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    Environmental changes associated with drying climate are expected to affect functional groups of pro- and microeukaryotes differently in temporary saline waters

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    Enzyme adaptation to habitat thermal legacy shapes the thermal plasticity of marine microbiomes

    Extraction of total active proteomes from sediment samplesWe sampled 14 sediments along the coastlines of the Irish Sea, the Mediterranean Sea, and the Red Sea (from 16°N to 53°N), applying uniform sampling and storage procedures. Location details and sediment temperature fluctuations are summarized in Supplementary Table S1. We collected sediments (5 Kg) in triplicate and extracted the total proteins using a well-established microbial detachment procedure67, with some modifications. We mixed 100 g of sediment with 300 ml of sterilized saline solution (5 mM sodium pyrophosphate and 35 g L−1 of NaCl) containing 150 mg L−1 of Tween 80 (from Merck Life Science S.L.U., Madrid, Spain) in an ice water bath. After re-suspension, samples were kept in a water bath ultra-sonicator (Bandelin SONOREX, Berlin, Germany) on ice and sonicated (60 W output) for 120 min. We repeated this procedure twice, with an ice water bath incubation of 60 min between each cycle. We then centrifuged the samples at 500 g for 15 min at 4 °C to remove the sediments in a centrifuge 5810 R (Eppendorf AG, Hamburg, Germany). Supernatants were carefully transferred to a new tube, minimizing disruption of the sediments, and the resulting supernatants were centrifuged at 13,000 g for 15 min at 4 °C to produce microbial cell pellets. We used the resulting cell mix to extract the total protein by mixing the cells with 1.2 ml BugBuster® Protein Extraction Reagent (Novagen, Darmstadt, Germany) for 30 min with shaking (250 rpm). Subsequently, samples were disrupted by sonication using a pin Sonicator® 3000 (Misonix, New Highway Farmingdale, NY, USA) for a total time of 2 min (10 watts) on ice (4 cycles × 0.5 min with 1 min ice-cooling between each cycle). Extracts were centrifuged for 10 min at 12,000 g at 4 °C to separate cellular debris and intact cells. Supernatants were carefully aspirated (to avoid disturbing the pellet), transferred to new tubes, and stored at –80 °C until use. The protein solution was filtered at 15 °C for 7 h using Vivaspin filters (Sartorius, Goettingen, Germany) with a molecular weight (MW) cut-off of 3,000 Da to concentrate the proteins up to a final concentration of 10 mg ml−1, according to the Bradford Protein Assay (Bio-Rad Laboratories, S.A., Madrid, Spain)68. The average total amount of proteins extracted per each 100 g of sediment was 612 µg (interquartile range, 31 µg, see details in Supplementary Fig. S2). In all cases, extensive dialysis of protein solutions against 40 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer was performed using a Pur-A-LyzerTM Maxi 1200 dialysis kit (Merck Life Science S.L.U., Madrid, Spain)69, and active proteins stored at a concentration of 10 mg ml−1at –86 °C until use. As reported previously70, 2DE was performed using GE Healthcare reagents and equipment, 11 cm IPG strips in the pH range of 3–10 and molecular weight ranging from 10 to 250 kDa (Precision Plus Protein Dual Color Standards #1610374, Bio-Rad Laboratories, S.A., Madrid, Spain). The 2-DE was performed using a validated pooling strategy71, in which proteins extracted from three independent biological replicates (i.e., sediments) were mixed in equal amounts and a total of 150 µg of protein were further loaded per gel. Staining was performed with SYPRO Ruby Protein Gel Stain (Invitrogen, Waltham, MA, USA). The two-dimensional SDS-PAGE (12% acrylamide) gels of extracted proteins are reported in Supplementary Fig. S2 (original gels in Source Data). The same protocol was applied to extract and analyse by SDS-PAGE the total active proteins extracted from sediment samples with different temperature variability levels (HTV, ITV, and LTV) collected in the Red Sea (Supplementary Table S4). The total amount of protein extracted per each 100 g of sediment is given in Supplementary Table S8. Coomassie-stained one-dimension SDS-PAGE (1-DE) gels of extracted proteins are shown in Supplementary Fig. S9 (original gel in Source Data).Source, expression and purification of esterases and EXDOs from a wide geographical rangeWe recovered 83 enzymes (78 esterases and 5 EXDO) from microbial communities inhabiting marine sediments across ten distinct locations from the latitudinal transect described above: Ancona harbour (Anc), Priolo Gargallo (Pri), Gulf of Genoa, Messina harbour (Mes), Milazo harbour (Mil), Mar Chica lagoon (MCh), Bizerte lagoon (Biz), El-Max site (ElMax), Gulf of Aqaba (Aq), and Menai Strait (MS); further details are provided in Supplementary Data S3. Sources of the enzymes were the corresponding shotgun metagenomes (see Supplementary Table S3) and the metagenome clone libraries generated from the extracted DNA71. The sediment sample from the Gulf of Genoa was not used for activity tests and metaproteome analysis because no raw sample material was available; however, because of the possibility to access its shotgun metagenome (see Supplementary Table S3) and a metagenome clone library72, we used the sample for screening esterases to incorporate an additional latitude in our transect. In the case of Menai Strait (Irish Sea), five additional esterases were retrieved from a metagenome obtained from enriched cultures prepared with samples collected on 22nd June 2019 from Menai Strait (School of Ocean Sciences, Bangor University, St. George’s Pier, Menai Bridge, N53°13′31.3″; W4°09′33.3”). The water temperature was 14 °C and the salinity was 32 p.s.u. Two enrichment cultures were set up at 20 °C: (i) SW: seawater enrichment with 0.1% lignin; the enrichment was set up using 50 ml of the sample as inoculum with the addition of 0.1% lignin (Sigma-Aldrich, Gillingham, United Kingdom) (w/v); (ii) AW: algal surface wash-off in seawater, enriched with 0.1% lignin; the enrichment was set up using 50 ml of surface wash-off after mixing of ca. 10 g of Fucus (brown algae) in the seawater and removal of plant tissue, 0.1% lignin (w/v) was added. After 92 days of incubation, 5 ml of each enrichment cultures were transferred into the new flask containing 45 ml autoclaved and filtered seawater with 0.1% lignin. This procedure was repeated on days 185 and 260, and the incubation was stopped on day 365. The DNA was extracted using 12 months using MetaGnome extraction kit (EpiCentre, Biotechnologies, Madison, WI, USA), sequenced on Illumina MiSeq™ platform (Illumina Inc., San Diego, CA, USA) using paired-end 250 bp reads at the Centre for Environmental Biotechnology (Bangor, UK), and sequencing reads were processed and analysed as described previously73.The screening, cloning and activity of a subset of 35 identified esterases have been reported previously72. The remaining 48 enzymes are reported for the first time in this study and were identified using naive and in silico metagenomic approaches, as detailed below. The environmental site from which each enzyme originated and the method employed for its identification are detailed in Supplementary Data S3. For naive screens addressing the recovery of new sequences encoding esterases and EXDO, the large-insert pCCFOS1 fosmid libraries made using the corresponding DNA samples, the CopyControl Fosmid Library Kit (Epicentre Biotechnologies, Madison, WI, USA) and the Escherichia coli EPI300-T1R strain were used. The nucleic acid extraction, construction and the functional screens of such libraries have been previously described72. In brief, fosmid clones were plated onto large (22.5 × 22.5 cm) Petri plates with Luria Bertani (LB) agar containing chloramphenicol (12.5 µg ml−1) and induction solution (Epicentre Biotechnologies; WI, USA), at a quantity recommended by the supplier to induce a high fosmid copy number. Clones were scored by the ability to hydrolyze α-naphthyl acetate and tributyrin (for esterase activity), and catechol (for EXDO activity)72,74. Positive clones presumed to contain esterases and EXDOs were selected, and their DNA inserts were sequenced using a MiSeq Sequencing System (Illumina, San Diego, USA) with a 2 × 150-bp sequencing v2 kit at Lifesequencing S.L. (Valencia, Spain). After sequencing, the reads were quality-filtered and assembled to generate nonredundant meta-sequences, and genes were predicted and annotated via BLASTP and the PSI-BLAST tool72. For in silico screens, addressing the recovery of new sequences encoding esterases, the predicted protein-coding genes, obtained after the sequencing of DNA material from resident microbial communities in each of the samples, were used. The meta-sequences are available from the National Center for Biotechnology Information (NCBI) nonredundant public database (accession numbers reported in Supplementary Data S3). Protein-coding genes identified from the DNA inserts of positive clones (naive screen) or from the meta-sequences were screened for enzymes of interest using the Blastp algorithm via the DIAMOND v2.0.9 program with default parameters (percentage of identity ≥60%; alignment length ≥70; e-value ≤1e−5)29, against the Lipase Engineering sequence databases (to screen for esterases) and AromaDeg database (for EXDO)74. Since the collection of sediments across locations experiencing different MATs was limited by our sampling capacity, to expand our range of exploration at a global scale and to validate our dataset, we added our single enzyme analysis to the seawater metagenomes retrieved from the Tara Ocean Expedition database (accession number in Supplementary Data S4). Due to the volume of sequences generated, this database provides access to a large number of enzymes, including those studied here through homology search. Esterases were selected as target sequences, and the following pipeline was used. First, we selected a sequence encoding an esterase reported as one of the most substrate-ambiguous esterases out of 145 tested (EH1, Protein Data Bank acc. nr. 5JD4) and well-distributed in the marine environment72. Second, we performed a homology search of this sequence against the Tara Ocean metagenome21 to retrieve similar sequences, using the Blastp algorithm via the DIAMOND v2.0.9 program30 (e-value 98% using SDS-PAGE analysis in a Mini PROTEAN electrophoresis system (Bio-Rad Laboratories, S.A., Madrid, Spain). Purified protein was stored at –86 °C until use at a concentration of 10 mg ml−1 in 40 mM HEPES buffer (pH 7.0). A total of approximately 5–40 mg of total purified recombinant protein was obtained from 1 L of culture. Supplementary Fig. S1 illustrates a schematic representation of the pipeline implemented in this work to investigate enzyme activities in a large set of marine samples, starting from samples collected (sediments) and available metagenomes.Enzyme activity assessmentsAll substrates used for activity tests were of the highest purity and, if not indicated otherwise, were obtained from Merck Life Science S.L.U. (Madrid, Spain): 4-nitrophenyl-propionate (ref. MFCD00024664), 4-nitrophenyl phosphate (ref. 487663), 4-nitrophenyl β-D-galactose (ref. N1252), bis(p-nitrophenyl) phosphate (ref. 123943), benzaldehyde (ref. B1334), 2-(4-nitrophenyl)ethan-1-amine (ref. 184802-5G), pyridoxal phosphate (ref. P9255), acetophenone (ref. A10701), NADPH (ref. N5130) and catechol (ref. PHL82372). We directly tested total protein extracts for esterase, phosphatase, beta-galactosidase, and nuclease activity using 4-nitrophenyl-propionate, 4-nitrophenyl phosphate, 4-nitrophenyl β-D-galactose, and bis(p-nitrophenyl) phosphate, respectively, by following the production of 4-nitrophenol at 348 nm (extinction coefficient [ε], 4147 M−1 cm−1), as previously described69. For determination: [total protein]: 5 μg ml−1; [substrate]: 0.8 mM; reaction volume: 200 μl; T: 4–85 °C; and pH: 8.0 (50 mM Tris-HCl buffer). The hydrolysis of 4-nitrophenyl-propionate was used to determine, under these standard conditions, the effects of temperature on the purified esterase. Transaminase activity was determined using benzaldehyde as amine acceptor, 2-(4-nitrophenyl)ethan-1-amine as amine donor, and pyridoxal phosphate as a cofactor, by following the production of a colour amine at 600 nm (extinction coefficient, 537 M−1 cm−1), as previously described75. For determination, [total protein]: 5 μg ml−1; [substrates]: 25 mM; [pyridoxal phosphate]: 1 mM; reaction volume: 200 μL; T: 4-85 °C; and pH: 8.0 (50 mM Tris-HCl buffer). Aldo-keto reductase activity was determined using acetophenone as a substrate and NADPH as a cofactor, by following the consumption of NADPH at 340 nm (extinction coefficient, 6220 M−1 cm−1), as described76. For determination, [total protein]: 5 μg ml−1; [substrate]: 1 mM; [cofactor]: 1 mM; reaction volume: 200 μL; T: 4–85 °C; and pH: 8.0 (50 mM Tris-HCl buffer). We determined EXDO activity using catechol as substrate, by following the increase of absorbance at 375 nm of the ring fission products (extinction coefficient, 36000 M−1 cm−1), as previously described74. For determination, [protein]: 5 μg ml−1; [catechol]: 0.5 mM; reaction volume: 200 μL; T: 4–85 °C; and pH: 8.0 (50 mM Tris-HCl buffer). The hydrolysis of catechol was used to determine, under these standard conditions, the effects of temperature on the purified EXDOs. All measurements were performed in 96-well plates (ref. 655801, Greiner Bio-One GmbH, Kremsmünster, Austria), in biological triplicates over 180 min in a Synergy HT Multi-Mode Microplate Reader (Biotek Instruments, Winooski, VT, USA) in continuous mode (measurements every 30 s) and determining the absorbance per minute from the slopes generated and applying the formula (1). All values were corrected for nonenzymatic transformation.$${Rate}left(frac{mu {mol}}{{{min }}{mg},{protein}}right)= frac{frac{triangle {{{{{rm{Abs}}}}}}}{{{min }}}}{{{{{{rm{varepsilon }}}}}},{{{{{rm{M}}}}}}-1{{{{{rm{cm}}}}}}-1}*frac{1}{0.4,{cm}}*frac{{10}^{6},mu M}{1{{{{{rm{M}}}}}}}\ *0.0002,L*frac{1}{{mg},{protein}}$$
    (1)
    Shotgun proteomicsProteomics was performed by using total active proteins (extracted as above), which were then subjected to protein precipitation, protein digestion and Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometric (LC-ESI-MS/MS) analysis, as previously described77. High-quality reference metagenomes corresponding to each sample (BioProject number in Supplementary Table S3) were used for protein calling, with a threshold of only one identified peptide per protein identification because False Discovery Rates (FDR) controlled experiments counter-intuitively suffer from the two-peptide rule. The confidence interval for protein identification was set to ≥95% (p  50 °C for which the second phase transition was chosen to focus on the decomposition of the core. It is important to note that applying CNA to MD simulations at room temperature may lead to an evening out of Tp values for esterases that transition around this temperature, i.e., systems with a Tp at or below room temperature might all be influenced similarly by loosening their bonding network. By contrast, systems with a transition temperature at or above room temperature would still be discriminated against. The data generated in this study for analyzing Tp values have been deposited at researchdata.hhu.de under accession code DOI: 10.25838/d5p-42101 [https://doi.org/10.25838/d5p-42].Relationship of temperature-induced changes in enzymeRelationship between MAT and enzyme response to temperature (i.e., Topt, Td and Tp) were evaluated by performing linear regression in R. In the case of enzymes retrieved from the Tara ocean dataset we calculated first the break point (flexus) using the package segmented in R102 and then we computed separately the linear model describing the two linear regressions before and after the breakpoint. To evaluate the possible relation between enzyme thermal response and other environmental parameters, salinity and pH data were retrieved from Bio-ORACLE52 using GPS coordinates of each location.Environmental characterization and sediment collection from different temperature variability levels in the Red SeaWe recorded the temperatures of surface sediments from March 2015 to September 2016 along the coast of the Red Sea using HOBO data loggers (Onset, USA) in nine stations located at 3, 25, and 50 m depth. Details on the location, depth and temperature fluctuations of the studied sediments are reported in Supplementary Table S4 and Source Data. We first assess the differences in the homogeneity of the temperature variance in the three types of sediments to evaluate the magnitude of thermal variation and then we test the difference among their MATs using a non-parametric ANOVA (Dunnett’s multiple comparisons tests). We identified three different levels of temperature variability (Fig. 3a–c; Supplementary Table S5): high, intermediate, and low thermal variability (HTV, ITV, and LTV, respectively), where sediments experienced temperature variations of 12.8 °C, 8.8 °C, and 6.7 °C, respectively. From each station, we sampled 200 g of surface sediment (0–5 cm depth) in triplicate in August and December 2015 with a Van der Venn grab (1 dm3) equipped with a MicroCat 250 Seabird CTD (Conductivity, Temperature, Depth), which was assembled on board the research vessel R/V Explorer (KAUST). During sampling, we measured the temperature of the sediments and the water layer covering the sediments using a digital thermometer and the CTD, respectively. We conducted all sampling in compliance with the guidelines specified by KAUST and Saudi Arabian authorities.Sediment processing for analysis of bacterial communitiesFrom each sample (in triplicate), we immediately removed subsamples of sediment (n = 54, ~10 g) and stored them at –20 °C for molecular analysis. Separately, sediment 25 ± 1 g was transferred to 50 ml tubes and added 30 ml of filtered (0.2 µm) water from the Red Sea. The tubes were shaken at 500 rpm for one hour and then centrifuged them at 300 g for 15 min to detach the microbial cells in the sediments without affecting their vitality103,104. The supernatant containing the extracted cells was collected in sterile tubes and was immediately used to measure microbial growth rates.Evaluation of bacterial growth in sediments at different temperaturesWe evaluated the microbial growth rate of the heterotrophic community extracted from the sediments under HTV, ITV, and LTV at 10 °C, 20 °C, 30 °C, 40 °C and 50 °C, using Marine Broth as the cultivation medium (Zobell Marine Broth 2216) supplemented with 0.1 g/L cycloheximide; a rich-medium was selected to avoid the nutrient limitation effect that can affect bacterial physiology63,105. We inoculated 96-well plates with 200 µl of cultivation medium and 25 µl of the cell suspension extracted from the sediments. We inoculated the three biological replicates from each station and each level of temperature variability in eight wells, giving a total of 72 wells for each plate, with 24 wells used as a negative control inoculated with water. We assembled a total of three plates for each incubation temperature from August and December. Plates were spectrophotometrically measured at 3 h intervals using an optical density of 600 nm (Spectramax® M5) for 72 h. Wells with optical density 90%) for further analysis (Supplementary Tables S9 and S10). We calculated the compositional similarity matrix (Bray-Curtis of the log-transformed OTU table) with Primer 6109. Using the same software, canonical analysis of principal coordinates (CAP)110 was used to compare the temperature variability samples (temperature variability levels: HTV, ITV, and LTV; season levels: August and December) based on the compositional similarity matrix. We applied permutational multivariate analyses of variance to the matrix (PERMANOVA; main and multiple comparison tests). We tested the occurrence of thermal-decay patterns in sediments with different temperature variability levels using linear regression (Prism 9.2 software, La Jolla California USA, www.graphpad.com) between the bacterial community similarities (Bray-Curtis) and the temperature differences among sediments (∆T°C) at the time of sampling. We calculated alphadiversity indices (richness and evenness) using the paleontological statistics (PAST) software, and their correlation with temperature was modelled using linear regression in Prism 9.2. Spearman correlation among temperature and relative abundance of OTUs within each sediment sample was evaluated; OTUs were classified based on their positive (enriched) and negative (depleted) correlation with sediment temperature.Reporting summaryFurther information on research design is available in the Nature Portfolio Reporting Summary linked to this article. More

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    Two wild carnivores selectively forage for prey but not amino acids

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    Genetic diversity of virus auxiliary metabolism genes associated with phosphorus metabolism in Napahai plateau wetland

    Screening for viral AMGsViral protein annotation using VIBRANT and DRAM-v software combined with manual proofreading identified the viral AMGs in Napahai plateau wetland, including the viral AMGs phoH, phoU and pstS, which were associated with phosphorus metabolism.Phylogenetic analysis of AMGs associated with phosphorus metabolism in Napahai plateau wetlandThere were 24 amino acid sequences of phoH gene in Napahai plateau wetland (Fig. 1A). They were divided into 5 clusters, the largest of which had 10 sequences, while the smallest cluster had only 1 sequence. The remaining 3 clusters contained 6, 5 and 2 sequences, respectively. The phoH gene was genetically diverse in Napahai plateau wetland, which might be related to the different host origins. A total of 74 sequences of phoU gene could be found in seven clusters (Fig. 1B), with the largest cluster containing 27 sequences and the smallest cluster having two sequences. Similar to phoH, phoU was also genetically diverse, but richer than that of phoH. There were 71 pstS sequences forming 9 clusters, with the largest cluster of 23 sequences and the smallest cluster only 1 sequence (Fig. 1C). It could be seen that the genetic diversity of pstS was better than that of phoH and phoU, which might be related to the unique geographical location. Napahai plateau wetland is located in the Three Parallel Rivers of Yunnan protected areas, which forms a complex landscape, and then controls the evolution and characteristics of organisms, thus showing abundant biodiversity. Li et al. obtained 58 phoH gene sequences from Northeastern wetland sediments of China, which were 22%–99% consistent at the amino acid level, and found that the phoH gene could regulate phosphate uptake and metabolism under the low phosphate or phosphate limitation conditions16. However, the exact function remained unclear. The phoH gene clustered into five clusters in Napahai plateau wetland, indicating high genetic diversity. Additionally, water and soil samples were collected from eight separate sampling sites, and there were differences between samples environments, which might also have an impact on the genetic diversity of the three genes.Figure 1Phylogenetic analysis of phosphorus metabolism AMGs in Napahai plateau wetland, different colors represent different branches. (A) Phylogenetic analysis of phoH genes. (B) Phylogenetic analysis of phoU genes. (C) Phylogenetic analysis of pstS genes.Full size imagePhylogenetic analysis and PCoA analysis of AMGs associated with phosphorus metabolism from different habitats and host originsIn order to understand the genetic diversity of viral AMGs (phoH, phoU, pstS) associated with phosphorus metabolism in Napahai plateau wetland, a phylogenetic tree of phosphorus metabolism AMGs from different habitats was constructed, and PCoA analysis was performed (Fig. 2). The results showed that most sequences of phoH, phoU and pstS genes in Napahai plateau wetland clustered individually, especially phoU and pstS genes, and only a few sequences were closely related to those of other habitats. In Fig. 2A, 14 sequences clustered individually and were relatively far from sequences of other habitats, whereas 7 sequences were close to those from freshwater lakes, and other 3 sequences were close to those from rice fields, oceans and other wetlands, respectively. Therefore, the genetic diversity of phoH in Napahai plateau wetland was independent of the habitat. Moreover, some of the phoH sequences were clustered with those of other habitats and distributed in the fourth quadrants (Fig. 2D). From Fig. 2B, apart from 3 sequences which clustered with those from the marine habitats and freshwater lakes, the rest were clustered separately. Whereas in Fig. 2E, apart from only a few sequences, most sequences of phoU were far away from those of different habitats, which was consistent with Fig. 2B. Thus, the genetic diversity of phoU gene in Napahai wetland was also independent of habitat, where the separately clustered sequences may be unique. From Fig. 2C, we can seen that apart from 8 sequences which more closely related to those from the freshwater lake, ocean, rice field, and other wetlands, all the rest were individually clustered. The result was consistent with that of Fig. 2F. Therefore, the genetic diversity of the pstS gene was also habitat-independent.Figure 2Phylogenetic analysis and PCoA of phosphorus metabolism AMGs in different habitats, different colors represent different habitats. (A) Phylogenetic analysis of phoH genes in different habitats. (B) Phylogenetic analysis of phoU genes in different habitats. (C) Phylogenetic analysis of pstS genes in different habitats. (D) PCoA analysis of phoH genes in different habitats. (E) PCoA analysis of phoU genes in different habitats. (F) PCoA analysis of pstS genes in different habitats.Full size imageTo study whether the genetic diversity was related to host origins, three AMGs associated with phosphorus metabolism were selected for phylogenetic and PCoA analyses with AMGs sequences from different host origins (Fig. 3). It showed that some sequences of all three genes were similar to those from different host origins, while the remaining were separately clustered. In Fig. 3A, apart from 14 sequences which clustered with those from fungi, bacteria, non-culturable phages, phages and viruses, all the rest were clustered separately. Whereas, most sequences were clustered with those from different host origins together, and only six sequences were far from other sequences of different host origins based on PCoA analysis (Fig. 3D). Only three sequences were clustered with those of archaea and uncultured archaea, and the rest were clustered together to form independent clusters (Fig. 3B). A small amount of sequences were gathered with bacteria, uncultured bacteria, archaea and uncultured archaea, and the rest were clustered individually (Fig. 3E). As can be seen in Fig. 3C, six sequences were clustered with those of archaea, fungi, bacteria, while the rest were clustered separately. Some sequences were gathered with bacteria, uncultured bacteria, archaea and uncultured archaea, and others were clustered separately (Fig. 3F). PCoA analysis was largely consistent with phylogenetic analysis. So the genetic diversity of phoH, phoU and pstS genes in Napahai plateau wetland was independent of the host origins.Figure 3Phylogenetic analysis and PCoA of phosphorus metabolism AMGs from different host origins, different colors represent different host origins. (A) Phylogenetic analysis of phoH gene from different host origins. (B) Phylogenetic analysis of phoU gene from different host origins. (C) Phylogenetic analysis of pstS gene from different host origins. (D) PCoA analysis of phoH genes from different host origins. (E) PCoA analysis of phoU genes from different host origins. (F) PCoA analysis of pstS genes from different host origins.Full size imageOverall, the genetic diversity of phoH, phoU and pstS genes associated with phosphorus metabolism in Napahai plateau wetland was independent of both the habitats and host origins based on phylogenetic and PCoA analyses. It suggested that three genes showed relatively rich genetic diversity and were not genetically limited by differences in habitats or host origins. Han et al. showed that phoH sequences were widely distributed in soil, freshwater, and seawater environments in different locations around the world, indicating the genetic diversity independent of the environment17, which corroborated the conclusions in our study. Phylogenetic analysis of the 58 viral phoH gene sequences in Northeastern wetland of China revealed that some sequences were clustered with bacterial sequences and others clustered with phages sequences16. In Napahai plateau wetland, some phoH gene sequences were clustered with fungal, bacterial, phage, uncultured phage, and viruses. Hence, the genetic diversity of phoH gene was independent of the host origins in either Northeastern wetland or Napahai plateau wetland. Compared with Northeastern wetland, the phoH genes in Napahai plateau wetland showed more abundant genetic diversity, which may be related to geographical location and climate. Additionally, compared with sequences from different habitats and host sources, partial sequences from Napahai plateau wetland were clustered individually, thus they were unique, which might be related to the unique geography. Napahai plateau wetland is located in the Three Parallel Rivers with low latitude and high altitude, and shows specific characteristics which not found in other habitats, and then the species very different, thus providing the possibility for the emergence of unique genetic sequences. Of course, it would require further verification by subsequent study.As far as the current studies are concerned, most reports on phosphorus AMGs focused on the function. Wang et al. mentioned that the phoH gene regulated phosphate uptake or metabolism under the low phosphorus or phosphate limitation conditions18. Kelly et al. isolated several phages from oligotrophic water bodies with low phosphorus condition, found that they contained the phosphate binding transporter gene pstS by sequencing, which enhanced the host cell with increasing the infection cycle of phages by increasing phosphate utilization19. Gardner et al. studied the PhoR-PhoB two-component regulatory system in E. coli, which regulated the expression of relevant genes according to environmental phosphate concentration and enabled cells to adapt the phosphate starvation20. The phoU existed in many bacteria and was identified as an auxiliary protein of the phosphate-specific transporter system, regulating phosphate metabolism in the host cell acting as phosphate regulators21. Few studies had been conducted on its genetic diversity, therefore, the information on the genetic diversity was relatively scarce.α diversity analysis of phosphorus metabolism AMGs in different habitats and different host originsChao, Shannon and Simpson diversity indices are common mathematical measure of species alpha diversity in the community. Chao focuses on species richness. Shannon index and Simpson index measure species richness and evenness. Simpson reinforces evenness and Shannon reinforces richness22.Sequences from different habitats, such as Napahai plateau wetland, Pacific Ocean, Lake Baikal, Northeast rice fields, glaciers, and wetlands, were selected for α-diversity analysis (Fig. 4). The genetic diversity indices, such as Chao, Shannon and Simpson, calculated based on the OUT dataset, were used to characterize the alpha diversity. Among them, larger Chao values, smaller Simpson values or larger Shannon values indicate higher genetic diversity. Only at the level of Chao values (Fig. 4A,D,G) and Shannon values (Fig. 4B,E,H), the values of phoH, phoU, and pstS in Napahai plateau wetland were greater than those from other habitats, indicating better heritable, which might be related to the unique geographical location and abundant water resources. The geographical location made it unique and less influenced by external factors, and abundant water resources created a rich biodiversity, thus providing a good genetic environment. From the Simpson values (Fig. 4C,F,I), the values of phoU and pstS genes were smaller than those of other habitats, indicating better inherited. For the phoH gene, the Simpson value was closer in magnitude and lower than those in Antarctic Lake and wetlands, indicating better heritable.Figure 4Plots of genetic diversity indices analysis of phosphorus metabolism AMGs in different habitats, different colors represent different genetic diversity indices. (A, D, G) Represent respectively the Chao values of phoH, phoU, and pstS genes in different habitats. (B, E, H) Represent respectively the Shannon values of phoH, phoU, and pstS genes in different habitats. (C, F, I) Represent respectively the Simpson values of phoH, phoU, and pstS genes in different habitats.Full size imageThree AMGs associated with phosphorus metabolism in Napahai plateau wetland were selected for α-diversity analysis with AMGs sequences from different host origins (Fig. 5). In Fig. 5A, the Chao values of phoH gene from bacteria, phages, uncultured phages and uncultured viruses in Napahai plateau wetland were smaller than those of bacteria, phages, uncultured phages and uncultured viruses, indicating the poor genetic diversity. In addition, compared to the genetic diversity of sequences from other host sources, the genetic diversity of phoH gene from bacteria in Napahai plateau wetland was better. As can be seen in Fig. 5D, G, the Chao values of phoU and pstS genes from bacteria in Napahai plateau wetland were greater than those of other host origins, indicating better genetic diversity, while the Chao values of pstS genes from archaea in Napahai plateau wetland were smaller than those of other host origins, indicating poor genetic diversity.Figure 5Plots of genetic diversity indices analysis of phosphorus metabolism AMGs from different host origins, different colors represent different genetic diversity indices. (A, D, G) Represent respectively the Chao values of phoH, phoU, and pstS genes from different host origins. (B, E, H) Represent respectively the Shannon values of phoH, phoU, and pstS genes from different host origins. (C, F, I) Represent respectively the Simpson values of phoH, phoU, and pstS genes from different host origins.Full size imageThe Shannon value of phoH gene from bacteria in Napahai plateau wetland was smaller than that of bacteria and uncultured viruses, indicating poor diversity, but larger than other host sources, indicating better genetic diversity (Fig. 5B). The Shannon values of phoH gene from phages and uncultured phages in Napahai plateau wetland were lower than those of other host origins, indicating poor diversity. The Shannon value of phoH genes from uncultured viruses in Napahai plateau wetland was 0, probably due to sample size too small to calculate the Shannon value. In Fig. 5E, H, the Chao values of phoU and pstS genes from bacteria in Napahai plateaus wetland were greater than those from other host sources, indicating better diversity, while the Shannon value of pstS gene from archaea in the Napahai plateau wetland was 0, probably small sample size.The Simpson values of phoH genes from phage, uncultured phage and uncultured virus in Napahai plateau wetland were smaller than those of other host origins (except uncultured virus), indicating better diversity. The smaller Simpson values of phoH genes related to fungi, phages, uncultured phages, and viruses indicated better diversity, while the larger Simpson values compared to bacteria, phages, and uncultured viruses indicated poor diversity (Fig. 5C). As can be seen in Figs. 5F,I, the Simpson values of phoU genes from bacteria and pstS genes from bacteria and archaea in Napahai plateau wetland were smaller than those of other host origins, indicating better genetic diversity.Currently, most studies on phosphorus AMGs employed phylogenetic analysis16,23. In contrast, relatively few AMGs associated with phosphorus had been reported based on α-diversity analysis, so it was difficult to obtain specific values of α-diversity indices in other studies.Biogeochemical cycling of AMGs associated with phosphorus metabolism in Napahai plateau wetlandViruses are the gene carriers in susceptible hosts, and AMGs introduced by viruses into new hosts can enhance viral replication and/or influence key microbial metabolic pathways of the biogeochemical cycles24. It is well known that phosphorus is an essential nutrient and plays essential roles in cells25. Phosphorus deficiency leads to restricted cell division, down-regulation of photosynthesis, reduced protein and nitrogen content and chlorophyll synthesis26. To study the effect of AMGs associated with phosphorus metabolism, a phosphorus metabolic pathway containing phoH, phoU and pstS genes was constructed based on metagenomic data (Fig. 6). When phosphorus deficiency occurs in the host, it leads to the expression of phoH, phoU and pstS genes. phoH is a phosphate starvation inducible gene, while pstS acts as a phosphate transport gene and phoU belongs to a phosphate regulatory gene that produces dissolved inorganic phosphorus (DIPs), which then undergoes a series of reactions to produce ATP. The generated ATP becomes PolyP under the action of ppK which encoding polyphosphate kinase, or is used in Calvin cycle to provide energy for Ru5P to produce RuBP, or is used for DNA biosynthesis to provide energy. PolyP is regenerate into DIP with ppX which encoding exopolyphosphatase, and also involves in the biosynthesis process of DNA as Pi to provide phosphate for the nucleic acids synthesis. Thus, phosphorus metabolism of AMGs invoved plays a significant role in the life process of the virus and host. In addition, phoE and ugpQ genes also are identified in Napahai plateau wetland, but their roles in the phosphorus cycling are currently unknown and need further study.Figure 6Biogeochemical cycling of AMGs associated with phosphorus metabolism in Napahai plateau wetland. Red line indicates the process of phosphorus metabolism.Full size imageBased on the phylogenetic and PCoA analyses, we found that the phoH, phoU, and pstS genes all showed unique sequences, which might be drive the microorganisms to produce the phosphorus metabolic pathway in Napahai plateau wetland. Of course, in order to prove this pathway, further validation might be done by metabolomics or metabolic flow method. Furthermore, the phosphorus metabolic pathway was poorly reported, so we could not compare with the phosphorus pathway from other environment to find commonalities and differences. More

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