreuzer, H. J. Point source digital in-line holographic microscopy. In Coherent Light Microscopy (eds Ferraro, P. et al.) 3–30 (Springer, 2011).Chapter 

Google Scholar 
Kanka, M., Riesenberg, R. & Kreuzer, H. J. Reconstruction of high-resolution holographic microscopic images. Opt. Lett. 34(8), 1162. https://doi.org/10.1364/OL.34.001162 (2009).ADS 
CAS 
Article 
PubMed 

Google Scholar 
Jericho, M. H., Kreuzer, H. J., Kanka, M. & Riesenberg, R. Quantitative phase and refractive index measurements with point-source digital in-line holographic microscopy. Appl. Opt. 51(10), 1503. https://doi.org/10.1364/AO.51.001503 (2012).ADS 
CAS 
Article 
PubMed 

Google Scholar 
Wu, Y. & Ozcan, A. Lensless digital holographic microscopy and its applications in biomedicine and environmental monitoring. Methods 136, 4–16 (2018).CAS 
Article 

Google Scholar 
Sun, H. et al. digital holography for studies of marine plankton. Philos. Trans. R. Soc. A. 366, 1789–1806 (2008).ADS 
CAS 
Article 

Google Scholar 
Bianco, V. et al. microplastic identification via holographic imaging and machine learning. Adv. Intell. Syst. 2(2), 1900153. https://doi.org/10.1002/aisy.201900153 (2020).Article 

Google Scholar 
Guo, B. et al. Automated plankton classification from holographic imagery with deep convolutional neural networks. Limnol. Oceanogr. 19(1), 21–36. https://doi.org/10.1002/lom3.10402 (2021).Article 

Google Scholar 
Nayak, A. R., Malkiel, E., McFarland, M. N., Twardowski, M. S. & Sullivan, J. M. A Review of holography in the aquatic sciences: In situ characterization of particles, plankton, and small scale biophysical interactions. Front. Mar. Sci. 7, 572147. https://doi.org/10.3389/fmars.2020.572147 (2021).Article 

Google Scholar 
Di Bella, J. M., Bao, Y., Gloor, G. B., Burton, J. P. & Reid, G. High throughput sequencing methods and analysis for microbiome research. J. Microbiol. Methods 95(3), 401–414. https://doi.org/10.1016/j.mimet.2013.08.011 (2013).CAS 
Article 
PubMed 

Google Scholar 
Stoeck, T. et al. Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water. Mol. Ecol. 19, 21–31. https://doi.org/10.1111/j.1365-294X.2009.04480.x (2010).CAS 
Article 
PubMed 

Google Scholar 
de Vargas, C. et al. Eukaryotic plankton diversity in the sunlit ocean. Science 348(6237), 1261605–1261605. https://doi.org/10.1126/science.1261605 (2015).CAS 
Article 
PubMed 

Google Scholar 
Lima-Mendez, G. et al. Determinants of community structure in the global plankton interactome. Science 348(6237), 1262073–1262073. https://doi.org/10.1126/science.1262073 (2015).CAS 
Article 
PubMed 

Google Scholar 
Santoferrara, L. et al. Perspectives from ten years of protist studies by high-throughput metabarcoding. J. Eukaryot. Microbiol. 67(5), 612–622. https://doi.org/10.1111/jeu.12813 (2020).Article 
PubMed 

Google Scholar 
Eickbush, T. H. & Eickbush, D. G. Finely orchestrated movements: evolution of the ribosomal RNA genes. Genetics 175(2), 477–485. https://doi.org/10.1534/genetics.107.071399 (2007).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Kirkham, A. R. et al. Basin-scale distribution patterns of photosynthetic picoeukaryotes along an Atlantic Meridional Transect: Marine photosynthetic picoeukaryote community structure. Environ. Microbiol. 13(4), 975–990. https://doi.org/10.1111/j.1462-2920.2010.02403.x (2011).CAS 
Article 
PubMed 

Google Scholar 
Decelle, J. et al. PhytoREF: A reference database of the plastidial 16S rRNA gene of photosynthetic eukaryotes with curated taxonomy. Mol. Ecol. Resour. 15(6), 1435–1445. https://doi.org/10.1111/1755-0998.12401 (2015).CAS 
Article 
PubMed 

Google Scholar 
Leray, M. & Knowlton, N. Censusing marine eukaryotic diversity in the twenty-first century. Phil. Trans. R. Soc. B. 371(1702), 20150331. https://doi.org/10.1098/rstb.2015.0331 (2016).Article 
PubMed 
PubMed Central 

Google Scholar 
Cowart, D. A. et al. Metabarcoding is powerful yet still blind: A comparative analysis of morphological and molecular surveys of seagrass communities. PLoS ONE 10(2), e0117562. https://doi.org/10.1371/journal.pone.0117562 (2015).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Stefanni, S. et al. Multi-marker metabarcoding approach to study mesozooplankton at basin scale. Sci. Rep. 8(1), 12085. https://doi.org/10.1038/s41598-018-30157-7 (2018).ADS 
CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Pappalardo, P. et al. The role of taxonomic expertise in interpretation of metabarcoding studies. ICES J. Mar. Sci. https://doi.org/10.1093/icesjms/fsab082 (2021).Article 

Google Scholar 
Gloor, G. B., Macklaim, J. M., Pawlowsky-Glahn, V. & Egozcue, J. J. Microbiome datasets are compositional: And this is not optional. Front. Microbiol. 8, 2224. https://doi.org/10.3389/fmicb.2017.02224 (2017).Article 
PubMed 
PubMed Central 

Google Scholar 
Zhu, F., Massana, R., Not, F., Marie, D. & Vaulot, D. Mapping of picoeucaryotes in marine ecosystems with quantitative PCR of the 18S rRNA gene. FEMS Microbiol. Ecol. 52(1), 79–92. https://doi.org/10.1016/j.femsec.2004.10.006 (2005).CAS 
Article 
PubMed 

Google Scholar 
Sargent, E. C. et al. Evidence for polyploidy in the globally important diazotroph Trichodesmium. FEMS Microbiol. Lett. 363(21), 244. https://doi.org/10.1093/femsle/fnw244 (2016).CAS 
Article 

Google Scholar 
Gong, W. & Marchetti, A. Estimation of 18S gene copy number in marine eukaryotic plankton using a next-generation sequencing approach. Front. Mar. Sci. 6, 219. https://doi.org/10.3389/fmars.2019.00219 (2019).Article 

Google Scholar 
Biard, T. et al. Biogeography and diversity of collodaria (radiolaria) in the global ocean. ISME J. 11, 1331–1344 (2017).Article 

Google Scholar 
Callahan, B. J., McMurdie, P. J. & Holmes, S. P. Exact sequence variants should replace operational taxonomic units in marker-gene data analysis. ISME J. 11(12), 2639–2643. https://doi.org/10.1038/ismej.2017.119 (2017).Article 
PubMed 
PubMed Central 

Google Scholar 
Behrenfeld, M. J. et al. The North Atlantic aerosol and marine ecosystem study (NAAMES): Science motive and mission overview. Front. Mar. Sci. 6, 122. https://doi.org/10.3389/fmars.2019.00122 (2019).Article 

Google Scholar 
Bolaños, L. M. et al. Seasonality of the microbial community composition in the North Atlantic. Front. Mar. Sci. 8, 624164. https://doi.org/10.3389/fmars.2021.624164 (2021).Article 

Google Scholar 
Aitchison, J. The statistical analysis of compositional data. J. R. Stat. Soc. B 44(2), 139–160. https://doi.org/10.1111/j.2517-6161.1982.tb01195.x (1982).MathSciNet 
Article 
MATH 

Google Scholar 
Decelle, J. & Not, F. Acantharia. ELS, 1–10 (2015). https://doi.org/10.1002/9780470015902.a0002102.pub2.Yu, L., An, Y. & Cai, L. Numerical reconstruction of digital holograms with variable viewing angles. Opt. Express 10(22), 1250. https://doi.org/10.1364/OE.10.001250 (2002).ADS 
Article 
PubMed 

Google Scholar 
Della Penna, A. & Gaube, P. Overview of (sub)mesoscale Ocean dynamics for the NAAMES field program. Front. Mar. Sci. 6, 384. https://doi.org/10.3389/fmars.2019.00384 (2019).Article 

Google Scholar 
Sverdrup, H. U. Oceanography for Meteorologists (Prentice Hall, 1942).Book 

Google Scholar 
Mahadevan, A. The impact of submesoscale physics on primary productivity of plankton. Annu. Rev. Mar. Sci. 8(1), 161–184. https://doi.org/10.1146/annurev-marine-010814-015912 (2016).ADS 
Article 

Google Scholar 
Fratantoni, P. S. & Pickart, R. S. The Western North Atlantic shelfbreak current system in summer. J. Phys. Oceanogr. 37(10), 2509–2533. https://doi.org/10.1175/JPO3123.1 (2007).ADS 
Article 

Google Scholar 
Bolaños, L. M. et al. Small phytoplankton dominate western North Atlantic biomass. ISME J. 14(7), 1663–1674. https://doi.org/10.1038/s41396-020-0636-0 (2020).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Kramer, S. J., Siegel, D. A. & Graff, J. R. Phytoplankton community composition determined from co-variability among phytoplankton pigments from the NAAMES field campaign. Front. Mar. Sci. 7, 215. https://doi.org/10.3389/fmars.2020.00215 (2020).Article 

Google Scholar 
Faure, E. et al. Mixotrophic protists display contrasted biogeographies in the global ocean. ISME J. 13(4), 1072–1083. https://doi.org/10.1038/s41396-018-0340-5 (2019).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Fratantoni, P. S. & McCartney, M. S. Freshwater export from the labrador current to the North Atlantic Current at the tail of the grand banks of Newfoundland. Deep Sea Res. I. 57(2), 258–283. https://doi.org/10.1016/j.dsr.2009.11.006 (2010).Article 

Google Scholar 
Torti, A., Lever, M. A. & Jørgensen, B. B. Origin, dynamics, and implications of extracellular DNA pools in marine sediments. Mar. Genom. 24, 185–196. https://doi.org/10.1016/j.margen.2015.08.007 (2015).Article 

Google Scholar 
Jian, C., Salonen, A. & Korpela, K. Commentary: How to count our microbes? The effect of different quantitative microbiome profiling approaches. Front. Cell. Infect. Microbiol. 11, 627910. https://doi.org/10.3389/fcimb.2021.627910 (2021).Article 
PubMed 
PubMed Central 

Google Scholar 
Djurhuus, A. et al. Evaluation of marine zooplankton community structure through environmental DNA metabarcoding: Metabarcoding zooplankton from eDNA. Limnol. Oceanogr. Methods 16(4), 209–221. https://doi.org/10.1002/lom3.10237 (2018).Article 
PubMed 
PubMed Central 

Google Scholar 
del Campo, J. et al. The others: Our biased perspective of eukaryotic genomes. Trends Ecol. Evol. 29(5), 252–259. https://doi.org/10.1016/j.tree.2014.03.006 (2014).Article 
PubMed 
PubMed Central 

Google Scholar 
Karst, S. M. et al. Retrieval of a million high-quality, full-length microbial 16S and 18S rRNA gene sequences without primer bias. Nat. Biotech. 36(2), 190–195. https://doi.org/10.1038/nbt.4045 (2018).CAS 
Article 

Google Scholar 
Johnson, J. S. et al. Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis. Nat. Commun. 10(1), 5029. https://doi.org/10.1038/s41467-019-13036-1 (2019).ADS 
CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Callahan, B. J. et al. High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution. Nucleic Acids Res. 47(18), e103–e103. https://doi.org/10.1093/nar/gkz569 (2019).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Lin, Y., Gifford, S., Ducklow, H., Schofield, O. & Cassar, N. Towards quantitative microbiome community profiling using internal standards. Appl. Environ. Microbiol. 85(5), 18. https://doi.org/10.1128/AEM.02634-18 (2019).Article 

Google Scholar 
Vogt, M. et al. Global marine plankton functional type biomass distributions: Phaeocystis spp. Earth Syst. Sci. Data 5, 405–443. https://doi.org/10.5194/essdd-5-405-2012 (2012).ADS 
Article 

Google Scholar 
MacNeil, L., Missan, S., Luo, J., Trappenberg, T. & LaRoche, J. Plankton classification with high-throughput submersible holographic microscopy and transfer learning. BMC Ecol. Evol. 21(1), 123. https://doi.org/10.1186/s12862-021-01839-0 (2021).Article 
PubMed 
PubMed Central 

Google Scholar 
Pan, J., del Campo, J. & Keeling, P. J. Reference tree and environmental sequence diversity of labyrinthulomycetes. J. Eukary. Microbiol. 64(1), 88–96. https://doi.org/10.1111/jeu.12342 (2017).Article 

Google Scholar 
Bochdansky, A. B., Clouse, M. A. & Herndl, G. J. Eukaryotic microbes, principally fungi and labyrinthulomycetes, dominate biomass on bathypelagic marine snow. ISME J. 11(2), 362–373. https://doi.org/10.1038/ismej.2016.113 (2017).Article 
PubMed 

Google Scholar 
Xie, N., Hunt, D. E., Johnson, Z. I., He, Y. & Wang, G. Annual partitioning patterns of Labyrinthulomycetes protists reveal their multifaceted role in marine microbial food webs. Appl. Environ. Microbiol. https://doi.org/10.1128/AEM.01652-20 (2021).Article 
PubMed 
PubMed Central 

Google Scholar 
Walcutt, N. L. et al. Assessment of holographic microscopy for quantifying marine particle size and concentration. Limnol. Oceanogr. Methods 3, 10379. https://doi.org/10.1002/lom3.10379 (2020).Article 

Google Scholar 
Axler, K. et al. Fine-scale larval fish distributions and predator-prey dynamics in a coastal river-dominated ecosystem. Mar. Ecol. Prog. Ser. 650, 37–61. https://doi.org/10.3354/meps13397 (2020).ADS 
Article 

Google Scholar 
Trudnowska, E. et al. Marine snow morphology illuminates the evolution of phytoplankton blooms and determines their subsequent vertical export. Nat. Commun. 12(1), 2816. https://doi.org/10.1038/s41467-021-22994-4 (2021).ADS 
CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
González, P. et al. Automatic plankton quantification using deep features. J. Plankton Res. 41(4), 449–463. https://doi.org/10.1093/plankt/fbz023 (2019).Article 

Google Scholar 
Briseño-Avena, C. et al. Three-dimensional cross-shelf zooplankton distributions off the Central Oregon Coast during anomalous oceanographic conditions. Prog. Oceanogr. 188, 102436. https://doi.org/10.1016/j.pocean.2020.102436 (2020).Article 

Google Scholar 
Biard, T. et al. In situ imaging reveals the biomass of giant protists in the global ocean. Nature 532, 504–507 (2016).ADS 
CAS 
Article 

Google Scholar 
Orenstein, E. C. et al. The scripps plankton camera system: A framework and platform for in situ microscopy. Limnol. Oceanogr. Methods 18(11), 681–695. https://doi.org/10.1002/lom3.10394 (2020).Article 

Google Scholar 
Fowler, B. L. et al. Dynamics and functional diversity of the smallest phytoplankton on the Northeast US Shelf. PNAS 117(22), 12215–12221. https://doi.org/10.1073/pnas.1918439117 (2020).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Tréguer, P. et al. Influence of diatom diversity on the ocean biological carbon pump. Nat. Geosci. 11(1), 27–37. https://doi.org/10.1038/s41561-017-0028-x (2018).ADS 
CAS 
Article 

Google Scholar 
Ryabov, A. et al. Shape matters: The relationship between cell geometry and diversity in phytoplankton. Ecol. Lett. 24(4), 847–861. https://doi.org/10.1111/ele.13680 (2021).MathSciNet 
Article 
PubMed 

Google Scholar 
Keeling, P. J. & del Campo, J. marine protists are not just big bacteria. Curr. Biol. 27(11), R541–R549. https://doi.org/10.1016/j.cub.2017.03.075 (2017).CAS 
Article 
PubMed 

Google Scholar 
Sgubin, G., Swingedouw, D., Drijfhout, S., Mary, Y. & Bennabi, A. Abrupt cooling over the North Atlantic in modern climate models. Nat. Commun. 8(1), 14375. https://doi.org/10.1038/ncomms14375 (2017).CAS 
Article 
PubMed Central 

Google Scholar 
Desbruyères, D., Chafik, L. & Maze, G. A shift in the ocean circulation has warmed the subpolar North Atlantic Ocean since 2016. Commun. Earth Environ. 2(1), 48. https://doi.org/10.1038/s43247-021-00120-y (2021).ADS 
Article 

Google Scholar 
Mitchell, M. R. et al. Atlantic zone monitoring program protocol. Can. Tech. Rep. Hydrogr. Ocean Sci. 223, 1–23 (2002).
Google Scholar 
Li, W. K. W., Glen Harrison, W. & Head, E. J. H. Coherent assembly of phytoplankton communities in diverse temperate ocean ecosystems. Proc. R. Soc. B. 273(1596), 1953–1960. https://doi.org/10.1098/rspb.2006.3529 (2006).Article 
PubMed 
PubMed Central 

Google Scholar 
Richardson, P. L. Florida current, gulf stream, and labrador current. In Encyclopedia of Ocean Sciences (ed. Steele, J. H.) 1054–1064 (Academic Press, 2001). https://doi.org/10.1006/rwos.2001.0357.Chapter 

Google Scholar 
Henson, S. A., Dunne, J. P. & Sarmiento, J. L. Decadal variability in North Atlantic phytoplankton blooms. J. Geophys. Res. 114(C4), C04013. https://doi.org/10.1029/2008JC005139 (2009).ADS 
CAS 
Article 

Google Scholar 
Han, G., Lu, Z., Wang, Z., Helbig, J. & Chen, N. Seasonal variability of the labrador current and shelf circulation off Newfoundland. J. Geophys. Res. 113, 10. https://doi.org/10.1029/2007JC004376 (2008).Article 

Google Scholar 
Pante, E. & Simon-Bouhet, B. marmap: A package for importing, plotting and analyzing bathymetric and topographic data in R. PLoS ONE 8(9), e73051. https://doi.org/10.1371/journal.pone.0073051 (2013).ADS 
CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Kelley, D. “The Oce Package” In Oceanographic Analysis with R 91–101 (Springer, 2018).Book 

Google Scholar 
Oksanen, J., et al. vegan: Community Ecology Package. R package version 2.5-7 (2020). https://CRAN.R-project.org/package=vegan.Tomas, C. R. Identifying Marine Phytoplankton (Academic Press Inc, 1997).
Google Scholar 
Comeau, A. M., Li, W. K. W., Tremblay, J. -É., Carmack, E. C. & Lovejoy, C. Arctic ocean microbial community structure before and after the 2007 record sea ice minimum. PLoS ONE 6(11), e27492. https://doi.org/10.1371/journal.pone.0027492 (2011).ADS 
CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Parada, A. E., Needham, D. M. & Fuhrman, J. A. Every base matters: Assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples: Primers for marine microbiome studies. Environ. Microbiol. 18(5), 1403–1414. https://doi.org/10.1111/1462-2920.13023 (2016).CAS 
Article 
PubMed 

Google Scholar 
Walters, W. et al. Improved bacterial 16S rRNA gene (V4 and V4–5) and fungal internal transcribed spacer marker gene primers for microbial community surveys. MSystems https://doi.org/10.1128/mSystems.00009-15 (2016).Article 
PubMed 

Google Scholar 
Comeau, A. M., Douglas, G. M. & Langille, M. G. I. Microbiome helper: A custom and streamlined workflow for microbiome research. MSystems 2(1), e00127-e216. https://doi.org/10.1128/mSystems.00127-16 (2017).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Bolyen, E. et al. Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2. Nat. Biotech. 37(8), 852–857. https://doi.org/10.1038/s41587-019-0209-9 (2019).CAS 
Article 

Google Scholar 
Amir, A. et al. Deblur rapidly resolves single-nucleotide community sequence patterns. MSystems 2(2), e00191-e216. https://doi.org/10.1128/mSystems.00191-16 (2017).Article 
PubMed 
PubMed Central 

Google Scholar 
Guillou, L. et al. The protist ribosomal reference database (PR2): A catalog of unicellular eukaryote small sub-unit rRNA sequences with curated taxonomy. Nucleic Acids Res. 41(D1), D597–D604. https://doi.org/10.1093/nar/gks1160 (2013).CAS 
Article 
PubMed 

Google Scholar 
Mohsen, A., Park, J., Chen, Y.-A., Kawashima, H. & Mizuguchi, K. Impact of quality trimming on the efficiency of reads joining and diversity analysis of Illumina paired-end reads in the context of QIIME1 and QIIME2 microbiome analysis frameworks. BMC Bioinform. 20(1), 581. https://doi.org/10.1186/s12859-019-3187-5 (2019).Article 

Google Scholar 
Bokulich, N. A. et al. Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2’s q2-feature-classifier plugin. Microbiome 6(1), 90. https://doi.org/10.1186/s40168-018-0470-z (2018).MathSciNet 
Article 
PubMed 
PubMed Central 

Google Scholar 
Quast, C. et al. The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools. Nucleic Acids Res. 41(D1), D590–D596. https://doi.org/10.1093/nar/gks1219 (2012).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
R Core Team. R: A Language and Environment for Statistical Computing. (R Foundation for Statistical Computing, 2021). https://www.R-project.org/.McMurdie, P. J. & Holmes, S. phyloseq: An R package for reproducible interactive analysis and graphics of microbiome census data. PLoS ONE 8(4), e61217. https://doi.org/10.1371/journal.pone.0061217 (2013).ADS 
CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Willis, A. & Bunge, J. Estimating diversity via frequency ratios: estimating diversity via ratios. Biometrics 71(4), 1042–1049. https://doi.org/10.1111/biom.12332 (2015).MathSciNet 
Article 
PubMed 
MATH 

Google Scholar 
Willis, A. D. Rarefaction, alpha diversity, and statistics. Front. Microbiol. 10, 2407. https://doi.org/10.3389/fmicb.2019.02407 (2019).Article 
PubMed 
PubMed Central 

Google Scholar 
Quinn, T. P. et al. A field guide for the compositional analysis of any-omics data. GigaScience 8(9), 107. https://doi.org/10.1093/gigascience/giz107 (2019).CAS 
Article 

Google Scholar 
Silverman, J. D., Roche, K., Mukherjee, S. & David, L. A. Naught all zeros in sequence count data are the same. Comput. Struct. Biotech. J. 18, 2789–2798. https://doi.org/10.1016/j.csbj.2020.09.014 (2020).CAS 
Article 

Google Scholar 
Anderson, M. J. A new method for non-parametric multivariate analysis of variance. Austral. Ecol. 26, 32–46 (2001).
Google Scholar  More

Sun, C., Zhang, J., Ma, Q., Chen, Y. & Ju, H. Polycyclic aromatic hydrocarbons (PAHs) in water and sediment from a river basin: Sediment–water partitioning, source identification and environmental health risk assessment. Environ. Geochem. Health 39, 63–74 (2017).CAS 
PubMed 
Article 

Google Scholar 
Savari, M. & Shokati Amghani, M. Factors influencing farmers’ adaptation strategies in confronting the drought in Iran. Environ. Dev. Sustain. 2020 234 23, 4949–4972 (2020).Article 

Google Scholar 
Kumar Singh, P., Dey, P., Kumar Jain, S. & Mujumdar, P. P. Hydrology and water resources management in ancient India. Hydrol. Earth Syst. Sci. 24, 4691–4707 (2020).ADS 
Article 

Google Scholar 
Warner, L. A. & Diaz, J. M. Amplifying the Theory of Planned behavior with connectedness to water to inform impactful water conservation program planning and evaluation. J. Agric. Educ. Ext. 27, 229–253 (2021).Article 

Google Scholar 
Warner, L. A. Who conserves and who approves? Predicting water conservation intentions in urban landscapes with referent groups beyond the traditional ‘important others’. Urban For. Urban Green. 60, 127070 (2021).Article 

Google Scholar 
Savari, M., Eskandari Damaneh, H. & Eskandari Damaneh, H. Drought vulnerability assessment: Solution for risk alleviation and drought management among Iranian farmers. Int. J. Disaster Risk Reduct. 67, 102654 (2022).Article 

Google Scholar 
Eskandari Damaneh, H. et al. Testing possible scenario-based responses of vegetation under expected climatic changes in Khuzestan Province. Air Soil Water Res. https://doi.org/10.1177/1178622121101333214 (2021).Article 

Google Scholar 
Eskandari Damaneh, H., Khosravi, H., Habashi, K., Eskandari Damaneh, H. & Tiefenbacher, J. P. The impact of land use and land cover changes on soil erosion in western Iran. Nat. Hazards 110, 2185–2205 (2022).Article 

Google Scholar 
Savari, M., Abdeshahi, A., Gharechaee, H. & Nasrollahian, O. Explaining farmers’ response to water crisis through theory of the norm activation model: Evidence from Iran. Int. J. Disaster Risk Reduct. 60, 102284 (2021).Article 

Google Scholar 
Liu, J., Scanlon, B. R., Zhuang, J. & Varis, O. Food-energy-water nexus for multi-scale sustainable development. Resour. Conserv. Recycl. 154, 104565 (2020).Article 

Google Scholar 
Araya, F., Osman, K. & Faust, K. M. Perceptions versus reality: Assessing residential water conservation efforts in the household. Resour. Conserv. Recycl. 162, 105020 (2020).Article 

Google Scholar 
Omer, A., Elagib, N. A., Zhuguo, M., Saleem, F. & Mohammed, A. Water scarcity in the Yellow River Basin under future climate change and human activities. Sci. Total Environ. 749, 141446 (2020).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Aslam, S. et al. Sustainable model: Recommendations for water conservation strategies in a developing country through a psychosocial wellness program. Water (Switzerland) 13, 1–20 (2021).
Google Scholar 
Diaz, J., Odera, E. & Warner, L. Delving deeper: Exploring the influence of psycho-social wellness on water conservation behavior. J. Environ. Manag. 264, 110404 (2020).Article 

Google Scholar 
Fader, M., Shi, S., Von Bloh, W., Bondeau, A. & Cramer, W. Mediterranean irrigation under climate change: More efficient irrigation needed to compensate for increases in irrigation water requirements. Hydrol. Earth Syst. Sci. 20, 953–973 (2016).ADS 
CAS 
Article 

Google Scholar 
Brown, T. C., Mahat, V. & Ramirez, J. A. Adaptation to future water shortages in the United States caused by population growth and climate change. Earth’s Future 7, 219–234 (2019).ADS 
Article 

Google Scholar 
Lall, U., Josset, L. & Russo, T. A snapshot of the world’s groundwater challenges. Annu. Rev. Environ. Resour. 45, 171–194 (2020).Article 

Google Scholar 
Jin, J. et al. Impacts of climate change on hydrology in the Yellow River Source Region, China. J. Water Clim. Change 11, 916–930 (2020).Article 

Google Scholar 
Cochand, F., Brunner, P., Hunkeler, D., Rössler, O. & Holzkämper, A. Cross-sphere modelling to evaluate impacts of climate and land management changes on groundwater resources. Sci. Total Environ. 798, 148759 (2021).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Waha, K. et al. Climate change impacts in the Middle East and Northern Africa (MENA) region and their implications for vulnerable population groups. Reg. Environ. Change 17, 1623–1638 (2017).Article 

Google Scholar 
Boretti, A. & Rosa, L. Reassessing the projections of the World Water Development Report. npj Clean Water 2, 1–6 (2019).Article 

Google Scholar 
Fragaszy, S. R. et al. Drought monitoring in the Middle East and North Africa (MENA) region. Bull. Am. Meteorol. Soc. 101, 1148–1173 (2020).Article 

Google Scholar 
Tajeri moghadam, M., Raheli, H., Zarifian, S. & Yazdanpanah, M. The power of the health belief model (HBM) to predict water demand management: A case study of farmers’ water conservation in Iran. J. Environ. Manag. 263, 110388 (2020).Article 

Google Scholar 
Marston, L., Ao, Y., Konar, M., Mekonnen, M. M. & Hoekstra, A. Y. High-resolution water footprints of production of the United States. Water Resour. Res. 54, 2288–2316 (2018).ADS 
Article 

Google Scholar 
Savari, M. & Shokati Amghani, M. SWOT-FAHP-TOWS analysis for adaptation strategies development among small-scale farmers in drought conditions. Int. J. Disaster Risk Reduct. 67, 102695 (2022).Article 

Google Scholar 
Savari, M. & Moradi, M. The effectiveness of drought adaptation strategies in explaining the livability of Iranian rural households. Habitat Int. 124, 102560 (2022).Article 

Google Scholar 
Warner, L., Chaudhary, A. K., Rumble, J., Lamm, A. & Momol, E. Using audience segmentation to tailor residential irrigation water conservation programs. J. Agric. Educ. 58, 313–333 (2017).Article 

Google Scholar 
Tapsuwan, S., Cook, S. & Moglia, M. Willingness to pay for rainwater tank features: A post-drought analysis of Sydney water users. Water (Switzerland) 10, 1199 (2018).
Google Scholar 
Chubaka, C. E., Whiley, H., Edwards, J. W. & Ross, K. E. A review of roof harvested rainwater in Australia. J. Environ. Public Health 2018, 6471324 (2018).PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
Smith, H. M., Brouwer, S., Jeffrey, P. & Frijns, J. Public responses to water reuse—Understanding the evidence. J. Environ. Manag. 207, 43–50 (2018).CAS 
Article 

Google Scholar 
Addo, I. B., Thoms, M. C. & Parsons, M. Barriers and drivers of household water-conservation behavior: A profiling approach. Water (Switzerland) 10, 1794 (2018).
Google Scholar 
Jarrett, W. B. A survey of the influences on water conservation behavior in Pickens and Oconee counties (2015).Yazdanpanah, M., Forouzani, M., Abdeshahi, A. & Jafari, A. Investigating the effect of moral norm and self-identity on the intention toward water conservation among Iranian young adults. Water Policy 18, 73–90 (2016).Article 

Google Scholar 
Sabzali Parikhani, R., Sadighi, H. & Bijani, M. Ecological consequences of nanotechnology in agriculture: Researchers’ perspective. J. Agric. Sci. Technol. 20, 205–219 (2018).
Google Scholar 
Moglia, M., Cook, S. & Tapsuwan, S. Promoting water conservation: Where to from here?. Water (Switzerland) 10, 1510 (2018).
Google Scholar 
Savari, M. & Zhoolideh, M. The role of climate change adaptation of small-scale farmers on the households food security level in the west of Iran. Dev. Pract. 31, 650–664 (2021).Article 

Google Scholar 
Bennett, N. J. et al. Conservation social science: Understanding and integrating human dimensions to improve conservation. Biol. Conserv. 205, 93–108 (2017).Article 

Google Scholar 
Kumar Chaudhary, A., Lamm, A. & Warner, L. Using cognitive dissonance to theoretically explain water conservation intentions. J. Agric. Educ. 59, 194–210 (2018).Article 

Google Scholar 
Russell, S. V. & Knoeri, C. Exploring the psychosocial and behavioural determinants of household water conservation and intention. Int. J. Water Resour. Dev. 36, 940–955 (2020).Article 

Google Scholar 
Savari, M., Yazdanpanah, M. & Rouzaneh, D. Factors affecting the implementation of soil conservation practices among Iranian farmers. Sci. Rep. 12, 1–13 (2022).Article 
CAS 

Google Scholar 
Savari, M., Zhoolideh, M. & Khosravipour, B. Explaining pro-environmental behavior of farmers: A case of rural Iran. Curr. Psychol. https://doi.org/10.1007/S12144-021-02093-9 (2021).Article 

Google Scholar 
Lee, M. & Tansel, B. Water conservation quantities vs customer opinion and satisfaction with water efficient appliances in Miami, Florida. J. Environ. Manag. 128, 683–689 (2013).Article 

Google Scholar 
Yazdanpanah, M., Klein, K., Zobeidi, T., Sieber, S. & Löhr, K. Why have economic incentives failed to convince farmers to adopt drip irrigation in southwestern Iran?. Sustainability 14, 1–15 (2022).Article 

Google Scholar 
Zobeidi, T., Yaghoubi, J. & Yazdanpanah, M. Developing a paradigm model for the analysis of farmers’ adaptation to water scarcity. Environ. Dev. Sustain. 24, 5400–5425 (2022).Article 

Google Scholar 
Russell, S. & Fielding, K. Water demand management research: A psychological perspective. Water Resour. Res. 46, 1–12 (2010).Article 

Google Scholar 
Shahangian, S. A., Tabesh, M., Yazdanpanah, M., Zobeidi, T. & Raoof, M. A. Promoting the adoption of residential water conservation behaviors as a preventive policy to sustainable urban water management. J. Environ. Manag. 313, 115005 (2022).Article 

Google Scholar 
Onwezen, M. C., Antonides, G. & Bartels, J. The Norm Activation Model: An exploration of the functions of anticipated pride and guilt in pro-environmental behaviour. J. Econ. Psychol. 39, 141–153 (2013).Article 

Google Scholar 
Shahangian, S. A., Tabesh, M. & Yazdanpanah, M. Psychosocial determinants of household adoption of water-efficiency behaviors in Tehran capital, Iran: Application of the social cognitive theory. Urban Clim. 39, 100935 (2021).Article 

Google Scholar 
Yazdanpanah, M., Feyzabad, F. R., Forouzani, M., Mohammadzadeh, S. & Burton, R. J. F. Predicting farmers’ water conservation goals and behavior in Iran: A test of social cognitive theory. Land Use Policy 47, 401–407 (2015).Article 

Google Scholar 
Valizadeh, N., Bijani, M., Hayati, D. & Fallah Haghighi, N. Social-cognitive conceptualization of Iranian farmers’ water conservation behavior. Hydrogeol. J. 27, 1131–1142 (2019).ADS 
Article 

Google Scholar 
Greaves, M., Zibarras, L. D. & Stride, C. Using the theory of planned behavior to explore environmental behavioral intentions in the workplace. J. Environ. Psychol. 34, 109–120 (2013).Article 

Google Scholar 
Wang, Y. et al. Analysis of the environmental behavior of farmers for non-point source pollution control and management: An integration of the theory of planned behavior and the protection motivation theory. J. Environ. Manag. 237, 15–23 (2019).Article 

Google Scholar 
Savari, M. & Gharechaee, H. Application of the extended theory of planned behavior to predict Iranian farmers’ intention for safe use of chemical fertilizers. J. Clean. Prod. 263, 121512 (2020).CAS 
Article 

Google Scholar 
Strydom, W. F. Applying the theory of planned behavior to recycling behavior in South Africa. Recycling 3, 43 (2018).Article 

Google Scholar 
Lam, S. P. Predicting intention to save water: Theory of planned behavior, response efficacy, vulnerability, and perceived efficiency of alternative solutions. J. Appl. Soc. Psychol. 36, 2803–2824 (2006).Article 

Google Scholar 
Abdulkarim, B., Yacob, M. R., Abdullahi, A. M. & Radam, A. Farmers’ perceptions and attitudes toward forest watershed conservation of the North Selangor Peat Swamp Forest. J. Sustain. For. 36, 309–323 (2017).
Google Scholar 
Yuriev, A., Dahmen, M., Paillé, P., Boiral, O. & Guillaumie, L. Pro-environmental behaviors through the lens of the theory of planned behavior: A scoping review. Resour. Conserv. Recycl. 155, 104660 (2020).Article 

Google Scholar 
Bosnjak, M., Ajzen, I. & Schmidt, P. Editorial the theory of planned behavior: Selected recent advances and applications (1841).Ajzen, I. Consumer attitudes and behavior: The theory of planned behavior applied to food consumption decisions. Ital. Rev. Agric. Econ. 70(2), 121–138. https://doi.org/10.13128/REA-18003 (2015).Article 

Google Scholar 
Soorani, F. & Ahmadvand, M. Determinants of consumers’ food management behavior: Applying and extending the theory of planned behavior. Waste Manag. 98, 151–159 (2019).PubMed 
Article 

Google Scholar 
Popa, B., Niță, M. D. & Hălălișan, A. F. Intentions to engage in forest law enforcement in Romania: An application of the theory of planned behavior. For. Policy Econ. 100, 33–43 (2019).Article 

Google Scholar 
Tam, K. P. Understanding the psychology X politics interaction behind environmental activism: The roles of governmental trust, density of environmental NGOs, and democracy. J. Environ. Psychol. 71, 101330 (2020).Article 

Google Scholar 
Ajzen, I. The theory of planned behavior. Organ. Behav. Hum. Decis. Process. 50, 179–211 (1991).Article 

Google Scholar 
Icek, A. From intentions to actions: A theory of planned behavior. in Action Control 11–39 (1985).Empidi, A. V. A. & Emang, D. Understanding public intentions to participate in protection initiatives for forested watershed areas using the theory of planned behavior: A case study of Cameron highlands in Pahang, Malaysia. Sustainability 13, 4399 (2021).Article 

Google Scholar 
Holt, J. R. et al. Using the theory of planned behavior to understand family forest owners’ intended responses to invasive forest insects. Soc. Nat. Resour. 34, 1001–1018 (2021).Article 

Google Scholar 
Marcos, K. J., Moersidik, S. S. & Soesilo, T. E. B. Extended theory of planned behavior on utilizing domestic rainwater harvesting in Bekasi, West Java, Indonesia. IOP Conf. Ser. Earth Environ. Sci. 716, 012054 (2021).Article 

Google Scholar 
Sánchez, M., López-Mosquera, N., Lera-López, F. & Faulin, J. An extended planned behavior model to explain the willingness to pay to reduce noise pollution in road transportation. J. Clean. Prod. 177, 144–154 (2018).Article 

Google Scholar 
Fernandez, M. E., Ruiter, R. A. C., Markham, C. M. & Kok, G. Intervention mapping: Theory-and evidence-based health promotion program planning: Perspective and examples. Front. Public Health 7, 209 (2019).PubMed 
PubMed Central 
Article 

Google Scholar 
Zhong, F. et al. Quantifying the influence path of water conservation awareness on water-saving irrigation behavior based on the theory of planned behavior and structural equation modeling: A case study from Northwest China. Sustainability 11, 1–16 (2019).
Google Scholar 
Ullah, S. et al. Predicting behavioral intention of rural inhabitants toward economic incentive for deforestation in Gilgit-Baltistan, Pakistan. Sustainability 13, 1–17 (2021).
Google Scholar 
Koop, S. H. A., Van Dorssen, A. J. & Brouwer, S. Enhancing domestic water conservation behaviour: A review of empirical studies on influencing tactics. J. Environ. Manag. 247, 867–876 (2019).CAS 
Article 

Google Scholar 
Goh, E., Ritchie, B. & Wang, J. Non-compliance in national parks: An extension of the theory of planned behaviour model with pro-environmental values. Tour. Manag. 59, 123–127 (2017).Article 

Google Scholar 
Liang, Y., Kee, K. F. & Henderson, L. K. Towards an integrated model of strategic environmental communication: Advancing theories of reactance and planned behavior in a water conservation context. J. Appl. Commun. Res. 46, 135–154 (2018).CAS 
Article 

Google Scholar 
Gkargkavouzi, A., Halkos, G. & Matsiori, S. Environmental behavior in a private-sphere context: Integrating theories of planned behavior and value belief norm, self-identity and habit. Resour. Conserv. Recycl. 148, 145–156 (2019).Article 

Google Scholar 
Vaske, J. J., Landon, A. C. & Miller, C. A. Normative influences on farmers’ intentions to practice conservation without compensation. Environ. Manag. 66, 191–201 (2020).Article 

Google Scholar 
Nguru, W. M., Gachene, C. K., Onyango, C. M., Ng’ang’a, S. K. & Girvetz, E. H. Factors constraining the adoption of soil organic carbon enhancing technologies among small-scale farmers in Ethiopia. Heliyon 7, e08497 (2021).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Møller, M., Haustein, S. & Bohlbro, M. S. Adolescents’ associations between travel behaviour and environmental impact: A qualitative study based on the Norm-Activation Model. Travel Behav. Soc. 11, 69–77 (2018).Article 

Google Scholar 
Savari, M., Naghibeiranvand, F. & Asadi, Z. Modeling environmentally responsible behaviors among rural women in the forested regions in Iran. Glob. Ecol. Conserv. 35, e02102 (2022).Article 

Google Scholar 
van Valkengoed, A. M. & Steg, L. Meta-analyses of factors motivating climate change adaptation behaviour. Nat. Clim. Chang. 9, 158–163 (2019).ADS 
Article 

Google Scholar 
Maduku, D. K. Water conservation campaigns in an emerging economy: How effective are they?. Int. J. Advert. 40, 452–472 (2021).Article 

Google Scholar 
Thøgersen, J. & Grønhøj, A. Electricity saving in households—A social cognitive approach. Energy Policy 38, 7732–7743 (2010).Article 

Google Scholar 
Ouellette, J. A. & Wood, W. Habit and intention in everyday life: The multiple processes by which past behavior predicts future behavior. Psychol. Bull. 124, 54–74 (1998).Article 

Google Scholar 
Ajzen, I. The theory of planned behavior: Frequently asked questions. Hum. Behav. Emerg. Technol. 2, 314–324 (2020).Article 

Google Scholar 
Hofmann, W., Gschwendner, T., Friese, M., Wiers, R. W. & Schmitt, M. Working memory capacity and self-regulatory behavior: toward an individual differences perspective on behavior determination by automatic versus controlled processes. J. Pers. Soc. Psychol. 95, 962–977 (2008).PubMed 
Article 

Google Scholar 
Jorgensen, B. S., Martin, J. F., Pearce, M. W. & Willis, E. M. Aligning theory and measurement in behavioral models of water conservation. Water Policy 17, 762–776 (2015).Article 

Google Scholar 
Barr, S. & Gilg, A. W. A conceptual framework for understanding and analyzing attitudes towards environmental behaviour. Geogr. Ann. Ser. B Hum. Geogr. 89 B, 361–379 (2007).Article 

Google Scholar 
Hansmann, R., Bernasconi, P., Smieszek, T., Loukopoulos, P. & Scholz, R. W. Justifications and self-organization as determinants of recycling behavior: The case of used batteries. Resour. Conserv. Recycl. 47, 133–159 (2006).Article 

Google Scholar 
Tang, Z., Chen, X. & Luo, J. Determining socio-psychological drivers for rural household recycling behavior in developing countries: A case study from Wugan, Hunan, China. Environ. Behav. 43, 848–877 (2011).Article 

Google Scholar 
Krejcie, R. V. & Morgan, W. D. (1970) “Determining sample size for research activities”, educational and psychological measurement. Int. J. Employ. Stud. 18, 89–123 (1996).
Google Scholar 
Gregory, G. D. & Di Leo, M. Repeated behavior and environmental psychology: The role of personal involvement and habit formation in explaining water consumption. J. Appl. Soc. Psychol. 33, 1261–1296 (2003).Article 

Google Scholar 
Keramitsoglou, K. M. & Tsagarakis, K. P. Raising effective awareness for domestic water saving: Evidence from an environmental educational programme in Greece. Water Policy 13, 828–844 (2011).Article 

Google Scholar 
Chaudhary, A. K. et al. Using the theory of planned behavior to encourage water conservation among extension clients. J. Agric. Educ. 58, 185–202 (2017).Article 

Google Scholar 
Pradhananga, A. K., Davenport, M. A., Fulton, D. C., Maruyama, G. M. & Current, D. An integrated moral obligation model for landowner conservation norms. Soc. Nat. Resour. 30, 212–227 (2017).Article 

Google Scholar 
Heath, Y. & Gifford, R. Extending the theory of planned behavior: Predicting the use of public transportation. J. Appl. Soc. Psychol. 32, 2154–2189 (2002).Article 

Google Scholar 
Bodimeade, H. et al. Testing the direct, indirect, and interactive roles of referent group injunctive and descriptive norms for sun protection in relation to the theory of planned behavior. J. Appl. Soc. Psychol. 44, 739–750 (2014).Article 

Google Scholar 
Veisi, K., Bijani, M. & Abbasi, E. A human ecological analysis of water conflict in rural areas: Evidence from Iran. Glob. Ecol. Conserv. 23, e01050 (2020).Article 

Google Scholar 
Botetzagias, I., Dima, A. F. & Malesios, C. Extending the Theory of Planned Behavior in the context of recycling: The role of moral norms and of demographic predictors. Resour. Conserv. Recycl. 95, 58–67 (2015).Article 

Google Scholar 
Martínez-Espiñeira, R., García-Valiñas, M. A. & Nauges, C. Households’ pro-environmental habits and investments in water and energy consumption: Determinants and relationships. J. Environ. Manag. 133, 174–183 (2014).Article 

Google Scholar 
Dolnicar, S., Hurlimann, A. & Grün, B. Water conservation behavior in Australia. J. Environ. Manag. 105, 44–52 (2012).Article 

Google Scholar 
Untaru, E. N., Ispas, A., Candrea, A. N., Luca, M. & Epuran, G. Predictors of individuals’ intention to conserve water in a lodging context: The application of an extended Theory of Reasoned Action. Int. J. Hosp. Manag. 59, 50–59 (2016).Article 

Google Scholar 
Khoshmaram, M., Shiri, N., Shinnar, R. S. & Savari, M. Environmental support and entrepreneurial behavior among Iranian farmers: The mediating roles of social and human capital. J. Small Bus. Manag. https://doi.org/10.1111/jsbm.1250158,1064-1088 (2020).Article 

Google Scholar 
Benitez, J., Henseler, J., Castillo, A. & Schuberth, F. How to perform and report an impactful analysis using partial least squares: Guidelines for confirmatory and explanatory IS research. Inf. Manag. 57, 103168 (2020).Article 

Google Scholar 
Sarstedt, M., Ringle, C. M. & Hair, J. F. Partial least squares structural equation modeling. in Handbook of Market Research 1–47. https://doi.org/10.1007/978-3-319-05542-8_15-2 (2021).Clark, W. A. & Finley, J. C. Determinants of water conservation intention in Blagoevgrad, Bulgaria. Soc. Nat. Resour. 20, 613–627 (2007).Article 

Google Scholar 
De Dominicis, S., Sokoloski, R., Jaeger, C. M. & Schultz, P. W. Making the smart meter social promotes long-term energy conservation. Palgrave Commun. 5, 1–8 (2019).Article 

Google Scholar 
Wang, S., Hung, K. & Huang, W.-J. Motivations for entrepreneurship in the tourism and hospitality sector: A social cognitive theory perspective. Int. J. Hosp. Manag. https://doi.org/10.1016/j.ijhm.2018.11.018 (2018).Article 

Google Scholar 
Ramirez, E., Kulinna, P. H. & Cothran, D. Constructs of physical activity behaviour in children: The usefulness of Social Cognitive Theory. Psychol. Sport Exerc. 13, 303–310 (2012).Article 

Google Scholar 
Glanz, K., Rimer, B. K. & Viswanath, K. Health and Health (2002). More