Holland, R. A. et al. Global impacts of energy demand on the freshwater resources of nations. Proc. Natl Acad. Sci. U.S.A. 112, E6707–E6716 (2015).CAS 
 Article 
 Google Scholar 
 Gleick, P. H. & Palaniappan, M. Peak water limits to freshwater withdrawal and use. Proc. Natl Acad. Sci. U.S.A. 107, 11155–11162 (2010).CAS 
 Article 
 Google Scholar 
 Oki, T. & Kanae, S. Global hydrological cycles and world water resources. Science. 313, 1068–1072 (2006).CAS 
 Article 
 Google Scholar 
 Postel, S. L., Daily, G. C. & Ehrlich, P. R. Human Appropriation of Renewable Fresh Water. Science. 271, 785–788 (1996).CAS 
 Article 
 Google Scholar 
 United Nations Children’s Fund (UNICEF) & World Health Organization (WHO). Progress on household drinking water, sanitation and hygiene 2000-2017. Special focus on inequalities. https://www.unicef.org/media/55276/file/Progress on drinking water, sanitation and hygiene 2019.pdf (2019).Prüss-Ustün, A. et al. Burden of disease from inadequate water, sanitation and hygiene for selected adverse health outcomes: An updated analysis with a focus on low- and middle-income countries. Int. J. Hyg. Environ. Health 222, 765 (2019).Caprioli, A., Morabito, S., Bruégre, H. & Oswald, E. Enterohaemorrhagic Escherichia coli: emerging issues on virulence and modes of transmission. Vet. Res. 36, 289–311 (2005).CAS 
 Article 
 Google Scholar 
 Vital, M., Fuchslin, H. P., Hammes, F. & Egli, T. Growth of Vibrio cholerae O1 Ogawa Eltor in freshwater. Microbiology 153, 1993–2001 (2007).CAS 
 Article 
 Google Scholar 
 Agudelo Higuita, N. I. & Huycke, M. M. Enterococcal Disease, Epidemiology, and Implications for Treatment. in Enterococci: From Commensals to Leading Causes of Drug Resistant Infection 47–72 (Massachusetts Eye and Ear Infirmary, 2014).Paton, J. C. & Paton, A. W. Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clin. Microbiol. Rev. 11, 450–479 (1998).CAS 
 Article 
 Google Scholar 
 Bellamy, W. D., Silverman, G. P., Hendricks, D. W. & Logsdon, G. S. Removing Giardia cysts with slow sand filtration. J. Am. Water Works Assoc. 77, 52–60 (1985).Fogel, D., Isaac-Renton, J., Guasparini, R., Moorehead, W. & Removing, O. J. giardia and cryptosporidium by slow sand filtration. JAWWA, Res. Technol. 3, 77–84 (1993).Article 
 Google Scholar 
 Hijnen, W. A. M., Schijven, J. F., Bonné, P., Visser, A. & Medema, G. J. Elimination of viruses, bacteria and protozoan oocysts by slow sand filtration. Water Sci. Technol. 50, 147–154 (2004).CAS 
 Article 
 Google Scholar 
 Campos, L. C., Su, M. F. J., Graham, N. J. D. & Smith, S. R. Biomass development in slow sand filters. Water Res. 36, 4543–4551 (2002).CAS 
 Article 
 Google Scholar 
 Basu, O. D., Dhawan, S. & Black, K. Applications of biofiltration in drinking water treatment – a review. J. Chem. Technol. Biotechnol. 91, 585–595 (2016).CAS 
 Article 
 Google Scholar 
 Terry, L. G. & Summers, R. S. Biodegradable organic matter and rapid-rate biofilter performance: A review. Water Res. 128, 234–245 (2018).CAS 
 Article 
 Google Scholar 
 Loh, Z. Z. et al. Shifting from conventional to organic filter media in wastewater biofiltration treatment: a review. Appl. Sci. 2021, Vol. 11, Page 8650 11, 8650 (2021).CAS 
 Google Scholar 
 Bennett, A. Drinking water: Pathogen removal from water – technologies and techniques. Filtr. Sep. 45, 14–16 (2008).CAS 
 Article 
 Google Scholar 
 Di Cristo, C., Esposito, G. & Leopardi, A. Modelling trihalomethanes formation in water supply systems. Environ. Technol. 34, 61–70 (2013).Article 
 CAS 
 Google Scholar 
 Pooi, C. K. & Ng, H. Y. Review of low-cost point-of-use water treatment systems for developing communities. npj Clean Water 2018 11 1, 11 (2018).Article 
 Google Scholar 
 Flemming, H.-C. et al. Biofilms: an emergent form of bacterial life. Nat. Rev. Microbiol. 14, 563–575 (2016).CAS 
 Article 
 Google Scholar 
 Fu, J. et al. Pilot investigation of two-stage biofiltration for removal of natural organic matter in drinking water treatment. Chemosphere 166, 311–322 (2017).CAS 
 Article 
 Google Scholar 
 Chen, F. et al. Kinetics of natural organic matter (NOM) removal during drinking water biofiltration using different NOM characterization approaches. Water Res. 104, 361–370 (2016).CAS 
 Article 
 Google Scholar 
 McKie, M. J., Ziv-El, M. C., Taylor-Edmonds, L., Andrews, R. C. & Kirisits, M. J. Biofilter scaling procedures for organics removal: A potential alternative to piloting. Water Res. 151, 87–97 (2019).CAS 
 Article 
 Google Scholar 
 de Vries, J. Soil filtration of wastewater effluent and the mechanism of pore clogging. J. Water Pollut. Control Fed. 44, 565–573 (1972).
 Google Scholar 
 Métivier, R., Bourven, I., Labanowski, J. & Guibaud, G. Interaction of erythromycin ethylsuccinate and acetaminophen with protein fraction of extracellular polymeric substances (EPS) from various bacterial aggregates. Environ. Sci. Pollut. Res. 20, 7275–7285 (2013).Article 
 CAS 
 Google Scholar 
 Writer, J. H., Barber, L. B., Ryan, J. N. & Bradley, P. M. Biodegradation and attenuation of steroidal hormones and alkylphenols by stream biofilms and sediments. Environ. Sci. Technol. 45, 4370–4376 (2011).CAS 
 Article 
 Google Scholar 
 Flemming, H.-C. Biofilms. in Encyclopedia of Life Sciences (John Wiley & Sons, Ltd, 2008). https://doi.org/10.1002/9780470015902.a0000342.pub2.Kragh, K. N. et al. Role of multicellular aggregates in biofilm formation. MBio 7, e00237 (2016).CAS 
 Article 
 Google Scholar 
 Grumbein, S., Opitz, M. & Lieleg, O. Selected metal ions protect Bacillus subtilis biofilms from erosion †. Metallomics 6, 1441 (2014).CAS 
 Article 
 Google Scholar 
 Fu, J. et al. Removal of pharmaceuticals and personal care products by two-stage biofiltration for drinking water treatment. Sci. Total Environ. 664, 240–248 (2019).CAS 
 Article 
 Google Scholar 
 Nemani, V. A., McKie, M. J., Taylor-Edmonds, L. & Andrews, R. C. Impact of biofilter operation on microbial community structure and performance. J. Water Process Eng. 24, 35–41 (2018).Article 
 Google Scholar 
 Beutel, M. W. & Larson, L. Pathogen removal from urban pond outflow using rock biofilters. Ecol. Eng. 78, 72–78 (2014).Article 
 Google Scholar 
 Wendt, C. et al. Microbial removals by a novel biofilter water treatment system. Am. J. Trop. Med. Hyg. 92, 765–772 (2015).Article 
 Google Scholar 
 Granger, H. C., Stoddart, A. K. & Gagnon, G. A. Direct biofiltration for manganese removal from surface water. J. Environ. Eng. 140, 04014006 (2014).Article 
 CAS 
 Google Scholar 
 Srivastava, N. K. & Majumder, C. B. Novel biofiltration methods for the treatment of heavy metals from industrial wastewater. J. Hazard. Mater. 151, 1–8 (2008).CAS 
 Article 
 Google Scholar 
 Fu, J. et al. Removal of disinfection byproduct (DBP) precursors in water by two-stage biofiltration treatment. Water Res. 123, 224–235 (2017).CAS 
 Article 
 Google Scholar 
 McKie, M. J., Andrews, S. A. & Andrews, R. C. Conventional drinking water treatment and direct biofiltration for the removal of pharmaceuticals and artificial sweeteners: A pilot-scale approach. Sci. Total Environ. 544, 10–17 (2016).CAS 
 Article 
 Google Scholar 
 Crognale, S. et al. Biological As(III) oxidation in biofilters by using native groundwater microorganisms. Sci. Total Environ. 651, 93–102 (2019).CAS 
 Article 
 Google Scholar 
 Klayman, B. J., Volden, P. A., Stewart, P. S. & Camper, A. K. Escherichia coli O157:H7 requires colonizing partner to adhere and persist in a capillary flow cell. Environ. Sci. Technol. 43, 2105–2111 (2009).CAS 
 Article 
 Google Scholar 
 Bauman, W. J., Nocker, A., Jones, W. L. & Camper, A. K. Retention of a model pathogen in a porous media biofilm. Biofouling 25, 229–240 (2009).CAS 
 Article 
 Google Scholar 
 Nocker, A., Burr, M. & Camper, A. Pathogens in water and biofilms. In Microbiology of waterborne diseases: microbiological aspects and risks: Second Edition 3–32 (Academic Press, 2013). https://doi.org/10.1016/B978-0-12-415846-7.00001-9.Li, J., McLellan, S. & Ogawa, S. Accumulation and fate of green fluorescent labeled Escherichia coli in laboratory-scale drinking water biofilters. Water Res. 40, 3023–3028 (2006).CAS 
 Article 
 Google Scholar 
 Rendueles, O. & Ghigo, J.-M. Mechanisms of competition in biofilm communities. Microbiol. Spectr. 3, 1–14 (2015).Hibbing, M. E., Fuqua, C., Parsek, M. R. & Peterson, S. B. Bacterial competition: Surviving and thriving in the microbial jungle. Nat. Rev. Microbiol. 8, 15–25 (2010).CAS 
 Article 
 Google Scholar 
 Aoki, S. K. et al. A widespread family of polymorphic contact-dependent toxin delivery systems in bacteria. Nature 468, 439–442 (2010).CAS 
 Article 
 Google Scholar 
 MacIntyre, D. L., Miyata, S. T., Kitaoka, M. & Pukatzki, S. The Vibrio cholerae type VI secretion system displays antimicrobial properties. Proc. Natl Acad. Sci. U.S.A. 107, 19520–19524 (2010).CAS 
 Article 
 Google Scholar 
 Ławniczak, Ł., Marecik, R. & Chrzanowski, Ł. Contributions of biosurfactants to natural or induced bioremediation. Appl. Microbiol. Biotechnol. 97, 2327 (2013).Article 
 CAS 
 Google Scholar 
 Cornforth, D. M. & Foster, K. R. Competition sensing: the social side of bacterial stress responses. Nat. Rev. Microbiol. 2013 114 11, 285–293 (2013).CAS 
 Google Scholar 
 Legnani, P., Leoni, E., Rapuano, S., Turin, D. & Valenti, C. Survival and growth of Pseudomonas aeruginosa in natural mineral water: a 5-year study. Int. J. Food Microbiol. 53, 153–158 (1999).CAS 
 Article 
 Google Scholar 
 Moll, D. M., Summers, R. S., Fonseca, A. C. & Matheis, W. Impact of temperature on drinking water biofilter performance and microbial community structure. Environ. Sci. Technol. 33, 2377–2382 (1999).CAS 
 Article 
 Google Scholar 
 Hozalski, R. M., Bouwer, E. J. & Goel, S. Removal of natural organic matter (NOM) from drinking water supplies by ozone-biofiltration. Water Sci. Technol. 40, 157–163 (1999).CAS 
 Article 
 Google Scholar 
 Schmidt, K. D., Tümmler, B. & Römling, U. Comparative genome mapping of Pseudomonas aeruginosa PAO with P. aeruginosa C, which belongs to a major clone in cystic fibrosis patients and aquatic habitats. J. Bacteriol. 178, 85 (1996).CAS 
 Article 
 Google Scholar 
 Nigaud, Y. et al. Biofilm-induced modifications in the proteome of Pseudomonas aeruginosa planktonic cells. Biochim. Biophys. Acta – Proteins Proteom. 1804, 957–966 (2010).CAS 
 Article 
 Google Scholar 
 Von Ohle, C. et al. Real-time microsensor measurement of local metabolic activities in ex vivo dental biofilms exposed to sucrose and treated with chlorhexidine. Appl. Environ. Microbiol. 76, 2326 (2010).Article 
 CAS 
 Google Scholar 
 Nescerecka, A., Juhna, T. & Hammes, F. Identifying the underlying causes of biological instability in a full-scale drinking water supply system. Water Res. 135, 11–21 (2018).CAS 
 Article 
 Google Scholar 
 Prest, E. I., Hammes, F., Kötzsch, S., Van Loosdrecht, M. C. M. & Vrouwenvelder, J. S. A systematic approach for the assessment of bacterial growth-controlling factors linked to biological stability of drinking water in distribution systems. Water Sci. Technol. Water Supply 16, 865–880 (2016).CAS 
 Article 
 Google Scholar 
 Liang, K., Sobsey, M. & Stauber, C. E. Improving Household Drinking Water Quality: Use of Biosand Filter in Cambodia. https://scholarworks.gsu.edu/iph_facpub (2010).Fabiszewski De Aceituno, A. M., Stauber, C. E., Walters, A. R., Meza Sanchez, R. E. & Sobsey, M. D. A randomized controlled trial of the plastic-housing biosand filter and its impact on diarrheal disease in Copan, Honduras. Am. J. Trop. Med. Hyg. 86, 913–921 (2012).Article 
 Google Scholar 
 Miettinen, I. T., Vartiainen, T. & Martikainen, P. J. Phosphorus and bacterial growth in drinking water. Appl. Environ. Microbiol. 63, 3242–3245 (1997).CAS 
 Article 
 Google Scholar 
 Keinänen, M. M. et al. The microbial community structure of drinking water biofilms can be affected by phosphorus availability. Appl. Environ. Microbiol. 68, 434–439 (2002).Article 
 CAS 
 Google Scholar 
 United Nations (UN). Transforming Our World: The 2030 Agenda for Sustainable Development. in A New Era in Global Health 529–567, https://doi.org/10.1891/9780826190123.ap02 (2018).Serra, M. O. D. E. & Schnitzer, M. Extraction of humic acid by alkali and chelating resin. Can. J. Soil Sci. 52, 365–374 (1972).Article 
 Google Scholar 
 Smith, E. J., Davison, W. & Hamilton-Taylor, J. Methods for preparing synthetic freshwaters. Water Res. 36, 1286–1296 (2002).CAS 
 Article 
 Google Scholar 
 Sobsey, M. D. Managing Water in the Home: Accelerated Health Gains from Improved Water Supply Water, Sanitation and Health Department of Protection of the Human Environment World Health Organization Geneva. https://apps.who.int/iris/bitstream/handle/10665/67319/WHO_SDE_WSH_02.07.pdf?sequence=1&isAllowed=y (2002).Carratalà, A. et al. Solar disinfection of viruses in polyethylene terephthalate bottles. Appl. Environ. Microbiol. 82, 279–288 (2016).Attisani, M. Can solar technology generate clean water for developing nations? Renew. Energy Focus 17, 138–139 (2016).Chaidez, C. et al. Point-of-use Unit Based on Gravity Ultrafiltration Removes Waterborne Gastrointestinal Pathogens from Untreated Water Sources in Rural Communities. Wilderness Environ. Med. 27, 379–385 (2016).Clayton, G. E., Thorn, R. M. S. & Reynolds, D. M. Development of a novel off-grid drinking water production system integrating electrochemically activated solutions and ultrafiltration membranes. J. Water Process Eng. 30, 100480 (2017).Baig, S. A., Mahmood, Q., Nawab, B., Shafqat, M. N. & Pervez, A. Improvement of drinking water quality by using plant biomass through household biosand filter – A decentralized approach. Ecol. Eng. 37, 1842–1848 (2011). More