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Russia invades UkraineThe global science community was quick to condemn Russian’s invasion of Ukraine in February. Research organizations moved fast to cut ties with Russia, stopping funding and collaborations, and journals came under pressure to boycott Russian authors.The situation escalated when Russian forces attacked Europe’s largest nuclear power plant, Zaporizhzhia, in March, prompting fears of a nuclear accident. Russian troops continue to occupy the power plant. Since the invasion began, thousands of civilians have been killed and millions displaced; many others, including scientists, have fled the country.The war has affected research in space and climate science, disrupted fieldwork and played a significant part in the global energy crisis. The invasion could also precipitate a new era for European defence research.JWST delights astronomers

Stephans Quintet, a grouping of five galaxies, taken by NASA’s James Webb Space Telescope.Credit: NASA, ESA, CSA, and STScI via Getty

NASA’s James Webb Space Telescope (JWST) — the most complex telescope ever built — reached its destination in space in January after decades of planning. In July, astronomers were awed by the telescope’s first image — of thousands of distant galaxies in the constellation Volans. Since then, the US$10-billion observatory has captured a steady stream of spectacular images, and astronomers have been working feverishly on early data. Insights include detailed observations of an exoplanet, and leading contenders for the most distant galaxy ever seen.NASA also decided not to rename the telescope, despite calls from some astronomers to do so because the telescope’s namesake, a former NASA administrator, held high-ranking government positions in the 1950s and 1960s, when the United States systematically fired gay and lesbian government employees. A NASA investigation “found no evidence that Webb was either a leader or proponent of firing government employees for their sexual orientation”, the agency said in a statement in November.AI predicts protein structuresResearchers announced in July that they had used the revolutionary artificial-intelligence (AI) network AlphaFold to predict the structures of more than 200 million proteins from roughly one million species, covering almost every known protein from all organisms whose genomes are held in databases. The development of AlphaFold netted its creators at the London-based AI company DeepMind, owned by Alphabet, one of this year’s US$3-million Breakthrough prizes — the most lucrative awards in science.AlphaFold isn’t the only player on the scene. Meta (formerly Facebook), in California, has developed its own AI network, called ESMFold, and used it to predict the shapes of roughly 600 million possible proteins from bacteria, viruses and other microorganisms that have not been isolated or cultured. Scientists are using these tools to dream up proteins that could form the basis of new drugs and vaccines.Monkeypox goes global

The monkeypox virus (shown here as a coloured transmission electron micrograph) is related to the smallpox virus.Credit: CDC/Science Photo Library

The rapid global spread of monkeypox (recently renamed ‘mpox’ by the World Health Organization) this year caught many scientists off guard. Previously, the virus had mainly been confined to Central and West Africa, but from May this year, infections started appearing in Europe, the United States, Canada and many other countries, mostly in young and middle-aged men who have sex with men. The virus is related to smallpox, and the circulating strain only rarely causes severe disease or death. But its fast spread led the World Health Organization to declare the global outbreak a ‘public-health emergency of international concern’, the agency’s highest alert level, in July.As cases soared, researchers got to work trying to understand the dynamics of the disease. Studies confirmed that it is transmitted primarily through repeated skin-to-skin contact, and trials of possible treatments got under way. Existing smallpox vaccines were also used to suppress the virus in some countries. Six months after mpox infections first started increasing, vaccination efforts and behavioural changes seemed to have curbed its spread in Europe and the United States. Researchers predict a range of scenarios from here — the most hopeful being that the virus fizzles out in non-endemic countries over the next few months or years.The Moon has a revivalThe Moon has become a popular destination for space missions this year. First off the launch pad, in August, was South Korea’s Danuri probe, which is expected to arrive at its destination in January and orbit the Moon for a year. The mission is the country’s first foray beyond Earth’s orbit and is carrying a host of experiments.Last month, NASA’s hotly anticipated Artemis programme — which aims to send astronauts to the Moon in the next few years — finally kicked off with the launch of an uncrewed capsule called Orion, a joint venture with the European Space Agency. As part of a test flight to see whether the system can transport people safely to the Moon, the capsule flew out past the Moon and made its way back to Earth safely this month.A lunar spacecraft made by a Japanese company launched this month. ispace’s M1 lander is aiming to be the first of several private ventures to land on the surface of the Moon next year. The lander will carry two rovers, one for the United Arab Emirates and another for the Japan Aerospace Exploration Agency, JAXA. The rovers will be a first for both countries.Climate-change funding

People cross a flooded highway in Sindh province, Pakistan in August.Credit: Waqar Hussein/EPA-EFE/Shutterstock

There were many reasons to feel despondent about the United Nations Climate Change Conference of the Parties (COP27) in Egypt last month, but an agreement on a new ‘loss and damage’ fund was one bright spot. The fund will help low- and middle-income countries to cover the cost of climate-change impacts, such as the catastrophic floods in Pakistan this year, which caused more than US$30 billion worth of damage and economic losses.But calls at COP27 to phase out fossil fuels were blocked by oil-producing states, and many blamed the lack of progress on the energy crisis sparked by Russia’s invasion of Ukraine. High natural-gas prices have led some European nations to rely temporarily on coal. Global carbon emissions from fossil fuels are expected to hit 37.5 billion tonnes this year, a new record. The window to limit warming to 1.5–2 ºC above pre-industrial temperatures is disappearing fast — and might even have passed.Omicron’s offspring drive the pandemicOmicron and its descendants dominated all other coronavirus variants this year. The fast-spreading strain was first detected in southern Africa in November 2021, and quickly spread around the globe. From early on, it was clear that Omicron could evade immune-system defences more successfully than previous variants, which has meant that vaccines are less effective. Throughout the year, a diverse group of immune-dodging offshoots of Omicron has emerged, making it challenging for scientists to predict coming waves of infection.Vaccines based on Omicron variants have been rolled out in some countries in the hope they will offer greater protection than previous jabs, but early data suggest the extra benefit is modest. Nasal sprays against COVID-19 have also become a tool in the vaccine arsenal. The idea is that these stop the virus at the site where it first takes hold. In September, China and India approved needle-free COVID-19 vaccines that are delivered through the nose or mouth, and many similar vaccines are in various stages of development.Pig organs transplanted into people

Surgeons in Baltimore, Maryland transplanted the first pig heart into a person in January.Credit: EyePress News/Shutterstock

In January, US handyman David Bennett became the first person to receive a transplanted heart from a genetically modified pig — a crucial first step in determining whether animals could provide a source of organs for people who need them. Bennett survived for another eight weeks after the transplant, but researchers were impressed that he lived for that long, given that the human immune system attacks non-genetically modified pig organs in minutes. A few months later, two US research groups independently reported transplanting pig kidneys into three people who had been declared legally dead because they did not have brain function. The organs weren’t rejected and started producing urine. Researchers say the next step is clinical trials to test such procedures thoroughly in living people.Elections and science

Luís Inácio Lula da Silva was elected president of Brazil in October.Credit: Fabio Vieira/FotoRua/NurPhoto via Getty

National elections in Brazil, Australia and France brought relief for many researchers. After three years of science-damaging policies under right-wing president Jair Bolsonaro, Brazil narrowly elected leftist labour leader and former president Luiz Inácio Lula da Silva to lead the country in October. Scientists are hopeful that Lula’s return will result in a desperately needed boost to research funding and greater protection for the Amazon rainforest.French researchers were buoyed by President Emmanuel Macron’s victory over far-right candidate Marine Le Pen in April, and the election of Anthony Albanese as prime minister in Australia in May was seen as a good thing for science and climate-change action, too. In China, Xi Jinping cemented his legacy with an historic third term as head of the Chinese Communist Party. Xi has placed science and innovation at the heart of his country’s growth strategy.In other nations, it was unclear how research would fare under new leaders, such as Giorgia Meloni, the far-right candidate elected as Italy’s first female prime minister in October. Science was not a priority for the United Kingdom’s three prime ministers this year, although they have retained previous commitments to raise research funding. After Boris Johnson reisgned, Liz Truss was in the position for just seven weeks before she too resigned and the current Prime Minister Rishi Sunak took over.Environmental push beginsThis week, conservation and political leaders are attempting to finalize a global deal to protect the environment. The UN’s Convention on Biological Diversity Conference of the Parties (COP15) is under way in Montreal, Canada. A new biodiversity treaty, known as the post-2020 Global Diversity Framework, has been delayed by more than two years because of the COVID-19 pandemic. Progress towards an agreement has been slow, and the deal looked under threat when negotiations stalled over financing during international talks in Nairobi in June. Financial pledges from some nations to support biodiversity helped discussions to move forward, but estimates suggest that US$700 billion more is needed annually to protect the natural world. At the meeting, delegates will hopefully agree on targets to stabilize species’ declines by 2030 and reverse them by mid-century. More

Mild shading facilitates sesquiterpenoid accumulation and growth in Atractylodes lancea rhizomeTo determine a concrete shading value for the production of high-quality and high-yielding AR, we examined the major compounds, including the sesquiterpenoids hinesol (Hin), β-eudesmol (Edu), and atractylone (Atl), and the polyacetylene atractylodin (Atd), as well as the biomass of AR at different growth stages (Fig. 1A–C) under various light intensities. The sum of these four volatile oils as the total volatile oil content was subsequently analyzed. The results revealed that the accumulation of volatile oils was significantly different (p  More

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Sampling strategies and dataThe inner Oslofjorden phytoplankton dataset is a compilation of data mostly assembled from the monitoring program, financed since 1978 by a cooperation between the municipalities around the fjord, united in the counsel for technical water and sewage cooperation called “Fagrådet for Vann- og avløpsteknisk samarbeid i Indre Oslofjord”. The monitoring program started in 1973 and is ongoing. The program has sampled environmental parameters and chlorophyll since 1973, but for the first 25 years, phytoplankton data is only reported for the years 1973, 1974, 1988/9, 1990, 1994 and 1995. Since 1998, yearly sampling has been conducted, and from 2006 to 2019, the sampling frequency was approximately monthly. In addition, we have compiled research and monitoring data from researchers at the University of Oslo from 1896 and 1916, 1933–34 and 1962–1965.The records from 1896 and 1897 were collected using zoo-plankton net13. The phytoplankton collection in 1916–1917 used buckets or Nansen flasks for sampling. From 1933 to 1984, phytoplankton samples were collected using Nansen bottles and then from 1985–2020 with Niskin bottles from research vessels. The exception is the period from 2006 to 2018 when samples were also collected with FerryBox- equipped ships of opportunity14 with refrigerated autosamplers (Table 2).Since the 1990s, quantitative phytoplankton samples have mostly been preserved in Lugol’s solution, except for spring and autumn samples in the period 1990–2000 that were preserved in formalin. The records from 1896, 1897 and 1916 were preserved in ethanol, and between 1933 and 1990, samples were preserved in formalin. Sampling strategies and methods are listed in Table 2.The records from 1896 and 1897 were quantified by weight, and taxon abundance is categorised as “rare” (r), “rather common” (+), “common” (c) and “very common” (cc)13. In 1916 and 1917, Grans filtration method15 was used, and the number was given in cell counts per litre. From 1916 to 1993, the data is reported only as phytoplankton abundance (N, number of cells per litre). For most years after 1994, the dataset includes both abundance and biomass (μg C per litre), except for 2003, 2004, 2017 and 2018. Phytoplankton was identified and quantified using the sedimentation method of Utermöhl (1958)16. Biovolume for each species is calculated according to HELCOM 200617 and converted to biomass (μg C) following Menden-Deuer & Lessards (2000)18.Data inventoryThe inner Oslofjorden Phytoplankton dataset was compiled in 2020, comprising quantitative phytoplankton cell counts from inner Oslofjorden since 1896. Previously, parts of the data have been available as handwritten or printed tables in reports and published sources19,20,21 (Fig. 2). All sources are digitally available from the University of Oslo Library, the website for “Fagrådet” (http://www.indre-oslofjord.no/) or the NIVA online report database (https://www.niva.no/rapporter). Data from 1994 and onwards have been accessed digitally from the NIVA’s databases. They are also available from client reports from the monitoring project for inner Oslofjorden from the online sites listed above.The first known, published investigation of hydrography and plankton in the upper water column of the inner Oslofjorden was by Hjort & Gran (1900)13. Samples were collected during a hydrographical and biological investigation covering both the Skagerrak and Oslofjorden. There is only one sampling event from Steilene (Dk 1), but some phytoplankton data were obtained at Drøbak, just south of the shallow sill separating the inner and outer Oslofjorden, from winter 1896 to autumn 1897. Twenty years later, Gran and Gaarder (1927)22 conducted a study that included culture experiments at Drøbak field station (at the border between the inner and outer Oslofjorden) in March – April 1916 and August – September 1917. A higher frequency investigation was carried out from June 1933 to May 1934, covering 12 stations in inner and outer Oslofjorden where phytoplankton was analysed by microscopic examination23. The extensive program (the Oslofjord Project) conducted from 1962–1964 covered many parameters, and we have extracted the data for phytoplankton. From 1973 and onward, the research vessel-based monitoring program was financed by the municipalities around the fjord, and since 2006 NIVA has supplemented the monitoring program using FerryBox ships of opportunity. Samples from 4 m depth were collected using a refrigerated autosampler system (Teledyne ISCO) connected to a FerryBox system on M/S Color Festival and M/S Color Fantasy through cooperation between NIVA and Color Line A/S. Since 2018, the FerryBox has been part of the Norwegian Ships of Opportunity Program research infrastructure funded by the Research Council of Norway.The indicated depth of 3.5–4 m is an estimated average, as the actual sampling depth depends on shipload and sea conditions.Several other research projects have sampled from inner Oslofjorden between 1886 and 2000 with different aims. Data from relevant projects reporting on the whole phytoplankton community have also been included in this database.Data compilationThe data already digitalised were compiled from MS Excel files, and other data were manually entered into the standard format in MS Excel files. All collected data were then integrated into one MS Excel database, and this file was used for upload into GBIF. Data can be downloaded from GBIF in different formats and be linked together by the measurementsorfacts table.Quality control and standardisationAfter compilation, the data were checked for errors that could occur during manual digitalisation or just the compilation process. Duplicates and zero values were removed (Fig. 2). The major quantitative unit is phytoplankton abundance in cells per litre. Due to varying scopes of sampling and the development of gear and instruments, the number of species identified may vary between projects. Some of the earliest records were registered as “present”, indicating the amount in comments.Metadata, such as geographical reference, depth and methodology accessed from papers and reports, were accessible from the data source. When data was accessed from the NIVA internal databases, the metadata information was provided by the database owners/researchers.TaxonomyThe taxonomy of microalgae is in constant revision as new knowledge and techniques for identification are developing. Several historical species names recorded in this database are synonyms of accepted names in 2021. We have used the original names in our database and matched them to accepted names and Aphia ID using the taxon match tool available in the open-access reference system; World Register of Marine Species (Worms)24. The taxon match was conducted in March 2021.The nomenclature in Worms is quality assured by a wide range of taxonomic specialists. The Aphia ID is a unique and stable identifier for each available name in the database24. We also cross-checked the last updated nomenclature in Algaebase25 (March 2022) to assign species to a valid taxon name. When Algaebase and Worms were not in accordance, Algaebase taxonomy was usually chosen except in the case of Class Bacillariophyceae.Before matching the species list, the original species names were cleaned from spelling mistakes or just spelling mismatches like spaces, commas, etc. The original name is, however, left in one column in the database. For registrations where a species identification is uncertain, e.g. Alexandrium cf. tamarense, we used only Alexandrium. For registrations where the full name is uncertain, e.g. cf. Alexandrium tamarense, we used the name and Aphia ID for higher taxa, in this case, order. For others, e.g. “pennate diatoms” or “centric diatoms“, we used the name and Aphia ID for class. When names for, e.g. order and class were not recognised automatically by the matching tool in World Register of Marine Species (WoRMS), these were matched manually. Only very few records, mostly “cysts” and “unidentified monads”, could not be matched neither automatically nor manually but were assigned to general “protists” with affiliated ID. More

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