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    Fabrication of biochar derived from different types of feedstocks as an efficient adsorbent for soil heavy metal removal

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    Pathogen evasion of social immunity

    Ant hostWe used workers of the invasive Argentine ant, Linepithema humile, as host species. As typical for invasive ants, populations of this species lack territorial structuring and instead consist of interconnected nests forming a single supercolony with constant exchange of individuals between nests40. We collected L. humile queens, workers and brood in 2011, 2016 and 2022 from its main supercolony in Europe that extends more than 6,000 km along the coasts of Portugal, Spain and France40,41,42, from a field population close to Sant Feliu de Guíxols, Spain (41° 49’ N, 3° 03’ E). Field-collected ants were reared in large stock colonies in the laboratory. For the experiments, we sampled worker ants from outside the brood chambers and placed them into petri dishes with plastered ground (Alabastergips, Boesner, BAG), subjected to their respective treatments as detailed below. Experiments were carried out in a temperature- and humidity-controlled room at 23 °C, 65% relative humidity and a 12 h day/night light cycle. During experiments, ants were provided with ad libitum access to a sucrose-water solution (100 g l−1) and plaster was watered every 2–3 d to keep humidity high.Collection of this unprotected species from the field was in compliance with international regulations, such as the Convention on Biological Diversity and the Nagoya Protocol on Access and Benefit-Sharing (ABS, permit numbers ABSCH-IRCC-ES-260624-1 ESNC126 and SF0171/22). All experimental work followed European and Austrian law and institutional ethical guidelines.Fungal pathogensAs pathogen, we used the obligate-killing entomopathogenic fungus Metarhizium, whose infectious conidiospores naturally infect ants43,44,45 by penetrating their cuticles, killing them and growing out to produce highly infectious sporulating carcasses23,46. We used a total of six strains of the two species M. robertsii and M. brunneum, all isolated from the soil of the same natural population—an agricultural field at the Research Centre Årslev, Denmark27,47, which makes host co-infections with these sympatric strains in the field likely. As in ref. 24, we used three strains of M. robertsii (R1: KVL 12-36, R2: KVL 12-38, R3: KVL 12-35) and three of M. brunneum (B1: KVL 13-13, B2: KVL 12-37, B3: KVL 13-14), all obtained from the University of Copenhagen, Denmark (B. M. Steinwender, J. Eilenberg and N. V. Meyling).We started our selection experiment by exposing the ants to a mix of the six strains in equal proportions. To this end, each strain was grown separately from monospore cultivates from its long-term storage (43% glycerol (Sigma-Aldrich, G2025) in skimmed milk, −80 °C) on SDA plates (Sabouraud-4% dextrose agar, Sigma-Aldrich, 84088-500G) at 23 °C until sporulation. Conidiospores (abbreviated to ‘spores’) were collected by suspending them in sterile 0.05% Triton X-100 (Sigma-Aldrich, X-100; in milliQ water, autoclaved) and mixed in equal amounts to a total concentration of 1 × 106 spores ml−1. Before mixing, we confirmed that all strains had ≥98% germination.We exposed worker ants individually to the fungal pathogen by dipping them into the spore suspension using clean forceps (Gebrüder Martin; bioform, B32d). Afterwards, each ant was brieftly placed on filter paper (Whatman; VWR, 512-1027) to remove excess liquid before being placed into its experimental Petri dish.Serial passage experimentWe tested for the long-term effect of social immunity on pathogen selection, in which the pathogen was serially cycled through the host in the absence or presence of social immunity while the host population remained constant.Experimental design and procedureAfter exposure to the fungal spore mix, worker ants were either kept alone (individual host treatment, n = 10 replicate lines) or together with two untreated nestmates (social host treatment, n = 10 replicate lines; Fig. 1a). Individual ants could only protect themselves by individual immunity (selfgrooming behaviour and their physiological immune system), while the attended ants experienced both individual and social immunity due to the additional allogrooming by their caregiving nestmates. Thus, comparing the two host conditions revealed the effect of social immunity.As sanitary care by the nestmates reduces the pathogens’ success to kill the exposed individuals, we had to set up more experimental dishes of the social host treatment to obtain equal numbers of sporulating carcasses under both selection treatments, from which we then collected the spores for the next host infection cycle. For the individual treatment, we exposed an average of 23 workers per cycle, while an average of 40 workers per cycle were exposed in the social host treatment. The experiment was run for 10 host passages, that is, 27 weeks. In total, 6,312 workers (2,299 in the individual and 4,013 in the social host treatment) were exposed during the course of the experiment, and 8,026 nestmates were used. To obtain the spore suspensions for the next steps, we then collected and pooled the outgrowing spores of the first 8 carcasses produced per replicate line and cycle (that is, a total of n = 800 carcasses from the individual and n = 800 carcasses from the social host treatment, over the 10 host passages). Dead nestmates were not considered (see below).In detail, at each host cycle, the freshly exposed ants were placed into Petri dishes with plastered, humidified ground (Ø 3.5 cm for the individual and Ø 6 cm for the social host condition; both Bioswisstec AG, 10035 and 10060) in the absence (individual host treatment) or presence (social host treatment) of two untreated nestmates. We checked survival daily for 8 d. Ants that died within 24 h after exposure were excluded from the experiment as their mortality could not yet have resulted from infection, but rather from treatment procedures. Ants dying from days 2 to 8 were checked for internal Metarhizium infections by surface-sterilization (washing the carcass in 70% ethanol (Honeywell; Bartelt, 24194-2.5l; diluted with water) for a few seconds, rinsing it in distilled water, incubating in 3% bleach (Sigma-Aldrich, 1056142500) in sterile 0.05% Triton X-100 for 3 min and rinsing it again three times in water48), followed by incubation in a Petri dish on humidified filter paper at 23 °C until day 13, when they were checked for Metarhizium spore outgrowth. This timeline was chosen as preliminary work showed that the exposed ants die mostly on days 4 to 8 (median day 5, for both individual and social host treatments) after exposure and that sporulation required no longer than 5 d in our experimental conditions, so that a duration of 13 d per cycle also allowed for the later dying ants to complete sporulation. Preliminary work further revealed that in cases where nestmates contracted the disease, they died at a delayed timepoint and with spore outgrowth mostly around the mouthparts. These characteristics were used to distinguish between the directly exposed ants and infected nestmates in the experiment where ants were not colour-marked. The carcasses of sporulating nestmates were excluded from further procedures. An additional control experiment using 120 sham-treated ants showed no Metarhizium outgrowth, so that all Metarhizium outgrowth in our experiment could be attributed to our experimental infections. Carcasses with saprophytic outgrowth were not considered. For each host passage and each replicate line, we collected the spores of the first 8 ants dying after day 1 from their Metarhizium-sporulating carcasses at day 13 in 0.05% Triton X-100, pooled and counted them using an automated cell counter (Cellometer Auto M10, Nexcelom Bioscience). The concentration of each pool was then adjusted to 1 × 106 spores ml−1, and was used directly (that is, in the absence of any intermediate fungal growth step on agar plates) for exposing the ants in the next host infection cycle. The ants of each host passage were thus dipped in the same spore concentration. The remaining spore suspension was frozen at −80 °C in a long-term storage for further analysis.Pathogen diversity and strain compositionWe analysed which strains were present and in which proportion after 5 and 10 passages in each of the 10 individual and 10 social replicate lines. To this end, we first extracted total DNA from the respective spore pools (n = 40), which we analysed (1) quantitatively for the respective representation of M. robertsii vs M. brunneum (using species-specific real-time PCR targeting the PR1-gene sequence; detailed below) and (2) qualitatively for which of the 6 original strains were still present in the pool (using strain-specific microsatellite analysis; detailed below). We used this first estimate of remaining strain diversity and composition of each pool to determine how many spores we had to analyse separately for their strain identity after individualization by FACS sorting and growing them individually as colony forming units (c.f.u.s). This clone-level strain identification was again performed using microsatellite analysis (n = 1,347 individualized clones from the 40 spore mixes, in addition to n = 27 spores from the 6 ancestral strains; detailed below). Such clonal separation was needed since expansion of the spore mix by growth on SDA plates was not representative of the genetic composition of the strains in the pool, due to strong strain–strain growth inhibition when growing in a mix.In detail, we extracted the DNA of the 6 ancestral strains and the 40 spore mixes (10 each for individual and social lines at passages 5 and 10), as well as of 27 individualized clones of the ancestral strains and 1,374 clones from the 40 pools of passages 5 and 10, by centrifuging 100 µl of the spore suspensions in 1.5 ml tubes (Eppendorf, 0030120086) at full speed for 1 min and discarding the supernatant. Nuclease-free water (50 µl) was added and the spores were crushed in a bead mill (Qiagen TissueLyser II, 85300) at 30 Hz for 10 min using acid-washed glass beads (425–600 µm; Sigma-Aldrich, G8772). DNA was extracted using a DNeasy blood and tissue kit (Qiagen, 69506) following the manufacturer’s instructions, using a final elution volume of 50 µl buffer AE.For the quantitative species-level analysis of the pools, we performed quantitative real-time PCR (qPCR) using primers and differently labelled probes24 that we had developed on the basis of the sequence of the PR1 gene49 (forward: 5′ TCGATATTTTCGCTCCTG, reverse 5′-TTGTTAGAGCTGGTTCTGAAG, PR1 probe M. brunneum: 5′-(6-carboxyfluorescein (6FAM))TATTGTACCTACCTCGATAAGCTTAGAGAC(BHQ1), PR1 probe M. robertsii: 5′-(hexachloro-fluorescein (HEX))AGTATTGTACCTCGATAAGCTCGGAGAC(BHQ1)). Reactions were performed in 20 μl volumes using 10 μl iQ Multiplex Powermix (Bio-Rad, 1725849), with 600 nM of each primer (Sigma-Aldrich), 200 nM of each probe (Sigma-Aldrich) and 2 μl of extracted DNA. The amplification programme was initiated with a first step at 95 °C for 3 min, followed by 40 cycles of 10 s at 95 °C and 45 s at 60 °C. Primer efficiency was above 92% for both primer/probe combinations using standard curves of 10-fold dilutions of known input amounts. Data were analysed using Bio-Rad CFX Manager software.For the strain-specific analysis of both the pools and the individualized clones, we used two microsatellite loci, Ma30750 and Ma205451. Microsatellite locus Ma307 (forward: 5′-(6FAM)CATGCTCCGCCTTATTCCTC-3′, reverse: 5′-GGGTGGCGAAGAAGTAGACG-3′) allowed distinction of all strains except two of the M. brunneum strains (B1 and B3), which were distinguished by microsatellite locus Ma2054 (forward: 5′-(6FAM)GCCTGATCCAGACTCCCTCAGT-3′, reverse: 5′-GCTTTCGTACCGAGGGCG-3′). We analysed the microsatellites by E-Gel high-resolution 4% agarose gels (ILife Technologies, G501804) and fragment length analysis (done by Eurofins MWG) using Peak Scanner software 2.For clone individualization, we used flow cytometry to sort single spores out of the 40 spore pools (and the 6 ancestral strains for comparison) on 96-well plates (TPP; Biomedica, TP-92696) containing SDA (100 µl per well). The unstained spore population was detected using the FSC (forward scatter)/SSC (side scatter) in linear mode (70 μm nozzle, FACS ARIA III, BD Biosciences, as exemplified in Supplementary Fig. 1). Purity mode was set to ‘single cell’ and spore clones were obtained by sorting 1 particle event into each well. Sorting and data analysis were performed using Diva 6.2 software. The number of spores that we obtained for microsatellite analysis varied for each replicate, as it was adjusted to the remaining strain diversity estimate that we obtained from the quantitative and qualitative analysis of the pools. In total, we analysed 4–5 clones per ancestral strain (total n = 27) and a median of 5, but up to 101 different clones for the pools (total n = 1,347), as we intensified analysis for the strains that were revealed to be present at low frequency on the basis of previous analysis.Common garden experimentExperimental design and procedureWe then tested whether the successful lines at the end of the experiment (that is, after 10 host passages) differed in their virulence (induced host mortality) and investment into transmission stages (produced spore number) depending on their selection history (individual vs social), when current host social context either reflected the selection history or not. This common garden experiment thus led to 20 matched combinations of selection history and current condition (10 each of the individual lines in current individual host conditions (individual–individual) and the social lines in current social host conditions (social–social)) and 20 non-matched conditions (10 each of the individual lines in current social host conditions (individual–social) and the social lines in current individual host conditions (social–individual)).We obtained the lines for performance of the common garden experiment by the following procedure: (1) for the 16 out of the 20 replicate lines, where a single strain was the sole remaining representative at the end of the experiment (Fig. 1b), we expanded one of the c.f.u.s grown after FACS sorting (see above) by plating on SDA; (2) for the 4 remaining replicates in which two strains had remained (two individual and two social replicate lines), we expanded one c.f.u. of each of the remaining strains and mixed the spores in their representative proportion, as determined above.Virulence and transmissionFor the 10 individual and 10 social lines, we determined the induced host mortality as a measure of virulence and the outgrowing spore number as transmission stage production under their matched and non-matched current host conditions. We exposed the workers as in the selection treatment, kept them either alone or with two untreated nestmates, and monitored their mortality daily for 8 d. Again, ants dying in the first 24 h after treatment and dying nestmates were excluded from the analysis. In total, we obtained survival data of 797 ants (19–20 ants exposed for each of the 10 replicates from each of 4 combinations of selection history and current host condition). Dead ants were treated as above and their outgrowing spores collected by a needle dipped in sterile 0.05% Triton X-100 directly from the carcass, and resuspended in 100 µl of sterile 0.05% Triton X-100. The number of spores per carcass was counted individually using the automated cell counter, as described above (n = 215; median of 5 per replicate). We excluded one outlier carcass(from replicate I5) where we expected a counting error as this single carcass showed approx. 100-fold higher spore count than the other carcasses of this replicate. Exclusion of this outlier did not affect the statistical outcome. The proportion of ants dying per replicate line for each combination of selection history and current host condition and the number of spores produced by all carcasses per replicate were respectively used as measures of virulence and transmission (mean carcass spore load per replicate plotted in Fig. 2).Allogrooming elicitation by the fungal linesWe determined the allogrooming elicited by the individual and the social lines. To this end, we exposed workers as above and observed the allogrooming performed by two untreated nestmates towards the exposed ant. In detail, we performed 3 biological replicates for each of the 20 replicate lines (n = 10 individual and 10 social lines, resulting in a total of 60 videos), where the exposed ant was placed with two untreated nestmates within 10 min after exposure, and filmed with Ueye cameras for 30 min (whereby 4 cameras were used in parallel, each filming 3 replicates simultaneously, and using StreamPix 5 software (NorPix 2009-2001) for analysis). Videos were obtained in a randomized manner and labels did not contain treatment information so that the observer was blind to both the selection history and individual treatment during the behavioural annotations. For each ant, we observed both self- and allogrooming. Start and end times for each grooming event were determined, supported by use of the software BioLogic (Dimitri Missoh, 2010 (https://sourceforge.net/projects/biologic/)).As the ants in our serial passage and common garden experiments were not colour-marked, we also used unmarked ants for this behavioural experiment to keep conditions the same. This was possible as preliminary data with colour-coded nestmates (n = 18 videos) had shown that exposure alters the ant’s behaviour and that of its untreated nestmates in a predictable way that allows reliable classification of the pathogen-exposed individuals from the untreated nestmates; we used the following rules to classify an ant as the exposed individual: (1) the individual spent >5% more time (of the 30 min observation period) selfgrooming than the other individuals; (2) if the difference in selfgrooming time between the individuals was More

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    Short-term sedimentation dynamics in mesotidal marshes

    No plants were collected or harmed during this study, and all research involving plants followed relevant national, and international guidelines and legislation.Study areaThe study site encloses a wetland area bordering Ramalhete Channel, in the western part of the Ria Formosa lagoon, a mesotidal system located in southern Portugal (Fig. 1). Lunar tides are semi-diurnal, with a mean tidal range of about 2 m that can reach up to 3.5 m during spring tides. Offshore waves have no major propagation inside the lagoon33,34. Water circulation inside the lagoon is mostly driven by tides. The lagoon extends over 55 km along the coast and is connected to the ocean through six tidal inlets35. The three westmost inlets of the system (Ancão, Faro-Olhão, and Armona), which together capture ca. 90% of the total prism, are highly interconnected, with a strong residual circulation from Faro-Olhão Inlet directed towards Ancão and Armona inlets (located in Fig. 1), during both spring and neap tides36. The tidal currents in Ramalhete Channel, connecting the Faro-Olhão and Ancão Inlet, have high tidal asymmetry and shifts in tidal dominance, from flood to ebb. There are no significant fluvial inputs into the lagoon, with a yearly average terrestrial sediment influx of around 2 × 105 m3/yr37, reaching the system through small streams. The main sediment delivery to the system is through the inlets, though there are few studies assessing related fluxes. The net sediment entry through the stabilized Faro-Olhão Inlet is estimated at 1.4 × 105 m3/year38. Recent sedimentation rates in the marsh of the westmost edge of the lagoon were estimated at 1.1 ± 0.1 mm/yr39.The lagoon system is composed of large salt marsh patches, tidal flats and a complex net of natural, and partially dredged tidal channels. The tidal flats (vegetated and non-vegetated) and salt marshes represent more than 2/3 of the total lagoon area. The salt marshes comprise silt and fine sand40, while coarser (sand to shingle) shell-rich sediment, of marine provenance, is found on tidal channels and the lower domain of intertidal flats41. The dominant intertidal species are Spartina maritima and the seagrass Zostera noltei, the latter occupying an estimated area of 1304 ha, which represent 45% of the total intertidal area42.Figure 1Location of the field site in the Ria Formosa lagoon western sector over a satellite image collected in 2019 (South Portugal; upper panel); zoom to monitoring stations S1 to S4 (left lower panel); and field view of the studied site (right lower panel). Map generated with ArcGIS 10.8 (http://www.esri.com) and Adobe Illustrator 2022. Map data: Google Earth 7.3, image Landsat / Copernicus.Full size imageExperimental setup and data analysisAn experimental setup was deployed in the study area to assess dominant local topography, hydrodynamics (water levels and current velocities), Suspended Sediment Concentrations (SSCs), Deposition Rates (DRs), vegetation characteristics, and bed sediment grain size and organic matter content. Measurements were made during a full tide cycle, on a spring tide (tidal range = 3.2 m), and on a neap tide (tidal range = 1.8 m). Sampling was conducted in four wetland stations: S1 and S2 in a vegetated tidal flat comprising Zostera noltei; S3 in the low marsh comprising Spartina maritima; and S4 in the mid-upper marsh with the most abundant species of Sarcocornia perennis and Atriplex portucaloides (see S1 to S4, Fig. 1); the tidal flat is interrupted by a small oblique secondary tidal creek that flows near S2 station.Stations of sediment sampling and equipment deployment along the transect are illustrated in Fig. 2. During neap tide there was no data collection in S4, since the inundation time of the station was very short. The profile elevation was measured using Real Time Kinematic Differential Global Positioning System (RTK-DGPS, Trimble R6; vertical error in the order of few centimetres), and the slope of each habitat within a transect was calculated and expressed in percentage (%). Vegetation at each point was characterized by the canopy height, calculated as the average shoot length.Suspended Sediment Samplers (SSSs) were installed during low tide in the monitored stations using siphon samplers (Fig. 2) and recovered in the next low tide. These samplers consist of 0.5 L bottles with two holes on the cap, one for water intake and the other for air exhaust, according to the method described in13. Each intake tube is adjusted to form a siphon (i.e., inverse U), allowing to control the water level at which intake starts. Siphons were aligned at the same elevation along the transect for spring and neap tides, which means that all SSSs were collecting at the same time within the tidal cycle. During spring tide, in S1 and S2 at the tidal flat, SSSs were sampling at 0.1, 0.9, and 1.2 m from the bed, while at S3 SSSs were sampling at 0.7 and 1.0 m from the bed, and at S4 the SSS was sampling at 0.1 m from the bed (Fig. 2). During neap tide, in S1 and S2, SSSs were sampling at 0.1 and 0.9 m from the bed, while at S3 the SSS was sampling at 0.7 m from the bed.Surficial sediment samples were collected in each habitat to characterize the sediment grain size (d50) and content of organic matter (% OM). Sediment traps were installed in 3 replicates, during low tide, at each sampling point to measure the short-term sediment deposition rate (i.e., deposition over a tidal cycle, following procedures of43). Traps consisted of 3 cm diameter pre-labeled cylindrical tubes (Falcon® tubes, 50 ml). Traps and sediment samples were transported to the laboratory and maintained in a fridge. The sediment content was washed, and both the inorganic and organic weights were determined.The measured inorganic DR (g/m2/hr) was calculated as:$${text{DR}} = {raise0.7exhbox{${{text{W}}_{{{text{DS}}}} }$} !mathord{left/ {vphantom {{{text{W}}_{{{text{DS}}}} } {{text{A}} cdot {text{T}}}}}right.kern-0pt} !lower0.7exhbox{${{text{A}} cdot {text{T}}}$}}$$
    (1)
    where WDS is the weight of deposited sediment (in grams), A is the area of the sediment trap opening (m2), and T is in hours. Two different tide durations were considered to compute DRs, one assuming T equal to the hydroperiod in each station, and one assuming T equal to the entire tide duration (~ 12.4 h). These measured DRs are hereon mentioned as flood and tide DRs (DRflood and DRtide, respectively). The former is an expression of the actual deposition rate within the flood phase, during the period in which each station is inundated (and therefore active deposition can take place). The latter is the value used to compare with DRs in literature, which typically corresponds to values averaged over multiple tidal cycles (thus accounting for the entire tide duration).Tide levels were measured in the field using pressure sensors (PT, InSitu Inc. Level TROLL; ~ 2 cm from the bed), deployed from S2 towards S4 (Fig. 2). Velocity currents were measured at 20 cm from the bed, using an electromagnetic current meter (EMCM; Infinity Series JFE Advantech Co., Ltd; in S2 to S4; Fig. 2), and raw data (recording interval: 30 s) were filtered using a 10 min moving average for cross-shore and longshore components. To identify tidal asymmetry and assess the related phase dominance, tidal current skewness was calculated through the formula described in44 by which:
    $$Sk_{U} = frac{{frac{1}{N – 1}mathop sum nolimits_{t = 1}^{N} left( {U_{t} – overline{U}} right)^{3} }}{{left( {frac{1}{N – 1}mathop sum nolimits_{t = 1}^{N} left( {U_{t} – overline{U}} right)^{2} } right)^{{{raise0.7exhbox{$3$} !mathord{left/ {vphantom {3 2}}right.kern-0pt} !lower0.7exhbox{$2$}}}} }}$$
    (2)
    where N is the number of recordings, Ut is the input velocity signal and (overline{U}) is the mean velocity. Positive/negative skewness indicates flood/ebb dominance (assuming that flood currents are positive).Figure 2Deployment of the sediment traps, SSSs and devices (electromagnetic current meter EMCM; pressure transducer PT) in the stations (S1 to S4) during spring tide (sketch is exaggerated in the vertical).Full size imageComplementary to the measured DRs, theoretical DRs were also determined from the data, allowing us to link the sediment and flow data collected, and validate the deposition patterns from the traps. The theoretical deposition rate was determined based on45 formula:$${text{DR}} = left{ {begin{array}{*{20}c} {{text{C}}_{{text{b}}} cdot {text{w}}_{{text{s}}} cdot left( {1 – frac{{{uptau }_{{text{b}}} }}{{{uptau }_{{{text{cd}}}} }}} right)} & {{uptau }_{{text{b}}} < {uptau }_{{{text{cd}}}} } \ 0 & {{uptau }_{{text{b}}} ge {uptau }_{{{text{cd}}}} } \ end{array} } right.$$ (3) where Cb is the SSC at the bed, ws is the flock settling velocity, τb is the bed shear stress and τcd is the corresponding critical value for deposition.To determine the settling rate of the flocculates, the modified Stokes’ velocity for cohesive sediment was used, taking shape factors α and β (α = β = 1 for perfectly spherical particles):$${text{w}}_{{text{s}}} = frac{{upalpha }}{{upbeta }} cdot frac{{left( {{uprho }_{{text{s}}} - {uprho }_{{text{w}}} } right) cdot {text{g}} cdot {text{D}}_{50}^{2} }}{{{uprho }_{{text{w}}} cdot 18 cdot {upnu }}}$$ (4) where ρw and ρs are the densities of the water and sediment, respectively and ν is the kinematic viscosity of water (~ 106 m2/s).The bed shear stress τb was calculated from the measured current magnitude, |U| using the law of the wall:$$begin{array}{*{20}c} \ {{uptau }_{{text{b}}} = {uprho }_{{text{w}}} cdot {text{u}}_{*}^{2} , {text{u}}_{*} = frac{left| U right| cdot kappa }{{ln left( {{raise0.7exhbox{$z$} !mathord{left/ {vphantom {z {z_{0} }}}right.kern-0pt} !lower0.7exhbox{${z_{0} }$}}} right)}} } \ end{array} { }$$ (5) where κ is the von Kármán constant (~ 0.4) and z0 is the roughness length. For Zostera noltei, the roughness length was estimated at 5 mm46, value that was also used in the other stations, in lack of related estimate for marsh plants.The critical shear for deposition, τcd, was calculated using the formula47:$$sqrt {frac{{{uptau }_{{{text{cd}}}} }}{{{uprho }_{{text{w}}} }}} = left{ {begin{array}{*{20}c} {0.008} & {{text{w}}_{{text{s}}} le 5 cdot 10^{ - 5} {text{m}}/{text{s}}} \ {0.094 + 0.02 cdot {text{log}}_{10} left( {{text{w}}_{{text{s}}} } right)} & {3 cdot 10^{ - 4} le {text{w}}_{{text{s}}} le 5 cdot 10^{ - 5} {text{m}}/{text{s}}} \ {0.023} & {{text{w}}_{{text{s}}} ge 3 cdot 10^{ - 4} {text{m}}/{text{s}}} \ end{array} } right.$$ (6) Theoretical values of minimum SSCs needed for these DRs were also calculated, assuming that there is constant deposition (i.e., setting τb = 0), and compared with the field results. More

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    Analysis of Himalayan marmot distribution and plague risk in Qinghai province of China using the “3S” technology

    Himalayan marmot habitat analysisThe environmental factors including temperature, vegetation and elevation are the key drivers for the wildlife in alpine ecosystems32. Specific landform attributes such as slope and elevation and vegetation cover affect the population and burrowing of rodents33. For example, rodent burrows in the Western Usambara Mountains in Tanzania were only found at an elevation of above 1600 m33. However, the Himalayan marmot seems to prefer to inhabit areas with low elevation and high land surface temperature34. In this study, the data showed that 76.25% of the Himalayan marmots were found in areas with elevation values of 3400–4600 m. The majority of marmots were found in areas with slopes of 5–20° and vegetation cover higher than 60%. Most marmots were found in alpine meadows, a few were found in temperate grasslands and alpine grasslands, and none were found in other grassland types.Preliminary statistical analysis of vegetation cover, grass type, vegetation type, and Himalayan marmot distribution sample sites obtained using spatial geographic information technology revealed that the meadow grassland areas with lush grass growth, more dominant plants, and abundant food had more marmots. When the vegetation cover reached 0.60–1.00, the number of marmot distribution sample sites was the highest. Dense grass is an ideal habitat and provides concealment for Himalayan marmots, and the abundant plant types provide sufficient food for marmots. In contrast, no marmots were distributed in the alpine scrub, coniferous forest, and alpine snow/ice covered areas where vegetation growth was poor, vegetation cover was low, and food was relatively scarce. Moreover, 70.24% of Himalayan marmots were found in alpine meadows with a wide variety of plant species, including Poaceae, Cyperaceae, and grasses. This finding indicated that alpine meadows are more suitable for Himalayan marmots and have more advantageous habitat conditions compared with other grassland types. The elevation of alpine meadows is 3236–5126 m, and the vegetation is mainly meadows with simple vegetation structure, substantial vegetation cover and dense vegetation growth, and a wide variety of plants, rich food, soft grass, and good palatability. Therefore, alpine meadows provide good natural habitats and foraging sites for marmots.Habitat selection of large rodents is influenced by a combination of vegetation cover availability, food availability, and population density35. Vegetation cover is an important parameter that describes vegetation communities and ecosystems and is closely related to vegetation quantity and productivity. The quality of habitat vegetation is an important factor that affects the spatial distribution of plateau rodents. Both feeding and concealment depend on vegetation, and the height and cover of edible plants and vegetation suitable for concealment determine the choice of vegetation type by marmots. Thus, vegetation cover becomes an important factor for habitat selection by marmots. Different grassland types determine different plant conditions, and selection of different vegetation conditions can increase the chances of survival and improve the reproductive success of marmots; therefore, grassland type is an important ecological factor in habitat selection by marmots. A study showed that the ecological factors affecting habitat selection of Himalayan marmots are mainly topography, anthropogenic disturbance, and vegetation8. Another study concluded that habitat selection by Himalayan marmots is closely related to elements such as topography, landform, temperature, precipitation, and vegetation24.The functions of burrows’ physical parameters is to protect the Himalayan marmots from natural enemies and bad weather36. There is clearly influence of slope on habitat selection by marmots. When the slope is large, wind is strong, and burrows are not well hidden; this makes them difficult to defend against enemies, unsafe for survival, and not conducive to hibernation during winter. In addition, Himalayan marmots prefer to burrow on sunny aspect, because the temperature is suitable and the vegetation is lush, which is suitable for marmots to breed. Therefore, the number of marmot burrows gradually decreases with increasing slope and ubac. Although flat and low-lying areas with small slopes are good for marmots to create dens, rainwater will easily flow into the dens during summer rainfall, which will kill marmots. Therefore, a suitable slope and sunny aspect are also very important for habitat selection by marmots.Application of the predictive spatial distribution map of Himalayan marmots in Qinghai provincePlague surveillance is the main measure used for plague prevention and control in China. Although we have made many improvements in plague surveillance, the traditional method of dragnet surveillance still consumes a lot of human and material resources, is inefficient. The pasture area of Qinghai province is approximately 380,000 km2, and the identified natural plague focus is approximately 180,000 km2; therefore, there is still 200,000 km2 of pasture where the distribution of Himalayan marmots and plague have not been identified. Currently, RS technology is widely used in the fields of mapping and ecological surveillance18,19,21,22,37.Applications of RS technology in areas such as malaria, dengue, schistosomiasis and plague have been previously reported27,37. Using GIS combined with remotely sensed data, Proches Hieronimo et al. found that the presence of small mammals was positively influenced by elevation, whereas the presence of fleas was clearly influenced by land management features, and thus these observations have positive implications for plague surveillance27. In this study, RS technology combined with field validations were used to determine the distribution and areas of different types of grasslands in Qinghai province, and the average density of Himalayan marmot distribution in different types of grasslands. The high-, low-, and very low-density areas of Himalayan marmot distribution were identified. The soil map, vegetation map, administrative map, and marmot density statistics were merged to form the spatial data and attribute data basis for the information system to map the distribution of Himalayan marmot and determine the area of Himalayan marmot distribution. Generally speaking, the occurrence of human plague epidemic is closely related to the local animal plague epidemic2. However, a large part of the high-density distribution of Himalayan marmots is located in uninhabited areas and the areas are generally sparsely populated, which also indicates that we should reasonably allocate plague prevention and control resources to areas where human plague is most likely to occur to prevent the occurrence of human plague epidemics.Field validation for verificationThrough field validation and information from local farmers and herdsmen, we confirmed that Himalayan marmots inhabited 68 sample sites in Tongde, Zeku, Guinan, Xunhua, Haiyan, Ulan, Qilian, Hualong, and Huzhu counties. Among them, Tongde, Zeku, Guinan, Xunhua, Haiyan, Ulan, and Qilian counties have all historically experienced marmot plague outbreaks and can be considered as reliable natural plague foci38. The data from this field validation are consistent with the previous survey data and the epidemic history of the counties in Qinghai province39.MAE can better reflect the actual number of errors in prediction values; the smaller the MAE value, the higher the prediction accuracy. The MAE derived from the field validation data was 0.1331 and the prediction accuracy was 0.8669. The accuracy of the predicted Himalayan marmot spatial distribution reached 87%, which indicated that the predicted probability map of the Himalayan marmot spatial distribution can better predict the potential marmot distribution.The predicted spatial distribution map of Himalayan marmot in Qinghai province was then compared with environmental information such as elevation, vegetation, grass type, slope, and aspect of 352 field survey sites. The obtained RS data showed that the prediction results were excellent, and the predicted spatial distribution map of Himalayan marmot in Qinghai province was drawn with high accuracy. The prediction map visually reflects the different density distribution of Himalayan marmots; this allows us to optimize the settings and reasonable spatial layout of animal plague surveillance sites and improve surveillance efficiency.Application of marmot information collection system V3.0Marmot information collection system V3.0 was developed based on the “3S” technology standardizing the collection of surveillance data, and makes the management and analysis of information more convenient and faster. This study revolutionized the traditional method of considering plague-stricken counties as the plague foci, and effectively reduces the work intensity of operators and improves the data collection efficiency. In 2016 and 2017, we applied this system to the animal plague surveillance tasks in the plague-stricken counties of Haidong, Hainan, and Haibei in Qinghai province, and standardized the collection of provincial geographic location data of animal plague surveillance (data not shown). In 2018, we also applied this system in Wulan County, which frequently experiences plague, and achieved a good application effect (data not shown).In the next step, we will expand the pilot areas (mainly national and provincial plague surveillance sites), collect surveillance data from each surveillance site, continuously optimize and update the system, improve the efficiency of data analysis and utilization, detect the plague epidemic in marmot in a timely and accurate manner, correctly determine the epidemic trend of plague in marmots, and attempt to strictly prevent the plague from spreading to humans. We plan to use a new model of drone surveillance to create a multidimensional, three-dimensional, real-time big data plague surveillance information reporting system to enhance early plague warnings and prediction in Qinghai province and even in the country, which will be of positive practical significance to serve and guarantee the Belt and Road Initiative. These approaches are expected to provide new technical means for plague investigation and research, and to provide references for setting up plague surveillance programs and prediction for the natural Himalayan marmot plague focus in Qinghai province and the QTP. More

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    The temperature dependence of microbial community respiration is amplified by changes in species interactions

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