Ecology

Ramoliya, P. & Pandey, A. Effect of salinization of soil on emergence, growth and survival of seedlings of Cordia rothii. For. Ecol. Manage. 176, 185–194 (2003).Article 

Google Scholar 
Müller, H. M. et al. The desert plant Phoenix dactylifera closes stomata via nitrate-regulated SLAC1 anion channel. New Phytol. 216, 150–162 (2017).PubMed 
Article 
CAS 

Google Scholar 
Hazzouri, K. M. et al. Prospects for the study and improvement of abiotic stress tolerance in date palms in the post-genomics era. Front. Plant Sci. 11, 293 (2020).PubMed 
PubMed Central 
Article 

Google Scholar 
Abdelfattah, M. A. Integrated suitability assessment: A way forward for land use planning and sustainable development in Abu Dhabi, United Arab Emirates. Arid Land Res. Manage. 27, 41–64 (2013).Article 

Google Scholar 
Al-Muaini, A. et al. Water requirements for irrigation with saline groundwater of three date-palm cultivars with different salt-tolerances in the hyper-arid United Arab Emirates. Agric. Water Manage. 222, 213–220 (2019).Article 

Google Scholar 
Guo, H., Shi, X., Ma, L., Yang, T. & Min, W. Long-term irrigation with saline water decreases soil nutrients, diversity of bacterial communities, and cotton yields in a gray desert soil in China. Pol. J. Environ. Stud. 29, 4077–4088 (2020).CAS 
Article 

Google Scholar 
Blaskó, L. Salinity, physical effects on soils. In Encyclopedia of Agrophysics (eds Gliński, J. et al.) 723–725 (Springer, 2011).Chapter 

Google Scholar 
Rengasamy, P. Irrigation water quality and soil structural stability: A perspective with some new insights. Agronomy 8, 72 (2018).Article 
CAS 

Google Scholar 
Trivedi, P., Leach, J. E., Tringe, S. G., Sa, T. & Singh, B. K. Plant–microbiome interactions: From community assembly to plant health. Nat. Rev. Microbiol. 18, 607–621 (2020).CAS 
PubMed 
Article 

Google Scholar 
Masmoudi, K. et al. Metagenomics of beneficial microbes in abiotic stress tolerance of date palm. In The Date Palm Genome, Vol. 2: Omics and Molecular Breeding (eds Al-Khayri, J. M. et al.) 203–214 (Springer, 2021).Chapter 

Google Scholar 
Boncompagni, E., Østerås, M., Poggi, M.-C. & Le Rudulier, D. Occurrence of choline and glycine betaine uptake and metabolism in the family rhizobiaceae and their roles in osmoprotection. Appl. Environ. Microbiol. 65, 2072–2077 (1999).ADS 
CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Chen, C. & Beattie, G. A. Characterization of the osmoprotectant transporter opuc from Pseudomonas syringae and demonstration that cystathionine-β-synthase domains are required for its osmoregulatory function. J. Bacteriol. 189, 6901–6912 (2007).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Rath, H. et al. Management of osmoprotectant uptake hierarchy in Bacillus subtilis via a SigB-dependent antisense RNA. Front. Microbiol. 11, 622 (2020).PubMed 
PubMed Central 
Article 

Google Scholar 
Singh, R. P. & Jha, P. N. The PGPR Stenotrophomonas maltophilia SBP-9 augments resistance against biotic and abiotic stress in wheat plants. Front. Microbiol. 8, 1945 (2017).PubMed 
PubMed Central 
Article 

Google Scholar 
Ferjani, R. et al. The date palm tree rhizosphere is a niche for plant growth promoting bacteria in the oasis ecosystem. Biomed Res. Int. 2015, 1–10 (2015).Article 

Google Scholar 
Sanka Loganathachetti, D., Alhashmi, F., Chandran, S. & Mundra, S. Irrigation water salinity structures the bacterial communities of date palm (Phoenix dactylifera)-associated bulk soil. Front. Plant Sci. https://doi.org/10.3389/fpls.2022.944637 (2022).Article 

Google Scholar 
Chen, L. J. et al. An integrative influence of saline water irrigation and fertilization on the structure of soil bacterial communities. J. Agric. Sci. 157, 693–700 (2019).CAS 
Article 

Google Scholar 
Li, Y. Q. et al. Bacterial community in saline farmland soil on the Tibetan plateau: Responding to salinization while resisting extreme environments. BMC Microbiol. 21, 119 (2021).PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
Mosqueira, M. J. et al. Consistent bacterial selection by date palm root system across heterogeneous desert oasis agroecosystems. Sci. Rep. 9, 4033 (2019).ADS 
PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
Cherif, H. et al. Oasis desert farming selects environment-specific date palm root endophytic communities and cultivable bacteria that promote resistance to drought. Environ. Microbiol. Rep. 7, 668–678 (2015).CAS 
PubMed 
Article 

Google Scholar 
FAO. Standard Operating Procedure for Soil Electrical Conductivity, Soil/Water, 1:5. (2021).Nelson, D. W. & Sommers, L. E. Total carbon, organic carbon, and organic matter. In Chemical Methods-SSSA Book Series No. 5 (eds Bigham, J. M. et al.) (Soil Science Society of America and American Society of Agronomy, 1996).
Google Scholar 
Mizrahi-Man, O., Davenport, E. R. & Gilad, Y. Taxonomic classification of bacterial 16S rRNA genes using short sequencing reads: Evaluation of effective study designs. PLoS ONE 8, e53608 (2013).ADS 
CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Callahan, B. J. et al. DADA2: High-resolution sample inference from Illumina amplicon data. Nat. Methods 13, 581–583 (2016).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Rognes, T., Flouri, T., Nichols, B., Quince, C. & Mahé, F. VSEARCH: A versatile open source tool for metagenomics. PeerJ 4, e2584 (2016).PubMed 
PubMed Central 
Article 

Google Scholar 
Martin-Sanchez, P. M. et al. Analysing indoor mycobiomes through a large-scale citizen science study in Norway. Mol. Ecol. 30, 2689–2705 (2021).CAS 
PubMed 
Article 

Google Scholar 
Quast, C. et al. The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools. Nucleic Acids Res. 41, D590–D596 (2012).PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
Dai, T. et al. Identifying the key taxonomic categories that characterize microbial community diversity using full-scale classification: A case study of microbial communities in the sediments of Hangzhou Bay. FEMS Microbiol. Ecol. 92, 150 (2016).Article 
CAS 

Google Scholar 
Oksanen, J. et al. vegan: Community Ecology Package (2020).Blanchet, F. G., Legendre, P. & Borcard, D. Forward selection of explanatory variables. Ecology 89, 2623–2632 (2008).PubMed 
Article 

Google Scholar 
Emirates Soil Museum. Emirates Soil Museum. https://www.emiratessoilmuseum.org/index.php/ (Accessed 08 July 2022).Jackson, O., Quilliam, R. S., Stott, A., Grant, H. & Subke, J.-A. Rhizosphere carbon supply accelerates soil organic matter decomposition in the presence of fresh organic substrates. Plant Soil 440, 473–490 (2019).CAS 
Article 

Google Scholar 
Xie, E. et al. Short-term effects of salt stress on the amino acids of Phragmites australis root exudates in constructed wetlands. Water 12, 569 (2020).CAS 
Article 

Google Scholar 
Korber, D. R., Choi, A., Wolfaardt, G. M. & Caldwell, D. E. Bacterial plasmolysis as a physical indicator of viability. Appl. Environ. Microbiol. 62, 3939–3947 (1996).ADS 
CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Zhang, K. et al. Salinity is a key determinant for soil microbial communities in a desert ecosystem. mSystems 4, e00225 (2019).CAS 
PubMed 
PubMed Central 

Google Scholar 
Hessini, K. et al. Interactive effects of salinity and nitrogen forms on plant growth, photosynthesis and osmotic adjustment in maize. Plant Physiol. Biochem. 139, 171–178 (2019).CAS 
PubMed 
Article 

Google Scholar 
Lammel, D. R. et al. Direct and indirect effects of a pH gradient bring insights into the mechanisms driving prokaryotic community structures. Microbiome 6, 106 (2018).PubMed 
PubMed Central 
Article 

Google Scholar 
Lopes, L. D., Hao, J. & Schachtman, D. P. Alkaline soil pH affects bulk soil, rhizosphere and root endosphere microbiomes of plants growing in a Sandhills ecosystem. FEMS Microbiol. Ecol. 97, 028 (2021).Article 
CAS 

Google Scholar 
Rousk, J. et al. Soil bacterial and fungal communities across a pH gradient in an arable soil. ISME J. 4, 1340–1351 (2010).PubMed 
Article 

Google Scholar 
Bahram, M. et al. Structure and function of the global topsoil microbiome. Nature 560, 233–237 (2018).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Kumar, A., Mann, A., Kumar, A., Kumar, N. & Meena, B. L. Physiological response of diverse halophytes to high salinity through ionic accumulation and ROS scavenging. Int. J. Phytoremediat. 23, 1041–1051 (2021).CAS 
Article 

Google Scholar 
Kalam, S. et al. Recent understanding of soil acidobacteria and their ecological significance: A critical review. Front. Microbiol. https://doi.org/10.3389/fmicb.2020.580024 (2020).Article 
PubMed 
PubMed Central 

Google Scholar 
Boukhatem, Z. F., Merabet, C. & Tsaki, H. Plant growth promoting actinobacteria, the most promising candidates as bioinoculants? Front. Agron. https://doi.org/10.3389/fagro.2022.849911 (2022).Article 

Google Scholar 
Köberl, M. et al. Comparisons of diazotrophic communities in native and agricultural desert ecosystems reveal plants as important drivers in diversity. FEMS Microbiol. Ecol. 92, 166 (2016).Article 
CAS 

Google Scholar 
Speirs, L. B. M., Rice, D. T. F., Petrovski, S. & Seviour, R. J. The phylogeny, biodiversity, and ecology of the Chloroflexi in activated sludge. Front. Microbiol. 10, 2015 (2019).PubMed 
PubMed Central 
Article 

Google Scholar 
Hou, Y. et al. Responses of the soil microbial community to salinity stress in maize fields. Biology (Basel) 10, 1114 (2021).CAS 

Google Scholar 
Patil, A., Kale, A., Ajane, G., Sheikh, R. & Patil, S. Plant growth-promoting rhizobium: Mechanisms and biotechnological prospective. Rhizobium Biol. Biotechnol. https://doi.org/10.1007/978-3-319-64982-5_7 (2017).Article 

Google Scholar 
Lima Guimarães, S. et al. Effects of inoculation of Rhizobium on nodulation and nitrogen accumulation in cowpea subjected to water availabilities. Am. J. Plant Sci. 06, 1378–1384 (2015).Article 

Google Scholar 
Ghadbane, M., Medjekal, S., Benderradji, L., Belhadj, H. & Daoud, H. Assessment of arbuscular mycorrhizal fungi status and Rhizobium on date palm (Phoenix dactylifera L.) cultivated in a Pb contaminated soil. In Recent Advances in Environmental Science from the Euro-Mediterranean and Surrounding Regions 2nd edn (eds Ksibi, M. et al.) 703–707 (Springer, 2021).
Google Scholar 
Saeed, E. E. et al. Streptomyces globosus UAE1, a potential effective biocontrol agent for black scorch disease in date palm plantations. Front. Microbiol. 8, 1455 (2017).PubMed 
PubMed Central 
Article 

Google Scholar 
Falagán, C. & Johnson, D. B. Acidibacter ferrireducens gen. nov., sp. nov.: An acidophilic ferric iron-reducing gammaproteobacterium. Extremophiles 18, 1067–1073 (2014).PubMed 
Article 
CAS 

Google Scholar 
Schulze-Makuch, D. et al. Transitory microbial habitat in the hyperarid Atacama desert. Proc. Natl. Acad. Sci. 115, 2670–2675 (2018).ADS 
CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Zhao, K. et al. Actinobacteria associated with Glycyrrhiza inflata Bat. are diverse and have plant growth promoting and antimicrobial activity. Sci. Rep. 8, 13661 (2018).ADS 
PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
An, S.-U. et al. Invasive Spartina anglica greatly alters the rates and pathways of organic carbon oxidation and associated microbial communities in an intertidal wetland of the Han river estuary, Yellow Sea. Front. Mar. Sci. 7, 59 (2020).ADS 
Article 

Google Scholar 
Khan, M. A. et al. Rhizospheric Bacillus spp. rescues plant growth under salinity stress via regulating gene expression, endogenous hormones, and antioxidant system of Oryza sativa L.. Front. Plant Sci. 12, 1145 (2021).
Google Scholar 
Schimel, J., Balser, T. C. & Wallenstein, M. Microbial stress-response physiology and its implications for ecosystem function. Ecology 88, 1386–1394 (2007).PubMed 
Article 

Google Scholar 
Mukhtar, S., Mehnaz, S., Mirza, M. S., Mirza, B. S. & Malik, K. A. Diversity of bacillus-like bacterial community in the rhizospheric and non-rhizospheric soil of halophytes (Salsola stocksii and Atriplex amnicola), and characterization of osmoregulatory genes in halophilic Bacilli. Can. J. Microbiol. 64, 567–579 (2018).CAS 
PubMed 
Article 

Google Scholar 
Yeager, C. M. et al. Polysaccharide degradation capability of actinomycetales soil isolates from a semiarid grassland of the colorado plateau. Appl. Environ. Microbiol. 83, e03020-e3116 (2017).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Ortúzar, M., Trujillo, M. E., Román-Ponce, B. & Carro, L. Micromonospora metallophores: A plant growth promotion trait useful for bacterial-assisted phytoremediation? Sci. Total Environ. 739, 139850 (2020).ADS 
PubMed 
Article 
CAS 

Google Scholar 
El-Tarabily, K. A. et al. Growth promotion of Salicornia bigelovii by Micromonospora chalcea UAE1, an endophytic 1-aminocyclopropane-1-carboxylic acid deaminase-producing actinobacterial isolate. Front. Microbiol. 10, 1694 (2019).PubMed 
PubMed Central 
Article 

Google Scholar 
Carro, L. et al. Genome-based classification of micromonosporae with a focus on their biotechnological and ecological potential. Sci. Rep. 8, 525 (2018).ADS 
PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
Li, M. et al. Composition and function of rhizosphere microbiome of Panax notoginseng with discrepant yields. Chin. Med. 15, 85 (2020).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Rufián, J. S., Rueda-Blanco, J., Beuzón, C. R. & Ruiz-Albert, J. Protocol: An improved method to quantify activation of systemic acquired resistance (SAR). Plant Methods 15, 16 (2019).PubMed 
PubMed Central 
Article 

Google Scholar 
Bhise, K. K., Bhagwat, P. K. & Dandge, P. B. Synergistic effect of Chryseobacterium gleum sp. SUK with ACC deaminase activity in alleviation of salt stress and plant growth promotion in Triticum aestivum L.. 3 Biotech 7, 105 (2017).PubMed 
PubMed Central 
Article 

Google Scholar 
Cao, C., Tao, S., Cui, Z. & Zhang, Y. Response of soil properties and microbial communities to increasing salinization in the meadow grassland of Northeast China. Microb. Ecol. 82, 722–735 (2021).CAS 
PubMed 
Article 

Google Scholar  More

Dauvin, J. C. et al. The well sorted fine sand community from the western Mediterranean Sea: A resistant and resilient marine habitat under diverse human pressures. Environ. Pollut. 224, 336–351 (2017).CAS 
PubMed 
Article 

Google Scholar 
Obolewski, K. & Glińska-Lewczuk, K. Connectivity and complexity of coastal lakes as determinants for their restoration-A case study of the southern Baltic Sea. Ecol. Eng. 155, 1700 (2020).Article 

Google Scholar 
Dobrowolski, Z. Occurrence of macrobenthos in different littoral habitats of the polymictic Lebsko lake. Ekologia Polska 42, 19–40 (1994).
Google Scholar 
Paturej, E., Gutkowska, A. & Durczak, K. Biodiversity and indicative role of zooplankton in the shallow macrophyte-dominated lake Łuknajno. Pol. J. Nat. Sci. 27, 53–66 (2012).
Google Scholar 
Obolewski, K. et al. Patterns of salinity regime in coastal lakes based on structure of benthic invertebrates. PLoS ONE 13, 150 (2018).Article 
CAS 

Google Scholar 
Lew, S., Glińska-Lewczuk, K. & Lew, M. The effects of environmental parameters on the microbial activity in peat-bog lakes. PLoS ONE 14, 179 (2019).
Google Scholar 
Bremner, J. Species’ traits and ecological functioning in marine conservation and management. J. Exp. Mar. Biol. Ecol. 366, 37–47 (2008).Article 

Google Scholar 
Törnroos, A. & Bonsdorff, E. Developing the multitrait concept for functional diversity: Lessons from a system rich in functions but poor in species. Ecol. Appl. 22, 2221–2236 (2012).PubMed 
Article 

Google Scholar 
Baldrighi, E. & Manini, E. Deep-sea meiofauna and macrofauna diversity and functional diversity: are they related?. Mar. Biodivers. 45, 469–488 (2015).Article 

Google Scholar 
Belley, R. & Snelgrove, P. V. R. Relative contributions of biodiversity and environment to benthic ecosystem functioning. Front. Mar. Sci. 3, 7598 (2016).Article 

Google Scholar 
Díaz, S. & Cabido, M. Vive la différence: Plant functional diversity matters to ecosystem processes. Trends Ecol. Evol. 16, 646–655 (2001).Article 

Google Scholar 
Gagic, V. et al. Functional identity and diversity of animals predict ecosystem functioning better than species-based indices. Proc. R. Soc. B Biol. Sci. 282, 689 (2015).
Google Scholar 
Ding, N. et al. Different responses of functional traits and diversity of stream macroinvertebrates to environmental and spatial factors in the Xishuangbanna watershed of the upper Mekong River Basin, China. Sci. Total Environ. 574, 288–299 (2017).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Kenny, A. J. et al. Assessing cumulative human activities, pressures, and impacts on North Sea benthic habitats using a biological traits approach. ICES J. Mar. Sci. 75, 1080–1092 (2018).Article 

Google Scholar 
Llanos, E. N., Saracho Bottero, M. A., Jaubet, M. L., Elías, R. & Garaffo, G. V. Functional diversity in the intertidal macrobenthic community at sewage-affected shores from Southwestern Atlantic. Mar. Pollut. Bull. 157, 7448 (2020).Article 
CAS 

Google Scholar 
Paganelli, D., Marchini, A. & Occhipinti-Ambrogi, A. Functional structure of marine benthic assemblages using Biological Traits Analysis (BTA): A study along the Emilia-Romagna coastline (Italy, North-West Adriatic Sea). Estuar. Coast. Shelf Sci. 96, 245–256 (2012).ADS 
Article 

Google Scholar 
Nasi, F. et al. Functional biodiversity of marine soft-sediment polychaetes from two Mediterranean coastal areas in relation to environmental stress. Mar. Environ. Res. 137, 121–132 (2018).CAS 
PubMed 
Article 

Google Scholar 
Harwell, M. A. et al. Conceptual framework for assessing ecosystem health. Integr. Environ. Assess. Manag. 15, 544–564 (2019).PubMed 
PubMed Central 
Article 

Google Scholar 
Hu, C. et al. Macrobenthos functional trait responses to heavy metal pollution gradients in a temperate lagoon. Environ. Pollut. 253, 1107–1116 (2019).CAS 
PubMed 
Article 

Google Scholar 
Ramsay, K., Kaiser, M. J. & Hughes, R. N. Responses of benthic scavengers to fishing disturbance by towed gears in different habitats. J. Exp. Mar. Biol. Ecol. 224, 4458 (1998).Article 

Google Scholar 
Sigala, K., Reizopoulou, S., Basset, A. & Nicolaidou, A. Functional diversity in three Mediterranean transitional water ecosystems. Estuar. Coast. Shelf Sci. 110, 202–209 (2012).ADS 
Article 

Google Scholar 
de Loiola, P. P., Cianciaruso, M. V., Silva, I. A. & Batalha, M. A. Functional diversity of herbaceous species under different fire frequencies in Brazilian savannas. Flora Morphol. Distrib. Funct. Ecol. Plants 205, 674–681 (2010).Article 

Google Scholar 
Schleuter, D., Daufresne, M., Massol, F. & Argillier, C. A user’s guide to functional diversity indices. Ecological Monographs vol. 80 http://www.scopus.com/scopus/search/form.urli (2010).Wan, H. W. M. R., Cooper, K. M., Froján, C. R. S. B., Defew, E. C. & Paterson, D. M. Impacts of physical disturbance on the recovery of a macrofaunal community: A comparative analysis using traditional and novel approaches. Ecol. Indicators 12, 37–45 (2012).Article 

Google Scholar 
Millet, B. & Guelorget, O. Spatial and seasonal variability in the relationships between benthic communities and physical environment in a lagoon ecosystem. Mar. Ecol. Prog. Ser. 108, 161–174 (1994).ADS 
Article 

Google Scholar 
McLusky, D. S. & Elliott, M. The Estuarine Ecosystem (Oxford University Press, 2004). https://doi.org/10.1093/acprof:oso/9780198525080.001.0001.Book 

Google Scholar 
Mrozińska, N. & Bąkowska, M. Effects of heavy metals in lake water and sediments on bottom invertebrates inhabiting the brackish coastal lake Łebsko on the southern baltic coast. Int. J. Environ. Res. Public Health 17, 1–19 (2020).Article 
CAS 

Google Scholar 
Petchey, O. L. & Gaston, K. J. Functional diversity: Back to basics and looking forward. Ecol. Lett. 9, 741–758 (2006).PubMed 
Article 

Google Scholar 
Villéger, S., Miranda, J. R., Hernández, D. F. & Mouillot, D. Contrasting changes in taxonomic vs. functional diversity of tropical fish communities after habitat degradation. Ecol. Appl. 20, 1512–1522 (2010).PubMed 
Article 

Google Scholar 
Dolédec, S. & Statzner, B. Theoretical habitat templets, species traits, and species richness: 548 plant and animal species in the Upper Rhône River and its floodplain. Freshw. Biol. 31, 523–538 (1994).Article 

Google Scholar 
Usseglio-Polatera, P., Bournaud, M., Richoux, P. & Tachet, H. Biomonitoring through biological traits of benthic macroinvertebrates: How to use species trait databases?. Hydrobiologia 422, 153–162 (2000).Article 

Google Scholar 
Charvet, S., Statzner, B., Usseglio-Polatera, P. & Dumont, B. Traits of benthic macroinvertebrates in semi-natural French streams: An initial application to biomonitoring in Europe. Freshw. Biol. 43, 277–296 (2000).Article 

Google Scholar 
Statzner, B., Dolédec, S. & Hugueny, B. Biological trait composition of European stream invertebrate communities: Assessing the effects of various trait filter types. Ecography 27, 470–488 (2004).Article 

Google Scholar 
Bremner, J., Rogers, S. I. & Frid, C. L. J. Assessing functional diversity in marine benthic ecosystems: A comparison of approaches. Mar Ecol Prog Ser 254, 5589 (2003).Article 

Google Scholar 
Tillin, H., Hiddink, J., Jennings, S. & Kaiser, M. Chronic bottom trawling alters the functional composition of benthic invertebrate communities on a sea-basin scale. Mar. Ecol. Prog. Ser. 318, 31–45 (2006).ADS 
Article 

Google Scholar 
Marchini, A., Munari, C. & Mistri, M. Functions and ecological status of eight Italian lagoons examined using biological traits analysis (BTA). Mar. Pollut. Bull. 56, 1076–1085 (2008).CAS 
PubMed 
Article 

Google Scholar 
Boikova, E., Botva, U. & Līcīte, V. Implementation of trophic status index in brackish water quality assessment of baltic coastal waters. Proc. Latv. Acad. Sci. Sect. B 62, 115–119 (2008).CAS 

Google Scholar 
Wielgat-Rychert, M., Jarosiewicz, A., Ficek, D., Pawlik, M. & Rychert, K. Nutrient fluxes and their impact on the phytoplankton in a Shallow Coastal Lake. Polish J. Environ. Stud. 24, 7780 (2015).Article 
CAS 

Google Scholar 
Kruk, C., Devercelli, M. & Huszar, V. L. Reynolds Functional Groups: A trait-based pathway from patterns to predictions. Hydrobiologia 848, 113–129 (2021).Article 

Google Scholar 
Trojanowski, J., Trojanowska, C. & Korzeniewski, K. Trophic state of coastal lakes. Polish Arch. Hydrobiol. 38, 23–34 (1975).
Google Scholar 
Astel, A. M., Bigus, K., Obolewski, K. & Glińska-Lewczuk, K. Spatiotemporal assessment of water chemistry in intermittently open/closed coastal lakes of Southern Baltic. Estuar. Coast. Shelf Sci. 182, 47–59 (2016).ADS 
CAS 
Article 

Google Scholar 
Choiński, A. Changes in morphometrics of the coastal lakes. in Hydroecological Determinants of Functioning of Southern Baltic Coastal Lakes (eds. Obolewski, K., Astel, A. & Kujawa, R.) 26–37 (PWN, 2017).Obolewski, K., Glińska-Lewczuk, K., Bąkowska, M., Szymańska, M. & Mrozińska, N. Patterns of phytoplankton composition in coastal lakes differed by connectivity with the Baltic Sea. Sci. Total Environ. 631–632, 951–961 (2018).ADS 
PubMed 
Article 
CAS 

Google Scholar 
Szymańska-Walkiewicz, M., Glińska-Lewczuk, K., Burandt, P. & Obolewski, K. Phytoplankton sensitivity to heavy metals in Baltic Coastal Lakes. Int. J. Environ. Res. Public Health 19, 4131 (2022).PubMed 
PubMed Central 
Article 
CAS 

Google Scholar 
Mrozińska, N., Glińska-Lewczuk, K. & Obolewski, K. Salinity as a key factor on the benthic fauna diversity in the coastal lakes. Animals 11, 7440 (2021).Article 

Google Scholar 
Bremner, J., Rogers, S. I. & Frid, C. L. J. Methods for describing ecological functioning of marine benthic assemblages using biological traits analysis (BTA). Ecol. Ind. 6, 609–622 (2006).Article 

Google Scholar 
Papageorgiou, N., Sigala, K. & Karakassis, I. Changes of macrofaunal functional composition at sedimentary habitats in the vicinity of fish farms. Estuar. Coast. Shelf Sci. 83, 561–568 (2009).ADS 
CAS 
Article 

Google Scholar 
Lam-Gordillo, O., Baring, R. & Dittmann, S. Ecosystem functioning and functional approaches on marine macrobenthic fauna: A research synthesis towards a global consensus. Ecol Indic 115, 5589 (2020).Article 

Google Scholar 
Kołodziejczyk, A. & Koperski, P. Bezkręgowce słodkowodne Polski: klucz do oznaczania oraz podstawy biologii i ekologii makrofauny. (Wydawnictwa Uniwersytetu Warszawskiego, 2000).Wiederholm, Torgny. Chironomidae of the Holarctic Region: Keys and Diagnoses. Part 1: larvae. (1983).Antsulevich, A. et al. Helcom, 2012. Development of a set of core indicators: Interim report of the HELCOM CORESET project. PART A. Description of the selection process. (2012).Piechocki, A. & Wawrzyniak-Wydrowska, B. Guide to Freshwater and Marine Mollusca of Poland. (2016).Zettler, M. L. et al. Biodiversity gradient in the Baltic Sea: A comprehensive inventory of macrozoobenthos data. Helgol. Mar. Res. 68, 49–57 (2014).ADS 
Article 

Google Scholar 
Palomares, M. L. D. & Pauly, D. SeaLifeBase. https://www.sealifebase.ca/ (2021).MarLIN. BIOTIC-biological traits information catalogue. Marine Life Information Network. Plymouth: Marine Biological Association of the UK. http://www.marlin.ac.uk/biotic/ (2006).Horton, T. et al. World Register of Marine Species (WoRMS). https://www.marinespecies.org (2021).Chevene, F., Doleadec, S. & Chessel, D. A fuzzy coding approach for the analysis of long-term ecological data. Freshw. Biol. 31, 295–309 (1994).Article 

Google Scholar 
Oug, E., Fleddum, A., Rygg, B. & Olsgard, F. Biological traits analyses in the study of pollution gradients and ecological functioning of marine soft bottom species assemblages in a fjord ecosystem. J. Exp. Mar. Biol. Ecol. 432–433, 94–105 (2012).Article 

Google Scholar 
Egres, A. G., Hatje, V., Miranda, D. A., Gallucci, F. & Barros, F. Functional response of tropical estuarine benthic assemblages to perturbation by Polycyclic Aromatic Hydrocarbons. Ecol. Ind. 96, 229–240 (2019).CAS 
Article 

Google Scholar 
Charvet, S., Kosmala, A. & Statzner, B. Biomonitoring through biological traits of benthic macroinvertebrates: Perspectives for a general tool in stream management. Fundam. Appl. Limnol. 142, 415–432 (1998).Article 

Google Scholar 
Clarke, K. R. & Gorley, R. N. PRIMER v6: User Manual/Tutorial. (2006).Dobrowolski, Z. Density, biomass, and distribution of benthic invertebrates in the mid-lake zone of the coastal Lake Gardno. Oceanol. Stud. 30, 39–58 (2001).
Google Scholar 
Michaud, E., Desrosiers, G., Mermillod-Blondin, F., Sundby, B. & Stora, G. The functional group approach to bioturbation: II. The effects of the Macoma balthica community on fluxes of nutrients and dissolved organic carbon across the sediment-water interface. J. Exp. Mar. Biol. Ecol. 337, 178–189 (2006).CAS 
Article 

Google Scholar 
Taurusman, A. A. Community structure of macrozoobenthic feeding guilds in responses to eutrophication in Jakarta Bay. Biodivers. J. Biol. Divers. 11, 998 (2010).Article 

Google Scholar 
Uwadiae, R. E. Macroinvertebrates functional feeding groups as indices of biological assessment in a tropical aquatic ecosystem: implications for ecosystem functions. New York Sci. J. 3, 778 (2010).
Google Scholar 
Obolewski, K., Glińska-Lewczuk, K., Sidoruk, M. & Szymańska, M. M. Response of benthic fauna to habitat heterogeneity in a shallow temperate lake. Animals 11, 558 (2021).Article 

Google Scholar 
Rhoads, D. C. Organism-sediment relations on the muddy sea floor. in Oceanography and Marine Biology: An Annual Review. vol. 12 263–300 (Aberdeen University Press/Allen & Unwin, 1974).Thrush, S. F., Hewitt, J. E., Gibbs, M., Lundquist, C. & Norkko, A. Functional role of large organisms in intertidal communities: Community effects and ecosystem function. Ecosystems 9, 1029–1040 (2006).Article 

Google Scholar 
Frid, C. L. J., Harwood, K. G., Hall, S. J. & Hall, J. A. Long-term changes in the benthic communities on North Sea fishing grounds. in ICES Journal of Marine Science vol. 57 1303–1309 (Academic Press, 2000).Bradshaw, C., Veale, L. O. & Brand, A. R. The role of scallop-dredge disturbance in long-term changes in Irish Sea benthic communities: A re-analysis of an historical dataset. J. Sea Res. 47, 161–184 (2002).ADS 
Article 

Google Scholar 
Cañedo-Argüelles, M. et al. Can salinity trigger cascade effects on streams? A mesocosm approach. Sci. Total Environ. 540, 3–10 (2016).ADS 
PubMed 
Article 
CAS 

Google Scholar 
Herbst, D. B. Salinity controls on trophic interactions among invertebrates and algae of solar evaporation ponds in the Mojave Desert and relation to shorebird foraging and selenium risk. Wetlands 26, 475–485 (2006).Article 

Google Scholar 
Merritt, R. W. et al. Development and application of a macroinvertebrate functional-group approach in the bioassessment of remnant river oxbows in southwest Florida. Am. Benthol. Soc. 21, 550 (2002).Article 

Google Scholar 
de Roos, A. M., Persson, L. & McCauley, E. The influence of size-dependent life-history traits on the structure and dynamics of populations and communities. Ecol. Lett. 6, 473–487 (2003).Article 

Google Scholar 
Reizopoulou, S. & Nicolaidou, A. Index of size distribution (ISD): A method of quality assessment for coastal lagoons. Hydrobiologia 577, 141–149 (2007).Article 

Google Scholar 
Basset, A., Pinna, M., Sabetta, L., Barbone, E. & Galuppo, N. Hierarchical scaling of biodiversity in lagoon ecosystems. Trans. Waters Bull. 2, 75–86 (2008).
Google Scholar 
Basset, A. et al. A benthic macroinvertebrate size spectra index for implementing the Water Framework Directive in coastal lagoons in Mediterranean and Black Sea ecoregions. Ecol. Ind. 12, 72–83 (2012).Article 

Google Scholar 
Robson, B. J., Barmuta, L. A. & Fairweather, P. G. Methodological and conceptual issues in the search for a relationship between animal body-size distributions and benthic habitat architecture. Mar. Freshw. Res. 56, 1–11 (2005).Article 

Google Scholar 
Parry, D. M., Kendall, M. A., Rowden, A. A. & Widdicombe, S. Species body size distribution patterns of marine benthic macrofauna assemblages from contrasting sediment types. J. Mar. Biol. Assoc. U.K. 79, 793–801 (1999).Article 

Google Scholar 
Netto, S. A., Domingos, A. M. & Kurtz, M. N. Effects of artificial breaching of a temporarily open/closed estuary on benthic macroinvertebrates (Camacho Lagoon, Southern Brazil). Estuaries Coasts 35, 1069–1081 (2012).CAS 
Article 

Google Scholar 
Folke, C. et al. Regime shifts, resilience, and biodiversity in ecosystem management. Annu. Rev. Ecol. Evol. Syst. 35, 557–581 (2004).Article 

Google Scholar 
Montefalcone, M., Parravicini, V. & Bianchi, C. N. Quantification of Coastal Ecosystem Resilience. in Treatise on Estuarine and Coastal Science 49–70 (Elsevier, 2011). https://doi.org/10.1016/B978-0-12-374711-2.01003-2.Sasaki, T., Furukawa, T., Iwasaki, Y., Seto, M. & Mori, A. S. Perspectives for ecosystem management based on ecosystem resilience and ecological thresholds against multiple and stochastic disturbances. Ecol. Ind. 57, 395–408 (2015).Article 

Google Scholar 
Smee, D. L., Reustle, J. W., Belgrad, B. A. & Pettis, E. L. Storms promote ecosystem resilience by alleviating fishing. Curr. Biol. 30, R869–R870 (2020).CAS 
PubMed 
Article 

Google Scholar 
Gilby, B. L. et al. Umbrellas can work under water: Using threatened species as indicator and management surrogates can improve coastal conservation. Estuar. Coast. Shelf Sci. 199, 132–140 (2017).ADS 
Article 

Google Scholar 
Henderson, C. J. et al. Landscape transformation alters functional diversity in coastal seascapes. Ecography 43, 138–148 (2020).Article 

Google Scholar 
Yeager, L. A., Geyer, J. K. & Fodrie, F. J. Trait sensitivities to seagrass fragmentation across spatial scales shape benthic community structure. J. Anim. Ecol. 88, 1743–1754 (2019).PubMed 
Article 

Google Scholar 
Darr, A., Gogina, M. & Zettler, M. L. Functional changes in benthic communities along a salinity gradient- a western Baltic case study. J. Sea Res. 85, 315–324 (2014).ADS 
Article 

Google Scholar 
Statzner, B., Bady, P., Dolédec, S. & Schöll, F. Invertebrate traits for the biomonitoring of large European rivers: An initial assessment of trait patterns in least impacted river reaches. Freshw. Biol. 50, 2136–2161 (2005).Article 

Google Scholar  More

Hanski, I. Habitat fragmentation and species richness. J. Biogeogr. 42, 989–993. https://doi.org/10.1111/jbi.12478 (2015).Article 

Google Scholar 
Hoffmann, M. et al. The impact of conservation on the status of the world’s vertebrates. Science 330, 1503–1509. https://doi.org/10.1126/science.1194442 (2010).ADS 
CAS 
Article 
PubMed 

Google Scholar 
Pimm, S. L. et al. The biodiversity of species and their rates of extinction, distribution, and protection. Science 344, 1246752 (2014).CAS 
Article 

Google Scholar 
Crooks, K. R. Relative sensitivities of mammalian carnivores to habitat fragmentation. Conserv. Biol. 16, 488–502. https://doi.org/10.1046/j.1523-1739.2002.00386.x (2002).Article 

Google Scholar 
Elliot, N. B., Cushman, S. A., Macdonald, D. W. & Loveridge, A. J. The devil is in the dispersers: Predictions of landscape connectivity change with demography. J. Appl. Ecol. 51, 1169–1178. https://doi.org/10.1111/1365-2664.12282 (2014).Article 

Google Scholar 
Carroll, C. Interacting effects of climate change, landscape conversion, and harvest on carnivore populations at the range margin: Marten and lynx in the northern Appalachians. Conserv. Biol. 21, 1092–1104. https://doi.org/10.1111/j.1523-1739.2007.00719.x (2007).Article 
PubMed 

Google Scholar 
Ripple, W. J. et al. Status and ecological effects of the world’s largest carnivores. Science 343, 1241484. https://doi.org/10.1126/science.1241484 (2014).CAS 
Article 
PubMed 

Google Scholar 
Farris, Z. J. et al. Hunting, exotic carnivores, and habitat loss: Anthropogenic effects on a native carnivore community, Madagascar. PLOS ONE 10, e0136456. https://doi.org/10.1371/journal.pone.0136456 (2015).CAS 
Article 
PubMed 
PubMed Central 

Google Scholar 
Farris, Z. J. et al. Threats to a rainforest carnivore community: A multi-year assessment of occupancy and co-occurrence in Madagascar. Biol. Cons. 210, 116–124. https://doi.org/10.1016/j.biocon.2017.04.010 (2017).Article 

Google Scholar 
Swihart, R. K., Gehring, T. M., Kolozsvary, M. B. & Nupp, T. E. Responses of “resistant” vertebrates to habitat loss and fragmentation: The importance of niche breadth and range boundaries. Divers. Distrib. 9, 1–18. https://doi.org/10.1046/j.1472-4642.2003.00158.x (2003).Article 

Google Scholar 
Caryl, F. M., Quine, C. P. & Park, K. J. Martens in the matrix: The importance of nonforested habitats for forest carnivores in fragmented landscapes. J. Mammal. 93, 464–474. https://doi.org/10.1644/11-MAMM-A-149.1 (2012).Article 

Google Scholar 
Pereboom, V. et al. Movement patterns, habitat selection, and corridor use of a typical woodland-dweller species, the European pine marten (Martes martes), in fragmented landscape. Can. J. Zool. 86, 983–991. https://doi.org/10.1139/Z08-076 (2008).Article 

Google Scholar 
Fleschutz, M. M. et al. Response of a small felid of conservation concern to habitat fragmentation. Biodivers. Conserv. 25, 1447–1463. https://doi.org/10.1007/s10531-016-1118-6 (2016).Article 

Google Scholar 
Gálvez, N. et al. Forest cover outside protected areas plays an important role in the conservation of the Vulnerable guiña Leopardus guigna. Oryx 47, 251–258. https://doi.org/10.1017/S0030605312000099 (2013).Article 

Google Scholar 
Belcher, C. A. Demographics of tiger quoll (Dasyurus maculatus maculatus) populations in south-eastern Australia. Aust. J. Zool. 51, 611–626. https://doi.org/10.1071/ZO02051 (2003).Article 

Google Scholar 
Maxwell, S., Burbidge, A. & Morris, K. Spotted-tailed Quoll (SE mainland and Tas); recovery outline. (1996).Jones, M. E., Rose, R. K. & Burnett, S. Dasyurus maculatus. Mammalian Species 676, 1–9 (2001).Article 

Google Scholar 
Long, K. & Nelson, J. National recovery plan for the spotted-tailed Quoll Dasyurus maculatus. Victorian Department of Sustainability and Environment (2010).Claridge, A. W. et al. Home range of the spotted-tailed quoll (Dasyurus maculatus), a marsupial carnivore, in a rainshadow woodland. Wildl. Res. 32, 7–14. https://doi.org/10.1071/WR04031 (2005).Article 

Google Scholar 
Glen, A. S. & Dickman, C. R. Home range, denning behaviour and microhabitat use of the carnivorous marsupial Dasyurus maculatus in eastern Australia. J. Zool. 268, 347–354. https://doi.org/10.1111/j.1469-7998.2006.00064.x (2006).Article 

Google Scholar 
Körtner, G. et al. Population structure, turnover and movement of spotted-tailed quolls on the New England Tablelands. Wildl. Res. 31, 475–484. https://doi.org/10.1071/WR03041 (2004).Article 

Google Scholar 
Belcher, C. The Largest Surviving Marsupial Carnivore on Mainland Australia: The Tiger or Spotted-Tailed Quoll Dasyurus maculatus, A Nationally Threatened, Forest-Dependent Species 612–623 (Royal Zoological Society of New South Wales, Sydney, 2004).
Google Scholar 
Henderson, T., Fancourt, B. A., Rajaratnam, R., Vernes, K. & Ballard, G. Spatial and temporal interactions between endangered spotted-tailed quolls and introduced red foxes in a fragmented landscape. J. Zool. https://doi.org/10.1111/jzo.12919 (2021).Article 

Google Scholar 
Troy, S. N. Spatial Ecology of the Tasmanian Spotted-Tailed Quoll. Ph.D. Thesis, University of Tasmania, (2014).Jones, M. E. et al. Research supporting restoration aiming to make a fragmented landscape ‘functional’ for native wildlife. Ecol. Manag. Restor. 22, 65–74. https://doi.org/10.1111/emr.12504 (2021).Article 

Google Scholar 
Andersen, G. E., Johnson, C. N., Barmuta, L. A. & Jones, M. E. Use of anthropogenic linear features by two medium-sized carnivores in reserved and agricultural landscapes. Scientific Reports 7, 1–11. https://doi.org/10.1038/s41598-017-11454-z (2017).CAS 
Article 

Google Scholar 
Nichols, J. D. in Applied Ecology and Human Dimensions in Biological Conservation (eds L. M. Verdade, M.C. Lyra-Jorge, & C.I. Pina) 117–131 (Springer, 2014).Royle, J. A., Chandler, R. B., Sollmann, R. & Gardner, B. in Spatial Capture-Recapture (eds J. Andrew Royle, Richard B. Chandler, Rahel Sollmann, & Beth Gardner) 3–19 (Academic Press, 2014).Sollmann, R., Gardner, B. & Belant, J. L. How does spatial study design influence density estimates from spatial capture-recapture models?. PLoS ONE 7, e34575 (2012).ADS 
CAS 
Article 

Google Scholar 
Kalle, R., Ramesh, T., Qureshi, Q. & Sankar, K. Density of tiger and leopard in a tropical deciduous forest of Mudumalai Tiger Reserve, southern India, as estimated using photographic capture–recapture sampling. Acta Theriol. 56, 335–342. https://doi.org/10.1007/s13364-011-0038-9 (2011).Article 

Google Scholar 
Vissia, S., Wadhwa, R. & van Langevelde, F. Co-occurrence of high densities of brown hyena and spotted hyena in central Tuli, Botswana. J. Zool. 314, 143–150. https://doi.org/10.1111/jzo.12873 (2021).Article 

Google Scholar 
Henderson, T., Fancourt, B. A. & Ballard, G. The importance of species-specific survey designs: Prey camera trap surveys significantly underestimate the detectability of endangered spotted-tailed quolls. Aust. Mammalogy https://doi.org/10.1071/AM21039 (2022).Gorta, S. B. Z., Alting, B., Claridge, A. & Henderson, T. Apparent piebald variants in quolls (Dasyurus): Examples of three recent cases in the spotted-tailed quoll Dasyurus maculatus. Aust. Mammalogy 43, 373–377. https://doi.org/10.1071/AM20058 (2021).Article 

Google Scholar 
Kowalksi, M. (https://exifpro.informer.com/2.1/, 2011).Efford, M. in R package version 4.5.3 (2022).R Core Team. (R Foundation for Statistical Computing, Vienna, Austria, 2022).Rovero, F. & Zimmermann, F. Camera Trapping for Wildlife Research (Pelagic Publishing Ltd, London, 2016).
Google Scholar 
Efford, M. Density estimation in live-trapping studies. Oikos 106, 598–610. https://doi.org/10.1111/j.0030-1299.2004.13043.x (2004).Article 

Google Scholar 
Niedballa, J., Sollmann, R., Courtiol, A. & Wilting, A. camtrapR: An R package for efficient camera trap data management. Methods Ecol. Evol. 7, 1457–1462. https://doi.org/10.1111/2041-210X.12600 (2016).Article 

Google Scholar 
Burnham, K. P. & Anderson, D. R. A practical information-theoretic approach. Model Sel. Multimodel Inference 2, 70–71 (2002).MATH 

Google Scholar 
Hamer, R. P. et al. Differing effects of productivity on home-range size and population density of a native and an invasive mammalian carnivore. Wildlife Res. 49, 158–168. https://doi.org/10.1071/WR20134 (2021).Article 

Google Scholar 
Glen, A. S. & Dickman, C. R. Complex interactions among mammalian carnivores in Australia, and their implications for wildlife management. Biol. Rev. 80, 387–401. https://doi.org/10.1017/s1464793105006718 (2005).Article 
PubMed 

Google Scholar 
Glen, A. S., Pennay, M., Dickman, C. R., Wintle, B. A. & Firestone, K. B. Diets of sympatric native and introduced carnivores in the Barrington Tops, eastern Australia. Austral Ecol. 36, 290–296. https://doi.org/10.1111/j.1442-9993.2010.02149.x (2011).Article 

Google Scholar 
Glen, A. S. & Dickman, C. R. Population viability analysis shows spotted-tailed quolls may be vulnerable to competition. Aust Mammalogy 35, 180–183. https://doi.org/10.1071/AM12045 (2013).Article 

Google Scholar 
Graham, C. A., Maron, M. & McAlpine, C. A. Influence of landscape structure on invasive predators: Feral cats and red foxes in the brigalow landscapes, Queensland Australia. Wildl. Res. 39, 661–676. https://doi.org/10.1071/WR12008 (2012).Article 

Google Scholar 
Glen, A. S. Population attributes of the spotted-tailed quoll (Dasyurus maculatus) in north-eastern New South Wales. Aust. J. Zool. 56, 137–142. https://doi.org/10.1071/ZO08025 (2008).Article 

Google Scholar 
Chua, M. A., Sivasothi, N. & Meier, R. Population density, spatiotemporal use and diet of the leopard cat (Prionailurus bengalensis) in a human-modified succession forest landscape of Singapore. Mammal Res. 61, 99–108 (2016).Article 

Google Scholar 
Lorica, M. & Heaney, L. Survival of a native mammalian carnivore, the leopard cat Prionailurus bengalensis Kerr, 1792 (Carnivora: Felidae), in an agricultural landscape on an oceanic Philippine island. J. Threatened Taxa, 4451–4460 (2013).Rajaratnam, R., Sunquist, M., Rajaratnam, L. & Ambu, L. Diet and habitat selection of the leopard cat (Prionailurus bengalensis borneoensis) in an agricultural landscape in Sabah, Malaysian Borneo. J. Trop. Ecol. 23, 209–217 (2007).Article 

Google Scholar 
Belcher, C. A. & Darrant, J. P. Den use by the spotted-tailed quoll Dasyurus maculatus in south-eastern Australia. Aust Mammalogy 28, 59–64. https://doi.org/10.1071/AM06007 (2006).Article 

Google Scholar 
Glen, A. & Dickman, C. Why are there so many spotted-tailed Quolls Dasyurus maculatus in parts of north-eastern New South Wales?. Aust Zool 35, 711–718. https://doi.org/10.7882/az.2011.023 (2011).Article 

Google Scholar 
Hanski, I. Metapopulation ecology (Oxford University Press, Oxford, 1999).
Google Scholar 
Pulliam, H. R. Sources, sinks, and population regulation. Am. Nat. 132, 652–661 (1988).Article 

Google Scholar 
Belcher, C. A. Susceptibility of the tiger quoll, Dasyurus maculatus, and the eastern quoll, D. viverrinus, to 1080-poisoned baits in control programmes for vertebrate pests in eastern Australia. Wildl. Res. 25, 33–40. https://doi.org/10.1071/WR95077 (1998).Article 

Google Scholar 
Schmidt, G. M., Graves, T. A., Pederson, J. C. & Carroll, S. L. Precision and bias of spatial capture–recapture estimates: A multi-site, multi-year Utah black bear case study. Ecological Applications 32, e2618. https://doi.org/10.1002/eap.2618 (2022).Article 
PubMed 
PubMed Central 

Google Scholar 
White, G. C. Capture-Recapture and Removal Methods for Sampling Closed Populations (Los Alamos National Laboratory, New Mexico, 1982).
Google Scholar 
Thornton, D. H. & Pekins, C. E. Spatially explicit capture–recapture analysis of bobcat (Lynx rufus) density: Implications for mesocarnivore monitoring. Wildl. Res. 42, 394–404. https://doi.org/10.1071/WR15092 (2015).Article 

Google Scholar 
Sollmann, R. et al. Improving density estimates for elusive carnivores: Accounting for sex-specific detection and movements using spatial capture–recapture models for jaguars in central Brazil. Biol. Cons. 144, 1017–1024 (2011).Article 

Google Scholar 
Green, A. M., Chynoweth, M. W. & Şekercioğlu, Ç. H. Spatially explicit capture-recapture through camera trapping: A review of benchmark analyses for wildlife density estimation. Front. Ecol. Evol. 8, 473. https://doi.org/10.3389/fevo.2020.563477 (2020).Article 

Google Scholar 
du Preez, B. D., Loveridge, A. J. & Macdonald, D. W. To bait or not to bait: a comparison of camera-trapping methods for estimating leopard Panthera pardus density. Biol. Cons. 176, 153–161 (2014).Article 

Google Scholar 
Zimmermann, F., Breitenmoser-Würsten, C., Molinari-Jobin, A. & Breitenmoser, U. Optimizing the size of the area surveyed for monitoring a Eurasian lynx (Lynx lynx) population in the Swiss Alps by means of photographic capture–recapture. Integr. Zool. 8, 232–243 (2013).Article 

Google Scholar 
Dupont, P., Milleret, C., Gimenez, O. & Bischof, R. Population closure and the bias-precision trade-off in spatial capture–recapture. Methods Ecol. Evol. 10, 661–672. https://doi.org/10.1111/2041-210X.13158 (2019).Article 

Google Scholar 
Mergey, M., Helder, R. & Roeder, J. J. Effect of forest fragmentation on space-use patterns in the European pine marten (Martes martes). J. Mammal. 92, 328–335. https://doi.org/10.1644/09-MAMM-A-366.1 (2011).Article 

Google Scholar 
Silmi, M. et al. Activity and ranging behavior of leopard cats (Prionailurus bengalensis) in an oil palm landscape. Frontiers in Environmental Science 9, 651939. https://doi.org/10.3389/fenvs.2021.651939 (2021).Article 

Google Scholar  More

Ravishankara, A. R., Daniel, J. S. & Portmann, R. W. Nitrous Oxide (N2O): The Dominant Ozone-Depleting Substance Emitted in the 21st Century. Science 326, 123–125 (2009).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Tian H. et al. A comprehensive quantification of global nitrous oxide sources and sinks. Nature. 586, 248–256 (2020).WMO. WMO Greenhouse Gas Bulletin: The State of Greenhouse Gases in the Atmosphere Based on Global Observations through 2019. Tech. Rep. (2020).Butterbach-Bahl, K., Stange, F., Papen, H. & Li, C. Regional inventory of nitric oxide and nitrous oxide emissions for forest soils of southeast Germany using the biogeochemical model PnET-N-DNDC. J. Geophys. Res. – Atmospheres 106, 34155–34166 (2001).ADS 
CAS 
Article 

Google Scholar 
Kesik, M. et al. Inventories of N2O and NO emissions from European forest soils. Biogeosciences 2, 353–375 (2005).ADS 
CAS 
Article 

Google Scholar 
Park, S. et al. Trends and seasonal cycles in the isotopic composition of nitrous oxide since 1940. Nat. Geosci. 5, 261–265 (2012).ADS 
CAS 
Article 

Google Scholar 
World Bank. World Development Indicators: Fertiliser consumption (AG.CON.FERT.ZS), https://data.worldbank.org/indicator/AG.CON.FERT.ZS (2019).Tian, H. et al. Global soil nitrous oxide emissions since the preindustrial era estimated by an ensemble of terrestrial biosphere models: Magnitude, attribution, and uncertainty. Glob. Change Biol. 25, 640–659 (2019).ADS 
Article 

Google Scholar 
Hurtt, G. et al. Harmonization of global land-use change and management for the period 850-2100 (LUH2) for CMIP6. Geosci. Model Dev. 13, 5425–5464 (2020).Sebilo, M., Mayer, B., Nicolardot, B., Pinay, G. & Mariotti, A. Long-term fate of nitrate fertilizer in agricultural soils. Proc. Natl Acad. Sci. USA 110, 18185–18189 (2013).ADS 
CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Galloway, J. N. et al. Transformation of the Nitrogen Cycle: Recent Trends, Questions, and Potential Solutions. Science 320, 889–892 (2008).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Fowler, D. et al. The global nitrogen cycle in the twenty-first century. Philos. Trans. R. Soc. Lond. Ser. B, Biol. Sci. 368, 1–13 (2013).
Google Scholar 
Roy, E. D., Hammond Wagner, C. R. & Niles, M. T. Hot spots of opportunity for improved cropland nitrogen management across the United States. Environ. Res. Lett. 16, (2021).Lett, S. & Michelsen, A. Seasonal variation in nitrogen fixation and effects of climate change in a subarctic heath. Plant Soil 379, 193–204 (2014).CAS 
Article 

Google Scholar 
Wang, W. et al. Characteristics of Atmospheric Reactive Nitrogen Deposition in Nyingchi City. Sci. Rep. 9, 1–11 (2019).ADS 
Article 
CAS 

Google Scholar 
Verma, P. & Sagar, R. Effect of nitrogen (N) deposition on soil-N processes: a holistic approach. Sci. Rep. 10, 1–16 (2020).Article 
CAS 

Google Scholar 
Peng, J. et al. Global Carbon Sequestration Is Highly Sensitive to Model-Based Formulations of Nitrogen Fixation. Glob. Biogeochem. Cycles 34, 1–15 (2020).Article 
CAS 

Google Scholar 
Leitner, S. et al. Closing maize yield gaps in sub-Saharan Africa will boost soil N2O emissions. Curr. Opin. Environ. Sustain. 47, 95–105 (2020).Article 

Google Scholar 
Venterea, R. T. et al. Challenges and opportunities for mitigating nitrous oxide emissions from fertilized cropping systems. Front. Ecol. Environ. 10, 562–570 (2012).Article 

Google Scholar 
Wagner-Riddle, C., Baggs, E. M., Clough, T. J., Fuchs, K. & Petersen, S. O. Mitigation of nitrous oxide emissions in the context of nitrogen loss reduction from agroecosystems: managing hot spots and hot moments. Curr. Opin. Environ. Sustain. 47, 46–53 (2020).Article 

Google Scholar 
Gruber, N. & Galloway, J. N. An Earth-system perspective of the global nitrogen cycle. Nature 451, 293–296 (2008).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Hartmann, A. A., Barnard, R. L., Marhan, S. & Niklaus, P. A. Effects of drought and N-fertilization on N cycling in two grassland soils. Oecologia 171, 705–717 (2013).ADS 
PubMed 
Article 

Google Scholar 
Inatomi, M., Hajima, T. & Ito, A. Fraction of nitrous oxide production in nitrification and its effect on total soil emission: A meta-analysis and global-scale sensitivity analysis using a process-based model. Plos One 14, e0219159 (2019).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Li, Z. et al. Global patterns and controlling factors of soil nitrification rate. Glob. Change Biol. 26, 4147–4157 (2020).ADS 
Article 

Google Scholar 
Reichenau, T. G., Klar, C. W. & Schneider, K. Effects of Climate Change on Nitrate Leaching. In Regional Assessment of Global Change Impacts: The Project GLOWA-Danube (eds Mauser, W. & Prasch, M.) 623–629 (Springer, 2016).He, W. et al. Climate change impacts on crop yield, soil water balance and nitrate leaching in the semiarid and humid regions of Canada. PLoS ONE 13, 1–19 (2018).
Google Scholar 
Mas-Pla, J. & Menció, A. Groundwater nitrate pollution and climate change: learnings from a water balance-based analysis of several aquifers in a western Mediterranean region (Catalonia). Environ. Sci. Pollut. Res. 26, 2184–2202 (2019).CAS 
Article 

Google Scholar 
Stuart, M. E., Gooddy, D. C., Bloomfield, J. P. & Williams, A. T. A review of the impact of climate change on future nitrate concentrations in groundwater of the UK. Sci. Total Environ. 409, 2859–2873 (2011).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Frank, D. et al. Effects of climate extremes on the terrestrial carbon cycle: Concepts, processes and potential future impacts. Glob. Change Biol. 21, 2861–2880 (2015).ADS 
Article 

Google Scholar 
Mitchell, R. A., Mitchell, V. J., Driscoll, S. P., Franklin, J. & Lawlor, D. W. Effects of increased CO2 concentration and temperature on growth and yield of winter wheat at two levels of nitrogen application. Plant, Cell Environ. 16, 521–529 (1993).CAS 
Article 

Google Scholar 
Eisenhauer, N., Cesarz, S., Koller, R., Worm, K. & Reich, P. B. Global change belowground: Impacts of elevated CO 2, nitrogen, and summer drought on soil food webs and biodiversity. Glob. Change Biol. 18, 435–447 (2012).ADS 
Article 

Google Scholar 
Ri, X. & Prentice, I. C. Terrestrial nitrogen cycle simulation with a dynamic global vegetation model. Glob. Change Biol. 14, 1745–1764 (2008).ADS 
Article 

Google Scholar 
Ri, X., Prentice, I. C., Spahni, R. & Niu, H. S. Modelling terrestrial nitrous oxide emissions and implications for climate feedback. N. Phytologist 196, 472–488 (2012).Article 
CAS 

Google Scholar 
Giltrap, D. L. & Ausseil, A.-G. E. Upscaling NZ-DNDC using a regression based meta-model to estimate direct N2O emissions from New Zealand grazed pastures. Sci. Total Environ. 539, 221–230 (2016).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Thompson, R. L. et al. TransCom N2O model inter-comparison – Part 1: Assessing the influence of transport and surface fluxes on tropospheric N2O variability. Atmos. Chem. Phys. 14, 4349–4368 (2014).ADS 
Article 

Google Scholar 
Thompson, R. L. et al. TransCom N2O model inter-comparison – Part 2: Atmospheric inversion estimates of N2O emissions. Atmos. Chem. Phys. 14, 6177–6194 (2014).ADS 
Article 
CAS 

Google Scholar 
Thompson, R. L. et al. Acceleration of global N2O emissions seen from two decades of atmospheric inversion. Nat. Clim. Change, 8, (2019).Houlton, B. Z. & Bai, E. Imprint of denitrifying bacteria on the global terrestrial biosphere. Proc. Natl Acad. Sci. USA 106, 21713–21716 (2009).ADS 
CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Bai, E., Houlton, B. Z. & Wang, Y. P. Isotopic identification of nitrogen hotspots across natural terrestrial ecosystems. Biogeosciences 9, 3287–3304 (2012).ADS 
CAS 
Article 

Google Scholar 
Craine, J. M. et al. Ecological interpretations of nitrogen isotope ratios of terrestrial plants and soils. Plant Soil 396, 1–26 (2015).CAS 
Article 

Google Scholar 
Craine, J. M. et al. Convergence of soil nitrogen isotopes across global climate gradients. Sci. Rep. 5, 1–8 (2015).
Google Scholar 
Toyoda, S. et al. Decadal time series of tropospheric abundance of N2O isotopomers and isotopologues in the northern hemisphere obtained by the long-term observation at Hateruma Island, Japan. J. Geophys. Res. – Atmospheres 118, 1–13 (2013).
Google Scholar 
Harris, E. et al. Tracking nitrous oxide emission processes at a suburban site with semicontinuous, in situ measurements of isotopic composition. J. Geophys. Res. – Atmospheres 122, 1–21 (2017).CAS 

Google Scholar 
Harris, E. et al. Denitrifying pathways dominate nitrous oxide emissions from managed grassland during drought and rewetting. Sci. Adv. 7, eabb7118 (2021).ADS 
CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Yu, L. et al. Atmospheric nitrous oxide isotopes observed at the high-altitude research station Jungfraujoch, Switzerland. Atmos. Chem. Phys. 20, 6495–6519 (2020).ADS 
CAS 
Article 

Google Scholar 
Sowers, T., Rodebaugh, A., Yoshida, N. & Toyoda, S. Extending records of the isotopic composition of atmospheric N2O back to 1800 A.D. from air trapped in snow at the South Pole and the Greenland Ice Sheet Project II ice core. Glob. Biogeochem. Cycles 16, 1129 (2002).ADS 
Article 
CAS 

Google Scholar 
Scheer, C., Fuchs, K., Pelster, D. E. & Butterbach-Bahl, K. Estimating global terrestrial denitrification from measured N2O:(N2O + N2) product ratios. Curr. Opin. Environ. Sustainability 47, 72–80 (2020).Article 

Google Scholar 
Pilegaard, K. Processes regulating nitric oxide emissions from soils. Philos. Trans. R. Soc. B: Biol. Sci. 368, 1–8, (2013).Thompson, R. Documentation of N2O flux service: Description of the N2O inversion production chain. Technical report, Copernicus Atmospheric Monitoring Service, CAMS73_2018SC2 -Documentation of N2O flux service (2021).Voigt, C. et al. Nitrous oxide emissions from permafrost-affected soils. Nat. Rev. Earth Environ. 1, 420–434 (2020).ADS 
CAS 
Article 

Google Scholar 
Pan, B., Lam, S. K., Wang, E., Mosier, A. & Chen, D. New approach for predicting nitrification and its fraction of N2O emissions in global terrestrial ecosystems. Environ. Res. Lett. 16, (2021).Corbeels, M., Hofman, G. & Van Cleemput, O. Fate of fertiliser N applied to winter wheat growing on a Vertisol in a Medditerranean environment. Nutrient Cycl. Agroecosystems 53, 249–258 (1999).Article 

Google Scholar 
Jenkinson, D. S., Poulton, P. R., Johnston, A. E. & Powlson, D. S. Turnover of Nitrogen-15-Labeled Fertilizer in Old Grassland. Soil Sci. Soc. Am. J. 68, 865–875 (2004).ADS 
CAS 
Article 

Google Scholar 
Gardner, J. B. & Drinkwater, L. E. The fate of nitrogen in grain cropping systems: A meta-analysis of 15N field experiments. Ecol. Appl. 19, 2167–2184 (2009).PubMed 
Article 

Google Scholar 
Smith, W. et al. Towards an improved methodology for modelling climate change impacts on cropping systems in cool climates. Sci. Total Environ. 728, 138845 (2020).ADS 
CAS 
PubMed 
Article 

Google Scholar 
IPCC. Climate Change 2014: Synthesis Report. Contribution of Working Groups I, II and III to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. (IPCC, Geneva, Switzerland, 2014).Butterbach-Bahl, K., Baggs, E. M., Dannenmann, M., Kiese, R. & Zechmeister-Boltenstern, S. Nitrous oxide emissions from soils: how well do we understand the processes and their controls? Philos. Trans. R. Soc. Lond. Ser. B, Biol. Sci. 368, 1–13 (2013).Congreves, K. A., Wagner-Riddle, C., Si, B. C. & Clough, T. J. Nitrous oxide emissions and biogeochemical responses to soil freezing-thawing and drying-wetting. Soil Biol. Biochem. 117(October 2017), 5–15 (2018).Wagner-Riddle, C. et al. Globally important nitrous oxide emissions from croplands induced by freeze-thaw cycles. Nat. Geosci. 10, 279–283 (2017).ADS 
CAS 
Article 

Google Scholar 
Byers, E., Bleken, M. A. & Dörsch, P. Winter N2O accumulation and emission in sub-boreal grassland soil depend on clover proportion and soil ph. Environ. Res. Commun. 3, (2021).Doersch, P., Sturite, I. & Trier Kjaer, S. High off-season nitrous oxide emissions negate potential soil C-gain from cover crops in boreal cereal cropping (EGU22-3066). EGU General Assembly 2022, https://doi.org/10.5194/egusphere-egu22-3066 (2022).Prokopiou, M. et al. Constraining N2O emissions since 1940 using firn air isotope measurements in both hemispheres. Atmos. Chem. Phys. 17, 4539–4564 (2017).ADS 
CAS 
Article 

Google Scholar 
Yu, L., Harris, E., Lewicka-Szczebak, D. & Mohn J. What can we learn from N2O isotope data? Analytics, processes and modelling. Rap. Commun. Mass Spectr. 34, 1–13 (2020).Smith, K., Thomson, P., Clayton, H., Mctaggart, I. & Conen, F. Effects of temperature, water content and nitrogen fertilisation on emissions of nitrous oxide by soils. Atmos. Environ. 32, 3301–3309 (1998).ADS 
CAS 
Article 

Google Scholar 
Yao, Z. et al. Soil-atmosphere exchange potential of NO and N2O in different land use types of Inner Mongolia as affected by soil temperature, soil moisture, freeze-thaw, and drying-wetting events. J. Geophys. Res. – Atmospheres 115, 1–17 (2010).
Google Scholar 
Cantarel, A. A. M. et al. Four years of experimental climate change modifies the microbial drivers of N 2O fluxes in an upland grassland ecosystem. Glob. Change Biol. 18, 2520–2531 (2012).ADS 
Article 

Google Scholar 
Zhang, Y. et al. Temperature effects on N2O production pathways in temperate forest soils. Sci. Total Environ. 691, 1127–1136 (2019).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Wang, Q. et al. Data-driven estimates of global nitrous oxide emissions from croplands. Natl Sci. Rev. 7, 441–452 (2020).CAS 
PubMed 
Article 

Google Scholar 
Rütting, T., Cizungu Ntaboba, L., Roobroeck, D., Bauters, M., Huygens, D. & Boeckx, P. Leaky nitrogen cycle in pristine African montane rainforest soil. Glob. biogeochemical cycles 29, 1754–1762 (2015).ADS 
Article 
CAS 

Google Scholar 
Brookshire, E. N., Gerber, S., Greene, W., Jones, R. T. & Thomas, S. A. Global bounds on nitrogen gas emissions from humid tropical forests. Geophys. Res. Lett. 44, 2502–2510 (2017).ADS 
CAS 
Article 

Google Scholar 
Homyak, P. M. et al. Aridity and plant uptake interact to make dryland soils hotspots for nitric oxide (NO) emissions. PNAS 113, E2608–E2616 (2016).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
IPCC. 2006 IPCC Guidelines for National Greenhouse Gas Inventories. (IGES, Japan, 2006).Davidson, E. A., Suddick, E. C., Rice, C. W. & Prokopy, L. S. More Food, Low Pollution (Mo Fo Lo Po): A Grand Challenge for the 21st Century. J. Environ. Qual. 44, 305–311 (2015).CAS 
PubMed 
Article 

Google Scholar 
Cui, X. et al. Global mapping of crop-specific emission factors highlights hotspots of nitrous oxide mitigation. Nature Food, In press, (2021).McDaniel, M. D., Mas-Pla, J. & Kaye, M. W. Do “hot moments” become hotter under climate change? Soil nitrogen dynamics from a climate manipulation experiment in a post-harvest forest. Biogeochemistry. https://doi.org/10.1007/s10533-014-0001-3 (2014).Yu, G. et al. Stabilization of atmospheric nitrogen deposition in China over the past decade. Nat. Geosci. 12, 424–429 (2019).ADS 
CAS 
Article 

Google Scholar 
New, M., Lister, D., Hulme, M. & Makin, I. A high-resolution data set of surface climate over global land areas. Clim. Res. 21, 1–25 (2002).Article 

Google Scholar 
FAO, IIASA, ISRIC, ISSCAS, and JRC. Harmonized World Soil Database (version 1.2). (Technical report, FAO, Rome, Italy and IIASA, Laxenburg, Austria, 2012).Hiederer, R. & Köchy M. Global soil organic carbon estimates and the harmonized world soil database. EUR 25225EN (2012).Trabucco, A. & Zomer, R. Global Aridity Index and Potential Evapo-Transpiration (ET0) Climate Database v2, https://doi.org/10.6084/m9.figshare.7504448.v3 (2019).Slessarev, E. W. et al. Water balance creates a threshold in soil pH at the global scale. Nature 540, 567–569 (2016).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Zinke, P., Stangenberger, A., Post, W., Emanual, E. & Olson, J. WORLDWIDE ORGANIC SOIL CARBON AND NITROGEN DATA. Technical report, Oak Ridge National Laboratory, https://cdiac.ess-dive.lbl.gov/ndps/ndp018.html (2004).Kowalczyk, E. A., Wang, Y. P. & Law, R. M. The CSIRO Atmosphere Biosphere Land Exchange (CABLE) model for use in climate models and as an offline model. CSIRO Mar. Atmos. Res. Pap. 13, 1–42 (2006).
Google Scholar 
Houlton, B. Z., Wang, Y. P., Vitousek, P. M. & Field, C. B. A unifying framework for dinitrogen fixation in the terrestrial biosphere. Nature 454, 327–330 (2008).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Chen, C. et al. Nitrogen isotopic composition of plants and soil in an arid mountainous terrain: South slope versus north slope. Biogeosciences 15, 369–377 (2018).ADS 
CAS 
Article 

Google Scholar 
Brenner, D., Amundson, R., Baisden, T., Kendall, C. & Harden, J. N variation with time in a California annual grassland ecosystem. Geochimica et. Cosmochimica Acta. 65, 4171–4186 (2001).ADS 
CAS 
Article 

Google Scholar 
Xu, Y., He, J., Cheng, W., Xing, X. & Li, L. Natural 15N abundance in soils and plants in relation to N cycling in a rangeland in Inner Mongolia. J. Plant Ecol. 3, 201–207 (2010).Article 

Google Scholar 
Inglett, P. W., Reddy, K. R., Newman, S. & Lorenzen, B. Increased soil stable nitrogen isotopic ratio following phosphorus enrichment: Historical patterns and tests of two hypotheses in a phosphorus-limited wetland. Oecologia 153, 99–109 (2007).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Bissett, A. et al. Introducing BASE: the Biomes of Australian Soil Environments soil microbial diversity database. GigaScience, 5, (2016).Bauters, M. et al. Functional Composition of Tree Communities Changed Topsoil Properties in an Old Experimental Tropical Plantation. Ecosystems 20, 861–871 (2017).CAS 
Article 

Google Scholar 
Bauters, M. et al. Parallel functional and stoichiometric trait shifts in South American and African forest communities with elevation. Biogeosciences 14, 5313–5321 (2017).ADS 
CAS 
Article 

Google Scholar 
Bauters, M. et al. Contrasting nitrogen fluxes in African tropical forests of the Congo Basin. Ecol. Monograp. 89, 1–17 (2019).Bauters, M. et al. Long-term recovery of the functional community assembly and carbon pools in an African tropical forest succession. Biotropica 51, 319–329 (2019).Article 

Google Scholar 
Gallarotti, N. et al. In-depth analysis of N2O fluxes in tropical forest soils of the Congo Basin combining isotope and functional gene analysis. ISME J. (2021).Barthel, M. et al. Low N2O and variable CH4 fluxes from tropical forest soils of the Congo Basin. Nat. Commun. 13, 1–8 (2022).Article 
CAS 

Google Scholar 
Baumgartner, S. et al. Stable isotope signatures of soil nitrogen on an environmental-geomorphic gradient within the Congo Basin. Soil 7, 83–94 (2021).ADS 
CAS 
Article 

Google Scholar 
Chollet, F. Keras, Keras package for Python https://keras.io (2015).IUPAC. Compendium of Chemical Terminology, 2nd ed. (the “Gold Book”), online version created by S.J. Chalk. Blackwell Science Ltd, https://doi.org/10.1351/goldbook (2019).Yu, L. et al. Constraining global N2O budgets with decadal trends of multiple isotope signatures. In preparation, (2022).Machida, T., Nakazawa, T., Fujii, Y., Aoki, S. & Watanabe, O. Increase in the atmospheric nitrous oxide concentration during the last 250 years. Geophys. Res. Lett. 22, 2921–2924 (1995).ADS 
CAS 
Article 

Google Scholar 
Rubino, M. et al. Revised records of atmospheric trace gases CO2, CH4, N2O, and δ13C-CO2 over the last 2000 years from Law Dome, Antarctica. Earth Syst. Sci. Data 11, 473–492 (2019).ADS 
Article 

Google Scholar 
Dorich, C. et al. Improving N2O emission estimates with the global N2O database. Curr. Opin. Environ. Sustainability 47, 13–20 (2020).Article 

Google Scholar 
Mariotti, A. et al. Experimental-determination of Nitrogen Kinetic Isotope Fractionation – Some Principles – Illustration For the Denitrification and Nitrification Processes. Plant Soil 62, 413–430 (1981).CAS 
Article 

Google Scholar 
Möbius, J. Isotope fractionation during nitrogen remineralization (ammonification): Implications for nitrogen isotope biogeochemistry. Geochimica et. Cosmochimica Acta. 105, 422–432 (2013).ADS 
Article 
CAS 

Google Scholar 
Stern, L., Baisden, W. T. & Amundson, R. Processes controlling the oxygen isotope ratio of soil CO2: Analytic and numerical modeling. Geochimica Et. Cosmochimica Acta. 63, 799–814 (1999).ADS 
CAS 
Article 

Google Scholar 
Denk, T. R. A. et al. The nitrogen cycle: A review of isotope effects and isotope modeling approaches. Soil Biol. Biochem. 105, 121–137 (2017).CAS 
Article 

Google Scholar 
Rohe, L. et al. Comparing modified substrate induced respiration with selective inhibition (SIRIN) and N2O isotope approaches to estimate fungal contribution to denitrification in three arable soils under anoxic conditions. Biogeosciences, 18, 4629–4650, https://doi.org/10.5194/bg-18-4629-2021 (2021).Wei, J. et al. N2O and NOx emissions by reactions of nitrite with soil organic matter of a Norway spruce forest. Biogeochemistry 132, 325–342 (2017).CAS 
Article 

Google Scholar 
Clough, T. J. et al. Influence of soil moisture on codenitrification fluxes from a urea-affected pasture soil. Sci. Rep. 7, 1–12 (2017).CAS 
Article 

Google Scholar 
Bai, E. & Houlton, B. Z. Coupled isotopic and process-based modeling of gaseous nitrogen losses from tropical rain forests. Glob. Biogeochemical Cycles 23, 1–10 (2009).
Google Scholar 
Wen, Y. et al. Disentangling gross N2O production and consumption in soil. Sci. Rep. 6, 1–8 (2016).Article 
CAS 

Google Scholar 
Zhang, Y., Liu, X. J., Fangmeier, A., Goulding, K. T. & Zhang, F. S. Nitrogen inputs and isotopes in precipitation in the North China Plain. Atmos. Environ. 42, 1436–1448 (2008).ADS 
CAS 
Article 

Google Scholar 
Unkovich, M. Isotope discrimination provides new insight into biological nitrogen fixation. N. Phytologist 198, 643–646 (2013).CAS 
Article 

Google Scholar 
Beyn, F., Matthias, V., Aulinger, A. & Dähnke, K. Do N-isotopes in atmospheric nitrate deposition reflect air pollution levels? Atmos. Environ. 107, 281–288 (2015).ADS 
CAS 
Article 

Google Scholar 
Vereecken, H. et al. Modeling Soil Processes: Review, Key challenges and New Perspectives. Vadose Zone J. 15, 1–57 (2016).CAS 

Google Scholar 
Lamarque, J. F. et al. Multi-model mean nitrogen and sulfur deposition from the atmospheric chemistry and climate model intercomparison project (ACCMIP): Evaluation of historical and projected future changes. Atmos. Chem. Phys. 13, 7997–8018 (2013).ADS 
Article 
CAS 

Google Scholar 
Schlesinger, W. H. On the fate of anthropogenic nitrogen. Proc. Natl Acad. Sci. USA 106, 203–208 (2009).ADS 
CAS 
PubMed 
Article 

Google Scholar 
Kim, D. G., Hernandez-Ramirez, G. & Giltrap, D. Linear and nonlinear dependency of direct nitrous oxide emissions on fertilizer nitrogen input: A meta-analysis. Agriculture, Ecosyst. Environ. 168, 53–65 (2013).CAS 
Article 

Google Scholar 
Scheer, C. et al. Addressing nitrous oxide: An often ignored climate and ozone threat. Tech. Rep. (2019).Hu, H. W., Chen, D. & He, J. Z. Microbial regulation of terrestrial nitrous oxide formation: Understanding the biological pathways for prediction of emission rates. FEMS Microbiol. Rev. 39, 729–749 (2015).CAS 
PubMed 
Article 

Google Scholar 
Zaehle, S. Terrestrial nitrogen-carbon cycle interactions at the global scale. Philos. Trans. R. Soc. B: Biol. Sci. 368, 1–9 (2013).Jones, P. et al. Hemispheric and large-scale land surface air temperature variations: An extensive revision and an update to 2010. J. Geophys. Res. 117, D05127 (2012).ADS 

Google Scholar 
Olivier, J. & Berdowski, J. EDGAR 3.x by RIVM/TNO. In The Climate System (eds Berdowski, R. G. J. & Heij, B.) 33–77. (Swets and Zeitlinger Publishers, 2001).Crippa, M. et al. High resolution temporal profiles in the Emissions Database for Global Atmospheric Research. Sci. Data 7, 1–17 (2020).Article 

Google Scholar 
Bateman, A. S. & Kelly, S. D. Fertilizer nitrogen isotope signatures. Isotopes Environ. Health Stud. 43, 237–247 (2007).CAS 
PubMed 
Article 

Google Scholar 
Savard, M. M. et al. Nitrate isotopes unveil distinct seasonal N-sources and the critical role of crop residues in groundwater contamination. J. Hydrol. 381, 134–141 (2010).ADS 
CAS 
Article 

Google Scholar 
Bowman, K. P. & Cohen, P. J. Interhemispheric exchange by seasonal modulation of the Hadley circulation. J. Atmos. Sci. 54, 2045–2059 (1997).ADS 
Article 

Google Scholar 
Moseman-Valtierra, S. et al. Short-term nitrogen additions can shift a coastal wetland from a sink to a source of N2O. Atmos. Environ. 45, 4390–4397 (2011).ADS 
CAS 
Article 

Google Scholar 
Brase, L., Bange, H. W., Lendt, R., Sanders, T. & Dähnke, K. High Resolution Measurements of Nitrous Oxide (N2O) in the Elbe Estuary. Front. Mar. Sci. 4, 1–11 (2017).Article 

Google Scholar 
Wells, N. S. et al. Estuaries as Sources and Sinks of N2O Across a Land Use Gradient in Subtropical Australia. Glob. Biogeochemical Cycles 32, 877–894 (2018).ADS 
CAS 
Article 

Google Scholar 
Rayner, P. Data Assimilation using an ensemble of models: A hierarchical approach. Atmos. Chem. Phys. 20, 1–13 (2020).Article 
CAS 

Google Scholar 
Met Office. Cartopy: a cartographic python library with a Matplotlib interface (https://scitools.org.uk/cartopy), (2015). More

Effects of temperature on total daily locomotor activitiesTo understand the effect of temperature on the daily behavior of Drosophila species distributed in different temperature regions, we examined the daily locomotor activity at different temperatures in the following 11 sequenced Drosophila species: cosmopolitan (D. melanogaster and D. simulans), tropical (D. ananassae, D. erecta, D. yakuba, and D. sechellia), subtropical (D. willistoni and D. mojavensis), and temperate (D. persimilis, D. pseudoobscura, and D. virilis) species. Using the Drosophila Activity Monitor system25, we were able to analyze the amount of daily locomotor activity quantitatively at five experimental temperatures, i.e., 17 °C, 20 °C, 23 °C, 26 °C, and 29 °C. As the viability of the adults of D. persimilis and D. pseudoobscura was low at 29 °C, these two species were analyzed at only four experimental temperatures. First, we compared the amount of daily locomotor activities among these Drosophila species (Supplementary Fig. 1). The ranges of the total daily activity were quite diverse in these species (Kruskal–Wallis test: χ2 = 833.18, p  More

Basic information of intervieweesResults of the descriptive analysis summarized in Table 2 show that more than half of the respondents were males (69%) and were on average 41.3 years old while more than 32 years of farming experience. The study area is comprised of multiple ethnic groups (Han, Tibetan, Yugur, Mongolian, Hui, etc.). In most cases, the main livelihood activity of the Ethnic Minorities (Tibetan, Yugur, Mongolian, Hui, etc.) is livestock, while Han people main livelihood activity is farming. The majority of respondents (64%) were minority nationality. The vast majority of the agro-pastoralists (86%) have a primary school education or above, even though only 1% of them have Undergraduate education or Above. The results also reveal that 92% of respondents have access to weather information. The average cultivated land Per household is 10.23 Mu and Grassland is 156.21 Mu, respectively. The average per household income is RMB78000, and agricultural income is RMB52000.Table 2 Descriptive statistics of agro-pastoralist characteristics.Full size tableDue to their long-term farming experience, the agro-pastoralists were expected to have a high-level of understanding of local climate knowledge. Also contributing to this could be the information they receive about climate change and for some, the associated training through agro-pastoralists’ associations. Therefore, they also have a propensity to adapt to adverse conditions resulting from climate change impacts. In addition, the high-level of farming experience, the cultivated-land size, grassland size, Credit loan, Insurance, Village cadres all have a positive impact on the level of agro-pastoralists’ adaptation to new climate scenarios.However, the education level and cadres experience may be the major limiting factors for adopting specific long-term adaptation strategies. Ethnicity and gender are also expected to be key factors influencing awareness and adaptation to climate change. There are differences in relative perception intensity between Ethnic Minority and Han because of their cultural ecology (the main livelihood activity of minorities nationality is livestock, while Han main livelihood activity is farming.). In terms of gender, women in rural areas are less mobile and have less access to information and rights. They are also heavily involved in domestic work. However, men may have easier access to information (socializing, going out to work, etc.) Therefore, male headed households are expected to be more likely to adapt to the impact of climate change.Climate change trend in the study areaFigure 2 shows the trend of annual precipitation, annual rainfall and annual snow at different meteorological stations in the study area. As shown in the Fig. 2, precipitation, rainfall and snow show an increasing trend, but the increase range of snow (0.0325–0.375/a) is significantly lower than that of precipitation (1.22–3.1/a) and rainfall (1.04–2.81/a). Similarly, through the inspection, it is found that the multi-collinearity among precipitation, rainfall and snow at each meteorological station is obvious (most R2  > 0.5, and p  More

White, R., Murray, S. & Rohweder, M. Pilot Analysis of Global Ecosystems: Grassland Ecosystems Technical Report (World Resources Institute, 2000).Thornton, P. K. Livestock production: recent trends, future prospects. Philos. Trans. R. Soc. B 365, 2853–2867 (2010).Article 

Google Scholar 
Estes, J. A. et al. Trophic downgrading of planet Earth. Science 333, 301–306 (2011).CAS 
PubMed 
Article 

Google Scholar 
Peñuelas, J. et al. Human-induced nitrogen–phosphorus imbalances alter natural and managed ecosystems across the globe. Nat. Commun. 4, 2934 (2013).PubMed 
Article 
CAS 

Google Scholar 
Asner, G. P. et al. Physical and biogeochemical controls over terrestrial ecosystem responses to nitrogen deposition. Biogeochemistry 54, 1–39 (2001).CAS 
Article 

Google Scholar 
Galloway, J. N. et al. Nitrogen cycles: past, present, and future. Biogeochemistry 70, 153–226 (2004).CAS 
Article 

Google Scholar 
Ripple, W. J. et al. Collapse of the world’s largest herbivores. Sci. Adv. 1, e1400103 (2015).PubMed 
PubMed Central 
Article 

Google Scholar 
Borer, E. T., Grace, J. B., Harpole, W. S., MacDougall, A. S. & Seabloom, E. W. A decade of insights into grassland ecosystem responses to global environmental change. Nat. Ecol. Evol. 1, 0118 (2017).Article 

Google Scholar 
Díaz, S. et al. Plant trait responses to grazing—a global synthesis. Glob. Change Biol. 13, 313–341 (2007).Article 

Google Scholar 
Cingolani, A. M., Noy-Meir, I. & Díaz, S. Grazing effects on rangeland diversity: a synthesis of contemporary models. Ecol. Appl. 15, 757–773 (2005).Article 

Google Scholar 
Milchunas, D. G., Sala, O. E. & Lauenroth, W. K. A generalized model of the effects of grazing by large herbivores on grassland community structure. Am. Nat. 132, 87–106 (1988).Article 

Google Scholar 
Osem, Y., Perevolotsky, A. & Kigel, J. Site productivity and plant size explain the response of annual species to grazing exclusion in a Mediterranean semi-arid rangeland. J. Ecol. 92, 297–309 (2004).Article 

Google Scholar 
Gao, J. & Carmel, Y. Can the intermediate disturbance hypothesis explain grazing–diversity relations at a global scale? Oikos 129, 493–502 (2020).Article 

Google Scholar 
Bakker, E. S., Ritchie, M. E., Olff, H., Milchunas, D. G. & Knops, J. M. Herbivore impact on grassland plant diversity depends on habitat productivity and herbivore size. Ecol. Lett. 9, 780–788 (2006).PubMed 
Article 

Google Scholar 
Mack, R. N. & Thompson, J. N. Evolution in steppe with few large, hooved mammals. Am. Nat. 119, 757–773 (1982).Article 

Google Scholar 
Axelrod, D. I. Rise of the grassland biome, central North America. Bot. Rev. 51, 163–201 (1985).Article 

Google Scholar 
Noy-Meir, I., Gutman, M. & Kaplan, Y. Responses of Mediterranean grassland plants to grazing and protection. J. Ecol. 77, 290–310 (1989).Article 

Google Scholar 
Olff, H. & Ritchie, M. E. Effects of herbivores on grassland plant diversity. Trends Ecol. Evol. 13, 261–265 (1998).CAS 
PubMed 
Article 

Google Scholar 
Proulx, M. & Mazumder, A. Reversal of grazing impact on plant species richness in nutrient-poor vs. nutrient-rich ecosystems. Ecology 79, 2581–2592 (1998).Article 

Google Scholar 
Westoby, M., Walker, B. & Noy-Meir, I. Opportunistic management for rangelands not at equilibrium. J. Range Manag. 42, 266–274 (1989).Article 

Google Scholar 
Prober, S. M., Standish, R. J. & Wiehl, G. After the fence: vegetation and topsoil condition in grazed, fenced and benchmark eucalypt woodlands of fragmented agricultural landscapes. Aust. J. Bot. 59, 369–381 (2011).Article 

Google Scholar 
Seabloom, E. W., Harpole, W. S., Reichman, O. J. & Tilman, D. Invasion, competitive dominance, and resource use by exotic and native California grassland species. Proc. Natl Acad. Sci. USA 100, 13384–13389 (2003).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Price, J. N., Schultz, N. L., Hodges, J. A., Cleland, M. A. & Morgan, J. W. Land-use legacies limit the effectiveness of switches in disturbance type to restore endangered grasslands. Restor. Ecol. 29, e13271 (2021).Article 

Google Scholar 
Hobbs, R. J. & Huenneke, L. F. Disturbance, diversity, and invasion: implications for conservation. Conserv. Biol. 6, 324–337 (1992).Article 

Google Scholar 
MacDougall, A. S. et al. The Neolithic plant invasion hypothesis: the role of preadaptation and disturbance in grassland invasion. New Phytol. 220, 94–103 (2018).PubMed 
Article 

Google Scholar 
Mörsdorf, M. A., Ravolainen, V. T., Yoccoz, N. G., Thórhallsdóttir, T. E. & Jónsdóttir, I. S. Decades of recovery from sheep grazing reveal no effects on plant diversity patterns within Icelandic tundra landscapes. Front. Ecol. Evol. 8, 602538 (2021).Mack, R. N. in Biological Invasions: A Global Perspective (eds Drake, J. A. et al.) 155–180 (John Wiley, 1989).Sinkins, P. A. & Otfinowski, R. Invasion or retreat? The fate of exotic invaders on the northern prairies, 40 years after cattle grazing. Plant Ecol. 213, 1251–1262 (2012).Article 

Google Scholar 
Stahlheber, K. A., D’Antonio, C. M. & Tyler, C. M. Livestock exclusion impacts on oak savanna habitats—differential responses of understory and open habitats. Rangel. Ecol. Manag. 70, 316–323 (2017).Article 

Google Scholar 
Koerner, S. E. et al. Change in dominance determines herbivore effects on plant biodiversity. Nat. Ecol. Evol. 2, 1925–1932 (2018).PubMed 
Article 

Google Scholar 
Gao, J. & Carmel, Y. A global meta-analysis of grazing effects on plant richness. Agric. Ecosyst. Environ. 302, 107072 (2020).Article 

Google Scholar 
Borer, E. T. et al. Finding generality in ecology: a model for globally distributed experiments. Methods Ecol. Evol. 5, 65–73 (2014).Article 

Google Scholar 
Borer, E. T. et al. Herbivores and nutrients control grassland plant diversity via light limitation. Nature 508, 517–520 (2014).CAS 
PubMed 
Article 

Google Scholar 
Milchunas, D. G. & Lauenroth, W. K. Quantitative effects of grazing on vegetation and soils over a global range of environments. Ecol. Monogr. 63, 327–366 (1993).Article 

Google Scholar 
Mortensen, B. et al. Herbivores safeguard plant diversity by reducing variability in dominance. J. Ecol. 106, 101–112 (2018).CAS 
Article 

Google Scholar 
Chen, Q. et al. Small herbivores slow down species loss up to 22 years but only at early successional stage. J. Ecol. 107, 2688–2696 (2019).Article 

Google Scholar 
Lunt, I. D., Eldridge, D. J., Morgan, J. W. & Witt, G. B. A framework to predict the effects of livestock grazing and grazing exclusion on conservation values in natural ecosystems in Australia. Aust. J. Bot. 55, 401–415 (2007).Article 

Google Scholar 
Anderson, T. M. et al. Herbivory and eutrophication mediate grassland plant nutrient responses across a global climatic gradient. Ecology 99, 822–831 (2018).PubMed 
Article 

Google Scholar 
Seabloom, E. W. et al. Plant species’ origin predicts dominance and response to nutrient enrichment and herbivores in global grasslands. Nat. Commun. 6, 7710 (2015).CAS 
PubMed 
Article 

Google Scholar 
Barrio, I. C. et al. The sheep in wolf’s clothing? Recognizing threats for land degradation in Iceland using state-and-transition models. Land Degrad. Dev. 29, 1714–1725 (2018).Article 

Google Scholar 
Eldridge, D. J., Poore, A. G. B., Ruiz-Colmenero, M., Letnic, M. & Soliveres, S. Ecosystem structure, function, and composition in rangelands are negatively affected by livestock grazing. Ecol. Appl. 26, 1273–1283 (2016).PubMed 
Article 

Google Scholar 
Seabloom, E. W. et al. Increasing effects of chronic nutrient enrichment on plant diversity loss and ecosystem productivity over time. Ecology 102, e03218 (2021).PubMed 
Article 

Google Scholar 
Fay, P. A. et al. Grassland productivity limited by multiple nutrients. Nat. Plants 1, 15080 (2015).CAS 
PubMed 
Article 

Google Scholar 
Yuan, Z. Y., Jiao, F., Li, Y. H. & Kallenbach, R. L. Anthropogenic disturbances are key to maintaining the biodiversity of grasslands. Sci. Rep. 6, 22132 (2016).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Borer, E. T. et al. Nutrients cause grassland biomass to outpace herbivory. Nat. Commun. 11, 6036 (2020).CAS 
PubMed 
PubMed Central 
Article 

Google Scholar 
Seabloom, E. W. et al. Species loss due to nutrient addition increases with spatial scale in global grasslands. Ecol. Lett. 24, 2100–2112 (2021).PubMed 
Article 

Google Scholar 
Hijmans, R. J., Cameron, S. E., Parra, J. L., Jones, P. G. & Jarvis, A. Very high resolution interpolated climate surfaces for global land areas. Int. J. Climatol. 25, 1965–1978 (2005).Article 

Google Scholar 
R Core Team. R: A Language and Environment for Statistical Computing (R Foundation for Statistical Computing, 2020). More

Thomas, N. & Nigam, S. Twentieth-century climate change over Africa: seasonal hydroclimate trends and Sahara desert expansion. J. Clim. 31, 3349–3370 (2018).Article 

Google Scholar 
Maley J. in The Sahara and the Nile (eds Martin A. J. Williams and Hugues Faure) 63–86 (Balkema, 1980).deMenocal, P. B. Plio-Pleistocene African climate. Science 270, 53–59 (1995).Article 

Google Scholar 
Trauth, M. H., Larrasoaña, J. C. & Mudelsee, M. Trends, rhythms and events in Plio-Pleistocene African climate. Quat. Sci. Rev. 28, 399–411 (2009).Article 

Google Scholar 
Muhs, D. R. et al. The antiquity of the Sahara desert: new evidence from the mineralogy and geochemistry of Pliocene paleosols on the Canary Islands, Spain. Palaeogeogr. Palaeoclimatol. Palaeoecol. 533, 109245 (2019).Article 

Google Scholar 
Schuster, M. et al. The age of the Sahara desert. Science 311, 821 (2006).Article 

Google Scholar 
Zhang, Z. et al. Aridification of the Sahara desert caused by Tethys Sea shrinkage during the late Miocene. Nature 513, 401–404 (2014).Article 

Google Scholar 
Kroepelin, S. & Swezey, C. S. Revisiting the age of the Sahara desert. Science 312, 1138–1139 (2006).Article 

Google Scholar 
McQuarrie, N. & van Hinsbergen, D. J. J. Retrodeforming the Arabia–Eurasia collision zone: age of collision versus magnitude of continental subduction. Geology 41, 315–318 (2013).Article 

Google Scholar 
Allen, M. B. & Armstrong, H. A. Arabia–Eurasia collision and the forcing of mid-Cenozoic global cooling. Palaeogeogr. Palaeoclimatol. Palaeoecol. 265, 52–58 (2008).Article 

Google Scholar 
Tiedemann, R., Sarnthein, M. & Shackleton, N. J. Astronomic timescale for the Pliocene Atlantic δ18O and dust flux records of Ocean Drilling Program Site 659. Paleoceanography 9, 619–638 (1994).Article 

Google Scholar 
Tjallingii, R. et al. Coherent high- and low-latitude control of the northwest African hydrological balance. Nat. Geosci. 1, 670–675 (2008).Article 

Google Scholar 
Skonieczny, C. et al. African humid periods triggered the reactivation of a large river system in western Sahara. Nat. Commun. 6, 8751 (2015).Article 

Google Scholar 
Ruddiman. W. F. et al. (eds) Proceedings of the Ocean Drilling Program: Scientific Results Vol. 108 (ODP, 1989).Skonieczny, C. et al. Monsoon-driven Saharan dust variability over the past 240,000 years. Sci. Adv. 5, eaav1887 (2019).Article 

Google Scholar 
McGee, D., deMenocal, P. B., Winckler, G., Stuut, J. B. W. & Bradtmiller, L. I. The magnitude, timing and abruptness of changes in North African dust deposition over the last 20,000 yr. Earth Planet. Sci. Lett. 371–372, 163–176 (2013).Article 

Google Scholar 
Mulitza, S. et al. Increase in African dust flux at the onset of commercial agriculture in the Sahel region. Nature 466, 226–228 (2010).Article 

Google Scholar 
Drake, N. A., Blench, R. M., Armitage, S. J., Bristow, C. S. & White, K. H. Ancient watercourses and biogeography of the Sahara explain the peopling of the desert. Proc. Natl Acad. Sci. USA 108, 458–462 (2011).Article 

Google Scholar 
Larrasoaña, J. C., Roberts, A. P. & Rohling, E. J. Dynamics of green Sahara periods and their role in hominin evolution. PLoS ONE 8, e76514 (2013).Article 

Google Scholar 
Tierney, J. E., Pausata, F. S. R. & deMenocal, P. B. Rainfall regimes of the green Sahara. Sci. Adv. 3, e1601503 (2017).Article 

Google Scholar 
Mori, F. The earliest Saharan rock-engravings. Antiquity 48, 87–92 (1974).Article 

Google Scholar 
McGee, D., Broecker, W. S. & Winckler, G. Gustiness: the driver of glacial dustiness? Quat. Sci. Rev. 29, 2340–2350 (2010).Article 

Google Scholar 
Herbert, T. D. et al. Late Miocene global cooling and the rise of modern ecosystems. Nat. Geosci. 9, 843–847 (2016).Article 

Google Scholar 
Abell, J. T., Winckler, G., Anderson, R. F. & Herbert, T. D. Poleward and weakened westerlies during Pliocene warmth. Nature 589, 70–75 (2021).Article 

Google Scholar 
Burls, N. J. & Fedorov, A. V. Wetter subtropics in a warmer world: contrasting past and future hydrological cycles. Proc. Natl Acad. Sci. USA 114, 12888–12893 (2017).Article 

Google Scholar 
Moussa, A. et al. Lake Chad sedimentation and environments during the late Miocene and Pliocene: new evidence from mineralogy and chemistry of the Bol core sediments. J. Afr. Earth. Sci. 118, 192–204 (2016).Article 

Google Scholar 
Washington, R., Todd, M., Middleton, N. J. & Goudie, A. S. Dust‐storm source areas determined by the total ozone monitoring spectrometer and surface observations. Ann. Assoc. Am. Geographers 93, 297–313 (2003).Article 

Google Scholar 
Schepanski, K., Tegen, I. & Macke, A. Comparison of satellite based observations of Saharan dust source areas. Remote Sens. Environ. 123, 90–97 (2012).Article 

Google Scholar 
Westerhold, T. et al. An astronomically dated record of Earth’s climate and its predictability over the last 66 million years. Science 369, 1383–1387 (2020).Article 

Google Scholar 
Sarnthein, M. et al. in Geology of the Northwest African Continental Margin (eds von Rad, U. et al.) 545–604 (Springer, 1982).Jewell, A. M. et al. Three North African dust source areas and their geochemical fingerprint. Earth Planet. Sci. Lett. 554, 116645 (2021).Article 

Google Scholar 
Cerling, T. E. et al. Global vegetation change through the Miocene/Pliocene boundary. Nature 389, 153–158 (1997).Article 

Google Scholar 
Feakins, S. J. et al. Northeast African vegetation change over 12 m.y. Geology 41, 295–298 (2013).Article 

Google Scholar 
Pagani, M., Freeman, K. H. & Arthur, M. A. Late Miocene atmospheric CO2 concentrations and the expansion of C4 grasses. Science 285, 876–879 (1999).Article 

Google Scholar 
Beerling, D. J. & Osborne, C. P. The origin of the savanna biome. Glob. Change Biol. 12, 2023–2031 (2006).Article 

Google Scholar 
Polissar, P. J., Rose, C., Uno, K. T., Phelps, S. R. & deMenocal, P. Synchronous rise of African C4 ecosystems 10 million years ago in the absence of aridification. Nat. Geosci. 12, 657–660 (2019).Article 

Google Scholar 
Hoetzel, S., Dupont, L., Schefuß, E., Rommerskirchen, F. & Wefer, G. The role of fire in Miocene to Pliocene C4 grassland and ecosystem evolution. Nat. Geosci. 6, 1027–1030 (2013).Article 

Google Scholar 
Naafs, B. D. A. et al. Strengthening of North American dust sources during the late Pliocene (2.7 Ma). Earth Planet. Sci. Lett. 317–318, 8–19 (2012).Article 

Google Scholar 
Kuechler, R. R., Dupont, L. M. & Schefuß, E. Hybrid insolation forcing of Pliocene monsoon dynamics in West Africa. Clim. Past 14, 73–84 (2018).Article 

Google Scholar 
Kuechler, R. R., Schefuß, E., Beckmann, B., Dupont, L. & Wefer, G. NW African hydrology and vegetation during the last glacial cycle reflected in plant-wax-specific hydrogen and carbon isotopes. Quat. Sci. Rev. 82, 56–67 (2013).Article 

Google Scholar 
Cerling, T. E. et al. Woody cover and hominin environments in the past 6 million years. Nature 476, 51–56 (2011).Article 

Google Scholar 
Faith, J. T., Rowan, J., Du, A. & Koch, P. L. Plio-Pleistocene decline of African megaherbivores: no evidence for ancient hominin impacts. Science 362, 938–941 (2018).Article 

Google Scholar 
Potts, R. Hominin evolution in settings of strong environmental variability. Quat. Sci. Rev. 73, 1–13 (2013).Article 

Google Scholar 
Maslin, M. A. et al. East African climate pulses and early human evolution. Quat. Sci. Rev. 101, 1–17 (2014).Article 

Google Scholar 
Zollikofer, C. P. E. et al. Virtual cranial reconstruction of Sahelanthropus tchadensis. Nature 434, 755 (2005).Article 

Google Scholar 
DiMaggio, E. N. et al. Late Pliocene fossiliferous sedimentary record and the environmental context of early Homo from Afar, Ethiopia. Science 347, 1355–1359 (2015).Article 

Google Scholar 
Bobe, R. & Wood, B. Estimating origination times from the early hominin fossil record. Evol. Anthropol. 31, 92–102 (2022).Uno, K. T., Polissar, P. J., Jackson, K. E. & deMenocal, P. B. Neogene biomarker record of vegetation change in eastern Africa. Proc. Natl Acad. Sci. USA 113, 201521267 (2016).Article 

Google Scholar 
Laskar, J. et al. A long-term numerical solution for the insolation quantities of the Earth. Astron. Astrophys. 428, 261–285 (2004).Article 

Google Scholar 
Kumar, A. et al. Seasonal radiogenic isotopic variability of the African dust outflow to the tropical Atlantic Ocean and across to the Caribbean. Earth Planet. Sci. Lett. 487, 94–105 (2018).Article 

Google Scholar 
Gama, C. et al. Seasonal patterns of Saharan dust over Cape Verde—a combined approach using observations and modelling. Tellus B 67, 24410 (2015).Article 

Google Scholar 
Patey, M. D., Achterberg, E. P., Rijkenberg, M. J. & Pearce, R. Aerosol time-series measurements over the tropical Northeast Atlantic Ocean: dust sources, elemental composition and mineralogy. Mar. Chem. 174, 103–119 (2015).Article 

Google Scholar 
Skonieczny, C. et al. A three-year time series of mineral dust deposits on the West African margin: sedimentological and geochemical signatures and implications for interpretation of marine paleo-dust records. Earth Planet. Sci. Lett. 364, 145–156 (2013).Article 

Google Scholar 
Ratmeyer, V., Fischer, G. & Wefer, G. Lithogenic particle fluxes and grain size distributions in the deep ocean off northwest Africa: mplications for seasonal changes of aeolian dust input and downward transport. Deep Sea Res. 1 46, 1289–1337 (1999).Article 

Google Scholar 
Bory, A. et al. Atmospheric and oceanic dust fluxes in the northeastern tropical Atlantic Ocean: how close a coupling? Ann. Geophys. 20, 2067–2076 (2002).Article 

Google Scholar 
Chiapello, I. et al. Origins of African dust transported over the northeastern tropical Atlantic. J. Geophys. Res. Atmos. 102, 13701–13709 (1997).Article 

Google Scholar 
Stuut, J.-B. et al. Provenance of present-day eolian dust collected off NW Africa. J. Geophys. Res. Atmos. 110, D04202 (2005).Article 

Google Scholar 
Schepanski, K., Tegen, I. & Macke, A. Saharan dust transport and deposition towards the tropical northern Atlantic. Atmos. Chem. Phys. 9, 1173–1189 (2009).Article 

Google Scholar 
Caquineau, S., Gaudichet, A., Gomes, L. & Legrand, M. Mineralogy of Saharan dust transported over northwestern tropical Atlantic Ocean in relation to source regions. J. Geophys. Res. Atmos. 107, 4251 (2002).Article 

Google Scholar 
Formenti, P. et al. Regional variability of the composition of mineral dust from western Africa: results from the AMMA SOP0/DABEX and DODO field campaigns. J. Geophys. Res. Atmos. 113, D00C13 (2008).Article 

Google Scholar 
Friese, C. A., van Hateren, J. A., Vogt, C., Fischer, G. & Stuut, J.-B. W. Seasonal provenance changes in present-day Saharan dust collected in and off Mauritania. Atmos. Chem. Phys. 17, 10163 (2017).Article 

Google Scholar 
McConnell, C. L. et al. Seasonal variations of the physical and optical characteristics of Saharan dust: results from the Dust Outflow and Deposition to the Ocean (DODO) experiment. J. Geophys. Res. Atmos. 113, D14S05 (2008).Article 

Google Scholar 
Salvador, P. et al. Composition and origin of PM10 in Cape Verde: characterization of long-range transport episodes. Atmos. Environ. 127, 326–339 (2016).Article 

Google Scholar 
Skonieczny, C. et al. The 7-13 March 2006 major Saharan outbreak: multiproxy characterization of mineral dust deposited on the West African margin. J. Geophys. Res. Atmos. 116, D18210 (2011).Article 

Google Scholar 
Zhao, W., Balsam, W., Williams, E., Long, X. & Ji, J. Sr–Nd–Hf isotopic fingerprinting of transatlantic dust derived from North Africa. Earth Planet. Sci. Lett. 486, 23–31 (2018).Article 

Google Scholar 
Holz, C., Stuut, J.-B. W. & Henrich, R. Terrigenous sedimentation processes along the continental margin off NW Africa: implications from grain-size analysis of seabed sediments. Sedimentology 51, 1145–1154 (2004).Article 

Google Scholar 
Matthewson, A. P., Shimmield, G. B., Kroon, D. & Fallick, A. E. A 300 kyr high‐resolution aridity record of the North African continent. Paleoceanography 10, 677–692 (1995).Article 

Google Scholar 
Wilkens, R. H. et al. Revisiting Ceara Rise, equatorial Atlantic Ocean: isotope stratigraphy ODP leg 154 from 0 to 5 Ma. Clim. Past 13, 779–793 (2017).Article 

Google Scholar 
Manivit, H. in Proceedings of the Ocean Drilling Program: Scientific Results Vol. 108 (eds Ruddiman, W. et al.) 35–69 (ODP, 1989).Raffi, I. et al. A review of calcareous nannofossil astrobiochronology encompassing the past 25 million years. Quat. Sci. Rev. 25, 3113–3137 (2006).Article 

Google Scholar 
Ogg, J. G. in The Geologic Time Scale (eds Gradstein, F. M. et al.) 85–113 (Elsevier, 2012).Wade, B. S., Pearson, P. N., Berggren, W. A. & Pälike, H. Review and revision of Cenozoic tropical planktonic foraminiferal biostratigraphy and calibration to the geomagnetic polarity and astronomical time scale. Earth Sci. Rev. 104, 111–142 (2011).Article 

Google Scholar 
Lisiecki, L. E. & Raymo, M. E. A Pliocene–Pleistocene stack of 57 globally distributed benthic δ18O records. Paleoceanography 20, PA1003 (2005).
Google Scholar 
Grinsted, A., Moore, J. C. & Jevrejeva, S. Application of the cross wavelet transform and wavelet coherence to geophysical time series. Nonlinear Process. Geophys. 11, 561–566 (2004).Article 

Google Scholar 
Schulz, M. & Mudelsee, M. REDFIT: estimating red-noise spectra directly from unevenly spaced paleoclimatic time series. Comput. Geosci. 28, 421–426 (2002).Article 

Google Scholar 
Weltje, G. J. & Tjallingii, R. Calibration of XRF core scanners for quantitative geochemical logging of sediment cores: theory and application. Earth Planet. Sci. Lett. 274, 423–438 (2008).Article 

Google Scholar 
Weltje, G. J. et al. in Micro-XRF Studies of Sediment Cores (eds Croudace, I. W. & Rothwell, R. G.) 507–534 (Springer, 2015).Bloemsma, M. R. Development of a Modelling Framework for Core Data Integration using XRF Scanning (Delft University of Technology, 2015).Gac, J.-Y. & Kane, A. Le fleuve Sénégal: I. Bilan hydrologique et flux continentaux de matières particulaires à l’embouchure. Sci. Geol. Mem. 31, 99–130 (1986).
Google Scholar 
Scheuvens, D., Schütz, L., Kandler, K., Ebert, M. & Weinbruch, S. Bulk composition of northern African dust and its source sediments—a compilation. Earth Sci. Rev. 116, 170–194 (2013).Article 

Google Scholar 
Orange, D. & Gac, J.-Y. Bilan géochimique des apports atmosphériques en domaines sahélien et soudano-guinéen d’Afrique de l’Ouest (bassins supérieurs du Sénégal et de la Gambie). Géodynamique 5, 51–65 (1990).
Google Scholar 
Orange, D., Gac, J.-Y. & Diallo, M. I. Geochemical assessment of atmospheric deposition including Harmattan dust in continental West Africa. In Tracers in Hydrology: Proc. Yokohama Symposium (ed. Peters, N. E., Hoehn, E., Leibundgut, C., Tase, N. & Walling, D.E.) 303–312 (IAHS, 1993).Guieu, C. & Thomas, A. J. in The Impact of Desert Dust Across the Mediterranean (eds Guersoni, S. & Chester, R.) 207–216 (Springer, 1996).Criado, C. & Dorta, P. An unusual ‘blood rain’ over the Canary Islands (Spain). The storm of January 1999. J. Arid. Environ. 55, 765–783 (2003).Article 

Google Scholar 
Viana, M., Querol, X., Alastuey, A., Cuevas, E. & Rodrı́guez, S. Influence of African dust on the levels of atmospheric particulates in the Canary Islands air quality network. Atmos. Environ. 36, 5861–5875 (2002).Article 

Google Scholar 
Formenti, P., Elbert, W., Maenhaut, W., Haywood, J. & Andreae, M. O. Chemical composition of mineral dust aerosol during the Saharan Dust Experiment (SHADE) airborne campaign in the Cape Verde region, September 2000. J. Geophys. Res. Atmos. 108, 8576 (2003).Article 

Google Scholar 
Linke, C. et al. Optical properties and mineralogical composition of different Saharan mineral dust samples: a laboratory study. Atmos. Chem. Phys. 6, 3315–3323 (2006).Article 

Google Scholar 
Khiri, F., Ezaidi, A. & Kabbachi, K. Dust deposits in Souss–Massa basin, south-west of Morocco: granulometrical, mineralogical and geochemical characterisation. J. Afr. Earth. Sci. 39, 459–464 (2004).Article 

Google Scholar 
Moreno, T. et al. Geochemical variations in aeolian mineral particles from the Sahara–Sahel Dust Corridor. Chemosphere 65, 261–270 (2006).Article 

Google Scholar 
Mounkaila, M. Spectral and Mineralogical Properties of Potential Dust Sources on a Transect from the Bodélé Depresseion (Central Sahara) to the Lake Chad in the Sahel (Univ. Hohenheim, 2006).Herrmann, L., Jahn, R. & Maurer, T. Mineral dust around the Sahara—from source to sink. A review with emphasis on contributions of the German soil science community in the last twenty years. J. Plant Nutr. Soil Sci. 173, 811–821 (2010).Article 

Google Scholar 
Tiedemann, R. Acht Millionen Jahre Klimageschichte von Nordwest Afrika und Paläo-Ozeanographie des angrenzenden Atlantiks: Hochauflösende Zeitreihen von ODP-Sites 658–661 (Christian-Albrechts-Universität, 1991).Cohen, A. S., O’Nions, R. K., Siegenthaler, R. & Griffin, W. L. Chronology of the pressure–temperature history recorded by a granulite terrain. Contrib. Mineral. Petrol. 98, 303–311 (1988).Article 

Google Scholar 
Pin, C. & Zalduegui, J. S. Sequential separation of light rare-earth elements, thorium and uranium by miniaturized extraction chromatography: application to isotopic analyses of silicate rocks. Anal. Chim. Acta 339, 79–89 (1997).Article 

Google Scholar 
Vance, D. & Thirlwell, M. An assessment of mass discrimination in MC-ICPMS using Nd isotopes. Chem. Geol. 185, 227–240 (2002).Article 

Google Scholar 
Tanaka, T. et al. JNdi-1: a neodymium isotopic reference in consistency with LaJolla neodymium. Chem. Geol. 168, 279–281 (2000).Article 

Google Scholar 
Jacobsen, S. B. & Wasserburg, G. J. Sm–Nd isotopic evolution of chondrites. Earth Planet. Sci. Lett. 50, 139–155 (1980).Article 

Google Scholar 
Dietze, E. et al. An end-member algorithm for deciphering modern detrital processes from lake sediments of Lake Donggi Cona, NE Tibetan Plateau, China. Sediment. Geol. 243–244, 169–180 (2011).
Google Scholar 
Wood, S. N. Generalized Additive Models: An iIntroduction with R (CRC Press, 2017).Hammer, Ø., Harper, D. A. T. & Ryan, P. D. PAST: paleontological statistics software package for education and data analysis. Palaeontol. Electron. 4, 4 (2001).
Google Scholar 
Castillo, S. et al. Trace element variation in size-fractionated African desert dusts. J. Arid. Environ. 72, 1034–1045 (2008).Article 

Google Scholar  More