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    Influence of short and long term processes on SAR11 communities in open ocean and coastal systems

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    An epidemiological model for mosquito host selection and temperature-dependent transmission of West Nile virus

    Among the 325 municipalities in Greece during the period 2010–2021, WNV events, defined as the occurrence of at least one laboratory-confirmed human WNV case during a specific year, were reported in 154 (47%) municipalities, while the remaining 171 did not report any WNV case. WNV events were reported for a period ranging from one to eight years: 54 (35%) municipalities reported laboratory-confirmed WNV cases in only one year, 38 (25%) in two years, 30 (19%) in three years, 12 (8%) in four years, 10 (6%) in five years, 6 (4%) in six years, 1 (1%) in seven years, and 3 (2%) in eight years. This means that in 60% of the positive areas (82 municipalities out of 154), WNV appeared at most for two years, in 27% (42 out of 154) between three and four years, and in the remaining 13% (20 out of 154) for five years or more. Considering the total number of reported laboratory-confirmed human WNV cases across the twelve years (Fig. 1), in approximately 50% of the positive municipalities (78 out of 154), at most 4 cases were reported: 1, 2, 3, and 4 WNV cases were reported in 24, 32, 11, and 11 municipalities, respectively. Overall, 39 municipalities recorded a number of WNV cases ranging from 5 to 10 (third quartile), 34 a number ranging from 11 to 46, while the remaining 3 municipalities recorded a number of WNV cases equal to 56, 71 and 94.Figure 1(a) Map of Greece with total numbers of laboratory-confirmed human WNV cases throughout the 12-year period 2010–2021, with breakdowns by municipality. White denotes municipalities where no human cases were observed. (b) Map of Greece with total numbers of modelled human WNV cases throughout the 12-year period 2010–2021, with breakdowns by municipality. White denotes municipalities where no human cases were modelled.Full size imageModel evaluation and comparison with MIMESISWe investigated the ability of the MIMESIS-2 model to correctly identify the occurrence of WNV events, both in space and time, and its capacity to quantify the annual number of human WNV cases and the timing of the first WNV event in the year. The performance of many quantities of interest, such as the severity and timing of occurrence of human WNV cases, was also compared output from the original MIMESIS model26.Occurrence of WNV eventsStarting with the spatial analysis, we considered the fit of the model to replicate the observed 385 WNV events out of 3,900 (325*12) possible events across municipalities. MIMESIS-2 was able to correctly identify 356 of them, generated only one false alarm, and correctly modelled 3,514 true negatives.The performance of MIMESIS-2 was then evaluated according to four indices: the probability of detection (POD), false alarm rate (FAR), miss rate (MIS), and critical success index (CSI), described in the Methods section. For the POD, MIS and CSI, we considered the 154 municipalities with at least one reported and laboratory-confirmed human WNV case over the 12-year period, while for the FAR, we considered the 153 municipalities where at least one human WNV case was modelled over the same period. We split the (0.0–1.0) index interval into five equally sized bins to derive for each index, the fraction of municipalities falling into each bin. Both the POD and CSI were above 0.8 for 139 municipalities out of 154, while the MIS was below 0.2 for 142 municipalities (out of 154) and the FAR was always below 0.2, with one false alarm produced in a municipality where WNV events were observed in eight out of twelve years (Table 1).Table 1 Capacity of the MIMESIS-2 model to correctly model laboratory-confirmed human WNV cases.Full size tableWe also analysed how the model performed in different years by studying the multiannual evolution of the indices. Both the aggregated POD and CSI were equal to 0.92, with annual variations ranging from 0.72 (2021) to 1 (2011 and 2014). The aggregated MIS was 0.08, ranging from 0.0 (2011 and 2014) to 0.28 (2021). The FAR was virtually 0, being always equal to 0.0, with the only exception being 2017, when it was 0.1 (Table 2).Table 2 Capacity of the MIMESIS-2 model to correctly model laboratory-confirmed human WNV cases by year and in the whole observed time period.Full size tableMagnitude and timing of WNV events: performance and comparison with MIMESISTo evaluate the ability of MIMESIS-2 to capture the magnitude and timing of WNV events, we first considered the discrepancy between the overall number of observed and modelled WNV cases during the 12-year period for each municipality. Out of the 153 municipalities where at least one case was modelled across the 12 years, 76 (50%) had at most 4 modelled cases of WNV: 1, 2, 3, and 4 WNV cases were modelled in 22, 31, 13, and 10 municipalities, respectively. In 42 municipalities, the number of modelled cases ranged from 5 to 10 (which, as for the observed WNV cases, coincided with the third quartile), and in 32 municipalities, the number ranged from 11 to 47, while the remaining 3 municipalities had 55, 70, and 99 modelled cases (Fig. 1).The MIMESIS-2 model closely replicated the total number of laboratory-confirmed WNV cases during the 12-year period. When considering only the 154 municipalities that recorded at least one WNV event during the considered period (excluding the true negatives), for 140 of them, the modelled number of cases fell within a ± 10% error range of the observed value, whereas for 149 the modelled number of cases fell within the ± 25% error margin. Only two municipalities showed a percent error above 50%. These were particular instances where only one WNV case was reported throughout the considered period, while MIMESIS-2 fitted zero human cases. For the original MIMESIS model, 63 and 84 municipalities fell within the ± 10% and ± 25% error margins, respectively, while 31 municipalities—mainly those where few cases were observed— had a relative error ≥ 100% (Fig. 2).Figure 2(a) For MIMESIS-2, modelled (IHMOD) vs. observed (IHOBS) human WNV cases in each municipality in the period 2010–2021. The inner black line represents the main diagonal where ideally the points would lie in case of perfect fit, while the dashed green, black and red lines represent, respectively, the ± 10%, ± 25% and ± 50% error margin. (b) Same quantities for MIMESIS. (c). Breakdown of the week of first WNV incidence by year. Plotted are the modelled quantities (WYMOD) for each of the 325 municipalities on the y-axis and the observed quantities (WYOBS) on the x-axis. The continuous line represents the main diagonal where ideally the points would lie in case of perfect fit, while the dashed lines represent the ± 4-week error margins.Full size imageTo further evaluate the bias of the model across all municipalities and years, we explored the difference between the yearly modelled and observed human WNV cases both with MIMESIS-2 and the original MIMESIS (IHMOD-IHOBS) across municipalities. In MIMESIS-2, we excluded 3,514 true negative cases to avoid distorted conclusions. For the remaining 386 cases, the mean bias was -0.04 indicating a possibly unbiased model, with the standard deviation (SD) of the residuals equal to 0.66 (original MIMESIS: mean bias 0.33, SD 2.07, after removing 3,387 true negatives) (Supplementary Fig. 1).Across the 325 municipalities and the 12 years, 385 WNV events were observed, while on 3,515 occasions, no laboratory-confirmed human WNV cases were reported; on 162 occurrences, 1 case was reported, and on 67 and 39 occasions, 2 and 3 cases were reported, respectively. The maximum yearly number of human WNV cases observed in a single municipality was 38. Considering the modelled human WNV cases with MIMESIS-2, the distribution of the 356 hits ranged between 1 and 37 modelled cases, closely mimicking the distribution of the observed cases, since 1, 2 and 3 human WNV cases were modelled on 129, 72 and 37 occasions, respectively. For the 29 misses, the observed numbers of human cases were 1 (24 times), 2 (3 times), or 3 (2 times). The only false alarm was produced in the Pellas municipality, where WNV events were observed in 8 out of the 12 years.We evaluated the timing of the first occurrence of WNV in humans for any municipality and year. Ignoring the municipalities with zero cases, the observed and MIMESIS-2-modelled first WNV cases occurred between weeks 22 and 44 and weeks 24 and 36, respectively. Modelled values tended to be dispersed around the observed ones: excluding the 3514 true negatives, 290 (75.13%) of the remaining 386 cases fell into the ± 4-week error margins from the observed cases (Fig. 2). This translated into a much lower bias of the week of first appearance (WYMOD-WYOBS) with respect to MIMESIS (Supplementary Fig. 2).Case study: The Pellas municipalityIn addition to presenting the overall performance of the model throughout different years and Greek municipalities, we highlight here the capacity of the model to capture population-specific behaviour and epidemiological features, such as the force of infection, that is, the rate at which susceptible humans, birds, and mosquitoes become infected, by presenting a single municipality case study for the municipality of Pellas. The Pellas municipality had the highest number of observed WNV cases over the 12-year period with a total of 94 human WNV cases, 38 in 2010, 16 in 2018, and 13 in 2021, no cases from 2014 to 2017, and between 4 to 8 cases in the remaining years.We considered the impact arising from the changes in parameters defining the forces of infection. In addition to the introduction of bird (({psi }_{B})) and human (({psi }_{H})) host selections, changes included modifications for the mosquito-to-bird (({p}_{M})) and bird-to-mosquito (({p}_{B})) probabilities of transmission, whose values were made temperature-dependent following Vogels et al.21, and the replacement of the mosquito-to-bird (({varphi }_{B})) and mosquito-to-human (({varphi }_{H})) ratios with their dynamic counterparts, ({N}_{M}/{N}_{B}) and ({N}_{M}/{N}_{H}), respectively (Fig. 3). We used the May–October period for the 12 years that were considered, because this is the part of the year when Culex pipiens mosquitoes are reproductively active and the majority of human WNV cases are reported. In each year of the 12-year period, ({p}_{M}) started from 0.02, reached its peak—ranging from 0.16 to 0.25—in midsummer, and then decreased to the initial values (in the original model, ({p}_{M}=0.9)). Similarly, ({p}_{B}) started from 0.28, peaked in the same time interval—with maximal values ranging from 0.51 to 0.56—and then returned to the initial values (in the original model, ({p}_{B}=0.125)). Additionally, the dynamic specifications of ({varphi }_{B}) and ({varphi }_{H}) were shown to play an important role. Whereas in MIMESIS ({varphi }_{B}=30), in MIMESIS-2 the values started at approximately 8.6 and peaked in late summer when more human WNV cases are reported, reaching values of approximately 57, before decreasing to values ranging from 31.15 to 41.60 in late October. In MIMESIS, ({varphi }_{H}) was calibrated at the municipality level, and for Pellas municipality, it was 0.0001, whereas the dynamic counterpart in MIMESIS-2 showed a temporal evolution with a shape (but different scale) similar to that of ({varphi }_{B}), starting from values of approximately 1, peaking in late summer to values of approximately 7, and then decreasing to values of approximately 4 in late October.Figure 3The temporal evolution during May to October of the (a) mosquito-to-bird probability of transmission, ({p}_{M}), (b) bird-to-mosquito probability of transmission, ({p}_{B},) (c) mosquito-to-bird ratio, ({varphi }_{B},) and (d) mosquito-to-human ratio, ({varphi }_{H},) for both MIMESIS-2 across different years and MIMESIS for each of the 12 years from 2010 to 2021. The plots refer to the simulations for the municipality of Pella.Full size imageChanges in these parameters enter into the expression for the forces of infection. It is of major practical interest to investigate how the values for the forces of infection resulting from MIMESIS-2 may vary for different values of the relative abundance of the vectors with respect to the corresponding carrying capacity and the temperature in different months (Fig. 4). As expected, all forces of infection increased with both the temperature and the relative abundance of the infectious vertebrate hosts. It is worth noting the importance of day length, as this affects the fraction of nondiapausing mosquitoes, ({delta }_{M}), and causes the forces of infection, all other things being equal, to be potentially higher in June and July than in the other months. However, in these two months, the modelled forces of infection tend to be smaller than those in August due to the lower abundance of infectious hosts.Figure 4Contour plots of the forces of infection for May to September for different values of the relative abundance of infected hosts/vectors with respect to the carrying capacity and the temperature. All the other quantities were fixed to the amounts obtained in the simulations for Pellas municipality for 2021 at the end of the corresponding month. (a) Bird-to-mosquito force of infection (({lambda }_{BM})) as a function of the relative abundance of infected birds (({I}_{B})) with respect to the bird carrying capacity (({K}_{B})) and temperature. (b) Mosquito-to-bird force of infection (({lambda }_{MB})) as a function of the relative abundance of infected mosquitoes (({I}_{M})) with respect to the mosquito carrying capacity (({K}_{M})) and temperature. (c) Mosquito-to-human force of infection (({lambda }_{MH})) as a function of the relative abundance of infected mosquitoes (({I}_{M})) with respect to the mosquito carrying capacity (({K}_{M})) and temperature. The ranges for ({I}_{B}/{K}_{B}) and ({I}_{M}/{K}_{M}) were fixed, increasing the maximum modelled value by 20% for the considered period, while the range for the temperature was chosen considering that in the period of interest, the average daily temperature ranged from 16.6 to 27.1 degrees Celsius. Black crosses represent the modelled values for 2021.Full size imageThe bird-to-mosquito force of infection, ({uplambda }_{BM}), took values on the order of 10–4, with possible peaks of approximately 7 × 10–4 in the case of high temperature and high prevalence of birds in June and July, which were nevertheless not reached due to a low abundance of infected birds in that period. Considering the months of July and August 2021 for illustrative purposes, the resulting modelled values were 1.20 × 10–4 and 2.19 × 10–4, respectively, with the increase in August explained by a higher abundance of infected birds in that period. It is worth noting that if the infection across birds had a lead period of two weeks, the resulting ({uplambda }_{BM}) in July would become 3.91 × 10–4 (+ 226%), while an increase in the average temperature in August by 1 °C would result in ({uplambda }_{BM})= 2.36 × 10–4 (+ 8%). The mosquito-to-bird, ({uplambda }_{MB}), and mosquito-to-human, ({uplambda }_{MH}), forces of infection showed similar qualitative behaviours, albeit at different scales, and in this case, they were higher in August due to a higher prevalence of infected Culex mosquitoes in that month. More specifically, ({uplambda }_{MB}) equalled 1.06 × 10–3 and 1.22 × 10–3 at the end of July and August, respectively, and an expected two weeks for the infection of mosquitoes would result in ({uplambda }_{MB})=4.15 × 10–3 (+ 292%) at the end of July, while an increase in the average temperature in August by 1 °C would result in ({uplambda }_{MB})= 1.31 × 10–3 (+ 7%) at the end of August. Finally, ({uplambda }_{MH})=2.86 × 10–6 at the end of July, while ({uplambda }_{MH})=3.26 × 10–6 at the end of August, with the anticipation of the infection among mosquitoes by two weeks resulting in ({uplambda }_{MH})=1.12 × 10–5 (+ 290%) and an increase in the average August temperature by 1 °C leading to ({uplambda }_{MH})=3.51 × 10–6 (+ 8%). It is worth recalling that since we calibrated the model on the number of reported laboratory-confirmed human WNV cases, ({uplambda }_{MH}) represents the rate at which susceptible humans contract infection and become symptomatic leading to a recorded human WNV case.We explored changes in the populations of infectious hosts and the total population number for both mosquitoes and birds over 2010–2021 for the period spanning from May to October (Figs. 5 and 6). The population of infected mosquitoes (({I}_{M})) was initialised by calibration (see the Methods section). Each year, after a short period in which the population of infected mosquitoes slightly decreased due to a very small number of infectious birds (({I}_{B})) that prevented the infection from spreading, it started growing substantially during summer, reaching its peak in late summer, coinciding with the period when most human cases were recorded. The observed increase in ({I}_{M}) was combined with the growth ({I}_{B}) at approximately the same time (with a slightly anticipated peak), which had an amplification effect on the spread of the infection. Both ({I}_{M}) and ({I}_{B}) showed significant yearly variation, with higher modelled numbers in years where more human WNV cases were reported. The modelled total population of mosquitoes (({N}_{M})) did not show significant interannual variability, always peaking in late summer. Finally, the overall population of birds (({N}_{B})) did not show any variability in the first part of the year, when an increase due to immigration and offspring generation was observed, whereas it had a moderate interannual variability in the second half of the year. These differences may be due to heterogeneous numbers of observed infected, dead and immune birds.Figure 5The temporal evolution during May to October of (a) the number of infected mosquitoes modelled by MIMESIS-2 (({I}_{M})), (b) the total number of mosquitoes modelled by MIMESIS-2 (({N}_{M}))(,) (c) the number of infected birds modelled by MIMESIS-2 (({I}_{B}))(,) and (d) the total number of birds modelled by MIMESIS-2 (({N}_{B})) for each of the 12 years from 2010 to 2021. The plots refer to the simulations for the municipality of Pella.Full size imageFigure 6The temporal evolution during May to October of (a) the ratio between the number of WNV-infected mosquitoes modelled by MIMESIS-2 (({I}_{M,MIM-2})) and the ratio modelled by MIMESIS (({I}_{M,MIM})), (b) the ratio between the total number of mosquitoes modelled by MIMESIS-2 (({N}_{M,MIM-2})) and the ratio modelled by MIMESIS (({N}_{M,MIM}))(,) (c) the ratio between the number of infected birds modelled by MIMESIS-2 (({I}_{B,MIM-2})) and the ratio modelled by MIMESIS (({I}_{B,MIM}))(,) and (d) the ratio between the total number of birds modelled by MIMESIS-2 (({N}_{B,MIM-2})) and the ratio modelled by MIMESIS (({N}_{B,MIM})) for each of the years from 2010 to 2021. The plots refer to the simulations for the municipality of Pella.Full size imageComparison of these population dynamics with those of MIMESIS revealed interesting patterns (Fig. 6). Considering the relative number of mosquitoes in MIMESIS-2 with respect to MIMESIS, the populations in MIMESIS tended to grow faster due to a higher mosquito carrying capacity (({K}_{M})) in the original model (({K}_{M}) ≈ 8.3 × 105 in MIMESIS versus ({K}_{M}) ≈ 2.4 × 105 in MIMESIS-2), resulting in a decrease in the ratio between the amounts modelled by MIMESIS-2 and the ones modelled by MIMESIS. Significant interannual variability could be seen in the first part of the year for infectious mosquitoes, where different initial calibration values played an important role. For the populations of birds, until midsummer, the overall number modelled by MIMESIS-2 tended to be approximately 1/4 that of MIMESIS, while as of July, different patterns were observed due to the higher mortality of birds in the original MIMESIS model. In years with higher virus spread, higher mortality was reflected in a sharper decrease in bird populations; therefore, the ratio between the population modelled by MIMESIS-2 and that modelled by MIMESIS increased up to approximately 0.6 (2010). More

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    Growth response and mycoremediation of heavy metals by fungus Pleurotus sp.

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    The potential distribution of Bacillus anthracis suitability across Uganda using INLA

    Study settingUganda covers 241,037 km2 (4° N, 2° S, 29° E, 35° E) and averages 1100 m above sea level25. Uganda has a total population of 34.6 million people, with 7.4 and 27.2 million living in urban and rural areas, respectively26. It borders Lake Victoria and has an equatorial climate. The study area is mainly plateau, with a few mountains. About 20 percent of Uganda’s area is covered by swamps and water bodies, including the four Great Lakes of East Africa (Lake Edward, Lake Victoria, Lake Albert, and Lake Kyoga). The country has ten national parks housing a high diversity of wildlife and endangered species26. By 2009, agriculture ranked as the second leading contributor to the country’s Gross Domestic Product27.Surveillance data of Bacillus anthracis infectionSurveillance data of livestock and human cases from 2018 were provided by the Ministry of Health (MOH) in Uganda through the Field Epidemiology and Laboratory Training Program and the Uganda Ministry of Agriculture, Animal Industry and Fisheries (MAAIF). Geographical coordinates of anthrax cases among wildlife (from 2004 to 2010) in Queen Elizabeth National Park were obtained from a recently published study4. These cases were mapped to show the distribution of anthrax across Uganda (Fig. 1). It is only recently that Uganda has mandated systematic anthrax surveillance across the country following the outbreak that started in 2018. GPS coordinates gathered by field personnel during outbreak responses were used to map outbreak locations. The human anthrax cases were defined based on the CDC’s clinical criteria (signs and symptoms), presumptive laboratory diagnosis (Gram staining), and confirmatory laboratory diagnosis (bacterial culture, immunohistochemistry, ELISA, and PCR)28. The animal anthrax cases were also defined based on the clinical presentation, presumptive laboratory diagnosis, and confirmatory laboratory diagnosis. All cases were classified as either ‘probable’ or ‘confirmed,’ with probable defined as cases that met both the clinical and presumptive laboratory diagnostic criteria and confirmed defined as cases that met the clinical and confirmatory laboratory diagnostic criteria. A total of 497 livestock (n = 171), humans (n = 32), and wildlife cases (n = 294), both confirmed (n = 32) and probable (n = 465), occurring from 2004 to 2018 were compiled. All methods were performed in accordance with the relevant guidelines and regulations.Figure 1Distribution of anthrax presence and pseudo-absence locations across Uganda. The navy-blue circles show wildlife cases (n = 294) used for model training, the blue triangles show livestock cases (n = 171), and the red diamonds represent human cases (n = 32). The blue polygons show the locations of the 50 km, 75 km, and 100 km buffers which were constructed around the wildlife cases with a distance of 10 km between the buffers and the presence locations. The pink dots show the pseudo-absence points selected within the 50 km buffer, the orange dots show the pseudo-absence points selected within the 75 km buffer, and the white dots show the pseudo-absence points selected within the 100 km buffer. Prediction maps were developed using the Quantum Geographic Information System software (QGIS). URL: https://qgis.osgeo.org (2020).Full size imageEnvironmental variable processingCorrelative studies of environmental risk factors for anthrax outbreaks suggest that temperature6,29,30,31,32,33,34,35,36,37, precipitation6,29,30,31,32,33,34,35,36,37,38,39, elevation6,29,31,32,34,35,37,38,39, soil (type, calcium concentration, pH, carbon content, and moisture)6,30,31,33,34,36,37,39,40,41,42,43, vegetation6,29,31,34,36,37,38,39,40,42,43, and hydrology37,44 are some of the major drivers of B. anthracis suitability. A total of 26 environmental predictors (Fig. 2) were selected for this study based on these known variables. These comprised 19 bioclimatic variables (the mean for the years 1970–2000) collected from the WorldClim database version 2 (https://www.worldclim.org/data/worldclim21.html) at a resolution of 30 s (~ 1 km2)45. Four soil variables, including exchangeable calcium at a depth of 0–20 cm, soil water availability, soil pH (10×) in H2O at a depth of 0 cm, and soil organic carbon at a depth of 0–5 cm, were retrieved from the International Soil Reference and Information Centre (ISRIC) data hub at a resolution of 250 m (https://data.isric.org/geonetwork/srv/eng/catalog.search#/home). Distance to permanent water bodies was derived from a global hydrology map provided by ArcGIS online version 10.6.146. Elevation data of 1 km2 in resolution was obtained from the Global Multi-resolution Terrain Elevation Data (GMTED2010) dataset available from the United States Geological Service. Finally, the monthly Enhanced Vegetation Index (EVI) data for the years 2004, 2005, and 2010 (36 tiles in total) were obtained from The Aqua Moderate Resolution Imaging Spectroradiometer (MODIS) Vegetation Indices (MYD13A3 v.6) at a spatial resolution of 1 km (https://lpdaac.usgs.gov/products/myd13a3v006/). The single variable, mean EVI, was calculated in QGIS by averaging all 36 tiles. The EVI minimizes variations in the canopy background and maintains precision over conditions with dense vegetation.Figure 2Results of correlation between covariates using Pearson’s correlation test. Correlation between covariates was shown by red numbers (negative correlation) and blue numbers (positive correlation). BIO1 = Annual Mean Temperature, BIO2 = Mean Diurnal Range, BIO3 = Isothermality, BIO4 = Temperature Seasonality, BIO5 = Max Temperature of Warmest Month, BIO6 = Min Temperature of Coldest Month, BIO7 = Temperature Annual Range, BIO8 = Mean Temperature of Wettest Quarter, BIO9 = Mean Temperature of Driest Quarter, BIO10 = Mean Temperature of Warmest Quarter, BIO11 = Mean Temperature of Coldest Quarter, BIO12 = Annual Precipitation, BIO13 = Precipitation of Wettest Month, BIO14 = Precipitation of Driest Month, BIO15 = Precipitation Seasonality, BIO16 = Precipitation of Wettest Quarter, BIO17 = Precipitation of Driest Quarter, BIO18 = Precipitation of Warmest Quarter, BIO19 = Precipitation of Coldest Quarter.Full size imageAll environmental variables were resampled using the R package ‘Resample’47 to a resolution of 1 km and clipped to the extent of Uganda. Since data sampling for wildlife data (used in model training) was not done systematically across the study area, target backgrounds buffers (polygon buffers created at certain radii from the training points and used for the random selection of pseudo-absences) were created for model calibration to reduce sampling bias. As there was no information on the sampling radius, a sensitivity analysis was conducted by creating target backgrounds using circular buffers of radius 50 km, 75 km, and 100 km around the presence points used for model training, leaving 10 km between the presence points and the various buffers (Fig. 1). Quantum Global Information System (QGIS) version 3.16 (https://qgis.org) software was then used to add 294 random pseudo-absence points within each buffer polygon giving a ratio of 1:1 for the training presences to pseudo-absences. A recent study explored how four approaches of pseudo-absence creation affect the performance of models across different species and three model types by building both terrestrial and marine models using boosted regression trees, generalised additive mixed models, and generalised linear mixed models48. They then tested four methods for generating pseudoabsences across all the different model types: (1) correlated random walks (RW); (2) reverse correlated RW; (3) sampling pseudoabsences within a buffer area surrounding the presence points; (4) background sampling48. The findings of the study suggested that the separation or distance in the environmental space between the presence locations and the pseudoabsences was the most significant driver of the model predictive ability and explanatory power, and thus finding was consistent across the three model types (boosted regression trees, generalised additive mixed models, and generalised linear mixed models) and both the terrestrial and marine habitats48.The values of the environmental variables were then extracted for each presence and pseudo-absence location using the raster package in R. With these, we did an initial data exploration to check for outliers within the covariates, collinearity, and to explore the relationships between the covariates and the response variables (presence or absence of anthrax). Cleveland dot plots were used to check for possible outliers. Following the outlier checks, variance inflation factors (VIF), pairwise plots, and Pearson correlation coefficients with correction for multiple comparisons were used to measure the statistically significant correlation between the covariates (Fig. 2). For variables that were highly correlated (correlation greater than 0.6) or those with high variance inflation (VIF  > 3), only one was used in the modelling process. Five variables were selected following this analysis: Temperature seasonality (BIO4), elevation, distance to water, soil calcium, and soil water (Table 1).Table 1 Summary of the environmental variables used.Full size tableModelling anthrax suitability across UgandaQGIS v.3.16 (https://qgis.org) and the R statistical package version 4.1.049 were used to conduct data visualization, cleaning, and model analysis (R code used available in a Github repository: https://github.com/valentinandolo/Uganda-Spatial_Model/tree/master). The wildlife cases (n = 294) were used for model training and testing. The remaining human (n = 32) and livestock (n = 171) cases were used for model evaluation. Since the wildlife case locations were recorded from 2004 to 2010, while the livestock and human cases were recorded in 2018, the latter locations were both spatially and temporally distinct from the wildlife cases, making an excellent basis for block cross-validation of the final model performance50. Random partitioning of the data into training and testing sets can inflate the performance of a model and underestimate the error in the spatial prediction evaluation50. Block cross-validation uses spatial blocks that separate the testing and training datasets; thus, the method has been suggested to be a good technique for error estimation and a robust approach for measuring a model’s predictive performance50.The INLA package in R was applied to model the suitability of B. anthracis across Uganda. INLA calculates the spatial interaction effects using a Stochastic Partial Differential Equation (SPDE) method, which estimates a continuous Gaussian Markov Random Field (GMRF) where the correlation between two locations in space is specified by the Matérn correlation which is explained in more detail elsewhere51. The initial step in fitting an SPDE model is the creation of a Constrained Refined Delaunay Triangulation or a mesh to illustrate the spatial process51. R-INLA uses a function called ‘inla.mesh.2d()’ that applies a variety of arguments to build the mesh, these include: loc, loc.domain, boundary, max.edge, and cutoff51. The loc argument contains the point locations which provide information about the area of study and are used to create the triangulation nodes. Alternatively, a polygon of the study area can be used to identify the extent of the domain via loc.domain. We applied the point locations using the loc argument. We then specified the boundary of the mesh as a convex hull. We used the max.edge argument to specify the maximum edge length for the inner mesh domain/triangles and the outer triangles. We did this by first studying the distribution of distances between the point locations for the training presences and pseudo-absences. Most points were within a distance of about 90–100 km away from each other, thus, a possible guess for the range at which spatial autocorrelation persists was 100 km. We used a distance of 20 km as a range guess to create a finer mesh which has been shown to produce more precise models. We specified the maximum edge length for the inner triangles as 20 km divided by 5 (4 km) and the maximum edge length for the outer triangles as 20 km. Finally, the cutoff argument sets the minimum distance allowed between point locations. We divided the maximum edge length for the inner triangles by 5 (4 km divided by 5 = 0.8 km), meaning that points that were closer in distance than 0.8 km were replaced by one vertex to avoid the occurrence of small triangles.The spatial effect, which is a numeric vector, then links each observation within the data to a spatial location, thus, accounting for region-specific variation that cannot be accounted for by the covariates. Following the recommendation by Lindgren and Rue52, multivariate Gaussian distributions with means of zero and a spatially defined covariance matrix were used to model the spatial effect. Several versions of Bayesian hierarchical additive models were created by estimating a Bernoulli generalized additive model (GAM) with and without spatially correlated random effects. The Bernoulli GAM is defined as shown in Eqs. (1) and (2)$${C}_{i} sim Bernoulli left({p}_{i}right),$$
    (1)
    $$logit left({p}_{i}right)= alpha +sum_{j=1}^{m}{beta }_{j}left({X}_{j,i}right)+ sum_{k=1}^{l}{f}_{k}left({X}_{k,i}right)+ {mu }_{i},$$
    (2)
    where ({C}_{i}) denotes the observed value, such that: B. anthracis presence or absence at a given location i (i = 1, …, n; n = 588) is given as ({C}_{i}), where ({C}_{i}) =1 if B. anthracis was present, and ({C}_{i})=0 if absent. Logit is the link function for binomial family, ({p}_{i}) is the expected value of the response variable (the probability of B. anthracis suitability) at location i, α is the intercept, ({X}_{j,i}) and ({X}_{k,i}) are the j th and the k th covariates at a location i, ({beta }_{j}) are the beta coefficients, ({f}_{k}) are the smooth functions (cubic regression splines) for k th covariates, and ({mu }_{i}) is the spatial random effect at the location i53. The number of variables in linear term (m) and the non-liner term (l) are different because the variables employed in each term are different. We estimated both linear and non-linear effects for the covariates. Our overall database had 294 B. anthracis pseudo-absences generated randomly across the target background buffers to match the number of species presences recorded. Because we had no prior information, a Gaussian prior distribution with a mean of zero (default no effect prior unless data is informative) was applied for all the model parameters. The posterior mean, standard deviation, and 95% credible intervals were estimated for all the parameters.Model selectionSeveral different candidate models were examined. First, a baseline model was built using only the intercept. A second baseline model was then built, which included the intercept and spatial random effects only. Covariates were added to the second baseline model (intercept plus spatial effects model) without any smoothing function (i.e., only linear effects). The contribution of the spatial random effect was then re-examined by taking it out from the model. Smoothing functions were then added to all covariates, and the same procedure was repeated. Model selection was done using this forward stepwise approach. The final model was run using the three different target background buffers to identify the buffer distance with the lowest Deviance Information Criterion (DIC). The various options were assessed using the DIC54, Watanabe-Akaike information criterion (WAIC), and the Conditional Predictive Ordinate (CPO). The DIC and WAIC were chosen because they are commonly used to assess model performance by measuring the compromise between the goodness of fit and complexity. For CPO, the logarithmic score (− mean(log (CPO)) (LCPO) was calculated and used55. CPO can also be used to conduct internal cross-validation of models using a leave-one-out approach to evaluate the predictive performance of the model. Lower LCPO, DIC, and WAIC estimates suggest superior model performance. Thus, the favoured model had the lowest values across the 3 metrics.Model validation and evaluationModel validation for the favoured model was conducted using an independent evaluation dataset comprising of livestock and human outbreaks occurring at different spatial locations and 8 years after the training data. The omission rate, which indicates the proportion of positive test locations that end up in pixels predicted to be unsuitable for B. anthracis56, was used to validate the model. A low omission rate indicates good model performance. The threshold for suitability was the probability threshold that maximized the sensitivity and specificity of the model. The sensitivity was then derived by calculating the proportion of positive test locations that end up in pixels predicted to be suitable for B. anthracis56. A high sensitivity indicates good model performance.Model predictionThe favoured model selected using the criteria described above was used to generate countrywide prediction maps showing the posterior mean values, standard deviation, and the 95% credible intervals of the probability of B. anthracis suitability. The raster package in R was used to make the prediction maps. Bayesian kriging was done by treating all model parameters as random variables to include uncertainty in the prediction57. This kriging is built into the INLA framework via the SPDE, which allows a Delaunay triangulation to be constructed around the presence and absence locations within the sampling frame52. INLA then conducts model inference and prediction at the same time by considering the prediction points as locations missing the response variable (set to NA)51. Following successful model prediction, additional linear interpolation functions then generate the output for the whole study area scaled from 0 to 1.Ethical approvalEthical approval for this study was provided by the Human Biology Research Ethics Committee, University of Cambridge, UK (Ref: HBREC.2019.02) the School of Veterinary Medicine and Animal Resources Institutional Animal Care and Use Committee, Makerere University, Uganda (Ref: SVAREC/21/2019); and the Uganda National Council for Science and Technology. Informed consent was obtained from all subjects and/or their legal guardian(s). All methods were performed in accordance with the relevant guidelines and regulations. More

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    Tube length of chironomid larvae as an indicator for dissolved oxygen in water bodies

    Chironomids have the ability to survive and reproduce in polluted environments, and thus they are included in many ecological studies where approaches may be taxonomic or functional16. The diversity of most macroinvertebrates is controlled by the oxygen level of water, but chironomids may survive in hypoxic conditions where the oxygen concentration may be less than 3 mg l−117. The current study demonstrates that changing seasons, as well as anthropogenic activities, have a significant impact on the levels of DO in aquatic bodies. As observed from the result, DO highly influences the tube length of the chironomid larvae. Since KWC is a wastewater canal, the average oxygen level is lower (5.24 ± 1.14 mg l−1) than KFP (6.63 ± 1.28 mg l−1) which is a normal fish culturing pond. It has also been observed that the average tube length of the chironomid larvae of KWC (8.66 ± 0.88 mm) is higher than KFP (7.68 ± 0.62 mm), which indicates that a low concentration of DO promotes the building of longer tubes in natural conditions. Similar observations were also observed in laboratory conditions. When the oxygen level (7.03 ± 0.41 mg l−1) in the experiment was kept in the normal range, there was negligible variation in tube length (7.61 ± 0.31 mm). But when the concentration of oxygen is gradually reduced by dilution, the tube length starts to increase accordingly, which is explained graphically in Fig. 4. The regression model of both the experimental conditions also supports the hypothesis that the tube length has an inverse relationship with DO. The scatter plot and simple linear regression confirmed the inverse relationship between DO and tube length (Figs. 1 and 2).Chironomid larvae are able to grow in the polluted water of a wastewater pond as dominant macroinvertebrates18. It is observed that those larvae living in the sand tubes are more susceptible to chemical pollutants than the larvae living in silt tubes7. Sand particles are bigger than silt and are not suitable for the survival of larvae19. Chironomus riparius larvae make their tubes from different external particles and their own proteins20. Midge larvae are the inhabitants of sediments, and at the same time, sediment is the depository of different inorganic, organic, and heavy metals. In such cases, the tube of chironomid larvae may act as a defensive structure, which protects them from the adverse effects of undesirable pollutants and may increase their tolerance against such chemicals21,22,23.Larvae can thrive in benthic sediments with high decaying organic content and very low DO concentrations in water bodies24. In poor DO concentration, larvae can survive due to the presence of haemoglobin in their body tissue fluid, which plays an important physiological role in increasing respiratory efficiency, as was observed in Chironomus plumosus. Longer tube length may help larvae generate better respiratory currents so that they can cope with a low DO environment.Tube length is crucial for living in water because primarily tubes protect them from outer environmental factors like predators, and pollution. It was observed during this study that when the DO of water is low, larvae make elongated tubes to reach the upper layer of water, where the DO level is comparatively high. To get their required amount of oxygen, the larvae increase the tube length towards the water surface and increase the DO in tube water by undulating the body and other structures, creating a current inside the tube25,26. On contrary, when the DO level of the surrounding water of chironomid is sufficient, they can manage their normal physiological activities with the available oxygen. They need not to elongate their tube length. That’s why their tube length is inversely related to the DO of their surrounding medium.If tube length does not increase in size in hypoxic water, larvae will not be able to meet their oxygen demand. If the DO of water decreases, tube length will increase and vice versa. Behavioural and physiological adaptations of chironomids larvae make them successful to live in a hypoxic environment. Thus, in hypoxic conditions, larvae with longer tubes are able to gather more oxygen from the upper layer of water and get more space to create a current of water to increase the amount of O2 inside the tube by undulating the preanal papillae, anal gill, ventral gills. This would explain why the tube length of chironomids depends on the DO of water. Hence by measuring the tube length with a standard measuring scale, one may get an idea about the quality of water, especially DO, before doing any chemical analysis. The work seems to be unique and novel for its own kind. More

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    Defensive functions and potential ecological conflicts of floral stickiness

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    Global crop yields can be lifted by timely adaptation of growing periods to climate change

    Rule-based mean sowing and maturity datesLocation- and climate-specific mean crop calendars are computed by combining two rule-based approaches published by19 and22 to simulate sowing and physiological maturity dates of grain crops, respectively. The assumption is that farmers select growing seasons based on the mean climatic characteristics of their specific location and on the physiological limitations (base and optimum temperatures for reproductive growth; sensitivity to terminal water stress) of the respective crop species. Accordingly, they select sowing dates and cultivars with phenologies that, on average, meet these adapted maturity dates.The climate is classified into (i) seasonality types, based on the coefficient of variation of monthly mean temperature and precipitation and (ii) temperature levels, based on the temperature of the warmest month as compared to the base and the optimum temperatures for the crop reproductive growth. Optimal temperatures for sowing, optimal temperature ranges for grain filling, as well as indicators of soil moisture conditions (based on precipitation/potential-evapotranspiration ratio (P/PET)), are defined as global parameters for each crop (Supplementary Table 1) and used as thresholds to identify the best timing for sowing and for the start or end of the crop grain-filling phase. To cope with fluctuations of daily values around these thresholds, mean daily temperature, precipitation and potential evapotranspiration are derived by linear interpolation between monthly values.We distinguish between spring and winter crop types. Maize, rice, sorghum, and soybean are simulated as spring crops only, for wheat we simulate both types. For spring crops, farmers sow the crops at the onset of the wet season (first day of the wettest 120 consecutive days), in case of prevailing precipitation seasonality, or on the day of the year when temperatures increase above crop-specific temperature threshold19 (Supplementary Table 1), in case of temperature-driven seasonality.For wheat, we distinguish three types: winter wheat with vernalization is chosen if monthly temperatures fall below 0 °C, but winter is neither too harsh (temperature of the coldest month is higher than −10 °C), nor too long (temperatures fall below the sowing temperature threshold (12 °C) after 15th September (North hemisphere) or 31st March (South hemisphere)19). Winter wheat without vernalization is grown if winters are mild (the temperature of the coldest month is higher than 0 °C) without dormancy. In this case, wheat is sown 75 days before the coldest month of the year. This rule was arbitrarily chosen based on observed wheat sowing dates in mild winter regions. If the conditions for growing any of the winter-wheat types are not met (winter too harsh and too long), then spring wheat (without vernalization) is chosen. Note that the computed sowing dates do not differ between rainfed and irrigated for any of the crops.The mean maturity date is chosen so that the crop grain-filling phase, the most critical for yield formation, occurs under the least stressful conditions possible in that location and climate as follows. Under precipitation seasonality, grain filling starts towards the end of the rainy season, when a P/PET threshold is crossed. Under temperature seasonality, (a) grain filling of spring crops starts in the warmest month of the year (if summer temperatures are optimal), or right after temperatures return within an optimal range; (b) grain filling of winter crops ends in the warmest month of the year (if summer temperatures are optimal), or right before temperatures exceed the optimal range; (c) eventually, maturity is advanced to escape terminal water stress. Note that the grain-filling phase has a static duration of 60 days for maize and 40 days for all the other crops. This assumption is based on empirical relationships between the total growth period and the post-flowering reproductive phase, showing that the partition between the vegetative and reproductive phase of grain crops follows a saturation curve that levels off after 90–100 days of total growth duration54. Different crops are assumed to have only one crop cycle (sowing-to-maturity) per year, therefore neither multi-cropping systems nor crop rotations are accounted for in the decision-making rules. A detailed description of the rules and parameterization can be found in refs. 19, 22.Simulated crop calendars reflect current farmers’ managementSimulated historical crop calendars, driven by the bias-corrected climate dataset WFDEI23, largely agree with observations11,12,13. We compare results both at the country and grid-cell level because, although the observed crop calendars used here are gridded datasets, their underlying sources are often reported per country. The country-level comparison highlights that the agreement is good for most countries, importantly, including those with large cropland area. The area-weighted Mean Absolute Error (MAE) is close or well below 30 days for all considered crops (Fig. 4). The simulated crop calendars compare well with the observed data also at the grid-cell level. Large areas, including major agricultural regions of importance for global yields, show deviations within ±15 days for both sowing and maturity dates (Supplementary Table 2 and Supplementary Figs. 21–24). However, evaluating the accuracy below 30 days is limited by the time resolution of the observations, which is either (i) monthly11 and converted by us into daily values, by taking the mid-day of the reported month, or (ii) daily12,13, but resulting from averages over large time windows (often  > 1 month). Overall, the accuracy of the model is in line with the original evaluations of this rule-base method19,22, as well as with other studies simulating average growing periods across large regions18,20.Fig. 4: Evaluation of simulated crop calendars.Country-level comparison of simulated and observed sowing (A) and maturity (B) dates (day of the year) for five crops. Each circle refers to a country and a crop, the size of the circle is scaled according to the cropland area per country. The area-weighted Mean Absolute Error (MAE, days) is reported for each crop. Crop-calendar simulations are based on WFDEI reanalysis climate forcing23 for the period 1979–2012. The observed crop calendar includes different sources11,12,13.Full size imageSimulation of daily crop phenology and yields with the LPJmL crop modelWe perform a modeling experiment across the global land grid at 0.5° × 0.5° resolution. We used the LPJmL5 crop model24,25 to simulate daily growth and phenological development of five crops, driven by climate projections from four General Circulation Models (GCMs) GFDL-ESM2M, HadGEM2-ES, IPSL-CM5A-LR and MIROC5 under the Representative Concentration Pathways 6.0 (RCP6.0) as provided in bias-adjusted form from the CMIP5 archive by the ISIMIP2b project42. Irrigated and rainfed production systems are simulated separately on their current harvested areas11, which is also used to compute total crop yields at grid-cell and global scale, as the product of yield by crop-specific area. A first 5000-year spin-up simulation is used to initialize all model pools (e.g., soil carbon and nitrogen content). A second spin-up simulation of 390 years is used to introduced effects of historical human-driven land-use change on these pools. A change in cropping area for the future scenarios is not considered in this study.Phenological development is simulated based on the thermal-time model, including the effect of vernalization. All crops are assumed to be insensitive to photoperiod, due to a lack of parameters for multiple-crops and global-scale simulations. Previous global studies15,18 that have focused on maize and wheat only, have found lower performances in the growing-period simulations when using a photo-thermal model, compared to a temperature-only driven approach and thus recommend caution when using the photoperiodic response. State-of-the-art global crop models13,16 also typically do not consider sensitivity to photoperiod or assume that the photoperiodic response of the cultivars chosen in each location are perfectly tuned to the given conditions.Sowing dates are prescribed based on the external rule-based algorithm. Crop cultivars are parametrized based on the phenological units required to reach the corresponding maturity dates (TUreq, °C days). In line with15, TUreq are derived consistently with the phenological module of the crop model LPJmL for each grid cell, crop, and rule-based computed growing period from the respective climate input. They are calculated as the sum of daily mean air temperature increments above a crop-specific base temperature (TU) (Supplementary Table 1) between rule-based sowing and maturity. In addition, winter-wheat cultivars require effective vernalization days (VUreq), that range between 0 (mild winters) and 70 (cold winters), depending on the temperature of the 5 coldest months (Eq. (1))15,18.$${{{{{mathrm{V}}}}}}{{{{{{mathrm{U}}}}}}}_{{{{{{{mathrm{req}}}}}}}}=frac{70}{5}times left(1-frac{{T}_{m}-3}{10-3}right)$$
    (1)
    where Tm is the mean temperature of the month.From the day of sowing, effective TU for phenological development are accumulated daily, as the difference between the mean air temperature on that day and the crop-specific base temperature for phenological development (Eq. (2)). The vernalization effectiveness is computed daily by a scaling factor (0–1), which is then multiplied to the TU (Eq. (2)). For crops that are insensitive to vernalization, VUd is set equal one.$${{{{{mathrm{T}}}}}}{{{{{{mathrm{U}}}}}}}_{{{{{{{mathrm{req}}}}}}}}=mathop{sum }_{d=1}^{{ndays}}left({max }left(0,{T}_{d}-{T}_{{base}}right)times mathop{sum }_{0}^{d}{{{{{mathrm{V}}}}}}{{{{{{mathrm{U}}}}}}}_{d}right)$$
    (2)
    where the scaling factor VUd is computed by a three-stage linear response function with a range of optimal temperatures (Eq. 3). Temperature for effective vernalization range between −4 °C and +17 °C, with an optimum range between 3 °C and 10 °C.$${{{{{{{mathrm{VU}}}}}}}}_{d}=left{begin{array}{cc}left({T}_{d}-left(-4right)right)/left(3-10right) & {{{{{{mathrm{if}}}}}}}-4 , < ,{T}_{d} , < , 3\ 1 & {{{{{{mathrm{if}}}}}}};3,le ,{T}_{d},le, 10\ left(17-{T}_{d}right)/left(17-10right) & {{{{{{mathrm{if}}}}}}};10 , < ,{T}_{d} , < , 17\ 0 & {{{{{{mathrm{otherwise}}}}}}}end{array}right}$$ (3) In this study, we have removed the effect of vernalization on slowing down TU accumulation until 10% of the total vernalization requirements is reached. In this way, the crop can accumulate both vernalization units and heat units in fall, so that there is some leaf growth before winter (in LPJmL, the LAI curve depends on accumulated heat units).The LPJmL model simulates phenology as one single phase from emergence to maturity. Although the flowering stage is not simulated as an explicit break point, the fraction of above-ground biomass that is allocated to the storage organs (fHI) depends on the phenological progress (fTUreq, fraction of TUreq that have been fulfilled), with the bulk of the storage organs start filling up after 40% of TUreq have been reached (Eq. (4)). In line with this, the LAI curve reaches a plateau when 45% (wheat) or 50% (other crops) of the TUreq are fulfilled, which could be considered a proxy of the flowering stage.$${{{{{{mathrm{fHI}}}}}}}=100times frac{{{{{{{{mathrm{fTU}}}}}}}}_{{{{{{{mathrm{req}}}}}}}}}{100times {{{{{{{mathrm{fTU}}}}}}}}_{{{{{{{mathrm{req}}}}}}}}+{{exp }}^{11.1-10.0times {{{{{{{mathrm{fTU}}}}}}}}_{{{{{{{mathrm{req}}}}}}}}}}$$ (4) Crop biomass growth is simulated by daily carbon accumulation and allocation to different plant organs (roots, leaves, storage organs, mobile reserves, and stem). The fraction of carbon allocated to each pool is a function of the fraction of completed phenological progress. Water stress increases allocation to the roots and reduces allocation to the leaves. The daily Net Primary Production (NPP) is the result of the Gross Primary Production (daily gross photosynthesis) reduced by the respiration costs. Gross photosynthesis is simulated as a function of absorbed photosynthetically active radiation, CO2 atmospheric mixing ratio, air temperature, day length, and canopy conductance. Photosynthesis rate is given by the minimum between light-limited and Rubisco-limited photosynthesis rates, with distinguished pathways for C3 and C4 crops. Respiration is tissue-specific and it is also driven by temperature. If accumulated NPP is insufficient to satisfy all organ demands, allocation follows a hierarchical order from roots, to leaves, to storage organs, and consequently penalizing the harvest index. Crops are subject to yield failure due to frost events (daily minimum temperature More