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    Arbuscular mycorrhizal fungi in oat-pea intercropping

    Biomass yield and WUEBiomass increased with precipitation, as expected and reported in Lee3. There was less water uptake in intercropping compared to sole oat and pea. In general, intercropping represented the median of the two sole cropping treatments, where oat had the highest biomass and WUE while pea had the lowest, and where pea had the highest mineral content and oat had the lowest3. Intercropping resulted in advantages in forage yield stability and was not associated with changes to the AMF community.Alpha diversityWe found differences in AMF species richness estimates in the roots across treatment combinations (i.e., intercropping systems × N fertilizer rate) in 2019 (Chao1, p  More

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    Molecular analyses of pseudoscorpions in a subterranean biodiversity hotspot reveal cryptic diversity and microendemism

    Zupan Hajna, N. Dinaric karst: Geography and geology in Encyclopedia of Caves (eds. White, W. B. & Culver, D. C.) 195–203 (Academic Press, 2012).Jug-Dujaković, M., Ninčević, T., Liber, Z., Grdiša, M. & Šatović, Z. Salvia officinalis survived in situ Pleistocene glaciation in ‘refugia within refugia’ as inferred from AFLP markers. Plant Syst. Evol. 306, 1–12 (2020).Article 

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    Geomagnetic disturbance associated with increased vagrancy in migratory landbirds

    To investigate whether vagrancy is associated with geomagnetic disturbance and solar activity, we developed a method for quantifying the relative vagrancy of spatiotemporal records for 152 North American landbird species (nfall = 150, nspring = 124). While vagrancy is often treated as a binary classification (i.e., an individual is either a vagrant or not) and then used as a discrete variable (i.e., a count of total vagrants in an area)16,18, here we calculated it as a continuous variable by combining two large-scale ornithological datasets—captures and encounters of individually marked birds from the USGS Bird Banding Lab (BBL)49 and weekly, species-specific abundance maps for the continental United States from the eBird Status and Trends (hereafter, eBird S&T; via the R package ‘ebirdst’, version 2.1.0)69. Banding records have the advantage over other potential databases of vagrancy records (such as eBird or rare bird lists) in that efforts are long-term, continent-wide, have limited false positives, and have only one record per individual. Additionally, eBird S&T has the advantage over static range maps in that they provide weekly predictions and incorporate relative abundance. With these two data sources, we constructed a species-specific vagrancy value (Fig. S1), that measures the spatiotemporal rarity for every banding record. Inclusion of all banding records rather than just rare records allowed for the analysis of the dispersion of whole species populations, mitigating the potential bias of effort in banding operations (i.e., more vagrant records with greater effort). We then used hierarchical Bayesian random-effects models to estimate the strength of the association between geomagnetic disturbance, solar activity, and avian vagrancy.Species data and inclusionWe considered all full—or partial-migrant landbird species with a breeding, non-breeding, or migratory range in the United States or Canada. To do this, we used species distribution maps accessed through Birds of the World70. Landbird species likely to be caught through banding efforts (excluding species like raptors, nightjars, and swifts) that regularly occur in  > 3 but  10 km. Each banding record included the date, latitude and longitude (and precision), species, and age (if known;71). Banding records were filtered to those captures that occurred during the species-specific migration period as defined by eBird S&T69. eBird S&T approximates stationary and migratory periods by determining when the distribution of whole species population is moving69. Our use of banding records within species-specific eBird S&T migratory periods was designed to maximize the proportion of migrant birds in the analysis, but likely excludes some early and late records of migrating individuals.Banding records of species that underwent taxonomic divisions or aggregations during the study period were eliminated if the date occurred during a period in which the species identity according to modern taxonomy is indeterminate (see Supplement 2). Taxonomic reclassifications were not considered when species divisions/aggregations would only affect records from outside North America, such as the split of a Southern American taxon, Chestnut-collared Swallow (Petrochelidon rufocollaris) from its North American counterpart, Cave Swallow (Petrochelidon fulva). In these cases, we assumed all banding records during the study period were of the North American species. For a full list of periods where species records were excluded, see Supplement 2. Species with  More

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    Tracking the British agricultural revolution through the isotopic analysis of dated parchment

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    Exposure of aquatic organisms to natural radionuclides in irrigation drains, Qena, Egypt

    Samples collection and preparationFreshwater and sediment samples were collected from 5 irrigation drains (EL-Shikah, EL- Tramsa, EL-Mahrosa, EL-Aslia, and EL-Rawy) located in the geographical area of Qena city, the capital of Qena Governorate, 600 km south of Cairo, (Figs. 1 and 2). 3 sites inside each drain were randomly selected as sampling site; one of these sites represents the outlet of the drain into the Nile River. In addition, one site facing each drain in the main stream of the Nile River was selected to collect freshwater only, thus the total number of samples are 20 freshwater and 15 sediment samples.Figure 1Location map of the area under study (ArcGIS software 10.8.1; ArcGIS Online).Full size imageFigure 2Irrigation drain under study.Full size imagePolyethylene Marinelli beakers with a capacity of 1.4 L are used as collection and measuring containers. The beakers were washed with dilute hydrochloric acid and distilled water before use, filled to brim, and then pressed the tight lid to eliminate the internal air. Drops of HNO3 were added to the samples to prevent the adhesive of radionuclides with bottle walls8.Sediment samples were collected by Ekman grab sediment sampler. The collected samples were dried using electrical oven at a temperature of 105℃ for 24 h, then sieved through 200 mesh size. The dried samples were filled in hermetical sealed 500 ml polyethylene beakers. The prepared water and sediment samples were stored for 4 weeks to reach a secular equilibrium of radium and thorium with their progenies9.Measuring systemsGamma-ray spectrometer consisting of ″3 × 3″ NaI (Tl) detector enclosed in 5 cm thick cylindrical lead shield to reduce the background radiation and connected with 1024 multichannel analyzer was used. The spectrometer was calibrated for energy using 60Co and 137Cs standard point sources, and calibrated for efficiency using a multi-nuclides standard solution which covers a wide range of energy10. The spectrum was accumulated from each sample over 24 h and analyzed by Maestro software. The background was measured under the same condition of sample measurement.226Ra was determined using 214Bi and 214Pb gamma-lines at 609 keV and 352 keV, respectively, while 232Th from gamma-lines of 228Ac (911 keV) and 212Pb (238 keV). 40K was determined from its single gamma-line at 1460 keV. The activity concentration was calculated using the following formula (Eq. 1)11.$$A = frac{{C_{n} }}{{T times varepsilon { } times {text{P}} times {text{V }}left( {{text{or}}} right){text{M}}}}$$
    (1)

    where A is the activity concentration (Bq kg−1) or (Bq l−1), Cn is the net counts under a given peak area, T the sample counting time, (varepsilon) is the detection efficiency at measured energy, P is the emission probability and V is the sample volume in liter, M is the sample mass in kilogram. Minimum detectable activity (MDA) was estimated according to Currie definition using Eq. 212 and the MDA values were 0.031, 0.035 and 1.94 Bq L−1 for 226Ra, 232Th, and 40K, respectively.$${text{MDA}} = frac{2.71 + 465sqrt B }{{T times varepsilon times P times V}}$$
    (2)

    where B is the background counts under a given peak area,T,ɛ, P, and V are defined above.Doses for aquatic organismsThe external and internal absorbed dose rate for aquatic organisms (Phytoplankton, Mollusca, and Crustacean) in the studied irrigation drains was calculated based on the measured activity concentrations of 226Ra, 232Th, and 40K in environmental media (water and sediment) and using dose conversion coefficients of a given radionuclide for the reference organisms according to the method outlined by Brown et al. described below13,14.$$begin{aligned}& left( {Sediment,, conc. ,,wet} right)_{radionuclide} = (Sediment ,,conc. ,,dry)_{radionuclide} times left( {solids ,,fraction} right) \& qquad qquad + (water ,,conc.)_{radionuclide} times (1 – left( {solids ,,fraction} right). \ end{aligned}$$
    (3)
    $$begin{aligned}& left( {user2{External ,,dose ,,rate}} right)_{radionuclide,, organism} = DPUC_{radionuclide, ,organism}^{external} times left[ {Sediment ,conc. ,wet_{radionuclide} times left( {fsed_{organism} + fsedsur_{organism} /2} right)} right. \& quad quad left. { + left( {fwater_{organism} + fsedsur_{organism} /2} right) times water ,conc._{radionuclide } /1000} right] \ end{aligned}$$
    (4)
    $$left( {user2{Internal,dose,rate}} right)_{{radionuclide,,organism}} = ~left( {water,conc.} right)_{{radionuclide}} times CF_{{radionuclide}}^{{organism}} times DPUC_{{radionuclide,,organism}}^{{internal}}$$
    (5)

    where sediment conc. is the sediment activity concentration of a given radionuclide in Bq kg−1,water conc. is the water activity concentration of a given radionuclide in Bq m−3, CF is distribution coefficient factors for given radionuclide in freshwater sediment in m3 kg−1, DPUC is the dose rate per unit concentration coefficients (fresh weight) in μGy h−1 per Bq kg−1 weighted for radiation type (alpha = 10, low energy beta = 3, and high energy beta and gamma = 1), solids fraction of wet sediment (0.4), fsed organism is the time fraction spends by organism in sediment, fsedsur organism is the time fraction spends by organism at the sediment/water interface, fwater organism is the time fraction spends by organism in the water column. All parameters used in calculation are taken from Pröhl (2003)15 and Vives i Battle et al. (2004)16. The total dose is then calculated by summating the external and internal doses. More

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    Functional vegetation change over millennia

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