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    Biological manganese-dependent sulfide oxidation impacts elemental gradients in redox-stratified systems: indications from the Black Sea water column

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    Conservation agriculture based integrated crop management sustains productivity and economic profitability along with soil properties of the maize-wheat rotation

    Experimental site, location and climateFive years’ field experimentation on ICM was started in 2014–15 at the ICAR-Indian Agricultural Research Institute (28°35′ N latitude, 77°12′ E longitude, 229 m MSL), New Delhi, India. The study site comes under the ‘Trans IGPs’, being semi-arid with an average annual rainfall of 650 mm, of which ~ 80% occurs in July–September (south-west monsoon). The mean max. / min. air temperature ranges between 20-40ºC and 4-28ºC, respectively. The five years (2014–2019) weather data were recorded from the observatory adjoining to the experimental field, and presented in Supplementary Table 1. Before start of the experiment, a rainy season Sesbania was grown in 2014 to ensure the uniform fertility across the blocks. Initial soil samples (0.0–0.15 m depth) were collected in October 2014 after incorporating the Sesbania residues in soil. The soil samples were processed for the chemical analysis. The study site had a pH of 7.9 (1:2.5 soil and water ratio)68, 3.8 g kg−1 soil organic-C69, 94.1 kg ha−1 KMnO4 oxidizable N70, 97 µg g−1 soil microbial biomass carbon71, 51.3 μg PNP g−1 soil h−1 alkaline phosphatase72, 53.0 μg TPF g−1 soil d−1 dehydrogenase73, and 13.5 μg NH4-N g−1 soil h−1urease74.Description of different ICM modulesThe eight ICM modules were tested, comprising of four conventional tillage (CT)-based (ICM1-4) and four conservation agriculture (CA)-based (ICM5-8) modules, replicated thrice in a complete randomized block design with the plot size of 60 m2 (15 m × 4.5 m) (Table 4). The crop residues were completely removed in the CT-based modules (ICM1-4), while in the ICM5-8 modules, in-situ wheat (~ 3 Mg ha−1 on dry weight basis)) and maize (~ 5 Mg ha−1, on dry weight basis) residues were retained on the soil surface during all the seasons of crops cultivation (Footnote Table 4, Fig. 6a,b).Table 4 Description of integrated crop management (ICM) modules adopted in maize and wheat crops during the five yearsˈ fixed plot experimentation.Full size tableIn the ICM1-4 modules, the field preparation was carried out by sequential tillage operations, such as, deep ploughing using the disc harrow, cultivator/rotavator twice (0.15–0.20 m), followed by levelling in each season. In the ICM3-4, the raised beds of 0.70 m bed width (bed top 0.40 m and furrow 0.30 m) were formed during each cropping cycle using the tractor mounted bed planter, and simultaneously wheat sowing was done (Fig. 6c). In the case of maize, ridges (0.67 m length) were prepared using the ridge maker. In the CA-based ICM5-8 modules, the tillage operations, such as, seed and fertilizer placement were restricted to the crop row-zone in maize and wheat both. In the ICM7&8, the permanent raised beds (0.67 m mid-furrow to mid-furrow, 0.37 m wide flat tops, and 0.15 m furrow depth), were prepared (Fig. 6d). However, these beds were reshaped using the disc coulter at the end of each cropping cycle without disturbing the surface residues. The sowing was accomplished using the raised bed multi-crop planter.Cultural operations and the fertilizer applicationDuring every season, the maize (cv. PMH 1) was sown in the first week of July using 20 kg seed ha−1. The wheat (cv. HD 2967) crop was sown in the first fortnight of November using the seed-cum fertilizer drill (ICM1-2), bed planter (ICM3-4) and zero-till seed drill (ICM5-8) at 100 kg seed ha−1. The chemical fertilizers (N, P and K) were applied as per the modules described in the footnote of Table 4. At sowing, the full doses of phosphorous (P) and potassium (K) were applied using the di-ammonium phosphate (DAP) and muriate of potash (MOP), and the nitrogen (N) supplied through DAP. The remaining N was top-dressed through urea in two equal splits after the first irrigation and tasseling / silking stages in maize, and crown root initiation and tillering stages of wheat. In the modules receiving ¾ fertilizers (ICM2,4,6,8), the seeds were treated with the NPK liquid bio-fertilizer (LBFs) (diluted 250 ml formulation 2.5 L of water ha−1), and an arbuscular mycorrhiza (AMF) was broadcasted at 12 kg ha−1 as has been described by75. This LBFs had the microbial consortia of N-fixer (Azotobacter chroococcum), P (Pseudomonas) and K (Bacillus decolorationis) solubilizers, procured from the commercial biofertilizer production unit of the Microbiology Division, ICAR-Indian Agricultural Research Institute, New Delhi (Patentee: ICAR, Govt. of India). Weeds were managed by integrating the pre- and post-emergence herbicides, and their combinations along with the hand weeding-mulching, as mentioned in the concerned modules (Footnote Table 4). However, in the CA-based modules (ICM5-8), the non-selective herbicide glyphosate (1 kg ha−1) was used 10 days before the sowing. The need-based integrated insect-pests and disease management practices were followed uniformly across the modules.Soil sampling and analysisBefore start of the experiment, the soil sampling was done from 0.0–0.15 m depth. Afterwards, five random samples from each module from 0.0–0.30 m soil depth were collected at the flowering stage of 5th season wheat. These samples were taken from the three soil depths (0.0 to 0.05, 0.05–0.15 and 0.150–0.30 m) using the core sampler. The ground, air-dried soil samples, passed through a 0.2 mm sieve were used for the determination of the Walkley and Black organic carbon (SOC), as described by76. For the soil biological properties, the soil samples were processed, and stored at 5ºC for 18–24 h, then analyzed the soil microbial biomass carbon (SMBC), dehydrogenase (SDH), alkaline phosphate (SAP) and the urease (URE) activities.The soil microbial biomass carbon (SMBC)The SMBC was measured using the fumigation extraction method as proposed by71. The pre-weighed samples from the respective soil depths were fumigated with the ethanol-free chloroform for the 24 h. Separately, a non–fumigated set was also maintained. Further, 0.5 M K2SO4 (soil: extractant 1:4) was added, and kept on a reciprocal shaker for 30 min. and then filtered through a Whatman No. 42 filter paper. OC of the filtrate was measured through the dichromate digestion, followed by the back titration with 0.05 N ferrous ammonium sulphate. The SMBC was then calculated using the equation:$${text{S}}_{{{text{MBC}}}} = {text{EC }} times { 2}.{64}$$where, EC = (Corg in fumigated soil – Corg in non-fumigated soil), and expressed in µg C g−1 soil.The dehydrogenase activity (SDH)The SDH activity (μg TPF g−1 soil d−1) was assessed using the method of73. The soil sample (~ 6 g) was saturated with 1.0 ml freshly prepared 3% triphenyltetrazolium chloride (TTC), and then incubated for 24 h under the dark. Later on, the methanol was added to stop the enzyme activity, and the absorbance of the filtered aliquot was read at 485 nm.The alkaline phosphatase activity (SAP)The APA activity was estimated in 1.0 g soil saturated with 4 ml of the modified universal buffer (MUB) along with 1 ml of p-nitrophenol phosphate followed by incubation at 37 °C for 1 h. After incubation, 1 ml of 0.5 M CaCl2 and 4 mL of NaOH were added and the contents filtered through Whatman No. 1 filter paper. The amount of p-nitrophenol in the sample was determined at 400 nm72 and the enzyme activity was expressed as µg p-NP g−1 soil h−1.The urease activityUrease activity was measured using 10 g soil suspended in 2.5 ml of urea solution (0.5%). After incubating for a day at 37 °C, 50 ml of 1 M KCl solution was added. This was kept on a shaker for 30 min and the aliquot was filtered through Whatman No. 1 filter paper. To the filtrate (10 ml), 5 ml of sodium salicylate and 2 ml of 0.1% sodium dichloro-isocyanide solution were added and the green color developed was measured at 690 nm74. These values are reported as µg NH4-N g−1 soil h−1.Water application and productivityIn experimental modules, water was given through the controlled border irrigation method. The current meter was fixed in the main lined rectangular channel, and the water velocity was measured. To get the flow discharge, then multiplied with area of cross section of the channel. The following formulae were used to calculate the applied irrigation water quantity and depth3:$${text{Irrigation water applied }}left( {text{L}} right) , = {text{ F }} times {text{ t (i)}}$$$${text{Depth }}left( {{text{mm}}} right) , = {text{ L}} div {text{A}}/{ 1}000$$where, F is flow rate (m3 s−1), t is time (s) taken in each irrigation in each module and A is area (m2).The effective precipitation (EP, difference between total rainfall and the actual evapotranspiration) was calculated, and then EP was added to the irrigation water applied to calculate the total water applied in each module. Across the maize and wheat modules (ICM1-8), irrigations were given at the critical growth stages, such as, knee high and silking / tasseling (maize) and crown root formation, maximum tillering, flowering, heading / milking (wheat) stages, and after long dry spell (≥ 10-days).On the basis of the soil water depletion pattern (at the depth of 0.60 m), in each season, 3–6 irrigations were given to maize, while wheat received 5–8 irrigations per season or crop including the pre-sowing irrigation. The rainfall data were obtained from the meteorological observatory located in the adjoining field. The water productivity (kg grains ha−1 mm−1 of water) was measured as per the equation given below:$${text{Water productivity }} = {text{ economic yield }}left( {{text{kg ha}}^{{ – {1}}} } right)/{text{ total water applied }}left( {{text{mm}}} right)$$Additionally, the systems water productivity (SWP) was also estimated by adding the water productivity (WP) of both maize and wheat crops grown under the MWR.Yield measurementsIn each season, the maize and wheat crops were harvested during the months of October and April, respectively, leaving 0.75 m border rows from all the corners of each module. The crops were harvested from the net sampling area (6 m × 3 m, 18 m2) located at the center of each plot. Maize crop was harvested manually and the wheat by using the plot combine harvester. All the harvested produce was sun dried before threshing and the grain and straw / stover yields were weighed separately. The stover/straw yields were measured by subtracting the grain weight from the total biomass. To compare the total (system) productivity of the different ICM modules, the system yield was computed, taking maize as the base crop, i.e., the maize equivalent yield (MGEY) using the equation20:$${text{M}}_{{{text{GEY}}}} left( {{text{Mg ha}}^{{ – {1}}} } right) , = {text{ Ym }} + , left{ {left( {{text{Yw }} times {text{ Pw}}} right) , div {text{ Pm}}} right}$$where, Ym = maize grain yield (Mg ha−1), Yw = wheat grain yield (Mg ha−1), Pm = price of maize grain (US$ Mg−1) and Pw = price of wheat grain (US$ Mg−1).Farm economicsUnder different ICM modules, the variable production costs and economic returns were worked out based on the prevailing market prices for the respective years. The production costs included the cost of various inputs, such as, rental value of land, seeds, pesticides, LBFs / consortia, AMF, labor, and machinery; tillage / sowing operations, irrigation, mineral fertilizers, plant protection, harvesting, and threshing etc. The costs for the crops’ residues were also considered. The system total returns were computed by adding the economic worth of the individual crop, however, the net returns were the differences between the total returns to the variable production costs of the respective module. The Govt. of India’s minimum support prices (MSP) were considered for the conversion of grain yield to the economic returns (profits) during the respective years. Further, the system net returns (SNR) were worked out by summing the net income from both maize and the wheat in Indian rupees (INR), and then converted to the US$, based on the exchange rates for different years.Sustainable yield index (SYI)77,78described the SYI as a quantitative measure of the sustainability of agricultural rotation/practice. The sustainability could be interpreted using the standard deviation (σ) values, where the lower values of the σ indicate the greater sustainability and vice-versa. Total crop productivity of maize and wheat under the different ICM modules was computed based on the five years’ mean yield data. SYI was calculated using equation78.$${text{S}}_{{{text{YI}}}} = , left( {{-}{overline{text{Y}}}_{{{text{a }}{-}}} sigma_{{text{n}}} {-}_{{1}} } right) , /{text{ Y}}^{{{-}{1}}}_{{text{m}}}$$where, –ȳa is the average yield of the crops across the years under the specific management practice, σn–1 is the standard deviation and Y–1 m is the maximum yield obtained under the set of an ICM module.Statistical analysisThe GLM procedure of the SAS 9.4 (SAS Institute, 2003, Cary, NC) was used for the statistical analysis of all the data obtained from different ICM modules to analyze the variance (ANOVA) under the randomized block design79. Tukey’s honest significant difference test was employed to compare the mean effect of the treatments at p = 0.05.Authors have confirmed that all the plant studies were carried out in accordance with relevant national, international or institutional guidelines. More

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    French crop yield, area and production data for ten staple crops from 1900 to 2018 at county resolution

    Crop dataCrop area (in hectare, ha, for sown areas) and production (in kg) statistics on departmental level from 1900 until 1988 were collected from books of national agricultural statistics (‘Statistique agricole annuelle’ or ‘Annuaire de statistique agricole’) compiled by the French Ministry of Agriculture; detailed references are provided in the supplementary information. Numbers were manually digitized from photocopied versions of the original paper documents. Data from 1989 to 2018 were derived from digital statistics from the Agreste database (‘Statistique agricole annuelle’ compiled by the Service de la Statistique et de la Prospective (SSP), Secrétariat Général du Ministère de l’Agriculture, de l’Agroalimentaire et de la Forêt (MAAF), France); details are provided in the supplementary information. Yields were calculated from total production and sown area for each department to avoid apparently often incorrect yield values printed in the old statistics books. Yields are given in kilogram per hectare (kg/ha, for sown area) for dry mass with 10–16% moisture content, depending on the crop.Data are available for ten crops: soft wheat (spring and winter separately), durum wheat, maize, oats (spring and winter), rapeseed (spring and winter), barley (spring and winter), potatoes, sugarbeet, sunflower and wine. The split into spring and winter crops eventually results in 18 distinct crop-cultivar types. Time frames with available data and the correspondence between French and English names are provided in Table 1.Table 1 Data set description for yields on department level.Full size tableThe shapes of French departments have changed over time. We use the 96 mainland (Metropolitan France) departments in their current form and subsume historical values to modern departments as follows. Corsica was one single department until 1975 but then split into Corse-du-Sud and Haute-Corse. Data for Corsica until 1975 were split equally (area, production) or copied (yield) to both new departments. Seine and Seine-et-Oise were two departments until 1967, but then subdivided into seven new departments on 1 January 1968. To account for this, we consider the values of the seven new departments (Essonne, Hauts-de-Seine, Paris, Seine-Saint-Denis, Val-de-Marne, Val-d’Oise, Yvelines) only from 1968 on and unite the two old departments into one counter-factual (“Seine_SeineOise” in the data tables) until 1967.Multiple cropping per year within this set of crops is accounted for by separate area data, but is practically nonexistent in France6.Quality filtersSome yield values had to be considered as outliers, also after checking for digitizing errors. There were four criteria for defining an outlier. First, absolute yield values larger than a physiologically currently unreachable threshold were removed; threshold values were 15 t/ha for barley and durum wheat, 200 t/ha for sugarbeet and potatoes, 20 t/ha for maize, oats and wheat, 10 t/ha for rape and sunflower and 200 hl/ha for wine. These thresholds were chosen to eliminate visually obvious outliers likely due to mismatches between area and production records. The values are set slightly above current maximum attained yields, thus remaining permissive and removing only obvious errors in this first step. Additionally, all yield values for winter rape in 1944, spring rape in 1968 and spring barley in 1980 were removed due to wrongly reported values in the yearbooks. This first step removed in total 167 yield data points. Second, the top 1% of yield values across all departments per decade were removed. Third, values above or below the mean +/− four times the standard deviation of each crop-department time series (for yield, area and production separately) were removed. Fourth, and finally, a similar variance filter as in the third step was applied within each decade of a single time series, filtering values above or below decadal mean +/− two (for yield) or three (area, production) decadal standard deviations. The latter three filters removed, on average, 3.6% of the yield and 0.2% of the area or production data, respectively (Table 1). There were, as a median, 43 yield outliers per department (out of 1,260 data points on average), with a range of 4 (department Hauts de Seine) and 255 (Nord) and an interquartile range of 35–50 outliers. Outliers were masked as missing values to avoid introducing a bias from any correction. In the accompanying data sets we provide two version of the full data set, one without any corrections (“RAW”) and one where the filters described above have been applied (“FILTERED”).ValidationNationally aggregated area, production and yield data from our data set were validated with national data from 1961 to 2018 provided by the FAO (http://faostat3.fao.org/home/E). Area and production data for crops with separate spring and winter data were summed on department level to test agreement with area and production data digitized for the ‘total’ crop. More

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    Climate drives long-term change in Antarctic Silverfish along the western Antarctic Peninsula

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